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1.
This study was carried out in Arsin (Trabzon/Turkey) in 2011. The effects of different harvest time and altitudes on the quality of the nuts have been investigated. The study was performed on Fo?a hazelnut and the harvest process has been conducted at three terms, which are on normal harvest time and ten days before and after harvest time. The harvested nuts were dried in the shade on the concrete floor until their moisture content decreased to 5?%. Some properties of nuts including yield, fruit weight, internal weight, shell thickness, and protein, oleic, and linoleic acid amounts have been investigated. As evaluated all of the fruit properties it can be concluded that 11 August is the most suitable harvest date for coast zone. On the other hand, no significant differences were obtained in the point of protein, oleic, and linoleic acid amounts for different harvest time and altitudes. 相似文献
2.
In the mid-1970s, a new apple variety named ‘Meran’ was discovered in South Tyrol (northern Italy), which harbours the largest continuous apple growing area in Europe. The cultivar was registered for varietal protection and patented in several countries, and was declared to be a cross of the varieties ‘Golden Delicious’ and ‘Morgenduft’ (synonym ‘Rome Beauty’). The parentage of ‘Meran’ has, however, been questioned, and the present study aimed to assess the descent of this cultivar by the combined use of molecular genetic and bioinformatic tools. Five accessions of ‘Meran’ were collected from three different European germplasm collections and analysed at 14 variable microsatellite DNA loci. Subsequently, computer software was used to allocate the most likely parent pair from a set of cultivars representative for the apple growing area of South Tyrol in 1975. The molecular genetic data clearly excluded ‘Morgenduft’ as a gene donor to ‘Meran’ and provided strong evidence that ‘Meran’ is a cross of the cultivars ‘Golden Delicious’ and ‘Jonathan’, confirming previous assumptions based on morphological traits of the tree and fruit. 相似文献
3.
When apples which develop low temperature breakdown (LTB) at 32° F. are moved from 32° F. to 65° F. for 3 to 5 days at about the 7th to 8th week of storage, they subsequently develop within a given period of storage less LTB than apples kept at 32° F. continuously.The respiration of apples susceptible to LTB increases steadily during storage at 32° F. If these apples are warmed to 65° F. during the period of exposure to 32° F., the subsequent rate of respiration at 32° F. is lower than before warming, and continues at a lower rate than for apples kept at 32° F. continuously.If the apples are moved to 38° F., without an intermediate treatment at 65° F., the rate of respiration is higher than for apples at 38° F. continuously, and this higher rate persists.If there is an intermediate wanning period at 65° F., the respiration of apples moved from 32° to 38° F. is of the same order as that for apples kept at 38° F. continuously.The respiratory quotient of apples at 32° F. or at 38° F., which is indicative of the type of respiratory activity, is typical for the temperature at which it is measured, and is not affected by the warming treatment. The effects of wanning on both the incidence of LTB and respiration are similar for apples stored in air and in 2% oxygen: 98% nitrogen. 相似文献
4.
AIM: To investigate the inhibitory effect of ground dragon on the expression of α-SMA and FN in the lung tissue with asthma. METHODS: The BALB/c mice were divided into four groups: control group (group A, n=20), asthmatic model group (group B, n=20), large-dose ground dragon treatment group (group C, n=20) and low-dose ground dragon treatment group (group D, n=20). To establish a mouse model of chronic asthma, we sensitized the mouse with 0.02% ovalbumin (OVA) by intraperitoneal injection, and stimulated the mice with 1% OVA by atomization. The treatment groups were given ground dragon before stimulation every time. After the last time of stimulation, the mice were subjected to laboratory tests. Inflammatory cells in bronchoalveolar lavage fluid were counted. Total level of IgE in serum was detected by ELISA. FN mRNA and α-SMA mRNA in the lung tissue were measured by RT-PCR and AlphaImager 2200 semi-quantitation analysis system. Expressions of FN and α-SMA were measured by the method of two-step immunohistochemistry and leica QWIN V3 analysis system. RESULTS: (1) Compared with those in group A, the expressions of α-SMA and FN in group B were significantly increased (P<0.01). Compared with group B, those in group C were significantly decreased (P<0.01), while those in group D were slightly decreased (P>0.05). (2) Compared with those in group A, the expression levels of α-SMA mRNA and FN mRNA in group B had a great increase (P<0.01). There was a notably decreases of α-SMA mRNA and FN mRNA levels in group C, compared with group B (P<0.01). However, α-SMA and FN mRNA level in group D was just a slightly decreased, compared with group B (P>0.05). CONCLUSION: The ground dragon inhibits α-SMA and FN expression in the lung tissue of mice with chronic asthma, indicating that ground dragon may inhibit airway remodeling in asthma through the inhibition of α-SMA and FN expressions. 相似文献
5.
B.J. Van Goor 《The Journal of Horticultural Science and Biotechnology》2013,88(4):347-364
In several commercial orchards the percentages of Ca and K in the leaves and fruit of the cultivar Cox’s Orange Pippin were determined at intervals during one or more years. The values for a series of untreated trees were continually compared with those of a comparable series of trees which were sprayed at about seven-day intervals with a Ca(NO3)2 solution. At the end of each experiment the fruit was placed in storage and later examined for the incidence of bitter pit.The Ca contents of leaves and fruit and the quotient (K+Mg)/Ca in the leaves of untreated trees showed only a moderate relationship with the percentage of bitter pit. The K content of the fruit was more closely correlated, however.The level above which much bitter pit was unlikely to occur was found to be approximately 7.5–8 mg Ca in a fruit of 140 g fresh weight.The total amount of Ca in the untreated fruit increased in a rather regular and comparable fashion in the various experiments. The rate of increase was somewhat higher during the first period of development, until the fruit reached about 20–40 g in weight, than during its subsequent growth.Spray treatments increased the Ca content of the leaves by 0.2–0.5% of the dry weight; a maximum of 4 mg Ca was added to the fruit, an increase of 47% on the basis of the amount already present. In general the Ca level increased progressively during the season with successive spray applications, and 9–15 sprays reduced bitter pit incidence to about 5%.The permeability of the fruit tissue to ions diminished as a result of the sprays, and it is suggested that an increase in cell membrane permeability may be involved in the development of the disease.For commercial orchards, more frequent sprays may need to be applied than are recommended at present to provide effective control of bitter pit. 相似文献
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WANG Jiancheng YING Zhenghe YU Yingrui LI Kaiben 《食用菌学报》2007,14(3):37-40
The effect of carbon and nitrogen sources, pH, temperature and substrate water content on the growth and vigor of Sparassis crispa mycelium was evaluated. Mycelial growth rates were highest when rice starch served as the carbon source and when peptone, yeast extract or soybean meal were adopted as the nitrogen source. The optimum pH and substrate water content values were 5.25 and 62.5% respectively. Mycelial growth was optimal at 20.2 ℃ and inhibited at 35 ℃, and temperatures of 40 ℃ and above killed the fungus. 相似文献
8.
Chestnut stands (orchards and coppices) are among the most typical elements of the southern European mountain landscape and
a protected habitat (9260 Castanea sativa woods) according to the European Union (Directive 92/43/EEC). As an anthropogenic landscape, they require specific measures
to address preservation or to guide their evolutionary trend. In the Northern Apennines, a landscape multiscalar-multitemporal
approach was adopted to highlight factors that have acted on the evolution of this habitat and which still might affect either
its preservation or its evolutionary dynamics. Using a diachronic GIS-approach, we analyzed old cadastral maps (drawn up 200 years
ago), and aerial photographs. Both the present distribution pattern of the woody species and the incidence of important chestnut
diseases were also surveyed. The factors explaining the current extent and species composition of the local chestnut forests
confirm their status as an anthropogenic habitat. The present landscape distribution of chestnut woods is heavily linked to
past human settlements. Chestnut blight and ink disease are more an indirect reason for past felling activities than an actual
direct cause of damage to trees, because of the hypovirulence spread and the limited incidence of the ink disease. Vegetation
dynamics of abandoned chestnut forests evolved only partly towards deciduous Beech and Hop Hornbeam stands, thus suggesting
both the possibility of a recovery of this cultivation and the need for new criteria for its management. 相似文献
9.
AIM:To investigate Nrf2 that regulates the expression of γ-glutamylcysteine synthetase (γ-GCS) in the inflammatory cells in bronchial asthma guinea pig bronchoalveolar lavage fluid (BALF). METHODS:Adult male guinea pigs were randomly divided into control group (group A), asthmatic group (group B) and dexamethasone group (group C). Asthmatic model was established by the method of ovalbumin challenge. MDA concentration in the lung tissue homogenate was detected. The total cell count and the proportion of inflammatory cells in BALF were measured. The methods of immunohistochemistry and in situ hybridization were used for detection of the protein and mRNA expressions of Nrf2, Bach1 and γ-GCS. RESULTS:(1) The proportion of eosinophils (EOS) in BALF and the MDA concentration of the lung tissue in group B were higher than those in group A and group C. (2) The result of in situ hybridization indicated that the A value of γ-GCS was the highest in group A compared to group B and group C, but the A value of Nrf2 and Bach1 in 3 groups has no statistical significance. (3) Immunohistochemistry indicated that the A value of γ-GCS in group B was lower than that in group A. The positive rate in cell nucleus of Nrf2 in group B was lower than that in group A and group C. The positive rate in cell nucleus of Bach1 in group B was higher than that in group A and group C. (4) The mRNA expression of γ-GCS (A value) showed positive correlation with the positive rate in cell nucleus of Nrf2 and negative correlation with the positive rate in cell nucleus. A negative correlation between the proportion of EOS in BALF of group B and the γ-GCS mRNA was observed. CONCLUSION:There is disequilibrium between oxidation and anti-oxidation in bronchial asthma guinea pig. Inflammatory reaction decreases the expression of γ-GCS in the inflammatory cells in bronchial asthma guinea pig. Dexamethasone regulates the nuclear translocation of Nrf2/Bach1 and increases the expression of γ-GCS. 相似文献
10.
The ongoing effects of the climate emergency and the recognition of the need to redesign cities to cope with future socio-environmental changes and increase the quality of life of urban inhabitants has pushed many cities to embrace the ‘re-naturalization’ of public space through Nature-based Solutions (NbS). Les Glòries park (Barcelona) is an ongoing massive urban transformation project that incorporates NbS as a multifunctional tool. It attempts to respond to complex environmental, social, and economic urban problems while at the same time mitigating and adapting to hazards, such as floods and droughts, both predicted to increase in the Mediterranean region due to climate change. While NbS are also expected to improve environmental quality, not much attention has been given to how those strategies would intersect with citizens’ expectations and visions of public space and the uneven socio-environmental relations they can enforce. Through a case of urban development of a new large green area in Barcelona, this article aims to discern different perceptions by the local community and planners on the socio-ecological role of NbS and the implications this has for sustainable urban transitions. In-depth interviews were held with 25 key stakeholders and users, complemented by the analysis of secondary data from the town council of Barcelona about the perception of the space by residents living in the surrounding neighborhoods of the park. The results of this paper show that perceptions around NbS are strongly divided into three groups: the believers, the opposers and the skepticals. These results contribute to nuance NbS, as they do not provide universal benefits, neither for nature nor for society, and that they are subject to urban tensions derived from inequities, conflicts, tradeoffs, and particularly from divided visions of what the ‘re-naturalization’ of cities should look like. 相似文献
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R. O. Sharples 《The Journal of Horticultural Science and Biotechnology》2013,88(3):224-235
Severe hand thinning of fruitlets on Cox’s Orange Pippin trees five weeks after full blossom led to a doubling of fruit weight by harvest. Although the rate of cell division was stimulated slightly, the larger fruit size was due mainly to an increased rate of cell enlargement.The respiration rate of whole fruits was slightly higher after thinning and the onset of the climacteric rise was advanced. Respiration per cell was correspondingly higher in the larger cells of the thinned fruit and respiration per unit protein was similar to that of fruit from unthinned trees.Potassium, magnesium, and phosphorus contents, expressed on a fresh weight basis, were higher in the cortical tissue of the thinned fruits. Calcium per unit fresh weight was unaffected by thinning and the ratio of calcium to cell surface remained relatively constant throughout development in both types of fruit.Senescent breakdown and bitter pit developed during storage only in the thinned fruit. Slight differences in the incidence of rotting and low temperature breakdown between the two types of fruit are attributed to the effects of maturity rather than of fruit size. 相似文献
13.
AIM: To explore the effect of 4-hydroxytomacifen (4-OHT) on MER-Syk(L) cellular localization and the function of Syk(L) on cell proliferation in breast cancer cells.METHODS: pcDNA3.1(+)-MER-Syk(L) vector was constructed and the cell line MDA-MB-231, which expressed MER-Syk(L) stably, was established. Western blotting and immunofluorescence techniques were used to detect localization of MER-Syk(L) fusion protein in MDA-MB-231 cells cultured with or without 4-OHT. MTT assay was used to explore the proliferation ability of MDA-MB-231 stable cells.RESULTS: (1) MER-Syk(L) fusion protein, not MER-Syk(S) and MER protein, translocated from cytoplasm to nucleus in the presence of 4-OHT. (2) Nuclear not cytoplasmic MER-Syk(L) fusion protein inhibited MDA-MB-231 stable cell growth. (3) With or without the treatment of 4-OHT, MER-Syk(S)and MER protein always located in cytoplasm and did not suppress cell growth.CONCLUSION: With 4-OHT, MER-Syk(L) fusion protein translocates to nucleus and inhibits cell growth. 相似文献
14.
Y. M. Lee H. J. Lee Y. S. Kim S. Y. Kang D. S. Kim J. B. Kim 《The Journal of Horticultural Science and Biotechnology》2016,91(2):109-116
Gamma-rays are an important mutagenic agent that can induce new, useful genetic variations in plants. However, γ-irradiation can also cause damage that negatively affects the use of such mutagens in plant breeding. The aim of the present study was to investigate the effects and damage caused by γ-radiation in a Cymbidium hybrid, RB001. The relative growth rate of protocorm-like bodies (PLBs) was reduced by 50% at a γ-ray dose of approximately 40 Gy. Malondialdehyde concentrations increased significantly with increasing radiation dose. However, almost no difference was observed between untreated control PLBs and PLBs treated with 200 Gy 8 weeks after γ-irradiation. The activities of several antioxidant defence enzymes increased gradually with increasing γ-ray dose, 24 h after irradiation. These enzymes showed different responses between 1 and 4 weeks, but no difference 8 weeks after irradiation. The ‘comet assay’ and flow cytometry were performed. Clear differences in radiation-induced damage were observed between control and 200 Gy-treated PLBs at 24 h. However, PLBs had a tendency to recover from 4 weeks after irradiation, and the integrity of their DNA was similar in samples treated with 10–200 Gy. These results indicated that γ-rays caused little DNA damage and the plants could recover. This demonstrated the feasibility of using physiological responses, the ‘comet assay’, and flow cytometry to detect DNA damage after γ-irradiation. 相似文献
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Alexandra Wagstaffe H. Nick Battey 《The Journal of Horticultural Science and Biotechnology》2013,88(6):1086-1092
SummaryTemperature regimes that induce and ameliorate cropping troughs (“thermodormancy”) were evaluated over two seasons for the everbearing strawberry ‘Everest’. When plants were exposed to 26°C for 5, 10, 20 or 30 d in July, heat-induced troughs in cropping were observed in August. An important discovery was that cool (13°C) night temperatures ameliorated the severity of thermodormancy. In this study, thermodormancy appeared to be due principally to flower abortion post-anthesis, as large numbers of flowers emerged in mid-July, during the high temperature treatments, but went on to produce low fruit numbers in mid-August. Flower initiation itself (monitored by crown dissection) was not reduced by high temperatures. The observation that night-time temperature is critical for thermodormancy has significance for commercial production, in which protected cropping tends to increase average temperatures throughout the season, and venting tends to focus on day-time temperatures. 相似文献
17.
Bryan C. Pijanowski Louis R. Iverson C. Ashton Drew Henry N. N. Bulley Jeanine M. Rhemtulla Michael C. Wimberly Annett Bartsch Jian Peng 《Landscape Ecology》2010,25(1):5-16
We argue for the landscape ecology community to adopt the study of poverty and the ecology of landscapes as a Grand Challenge
Topic. We present five areas of possible research foci that we believe that landscape ecologists can join with other social
and environmental scientists to increase scientific understanding of this pressing issue: (1) scale and poverty; (2) landscape
structure and human well-being; (3) social and ecological processes linked to spatial patterns in landscapes; (4) conservation
and poverty, and (5) applying the landscape ecologist’s toolkit. A brief set of recommendations for landscape ecologists is
also presented. These include the need to utilize broad frameworks that integrate social and ecological variables, build capacity
to do this kind of work through the development of strong collaborations of researchers in developed and developing countries,
create databases in international locations where extreme poverty exists, and create a new generation of researchers capable
of addressing this pressing social and environmental issue. 相似文献
18.
B. N. Krath F. D. Eriksen B. H. Pedersen L. J. W. J. Gilissen W. E. Van De Weg L. O. Dragsted 《The Journal of Horticultural Science and Biotechnology》2013,88(6):52-57
SummaryMany people who are allergic to birch pollen are also allergic to apple fruit, due to cross- allergenicity. Since apples are the most extensively consumed fruit in Europe, it is highly relevant to develop a hypo-allergenic apple.Apples with significantly reduced levels of the allergen, Mal d 1, may allow many apple allergics to eat them without an allergic reaction. We are currently collaborating to develop a hypo-allergenic apple within the European Integrated Research Project, ISAFRUIT (www.isafruit.org). Hypo-allergenic apple plants (Malus × domestica Borkh., ‘Elstar’) with decreased levels of Mal d 1 mRNA were produced by RNA interference (RNAi) technology. Ten genetically modified (GM) apple lines were selected. In vitro plantlets were first transferred to a greenhouse, then grafted onto wild-type M.9 rootstock to promote the development of fruit-producing trees. Levels of Mal d 1 gene silencing were measured repeatedly by quantitative real-time PCR. Compared to leaf samples from wild-type ‘Elstar’, two GM lines showed modest levels of gene silencing (up to 250-fold), whereas the other eight GM lines were significantly silenced (up to10,000-fold) in Mal d 1 gene expression. These levels of silencing were unaffected by grafting, and have been stable over more than 3 years, and throughout all developmental stages. 相似文献
19.
In Central Europe vast wetland areas have been converted into agricultural land over the past few centuries. Long-term spatially
explicit reconstructions of wetland cover changes at regional scale are rare but such information is vital for setting appropriate
wetland conservation and restoration goals. In this study wetland cover change over the past 150 years was analyzed for the
Canton Zurich (Switzerland) using information from historical and current topographical maps. Mapping instructions changed
significantly over time, i.e., wetlands were mapped more conservatively on older maps. Therefore a technique was developed
to account for changes in mapping instructions and to reconstruct a series of comparable maps spanning 1850–2000. Wetland
cover dramatically decreased from 13,759 ha in 1850 (more than 8% of the total study area) to 1,233 ha in 2000 (less than
1%). Largest loss is observed for the first half of the twentieth century when more than 50% of the total wetland loss occurred.
In 1850, almost all wetland patches were connected in two large networks defined by a 500 m buffer around all wetland patches
to account for typical dispersal distances of wetland animals. Despite extensive wetland loss, this networks remained largely
intact until 1950, but then collapsed into many medium and small networks consisting of only few wetland patches. In addition
to the direct loss of wetland habitats increased habitat fragmentation is limiting metapopulation dynamics and hindering genetic
exchange between populations. Amphibians and other wetland animals are particularly prone to habitat fragmentation because
of their limited migration abilities. This may lead to time-delayed extinction in the future because current species occurrence
might rather reflect historical than current wetland cover and habitat configuration. Future restoration efforts should focus
on reestablishing connectivity between remaining smaller wetland networks. 相似文献
20.
Two ecological models have been put forward to explain the dynamics of fire-promoting and fire-sensitive vegetation in southwest
Tasmania: the alternative stable states model of Jackson (in Proc Ecol Soc Aust 3:9–16, 1968) and the sharpening switch model of Mount (in Search 10:180–186, 1979). Assessing the efficacy of these models requires high resolution spatio-temporal data on whether vegetation patterns are
stable or dynamic across landscapes. We analysed ortho-rectified sequences of aerial photography and satellite imagery from
1948, 1988 and 2010 to detect decadal scale changes in forest and non-forest vegetation cover in southwest Tasmania. There
was negligible change from forest to non-forest (<0.05%) and only a modest change from non-forest to forest over the study
period. Forest cover increased by 4.1% between 1948 and 1988, apparently due to the recovery of forest vegetation following
stand-replacing fire prior to 1948. Forest cover increased by 0.8% between 1988 and 2010, reflecting the limited ability of
forest to invade treeless areas. The two models include interactions between vegetation, fire and soil, which we investigated
by analysing the chemical (phosphorus, nitrogen) and physical properties (clay, silt) of 128 soil samples collected across
34 forest–non-forest boundaries. Phosphorus in the upper horizon was typically lower in non-forest vegetation compared to
forest vegetation, which is consistent with proposed fire–vegetation–soil feedbacks. Mineral horizons were dominated by sand,
with low levels of clay under all vegetation types. Available field evidence lends support to the Jackson (1968) alternative stable states model as the most suitable model of vegetation dynamics on nutrient poor substrates in southwest
Tasmania although modifications of the timeframes for transitions toward rainforest are required. 相似文献