Short-term responses of soil nutrients and corn yield to tillage and organic amendment

Field experiments were conducted in 2010 and 2011 at the Agricultural College of Shiraz University to evaluate the effects of cattle manure and nitrogen (N) fertilizers on soil properties such as soil organic carbon (SOC), soil organic nitrogen (SON), soil electrical conductivity, soil pH and corn yield under two tillage systems. Treatments included tillage systems in two levels as conventional tillage and reduced tillage as subplots, cattle manure (0, 25 and 50 tons ha^?1) and N fertilizer (0, 125 and 250 kg N ha^?1) as sub-subplots. Results showed that SOC and SON were significantly affected by tillage system in both years of the experiment. SOC and SON were higher in reduced tillage compared to conventional tillage. Tillage system had no significant effect on grain yield, plant height and 1000 seed weight. Increased cattle manure rates at 25 and 50 tons ha^?1 increased grain yield by 27% and 38%, respectively, in 2010 and 25% and 25% in 2011. The results showed that application of cattle manure combined with N fertilizer might be an efficient management to increase soil productivity in southern Iran, in soils with poor organic content. Additionally, reduced tillage showed to be an efficient method to increase soil organic matter.     

基于SPAD-502与GreenSeeker的冬小麦氮营养诊断研究

用GreenSeeker和SPAD-502测定了不同氮素处理的冬小麦冠层NDVI与叶片SPAD值, 分析了它们与叶片全氮、叶绿素含量及产量间的关系。结果表明: 冬小麦抽穗期SPAD值和NDVI值均与叶绿素含量呈极显著正相关; 除抽穗期和返青期外, SPAD值与叶氮含量、叶绿素含量的相关系数在其余各生育期均达到显著或极显著水平; NDVI值与叶氮含量、叶绿素含量在拔节期、乳熟期的相关性同SPAD值一致; SPAD值可以进行叶绿素的诊断, NDVI值可以进行氮的诊断。氮营养诊断时期应该选择拔节期。通过回归建立了基于SPAD值、NDVI值的产量估测模型, 可以通过SPAD值、NDVI值对冬小麦产量进行估算。     

耕作方式和氮肥水平对旱地冬小麦籽粒品质的影响

为研究不同耕作方式和氮肥水平对旱地冬小麦籽粒品质的影响,选择常规耕作(CT)、秸秆还田(CTI)、全膜覆土穴播(PM)、免耕秸秆覆盖(NTS)4种耕作方式和75(N1)、150(N2)、225(N3)、300 kg·hm~(-2)(N4)4个氮肥水平,在典型的西北旱作雨养农业区甘肃省农业科学院小麦研究所清水试验站设计大田试验。结果表明,耕作方式和氮肥水平对小麦蛋白质及其组分含量、湿面筋、面团稳定时间、籽粒产量均有显著或极显著影响。与CT相比,PM显著提高了谷蛋白含量和谷醇比,延长了面团稳定时间,有利于加工品质的改善;而NTS的蛋白质总量、湿面筋、球蛋白、麦谷蛋白、谷醇比均显著低于CT。施氮显著提高了籽粒蛋白质、湿面筋、蛋白质各组分含量,增加了面团延伸性和吸水率。与N1相比,N4冬小麦的醇溶蛋白、麦谷蛋白、球蛋白、清蛋白分别增加了56.38%、27.60%、20.87%、17.03%;在4种蛋白组分中,施氮更有利于醇溶蛋白的累积,因此过量的氮肥应用显著降低了谷醇比、缩短了面团稳定时间和最大拉伸比值。同一氮肥水平下,不同耕作方式处理的小麦籽粒平均产量依次为PMCTICTNTS,PM下的平均产量为6 259.63 kg·hm~(-2),比CTI、CT、NTS分别高6.24%、10.54%和20.95%。同一耕作方式下,不同氮肥水平处理的小麦籽粒平均产量依次为N2N3N4N1,其中N2下小麦籽粒平均产量达到6 184.64 kg·hm~(-2),比N3、N4、N1分别高5.78%、7.31%、19.06%。综上可知,在甘肃半干旱雨养农业区实施全膜覆土穴播栽培模式,并增施150 kg·hm~(-2)氮肥,既能大幅度增加小麦籽粒产量,也有利于加工品质的改善,是该地区小麦生产实现高产和优产的最佳耕作和供氮模式。本研究结果为甘肃旱作雨养农业区冬小麦的高产、优质栽培提供了理论参考。     

施氮对不同肥力土壤小麦氮营养和产量的影响

【目的】农田养分供应是由土壤基础肥力和肥料投入共同决定的,不同土壤肥力下土壤养分供应能力和特征也不同。本文研究了河南省高、低肥力田块下,不同施氮量对小麦主要生育时期植株氮素营养和土壤硝态氮及产量的影响,以期为河南省同类生产条件下氮肥的合理施用和产量的提升提供参考和依据。【方法】2015—2016年,以小麦品种矮抗58为供试材料进行大田试验,分别设置0、120、225、330 kg/hm^2 4个施氮处理(表示为N0、N1、N2、N3),在开花期到成熟期调查施氮量对土壤硝态氮及产量的影响;在开花期、花后10天和花后20天,测定施氮量对小麦旗叶到倒4叶的叶片氮含量、SPAD值和氮素积累量,以及对植株和所有叶片氮含量的影响。【结果】从开花期到成熟期土壤中硝态氮含量随着施氮量的增加而增加,高肥力田块的土壤硝态氮含量显著高于低肥力田块的土壤硝态氮含量。施氮能显著增加低肥力田块产量,但是高肥力田块的产量均高于低肥力田块,与不施氮相比,低肥力田块的产量最大增幅是高肥力田块产量最大增幅的2.63倍。N1和N2处理下,在开花期和花后10天倒2叶的SPAD值高肥力田块显著高于低肥力田块,但在花后20天低肥力田块显著高于高肥力田块。在N1、N2和N3处理下,旗叶的氮含量在花后10天高肥力田块显著高于低肥力田块,但在花后20天则显著相反。开花期到花后20天,对于低肥力田块旗叶的氮素积累量对上4叶的贡献率最大(N0除外),最高达52.6%;高肥力田块,旗叶和倒2叶对上4叶的氮素积累量贡献率处在同等重要的位置,最高分别达39.9%和39.7%。花后10天到花后20天,高肥力田块不同叶位的氮素转运量和转运率均高于低肥力田块(N0除外)。【结论】增施氮肥可以通过提高土壤硝态氮含量来提高土壤供氮能力,高肥力田块的叶片转运量和转运率比低肥力田块高,低肥力田块通过提高施氮量增加的产量低于高肥力田块下的产量,因此,需改善农田基础肥力来提高产量。通过对高、低肥力条件下产量的分析发现,达到最高产量时的施氮量分别为213kg/hm^2和287 kg/hm^2。     

不同耕作方式下缓释肥对夏玉米产量及氮素利用效率的影响

【目的】氮肥中氮素的合理释放是提高作物产量及氮素利用效率的重要措施之一,耕作方式也可显著影响氮肥施用效果。本文比较了浅旋、 免耕和深松三种耕作方式下缓释肥和常规施肥对夏玉米干物质积累、 叶面积指数及植株氮素积累的影响,为玉米缓/控释肥的合理应用和耕作方式的选择提供科学依据和技术支撑。【方法】试验于2013年至2014年在河南新乡进行。采用裂区设计,以耕作方式为主区,设浅旋(R)、 免耕(N)和条带深松(S)3种耕作方式; 肥料类型为副区,设不施氮(CK)、 缓释肥(SRF)和常规施肥(CCF)3个处理。【结果】相同耕作方式下,缓释肥处理的夏玉米产量及氮肥利用效率显著高于常规施肥; 相同肥料处理,其条带深松耕作的夏玉米产量及氮肥利用效率显著高于浅旋和免耕直播处理。各处理中,以条带深松下的缓释肥处理产量及氮肥利用效率最高,显著高于其他处理,其中两年产量平均增幅为13.4%~59.2%,氮肥农学利用效率(AEN)增幅为27.9%~72.7%,氮素表观利用率(REN)增长15.1~55.7个百分点。分析表明,干物质积累和氮素积累,尤其是花后干物质和氮素积累的增加是产量提高的主要原因。 在N、 R和S 三种耕作方式下,缓释肥处理的花后干物质积累量较常规施肥分别提高13.0%、 12.7%和18.7%; 花后氮素积累量分别提高14.4%、 16.8%和17.8%,其中条带深松耕作的增幅显著高于浅旋和免耕直播。条带深松下缓释肥处理花后干物质和氮素积累量显著高于其他处理。【结论】与传统耕作方式和施肥方式相比,条带深松耕作和缓释肥的施用均显著提高了夏玉米的产量及氮素利用效率,各处理中以条带深松耕作下缓释肥处理的夏玉米产量及氮素效率最高,因此,条带深松深施缓释肥可作为黄淮海区一项有效的合理耕作与施肥措施。     

PERFORMANCE OF DUALEX IN SPRING WHEAT FOR CROP NITROGEN STATUS ASSESSMENT,YIELD PREDICTION AND ESTIMATION OF SOIL NITRATE CONTENT

Dualex and SPAD are devices developed for the purpose of testing crop nitrogen (N) status. These instruments were used in a wheat experiment in order to compare their respective performance in assessing leaf nitrogen (N) concentration, response to N topdressing application, soil nitrate (NO₃)-N levels and in predicting grain yield. The experiment included different N rates in 2005 and 2006 in the Montérégie region of Quebec, Canada. Dualex readings correlated negatively with SPAD readings, leaf N concentration, soil NO₃-N content and wheat grain yield. SPAD alone and the ratio of SPAD to Dualex measurements (Chl/Phen) were linearly related to N application rate but no effect of N application rate was found for individual Dualex parameters. However, both SPAD and Dualex readings were affected by year effects. The Dualex was also capable of indirect evaluation of in-season soil NO₃-N accumulation and the prediction of wheat yield, but more so as Chl/Phen.     

相对SPAD值用于不同品种夏玉米氮肥管理的研究

采用田间试验研究了不同氮肥处理、不同玉米品种及关键生育期间的SPAD值差异和基于相对SPAD阈值的氮肥管理对氮肥用量、子粒产量、氮肥利用率和土壤氮素变化的影响。结果表明,两品种玉米各关键生育期的SPAD值开始随施氮量的增加而显著增加,施氮量超过N 210 kg /hm2后不再显著增加;郑单958和冀农一号大喇叭口期的相对SPAD值与产量的关系符合线性加平台模型,其平台相对SPAD值分别为0.976和0.981;两玉米品种和不同生育期间的绝对SPAD值差异显著,利用相对SPAD值可消除品种和生育期间的SPAD值差异。玉米关键生育期追肥量和总施氮量均随预设相对SPAD阈值的增加而增加,基于相对SPAD阈值的氮肥管理能在保持高产的同时较农民习惯施肥显著降低氮肥用量、田间氮素表观损失和收获后土壤无机氮残留、提高氮肥利用率;本试验条件下,保持玉米高产高效的适宜相对SPAD阈值为0.95～0.98,此阈值管理下,郑单958和冀农一号的产量较农民习惯施肥没有降低,而氮肥用量降低了42%,氮肥回收利用率和农学效率分别增加了18.6、20.0个百分点和6.0、6.5 kg/kg。     

Tillage and Nitrogen Application Methodology Impacts on Corn Grain Yield

ABSTRACT

Increased fuels costs have prompted many producers to consider conservation tillage techniques and single pass applications of nitrogen (N) fertilizer and herbicide to reduce fuel expenses. The objective of this study was to determine the impacts of tillage and nitrogen application methodology on corn grain yield. The experiment was conducted from 2002–2005 at the Northwest Research Station of the Ohio Agricultural Research and Development Center (OARDC) near Hoytville, OH. Six different tillage regimes were established as main plots: no-till, fall disc-field cultivator, Aerway tool tillage, early planted strip-till, late planted strip-till, and zone deep-till. Subplots consisted of either a single-pass application of broadcast, surface applied urea-ammonoium nitrate (UAN) representing a weed ‘n’ feed application, a split application of nitrogen between planter applied and sidedress N (subsurface injected N), or an unfertilized control. The rate of N for the different application methodologies was 168 kg ha^?1. Dry conditions during the 2002 growing season resulted in very poor corn yield and thus little response to tillage or N application. In 2003, the split treatment maximized corn yield likely due to minimized ammonia volatilization independent of tillage regime. Surface broadcast applications of UAN resulted in lower grain yields in conservation tillage treatments compared to split nitrogen applications in 2004. No statistical differences were noted between the two application methods in the conventional tillage treatments. In 2005, no yield differences could be attributed to N application methodology across tillage treatments. From this study it was concluded that surface broadcast application of UAN can result in yield loss, especially in conservation tillage systems.     

主动遥感光谱仪Greenseeker与SPAD对玉米氮素营养诊断的研究

以手持式主动遥感光谱仪Greenseeker和叶绿素仪SPAD对玉米不同氮素水平下各个生育期的NDVI值及叶片SPAD值进行测试,研究不同氮素对玉米群体和个体营养状况的变化以及田间条件下简便、快速、非接触性的作物氮素营养状况诊断方法。结果表明,在一定的范围内随着氮肥用量的增加NDVI值也增加,氮肥施用量为N 300kg/hm2时NDVI值达到最高,NDVI 与氮肥施用量符合线性加平台的关系;玉米不同生育期间NDVI 值变化明显,苗期NDVI值比较低,大喇叭口期NDVI 值达到最高,以后逐渐下降并在抽雄期后趋于稳定。SPAD值与NDVI 值的变化趋势相一致,SPAD值与叶绿素含量成正相关关系,大喇叭口期完全展叶的SPAD 与产量存在正相关关系。手持式主动遥感光谱仪Greenseeker和叶绿素仪SPAD结合能够对玉米的氮素营养状况作营养诊断。     

不同施氮量下缺钾对水稻叶片营养及生理性状的影响

【目的】氮和钾是作物生长所必需的大量元素,在水稻生长发育、产量形成等过程中发挥着至关重要的作用。南方稻田缺钾以及氮钾肥不合理施用已成为限制水稻高产的重要影响因子。本研究在田间条件下,探讨了不同施氮量下缺钾对水稻生长发育与叶片生理特性的影响,进而阐明缺钾导致营养生长期水稻叶色暗绿的营养及生理机制。【方法】采用两因素完全随机设计田间试验,因素A为不同施氮水平,包括不施氮、低氮（N 90 kg/hm2）和正常施氮（N 180 kg/hm2）;因素B为不同施钾水平,包括不施钾和正常施钾（K₂O 120 kg/hm2）。测定水稻分蘖期和幼穗分化期地上部干物质,叶面积指数,叶片氮、钾、镁和叶绿素含量（叶色值）,叶片含水率、叶片可溶性糖含量、比叶重以及叶片SPAD值。【结果】1）在不施氮条件下缺钾对水稻分蘖期和幼穗分化期干物质、叶面积指数均无显著影响,而在施氮条件下显著降低水稻分蘖期和幼穗分化期干物质、叶面积指数;随施氮量的增加,缺钾对干物质及叶面积指数的影响加剧,其中N₁₈₀K₀处理的降幅最为明显;氮钾交互作用对水稻各生育期的干物质和叶面积指数均有显著或极显著影响。2）在不施氮条件下缺钾对分蘖期和幼穗分化期叶片氮含量和叶绿素含量、叶片可溶性糖含量、比叶重以及叶片SPAD值均无显著影响,而在施氮条件下以上各指标显著增加,其中N₉₀K₀处理的叶片氮含量和叶绿素含量均可以达到N₁₈₀K₁₂₀处理水平;无论施氮与否,缺钾均显著降低分蘖期和幼穗分化期叶片钾含量,而显著增加叶片镁含量。3）回归分析结果表明,比叶重与叶片可溶性糖含量呈极显著正相关关系（P < 0.01）。【结论】水稻干物质、叶面积指数、叶片营养及生理状况、叶色表现等对缺钾的响应明显受到施氮量的影响。在施氮条件下缺钾造成叶片中可溶性糖大量积累,进而导致比叶重增加;结合田间试验观察及叶片营养及生理性状可知,水稻叶色（叶绿素含量）在不施氮条件下不受缺钾的影响;而在施氮条件下,缺钾造成水稻叶片单位质量及单位叶面积氮含量和叶绿素含量显著增加,这是田间条件下水稻叶色呈现暗绿的主要原因,从而也影响生育期植株氮素营养诊断。     

追氮量对黑小麦品种(系)籽粒产量和品质的影响

为探究追氮量对不同黑小麦品种(系)籽粒产量和品质的影响,本研究采用大田裂区试验设计,主区为11个黑小麦品种(系),副区为追施氮量0(N0,对照)、34.5(N1)、51.8(N2)、69.0(N3)kg·hm²,于小麦灌浆期测定旗叶叶绿素含量(SPAD值),成熟期测定籽粒产量及产量构成要素、籽粒氮素含量和品质指标。结果表明,旗叶SPAD值在追氮处理下均增加,除B145-2、L10056、L10058的旗叶SPAD值在N1最高,其余8个品种(系)的旗叶SPAD值均随追氮量增加而升高。追施氮肥均有利于增加黑小麦产量,与N0相比,N1、N2和N3的产量增幅分别为15.2%~99.7%、17.5%~80.9%和0.7%~69.9%。11个品种(系)获得最高产量的适宜追氮量因品种而异,其中L10056、B120-2、运黑14207籽粒产量随追氮量增加而增加,B145-2、L10058籽粒产量在N2最高,其余6个品种(系)籽粒产量在N1最高。追氮量、品种及二者交互均对产量及构成因素存在极显著影响。追施氮肥能够提高多数黑小麦品种籽粒氮素含量,其中B145-2、L10058和运黑14207在N2最高,18鉴46、临黑131、冬黑1号、临黑187、2002-4、运黑161在N1最高。追氮量与籽粒产量、氮含量、蛋白含量呈正相关(相关性不显著),而产量与籽粒蛋白含量及多数品质指标存在极显著负相关关系。综合考虑黑小麦产量及品质的提高,追氮量在34.5~51.8 kg·hm²之间较为适宜。本研究结果可为黑小麦合理施用氮肥提供参考。     

追氮量对黑小麦品种(系)籽粒产量和品质的影响

为探究追氮量对不同黑小麦品种(系)籽粒产量和品质的影响,本研究采用大田裂区试验设计,主区为11个黑小麦品种(系),副区为追施氮量0(N0,对照)、34.5(N1)、51.8(N2)、69.0(N3)kg·hm²,于小麦灌浆期测定旗叶叶绿素含量(SPAD值),成熟期测定籽粒产量及产量构成要素、籽粒氮素含量和品质指标。结果表明,旗叶SPAD值在追氮处理下均增加,除B145-2、L10056、L10058的旗叶SPAD值在N1最高,其余8个品种(系)的旗叶SPAD值均随追氮量增加而升高。追施氮肥均有利于增加黑小麦产量,与N0相比,N1、N2和N3的产量增幅分别为15.2%~99.7%、17.5%~80.9%和0.7%~69.9%。11个品种(系)获得最高产量的适宜追氮量因品种而异,其中L10056、B120-2、运黑14207籽粒产量随追氮量增加而增加,B145-2、L10058籽粒产量在N2最高,其余6个品种(系)籽粒产量在N1最高。追氮量、品种及二者交互均对产量及构成因素存在极显著影响。追施氮肥能够提高多数黑小麦品种籽粒氮素含量,其中B145-2、L10058和运黑14207在N2最高,18鉴46、临黑131、冬黑1号、临黑187、2002-4、运黑161在N1最高。追氮量与籽粒产量、氮含量、蛋白含量呈正相关(相关性不显著),而产量与籽粒蛋白含量及多数品质指标存在极显著负相关关系。综合考虑黑小麦产量及品质的提高,追氮量在34.5~51.8 kg·hm²之间较为适宜。本研究结果可为黑小麦合理施用氮肥提供参考。     

玉米叶片SPAD值、全氮及硝态氮含量的品种间变异

研究比较两种土壤肥力条件下,4个春玉米品种在喇叭口期至成熟期间叶片SPAD值、全氮及硝态氮含量的变异程度、及其与氮素积累和产量形成的关系,以期为不同品种植株的氮素营养测试指标的优化提供依据。结果表明,叶片SPAD值与产量、吸氮量及生物量呈显著相关,该值主要受氮肥水平影响,并因土壤肥力而变异。从喇叭口期至灌浆期间平均变异幅度为17.7%,但品种间变异很小,平均仅为4.3%。说明利用SPAD值诊断玉米氮素营养时,其诊断指标不需要因品种而调整,但需要因不同肥力而调整。在新立城低肥力条件下,喇叭口期(V12)和抽雄期(VT)的SPAD临界值指标分别为46.1和57.8;在德惠高肥力条件下,两个时期的SPAD值临界值较为接近,分别为59.9和60.3。植株叶片硝态氮含量在土壤肥力间及品种间变异均较大,变异幅度分别为43.1%和29.3%,且与产量、吸氮量及生物量的相关性均较差,不适于在大面积范围内单独作为玉米氮素营养状况的评价指标。     

栽培方式对水稻氮素吸收利用与分配特性影响的研究

2006和2007年,在桶栽条件下,以早稻（三系籼型杂交稻金优253）为试材,应用15N示踪技术研究了稻草还田免耕、免耕、稻草还田常耕、常耕4种栽培方式对水稻氮素吸收利用与分配特性的影响。结果表明,1）水稻植株吸收的氮肥数量及其在体内的分配与土壤耕作方式密切相关; 免耕提高了水稻对肥料氮的吸收比率及在子粒和根中的分配比率,但降低了在叶和茎中的分配比率。2）免耕水稻产量和氮素吸收总量与常耕水稻差异不显著,但免耕水稻吸收的氮素中来源于肥料的比例比常耕水稻的大; 免耕水稻的氮肥回收效率高于常耕水稻,但氮素的稻谷生产效率差异不明显。3）稻草还田对氮肥的吸收利用有显著的影响。免耕条件下,稻草还田降低了基肥和分蘖肥氮素的积累量,增加了穗肥氮素积累量,氮肥总积累量呈下降趋势; 常耕条件下,稻草还田提高了各个时期的肥料氮素积累量,氮肥的总积累量增加显著。4）稻草还田提高了免耕水稻和常耕水稻产量,2007年其差异达显著水平。免耕条件下稻草还田降低了氮素的回收效率,但常耕条件下稻草还田提高了氮肥的回收效率。     

氮锌配施对不同玉米品种灌浆期生理特性和籽粒氮锌含量的影响

提高粮食作物中可食部分的锌生物有效性是解决人体缺锌的重要措施。为研究氮锌肥料施用对玉米籽粒锌营养的影响,本研究以郑单958和谷神玉66为试验材料,在大田条件下研究3个氮水平(90、180 和225 kg N·hm^-2)和2个喷锌处理(0和4.5 kg·hm^-2 ZnSO₄·7H₂O)下玉米籽粒产量和氮锌含量以及灌浆期叶片生理特性的变化。结果表明,吐丝后,与施氮量90 kg·hm^-2处理相比,施氮量180和225 kg·hm^-2处理提高了吐丝后穗位叶SPAD值及硝酸还原酶(NR)、谷氨酰胺合成酶(GS)、碳酸酐酶(CA)、超氧化物歧化酶(SOD)活性和灌浆后期PSⅡ综合性能指数(PI),降低了丙二醛(MDA)含量。施锌能提高吐丝后穗位叶CA、SOD、过氧化物酶(POD)、过氧化氢酶(CAT)活性和灌浆后期SPAD值和PI,降低MDA含量。2个品种相比,谷神玉66灌浆后期穗位叶SPAD值、叶片初始荧光(F_o)和最大荧光(F_m)较高,而灌浆前期穗位叶PI和吐丝后NR、CA、SOD以及POD活性则以郑单958较高。施氮量为90 kg·hm^-2时,玉米籽粒产量平均为8.55 t·hm^-2,随着施氮量增加,玉米籽粒产量显著提高。籽粒中氮含量以施氮量180 kg·hm^-2时最高,为14.85 g·kg^-1。施氮量90和180 kg·hm^-2时,籽粒锌含量平均为27.2 mg·kg^-1,显著高于施氮量225 kg·hm^-2处理。与不施锌相比,喷锌后玉米籽粒产量未有显著变化,籽粒中氮、锌含量分别增加了11.7%和18.0%。郑单958籽粒产量较谷神玉66提高了3.8%,籽粒氮锌含量则分别减少了11.9%和5.3%。综合来看,施氮量为180 kg N·hm^-2时,与喷施ZnSO₄·7H₂O 4.5 kg·hm^-2 配合施用能够增强玉米灌浆期叶片SPAD和荧光特性,提高氮锌代谢关键酶活性,增强氧自由基清除系统酶活性,减弱膜脂过氧化作用的伤害,促进籽粒中氮、锌的吸收和累积。本研究结果可为玉米生产中优化锌生物强化措施提供理论依据。     

烤烟氮素营养诊断及精准施肥模式研究

为解决烤烟生产中存在过高施氮或不合理的施肥技术措施致使烟叶产质量下降和氮素利用效率低下等突出问题,该研究于2008-2010年在广东韶关烟草产区以烤烟品种K326为材料,研究不同施氮水平下不同叶位及叶片不同位置上的叶绿素仪测定值(SPAD值)、叶绿素含量、总氮含量的变化特征及其与产质量的相互关系,分析实时氮肥管理(RTNM)模式下不同SPAD施肥阈值对烟草产质量及氮肥利用率的影响,结果表明:顶三叶的叶中位置SPAD值与叶片叶绿素含量、总氮含量的相关性最好,且变异系数小,是较为理想的指示叶或参照叶;获得较好产质量烤烟时的SPAD值在伸根期和旺长期的阈值范围为38.3～47.2。在设置不同SPAD阈值的RTNM模式下,SPAD阈值40.5~43范围内(氮肥用量为75～110kg/hm2)能获得较高的烟叶产量、产值,最高产量和产值分别为2844.09kg/hm2和26989.24元/hm2,烟叶化学成分较为协调。此外,通过与农民习惯施肥模式(167.8kg/hm2)相比较,在SPAD设定阈值为43的RTNM模式下,相应的氮肥用量为110kg/hm2,氮肥农学利用率和吸收利用率分别提高53.0%、46.5%,产值提高了7.4%,调制后烟叶的淀粉、总氮、烟碱含量显著下降,烟叶化学成分更为协调,内在质量较好。     

Leaf and canopy optical characteristics as crop‐N‐status indicators for field nitrogen management in corn

The primary constraint of predicting the economic optimum nitrogen rate (EONR) for corn (Zea mays L.) is the high variability of soil nitrogen (N) supply due to environments, soil types, manure, and cropping histories. Portable instruments have been developed to measure leaf and canopy optical characteristics for determining plant N status. The objectives of this field study were to: (1) evaluate leaf and canopy optical properties including transmittance, reflectance, and fluorescence as indicators of corn N status with soil types, developmental stages, and N‐application rates, (2) compare the efficiency of two commercial radiometers that are designed to measure canopy reflectance, and (3) assess the constraints of these crop‐based indicators as a possible guide for real‐time N sidedressing in corn. Field experiments with different levels of N, soil types, and corn hybrids were conducted at three sites in Ottawa, ON, Canada, in 2004 and 2005. Leaf chlorophyll concentrations (SPAD chlorophyll meter), chlorophyll fluorescence (OS‐30), leaf area, and canopy reflectance (NDVI measured by CropScan and GreenSeeker radiometers) were simultaneously measured at several growth stages, while grain yield was determined at harvest. Our results show that canopy reflectance (NDVI) displayed similar efficiency as an indicator of N status on both soil types and corn hybrids in the two consecutive years. The chlorophyll readings often differentiated N‐deficient from N‐sufficient plots and therefore were a promising indicator for predicting corn N requirements. The fluorometer device evaluated in this study was unable to characterize corn N status.     

Effect of Nitrogen and Trinexapac-Ethyl Rates on the SPAD Index of Wheat Leaves

ABSTRACT

The objective of this study was to evaluate the effect of nitrogen (N) and trinexapac-ethyl (TE) rates on the SPAD index in wheat flag leaf. The treatments were five N rates (30, 60, 90, 120, 150 kg ha^?1) combined with four TE rates (0, 63, 125, 188 g ha^?1). The experiment was carried out in a randomized block design with four repetitions. SPAD index, leaf N content and grain yield showed quadratic response to the increase in N rates, whereas area, wet and dry weight of flag leaf presented linear increase. TE caused linear increase in SPAD index, linear decrease in leaf area, reduction in grain yield with smaller N rates and increase with larger N rates. The N content, and the wet and dry weight of flag leaf were not affected by TE. SPAD calibration to estimate N status in wheat should be specific for each TE rate.     

氮肥运筹模式对双季稻北缘水稻氮素吸收利用及产量的影响

在双季稻北缘地区,以常规品种早籼65和杂交组合香两优68为试验材料,在施氮量150.kg/hm2的条件下,研究了不同氮肥运筹模式对早稻产量及氮素吸收利用特性的影响。结果表明:减少基、蘖肥,提高穗肥比例可增加抽穗成熟期的叶片含氮量,使SPAD值维持较高水平,提高齐穗后的绿叶面积和有效叶面积率,提高群体光合势,有利于促进干物质积累而提高产量和氮素吸收,常规稻和杂交稻均以基∶蘖∶穗=50∶25∶25运筹模式产量最高;前氮后移增施穗肥因能为水稻整个生育期提供比较平衡的氮素供应,可促进氮素的吸收;氮肥当季利用效率随穗肥比例提高而增加,但氮肥的农学利用率与产量有更好的对应关系。基∶蘖∶穗=50∶25∶25的运筹模式是双季稻北缘地区早稻合理的施肥技术。     

Leaf senescence induced by nitrogen deficiency as indicator of genotypic differences in nitrogen efficiency in tropical maize

Nitrogen efficiency is a complex trait. Identification of secondary plant traits correlating with N efficiency would facilitate the breeding for N‐efficient cultivars. Sixteen tropical maize cultivars differing in grain yield at low N supply (N efficiency) under field conditions in Zimbabwe exhibited a significant negative correlation between N efficiency and leaf senescence during grain filling. The same cultivars were studied for leaf senescence under N deficiency in a short‐term nutrient‐solution experiment. Leaf chlorophyll contents as estimated by SPAD values and photosynthesis rates were used as measures for leaf senescence. Cultivars differed both in SPAD values and photosynthesis rates of the older leaves during N deprivation. Significant negative correlations were found between SPAD values, photosynthesis rates in the nutrient‐solution experiment, and leaf‐senescence scores in the field experiments, and positive correlations were found between photosynthesis rates and grain yield under low‐N conditions in the field. Relationships between physiological root parameters, which were also investigated in the nutrient‐solution experiment, and N uptake or grain yield of the cultivars in the field could not be established. It is concluded, that the assessment of the capacity of a genotype to maintain a higher photosynthetic capacity of older leaves during N deficiency–induced senescence at the seedling stage is a suitable selection parameter for the N efficiency of tropical maize cultivars.