Existence of salt glands on leaves of kallar grass (Leptochloa fusca L. Kunth.)

Light microscopic studies have established the existence of salt glands on “Kallar grass”; leaf blades. The salt glands resembled the two‐cell type structure described for some halophytic genera within the Gramineae. The secreted salts crystallized and formed whiskers when the plants were grown under continuously high saline conditions. The salt secretion appears to be selective. The secretion of Na exceeded that of K and considerable quantities of Cl were also extruded, preferentially in concomitance with Na. The significance of growth hormones and Ca in regulating the salt secretion process is discussed.     

The relationship between vesicular-arbuscular mycorrhiza and lime: Associated effects on the growth and nutrition of brachiaria grass (Brachiaria decumbens)

Summary We studied the effects of limining on growth and nutrient concentrations of Brachiaria decumbens inoculated with five vesicular-arbuscular mycorrhizal (VAM) fungal assemblages which orginated from soils with different acidity. Liming increased plant growth when applied at rates up to 3 g kg^-1 soil and depressed growth at higher rates. Mycorrhizal plants grew better than non-mycorrhizal ones in unlimed soil and also liming rates of 4.5 and 6.0 g kg^-1 soil. The growth amelioration effects of VAM in highly acid or over-limed soils were related to nutrient uptake. VAM fungi isolated from an acidic soil exhibited a high symbiotic effectiveness and were better adapted to unlimed soil than those that originated from non-acidic soils. VAM root colonization, 90 days after planting, was little affected by liming. Fungal spore production and species compositions were highly affected by liming. A mixture of Glomus diaphanum and Glomus occultum predominated in unlimed soils inoculated with VAM assemblages isolated from non-acidic soils. In these fungal assemblages, an increased liming rate favored Glomus etunicatum over the other VAM fungi. Gigaspora margarita sporulated abundantly when introduced into unlimed soils, but rarely in limed soils. VAM appear to be crucial for the establishment of brachiaria pastures in the nutrient-deficient acidic soils of Central Brazil. It is suggested that liming may cause striking shifts in VAM populations which may, in turn, have a long-term impact on agricultural productivity in the tropics.     

K-priming positively modulates growth and nutrient status of salt-stressed cotton (Gossypium hirsutum) seedlings

Being macronutrient, K⁺ is involved in a number of metabolic processes including stimulation of over 60 enzymes. The present study was conducted to investigate whether K-priming could alleviate the effects of salinity on the growth and nutrient status of cotton seedlings. The seeds of two cotton cultivars, namely FH-113 and FH-87, were primed with solutions of three potassium sources (KNO₃, K₂SO₄ and K₂HPO₄) using three concentrations (0%, 1.25% and 1.5%) of each potassium source. After 1 week of germination, the seedlings were subjected to salinity (0 and 200 mM NaCl) stress. The results showed that salinity significantly affected growth and nutrients status of cotton seedlings. The K-priming alleviated the stress condition and significantly improved dry matter as well as nutrient uptake in cotton seedlings. Of the priming treatments pre-sowing treatment with KNO₃ (1.5%) was most effective in increasing shoot and root lengths and biomass of cotton seedlings. The seedlings raised from seed treated with KNO₃ (1.5%) showed varied accumulation of cations (Ca²⁺, Na⁺ and K⁺) and faced less oxidative stress irrespective of cotton cultivars under salt stress. The results suggested that pre-sowing seed treatment with KNO₃ (1.5%) might be recommended for synchronized germination and sustainable production of cotton crop under saline environments.     

渭北旱塬果园自然生草对土壤水分及苹果树生长的影响

土壤水分不足是渭北旱塬苹果生产中的首要问题。为了提高果园土壤贮水量,促进果树生长,该试验以果园清耕和人工生草(三叶草)为对照,探讨渭北旱塬果园自然生草(繁缕和牛繁缕群落)对土壤水分及苹果树生长的影响。结果表明:自然生草在苹果开花坐果期可提高0~80 cm土层土壤水分,在幼果膨大期和花芽分化期可提高0~120 cm土层土壤水分,在果实采前膨大期可提高0~160 cm土层土壤水分,而人工生草则降低了土壤水分。自然生草和人工生草主要影响0~80 cm土层土壤水分,且对0~40 cm土层土壤水分影响较大,对120 cm以下土层影响较小。自然生草的土壤蒸散量较人工生草和清耕分别减少了17.30和8.07 mm,单果重分别提高了15.46%和6.21%,产量分别提高了21.29%和6.10%,土壤水分利用效率分别提高了25.09%和7.64%。自然生草不但可提高果园土壤贮水量,而且可提高果实单果重与产量,渭北旱塬应积极推广果园自然生草。     

不同白榆品系对滨海盐碱地的改良效果及盐分离子的分布与吸收

为揭示不同白榆(Ulmus pumila L.)品系对滨海盐碱地土壤盐分的改良作用及盐分离子在土壤-白榆系统中的分布与吸收特征,筛选适宜在滨海盐碱地造林的耐盐白榆品系,以中度盐渍化生境下4年生的6种白榆品系(1,5,28,30,46,105号)为试验材料,采用野外取样与室内测试相结合的方法,研究了Na+、K+、Ca2+、Mg2+等盐离子在土壤及白榆品系各器官(根、茎、叶)中的分布特征。结果表明:(1)白榆可降低滨海盐碱地土壤中盐离子及全盐含量,不同白榆品系较对照的土壤全盐含量降低了55.0%～63.1%,30号白榆降幅最大。(2)不同白榆品系将Na+、K+、Ca2+、Mg2+优先积累到叶中,且叶中维持较高的K+/Na+、Ca2+/Na+、Mg2+/Na+比值,不同白榆品系通过建立新的离子平衡以适应盐胁迫环境。(3)不同白榆品系的离子吸收选择性系数均为SK,NaSCa,NaSMg,Na,其对K+的吸收选择性大于对Ca2+、Mg2+吸收选择性;种内差异导致不同白榆品系对Na+、K+、Ca2+、Mg2+吸收选择能力不同,28号白榆根系对K+的吸收性最强,5号白榆根系对Ca2+、Mg2+的吸收性最强。     

Small amounts of ammonium (NH$ _4^+ $) can increase growth of maize (Zea mays)

Nitrate (NO$ _3^ - $ ) and ammonium (NH$ _4^+ $ ) are the predominant forms of nitrogen (N) available to plants in agricultural soils. Nitrate concentrations are generally ten times higher than those of NH$ _4^+ $ and this ratio is consistent across a wide range of soil types. The possible contribution of these small concentrations of NH$ _4^+ $ to the overall N budget of crop plants is often overlooked. In this study the importance of this for the growth and nitrogen budget of maize (Zea mays L.) was investigated, using agriculturally relevant concentrations of NH$ _4^+ $ . Maize inbred line B73 was grown hydroponically for 30 d at low (0.5 mM) and sufficient (2.5 mM) levels of NO$ _3^ - $ . Ammonium was added at 0.05 mM and 0.25 mM to both levels of NO$ _3^ - $ . At low NO$ _3^ - $ levels, addition of NH$ _4^+ $ was found to improve the growth of maize plants. This increased plant growth was accompanied by an increase in total N uptake, as well as total phosphorus, sulphur and other micronutrients in the shoot. Ammonium influx was higher than NO$ _3^ - $ influx for all the plants and decreased as the total N in the nutrient medium increased. This study shows that agriculturally relevant proportions of NH$ _4^+ $ supplied in addition to NO$ _3^ - $ can increase growth of maize.     

盐胁迫下柚实生苗生长、矿质营养及离子吸收特性研究

以坪山柚为材料,对盐胁迫下实生苗生长、矿质营养及离子吸收特性进行了研究。结果表明,沙培30d,80～200mmol/L盐胁迫,随盐浓度提高,坪山柚实生苗株高、叶面积、地上部干重和根部干重明显降低。溶液培养8d,坪山柚实生苗地上部及根Na+、Cl-含量随盐浓度的增加而增加,根及地上部K+、Ca2+、Mg2+以及P和Mn含量下降,Fe、Zn、Cu含量的变化因器官而异。其中,地上部Fe含量对盐胁迫敏感,可作为柚耐盐性鉴定指标。40mmol/L盐胁迫,坪山柚地上部K+/Na+、Ca2+/Na+、Mg2+/Na+值均显著下降,且Mg2+/Na+值+/Na+值>1;浓度≥160mmol/L盐胁迫,K+/Na+值+吸收、运转效率比Cl-高。     

不同砧木对富士苹果矿质元素含量和品质指标的影响

砧木作为果树的重要组成部分,不仅影响树体对矿质营养元素的吸收、 运转和利用,而且对地上部树体的生长发育和果实品质形成具有重要的调控作用。本文以富士苹果为试材,研究了不同砧木与苹果叶营养元素、 果实矿质营养和品质指标间的关系。结果表明, 砧木类型影响苹果叶片对矿质元素的吸收,矮化砧苹果叶片的P、 K、 Ca和Cu含量均高于乔化砧,分别比乔化砧高20.00%、 13.50%、 9.09% 和 32.72%;但其他矿质元素含量均低于乔化砧。除Ca和B元素外,矮化砧苹果果实的N、 P、 K、 Mg、 Fe、 Mn和Cu含量均高于乔化砧,分别比乔化砧高39.46%、 46.30%、 19.13%、 9.68%、 32.41%、 18.96% 和 68.31%。与乔化砧相比,矮化砧富士苹果具有较高的单果重、 可溶性固形物和可滴定酸含量,分别比乔化砧高5.53%、 5.25% 和 6.82%。相关分析表明,苹果叶片大量元素 N、 P、 K和Ca与果实部分营养元素间存在显著的相关性,其中叶片K和果实N的相关系数高达0.9001;而叶片微量元素Fe、 Mn、 Zn和B与果实矿质元素间均不存在显著的相关性。另外,富士苹果果实的Ca含量仅为叶片Ca含量的1.58％,是所测矿质元素中相对含量最低的,这可能是套袋苹果易发生苦痘病的原因所在。     

施肥对饲草高粱生长及营养品质的影响

研究施用氮、磷,钾肥对饲草高粱生长及营养品质动态的影响结果表明,氮肥、磷肥和钾肥配合施用可大幅提高饲草高粱生物产量,增加饲草高粱植株粗蛋白质、粗纤维和粗脂肪的单位面积产出量;氮素是影响高粱生物产量的首要养分因素,其次为磷素,第三是钾素;饲草高粱植株高度以及粗蛋白质、粗纤维和粗脂肪含量的变化规律,主要取决于品种特性;施肥主要通过影响饲草高粱生物产量,对饲草高粱植株粗蛋白质、粗纤维和粗脂肪的单位面积产出量产生作用.从饲草高粱的生物产量和品质综合考虑,确定饲草高粱的适宜收割期,以开花末期至成熟中期之间为宜,过早收割饲草高粱生物产量较低,过晚收割则饲草高粱生物产量较低且品质变劣.     

Population Studies of Limonium stocksii (Plumbaginacea) from a Salt Desert Near the Arabian Sea Coast

Abstract

A population of Limonium stocksii was studied for one year at the upper end of Manora Creek along the Arabian Sea coast, near Karachi, Pakistan. The community was divided into three zones. Soil pH varied from 8.1 to 8.5. Soil conductivity was highest (191 dS m^?1) in September and lowest (55 dS m^?1) in June. Soil water content was relatively low and varied from 1.6% in February to 9.5% in June. Limonium stocksii maintained a persistent seed bank, which peaked at 5,887 seeds m^?2 in May. Very slow growth was observed; however, plants in the intermediate zone showed better growth than the other two zones. Stem and leaf tissue water content were affected by salinity fluctuations, but root tissue water remained unaffected. Sodium (Na⁺) and chloride (Cl^?) accumulated in stems and leaves more than other ions. Leaves also accumulated a very high amount of magnesium (Mg⁺⁺) compared to roots and stems.     

草被减流减沙效应及其力学机制分析

 利用人工模拟降雨试验,定量研究不同降雨强度下20°坡面草地的减流减沙效应,探讨草被坡面固土作用的力学机制。结果表明:在45、87和127mm/h降雨强度下,草地坡面土壤的平均入渗率是裸地坡面入渗率的2.1～4.2倍;与裸地相比,草地径流流速减少77.3%～79.8%,径流量减少51.9%～99.1%,产沙量减少93.6%～99.2%;从力学角度分析坡面土壤颗粒的受力情况,建立的坡面产沙量与径流切应力的关系模型可用于草被坡面土壤流失量预测;试验条件下,草地临界径流切应力值为2.857N/m2,裸地临界径流切应力值为0.861N/m2,坡面产沙量随径流切应力的增大而增大。研究结果对定量评价草被减流减沙作用和深化土壤侵蚀力学过程有一定的参考意义。     

草鱼消化道碱性蛋白酶的提取及鉴定

用丙酮将草鱼肠脱脂,磷酸盐缓冲溶液提取粗酶液,再用10%~40%饱和度的硫酸铵分级沉淀制取粗草鱼消化道碱性蛋白酶。粗草鱼碱性蛋白酶的比活力为2 800 U/g,最适作用温度为37°C,最适作用pH为10.3。在50°C保温30 min后还保留60%以上的酶活力,要使其损失约80%的活力,需要在60°C保温30 min。50 mmol/L的甲苯磺酰赖氨酸氯甲酮(TLCK)、甲苯磺酰氟(PMSF)和50μmol/L大豆胰蛋白酶抑制因子(SBTI)分别抑制78.92%、75.98%和80.23%的酶活力。SDS-底物-聚丙烯酰胺凝胶电泳表明碱性蛋白酶的活性成分有4种,一种为非丝氨酸蛋白酶,相对分子质量为105 000;另外三种为胰蛋白酶,相对分子质量分别为26 400、30 750和43 000。     

Effects of NaCl and NaHCO3 stress on morphological growth and nutrient metabolism on selected avocados (Persea americana Mill.)

The effect of alkali stress on crop production has gained importance around the world. Avocado (Persea americana Mill.) is considered a salt-sensitive species, but the effect of alkaline water on avocados has not been sufficiently studied. Plant growth, leaf damage, and chemical analysis were evaluated in response to alkali salt (NaHCO₃) and neutral salt (NaCl) stresses on six clonally propagated avocado rootstocks. All plants exhibited exclusion mechanisms by the accumulation of Na⁺ and Cl^? in their root systems, Na⁺ was concentrated to a greater extent than Cl^?. The accumulation of Na⁺ in the leaves was greater when applied as NaHCO₃ compared to the NaCl treatment. Although Cl^? toxicity is more commonly observed under usual field conditions, in our experiments when Na⁺ reached the leaves it caused nearly two times more leaf necrosis.     

再生水灌溉对土壤主要盐分离子的分布特征及盐碱化的影响

通过田间试验,研究再生水纯灌、轮灌、重要时期灌清水等不同灌溉策略对西红柿和黄瓜地块土壤主要盐分离子的分布特征及盐碱化的影响。结果表明:对于西红柿地块,除Cl-和HCO3-在表土中的发生积累外,其他主要盐分离子均强烈受到灌溉水淋溶作用的影响,表现出向土壤深层迁移的特征,但是再生水灌溉未引起土壤剖面盐分的累积。对于黄瓜地块,土壤盐分分布对不同再生水灌溉策略的响应不同于西红柿地块,强烈的蒸发作用造成了主要盐分离子(除HCO3-外)在土壤表层中出现积累,但是未监测到盐分在深层土壤中累积。再生水灌溉对西红柿和黄瓜地块土壤的pH值和SAR值均未产生显著影响,但是较高的pH值(>8.0)说明长期灌溉再生水可能引起土壤的碱化。研究认为重要时期灌清水处理在控制土壤盐分累积方面效果明显,且未引起土壤发生碱化,可作为短期再生水灌溉果蔬菜(西红柿、黄瓜)的有益尝试。     

有机肥与尿素配施对设施土壤盐分含量与组成变化的影响

通过室内模拟培养试验,研究了有机肥与尿素配施对设施土壤盐分的影响。结果表明：（1）设施土壤的含盐量和电导率均随着施肥后培养时间的延长,呈先升高后降低再升高的趋势,培养15～30d较高,30—60d降低,60d后又缓慢升高。设施土壤含盐量与有机肥施用量呈极显著正相关,而与尿素用量无明显的相关性;电导率则与尿素和有机肥施用量均有极显著正相关。（2）施用尿素不同程度增加了K＋、Na＋、Ca2＋、Mg2＋,NO3-的含量,施用有机肥显著增加了K＋、Na＋、Cl-的含量,设施和露地土壤盐分总量和离子组成差异不显著。另外研究了土壤盐分总量和电导率分别与K＋、Na＋、Ca2＋、Mg2＋、SO4^2-、Cl-等含量的相关性,均呈极显著正相关。     

不同形态氮肥对设施土壤盐分含量和离子组成的影响

通过室内模拟培养实验,研究了尿素、碳酸氢铵和硝酸钙对设施土壤盐分含量和离子组成的影响.结果表明:(1)随着培养时间延长,不同形态氮肥处理土壤含盐量总体呈先升高后降低再升高的趋势.施肥处理的土壤含盐量较对照(CK)明显增加,但不同氮肥处理与未施氮肥处理(N0)间的土壤含盐量在整个培养期间内总体差异不显著.且氮肥用量对含盐量的影响也不显著.(2)设施土壤中尿素和碳酸氢铵处理的电导率与含盐量呈极显著正相关,硝酸钙处理电导率与含盐量呈显著正相关.且随培养时间的延长呈波浪状变化.其电导率显著高于相同氮用量时的其他两种氮肥处理;电导率与氮肥用量呈极显著正相关.(3)不同形态氮肥处理的土壤盐分离子组成相近,均以阴离子为主.设施土壤阴离子占盐分总量的76.29%～93.81%,露地土壤为75.16%～96.63%,以SO_4~(2-)含量最高,分别占67.37%和67.28%,NO_3~-含量次之;土壤中阳离子含量较少,以Ca~(2+)为主,K~+,Na~+,Ca~(2+),Mg~(2+)的含量随氮肥用量的增加而增加.(4)土壤电导率受阳离子的影响较大.阴离子的影响较小.设施土壤的电导率与Ca~(2+),Mg~(2+),K~+.NO_3~-含量呈极显著正相关,与Cl~-1呈显著正相关.露地土壤电导率与Ca~(2+),Mg~(2+)含量呈极显著正相关,与HCO_3~-呈显著负相关.     

Effect of salt stress on Growth and Ion accumulation of alfalfa (Medicago sativa L.) cultivars

Alfalfa (Medicago sativa L.) yield and nutrient contents may be affected under salinity condition. Thus, this experiment was conducted to determine the effect of three salinity levels (60, 120, and 180 mM NaCl) on shoot and root dry weights, and mineral contents of three alfalfa cultivars. With the increasing salinity levels sodium (Na) and magnesium (Mg) contents increased; but potassium (K), nitrogen (N), phosphorous (P), calcium (Ca), zinc (Zn), and copper (Cu) contents and root and leaf weights decreased; however, changes in these traits depended on cultivar and salinity level. However, Rehnani, a tolerant cultivar, had the lowest Na and Mg contents and the highest K, N, P, Ca, Zn, and Cu contents and dry weights under all of the salinity levels. Moreover, leaf dry weight and leaf P content had the highest correlation with salt tolerance suggesting that these traits may be used as a marker for selecting salts that are tolerant among genotypes in alfalfa.