The role of tephra covers on soil moisture conservation at Haleakala's crater (Maui, Hawai'i)

The influence of tephra covers on soil water was studied in Haleakala (Maui, Hawai'i) during two summers; eight sites with tephra layers and silverswords (Argyroxiphium sandwicense DC.) were sampled at 2415–2755 m. At each site, eight paired-sample sets were obtained in bare soils and under adjacent tephra, at three depths. Tephra were sharply separated from underlying soils and showed prominent vertical stratification. Tephra clast size-distribution was assessed by photosieving and on interstitial-gravel samples; stones included 45.6% cobbles, 29.4% pebbles, and 25% blocks.Moisture content increased with depth in both positions, but soils below tephra had more water at all depths than exposed areas. Surface soils beneath tephra contained 83% more water than bare ground. Soils at 5–10 cm had  106% greater moisture under rocks, but only  70% at 10–15 cm. Differences between plots were statistically significant ( p < 0.001) for surface soils, but less pronounced for subsoils. Soils above 2650 m had greater water content than at lower elevations, and moisture disparity between sample pairs increased with altitude.All soils were coarse, with  20% gravel and  94% sand; most fine material (≤ 0.063 mm) was silt, as clay content was negligible. Organic-matter percentage was low (1.65%). Bulk density and porosity were associated with moisture variation both in tephra-insulated and bare soils; 80% of field moisture was statistically (p < 0.001) accounted for by pore space. Air and soil temperatures were recorded at three sites during  one-week periods prior to moisture sampling. Tephra substantially decreased soil maxima and daily thermal amplitude in underlying soils, but did not noticeably affect nightly minima. Thin (5–6 cm) tephra layers were nearly as effective as thicker (9–15 cm) deposits in depressing soil maxima. Possible water-conservation mechanisms under tephra include: decreased evaporation due to ground shielding and lower maxima; reduced capillary flow; greater infiltration depth; nocturnal dew condensation; and fog interception by blocks.     

Response of silicate weathering to monsoon changes on the Chinese Loess Plateau

Eolian deposits of loess on the Chinese Loess Plateau are investigated to decipher the influence of sequentially changed monsoon climate on silicate weathering process. Detailed hydrochloric acid dissolution experiments are applied to establish a sensitive proxy for silicate weathering. Combined elemental and mineralogical studies show that the minerals which are susceptible to incipient chemical weathering can be totally dissolved in hot hydrochloric acid (80°C, 3mol/L) after 4h, while other stable minerals are nearly unaffected. Thus, the hydrochloric acid dissolvable fractions (ADF) of loess and paleosol are more sensitive than bulk samples in the weathering reaction. Since Mg is tend to be leached out from the ADF while Al is conserved during the incongruent weathering, Mg/Al ratio of the ADF (Mg/Al_ADF) could indicate the weathering intensities of the Mg-bearing minerals, mainly chlorite. The Mg/Al_ADF of the modern soils on the Chinese Loess Plateau is closely correlated to the local precipitation amounts, suggests that the intensity of summer monsoon is a key factor in the weathering of Mg-silicate. The silicate weathering intensity of the loess-paleosol deposits of past 130Kyrs shows strong procession cycle of 23Kyrs coupled by glacial-interglacial variation. The 23Krys cycles are coherent to the variations of the intensity of summer monsoon, while the 100Kyrs glacial-interglacial variation may relates to the changes in winter monsoon. Phased intensification of East Asian summer monsoon, and thus enhanced silicate weathering and atmosphere CO₂ drawdown, in response to the uplift of Tibetan Plateau, may be another mechanism relating the late Cenozoic tectonics to global cooling.     

Surface energy balance closure by the eddy-covariance method above three boreal forest stands and implications for the measurement of the CO2 flux

Closure of the surface energy balance provides an objective criterion for evaluating eddy-covariance (EC) flux measurements. This study analyses 5 years of EC carbon dioxide, water vapor, and sensible heat flux measurements from three mature boreal forest stands in central Saskatchewan, Canada. The EC sensible and latent heat fluxes, H and λE, underestimated the surface available energy by 11% (aspen), 15% (black spruce), and 14% (jack pine). At all sites, the energy-closure fraction CF responded similarly to the friction velocity u_*, atmospheric stability, and time of day. At night, CF increased from 0.3 at very low-u_* to an asymptotic maximum of 0.9 at u_* above 0.35 m s⁻¹. During unstable-daytime periods, CF varied linearly from 0.7 at low-u_* to 1.0 at high-u_*. The energy imbalance pattern was similar among sites and may be characteristic of the continental, boreal forest.EC measurements of net ecosystem exchange F_NEE have no objective, diagnostic parameter that is equivalent to CF. We therefore derived an analogous F_NEE “closure fraction” CF^NEE by normalizing measured F_NEE against estimates from an empirical model that was tuned to the high-u_* data. CF and CF^NEE responded similarly to u_*, atmospheric stability, and time of day. We discuss two implications for EC flux data post-processing. The results uphold the common practice of rejecting EC measurements during low-u_* periods. They also lend support to the application of energy-closure adjustments to H, λE, and F_NEE.     

Evaluation of non-invasive genetic sampling methods for estimating tiger population size

There is often a conservation need to estimate population abundances of elusive, low-density, wide-ranging carnivore species. Because of logistical constraints, investigators often employ non-invasive ‘captures’ that may involve ‘genetic’ or ‘photographic’ sampling in such cases. Established capture–recapture (CR) methods offer a powerful analytical tool for such data. In this paper, we developed a rigorous combination of captive, laboratory and field-based protocols for identifying individual tigers (Panthera tigris) from fecal DNA. We explored trade-offs between numbers of microsatellite loci used for reliable individual identifications and the need for higher capture rates for robust analyses. Our field surveys of scats were also specifically designed for CR analyses, enabling us to test for population closure, estimate capture probabilities and tiger abundance. Consequently, we could compare genetic capture estimates to results of a ‘photographic capture’ study of tigers at the same site. The estimates using the heterogeneity model (M_h-Jackknife) for fecal DNA survey were [M_t+1 = 26; and ()=66 (12.98)] in close agreement with those from the photographic survey [(M_t+1 = 29; and () = 66 (13.8)]. Our results revealed that designing field surveys of scats explicitly for CR data analyses generate reliable estimates of capture probability and abundance for elusive, low density species such as tigers. The study also highlights the importance of rigorous field survey and laboratory protocols for reliable abundance estimation in contexts where other approaches such as camera-trapping or physical tagging of animals may not be practical options.     

Oxygen exchange with water alters the oxygen isotopic signature of nitrate in soil ecosystems

Combined oxygen (O) and nitrogen (N) stable isotope analyses are commonly used in the source determination of nitrate . The source and fate of are studied based on distinct O and N isotopic signatures (δ¹⁸O and δ¹⁵N) of various sources and isotopic effects during transformation processes, which differ between sources like fertilizer, atmospheric deposition, and microbial production (nitrification). Isotopic fractionation during production and consumption of further affects the δ¹⁸O and δ¹⁵N signal. Regarding the δ¹⁸O in particular, biochemical O exchange between O from and H₂O is implicitly assumed not to affect the δ¹⁸O signature of . This study aimed to test this assumption in soil-based systems. In a short (24 h) incubation experiment, soils were treated with artificially ¹⁸O and ¹⁵N enriched . Production of from nitrification during the incubation would affect both the ¹⁸O and the ¹⁵N enrichment. Oxygen exchange could therefore be studied by examining the change in ¹⁸O relative to the ¹⁵N. In two out of the three soils, we found that the imposed ¹⁸O enrichment of the declined relatively more than the imposed enrichment. This implies that O exchange indeed affected the O isotopic signature of , which has important implications for source determination studies. We suggest that O exchange between and H₂O should be taken into consideration when interpreting the O isotopic signature to study the origin and fate of in ecosystems.     

Direction-dependent behaviour of hydraulic and mechanical properties in structured soils under conventional and conservation tillage

The development of soil structure units with defined forms and dimensions (e.g. platy by soil compaction or prismatic up to subangular-blocky by swelling–shrinkage processes) can lead to direction-dependent behaviour of mechanical and hydraulic properties. However, little research has investigated direction-dependent behaviour directly. Undisturbed samples were collected at different horizons and orientations (vertical and horizontal) of Stagnic Luvisols derived from glacial till (Weichselian moraine region in Northern Germany). A direct shear test determined the cohesion (c) and the angle of internal friction (φ). The water retention curve (WRC), the saturated hydraulic conductivity (k_s) and the air permeability (k_a) were also measured. The air-filled porosity (_a) was determined and pore continuity indices (N) and blocked porosities (_b) were derived from the relationship between k_a and _a.Although the pore volume as a scalar is isotrop, the saturated hydraulic conductivity and air permeability can be anisotropic. In the seedbed (SB) and plough pan (PP) of conventionally managed soils the effective porosity is non-direction-dependent, however, differences in k_s as a function of sampling direction can reach one order of magnitude in PP (k_sh > k_sv). The shear strength parameters do not present a significant anisotropy, although, a pronounced spatial orientation of soil aggregates (e.g. induced by soil compaction in a plough pan) lead to direction-dependent shear strength (by σ_n: 10 kPa, σ_tv: 12 kPa and σ_th: 19 kPa). This behaviour was especially observed in pore continuity indices (e.g. vertical and horizontal oriented aggregates observed in Bvg and PP presented _bv < _bh and _bv > _bh, respectively) showing that the identification of soil structure can be used as the first parameter to estimate if hydraulic properties present a direction-dependent behaviour at the scale of the soil horizon, which is relevant in modelling transport processes.     

Microhabitats of Collembola (Insecta: Entognatha) in beech and spruce forests and their influence on biodiversity

Collembolan communities were studied in 41 microhabitats in beech and spruce forests of south ( ofín and umava) and central (Jevany) Bohemia. The communities of Collembola were analysed using TWINSPAN and CANOCO programs. The aim of this study was to establish differences between patch (microhatitat) communities and the main forest community in spruce and beech forests, the differences between both types of forests and among different regions of Bohemia. Further questions were: is there a difference in microhabitat communities during secondary forest succession? do some species live exclusively in one or few microhabitats? and does microhabitat diversity influence the biodiversity in forest soils? Material comprising  25 590 specimens of Collembola belonging to 142 species was analysed. Highly significant differences were determined between microhabitat communities in beech and spruce forests, as well as among forests in different regions of Bohemia. Significant differences were also found among microhabitats in forests of different ages. Also, some microhabitat communities of Collembola, e.g. moss on boulders, were significantly different from their main forest community. Certain collembolan species existed exclusively in one or two microhabitats. Patches therefore influenced substantially biodiversity in these forest soils.     

Photosynthetic light use efficiency of three biomes across an east–west continental-scale transect in Canada

Light use efficiency (LUE) is used widely in scaling and modeling contexts. However, the variation and biophysical controls on LUE remain poorly documented. Networks of eddy covariance (EC) towers offer an opportunity to quantify _g, the ratio of P, gross primary productivity, to Q_a, absorbed photosynthetically active radiation (PAR), across climate zones and vegetation types. Using data from the Fluxnet Canada Research Network (n = 24 sites) in 2004, we examined the relationship between daily and yearly _g, driving variables, and site characteristics on a site-specific and plant functional type (PFT) basis using tree regression and linear regression. Data were available for three biomes: grassland, forest, and wetland. Yearly _g values ranged from 0.1 to 3.6 g C MJ⁻¹ Q_a overall. Daily _g was highest in the grassland (daily median ± interquartile range: 3.68 ± 1.98 g C MJ⁻¹ Q_a), intermediate in the forested biome (0.84 ± 0.82 g C MJ⁻¹ Q_a), and lowest for the wetlands (0.65 ± 0.54 g C MJ⁻¹ Q_a). The most important biophysical controls were light and temperature, to the exclusion of water-related variables: a homogeneity of slopes model explained c. 75% of the variation in daily _g. For a subset of sites with diffuse PAR data, the ratio of diffuse to total PAR, a proxy for cloudiness, was a key predictor. On the yearly time scale, _g was related to leaf area index and mean annual temperature. Aggregating to PFTs did not show functional convergence within any PFT except for the three wetland sites and the Picea mariana toposequence at the daily time step, and when using the Köppen climate classification on a yearly time step. The general lack of conservative daily _g behavior within PFTs suggests that PFT-based parameterizations are inappropriate, especially when applied on shorter temporal scales.     

Evaluation of land surface radiation balance derived from moderate resolution imaging spectroradiometer (MODIS) over complex terrain and heterogeneous landscape on clear sky days

Many studies on land surface radiation balances have relied on geostationary satellites. These satellites have provided data with high temporal resolution (less than 3 h); however, the spatial resolution was too coarse (20–250 km scale) to investigate local-scale land surface radiation balances. Moderate resolution imaging spectroradiometer (MODIS) – onboard both the Terra and Aqua satellites – yields a tradeoff with regard to this problem by providing higher spatial resolution (1 km scale) and sensing all over the earth nearly twice a day during daytime; this provides a potential tool for the periodical monitoring of the land surface energy balance. The reliability of MODIS-derived estimates is, however, affected by the presence of multiple error sources, such as those related to heterogeneous land cover and complex topography. In this study, we have used atmospheric (5 and 10 km scale) and land (1 km scale) products obtained from both the Terra and Aqua MODIS devices as inputs in order to estimate the radiation components (1 km scale) under clear daytime conditions over a heterogeneous farmland area and a rugged deciduous forest in the Korea Flux Network (KoFlux). The reliability of these estimates and the associated errors were evaluated by comparing against field measurements taken for 41 and 26 clear days with regard to the farmland and forest sites, respectively. Solar radiation was successfully retrieved with a root mean square error (RMSE) of 20 W m⁻² for both the Terra and Aqua devices over the flat farmland site, whereas the rugged forest site exhibited corresponding values of 40 and 65 W m⁻² RMSE values with consistent positive biases (presumably caused by topographic effects). The RMSE values of the downward longwave radiation were 20 W m⁻² for both the Terra and Aqua devices for both these sites. The sensitivities of the upward components of the shortwave and longwave radiations varied with the RMSE values to the scale of the spatial heterogeneity of both the sites. Consequently, the RMSE values of the net radiation ranged from 33 to 61 W m⁻² for both the devices at both the sites. Our results suggest that the scales of the patch mosaics within the landscapes need to be quantified for proper retrieval of the MODIS-derived radiation products. More extensive validation efforts are required to identify and account for major error sources across diverse land surface conditions.     

Alteration of secondary minerals along a time series in young alkaline soils derived from carbonatic wastes of soda production

Industrial activities result in increasing amounts of technical substrates being deposited in landfills. These substrates are subject to weathering and pedogenic processes. We studied the chemical and mineralogical transformations on naturally weathered waste deposits of soda industry. Four sites differing in age (15, 19, 57, and 70 years) and derived from carbonatic slurry (mainly CaO^.H₂O, CaCO₃, NaCl) were selected. The formed soils, calcareous spolic Regosols, are weakly to strongly alkaline with pH values ranging from 8 to 12. Within 15 years, the substrate's initial pH of 12 drops rapidly in the topsoil due to the reaction of dissolved Ca either with CO₂ from the atmosphere or evolved by microbial respiration and finally stabilizes at around 8.1. All soils show high electrical conductivity, up to 12.3 mS cm^− 1 at the youngest site. The electrical conductivity strongly decreases within 70 years of weathering due to leaching processes and the formation of less soluble secondary minerals. The content of organic C in the studied soils ranges from 2.4 to 70.8 g kg^− 1 and stocks increase with site age. Soil structure and soil color change distinctly. The binding of CO₂ results in large amounts of carbonate, increasing with time. Seventy years after deposition, calcite [CaCO₃] dominates the topsoil (0–30 cm depth), comprising about 80% of the soil material. The mineral composition was characterized by X-ray diffraction. Besides calcite, we found different quantities and different distributions of the less common minerals ettringite [Ca₆(Al(OH)₆)₂(SO₄)₃  26H₂O], thaumasite [Ca₆(Si(OH)₆)₂(CO₃)₂(SO₄)₂  24H₂O], hydrocalumite [Ca₂Al(OH)₇  2H₂O] and hydrotalcite [Mg₆Al₂(CO₃)(OH)₁₆  4H₂O]. Formation and alteration of these minerals are basically influenced by changes in the soil pH. With progressing weathering neither thaumasite nor ettringite are stable due to the non-favorable soil reaction (pH  8.1). In contrast, hydrocalumite and hydrotalcite exist in all investigated soils. They are stable also under weakly alkaline conditions and thus may exist in all carbonatic soils. Results indicate a surprisingly rapid soil development driven by the highly dynamic formation and alteration of minerals in carbonatic substrates under alkaline conditions.     

Constraining the conditions conducive to dissimilatory nitrate reduction to ammonium in temperate arable soils

Here we offer the first assessment of conditions conducive to dissimilatory nitrate reduction to ammonium (DNRA) in temperate arable soils, through an examination of the potential for this process to occur in a range of soils of contrasting characteristics. NH₄¹⁵NO₃ (6.2 g N m⁻², 25 atom % excess ¹⁵N) was applied, and recovery of ¹⁵N in the pool taken as indicative of occurrence of DNRA. Up to 5% of applied ¹⁵N was recovered in the pool 2 d after addition of N, glucose (44.6 g C m⁻²) and l-cysteine (7.7 g m⁻², 0.9 g N m⁻², 2.3 g C m⁻²). concentrations were positively correlated with soil pH, ratio, bulk density, sand content and concentration, but negatively correlated with soil C and organic N content. Our results demonstrate the potential for DNRA to contribute to N cycling in temperate arable soils, but its detection and significance is likely to depend on the provision of a low molecular weight C source.     

Ammonium production during microbial nitrate removal in soil microcosms from a developing marsh estuary

Rapid development and urbanization in the South Carolina (SC) coastal plain may introduce significant nutrients to adjacent tidal creeks and salt marsh estuaries, and threaten estuarine water quality. Microbial denitrification in estuarine soils plays an important role in removing excessive nitrate in coastal waters. Relative contributions of denitrification and ammonium production during nitrate reduction via dissimilatory nitrate reduction to ammonium (DNRA) and soil mineralization determine whether N is lost from the system or retained as ammonium . The objectives of this study were to compare background, short-term and long-term potential denitrification ( and glucose added) rates, and production during microbial conversion in a developing marsh estuary, SC (USA). Denitrification rates were measured using the acetylene block technique in an undeveloped fresh water site (T1W), an undeveloped Spartina marsh (Grave's Dock, GD), and a Spartina marsh at a golf course resort (Chechessee marsh, C3M). Background denitrification with no added was primarily controlled by concentration in soils and surface water. Adding glucose did not enhance either short-term or long-term potential denitrification rates in GD marsh soils. production during microbial removal was significant at both marsh sites, and N-mass balance based on N₂O and production suggested a significant contribution of from sources other than DNRA. DNRA was estimated to account for approximately 16.3% and 0% of total added removal in GD surface (0-10 cm) and subsurface (30-40 cm) soils, and 1.9 and 23.2% in C3M surface and subsurface soils. Excessive generation from processes other than DNRA may be attributed to stimulated mineralization, and this stimulation was estimated to enhance soil ammonification by 0.5∼4 times compared to background generation with no added. Our results suggest that although the marsh soils displayed high potential of removal via denitrification, the produced via a combination of DNRA and enhanced mineralization may allow to accumulate and be transported to coastal waters.     

Effect of earthworm cast formation on the stabilization of organic matter in fine soil fractions

To study the effects of earthworm casting on organic matter dynamics, control soil and casts were added as a surface layer (Horizon I) to perspex cylinders containing a ‘base’ soil depleted in organic C (Horizon II). Three treatments with different Horizon I were used; a control containing uningested soil and oak litter (Quercus petraea (Mattuschka) Lieblein), cast derived from the same substrates, and a third (Ew+cast) where cast as well as endogeic (Aporrectodea caliginosa Savigny, Allolobophora chlorotica Savigny) and anecic (Lumbricus terrestris L.) earthworms were included. These were monitored over a 2 year period. Moisture fluctuations were reduced and higher amounts of organic matter and C were present in Horizon I with the cast treatment after 2 years. In addition, the proportion of clay C (and to a lesser extent light fraction) in Horizon I decreased significantly in the control and cast treatments while there was a corresponding increase in the proportions of silt C. Overall, earthworm activity had a limited effect on C distribution in the particle size fractions studied, though the dynamics of organic matter/particle associations may require more than 2 years before clear patterns emerge.     

Tillage effect on C stocks of a clayey Oxisol under a soybean-based crop rotation in the Brazilian Cerrado region

A large area (180 Mha) of central Brazil is occupied by a savanna biome known as the Cerrado. Annual rainfall in this region varies from 1200 to 2000 mm, although there is a long (5 month) dry season with almost no rain. This region is regarded by Brazilians as their agricultural frontier and there is a steady growth in the area dedicated to permanent cropping in the region, which today is estimated to occupy 14 Mha. Owing to the dearth of long-term experiments, the impact of continuous cropping on soil carbon stocks remains unclear. The objective of this study was to evaluate the effects of different tillage systems (zero till (ZT) and conventional tillage (CT)) on the change in soil carbon stocks over a 20-year period of the same crop sequence compared to that under a neighbouring area of native vegetation (NV). Only approximately 10 Mg ha⁻¹ of soil carbon in the 0–100 cm depth interval was lost under continuous ZT. However, under CT systems losses were greater (up to 30 Mg C ha⁻¹) when the mouldboard plough was used and/or tillage was performed twice a year. We did not have access to instrumentation to accurately assess soil charcoal but the C/N data and peroxide and dichromate oxidative techniques suggested that 40% of soil C was in this form. The ¹³C natural abundance of soil profiles indicated that residues of crops (maize) and the spontaneous annual fallow of Brachiaria spp. resulted in integration of significant C₄ residues to a depth of at least 40 cm. It would appear that zero tillage, which is already widely adopted in the Cerrado region of Brazil, will have only a small negative long-term impact on soil C stocks, but ploughing, especially more than once a year, will lead to considerably larger soil C losses.     

Using an unplanned experiment to evaluate the effects of hatcheries and environmental variation on threatened populations of wild salmon

Efforts to conserve depleted populations of Pacific salmon (Oncorhynchus spp.) often rely on hatchery programs to offset losses of fish from natural and anthropogenic causes, but their use has been contentious. We examined the impact of a large-scale reduction in hatchery stocking on 15 populations of wild coho salmon along the coast of Oregon (USA). Our analyses highlight four critical factors influencing the productivity of these populations: (1) negative density-dependent effects of hatchery-origin spawners were 5 times greater than those of wild spawners; (2) the productivity of wild salmon decreased as releases of hatchery juveniles increased; (3) salmon production was positively related to an index of freshwater habitat quality; and (4) ocean conditions strongly affect productivity at large spatial scales, potentially masking more localized drivers. These results suggest that hatchery programs’ unintended negative effects on wild salmon populations, and their role in salmon recovery, should be considered in the context of other ecological drivers.     

Nitrite production by Nitrosomonas europaea and Nitrosospira sp. AV in soils at different solution concentrations of ammonium

Although it remains unclear why NH₃-oxidizing bacteria (AOB) of the genus Nitrosospira dominate soil environments, and why Nitrosomonas spp. are less common, virtually no studies have compared their behavior in soil. In this study, the NH₃ oxidation rates of Nitrosomonas europaea (ATCC 19718) and Nitrosospira sp. AV were compared in three differently textured soils containing a range of extractable contents (2-11 μg soil). Soils were adjusted to pH 7.0-7.4 with CaCO₃ and sterilized with γ-radiation. Cell suspensions of each bacterium were inoculated into the soils to bring them to two-third of water-holding capacity and cell densities ∼2.5×10⁶ g⁻¹ soil. In virtually all cases, rates of production for both N. europaea and Nitrosospira sp. AV were linear over 48 h, and represented between 13 and 75%, respectively, of the maximum rates achieved in soil-free bacterial suspensions. Soil solution concentrations that supported these rates ranged between 0.2 and 1.5 mM. Addition of 21-36 μg soil raised soil solution levels to 1.8-2.5 mM and stimulated production to a greater extent in N. europaea (3.3-6.6-fold) than in Nitrosospira sp. AV (1-2.1-fold). Maximum rates of production were obtained by raising soil solution levels to 3-4 mM with a supplement of ∼80-90 μg soil. K_s values in soil for Nitrosospira sp. AV and N. europaea were estimated as 0.14 and 1.9 mM , respectively, and estimates of V_max were about 3.5-times higher for N. europaea (0.007 pmol h⁻¹ cell⁻¹) than for Nitrosospira sp. AV (0.002 pmol h⁻¹ cell⁻¹). The cell density of N. europaea increased in sterile Steiwer soil independent of supplemental . In the case of treatments receiving supplemental , growth yields of N. europaea calculated from either produced or consumed were similar to those reported in literature (3.5×10⁶-6×10⁶ cells μmol⁻¹). A higher growth yield was measured in the case of zero added (2.7×10⁷ cells μmol⁻¹), indicating that use of organic carbon compounds might have occurred and resulted in some energy sparing. Our results suggest that Nitrosospira spp. with a K_s similar to Nitrosospira sp. AV may have an advantage for survival in soil environments where soil solution levels are less than 1 mM. However, it is apparent that AOB like N. europaea are poised to take advantages of modest increases in extractable that raise soil solution levels to about 2.0-2.5 mM.     

Plant nitrate use in deciduous woodland: the relationship between leaf N, N natural abundance of forbs and soil N mineralisation

Our aim was to study whether the in situ natural abundance ¹⁵N (δ¹⁵N)-values and N concentration of understory plants were correlated with the form and amount of mineral N available in the soil. Also to determine whether such differences were related to earlier demonstrations of differences in biomass increase in the same species exposed to nutrient solutions with both and or to alone. Several studies show that the δ¹⁵N of in soil solution generally is isotopically lighter than the δ¹⁵N of due to fractionation during nitrification. Hence, it is reasonable to assume that plant species benefiting from in ecosystems without significant leaching or denitrification have lower δ¹⁵N-values in their tissues than species growing equally well, or better, on We studied the δ¹⁵N of six understory species in oak woodlands in southern Sweden at 12 sites which varied fivefold in potential net N mineralisation rate The species decreased in benefit from in the following order: Geum urbanum, Aegopodium podagraria, Milium effusum, Convallaria majalis, Deschampsia flexuosa and Poa nemoralis. Four or five species demonstrated a negative correlation between and leaf δ¹⁵N and a positive correlation between and leaf N concentration. In wide contrast, only D. flexuosa, which grows on soils with little nitrification, showed a positive correlation between and the leaf N concentration and δ¹⁵N-value. Furthermore, δ¹⁵N of plants from the field and previously obtained indices of hydroponic growth on relative to were closely correlated at the species level. We conclude that δ¹⁵N may serve as a comparative index of uptake of among understory species, preferably in combination with other indices of N availability. The use of δ¹⁵N needs careful consideration of known restrictions of method, soils and plants.     

Nitrate uptake by Eriophorum vaginatum controls N2O production in a restored peatland

The clear dependence of N₂O production through denitrification on available nitrate in soil has been shown in many studies. Since N availability similarly limits the growth of plants, the resource competition with vegetation limits the activity of denitrifying microbes and may consequently moderate the N₂O emissions from peatlands. We used uptake by Eriophorum vaginatum L. as a vegetation competition factor for microbes. The species was selected for the experiment because it has high nutrient use efficiency in low-nutrient conditions and high nutrient uptake efficiency in luxuriant nutrient conditions. We measured gaseous N flux as N₂O (end product of denitrifier activity) in a restored peatland in central Finland with acetylene inhibition technique over a growing season from sample plots with varying addition levels and E. vaginatum cover. The resource competition effects were analysed with a model that used exponential decay dependence of N₂O flux on the leaf area of E. vaginatum, and saturating response of N₂O flux to addition level. The model explained the variation in N₂O fluxes well (R²=0.86). The model simulation showed that the increasing nutrient uptake of E. vaginatum decreased the N₂O fluxes exponentially. Simultaneously, denitrification appeared to saturate even in conditions with high availability of and low level of competition by vegetation. Thus, E. vaginatum is an effective competitor for in sedge-dominated peatlands that controls the availability of for denitrification, and consequently moderates the N₂O emissions from peatlands.     

The impacts of inorganic nitrogen application on mineralization of C-labelled maize and glucose, and on priming effect in saline alkaline soil

Organic matter dynamics and nutrient availability in saline alkaline soil of the former lake Texcoco will determine the success of a planned reforestation program. Uniformly labelled ¹⁴C-maize (MAI-treatment) and glucose (GLU-treatment) with or without 200 mg  kg⁻¹ soil (MAI-N treatment and GLU-N treatment, respectively) were added to soils with electrolytic conductivity (EC) 56 dS m⁻¹ (soil A) and 12 dS m⁻¹ (soil B) to investigate the importance of N availability on decomposition of organic material. Production of CO₂ and and inorganic N dynamics were monitored. The amount of ¹⁴C-glucose mineralized increased 1.8-times in the soil A, but had no effect in the soil B when 200 mg  kg⁻¹ soil was added, while the amount of ¹⁴C-maize mineralized increased 1.7 and 1.3-times when 200  kg⁻¹ soil was added in the soils A and B, respectively. Application of increased priming effect 3.7-times in the MAI-treatment of the soil A and 3.4-times in the GLU-treatment, while in the soil B the increase of priming effect was 4.1-times in the MAI-treatment and 3.7-times in the GLU-treatment. Of the 200 mg  kg⁻¹ added to both soils less than 10 mg NH₃-N kg⁻¹ was volatilized within one day, while 22 and 44 mg  kg⁻¹ soil was fixed on the soil matrix in the soil A and the soil B, respectively. Therefore more than 100 mg −N kg⁻¹ was immobilized into the microbial biomass within the first day. Concentration of nitrite increased sharply in all the treatments of soil A at the onset of the incubation followed by a decrease. A similar pattern was observed in the GLU-N and MAI-N treatments of the soil B, but not in the other treatments. A decrease in concentration of was observed in both soils followed by an increase in the MAI-N and GLU-N treatments of the soil B. It was found that application of had a stimulating effect on the decomposition of maize and glucose, and on the priming effect, while assimilatory reduction of resulted in an increase of in the soil A, and nitrification in the soil B.     

Relationships among dust outbreaks, vegetation cover, and surface soil water content on the Loess Plateau of China, 1999–2000

We analyzed relationships among dust outbreaks, Normalized Difference Vegetation Indices (NDVI), and surface soil water content (0 to 2 cm depth) on the Loess Plateau, a significant dust source area of East Asia. World Surface Data for wind speed and current weather, coarse-resolution data for NDVI, and a three-layer soil model for surface soil water content were used. The threshold NDVI for preventing dust outbreaks was about 0.2 when the wind speed ranged from 7 to 8 m s^− 1. This threshold NDVI corresponds to a vegetation cover of 18%. The threshold ratio of surface soil water content to the field capacity (θ_r) was about 0.2. Conditions facilitating dust outbreaks on the Loess Plateau are when NDVI is less than 0.2 with wind speed  7 m s^− 1 and θ_r < 0.2, and when NDVI is greater than 0.2 with wind speed  9 m s^− 1 and θ_r < 0.2.