短期连续光照下水培生菜品质指标变化及其关联性分析

以温室自然光照下水培20 d的生菜为试验材料, 研究其在72 h连续光照下叶片和叶柄中硝酸盐、可溶性糖及Vc含量的变化规律及其关联性。结果表明: 在72 h的连续光照下, 生菜叶片和叶柄中的硝酸盐含量大幅度降低, 但在不同光照时期降低速度各不相同, 叶片和叶柄中的硝酸盐含量分别在连续光照24 h和48 h后趋向于稳定; 而可溶性糖和Vc含量以近似恒定的速度快速提高, 其增速在72 h后并未表现出变缓趋势。可溶性糖在叶片和叶柄中的分布无显著差异, 但叶片中的Vc含量显著高于叶柄。无论是在叶片还是叶柄中, 硝酸盐含量与可溶性糖及Vc含量均呈极显著负相关。短期连续光照可实现生菜低硝酸盐与高可溶性糖及Vc的统一, 显著提高了生菜的综合品质。     

LED光源R／B对叶用莴苣生理性状及品质的影响

研究不同红／蓝（R／B）光强配比对叶用莴苣（LactucasativaL．）生理性状及品质方面的影响,以便为新型人工光源在莴苣生产上的应用提供理论依据。试验利用波长为660nm（±20nm）红光与450nm（±20nm）蓝光发光二极管（LightEmittingDiode,LED）作为人工光源,设置3个R／B试验处理,分别为LED1（R／B=10）、LED2（R／B=8）、LED3（R／B=6）,以1个荧光灯处理为对照（CK）,选用水耕栽培模式在不同光照环境下培育叶用莴苣30d。结果表明：660nm红光和450nm蓝光组合可以有效提高莴苣的光能利用率,促进光合作用;适宜的红蓝光比例能有效增加植物Vc含量,并降低硝酸盐含量。其中,处理LED2（R／B=8）的叶用莴苣净光合速率及Vc含量明显高于其他试验处理,而硝酸盐含量最低,表现出良好的生理和品质特性,因此,R／B=8相对更适宜叶用莴苣的生长发育。     

Growth of rice plants under red light with or without supplemental blue light

Abstract

The effects of blue light supplementation to red light on growth, morphology and N utilization in rice plants (Oryza sativa L. cv. Sasanishiki and Nipponbare) were investigated. Plants were grown under two light quality treatments, red light alone (R) or red light supplemented with blue light (RB; red/blue-light photosynthetic photon flux density [PPFD] ratio was 4/1), at 380 mol m^?2 s^?1 PPFD. The biomass production of both cultivars grown under RB conditions was higher than that of plants grown under R conditions. This enhancement of biomass production was caused by an increase in the net assimilation rate (NAR). The higher NAR was associated with a higher leaf N content per leaf area at the whole-plant level, which was accompanied by higher contents of the key components of photosynthesis, including Rubisco and chlorophyll. In Sasanishiki, preferential biomass investment in leaf blades and expansion of wider and thinner leaves also contributed to the enhancement of biomass production. These morphological changes in the leaves were not observed in Nipponbare. Both the changes in physiological characteristics, including leaf photosynthesis, and the changes in morphological characteristics, including leaf development, contributed to the enhancement of biomass production under RB conditions, although the extent of these changes differed between the two cultivars.     

Accumulation and Primary Metabolism of Nitrate in Lettuce (Lactuca Sativa L. Var. Youmaicai) Grown Under Three Different Light Sources

Light-emitting diodes (LEDs) hold great potential as electric light source for leafy vegetable production in controlled environment. However, most of LED lights in vegetable cultivation were only combined with red and blue LEDs, and the spectral composition was oversample. Previous studies reported that light spectral composition could influence nutritional quality of vegetables, e.g., nitrate content. In order to better understand the influence of spectral composition on nitrate accumulation of leafy vegetables grown in closed-type system. The nitrate content, nitrate primary metabolism of 35-day-old lettuce (Lactuca sativa L. Var. youmaicai) in vivo and its biomass were investigated. The results showed that cool white fluorescent light (T5) and high-pressure sodium light (HPS) with wide spectrum significantly reduced nitrate content in lettuce than red and blue combination LED light (RB-LED) treatment. Our findings demonstrated that a proper optimizing procedure of light spectrum with supplemental other wavebands in RB-LEDs is necessary for reducing nitrate accumulation of lettuce.     

LED红蓝光对不同品种水培生菜的影响效果对比

选取9个不同叶色和叶形的生菜品种,以红蓝光比为7︰1的复合光（RBL）为光源,在植物工厂内对比白光（WL）进行试验,以探究红蓝复合光（RBL）对不同品种生菜（Lactuca sativa L.）的响应差异性及影响效果。结果表明,相比于白光（WL）处理,红蓝光（RBL）促进了绿色散叶生菜品种的株高相对生长速率（P＜0.05）,但是抑制了其长势过旺;降低了紫色生菜品种后期叶绿素含量;提高了半结球和散叶生菜品种的净光合速率、蒸腾速率和气孔导度（P＜0.05）;抑制了所有品种生菜PSⅡ的最大光化学效率;提高了散叶生菜品种的ETR值（P＜0.05）;提高了半结球生菜的可溶性固形物含量和可溶性糖含量（P＜0.05）;提高了绿色散生生菜品种的维生素C含量、产量和干物率（P＜0.05）。总之,红蓝光（RBL）对不同品种生菜生长发育及光合荧光特性的影响差异性主要与叶片离散程度相关,对绿色生菜品种品质和产量的提升效果普遍优于紫色生菜品种。     

补光时间及光质对温室甜椒生长及产量品质的影响

为确定LED补光调节温室甜椒(Capsium annuum L.)产量品质的最优光质及补光时长,以甜椒品种"奥黛丽"为试材,设置红光(R)和蓝光(B)组合(灯珠个数比)2∶1(2R1B)、4∶1(4R1B)、8∶1(8R1B)3种光质,2 (18:00-20:00)、4(18:00-22:00)和8 h(18:00-02:00)3个补光时间;以不补光为对照,研究补光时间及光质对甜椒生长、产量及品质的影响。结果表明:光质与补光时间对甜椒植株的影响有较大差异,且两者交互作用显著。2R1B光质补光2 h,冠层宽、可溶性蛋白含量、可溶性糖含量、维生素C含量最大,显著高于对照;补光4 h茎粗最大,显著高于对照;补光8 h,硝酸盐含量最低,比对照降低19.4%。4R1B光质补光2 h,株高、根鲜质量、地上部、根及全株干质量最高,显著高于对照;补光8 h,叶面积、单株果实数及单株产量最高,显著高于对照。8R1B光质补光2 h,叶绿素相对含量SPAD(soil and plant analyzer development)值及糖酸比最高,显著高于对照;补光8 h,地上部及全株鲜质量最高,显著高于对照。利用隶属函数法对所有补光处理的产量品质指标及用电量等进行综合评价,由综合得分排序得出,最有利于甜椒栽培的前3种补光组合依次为光质8R1B补光2 h、光质4R1B补光8 h和光质8R1B补光8 h。因此,光质8R1B补光2 h可以作为最适宜当地甜椒栽培的补光组合,该研究结果为日光温室甜椒种植的光调控技术提供理论参考。     

Study of the Transfer Coefficient of Cadmium and Lead in Ryegrass and Lettuce

Abstract

The aim of study was to find the correlation between the concentration of the total amount of heavy metals of soils and that of plants because it shows which elements can be accumulated by different plants. The transfer coefficient is the metal concentration in plant tissues aboveground divided by the total metal concentration of soil. Pot experiments were conducted under greenhouse conditions. The total lead (Pb) content (about 21 mg · kg^?1 soil) of soils was higher than the cadmium (Cd) content (about 0.21 mg · kg^?1 soil). The Cd concentration of lettuce (averaging 0.93 mg · kg^?1) was higher than that of ryegrass (averaging 0.20 mg · kg^?1). The transfer coefficient of Cd was lower in ryegrass (averaging 0.95) than in lettuce (4.47). In this experiment, the concentration of Cd was almost five times higher in the four‐leaf lettuce than the Cd content of soil. The transfer coefficient of Pb was generally 0.064 in both plants.     

弱光条件下光质和光周期对水培生菜生长与品质的影响

在弱光条件的人工光植物生长室中,设置两种不同红蓝光比例光质和两种不同光周期,水培盆栽红叶生菜（R）、绿叶生菜（G）和紫叶生菜（P）,采收后测定3种生菜的生物量指标以及抗氧化物、可溶性糖等营养品质指标,以研究弱光条件下光质和光周期对3种叶色水培生菜产量和品质的影响。结果表明：（1）生菜产量主要受光周期影响,营养品质指标受光质和光周期的综合影响。光照时间延长显著增加了3种生菜叶面积。绿叶生菜和红叶生菜单位叶面积鲜重在不同光质及光周期下无显著差异。紫叶生菜单位叶面积鲜重对光周期变化的反应则因光质环境而异。延长光周期使3种叶色生菜地上部鲜重提高一倍多,但3种生菜地上部鲜重受光质影响不显著。（2）延长光照时长对3种生菜营养品质的影响因光质而异。在红蓝1:2光质下,延长光照时长降低了绿叶生菜总酚和类黄酮相对含量以及抗坏血酸和可溶性蛋白含量,提高了红叶生菜花青素和可溶性糖含量,但降低了抗坏血酸和可溶性蛋白含量,紫叶生菜总酚相对含量得到提高,但抗坏血酸含量显著降低。在红蓝2:1光质下,延长光照时长显著提高了绿叶生菜总酚和类黄酮相对含量,抗坏血酸含量有所降低,红叶生菜总酚和类黄酮相对含量、抗坏血酸、可溶性糖和可溶性蛋白含量均显著降低,紫叶生菜总酚含量显著提高。说明在弱光条件下延长光照时长可以显著提高3种不同叶色生菜产量。在红蓝1:2光质下,延长光照时长使红叶生菜的花青素含量和可溶性糖含量得到显著提高。在红蓝2:1光质下,延长光照时长可以提高绿叶生菜和紫叶生菜营养品质。     

营养液浓度对水培生菜生长和硝酸盐积累的影响

该文研究了4种营养液浓度处理(山崎生菜配方的1/4(1/4s)、1/2(1/2s)、1(1s)和2(2s)单位)对水培生菜(品种为“弘农”和“绿领”)生长、硝酸盐积累和品质的影响。结果表明，1/2单位营养液浓度处理下生菜地上部和根的鲜重、叶长和叶宽最大，而2个单位营养液浓度处理下上述指标最小。不同营养液浓度处理下叶片硝酸盐积累的高低顺序为2s>1s>1/2s>1/4s，2个单位营养液浓度处理下生菜生长发育后期叶片中的SOD活性和MDA含量最高。对采收时生菜品质的分析表明，生菜体内的硝酸盐含量随着营养液浓度的增加而迅速上升，2个单位营养液浓度处理下生菜叶片中硝酸盐、可溶性糖和可溶性蛋白质R 含量最高。综合考虑产量和品质，采用1/2单位的山崎生菜配方是适合生菜水培的营养液配方。     

昼夜连续照射LED红蓝光对不同品种生菜生长和品质的影响

为探究生菜对全生长期连续光照的响应及品种差异性,在密闭式植物工厂内,以LED红蓝光为光源研究了水培条件下常规光照（12h/12h,NL）与连续光照（24h/0h,CL）对5种生菜生长和品质的影响。结果表明：与常规光照（NL）处理相比,连续光照（CL）处理显著提高了除“大卫”外其它4种生菜的地上部鲜重,其中“绿罗”生菜增加幅度最高。生长初期连续光照（CL）处理下5种生菜叶片叶绿素含量显著高于常规光照（NL）处理,但在生长后期,5种生菜两个处理下的叶绿素含量均无显著差异。与常规光照处理相比,连续光照处理显著提高了5种生菜可溶性糖、总酚和类黄酮含量,以及其中2种紫叶生菜的花青素含量。除“绿罗”外,其它4个品种连续光照处理下的抗坏血酸总量均略有或显著升高,其中主要提高了还原型抗坏血酸的含量,对脱氢抗坏血酸含量的影响不显著。总之,全生长期连续光照能显著提高生菜的产量,促进可溶性糖和抗氧化物质合成;5个品种生菜对连续光照的适应性存在差异,其中“绿罗”的适应性最强;全生长期连续光照运用于植物工厂生菜生产对提高产量和品质的潜力较大。     

Effect of High‐Molybdenum Compost on Soils and the Growth of Lettuce and Barley

A pot experiment evaluated the growth of lettuce (Lactuca sativa L.) and barley (Hordeum vulgar) and accumulation of molybdenum (Mo) in plants and soils following amendments of Mo compost (1.0 g kg^?1) to a Truro sandy loam. The treatments consisted of 0 (control), 12.5, 25, and 50% Mo compost by volume. The Mo compost did not affect dry‐matter yield (DMY) up to 25% compost, but DMY decreased at the 50% compost treatment. The 50% compost treatments increased the soil pH an average of 0.5 units and increased the nitric acid (HNO₃)–extractable Mo to 150 mg kg^?1 and diethylenetriaminepentaacetic acid (DTPA)–extractable Mo to 100 mg kg^?1 in the growth medium; the same treatment increased tissue Mo concentration to 569 and 478 mg kg^?1 in the lettuce and barley, respectively. Plants grown in the 25% compost produced about 55 mg kg^?1 of total Mo in the growth medium; this resulted in tissue Mo concentration of 348 mg kg^?1 in lettuce and 274 mg kg^?1 in barley without any phytotoxicity. Our results suggested that 55 mg Mo kg^?1 soil would be an appropriate limit for Mo loading of soil developed from compost additions, a value which is presently greater than the Canadian Council for Ministers of the Environment (CCME) Guidelines for the use of type B compost in Canada.     

营养液浓度对水培生菜生长和硝酸盐积累的影响

该文研究了4种营养液浓度处理(山崎生菜配方的1/4(1/4s)、1/2(1/2s)、1(1s)和2(2s)单位)对水培生菜(品种为“弘农”和“绿领”)生长、硝酸盐积累和品质的影响。结果表明,1/2单位营养液浓度处理下生菜地上部和根的鲜重、叶长和叶宽最大,而2个单位营养液浓度处理下上述指标最小。不同营养液浓度处理下叶片硝酸盐积累的高低顺序为2s>1s>1/2s>1/4s,2个单位营养液浓度处理下生菜生长发育后期叶片中的SOD活性和M DA含量最高。对采收时生菜品质的分析表明,生菜体内的硝酸盐含量随着营养液浓度的增加而迅速上升,2个单位营养液浓度处理下生菜叶片中硝酸盐、可溶性糖和可溶性蛋白质R含量最高。综合考虑产量和品质,采用1/2单位的山崎生菜配方是适合生菜水培的营养液配方。     

Zinc accumulation in lettuce cultivars grown with organic or chemical based nutritional regimes

This study determined the potential to increase Zn density of lettuce (Lactuca sativa L.) through cultivar selection and nutrient management. Organic fertilizer and Hoagland and Arnon no.1 solution factored with three zinc (Zn) levels provided as zinc sulfate (ZnSO₄) were the fertilizer regimes in a greenhouse experiment. Modern cultivars had a 32% higher fresh head weight than heritage cultivars, but each accumulated the same Zn concentration (65 mg kg^?1 dry wt). Butterhead phenotypes had a 38% lower yield than loose-leaf and had the highest Zn concentration (78 mg kg^?1 dry wt) followed by romaine (66 mg kg^?1 dry wt) and loose-leaf (53 mg kg^?1 dry wt). Concentration of Zn did not differ between fertility regimes, being about 66 mg kg^?1 dry wt with each regime. Differences in Zn concentrations were significant among individual cultivars with ranges from 42 mg g^?1 dry wt to 91 mg kg^?1 dry wt. ‘Tom Thumb’, ‘Adriana’, ‘Claremont’, and ‘Focea’ were the top in cultivar ranking, with mean Zn concentration of 63 mg kg^?1 dry wt. The results signify that selection of cultivars may be utilized to increase Zn accumulation in lettuce but that nutritional regimes had little effect on accumulation.     

LED光源不同R/B处理对甘薯组培苗品质及节能效果的影响

采用红色LED(660±20nm)和蓝色LED(450±20nm)组合制成的LED灯管作为组培人工光源,研究光照度为35μmol.m-2.s-1时,红蓝光质比(R/B)分别为4、6、8、10的光环境培养条件下甘薯组培苗的生长情况,以相同光照度的荧光灯为对照,培育甘薯组培苗28d。结果表明:660nm红光和450nm蓝光组合可以有效抑制植物徒长,有降低地上部分含水率和提高根冠比的作用,荧光灯下生长的植株地上鲜重、叶片含水率和株高均最大,但是植株徒长,干物质积累不良。高的R/B处理能提高植株高度和根冠比,增大地下鲜重,降低地上含水率,有利于干物质积累;R/B为8时甘薯组培苗的地下鲜重和根冠比均最大;R/B变化对叶片光合色素含量没有显著影响。另外,LED光源的电能消耗与R/B值呈线性增加关系,并比荧光灯节能27.6%~48.0%。     

Copper/Zinc Bioaccumulation and the Effect of Phytotoxicity on the Growth of Lettuce (<Emphasis Type="Italic">Lactuca sativa</Emphasis> L.) in Non-contaminated,Metal-Contaminated and Swine Manure-Enriched Soils

Copper/zinc bioaccumulation and the effect of phytotoxicity on the growth of lettuce (Lactuca sativa L.) were studied in plastic vessels containing (i) non-contaminated soil, (ii) copper-contaminated soils at concentrations of 75.0 and 125.0 mg kg^?1, (iii) zinc-contaminated soils at concentrations of 1200 and 2400 mg kg^?1, and (iv) soil enriched with swine manure. Copper and zinc concentrations in lettuce leaves were determined by flame atomic absorption spectrometry during 42 days of growth. Copper concentrations from 0.92 to 13.06 mg kg^?1 were found in lettuce leaves grown in copper-contaminated soils and zinc concentrations from 58.13 to 177.85 mg kg^?1 were found in lettuce leaves grown in zinc-contaminated soils. Copper and zinc concentrations in lettuce leaves grown in swine manure-enriched soils ranged from 0.82 to 8.33 and 0.68 to 13.27 mg kg^?1, respectively. Copper and zinc bioaccumulation caused a decrease in lettuce growth in metal-contaminated soils and an increase in phytotoxicity effects when compared to growth in non-contaminated and manure-enriched soils. These findings were confirmed by measuring leaf areas and biomasses. Copper was less toxic to lettuce than zinc due to the different concentrations in the soil. Lettuce growth and development was better in the swine manure-enriched soil than non-contaminated soil, which indicates that swine manure is a safe agricultural biofertilizer when used in appropriate amounts to avoid metal bioaccumulation in soil and plants.     

Functional and Structural Changes associated with Cadmium in Mustard Plant: Effect of Applied Sulphur

Abstract

The effect of cadmium (Cd) and sulphur (S) on dry weight, biochemical parameters and anatomical features of mustard (Brassica campestris L. cv. Pusa Bold) plant was investigated in a pot culture experiment using Cd (25, 50, and 100 mg kg^?1 of soil), S (40 mg kg^?1 of soil), and the combination of Cd+S (25+40 mg kg^?1 of soil, 50+40 mg kg^?1 of soil, and 100+40 mg kg^?1 of soil). Sulphur treatment was given at sowing and Cd treatment was given when seedlings were fully established. Observations were recorded at the flowering stage. A significant and antagonistic interaction of Cd and S was observed. Compared to the control, leaf dry weight, total chlorophyll content, sugar content, nitrate reductase activity, and protein content decreased significantly with each Cd treatment, whereas the reverse was observed with S treatment. Combined treatments of Cd+S also reduced these parameters, but this reduction was less than the one observed with Cd treatments alone. However, nitrate accumulation in the leaves was 2.35 times higher with treatment of 100 mg Cd+40 mg S kg^?1 of soil than in the controls, whereas it was 3.5 times higher with Cd (100 mg kg^?1 of soil) alone. The relative proportion of vasculature in the stem, stoma length and width, and stomata length and width were inhibited with Cd treatments, whereas the combined treatments mitigated the adverse effect caused by Cd. Thus, S could alleviate the Cd induced impairment of biochemical and anatomical features of the plant and the enhancement of nitrate accumulation in the leaves.     

荧光粉激发型远红光LED植物灯对番茄生长的影响研究

为探究远红光对植物生长的影响,采用“燃烧法+高温二次烧结”的方法制备了ZnGa₂O₄:Cr³⁺,Ge⁴⁺,Li⁺(ZGO:Cr³⁺,Ge⁴⁺,Li⁺)荧光粉,将自制的荧光粉材料涂覆在410 nm的蓝紫光LED芯片上,封装成发射主峰位于710 nm左右的LED远红光器件,并进一步组装成LED植物生长灯作为直接光源。以番茄(Solanum lycopersicum)品种红矮生为试材,白光(W)为对照(CK),设置3个处理:T1:红蓝组合光(1R1B)加远红光(光质比为3:1);T2:红蓝组合光(1R1B);T3:白光加远红光(光质比为3:1),研究红蓝组合光(1R1B)和远红光对番茄生理周期、农艺性状、光合色素和番茄果实主要品质的影响。结果表明,与CK相比,T1处理可显著缩短番茄生理周期,T1、T2和T3处理对番茄农艺性状中的株高有抑制作用。添加远红光降低了光合色素的含量,但提高了番茄果实中可溶性糖、番茄红素和维生素C的含量,特别是在红蓝组合光与远红光的共同作用下,可溶性糖含量提高效果最明显,达到CK的3.3倍。添加远红光能够促进维生素C和番茄红素含量增加;红蓝组合光则有利于番茄红素的积累。综上,采用ZGO:Cr³⁺,Ge⁴⁺,Li⁺远红光荧光粉激发型LED植物灯能调控番茄开花时间,缩短其生理周期,提高番茄果实品质。本研究结果为提高番茄以及其他园艺作物的品质提供了一种新的光照手段,具有广阔的应用前景。     

LED绿光对生菜生长和品质的影响

在全人工光型植物工厂内以水培奶油生菜（Lactuca sativa L.）为试验材料,运用光质及光强可调节的LED植物生长灯,以8：00-24：00照射强度为200μmol·m-2·s-1的白光为基础光（对照W）,依次以强度为50（WG50）、100（WG100）、150μmol·m?2·s?1（WG150）的绿光取代相等强度的白光照射生菜,并测定生菜叶片生物量、形态、叶片数以及叶绿素、硝态氮、维生素C、可溶性糖、粗纤维和淀粉的含量,以探究绿光对生菜生长及品质的影响,为LED绿光的供光策略提供参考。结果表明：（1）在50μmol·m-2·s-1的绿光处理下,生菜地上部叶绿素含量显著高于对照,地上部干重较对照提高3.69%。（2）随着绿光强度的增加和基础白光强度的减弱,生菜地上部可溶性糖和维生素C含量均呈现先升高后降低的趋势,二者均在WG50处理下达到最高,并显著高于对照（P＜0.05）,而在WG150处理下达到最低,并显著低于对照（P＜0.05）。（3）与对照相比,绿光处理均降低了生菜叶片中淀粉、粗纤维以及硝酸盐的含量,其中硝酸盐含量在WG50处理下达到最低。说明50μmol·m-2·s-1强度的绿光有促进生菜干物质积累以及提升生菜品质的作用。     

Effects of short-term treatment with various light intensities and hydroponic solutions on nitrate concentration of lettuce

Abstract

A glasshouse experiment was conducted to investigate the effects of five days’ treatment before harvest with various light intensities and seven hydroponic solutions on nitrate reduction in lettuce. The results showed that SPAD of new and expanded leaves were elevated by most of the hydroponic solutions under shading compared with the full nutrient solution, while no effect was demonstrated under non-shading. Additionally, lettuces under non-shading presented slightly higher leaf SPAD than those under shading. Nitrate concentrations of lettuce new leaves, expanded leaves and petiole were lowered significantly by all hydroponic solutions under shading and non-shading conditions compared with the full nutrient solution. Under non-shading, the efficiencies of seven hydroponic solutions varied largely, but no efficiency differences were showed under shading. N-free solution, 0.75 mM potassium sulphate solution and 5×10^?5 mM ammonium molybdate solution functioned more efficiently in reducing nitrate concentration both in expanded leaves and petiole of lettuce before harvest. In conclusion, proper light intensity is a key important factor that determines the efficiencies of nitrogen-free hydroponic solution treatments in lowering nitrate concentrations in leaf blades and petioles of lettuce before harvest.     

Heavy Metal Content in Lettuce Plants Grown in Biosolids Compost

The objective of this work was to evaluate the affects of the application of composted biosolids on the accumulation of heavy metals (Cd, Cu, Ni, Pb and Zn) in lettuce leaves. Pots containing different proportions (0 to 100%) of composted biosolids were used to grow lettuce plants under greenhouse conditions. Dry and fresh weight, leaf area and Cd, Cu, Ni, Pb and Zn uptake were determined after harvest. It was found that the dry and fresh matter productions of the plants were significantly lower in the control treatment. The addition of composted biosolids caused a 20 and 40% increase in biomass accumulation. Cd and Pb concentrations in leaves were below detection limits (0.05 mg kg^?1) in all treatments. Zn concentration in leaves increases as compost proportion decreases, ranging from 57.2 to 80.4 mg kg^?1. Composted biosolids application increased the Cu and Ni plant concentrations, ranging from 5.1 to 9.8 mg Cu kg^?1 and 2.3 to 3.7 mg Ni kg^?1. In all treatments the proportions of heavy metals in plants were below the international standards of toxicity. The results allow us to suggest that, in short-term applications, composted biosolids could be used as soil amendment for lettuce production, without toxic effects in the chemical composition of the plant.