Nitrogen nutrition and use efficiency in rice as influenced by crop establishment methods,cyanobacterial and phosphate solubilizing bacterial consortia and zinc fertilization

ABSTRACT

A field experiment was conducted at the ICAR-Indian Agricultural Research Institute, New Delhi during wet season of 2013 and 2014, involving three crop establishment methods (CEMs) viz. puddled transplanted rice (PTR), system of rice intensification (SRI), and aerobic rice system (ARS). Three rates of nitrogen (N) and phosphorus (P) application (0%, 75%, and 100% of recommended dose of N and P) (RDN) (120 kg N ha^–1 and 25.8 kg P ha^–1) and two sources of N and P (fertilizer and cyanobacterial-bacterial inoculation) were tested with and without zinc (Zn) fertilization in all CEMs. The concentration of N in PTR and SRI was significantly higher than ARS with higher uptake of 10.3 and 11.1 kg ha^–1 in PTR and SRI over ARS. Treatment with 100% RDN led to significantly higher N availability and uptake than 75% RDN and absolute control, i.e. 14.5 and 32.0 kg ha^–1, respectively. Application of Anabaena-Pseudomonas biofilm formulation (MC2) in conjunction with 75% RDN increased total uptake of N by 12.7 kg ha^–1 compared to 75% RDN only. A positive correlation was found between N concentration and acetylene reductase activity at 70 days after sowing (DAS) (R² = 0.52) and 100 DAS (R² = 0.38) based on mean of 2 years study. Zn fertilization significantly increased N concentration in rice straw and milled rice irrespective of the crop establishment methods. Our study signifies the importance of microbial inoculation, optimal N fertilization along with SRI and PTR as more effective crop establishment methods for deriving greater benefits in terms of N nutrition in rice.     

水稻强化栽培对稻田土壤生物学特性的影响

采用田间试验,对强化栽培(SRI)和常规水作(TF)条件下稻田土壤微生物数量、微生物量、土壤酶活性及相关养分含量进行了研究。结果表明:与常规水作相比,强化栽培增加了土壤细菌、放线菌、真菌数量,分别提高了53.1%～173.8%、61.7%～229.4%、10.0%～55.9%,统计差异显著(p<0.05)。强化栽培土壤微生物量碳、微生物量氮比常规水作提高了12.2%～43.6%,22.7%～175.4%,统计差异显著(p<0.05)。与常规水作比较,强化栽培提高了分蘖期土壤脲酶、碱性磷酸酶、转化酶、过氧化氢酶活性,分别为12.6%、30.0%、15.1%、13.8%;强化栽培也显著增加了土壤碱解氮含量,但有效磷含量差异不显著。     

Comparisons of Yield,Water Use Efficiency,and Soil Microbial Biomass as Affected by the System of Rice Intensification

To compare the effects of the system of rice intensification (SRI) on yield, water use efficiency, and microbial biomass in associated rice soils, a field experiment was conducted in 2004 at the Agriculture Experimental Farm of Zhejiang University in Zhejiang Province, China. The treatments evaluated were traditional flooding (TF) vs. SRI cultivation methods. Grain yield in the SRI treatment was 26.4% greater than that in the TF treatment, reducing water use by 461.5 mm. Compared to TF, SRI increased water use efficiency by 91.3% and irrigation water use efficiency by 194.9%. Soil microbial indicators during the rice‐growing season also diverged between TF and SRI. Microbial biomass C (MBC) was in the range of 101–196 mg kg^?1 for TF vs. 113–224 mg kg^?1 for SRI; microbial biomass N (MBN) was in the range of 14–33 mg kg^?1 for TF vs. 28–53 mg kg^?1 in SRI. Compared to TF, SRI significantly increased both MBC and MBN, regardless of sampling date.     

施用磷和锌对植株体中锌营养及代谢的影响

A solution culture experiment was conducted to investigate the growth,the accumulation and translocation of Zn,and the metabolic changes of 24 days old plants of corn and wheat with the varied suply of phosphorus(0,0.12,0.6 and 3.0mmol L^-1)and zinc (0.1 and 2.0umol L^-1) under controlled environmental conditions.The results showed the highest dry matter production of both corn and wheat under the moder ate combination of phosphorus(0.6mmol L^-1) and zinc(2.0 umolL^-1) as compared with other imbalance applications of phosphorus and zinc.Excessive P supply significantly inhibited the translocation of Zn from roots of corn to the aboveground part,thus decreasing the content of Zn in the shoots.Application of 3.0 mmolL^-1 P could also reduce the water-soluble Zn in plant tissues,leading to an increase in the cell plasma membrane permeability,a decrease in the dehydrogenase activity in roots and the activity of nitrate reductase in leaves,and a decline in the uptake of nitrate by plants.A similar decrease occurred in superoxide dismutase(SOD) and plasma membrane adenosine triphosphatase(ATPase)activity in Zn-deficient plants.But,with increasing P supply the activity of ATP ase in both corn and wheat increased and reached the maximum at the P-supplying level of 3.0 mmolL^-1.Similar to the effect of high P supply.no or low P(0.12mmolL^-1) supply could be detrimental to dry matter production and physiological functioning of the plants.Corn plants showed a more significant response to the imbalance supply of P and Zn than wheat plants.The possible physiological and biochemical mechanism of phosphorus-zinc antagonistic interaction in corn and wheat might be attributed to decrease in physiological availability and activation of Zn.     

Interaction effect of nitrogen,phosphorus, and zinc fertilization on growth,yield, and nutrient contents of aromatic rice varieties

Abstract

Studies on nutrient interactions in aromatic rice are needed for proper understanding of impact of imbalanced use of nutrients in the era of multi and micro-nutrient deficiencies. A pot experiment was conducted during the rainy/wet season (June–October) of 2013 at New Delhi, to study the interaction effects of two levels each of nitrogen (N) (0 and 120?kg?ha^?1), phosphorus (P; 0 and 25.8?kg?ha^?1), and zinc (Zn; 0 and 5?kg?ha^?1) in two aromatic rice (Oryza sativa L.) varieties, viz. Pusa Rice Hybrid 10 and Pusa Basmati 1121. Application of N, P, and Zn resulted in increase of dry matter (0.91, 0.32, and 0.24?g plant^?1, respectively) 60?days after sowing (DAS) and grain yield of rice (3.68, 1.67, and 1.17?g plant^?1). The increase in yield of rice owing to N application was relatively higher by 0.98, 0.22, and 1.05?g plant^?1, respectively, when either P or Zn or both were applied with N than alone application of N, indicating synergetic effect of P and Zn application with N. The higher concentration and uptake of K in grain (0.25% vs 0.10%) and straw (1.32% vs 0.94%) were observed in the treatment received N than no N, though K was applied uniformly in all the treatments. It indicates positive interaction of N and K. The higher uptake of P in grain and straw was observed when P was applied with N and Zn (3.34 and 2.15?mg plant^?1), or with N (3.26 and 2.11?mg plant^?1) signifying positive effect of N on P uptake in rice.     

System of rice intensification in promising rice hybrids in north-western Himalayas: crop and water productivity,quality, and economic profitability

System of Rice Intensification (SRI) has spread as an innovation of rice cultivation that can produce higher crop yields and conserve seed and water resources. The SRI innovation is also gaining popularity in north-western (NW) Himalayas on one hand and hybrid rice technology on the other in the region. Moreover, rice productivity in NW Himalayas is quite low owing to the use of low-yielding germplasm and poor crop management. Thus, SRI principles coupled with hybrid rice technology seems to be a boon to boost rice productivity in the irrigated ecosystem of wet-temperate NW Himalayas well known for rice cultivation. Therefore, comparative performance of promising rice hybrids under SRI was assessed at three locations in wet-temperate NW Himalayas (India) using nine promising rice hybrids including state-recommended rice hybrid “Arize–6129” as check cultivar. It was revealed that various hybrids differed significantly w.r.t. days to 50% flowering, days to 75% maturity, plant height, tillers hill^?1, panicles hill^?1, panicles m^?2, and panicle length. Highest number of panicles m^?2 (370) was observed in Arize–6129 followed by US–312, Bioseed–786, and NK–3325, respectively. Significantly longer panicles were observed in Dhanya–2366 followed by Arize–6129, US–312, Bioseed–786, NK–3325, and US–10, respectively. Arize–6129 resulted in significantly higher grain (75 q ha^?1) and straw yield (125 q ha^?1) followed by US–312, Dhanya–2366, NK–3325, PAC–801, US–10, Bioseed–786, Uday–111, and Uday–131, respectively. The production- and monetary-efficiency as well as gross and net returns and B:C ratio also followed the similar trend as that of crop productivity with significantly higher production– (67 kg ha^?1 day^?1) and monetary–efficiency (INR 608.4 ha^?1 day^?1), and net returns (INR 68138 ha^?1) and B:C ratio (3.66) in check cultivar “Arize–6129” over other rice hybrids. Higher grain productivity (49.5–75.0 q ha^?1), net returns (INR 39238–68138 ha^?1), and B:C ratio (2.53–3.66) in current study conclusively inferred that SRI coupled with hybrid rice technology can harness higher productivity and profitability. Protein content (8.30–8.45%) exhibited higher values under Bioseed–786 followed by NK–3325, UDAY–111, and Arize–6129; however, NPK uptake (grains, straw, total) was significantly highest in Arize–6129 followed by US–312, Dhanya–2366, and NK–3325, respectively. Total water productivity (6.4–9.75 kg ha^?1 mm^?1), irrigation water productivity (16.5–25 kg ha^?1 mm^?1), and economic water productivity (64.0–97.5 INR ha^?1 mm^?1) collectively followed the trend of Arize–6129 > US–312 > Dhanya–2366 > NK–3325 > US–10 > PAC–801 > Bioseed–786 > Uday–111 > Uday–131 in current study. Overall, Arize–6129, US–312, and Dhanya–2366 were proved as potential rice hybrids in terms of their higher crop and water productivity and economic profitability among above nine rice hybrids for their large-scale cultivation under SRI in wet-temperate NW Himalayas.     

Influences of past application rates of nitrogen and a catch crop on soil microbial communities between an intensive rotation

The aim of this study was to investigate influences of six-year past application rates of nitrogen and a catch crop, sweet corn (Zea mays L. ssp. Saccharata Sturt), on soil microbial community and diversity in a greenhouse-based intensive vegetable soil in eastern China. Soil electrical conductivity, pH, mineral nitrogen, phospholipid fatty acids (PLFA) profiles and carbon source utilization patterns under five annually past nitrogen rates (0, 348, 522, 696 and 870?kg?nitrogen?ha^?1) were evaluated after the establishment of sweet corn during 1–1.5-month fallow period over three-year tomato/cucumber/celery rotations. The past nitrogen application rates exerted significant effects on soil electrical conductivity, pH, nitrate-nitrogen, ammonium-nitrogen and carbon source utilization patterns, but not on PLFAs profiles. The sweet corn had a significant effect on soil chemical properties, total and actinobacterial PLFAs, but not on carbon source utilization patterns. Soil electrical conductivity, nitrate-nitrogen and the total PLFAs decreased whilst soil organic carbon, pH and the actinobacterial PLFAs increased after the establishment of sweet corn. Soil microbial functional diversity from carbon source utilization patterns and actinobacterial PLFAs were greatest after the establishment of sweet corn at a 60% of the conventional nitrogen rate (i.e. 522?kg?nitrogen?ha^?1). Soil electrical conductivity and ammonium-nitrogen were two key factors to determine carbon source utilization patterns, whilst soil pH was the key factor to determine PLFAs profiles. A combination of the catch crop sweet corn during summer fallow and a 60% of the conventional nitrogen rate is a sustainable pathway of utilizing greenhouse-based intensive vegetable soils in eastern China.     

减氮控磷稳钾施肥对水稻产量及养分积累的影响

氮、 磷用量偏大,钾肥用量不足不仅影响水稻的正常生长发育,而且导致养分利用率偏低。本文通过田间试验,研究减量施用氮、 磷肥,稳定钾肥投入对水稻产量、 养分积累量和肥料利用率的影响。试验设14个处理,每个处理重复2次。结果表明,氮钾、 磷钾、 氮磷钾配施处理的水稻秸秆生物量和籽粒产量均显著高于不施肥处理（P0.05）; 减氮控磷稳钾处理（N 225 kg/hm2、 P2O5 60 kg/hm2、 K2O 90 kg/hm2）与常规施肥处理相比（N 300 kg/hm2、 P2O5 150 kg/hm2、 K2O 60 kg/hm2）能显著增加水稻秸秆生物量（P0.05）,明显提高千粒重和籽粒产量; 试验还得出,减氮控磷稳钾处理分蘖期地上部氮、 钾含量和秸秆氮、 钾含量显著高于常规施肥处理（P0.05）; 收获期地上部氮、 钾的积累量和氮、 磷的表观利用率显著大于常规施肥处理（P0.05）。适当减少氮、 磷用量, 增加钾肥用量能改善氮、 钾营养状况,促进地上部干物质的积累,提高籽粒产量和氮、 磷表观利用率。N 196.2 kg/hm2、 P2O5 46.5 kg/hm2、 K2O 90 kg/hm2的配施方案具有实际推广应用价值。     

Effect of rates and sources of nitrogen on rice yield,nitrogen efficiency,and methane emission from irrigated rice cultivation

The agronomic benefits of manure application to increase rice production have been recognized, but the impact on global change has always been a controversial topic. This study was designed to determine the separate and combined effects of cattle manure (CM) and nitrogen (N) fertilizer on rice yield, N efficiency, and methane (CH₄) emissions from rice cultivation. A pot-scale experiment was conducted with four levels (0, 60, 120, and 180 kg ha^?1) of N from urea and two levels (120 and 180 kg ha^?1) of N from combination of urea and CM (Urea:CM = 60:60 and 60:120). Rice yield and physiological N efficiency were obtained using agronomic measurements. To determine the global warming potential (GWP) of each treatment, CH₄ emissions were measured throughout the rice-growing period. Grain yield (GY) was not significantly different between the treatments of 120 and 180 kg ha^?1 regardless of N source. However, both rates of CM treatments enhanced CH₄ emission and differences in GWP were significant. In conclusion, urea applied at 120 kg N ha^?1 was optimal for rice productivity and environmental impact (EI) despite CM played a crucial role in improving the N efficiency and total N in the soil after harvest.     

Effects of inoculation of cyanobacteria on nitrogen status and nutrition of rice (Oryza sativa L.) in an Entisol amended with chemical and organic sources of nitrogen

A field experiment was conducted with wetland rice (Oryza sativa cv. IR-36) in a sandy clay loam soil (Entisol) to study the effect of inoculation with a soil-based mixed culture of four diazotrophic cyanobacteria,Aulosira fertilissima, Nostoc muscorum, N. commune andAnabaena spp., on the N-flux in inorganic NH₄ ⁺+NO₃ ^–+ NO₂ ^–), easily oxidizable, hydrolysable and non-hydrolysable forms of N in soil during vegetative growth periods of the crop. Effects on grain and straw yield and N uptake by the crop were estimated. The effects of applying urea N and N as organic sources, viz.Sesbania aculeata, Neem (Azardirachta indica) cake and FYM, each at the rate of 40 kg N ha^–1, to the soil were also evaluated. Inoculation significantly increased the release of inorganic N, evidenced by its increased concentrations either in soil or in soil solution. However, such increases rarely exceeded even 4% of total N gained in different froms in the soil system by inoculation during the vegetative growth stages of the rice plant, when the nutritional requirement of the plants is at a maximum. Most of the N₂ fixed by cyanobacteria remained in the soil as the hydrolysable form (about 85%) during this period. Inoculation caused an insignificant increase in grain (8%) and straw (11%) yield, which was, however, accompanied by a significant increase in N uptake by the grain (30%) and an increase in total uptake of 15.3 kg N ha 1. Such beneficial effects of inoculation varied in magnitude with the application of organic sources, with farmyard manure (FYM) being the most effective. Application of urea N, on the other hand, markedly reduced such an effect.     

等养分条件下稻草还田替代双季早稻氮钾肥比例的研究

【目的】研究等量氮磷钾养分投入条件下,长期稻草原位全量还田配施化肥对双季早稻氮 (N)、磷 (P)、钾 (K) 养分吸收累积、转运及养分利用的影响,为南方稻区稻草资源有效利用,维持并提高土壤肥力及水稻合理施肥提供理论依据。【方法】以江西温圳国家级耕地质量监测点长期定位试验为研究对象,陆两优 996 为供试材料,在不施化肥和施用化肥基础上设稻草还田、稻草烧灰还田和稻草不还田共 6 个处理,除空白对照外,所有处理养分投入量相等。于 2015 年在早稻移栽期、分蘖期、幼穗分化期、抽穗期和成熟期取地上部植株样,分析水稻植株不同部位的 N、P、K 含量和累积量以及转运比例,并计算 N、P、K 养分利用效率。【结果】稻草还田提高了水稻产量,施肥条件下稻草还田处理比稻草烧灰还田和稻草不还田处理平均增产 2.9%～ 6.4%,比不施肥区产量增幅高达 23.8%～ 26.0%,且差异达显著水平。无论是施肥区处理,还是无肥区处理,与稻草烧灰还田和稻草不还田处理相比,稻草还田植株中 K 含量及 N、P、K 积累量在整个生育期均较高,而 N、P 含量在生育后期较高,N、P、K 积累量以施肥区处理大于相应的无肥区处理;稻草还田提高水稻 N、P、K 养分农学效率、回收率和养分偏生产力,且 N、K 差异达显著水平,同时显著增加 K 的收获指数;稻草还田还提高了抽穗至成熟期茎鞘中 N、P、K 的转运量、转运率及转移养分对籽粒的贡献率,而叶片各处理间差异不显著。【结论】稻草还田配施化肥能提高水稻产量,同时还可以调节 N、P、K 养分的积累和转运,提高养分的吸收利用效率。本试验条件下,稻草还田可替代化肥氮肥 (N) 29.5%、磷肥 (P₂O₅) 4.0% 和钾肥 (K₂O) 50.0%。综合考虑,稻草还田相比稻草烧灰还田而言是南方稻区土壤养分管理实现高效利用的有效途径之一。     

Effect of Conditioning Zinc Sulfate Heptahydrate (ZnSHH) with Zinc Oxide (ZnO) and Neem Oil on Growth,Productivity, Zinc Biofortification of Grain and Zinc Uptake by Basmati Rice

Commercial grade zinc (Zn) sulfate hepta hydrate (ZnSHH) is the most widely used source of Zn in India and several other countries for amelioration of Zn deficiency in crops. However, it releases water of hydration at temperature above 30°C and forms lumps on storage, which make it difficult to handle it and apply in fields. Therefore, conditioning of ZnSHH with ZnO and neem oil reduces the release of water of hydration and prevents lumps formation and can be well stored. Field experiments were conducted at the research farm of the Indian Agricultural Research Institute, New Delhi, India during rice growing seasons (July-November) of 2009 and 2010 to study the effect of conditioning ZnSHH with ZnO and neem oil on growth, productivity and Zn fortification of rice (Oryza sativa) grain and uptake by Basmati rice ‘Pusa 1121’. The experiment was conducted in a randomized block design with 3 replications comprised of 9 treatments of Zn fertilization. The present study shows that when conditioned with 2% ZnO and 4% neem oil ZnSHH improved yield attributes, grain and straw yields, Zn uptake and partial factor productivity (PFP), agronomic efficiency (AE), recovery efficiency (RE), and physiological efficiency (PE) of Zn in Basmati rice ‘Pusa 1121’. In general, ZnO was inferior to ZnSHH. Application of ZnSHH conditioned with 2% ZnO and 4% neem oil can be a better source of Zn for transplanted puddled Basmati rice on Zn deficient soils.     

Grain yield,growth response,and water use efficiency of direct wet-seeded rice as affected by nitrogen rates under alternate wetting and drying irrigation system

ABSTRACT

The present investigation aimed to determine the effectiveness of different nitrogen (N) rates on grain yield, growth, and water use efficiencies of direct wet-seeded rice and to create a relationship of N rates with growth parameters and dry matter production at different stages. The experiments compared six rates of nitrogen (0, 40,80,120,160, and 200 kg ha^–1N) replicated thrice in randomized complete block design in two conjunctive years of 2009–2010 and 2010–2011 at Bangladesh Rice Research Institute farm, Gazipur.The highest grain yield of 7.85 and 7.22 t ha^?1 was observed in N₂₀₀ treatment in 2009–2010 and 2010–2011, respectively. The relationship (R²) of total dry matter with leaf area index , leaf area duration, and crop growth rate indicated strong association during booting stage to achieved maximum dry matter during harvest. Water use efficiency varied 87–91% in different N levels.     

氮肥运筹模式对双季稻北缘水稻氮素吸收利用及产量的影响

在双季稻北缘地区,以常规品种早籼65和杂交组合香两优68为试验材料,在施氮量150.kg/hm2的条件下,研究了不同氮肥运筹模式对早稻产量及氮素吸收利用特性的影响。结果表明:减少基、蘖肥,提高穗肥比例可增加抽穗成熟期的叶片含氮量,使SPAD值维持较高水平,提高齐穗后的绿叶面积和有效叶面积率,提高群体光合势,有利于促进干物质积累而提高产量和氮素吸收,常规稻和杂交稻均以基∶蘖∶穗=50∶25∶25运筹模式产量最高;前氮后移增施穗肥因能为水稻整个生育期提供比较平衡的氮素供应,可促进氮素的吸收;氮肥当季利用效率随穗肥比例提高而增加,但氮肥的农学利用率与产量有更好的对应关系。基∶蘖∶穗=50∶25∶25的运筹模式是双季稻北缘地区早稻合理的施肥技术。     

Status of paddy soils as affected by paddy rice and upland soybean rotation in northeast Japan,with special reference to nitrogen fertility

To investigate the current available nitrogen (N) and chemical properties of paddy soils affected by crop rotation between irrigated paddy rice (Oryza sativa L.) and upland soybean [Glycine max (L.) Merr.] (paddy-upland rotation), topsoils were collected from 22 fields of four different farmers in the northeastern region of Japan. Regardless of organic material application, a significant negative correlation was found between available soil N and an increase in the proportions of upland seasons to total crop seasons after the initiation of paddy-upland rotation. Soil total N and total carbon (C) also tended to decrease with an increase in upland frequency. In fields with repeated applications of cattle manure compost, the soil available N was higher than in fields where only crop residue was applied. A significant negative correlation was also found between the soil available N:total N ratio and upland frequency. This indicates that the part of soil N related to available N was notably lost by the use of paddy fields as upland fields. In order to sustain available soil N over the minimum suitable level of 80?mg?kg^?1, upland frequency should not exceed 65% when only crop residues and no other organic materials are applied. The upland frequency can be raised by the repeated application of organic materials which maintain a higher level of available soil N. The results imply that care should be taken to maintain the N fertility of paddy soil at a suitable level in paddy-upland rotation, and that upland frequency and organic materials applied are important factors to do this.     

植物篱埂垄向区田技术对坡耕地水土和氮磷流失控制研究

针对松干流域农田面源污染控制需求,该文开展了植物篱埂垄向区田技术在坡耕地上的水土及氮磷流失控制效应研究。田间设置8个试验处理,包括两个对照即传统顺垄种植(CK1)与横垄种植(CK2)、3个间距的顺垄种植植物篱埂(1 m间距,T1;3 m间距,T2;5 m间距,T3)和3个间距的土埂(1 m间距,T4;3 m间距,T5;5 m间距,T6)。选择三叶草为植物篱材料。结果表明:1)与传统顺垄种植相比,横垄种植泥沙量减少46.9%,径流量减少52.9%;植物篱埂T1、T2与T3泥沙量分别减少44.6%、44.1%和42.1%,径流量分别减少50.6%、49.8%和49.2%;T4、T5和T6也能降低水土流失量,但与T1、T2与T3相比,泥沙流失量分别增加16.3%、12.6%和29.5%,径流量分别增加29.6%、46.8和76.9%;植物篱埂垄向区田技术的泥沙量与径流量控制效果相对接近横垄种植。2)与传统顺垄种植相比,各处理泥沙与径流TN浓度增大,TP浓度无变化;各处理的径流TN与TP浓度增大,其中各处理间的TN浓度有较大差异,TP浓度无明显差异;径流液TN浓度增加并没有引起农田氮流失增加,农田氮流失平均降低19.7%。3)考虑到经济投入问题,推荐植物篱埂间距3～5 m,较大坡度和坡上坡中采用较小间距,较小坡度和坡底采用较大间距;植物篱埂垄向区田技术能够提高玉米产量,平均增产5%～5.6%。