Effects of seed-placed sulfur fertilizers on canola,wheat, and pea yield; sulfur uptake; and soil sulfate concentrations over time in three prairie soils

The form of sulfur fertilizer can influence its behavior and crop response. A growth chamber study was conducted to evaluate five sulfur fertilizer forms (ammonium sulfate, ammonium thiosulfate, gypsum, potassium sulfate, and elemental sulfur) applied in seed row at 20 kg S ha^?1 alone, and in combination with 20 kg phosphorus pentoxide (P₂O₅) ha^?1, to three contrasting Saskatchewan soils. Wheat, canola, and pea were grown in each soil for 8 weeks and aboveground biomass yields determined. The fate of fertilizer was evaluated by measuring crop sulfur and phosphorus concentration and uptake, and supply rates and concentrations of available sulfate and phosphate in the seed row. Canola was most responsive in biomass yield to the sulfur fertilizers. Sulfate and thiosulfate forms were effective in enhancing soil-available sulfate supplies in the seed row, crop sulfur uptake, and yield compared to the elemental sulfur fertilizer. Combination of sulfur fertilizer with monoammonium phosphate may provide some enhancement of phosphate availability, but effects were often minor.     

Effectiveness of Gypsum and Pyrite for Sulfur Fertilization on Yield and Quality of Indian Mustard under Rainfed Conditions of North India

Field trials were conducted for two consecutive years during the rabi season in sandy loam soils of Jammu (north India) under rainfed conditions to study the effect of gypsum and pyrite for sulfur (S) nutrition on the oil content, S uptake, and yield of Indian mustard. Application of S through gypsum or pyrite either in natural or ground form increased S content, uptake, oil content, and yield of mustard significantly over the control. Among the different treatments, S application at 50 kg S ha^?1 of ground gypsum (GM₂S₂) recorded the significantly highest mean seed yield of 20.30 and 18.01 q ha^?1, which lead to an increase of 30 and 29.8% in the first and second year, respectively, over the control. For the duration of the experiment, it was observed that 25 kg S ha^?1 was the optimum dose, preferably of ground gypsum, for mustard crops under rainfed conditions.     

Impact of Various Ratios of Nitrogen and Sulfur on Maize and Soil pH in Semiarid Region

Nitrogen and sulfur play an important role in maize production. The aim of this study was to evaluate the effect of nitrogen (N) and sulfur (S) levels applied in various ratios on maize hybrid Babar yield at Peshawar in 2011 and 2013. Four N levels (120, 160, 200 and 240 kg N ha^?1) and four S levels (20, 25, 30 and 35 kg S ha^?1) were applied in three splits: a, at sowing; b, V8 stage; c, VT stage in ratios of 10:50:40, 20:50:30 and 30:50:20. Grains ear^?1, thousand grain weight, grain yield ha^?1 and soil pH were significantly affected by years (Y), N, S and their ratios, while no effect of N, S and their ratios was noted on ears plant^?1. Maximum grains ear^?1 (390), thousand grain weight (230.1 g) and grain yield (4119 kg ha^?1) were recorded in 2013. N increased grains ear^?1 (438), thousand grain weight (252 g) and grain yield (5001 kg ha^?1) up to 200 kg N ha^?1. Each increment of S increased grains ear^?1 and other parameters up to 35 kg S ha^?1, producing maximum grains ear^?1 (430), thousand grain weight (245 g) and grain yield (4752 kg ha^?1), while soil pH decreased from 8.06 to 7.95 with the application of 35 kg S ha^?1. In the case of N and S ratios, more grains ear^?1 (432), heavier thousand grains (246.7 g) and higher grain yield (4806 kg ha^?1) were observed at 30:50:20 where 30% of N and S were applied at sowing, 50% at V8 and 20% at VT stage. It is concluded that 200 kg N ha^?1 and 35 kg S ha^?1 applied in the ratio of 30% at sowing, 50% at V8 and 20% at VT stage is recommended for obtaining a higher yield of maize hybrid Babar.     

Response of Sunflower to Sources and Levels of Sulfur under Rainfed Semi‐arid Tropical Conditions

Sulfur (S) is one of the severely limited nutrients in rainfed semi‐arid tropical Alfisols. Its application plays an important role in improving the yield and quality of oilseed crops. To identify the optimum level of sulfur for greater yield and oil content in the sunflower crop (MSFH‐8) through suitable sources, a field experiment involving varying levels of S through two sources (gypsum and elemental S) in combination with standard levels of nitrogen (N) and phosphorus (P) was conducted on a sandy loam soil (Typic Haplustalf) at Hayathnagar Research Farm of Central Research Institute for Dryland Agriculture, Hyderabad, situated at an altitude of 515 m above mean sea level and on 78° 36′ E longitude and 17° 18′ N latitude. The response to S application in sunflower crop in terms of growth parameters, yield components, nutrient uptake, and seed oil content was conspicuous. The application of graded levels of sulfur at rates of 20, 40, and 60 kg ha^?1 applied through elemental S significantly increased the seed yield of the sunflower crop over the control by 5.4, 10.7, and 18.1% respectively, whereas the corresponding increases in case of gypsum (CaSO₄·2H₂O) were 25.1, 28.8, and 33.9% respectively. The greatest seed yield of sunflower (1175 kg ha^?1) and percentage oil content (39.7%) was obtained with 60 kg S ha^?1 through gypsum under rainfed conditions. Our study clearly indicated that the application of S at relatively high levels significantly increased the uptake of N, P, and S. The percentage oil content in seed recorded a positive and highly significant relationship with the uptake of N (r = 0.958**), P (r = 0.967**), and S (r = 0.951**), signifying the importance of balanced nutrition in influencing the oil content of seed in sunflower. The application of S through gypsum at rate of 60 kg S ha^?1 along with 40 kg N and 30 kg P₂O₅ ha^?1 was most superior in enhancing the seed yield and percentage oil content in seed.     

Impact of sulfur fertilization on distribution of sulfur fractions and use efficiency in blackgram in subtropical acidic soil of Assam,India

A field experiment was conducted to assess the effect of sulfur (S) fertilization on distribution of S in soil and use efficiency on blackgram in subtropical Inceptisol of acidic soil of Assam, India. Five levels of S were applied (0, 10, 20, 30 and 40 kg S ha^?1) along with recommended dose of nitrogen, phosphorus and potassium. Available S content gradually decreased with the advancement of crop growth stages and lowest value was observed at 60 DAS. Different S fractions were found to increase with increasing levels of S application and 40 kg S ha^?1 resulted the highest content for all S fractions. The grain and stover yield of blackgram increased significantly up to 20 kg S ha^?1 which was 95.69% higher over control. Agronomic efficiency, apparent S recovery and recovery efficiency of S were higher at 10 kg S ha^?1and found decreased with increase in level of S.     

Influence of Sulfur Fertility on Wheat Yield Performance on Alluvial and Upland Soils

Abstract: In recent years, sulfur (S) deficiencies in winter wheat (Triticum aestivum L.) have become more common, particularly on coarse‐textured soils. In Study I, field experiments were conducted in 2001/2002 through 2003/2004 on Mississippi River alluvial soils (Experiment I) and an upland, loessial silt loam (Experiment II) to evaluate the influence of spring S rates of 0, 5.6, 11.2, and 22.4 kg ha^?1 and a fall rate of 22.4 kg sulfate (SO₄)‐S ha^?1 on grain yield of three varieties. In Study II, field experiments were conducted in 2001/2002 and 2004/2005 on alluvial soils to evaluate the influence of spring S rates of 0, 5.6, 11.2, and 22.4 kg SO₄‐S ha^?1 in fields where S‐deficiency symptoms were present. Grain yield response to applied S occurred only on alluvial, coarse‐textured, very fine sandy loam soils (Study II) that had soil SO₄‐S levels less than the critical level of 8 mg kg^?1 and organic‐matter contents less than 1 g kg^?1 in the 0‐ to 15‐, 15‐ to 30‐, and 30‐ to 45‐cm depths. Soil pH increased with soil depth. Optimum S rate was 11.2 kg SO₄‐S ha^?1 in 2001/2002 and 5.6 kg SO₄‐S ha^?1 in 2004/2005. On the upland, loessial silt loam soil, soil SO₄‐S levels accumulated with depth, whereas organic‐matter content and pH decreased. In the loessial soils, average soil SO₄‐S levels in the 15‐ to 30‐ and 30‐ to 45‐cm soil depths were 370% greater than SO₄‐S in the surface horizon (0 to 15 cm).     

EFFECT OF NITROGEN AND SULFUR FERTILIZATION ON YIELD COMPONENTS,SEED AND OIL YIELDS OF CANOLA

Field experiments were conducted to evaluate the effects of nitrogen (N) and sulfur (S) levels and their methods of application on canola. Branches plant^?1, pods plant^?1 and biological yield significantly increased with increase in nitrogen level and no significant increase in seed pod^?1 and seed and oil yields occurred beyond 120 kg N ha^?1. However, thousand seed weight consistently decreased with increasing level of nitrogen. Pods plant^?1 and biological yield continually increased with increase in sulfur level. Alternatively, significant increase in branches plant^?1, seed pod^?1, seed weight, seed and oil yields was noted with increase in sulfur level up to 40 kg ha^?1. Applications of sulfur and nitrogen in split significantly decreased seed yield as compared to sole applications. It is concluded that sulfur and nitrogen application as sole at the rate of 40 and 120 kg ha^?1, respectively performed better than the rest of their levels and method of application.     

Transformation of S Fractions under Field Conditions with Groundnut (Arachis hypogaea L.) in an Alfisol of Karnataka

A field experiment involving four levels of sulfur (S; 0, 15, 30, and 45 kg ha^?1) and three sources [elemental S, gypsum, and ammonium sulfate] significantly increased contents of all the S fractions compared to no S application and which decreased with crop growth. Application of ammonium sulfate recorded greater values of S fractions compared to gypsum and elemental S. Lower values of S fractions were recorded in elemental S treatments. Significant decreases in water-soluble and available soil S between flowering and harvest contributed to the pool of plant-available S. Significant increases in residual S fractions were observed due to levels of applied S even after harvest. Correlations between the S fractions indicated significant positive relations. Greatest pod and haulm yields, oil content, and oil yield of the groundnut were recorded in the T₁₀ treatment followed by gypsum and elemental S treatments.     

Effect of Direct and Residual Effects of Different Sulfur Fertilizers on Groundnut and Wheat Cropping System on Typic Haplaquent Soils

Abstract

A field experiment was conducted on sulfur deficient soil during kharif dry seasons of 1993, 1994, and 1995 to study the comparative efficacy of selected sources of sulfur to groundnut (Arachis hypogaea L.). The results of the experiment were tested on farm trial in cultivator's field during the kharif season of 1999. The yield of groundnut crop in experimental field increased significantly with increasing levels of sulfur up to 40 kg S ha^?1. Among the various sources tested, gypsum was the best with respect to yield, followed by single super phosphate and ammonium sulfate. In on‐farm trial the application of gypsum at 20 kg S ha^?1 increased the yield parameters and yield over un‐treated control. The residual effect of different sulfur sources on wheat (Triticum aestivum) grain yield, the effect of treatments was non significant but the levels of sulfur and the interaction between source and levels were significant.     

Improving Yield and Quality of Palmarosa [Cymbopogon Martinii (Roxb.) Wats. Var. Motia Burk.] with Sulfur Fertilization

A field experiment was conducted during 2006–2007 at Hyderabad, India on sulfur-deficient alfic ustochrept soil on aromatic crop palmarosa [Cymbopogon martinii (Roxb.) Wats. var. motia Burk.] with five rates of sulfur (S) application (0, 10, 20, 30, and 40 kg ha^?1). Four harvests were performed between August 2006 and August 2007 at 90-day intervals. A fifth harvest was taken in November 2007 to study the residual effect of S. Five constituents accounting for 87.4–98.7% of the essential oil were identified by gas chromatography-flame ionization detector (GC-FID) and gas chromatography mass spectroscopy (GC-MS) analyses. The results revealed that 40 kg S ha^?1 produced the highest total (total of four harvests) biomass yield (71.5 t ha^?1), and total essential oil yield (382.3 kg ha^?1). The highest contents of essential oil components cis-β-ocimene (2.5%), linalool (2.9%), geraniol (84.9%), geranyl acetate (9.8%), and geranyl hexanoate (2.6%) were observed in 40 kg S ha^?1 treatment. Sulfur exhibited no residual effect, therefore application is recommended to each harvest.     

Response of Sunflower to Sulfur Rate and Time of Application under Two Tillage Systems in a Silty Clay Soil of Dera Ismail Khan Pakistan

ABSTRACT

Sulfur (S) availability is a characteristic of conservation tillage. We studied the effects of S on sunflower yield and fatty acid profile under conventional and conservation tillage in silty clay soil of D.I. Khan, Pakistan. Conventional tillage consisted of disk plowing followed by tiller and rotavator, while conservation tillage comprises tiller and disc harrowing. Treatments comprise six sulfur levels (S₁ = 0, S₂ = 20 kg S ha^?1 at sowing, S₃ = 10 kg S ha^?1 at sowing +10 kg S ha^?1 25 days after sowing (DAS), S₄ = 40 kg S ha^?1 at sowing, S₅ = 20 kg S ha^?1 at sowing + 20 kg S ha^?1 25 DAS, and S₆ = 20 kg S ha^?1 at sowing + 10 kg S ha^?1 25 DAS + 10 kg S ha^?1 50 DAS) replicated thrice. After 2 years of experimentation, soil samples were collected (from 0 to 0.30 m depth) for organic matter (OM), total soil N (TSN), and available sulfur analysis. Conservation tillage significantly increased OM, TSN, and S contents in 0–0.30 m soil compared to conventional tillage. Likewise, sunflower plants showed higher achene yield, better fatty acid profile, and net economic returns in conservation tillage than in conventional tillage. Application of 20 kg S ha^?1 at sowing and 10 kg S ha^?1 at each 25 and 50 DAS recorded significantly higher achene yield, higher oil, and linoleic acid contents; however, oleic and oleic/linoleic ratio did not increase with higher S rates. Conservation tillage was effective in increasing OM, TSN, and available S besides increasing yield and economics in silty clay soil.     

Inoculation of rapeseed under different rates of inorganic nitrogen and sulfur fertilizer: impact on water relations,cell membrane stability,chlorophyll content and yield

It is important to develop integrated fertilization strategies for various crops that enhance the competitive ability of the crop, maximize crop production and reduce the risk of nonpoint source pollution from fertilizers. In order to study the effects of mineral nitrogen fertilization and biofertilizer inoculation on yield and some physiological traits of rapeseed (Brassica napus L.) under different levels of sulfur fertilizer, field experiments in factorial scheme based on randomized complete block design were conducted with three replications in 2012 and 2013. Experimental factors were: (1) four levels of chemical nitrogen fertilizer (0, 100, 150 and 200 kg N ha^?1), (2) two levels of biofertilizer (with and without inoculation) consisting Azotobacter sp. and Azospirillum sp. and (3) two levels of sulfur application (0 and 50 kg S ha^?1). Rapeseed yield, oil content of grains and studied physiological traits had a strong association with the N fertilization, biofertilizer inoculation and sulfur (S) application. Higher rates of N fertilization, biofertilizer inoculation and S application increased the grain yield of rapeseed. In the case of physiological traits, the highest value of relative water content (RWC) was recorded in 100 kg N ha^?1 that was statistically in par with 150 kg N ha^?1 application, while usage of 150 kg N ha^?1 showed the maximum cell membrane stability (CMS). Inoculation with biofertilizer and S fertilization resulted in higher RWC and CMS in rapeseed plants. The chlorophyll content showed its maximum values in the highest level of N fertilization, biofertilizer inoculation and S application. The usage of 200 kg N ha^?1 significantly decreased the oil content of rapeseed grains, but the highest grain oil content was obtained from the application of 150 kg N ha^?1, Azotobacter sp. and Azospirillum sp. inoculation and S fertilization. It seems that moderate N rate (about 150 kg N ha^?1) and S application (about 50 kg S ha^?1) can prove to be beneficial in improving growth, development and total yield of inoculated rapeseed plants.     

EFFECTS OF DIFFERENT HARVEST TIME AND SULFUR FERTILIZATION ON AMINO ACID COMPOSITION OF LENTIL

The effects of sulfur (S) fertilization and harvest time on amino acid composition of seeds of field-grown lentil (Lens culinaris Medik.) at two different sites were studied. The aim of this study was to determine amino acid content of seed protein and to increase low levels of sulfur amino acids and trytophan in lentil seeds, which are major components for grain quality, with sulfur fertilization and farming practices in lentil. For this purpose, lentil was sown at two locations on 18 and 19 October 2007, 0 kg S ha^?1, 25 kg S ha^?1, and 50 kg S ha^?1 rates were used in the study. The plants were harvested at three different periods: early, optimal, and late period. According to the results, amino acid contents of protein were increased by sulfur fertilization and by the late harvest period.     

INFLUENCE OF NITROGEN AND SULFUR ON YIELD AND SEED QUALITY OF THREE CANOLA CULTIVARS

In order to investigate the effect of nitrogen (N) and sulfur (S) fertilizers on yield and seed quality of three canola cultivars, a factorial based on randomized complete block experiment was conducted during 2005–2006 in Iran. Treatments included four nitrogen rates (0, 75, 150, and 225 kg N ha^?1 source of urea), four sulfur rates (0, 100, 200, and 300 kg S ha^?1), and three cultivars (‘Pf’, ‘Option-500’, and ‘Hyola-401’). Results indicated cultivar had a significant effect on all studied traits. ‘Option-500’ and ‘Hyola-401’ cultivars had the highest seed yield, protein content, and N:S ratio in seed. The levels of 150 and 220 kg N ha^?1 resulted in the maximum protein content. Increasing N levels resulted in N content and decreased the oil content. The interaction effect between S and N levels showed the highest N content in seed was obtained with 300 kg S ha^?1 and 225 kg N ha^?1.     

Response of maize for grain to potassium and magnesium fertilizers in soils with high lime contents

An antagonistic reaction between calcium (Ca) and magnesium (Mg) and potassium (K) may lead to low absorption of K and Mg by plants from soils with high Ca contents even when levels of K and Mg should be adequate. Two separate field studies were carried out in 2009 and 2010 to determine the effects of potassium (0, 40, 80, 120 kg K₂O ha^?1; as potassium sulfate) and magnesium (0, 20, 40, 60 kg magnesium oxide (MgO)ha^?1; as magnesium sulfate) applied to a soil with high lime content either separately or in combinations, on the grain yield and yield components of maize for grain in semi-arid Central Anatolia in Turkey. One dose of the K, Mg-fertilizers was applied during sowing in both years. According to the results, increasing the dosage of K increased yield components more than increases in Mg dosages. Combinations of K and Mg tended to maximize the yield components. Moreover, the greatest plant heights, first ear lengths, grain weights per ear and protein ratios were obtained for the K₈₀Mg₄₀ dose.     

IMPACT OF SULFUR FERTILIZATION ON DIFFERENT FORMS AND BALANCE OF SOIL SULFUR AND THE NUTRITION OF WHEAT IN WHEAT-SOYBEAN CROPPING SEQUENCE IN TARAI SOIL

A field experiment was conducted at farmer's field in Mollisols of Tarai soils in Uttarakhand (India), to assess the direct and residual effect of sulfur fertilization in wheat-soybean cropping sequence. Four levels of sulfur (S; 0, 15, 30, and 45 kg ha^?1) were applied to main crop (wheat) along with recommended dose of nitrogen (N), phosphorous (P), and potassium (K). The direct and residual effect of sulfur at highest level showed 27 and 6 percent increase in grain yield of wheat and soybean over control, respectively. The increase in grain yield of wheat was significant at each sulfur level. The direct as well as residual effect of sulfur showed significant increase in sulfur concentration and its uptake by grain and straw except increase in sulfur concentration and uptake by soybean straw. In wheat-soybean cropping sequence, the agronomic efficiency and apparent sulfur recovery decreased with increase in levels of sulfur, but the percent response increased with increasing sulfur application. Different forms of sulfur such as total sulfur, organic sulfur, calcium chloride extractable sulfur, potassium dihydrogen phosphate extractable sulfur, and non-sulfate sulfur in post-harvest soil increased according to sulfur level applied but it decreased under control and also after residual crops. The buildup of sulfur in surface soils was greater than in the deeper soils. Application of sulfur showed the positive sulfur balance and it increased with increase in sulfur level, while it was negative under control. A major portion (46–62%) of applied sulfur contributed to increase in sulfur content of root zone soil followed by unaccounted component (25–40%) and small portion (11–18%) was absorbed by wheat plant as uptake.     

ELEMENTAL SULFUR IMPREGNATED WITH IRON AS A FERTILIZER SOURCE FOR KENTUCKY BLUEGRASS

Elemental sulfur (S) impregnated with Fe (S-Fe) fertilizer was evaluated on Kentucky bluegrass (KBG; Poa pratensis L.) in three glasshouse studies: (1) with/without Fe as S-Fe, ferrous sulfate (FS), or iron-ethylenediamine-N,N’-bis(2-hydroxyphenylacetic acid) (Fe-EDDHA) to soil (49 kg Fe ha^?1), or foliar Fe-EDDHA (5 kg Fe ha^?1) on two cultivars; (2) 0, 49, 197, or 592 kg Fe ha^?1 of S-Fe deep mixed or surface mixed on two soils; (3) 0 or 49 kg Fe ha^?1 applied as S-Fe on four cultivars. A field study comparing 0 and 49 kg Fe ha^?1 applied as S-Fe to KBG was also conducted. Soil applied S-Fe was assimilated in shoots as efficiently as Fe-EDDHA applied to soil or foliage. Shoot Fe increased significantly with increasing S-Fe in a curvilinear response when deep mixed with soil and in a linear response when surface applied. However, no Fe source tested impacted yield or verdure in these studies.     

Response of sunflower to potassium and magnesium fertilizers in calcerous soils in central Anatolia of Turkey

This research was carried out to determine the effects of potassium [0, 40, 80, 120 kg potassium oxide (K₂O) ha^?1] and magnesium (0, 20, 40, 60 kg magnesium oxide (MgO) ha^?1) applied into soil separately and together on the grain yield and yield components of sunflower for oil grown in two farmer fields in the semi-arid Central Anatolia in 2009 and 2010. The experiments were set as factorial experiment design in randomized blocks and 4 replicates. Potassium and Mg-fertilizers were used in the single time into base in the sowing. According to the results, K application in the increasing doses increased yield components more than that of Mg. Together giving of the K and Mg in certain combinations took the yield components to maximum levels. The highest grain yields were obtained by the K₄₀Mg₄₀ in the first year (7313 kg ha^?1) and by the Mg₆₀ in the second year (6510 kg ha^?1).     

Effect of Cattle Manure and Sulfur on Yield and Oil Composition of Pumpkin (Cucurbita pepo var. Styriaca) Inoculated with Thiobacillus thiooxidans in Calcareous Soil

The effect of cattle manure and sulfur fertilizer on seed yield and oil composition of pumpkin (Cucurbita pepo var. Styriaca) under inoculated with Thiobacillus thiooxidans was investigated in a factorial study based on a randomized complete block design. Experimental factors consisted of cattle manure (M) (M₀: 0, M₁: 10; and M₂: 20 t ha^?1), sulfur (S) (S₀: 0, S₁: 250; and S₂: 500 kg ha^?1) and T. thiooxidans (B): inoculated (B₁) and non-inoculated (B₀). Results demonstrated that the application of T. thiooxidans, cattle manure, and S fertilizer decreased the soil pH. The largest number of seed per fruit (367), highest fruit yield (70.57 t ha^?1), seed iron (Fe) content (16.26 mg 100 g^?1), and seed yield (111 kg ha^?1) was obtained when 20 t ha^?1 manure was applied in combination with 500 kg ha^?1 S inoculated with T. thiooxidans. In this condition, the content of S, Fe, phosphorus (P), and nitrogen (N) in plant shoots was increased by 44.8%, 22.58%, 33.89%, and 10.38%, respectively, compared to the control. Moreover, the highest content of seed protein was observed in 10 t ha^?1 manure and 500 kg ha^?1 S fertilizer inoculated with T. thiooxidans. When 250 kg ha^?1 S fertilizer was applied, 20 t ha^?1 manure decreased seed P content sharply. At the rate of 500 kg ha^?1 S fertilizer, the highest content of seed P was obtained from 20 t ha^?1 manure. Totally, 20 t ha^?1 cattle manure, along with 500 kg ha^?1 S fertilizer as well as T. thiooxidans inoculation, improved oil and seed yield of medicinal pumpkin.     

NITRATE ACCUMULATION IN SPINACH AS INFLUENCED BY SULFUR AND PHOSPHORUS APPLICATION UNDER INCREASING NITROGEN LEVELS

Nitrate (NO₃) accumulation by spinach was studied under increasing nitrogen (N) levels (60, 120 and 240 kg N ha^?1) along with sulfur (45 kg S ha^?1) and phosphorus (P; 90 kg P₂O₅ ha^?1) application. Plants were harvested at 50 and 65 days after sowing. Plant samples were analyzed for NO₃-N and total N, P, S, potassium (K), calcium (Ca), and magnesium (Mg). Radio assay of 35S was done to estimate percent sulfur derived from fertilizer and percent fertilizer sulfur utilization. Spinach maintained a very high level of NO₃-N in its tissue throughout the growing period. NO₃-N was increased with increasing nitrogen level and was reduced with phosphorus and sulfur application and also with advancement in growth. Total N, P, S, K, Ca and Mg uptake were increased with increasing nitrogen levels as well as with application of sulfur and phosphorus. Sulfur application caused increase in percent sulfur derived from fertilizer and percent utilization of fertilizer sulfur.