A synopsis of symbiotic nitrogen fixation and other apparently similar host‐microorganism interactions

Physical, physiological and biochemical processes occurring during colonization, infection, and nodule development and maintenance phases of an effective legume‐rhizobia symtiotic nitrogen fixation system are discussed. Limited knowledge of host reactions to invasion by rhizobia which result in failure to establish a symbiotic nitrogen fixation system are related to more thoroughly researched reactions of resistant host plants to invastion by pathogenic organisms. The most common resistant host responses are an increase in the production of phenolic compounds and phenol oxidizing enzymes. Many of the pheolic compounds or their quinones produced by enzymatic oxidation inhibit the action cell wall degrading enzymes and phytohormones, are antibiotic toward pathogenic organisms, and are phytotoxic to host plant cells. It is postulated that similar host responses result with rhizobia invasion and that the magnitude of these responses determine specificity of legume‐rhizobia symbiosis.     

Response of nitrogen fixation,nodule activities,and growth to potassium supply in water‐stressed broad bean

Three‐week‐old nodulated faba bean plants were subjected to two levels of water stress (0.5 and 0.25 field capacity; soil water content of 20 and 10%) for five weeks. Half of the stressed plants was treated with potassium chloride (KC1) at 10 (K₁) and 150 mg (K₂)/kg soil at the beginning of water deficit. Nodulation was examined and some nodule activities were assayed. Nodulation, nitrogenase activity, total nitrogen (N), and dry matter yield were significantly decreased by increasing stress but were significantly higher with the two levels of potassium (K) supply. Leghaemoglobin and protein contents of cytosol as well as nodule protease and invertase were severely depressed by drought stress. Soluble carbohydrate contents of nodules, however, was significantly increased. Protein and leghaemoglobin contents and enzyme activities were greater with K fertilization but less soluble carbohydrate was accumulated. The results indicate that K supply, particularly at the 150 mg/kg soil level, increased faba bean resistance to water stress.     

The use of the 15N‐dilution technique for field measurement of symbiotic nitrogen fixation

Abstract

Quantitative distribution of ¹⁵N in artificially and naturally enriched field‐growing legume‐based pastures are presented and compared. The results are discussed in terms of the assumption of the ¹⁵N‐dilution technique as a means of measuring symbiotic nitrogen fixation under field conditions.     

Influence of Azospirillum inoculation and nitrogen supply on grain yield,and carbon‐ and nitrogen‐assimilating enzymes in maize

Nitrogen (N) supply increased yield, leaf % N at 10 days after silking (DAS) and at harvesting, the contents of ribulose‐1,5‐bisphosphate carboxylase (RUBISCO) and soluble protein, and the activities of phosphoenolpyruvate carboxylase (PEPC), and ferredoxin‐glutamate synthase (Fd‐GOGAT), but not of glutamine synthetase (GS) for six tropical maize (Zea mays L) cultivars. Compared to plants fertilized with 10 kg N/ha, plants inoculated with a mixture of Azospirillum sp. (strains Sp 82, Sp 242, and Sp Eng‐501) had increased grain % protein, and leaf % N at 10 DAS and at harvest, but not grain yield. Compared to plants fertilized with either 60 or 180 kg N/ha, Azospirillum‐inoculated plants yielded significantly less, and except for GS activity, which was not influenced by N supply, had lower values for leaf % N at 10 DAS and at harvest, for contents of soluble protein and RUBISCO, and for the activities of PEPC and Fd‐GOGAT. Yield was positively correlated to leaf % N both at 10 DAS and at harvest, to the contents of soluble protein and RUBISCO, and to the activities of PEPC and Fd‐GOGAT, but not of GS, when RUBISCO contents and enzyme activities were calculated per g fresh weight/min. However, when enzyme contents and enzyme activities were expressed per mg soluble protein/min, yield was correlated positively to RUBISCO and PEPC, but negatively to GS. These results give support to the hypothesis that RUBISCO, Fd‐GOGAT, and PEPC may be used as biochemical markers for the development of genotypes with enhanced photosynthetic capacity and yield potential.     

Improvement of the nitrogen uptake induced by titanium (iv) leaf supply in nitrogen‐stressed pepper seedlings

The beneficial effect of titanium (Ti) on plant metabolism can result in more profitable use of fertilizer applied to a crop. A crop chamber experiment with paprika pepper (Capsicum annuum L., cv. Bunejo) seedlings under differential nitrogen (N) concentration levels in a nutrient solution (100% N, 75% N, 50% N, and 25% N) was performed. A third of the seedlings growing under each N support level remained Ti‐untreated and were used as the reference. Another third of the seedling received one and two 0.042 mM Ti(TV) ascorbate, pH 6.0, leaf spray treatments, respectively. The biomass production of the Ti‐untreated plants was only affected by the N supply of 50% or less. The Ti(IV) leaf spray treatments produced a biomass production greater than that of the corresponding reference plants, and both the 100% N+Ti and 75% N+Ti treatments had the highest biomass production. Seedlings receiving 50% N+Ti had a level of biomass production similar to that for the 100% N without Ti reference plants. In the same way, the 25% N+Ti treatment resulted in a plant fresh weight greater than that for the Ti‐untreated reference plants, although their biomass yields were not significantly lower than that for the corresponding 100% N and 75% N Ti‐untreated reference plants. Only the 50% N and 25% N Ti‐untreated plants had definite total‐N and nitrate‐nitrogen (NO₃‐N) unbalances as compared to the other N rate‐Ti treatments.     

Phosphorus‐deficiency effects on response of symbiotic N2 fixation and carbohydrate status in soybean to atmospheric CO2 enrichment

The impact of phosphorus (P) deficiency on response of symbiotic N₂ fixation and carbohydrate accumulation in soybean (Glycine max [L.] Merr.) to atmospheric CO₂ enrichment was examined. Plants inoculated with Bradyrhizobium japonicum MN 110 were grown in growth chambers with controlled atmospheres of 400 and 800 μL CO₂ L^‐1 and supplied either 1.0 mM‐P (P‐sufficient) or 0.05 mM‐P (P‐deficient) nitrogen (N)‐free nutrient solution. When plants were supplied with sufficient P, CO₂ enrichment significantly increased whole plant dry mass (83%), nodule mass (67%), total nitrogenase activity (58%), and N (35%) and P (47%) accumulation at 35 days after transplanting (DAT). Under sufficient P supply, CO₂ enrichment significantly increased starch concentrations in nodules compared to the normal atmospheric CO₂ treatment. Under normal CO₂ levels (400 μL L^‐1) nonstructural carbohydrate concentration (starch plus soluble sugar) was significantly higher in leaves of P‐deficient plants than in leaves of P‐sufficient plants in which nonstructural carbohydrate concentration exhibited a strong diurnal pattern. Under deficient P supply whole plant dry mass, symbiotic N₂‐fixation parameters, and N and P accumulation were not enhanced by atmospheric CO₂ enrichment. Phosphorus deficiency decreased nonstructural carbohydrate accumulation in nodules at the end of a 10‐day period in which functional activity was developing by 86% relative to P‐sufficient controls. While P deficiency elicited significant increases in the nonstructural carbohydrate concentration in leaves, it caused significant decreases in the nonstructural carbohydrate concentration in nodules over the diurnal cycle from 30 to 31 DAT. Collectively, these results indicate that the lack of a symbiotic N₂‐fixation response to atmospheric CO₂ enrichment by P‐deficient plants may be related to the decreased carbohydrate status of nodules.     

Comparison of understorey biological nitrogen fixation and biomass production in grassed‐down conventional and organic apple orchards in Canterbury,New Zealand

Abstract

In a previous study, the understorey biomass production and biological nitrogen (N) fixation of a grassed‐down organic apple orchard were presented. The aim of this paper is to report the results of a similar study of two conventional orchards in a nearby location and to compare the present results with those of the organic orchard. Biological N₂ fixation was determined in the field using the ¹⁵N isotopic dilution technique and the experiments were conducted over a two‐year period. Present results showed that substantial amounts of N (112 to 143 kg N ha^‐1.2 years^‐1) were fixed in the understorey of the conventional orchard and these were not significantly different from those of the organic orchard. However, the N₂ fixation was sustained in the conventional, but not in the organic orchards in the second year, probably due to regular additions of fertilizers in the conventional orchards. In both orchards, N₂ fixation was better correlated with clover than total dry matter yield. Seasonal effects found were highest N₂ fixation and biomass production occurring during late spring and early summer and lowest during winter. Climatic factors were investigated in one of the conventional orchards and it was found that seasonal effects were related to a combination of temperature and moisture deficit effects.     

Combined effects of plant growth‐promoting rhizobacteria and genistein on nitrogen fixation in soybean at suboptimal root zone temperatures

Application of plant growth‐promoting rhizobacteria (PGPR) or the plant to bacteria signal molecule genistein has been shown to increase nodulation and nitrogen (N) fixation by soybean [Glycine max (L.) Merr.] over a range of root zone temperatures (RZTs) and, specifically, off‐sets at least some of the ill‐effects of low RZTs. Two sets of controlled‐environment experiments, one on a growth bench and the other in a greenhouse, were conducted to examine the combined ability of both PGPR and genistein to reduce the negative effects of low RZT on soybean nodulation and N fixation. Each of two the PGPR strains, Serratia proteamaculans 1–102 and Serratia liquefaciens 2–68 were co‐inoculated with Bradyrhizobium japonicum USDA 110 preincubated with 17.5 (somewhat inhibitory), and 15°C (very inhibitory). At RZTs of 25 and 17.5°C PGPR strains and genistein in combination increased the number of nodules and the amount of Nn fixed. The most stimulatory effect was observed at 17.5°C for the combination: S. proteamaculans 1–102 plus B. japonicum USDA 110 pre‐incubated in 15 μM genistein under greenhouse conditions. For most treatment combinations the stimulatory effects of PGPR and genistein were additive at RZTs of 17.5 and 25°C. Surprisingly, the combination of these two factors resulted in antagonism at the very inhibitory RZT of 15°C. The results suggest that the negative effects of certain low RZTs could be more effectively off‐set by combined treatments of PGPR plus geneistin pre‐incubation of rhizobial cultures than by their individual treatment.     

Growth and nitrogen (N2) fixation response of arrowleaf clover to mineral nitrogen and 2(N‐morpholino)‐ethanesulfonic acid at low pH

Evaluation of legume response to acidic conditions can be difficult when using nutrient solutions because of fluctuations in solution pH. The organic buffer 2(N‐morpholino)‐ethanesulfonic acid (MES) has been used for stabilizing pH in nutrient solution studies. We evaluated the effectiveness of MES (5.0 mM) to stabilize solution culture at pH 5.5 with and without mineral N (0 or 1.0 mM NH₄NO₃) and its influence on growth and N₂ fixation of arrowleaf clover (Trifolium vesiculosum Savi). The buffer maintained pH stability ± 0.1 pH units in the presence or absence of mineral N. In the absence of mineral N, the quantity of N₂ fixed by plants grown with MES was not significantly different from that fixed by plants grown without MES. However, with mineral N, N₂ fixation was reduced 37% with addition of MES. Tissue analysis indicated a small increase in Ca and Mg concentration for plants grown with MES. Caution should be exercised in the use of MES in studies of N₂‐fixing legumes when mineral N is included.     

Microsite assessment of forest soil nitrogen,phosphorus, and potassium supply rates in‐field using ion exchange membranes

Abstract

A new method for microsite assessment of soil nutrient supply in forest soil was developed. The method involves the use of ion exchange membranes to assess differences in soil nitrogen (N), phosphorus (P), and potassium (K) supply rates in‐field over small depth increments in the forest floor (i.e., the L, F, and H horizons). Ion exchange membranes were buried and retrieved from the forest floor in an aspen forest stand in Saskatchewan, Canada. Small (6 mm diameter) sections of the membrane were cut out and ion concentration on the sections measured to provide a nutrient supply rate at that location. Soil nutrient supply rates at the site ranged from 4.6–6.0, 7.3–8.5, 11.6–21.5, and 122–196μg 10 cm²#lb2 h^‐1 for NH₄ ⁺‐N, NC₃ ^‐‐N, P, and K, respectively. On average, the highly humified H horizon had the highest N and P supply rates, followed by the F horizon, with the surface litter (L horizon) having the lowest N supply rates. The simplicity and sensitivity of the procedure make this method appropriate for in‐field assessment of differences in soil nutrient supply over small vertical and horizontal distance and was especially appropriate for the forest floor horizons in forest soils.     

Relationships between nitrogen rate,plant nitrogen concentration,yield and residual soil nitrate‐nitrogen in silage corn

Abstract

Nitrogen (N) fertilizer is a key factor of yield increase but also an environmental pollution hazard. The sustainable agriculture system should have an acceptable level of productivity and profitability and an adequate environmental protection. The objectives of this study were to determine the relationships between N rate, DM yield, plant N concentration (NC) and residual soil nitrate‐nitrogen in order to improve the predicted N rate in corn (Zea mays L.) silage. The experiment was conducted over a period of three years in the province of Quebec on three soil series in a continuous corn crop sequence. Treatments consisted of six rates of N: O, 40, 80, 120, 160, and 200 kg N ha^‐1 as ammonium nitrate applied at planting: broadcast and side banded. Four optimum N rates were calculated using different models: (i) economic rate base on fertilizer and corn price using the quadratic model (E); (ii) economic rate based on fertilizer and corn price using the quadratic‐plus‐plateau model (QP); (iii) critical rate based on linear‐plus‐plateau model (P); (iv) lower than maximum rate (L) corresponding to 95% of maximum yield. The optimum plant NC at all growing stages and the N uptake at harvest were calculated depending on these N rates and yields.

The NC of whole plant at 8‐leaf stage (25–30 cm plant height) of ear leaf at tasselling and of whole plant at harvest stage, the N rate, the N uptake at harvest and the DM yield were all significantly intercorrelated and affected by soils and years, but not affected by N fertilizer application method. The DM yield was linearly and significantly related to NC of whole plant at 8‐leaf stage (rv = 0.932**). At this stage, the average NC corresponding to the optimum N rate and yield was of 3.71, 3.68, and 3.66% as calculated with E, L, and P model, respectively. Our data suggest that the NC of whole plant at 8‐leaf stage may be used to evaluate the N nutrition status of plant and the required optimum N fertilizer rate. The NC of ear leaf at tassel stage was also significantly correlated to corn yield (r = 0.994**). It may be used as an indicator to evaluate the near‐optimum N rate in the subsequent years.

The N uptake by whole above‐ground plant at harvest was quadratically related to corn yield. Data show that at high fertilizer N rate, the N uptake still increased without significantly increasing yield. The N uptake was of 176.5, 163.0, and 155.0 kg N ha^‐1 using the E, L and P rates of 146, 126, and 115 kg N applied ha^‐1, respectively. The optimum N rate and yield were affected by soil type and year, but not by the method of N fertilizer application. The yield increased rapidly up to a N rate of about 120 kg N ha^‐1 and then quite slightly to a maximum N rate of 192 kg N ha^‐1. The optimum N rate was of 115 and 126 kg N ha^‐1 using the P and L model respectively and as high as 146.8 kg N ha^‐1 using the E model. The L model, using a much smaller N rate, gave a reasonably high yield compared to E rate (12.2 and 12.5 Mg ha^‐1, respectively). The data show that a relatively much lower N rate than maximum did not proportionally diminish the yield. Thus, for a difference of 40.4% between maximum N rate and P rate a difference of only 7.4% in yield was observed. Using the L model the differences in rate and yield were of 34.4% and 4.7%, respectively. The QP model gave no significant difference compared to E model.

At harvest the residual soil NO₃‐N increased significantly with increasing N fertilizer rate in whole of the 100 cm soil profile, but mainly in the top 40 cm soil layer. The total NO₃‐N found in 0–100 cm profile at rate of 0, 120 and 200 kg applied N ha^‐1 at planting was as high as 33.7, 60.5, and 74.5 kg N ha^‐1 respectively in a light soil and 37.5, 97.5, and 145.5 kg N ha^‐1 in a heavy clay soil. The difference in NO₃‐N content in the 60–100 cm layer between different applied N rate suggests that at harvest, part of fertilizer N applied at planting was already leached below the 100 cm soil layer. Results, thus, show that reasonably high corn yields can be obtained using more adequate N fertilizer rates which avoid the overfertilization and are likely to reduce the air and ground water pollution.     

Will selenium fertilization improve biological nitrogen fixation in lentils?

Lentil is a cool season food legume rich in protein and micronutrients. The objective of this study was to determine the effect of a low dosage of selenium (Se) on biological nitrogen (N) fixation, seed Se, and grain yield in lentils. The experiment was carried out at the Carrington Research and Extension Center, North Dakota, USA in 2012 and 2013. Six lentil genotypes were treated with three Se treatments. Application of selenate significantly increased percent Nderived from air (%Ndfa; 44%) compared to selenite (38%) and control (37%). In addition, selenate significantly increased lentil seed Se (1129 µg kg^?1) compared to selenite (844 µg kg^?1) and the control (542 µg kg^?1). Both %Ndfa and grain yield increased with Se application. Selenate was the most effective form to increase %Ndfa. More research is required to determine the biochemical relationships between lentil yield and the Nfixation under Se deficient soils.     

Lichen mycobionts of mycorrihizal symbiosis with nitrogen‐fixation of showy partridge pea

Effective mycorrhizal colonization is characteristic for nodulated Cassia genera that are adaptive to subhumid areas throughout the world. Growth, regeneration, and nitrogen (N) fixation occurs within regions of extreme soil and climatic environments that preclude persistent survival of other Leguminosae. Objectives of this study were to determine effective mycobiont components and adjunctive soil fertility factors governing growth, nodulation, and symbiotic N fixation of the important forage species, Showy Partridge Pea [Cassia Chamaecrista fasciculate (L.) Michx.] The perennial foliose lichen, Parmelia incurva, ubiquitous within extreme harsh drought and temperature regions, was utilized for mycorrizal mycobionts. Largest above ground plant growth, nodulation, and nitrogen fixation resulted with mycorrhizal colonization within lichen amended soil that received no other soil fertility treatments. Responses attained with phosphorus (P) and calcium (Ca) plant nutrient soil additions, without mycorrhizal mycobiont additions, were approximately half or less of effective mycorrhizal colonized plants. In general, yield response of mycorrhizal plants was reduced with plant nutrient additions throughout this study. Nitrate reductase (NR) and nitrate‐nitrogen (NO₃‐N) levels were significantly higher within nodule cytosol of nonmycorrhizal plants. Ureidoglycolate enzyme transformers and nodule cytosol ureide components were significantly greater for mycorrhizal colonized plants. These included urease (URC), allantoinase (ALTH), allantoicase (ALTC), and total ureides. However, differences were not significant for cytosol contents of pyruvate, amine‐amide N, aspartate transaminase (AST), glutamate dehydrogenase (GDH), glutamine synthetase (GS), and glutamate oxoglutarate trasaminase (GOGAT). Representative histological microscopy of mycorrhizal colonized Showy Partridge Pea are presented. Effective mycobiont propagules associative with lichen associations are apparently opportune commensal species and only functional as site specific sycophants governed by variable environmental conditions with lichen dissipation.     

Determination of nitrogen by microdiffusion in mason jars: III. Nitrogen and nitrogen‐15 in Kjeldahl digests

Abstract

Diffusion methods for quantitative determination and isotope‐ratio analysis of inorganic N in soil extracts were modified for use with Kjeldahl digests. The digest was diluted to 25 mL with deionized water, and an aliquot (to 6 mL) was transferred in a shell vial (17 mm dia., 60 mm long) to a 473‐mL (1‐pint) wide‐mouth Mason jar containing 15 mL of 8 M NaOH. The NH₃ liberated by overturning the vial inside the sealed jar was collected for 48 h at room temperature (24 h with orbital shaking) in 3 mL of boric acid‐indicator solution in a Petri dish, or in an acidified glass‐fiber disk, suspended from the Mason‐jar lid. Determinations of N and ¹⁵N by diffusion were in close agreement with analyses using conventional steam‐distillation and concentration techniques.     

Influence of nitrate‐nitrogen and ammonium‐nitrogen on calcium (45Ca2+) absorption in sorghum root tips

Abstract

Root‐tip, 1‐cm of Sorghum bicolor (L.) Moench cv SC283, SC574, GP‐10, and Funk G522DR were exposed to calcium (⁴⁵Ca²⁺) at pH 5.5 for 2‐hr in the presence of nitrate‐nitrogen (NO3^?‐N) or ammonium‐nitrogen (NH4+‐N). Nitrate (0.1 mM) induced significantly increased ⁴⁵Ca uptake in Funk G522DR, SC283, and GP‐10 while 0.01 mM NO₃ ^?‐N induced significantly increased ⁴⁵Ca'uptake in SC574, but ⁴⁵Ca absorption was significantly decreased at 1 mM NO₃—N. In the presence of the NH4⁺ ion, ⁴⁵Ca uptake was increased up to 8X that of the NH₄ ⁺‐N untreated roots. When ammonium chloride (NH₄CI) was used, the Cl^? tended to induce an increased ⁴⁵Ca uptake. Cultivar variation was present.     

Partitioning of biomass in water‐ and nitrogen‐stressed cotton during pre‐bloom stage

The partitioning of biomass between aboveground parts and roots, and between vegetative and reproductive plant parts plays a major role in determining the ability of cotton (Gossypium hirsutum L.) to produce a crop in a given environment. We evaluated the single and combined effects of water and N supply on the partitioning of biomass in cotton plants exposed to two N supply levels, 0 and 12 mM of N, and two water regimes, well irrigated and water‐stressed at an early reproductive stage. The N treatments began when the third true leaf was visible, while water deficit treatments were imposed over the N treatments when the plants were transferred into controlled‐environment chambers at a leaf area near 0.05 m². Both water deficits and N deficits inhibited total biomass accumulation and its partitioning in cotton. Water deficit alone and N deficit alone inhibited the growth of leaves, petioles, and branches, but did not inhibit growth of the stem and enhanced the accumulation of biomass in squares. When water deficit was superimposed on N deficit, leaf growth was inhibited, although to a lesser extent than when it was the sole stress factor, and the accumulation of biomass in squares was also inhibited. Yet, the dry weight of squares in plants exposed to water and N deficits was greater than that of non‐stressed plants. Water and N deficits, either alone or in combination, did not inhibit the growth of the tap root. Growth of lateral roots was not inhibited either by water deficit alone or in combination with N deficit, but was enhanced when plants were exposed to N deficit alone. Exposure to water deficit alone or in combination with N deficit decreased the shoot:root ratio through the inhibition of shoot growth. Exposure to N deficit alone decreased the shoot:root ratio through the combination of shoot growth inhibition and root growth enhancement.     

Quantification of the carbon and nitrogen cycles in long‐term field experiments in Prague

The oldest still existing long‐term field experiments in Czech Republic were founded in 1955. In Prague Ruzyné, there are five of nine experiments founded by ?karda. Data of two of these experiments (Block III and Block B) were used to evaluate the carbon and nitrogen cycles in time period 1966–1997. These two experiments have a similar design. They differ in the crop rotation. Four variants of organic and mineral fertilisation, receiving similar doses of fertilisers, have been selected. The same was calculated for the same time period for a mini‐plot bare fallow field experiment founded in 1958 by Novák.

The results of these experiments conducted in one locality (the same soil and climatic conditions) show the effect of the cultivated crops on the carbon and nitrogen cycles (comparing bare fallow experiment with the cropped ones), the effect of organic and mineral fertilisation (among all experiments), and the effect of crop rotation (comparing Block III to Block B) on these cycles.     

Effect of B,Mn and Zn on nodulation and N2‐fixation in southernpeas

Abstract

Two greenhouse studies were conducted to evaluate the effect of B, Mn and Zn on nodulation and N₂‐fixation of southernpea (Vigna unguiculata (L.) Halp.) cultivars ‘Freezegreen’, ‘Mississippi Silver’ and ‘Pinkeye Purple Hull’. The cultivars were grown in plastic pots with a Norfolk sandy loam (fine, loamy siliceous thermic, Typic Paleudult) soil treated with B, Mn and Zn at rates of 0, 5, 10 and 20 kg/ha each at pH levels 5.5, 6.0 and 6.5. At pH 6.5 all micronutrient treatments significantly increased nodulation and N₂‐fixation over the control (no micronutrient applied). The effects of B, Mn and Zn on nodulation and N₂‐fixation depended on the cultivar and soil pH. For plants given the 5 kg/ha B and Mn treatments, ‘Mississippi Silver’ produced the highest number of nodules and ‘Pinkeye Purple Hull’ the least. At 20 kg/ha Zn, nodulation of ‘Freezegreen’ was highest and ‘Pinkeye Purple Hull’ the lowest. As a whole, maximum nodulation was at 5 kg/ha B and Mn and 20 kg/ha for Zn. Nitrogen fixation rates responded similarly except that the optimum rate for Zn was 10 kg/ha. Seed yield of plants peaked at 5 kg/ha for B and 10 kg/ha for Zn, indicating a possible relation of N₂‐fixation to seed yield.     

Time of availability influences mixed‐nitrogen‐induced increases in growth and yield of wheat 1

Previous studies have indicated that under hydroponic conditions, spring wheat (Triticum aestivum) plants produce higher grain yields, more tillers, and increased dry matter when continuously supplied with mixtures of NO₃ and NH₄ than when supplied with only NO₃. The objective of this study was to determine if mixed N needs to be available before or after flowering, or continuously, in order to elicit increases in growth and yield of wheat. During vegetative development, plants of the cultivar ‘Marshal’ were grown in one of two nutrient solutions containing either a 100/0 or 50/50 mixture of NO₃ to NH₄ and, after flowering, half the plants were switched to the other solution. At physiological maturity, plants were harvested, separated into leaves, stems, roots, and grain and the dry matter and N concentration of each part determined. Yield components and the number of productive tillers were also determined. Availability of mixed N at either growth stage increased grain yield over plants receiving continuous NO₃, but the increase was twice as large when the mixture was present during vegetative growth. When the N mixture was available only during vegetative growth the yield increase was similar to that obtained with continuous mixed N. The yield increases obtained with mixed N were the result of enhanced tillering and the production of more total biomass. Although plants receiving a mixed N treatment accumulated more total N than those grown solely with NO₃, the greatest increase occurred when mixed N was available during vegetative growth. Because availability of mixed N after flowering increased the N concentration over all NO₃ and pre‐flowering mixed N plants, it appears that the additional N accumulation from mixed N needs to be coupled with tiller development in order to enhance grain yields. These results confirm that mixed N nutrition increases yield of wheat and indicate that the most critical growth stage to supply the N mixture to the plant is during vegetative growth.     

Repeated use of green-manure catch crops in organic cereal production – grain yields and nitrogen supply

Abstract

By restricted access to manure, nitrogen (N) supply in organic agriculture relies on biological N-fixation. This study compares grain yields after one full-season green manure (FSGM) to yields with repeated use of a green-manure catch crop. At two sites in south-eastern Norway, in a simple 4-year rotation (oats/wheat/oats/wheat), the repeated use of ryegrass, clover, or a mixture of ryegrass and clover as catch crops was compared with an FSGM established as a catch crop in year 1. The FSGM treatments had no subsequent catch crops. In year 5, the final residual effects were measured in barley.

The yield levels were about equal for grains with no catch crop and a ryegrass catch crop. On average, the green-manure catch crops increased subsequent cereal yields close to 30%. The FSGM increased subsequent cereal yields significantly in two years, but across the rotation the yields were comparable to those of the treatments without green-manure catch crop. To achieve acceptable yields under Norwegian conditions, more than 25% of the land should be used for full-season green manure, or this method combined with green-manure catch crops. The accumulated amount of N in aboveground biomass in late autumn did not compensate for the N removed by cereal yields. To account for the deficiency, the roots of the green-manure catch crops would have to contain about 60% of the total N (tot-N) required to balance the cereal yields. Such high average values for root N are likely not realistic to achieve. However, measurement of biomass in late autumn may not reflect all N made available to concurrent or subsequent main crops.