Comparative response of corn and soybean to seed‐placed phosphorus over a range of soil test phosphorus

Abstract

The responses of corn and soybean to seed‐placed fertilizer were compared over NaHCO₃‐extractable soil phosphorus (P) levels ranging from 3 to 35 ppm in a two‐year experiment. Early season corn and soybean shoot‐P concentrations were increased with increasing soil test P and were increased with seed‐placed P regardless of soil test P, although the increases were greater for corn than soybean. Corn grain yield increased with increasing soil test P to a plateau level and increased with seed‐placed P regardless of soil test P. A side‐band (5 cm × 5 cm) application of 39 kg P ha^‐1 at a low soil test P increased yield more (P<0.15) than application of 7 kg P ha^‐1 with the seed. A side‐band application of 9 kg P ha^‐1 at a medium soil P test did not increase yield. Soybean yield was increased with increasing soil test P one year out of two, but did not respond to seed‐placed P in either year. The yield response of corn was attributed to the increased P concentration prior to the 6‐leaf stage.     

Measurement of available soil phosphorus under conventional and no‐till management

Abstract

The objective of the investigation was to compare the predominant forms of P in fields of an acid Matapeake soil under no‐till and conventional‐till management. The fields, which differed in extent of contact between soil and fertilizer P, also differ in forms of P. Fertilizer P remained in the uppermost layer of no‐till fields and was, therefore, not in as close proximity to the plant (corn) roots as P was in conventional‐till fields. Nevertheless, fertilizer P in the no‐till fields underwent only limited conversion to forms less available to plants and thus, in part, may have accounted for similar plant uptake rates of P in no‐till and conventional‐till fields.

One method used to analyze P in the Matapeake soil was the double‐acid‐extraction method. This method is used by several state soil‐testing laboratories of the eastern and southeastern United States, where acid soils like Matapeake are common. The double‐acid‐extraction method underestimated the soil P available for plant uptake.     

Zinc‐efficient wild grasses enhance release of phytosiderophores under zinc deficiency

The effect of the zinc (Zn) nutritional status on the rate of phyto‐siderophore release was studied in three wild grass species (Hordeum murinum, Agropyron orientale, and Secale cereale) grown in nutrient solution under co‐trolled environmental conditions. These wild grasses are highly “Zn‐efficient”; and grow well on severely Zn‐deficient calcareous soils in Turkey (DTPA‐extractable Zn was 0.12 mg/kg soil and CaCO₃ was 37%). In all wild grasses studied, Zn deficiency reduced shoot growth but had no effect on root growth. Low amounts of phytosiderophores were released from roots of all wild grasses adequately supplied with Zn. In plants grown without Zn, release of phytosiderophores progressively increased with the onset of visual Zn deficiency symptoms, such as inhibition of shoot elongation and appearance of chlorotic and necrotic patches on leaves. Compared to Zn‐sufficient plants, phytosiderophore release increased 18–20‐fold in deficient plants. HPLC analysis of root exudates showed that the dominating phytosiderophore in Zn‐deficient Agropyron and Hordeum was 3‐epi‐hydro‐xymugineic acid (epi‐HMA) and was 3‐hydroxy‐mugineic acid (HMA) in Secale. Besides HMA, epi‐HMA and mugineic acid (MA) were also detected in exudates of Zn‐deficient Secale. The results indicate the importance of phytosiderophores in adaptation of wild grasses to Zn‐deficient calcareous soils. Phytosiderophores might enhance mobilization of Zn from sparingly soluble Zn pools and from adsorption sites, both in the rhizosphere and within the plants.     

Variation of early growth and nutrient content of no‐till corn and soybean in relation to soil phosphorus and potassium supplies

Abstract

Knowledge of relationships between variation in early plant growth and soil nutrient supply is needed for effective site‐specific management of no‐till fields. This study assessed relationships between soil test phosphorus (STP) and potassium (STK) with early plant growth and P or K content of young corn (Zea mays L.) and soybean [Glycine max (L.) Merr.] plants in eight no‐till fields. Composite soil (0–15 cm depth) and plant (V5‐V6 growth stages) samples were collected from 400‐m² areas at the center of 0.14‐ha cells of a 16‐cell square grid and from 2‐m² areas spaced 3 m along each of two 150‐m intersecting transects. Correlation, regression, multivariate factor analyses were used to study the relationships between the variables. Variability was higher for samples collected from the transects. Plant dry weight (DW), P uptake (PU), and K uptake (KU) usually were correlated with STP and STK but the correlations varied markedly among fields. Relationships between soil and plant variables could not always be explained by known nutrient sufficiency levels for grain production. Plant P concentration (PC) was not always correlated with STP and sometimes it increased linearly with STP, but other times increased curvilinearly until a maximum was reached. Plant K concentration (KC) usually was correlated with STK, however, and increased linearly with increasing STK even in fields with above‐optimum STK. The results suggest greater susceptibility of early growth to STP than to STK and greater plant capacity to accumulate K compared with P over a wide range of soil nutrient supplies. Variation in STK likely is a major direct cause of variation in KC over a wide range of conditions but variation in STP is not likely a major direct cause of variation in PC when high STP predominates.     

Differential response of groundnut genotypes to iron‐deficiency stress

Differential response of groundnut genotypes to iron‐deficiency stress was studied in soils containing high calcium carbonate. Genotypes differed significantly for some traits that appeared to be important in determining adaptation to low iron. The genotypes TCGS 273, TCGS 2, TCGS 37, and Kadiri 3 had higher total chlorophyll, total dry matter, and active iron (Fe²⁺) contents under iron‐deficiency stress conditions. Total chlorophyll followed by active iron were found to be sensitive parameters to Fe deficiency. Based on the visual deficiency symptoms (chlorosis score), the genotypes were classified into three groups. Efficient (no genotype was found efficient), moderately efficient (TCGS 273, TCGS 2, TCGS 3, and Kadiri 3), and inefficient (TCGS 1, TCGS 7, TCGS 11, TCGS 26, TCGS 28, TCGS 29, TCGS 30, TCGS 1518, TPT 1, TPT 2, ICGS 11, ICGS 44, Girnar, JL 24, ICGS(E) 21, and TMV 2).     

Pear seedling response to phosphorus in a phosphorus‐fixing soil

Abstract

The effect of P on growth of ‘Bartlett’ pear (Pyrus communis L.) seedlings was evaluated on the P‐fixing, Parkdale soil (Vitrandepts) from Oregon, USA. The P treatment levels were 0.03, 0.04, 0.06, 0.09, 0.15, 0.25, and 0.40 mg P/L of soil solution, based on a P‐sorption isotherm. At age 145 days, the dry weight response to P was significant. Seedlings required 0.25 mg P/L for maximum growth. This corresponds to 723 mg P/kg soil based upon the P‐sorption isotherm. Standard P soil tests did not accurately predict seedling response to P. About one‐half of the soil P was in the chemisorbed fraction.     

Effect of various phosphorus availabilities on radiation‐use efficiency in sunflower biomass until anthesis

Sunflower (Helianthus annuus L.) cv. SELECT was grown in 1994 on a 25 year long‐term phosphorus (P) field experiment. Phosphorus rates applied annually are: 0 (PO), 17.5 (P1), 35 (P2), and 70 (P4) kg P/ha. The experiment confirms the seasonal trend of Radiation‐Use Efficiency (RUE) over the growing period from B4/B5 to F1 stage. RUE values ranges were: P0 (0.8–2.5), P1 (1.0–2.6), P2 (1.2–3.0), and P4 (1.2–3.7) with accumulated IPAR ranging from 25 to roughly 250 MJ/m². Sunflower RUE was insensitive to or only weakly sensitive to P nutritional conditions ranging from poor (P0) to moderate (P1). By contrast, RUE of sunflower grown in high (P2) to very high (P4) available P conditions were much higher at any time of the growing period. In soils well endowed in P, it may be worth taking into account this RUE sensitivity for modelling dry matter accumulation in sunflower.     

Phosphorus‐deficiency effects on response of symbiotic N2 fixation and carbohydrate status in soybean to atmospheric CO2 enrichment

The impact of phosphorus (P) deficiency on response of symbiotic N₂ fixation and carbohydrate accumulation in soybean (Glycine max [L.] Merr.) to atmospheric CO₂ enrichment was examined. Plants inoculated with Bradyrhizobium japonicum MN 110 were grown in growth chambers with controlled atmospheres of 400 and 800 μL CO₂ L^‐1 and supplied either 1.0 mM‐P (P‐sufficient) or 0.05 mM‐P (P‐deficient) nitrogen (N)‐free nutrient solution. When plants were supplied with sufficient P, CO₂ enrichment significantly increased whole plant dry mass (83%), nodule mass (67%), total nitrogenase activity (58%), and N (35%) and P (47%) accumulation at 35 days after transplanting (DAT). Under sufficient P supply, CO₂ enrichment significantly increased starch concentrations in nodules compared to the normal atmospheric CO₂ treatment. Under normal CO₂ levels (400 μL L^‐1) nonstructural carbohydrate concentration (starch plus soluble sugar) was significantly higher in leaves of P‐deficient plants than in leaves of P‐sufficient plants in which nonstructural carbohydrate concentration exhibited a strong diurnal pattern. Under deficient P supply whole plant dry mass, symbiotic N₂‐fixation parameters, and N and P accumulation were not enhanced by atmospheric CO₂ enrichment. Phosphorus deficiency decreased nonstructural carbohydrate accumulation in nodules at the end of a 10‐day period in which functional activity was developing by 86% relative to P‐sufficient controls. While P deficiency elicited significant increases in the nonstructural carbohydrate concentration in leaves, it caused significant decreases in the nonstructural carbohydrate concentration in nodules over the diurnal cycle from 30 to 31 DAT. Collectively, these results indicate that the lack of a symbiotic N₂‐fixation response to atmospheric CO₂ enrichment by P‐deficient plants may be related to the decreased carbohydrate status of nodules.     

Response of soybean to foliar‐applied boron and magnesium and soil‐applied boron 1

Annual plants may partition carbon (C) preferentially to reproductive structures slowing root elongation and subsequent nutrient uptake. Although foliar applications of nitrogen (N), phosphorus (P), potassium (K), and sulfur (S) supplement uptake by roots, soybean [Glycine max (L.) Merr.] yield increases have not been found in most studies. Experiments were designed to determine if foliar applications of boron (B), magnesium (Mg), or B+Mg would increase soybean yield and if soybean would respond to B applied to the soil several weeks prior to planting. Foliar B or Mg applied separately four times during reproductive growth did not affect soybean yield. However, four foliar applications of B+Mg increased soybean yield 12% at Mt. Vernon and 4% at Columbia over a three‐year period. Two foliar applications of B+Mg during the late reproductive stages increased soybean yield 8% over a two‐year period. The yield increase from foliar B+Mg treatment resulted from an increased number of pods on the main stem (18%) and branches (44%). A 2.8 kg/ha B application to soil eight weeks prior to planting increased soybean yield 11% during the first year and 13% the second year but had no effect on soybean yield by the third year after application. When results from the first two years were combined, 2.8 kg/ha B applied to soil increased the number of pods per branch by 17% and the number of branch pods per plant by 39%. Foliar applications of B+Mg increased soybean yield in four of six site‐years in the three‐year experiments at two locations.     

A comparison of methods for estimating phosphorus uptake kinetics under steady‐state conditions 1

Michaelis‐Menten kinetic parameters (I_max and K_M) are useful for describing nutrient uptake by plants. This paper compares two methods for estimating the kinetics of P uptake. Both methods employed a steady‐state hydroponic system to measure P uptake by wheat (Triticum aestivum L.) seedlings. In one method, uptake was measured from two P concentrations in nutrient solution, with I_max and K_M estimated by direct linear plot (DLP). In an alternate, multiple concentration (MC) method, uptake was measured from five P concentrations, and kinetic parameters were estimated by either nonlinear regression or the Hanes plot. The I_max and K_M, estimates obtained by the DLP method were compared to those obtained by the MC method. The MC method offered practical advantages. Unlike the DLP, it allowed estimation of the external P concentration at which net influx = 0 (C_min), and did not require a priori estimates of K_M and C_min. The MC method provided more precise median parameter estimates as indicated by smaller nonparametric confidence intervals. Using the median C_min value of 1.9 μM, the best estimates of I_max and K_M (and 96% confidence intervals) derived by nonlinear regression were 2.2 (1.6 to 2.8) nmol P g^‐1s^‐1, and 11 (10.6 to 12.9) μM, respectively.     

Response to iron‐deficiency stress of pear and quince genotypes 1

Roots of iron (Fe)‐efficient dicots react to Fe‐deficiency stress by strongly enhancing the ferric (Fe³⁺)‐reductase system and by lowering the rhizo‐sphere pH. In this study, we tested whether such adaptation mechanisms characterize pear and quince genotypes known to have differential tolerance to calcareous and alkaline soils. Two trials were performed using micropagated plants of three quince rootstocks (BA29, CTS212, and MC), three Pyrus communis rootstocks (OHxF51 and two selections obtained at the Bologna University: A28 and B21) and of two pear cultivars (Abbé Fétel and Bartlett, own‐rooted). In the first trial, plants were grown in a nutrient solution with [Fe(+)] and without [Fe(‐)] Fe for 50 days. Their root Fe‐reducing capacity was determined colorimetrically using ferrozine and FeEDTA, and Fe uptake of Fe(+) plants was estimated. In the second trial, the rhizosphere pH of plants grown in an alkaline soil was measured by a micro‐electrode. With the only exception of pears OHxF51 and A28, whose Fe‐reduction rates were similar in Fe(+) and Fe(‐) plants, the Fe‐deficiency stress resulted in a significant decrease in Fe reduction. Among the Fe(‐) plants, the two pear cultivars, OHxF51 and A28, had a higher Fe‐reducing capacity than the quince rootstocks and the cv. Abb6 F. When plants were pre‐treated with Fe, reduction rate was highest in the P. communis rootstocks, intermediate in the own‐rooted cultivars, and lowest in the quinces. Root Fe‐reducing capacity of Fe(+) plants proved to be linearly and positively correlated with Fe uptake and root proton release. Rhizosphere pH was highest in quince MC, intermediate in the other two quinces and in the cv. Abbe F., and lowest in the pear rootstocks and in the cv. Bartlett. Our results indicate that roots of pear and quinces do not increase their ability to reduce the Fe under Fe‐deficiency stress. The genotypical differential tolerance to Fe chlorosis likely reflects differences in the standard reductase system and in the capacity of lowering the pH at the soil/root interface. The determination of the root Fe‐reducing capacity is a promising screening technique for selecting pear root‐stocks efficient in taking up Fe.     

Root responses of sterile‐grown onion plants to iron deficiency 1

Onion (Allium sativum) plants grown without iron (Fe) in sterile nutrient solutions readily developed chlorosis symptoms. Iron deficiency in the sterile‐grown plants stimulated the rates of root extracellular reduction of Fe³⁺, copper (Cu²⁺), manganese (Mn⁴⁺), and other artificial electron acceptors. While rapid reduction occurred with the synthetic chelate Fe³⁺HEDTA, no short‐term reduction occurred with the fungal siderophore Fe³⁺ferrioxamine B (FeFOB). In addition to the increased rate of extracellular electron transfer at the root surfaces, the Fe‐deficient plants showed greater rates of Fe uptake and translocation than the onion plants grown with Fe. The rates of uptake and translocation of Fe were sharply higher for the Fe‐deficient plants supplied with FeHEDTA than for similar plants supplied with FeFOB. Inhibition by BPDS of the Fe uptake by the Fe‐deficient onion plants further supported the importance of Fe³⁺ chelate reduction for the uptake of Fe into the roots. Rates of Fe uptake and translocation by Fe‐deficient onion plants supplied with ⁵⁵FeFOB were identical to the rates of uptake of ferrated [14C]‐FOD; a result that gives evidence of the uptake and translocation of the intact ferrated siderophore, presumably by a mechanism not involving prior extracellular Fe³⁺ reduction. Differences in the rates of transport of other micronutrients into the roots of the Fe‐deficient onion plants were evident by the significantly higher Zn and Mn levels in the shoots of the Fe‐deficient onion.     

Effect of sewage sludge application to amelio‐rate iron deficiency of grain sorghum

Abstract

Recent research has indicated that land application of municipal sewage sludge to calcareous soils can be used to ameliorate iron (Fe) deficiency of grain sorghum [Sorghum bicolor (L.) Monech]. A greenhouse study was conducted to determine the response of grain sorghum grown on three different soils to application of sewage sludge. Sludge applied at rates of 0, 7.5, 15.0, and 25.0 g/kg soil did not completely ameliorate grain sorghum Fe deficiency. When FeEDDHA was soil applied, sewage sludge application significantly increased plant growth due to increases in soil phosphorus (P) availability. Application of sewage sludge at rates greater than 7.5 g/kg reduced dry matter production of grain sorghum in the FeEDDHA amended Orelia SC soil, the soil with the lowest total neutralizing potential. The decreases yield was possibly due to toxic levels of soil and plant copper (Cu) and zinc (Zn), and increased soil salinity.     

Comparison of phosphate‐phosphorus and total phosphorus in DTPA extracts for assessing plant‐available phosphorus in soilless potting media

Abstract

Hakea francisiana and H. laurina were grown in soilless media based on pine bark, to which had been added one of the following phosphorus (P) sources: crushed bone, rock phosphate, calcined rock phosphate, sewage sludge, or sludge compost. Available P was assessed through extraction with unbuffered 2 mM DTPA. Similar regression equations between shoot P content and P in 2 mM DTPA extracts of the media at potting were obtained for both total P in the extract (determined by inductively coupled plasma emission spectrometry) and PO₄‐P. The difference between them was small compared with the variation caused by different rates of dissolution of P the various sources during the growing period. Extractants give only an approximate guide to plant P uptake when the medium contains sources that slowly dissolve during the growing period. Nevertheless, the data indicate that, irrespective of P source, the maximum P concentration in a 2 mM DTPA extract (1:1.5 v/v) of the medium that is tolerated by P‐sensitive plants is 3–4 mg/L. This is similar to the concentration found previously for superphosphate as the source of P.     

Colorimetric determination of phosphorus in citrate‐bicarbonate‐dithionite extracts of soils

Abstract

Existing colorimetric methods for determining phosphorus (P) in citrate‐bicarbonate‐dithionite (CBD) extracts from soils and sediments require pretreatments of extracts prior to the determination of the element. A method is proposed for the determination of P in CBD extracts without any chemical treatments. Maintaining the citrate concentration below 2.4 mM, maximum and stable absorbance develops in P‐Mo‐Sb system within one hour and remains stable for 20 hours. An evaluation of potential interference was made by adding varying amounts of P to CBD extracts over a range of extractable iron (Fe) and aluminum (Al) levels. Near 100 percent recovery of the added P suggests the applicability of the method to soils and sediments. Major advantages of the method are speed, simplicity, reliability and stability of the colored complex.     

A preliminary comparison of the ammonium acetate‐edta soil phosphorus extraction method to the bray‐1 and olsen soil phosphorus extraction methods

Abstract

The ammonium acetate (NH₄OAc)‐EDTA soil phosphorus (P) extraction method was compared to either the Bray‐1 soil P extraction method for non‐calcareous soils or the Olsen soil P extraction method for calcareous soils to predict com and wheat plant tissue P concentration and grain yield responses. The NH₄OAc‐EDTA method predicted yield and tissue P concentration responses to P fertilizer applications more accurately than the Olsen method at three of five sites. Both the Bray‐1 and NH₄OAc‐EDTA methods were successful in predicting corn and wheat yield responses to P fertilizer applications in non‐ calcareous soils in many locations. However, a direct comparison of extracted soil P levels showed that the NH₄OAc‐EDTA method extracted soil P at levels which were more closely related to the Bray‐1 method than the Olsen method.     

Short‐term evolution of phosphorus in an ultisol

Abstract

Evolution of residual phosphate was monitored as function of time on an Ultisol without cropping in a field trial at Sembawa Rubber Research Station, South Sumatra, Indonesia. Three treatments were imposed and either triple superphosphate at a rate of 600 kg ha^‐1 containing 21.54% phosphorus (P) or lime at the rate of 4.14 tons ha^‐1 was applied. During the 20 months of incubation, extractable phosphate as evaluated by hydrochloric acid‐ammonium fluoride (Bray‐I) declined in all treatments. The decrease in the P fertilized plot was more pronounced in relation to the control and the limed soil. Only 56% of P added to the soil was initially recovered as extractable P. This percentage was 30–40% in limed soils. No significant pH difference was recorded for all treated plots except for limed soils. An increase of 1.3 pH units was initially obtained by liming, but the pH dropped to about 0.9 unit after 20 months of incubation. In addition, total P in arable layer remained fairly constant. According to the recoveries of P added referring to 4.3 P 100 g^‐1, half‐life of a single P dose was estimated at 31 months for a P fertilized soil and almost threefold delay for combination of P fertilization and lime application, which was obtained from the following equation:     

No‐tillage corn response to placement of fertilizer nitrogen,phosphorus, and potassium

Abstract

Fertilizer placement for corn (Zea mays L.) has been a major concern for no‐tillage production systems. This 3‐yr study (1994 to 1996) evaluated fertilizer phosphorus (P) or potassium (K) rates and placement for no‐tillage corn on farmers’ fields. There were two sites for each experiment involving fertilizer P or K. Treatments consisted ofthe following fertilizer rates: 0,19,and 39 kg P ha^‐1 or 0, 51, and 102 kg K ha^‐I. The fertilizer was broadcast or added as a subsurface band 5 cm beside and 5 cm below the seed at planting. Early plant growth, nutrient concentrations, and grain yields were measured. At the initiation of the study, soil test levels for P and K at the 0–1 5 cm depths ranged from optimum (medium) to very high across sites. Effects of added fertilizer and placement on early plant growth and nutrient concentrations were inconsistent. Added fertilizer had a significant effect on grain yields in two of twelve site‐years. Therefore, on no‐tillage soils with high fertility, nutrient addition, and placement affected early plant growth and nutrient utilization, but had limited effect on grain yield. Consequently, crop responses to the additions of single element P or K fertilizers under no‐tillage practices and high testing soils may not result in grain yield advantages for corn producers in the Northern cornbelt regardless of placement method.     

Yield and phosphorus efficiency of some lowland rice varieties at different levels of soil‐available phosphorus

Abstract

A field experiment was conducted on an Aerie Haplaquept soil to study the effect of phosphorus (P) deficiency in soil on the P nutrition and yield of five modern varieties of rice, viz., Purbachi, BR1, BR3, BR14, and BR29, popular with the rice farmers of Bangladesh. Soil‐available P in the different plots of the experimental field varied widely, from 2.8 to 16.4 ppm. This plot to plot variation in soil‐available P content resulted from differences in the total amounts (0 to 480 kg ha^‐1) of P the plots had received over a period of 8 years in a long‐term P fertilizer trial conducted previously in the same field. Phosphorus deficiency in soil drastically reduced the grain yield of all the rice varieties. In severely P deficient plots, where soil‐available P was around 3 ppm, the yield was less than 1 ton ha^‐1 while in plots containing an adequate P level, i.e., >6 ppm, the yield was more than 41 ha^‐1. Rice yield increased linearly with an increase in soil P content up to 6 ppm, and the highest grain yield for any variety, obtained at 6–7 ppm of soil‐available P leveled off at this point. Soil P deficiency not only decreased rice yield severely but also decreased P content in straw and grain drastically. However, differences among rice varieties were noted in P nutrition, particularly at low soil P levels. The rice varieties differed markedly also in respect of internal P efficiency. The BR29 showed the highest internal P efficiency both at low and high soil P levels. In all the rice varieties, internal P efficiency decreased with an increase in soil P levels.     

Evaluation of soil extractants to estimate available micronutrients under wheat‐field conditions

Abstract

Three extracting reagents were evaluated by correlation analyses to provide the best index of Zn, Cu, Mn and Fe availability to wheat (Triticum aestivum L.) plants growing under open field conditions. Twenty one soils were selected to obtain the widest range in properties of soils of the land wheat cultivated. The magnitude of the extractive power varied in the following order: 6NHCl ? EDTA + NH₄OA_C, pH4.65 > DTPA‐TEA, pH 7.3. The mild extractants, EDTA and DTPA, gave the same order of removal of micronutrients being Zn < Cu < Fe < Mn. The acid extractant was on the contrast more effective on Cu and Fe with respect to Zn and Mn, respectively. Wheat concentrations of Zn, Mn and Fe were significantly correlated to soil micronutrients. Highly significant relationships were found for Zn extracted by DTPA solution (r = 0.737***) and for Mn and Fe extracted by EDTA solution (r = 0.710*** and r = 0.564**). Plant Zn and Mn were also well predicted by the acid extraction. The absence of correlation for plant Cu vs. soil Cu occurred probably because of wheat concentrations almost constant, ranging from 5.0 to 8.0 mg/kg.