ALUMINUM EFFECTS ON GROWTH,PHOTOSYNTHESIS, AND MINERAL NUTRITION OF CACAO GENOTYPES

A solution culture experiment was conducted in a greenhouse with two cacao genotypes (Catongo and Theobahia). Plants were subjected to seven aluminum (Al) concentrations and Al effects on growth, photosynthesis, and mineral content were assessed. Inter-genotypic differences for F_o, F_m and F_v fluorescences, as well as for F_v/F_m, were observed with increasing Al concentrations. There was a decreasing of F_o, F_m, F_v and F_v/F_m for the Catongo. Increasing Al concentration decreased photosynthetic rate, stomatal conductance and leaf transpiration rate, however, inter-genotypic differences in these parameters were not observed. The Al-treatments decreased content of nitrogen (N), phosphorus (P), potassium (K), calcium (Ca), and magnesium (Mg) in different plant parts, with the exception of K content in stems of Theobahia and in leaves of both genotypes. Theobahia showed higher tolerance to Al phytotoxicity, manifested mainly in the higher levels of Al in the growth medium. Inter-genotypic differences were observed in the growth and mineral nutrition.     

CUMULATIVE AND RESIDUAL EFFECTS OF ZINC SULFATE ON GRAIN YIELD,ZINC, IRON,AND COPPER CONCENTRATION IN CORN AND WHEAT

Re-application of zinc (Zn) sulfate for corn (Zea mays L.) production in rotation of wheat-corn has varied effects on yield of crops grown in Zn deficient soils. Therefore, this study was done as split plots in a complete randomized block design (CRBD) where the main plots were control with and without Zn application in wheat (Triticum aestivum L.) production. Sub-plots were of control, without Zn fertilizer, base application of 75 kg per hectare (kg Zn ha^?1), 25% and 50% less than base application and as foliar spray in combination with the 4 soil Zn treatments for corn production. Effect of previous Zn application on grain Zn concentration of corn was significant (P < 0.01). Zinc concentrations in treatments of without previous Zn (nil Zn) application and with Zn application were 28.1 and 31.8 mg kg^?1, respectively. Soil application of 75 kg ha^?1 and foliar application of Zn sulfate gave the highest yield (8853 kg ha^?1) showed an increase of 25 percent in compared with nil-Zn. Although re-application of Zn has small effect on yield, but resulted in was the highest grain concentration.     

SILICON EFFECTS ON THE PARTITIONING OF MINERAL ELEMENTS IN SOYBEAN SEEDLINGS UNDER DROUGHT AND ULTRAVIOLET-B RADIATION

The aim of this study was to investigate the effect of silicon (Si) on soybean seedlings under (combined) drought and ultraviolet-B (UV-B) radiation stresses. Specifically, we determined dry matter accumulation and partitioning of the mineral elements in different organs of soybean seedlings using the inductively coupled plasma mass spectrometry (ICP-MS). The amount of dry matter accumulated in root, stem, and leaf of seedlings treated with (combined) drought and UV-B radiation stresses were lower than that of the control. Also, the content of macroelements in seedlings under the combined stress was lower than in those under control conditions. Changes in the contents of microelements varied according to the intensity of drought, UV-B radiation, and the organs of soybean. The relationship between the contents of mineral elements and the accumulation of dry matter also varied similarly. These results demonstrated that drought and UV-B radiation induced an alteration in the distribution of mineral elements in root, stem and leaf, leading to decreases in dry matter accumulation and inhibition of soybean growth.     

EFFECT OF THE ECTOMYCORRHIZAL FUNGUS PAXILLUS INVOLUTUS ON GROWTH AND CATION (POTASSIUM,CALCIUM, AND MAGNESIUM) NUTRITION OF PINUS SYLVESTRIS L. IN SEMI-HYDROPONIC CULTURE

A semi-hydroponic culture was used to compare growth and cation nutrition of mycorrhizal (Paxillus involutus) and non-mycorrhizal Scots pine seedlings. When roots and hyphae grew together, concentrations and contents of macronutrients in needles and roots were not significantly different between mycorrhizal and non-mycorrhizal plants. When grown in two separate compartments, root potassium (K) concentrations, concentrations and contents of calcium (Ca) in needles and roots, needle nitrogen (N) concentrations, total N content and contents of root K and Mg were significantly reduced in mycorrhizal plants. Whereas ¹⁵N abundance increased in roots of mycorrhizal plants. The results indicated that the extraradical mycelium of the fungus strain used was able to transport N to the plant but did not contribute to long-term cation uptake and growth of host plants. An insufficient supply of macro-elements [N, phosphorus (P)] may account for the reduced growth of mycorrhizal plants and the differences in cation uptake between mycorrhizal and non-mycorrhizal plants.     

EFFECT OF ZINC NUTRITION ON GROWTH,YIELD, AND QUALITY OF FORAGE SORGHUM IN RESPECT WITH INCREASING POTASSIUM APPLICATION RATES

A two-year field study was conducted to determine the effect of two zinc (Zn) levels [0 and 10 kg zinc sulfate (ZnSO₄) ha^?1] in respect with four potassium (K) levels (0, 20, 40 and 60 kg K₂O ha^?1) on growth, yield and quality of forage sorghum. The soil of the experimental field was loamy sand (Inceptisol), carrying 70, 08, 77, and 0.51 mg nitrogen (N), phosphorus (P), K, and Zn kg^?1 soil, respectively. Increasing K levels significantly improved most of the growth, yield, and quality attributes gradually irrespective of the Zn levels. Zinc applied at 10 kg ZnSO₄ ha^?1 proved significantly better than no zinc application at various K application rates. The benefit of zinc application increased progressively with increasing K rates for most of the parameters studied, indicating significant response of the crop to positive K × Zn interaction in plants in respect with K and Zn application to the soil. Accordingly, 60 kg K₂O ha^?1 applied with10 kg ZnSO₄ ha^?1 boosted most of the attributes maximally. It resulted in about 20–40% increase in growth attributes, 25% increase in fresh matter yield, 36–38% increase in dry matter yield, and 38% increase in protein yield compared to the comparable K level applied without zinc. It also enhanced N uptake by 38%, P uptake by 5–19%, K uptake by 40–42%, and Zn uptake by 114–144%. Across the K rates, application of 10 kg ZnSO₄ surpassed no zinc application by 30–35% in N uptake, by 8–15% in P uptake, by 33–36% in K uptake, by 120–140% in Zn uptake, by 19–21% in fresh matter yield, by 29–31% in dry matter yield, and by 30–34% in protein yield.     

石灰性土壤施磷、铁对柠条生长及根际土壤环境的影响

采用随机区组和裂区设计,通过盆栽和根箱模拟试验研究了石灰性土壤,在水分充足的条件下施磷及磷、铁对柠条生长发育及根际土壤养分有效性的影响。盆栽试验结果表明,柠条的生物产量随着施磷水平的增加而增加;在低磷或磷胁迫条件下,柠条的地上部生长受到抑制,根冠比增大,土壤pH值迅速降低。根箱模拟试验发现,不同的铁、磷施肥配比对柠条生物产量的影响不同,当磷和铁的施用量分别为P2O50.15 g kg-1 和FeSO4·7H2O0.03g kg-1时能明显提高柠条的生物量。不同铁、磷配比对柠条根际土壤有效磷含量影响的根际范围是0-6 mm之间,在此范围内供试土壤有效磷含量随距离快速下降,并与根际土壤pH值呈反比。柠条对根际土壤pH的调控主要受磷水平的影响,而施铁水平对根际和根外土壤pH值的影响比较小。     

海水养殖废水灌溉条件下SPAC系统中水盐肥通量研究

2004年4月～10月，在半湿润的莱州地区布置蒸渗试验，研究海水养殖废水灌溉条件下SPAC系统水盐肥通量。试验设5个处理，分别为CK（种作物，不灌溉）和淋洗分额（LF）为0．1、0．2、0．3、0．4的海水养殖废水灌溉处理。结果表明：对于淋洗分额为0．1、0．2、0．3、0．4的海水灌溉处理，用于土壤蒸发和作物蒸腾的海水养殖废水量分别占菊芋生育期总蒸散量的36．5％、36．2％、37．0％、37．3％；对于上述海水养殖废水灌溉处理，土壤耕层的盐分积累量分别为91．1、94．1、98．7、107．1g；而铵态氮的积累量分别为2．00、2．29、2．27、2．82mg，硝态氮的积累量分别为1．81、1．40、1．29、0．92mg，活性磷酸盐的积累量分别为3．03、2．68、2．44、1.67mg。因此，认为海水养殖废水灌溉在供给作物水分和养分方面起到积极作用，但需采取调控措施以防耕层土壤盐分过量积累。     

STUDY ON THE EFFECTS OF NITROGEN DEFICIENCY ON GRAIN IRON AND ZINC CONCENTRATIONS OF LANDRACE AND BRED WHEAT VARIETIES IN CHINA

It has been reported that nitrogen (N) deficiency can significantly reduce grain iron (Fe) and zinc (Zn) concentrations in wheat. Here, we investigated the grain Fe (GFeC) and Zn (GZnC) concentrations of 42 Chinese wheat varieties grown in a field trial under low and high N conditions. Analysis of variance revealed that both GFeC and GZnC significantly differed among varieties and between N treatments, and significant interactions existed between variety and N treatment. The GFeC under the low N condition was 94.8% of that under high N condition, ranging from 79.4% to 112.7%; the GZnC under low N condition was 74.8% of that under high N condition, ranging from 59.5% to 102.5%. Wheat varieties with high GFeC and GZnC under both N conditions were identified, and this kind of varieties is desirable in breeding wheat with enriched Zn and Fe under a range of soil N conditions.     

大豆黄酮对产蛋鸡生产性能、血液指标及激素分泌的影响

选用44周龄海兰褐商品蛋鸡208只,随机分为4组,分别在基础日粮中添加大豆黄酮0、5、10和15mg/kg,研究了日粮中添加不同水平大豆黄酮对不同时期产蛋鸡生产性能及相关激素水平、血液生化指标及激素分泌的影响。结果表明:添加大豆黄酮在试验各期均可以提高产蛋率、改善料蛋比,15mg/kg组可以显著提高日产蛋量;10和15mg/kg组可以显著提高游离三碘甲状腺原氨酸(FT3)水平,极显著提高游离甲状腺素(FT4)水平;5mg/kg组极显著降低血清IGF-1含量,15mg/kg组极显著提高血清IGF-1含量,各处理组血清胰岛素水平均极显著高于对照组;随着大豆黄酮添加量的增加,血清总胆固醇、甘油三酯、血清低密度脂蛋白胆固醇、血清高密度脂蛋白胆固醇、血糖、血清尿酸呈递减趋势。大豆黄酮可以明显改善44-56周龄海兰褐蛋鸡的生产性能、内分泌激素水平,并降低血液中胆固醇含量。     

铁和不同形态氮素对玉米植株吸收矿质元素及其在体内分布的影响 Ⅱ.对铁、锰、铜、锌等营养元素的影响

用营养液培养方法研究了铁和两种形态氮素对玉米植株吸收铁、锰、铜、锌等微量元素及其在体内分布的影响。结果表明:与硝态氮(NO3--N)相比,铵态氮(NH4+-N)显著提高了玉米对铁的吸收,降低了对锰、铜及锌的吸收。供铁也明显提高了植株地上部铁的吸收总量,降低了锰及锌的吸收量,尤其是在供应No3--N时这种作用更为明显。在缺铁条件下,NH4+-N处理的玉米新叶中铁的含量明显高于NO3--N处理;而新叶、老叶、茎中锰、锌、铜含量以及根中锰、锌含量都明显低于NO3--N处理。但使用NH4+-N时,根中铜的含量较高。在供铁条件下,NH4+-N处理的玉米植株四个不同器官中锰和锌的含量显著低于NO3--N处理的植株,而铜的含量正好相反。在缺铁条件下,玉米新叶中活性锰、活性锌的含量显著高于供铁处理;与NO3--N相比,NH4+-N的供应也显著降低了玉米新叶中活性锰以及活性锌的含量。     

铁和不同形态氮素对玉米植株吸收矿质元素及其在体内分布的影响 Ⅱ.对铁、锰、铜、锌等营养元素的影响

用营养液培养方法研究了铁和两种形态氮素对玉米植株吸收铁、锰、铜、锌等微量元素及其在体内分布的影响。结果表明:与硝态氮(NO₃^--N)相比,铵态氮(NH₄⁺-N)显著提高了玉米对铁的吸收,降低了对锰、铜及锌的吸收。供铁也明显提高了植株地上部铁的吸收总量,降低了锰及锌的吸收量,尤其是在供应No₃^--N时这种作用更为明显。在缺铁条件下,NH₄⁺-N处理的玉米新叶中铁的含量明显高于NO₃^--N处理;而新叶、老叶、茎中锰、锌、铜含量以及根中锰、锌含量都明显低于NO₃^--N处理。但使用NH₄⁺-N时,根中铜的含量较高。在供铁条件下,NH₄⁺-N处理的玉米植株四个不同器官中锰和锌的含量显著低于NO₃^--N处理的植株,而铜的含量正好相反。在缺铁条件下,玉米新叶中活性锰、活性锌的含量显著高于供铁处理;与NO₃^--N相比,NH₄⁺-N的供应也显著降低了玉米新叶中活性锰以及活性锌的含量。     

ADDITION OF PULP MILL ASH RAISES PH,MODIFIES PHYSICAL PROPERTIES,AND ALTERS YOUNG TOMATO PLANT GROWTH AND MINERAL NUTRITION IN A PEAT-BASED SUBSTRATE

Pulp and paper mills often burn wood waste to fuel their boilers. The ash from a Mississippi pulp mill boiler was evaluated for potential use as an amendment to peat moss-based greenhouse substrates for production of young tomato plants. Between 0 and 50% ash was added to a custom-blended peat moss-based substrate, and these were compared to a commercially available substrate without ash. Addition of ash increased substrate pH, conductivity (EC), bulk density and water holding capacity, while reducing airspace and average particle size. In general, tomato plants grown in 0–40% ash had similar growth indexes as plants grown in commercial substrate. Increasing amounts of ash decreased tomato shoot nitrogen (N), potassium (K), iron (Fe), manganese (Mn), zinc (Zn), and copper (Cu) concentration, and increased concentrations of phosphorus (P), calcium (Ca), sulfur (S), and boron (B). These results indicate that pulp mill ash has the potential to be used as a substrate component for greenhouse container production of tomato.     

正常和高铵条件下氯化钠对万寿菊生长、开花和代谢的影响

在温室中以养分平衡的肥料组合和含250mg/L NH_4~+的组合栽培万寿菊(Tagetes patula L.)。每种组合中以一半植株每天每盆加250 mL 27.5 mmol/L NaCl溶液,进行盐胁迫处理。盐处理结果,使每株鲜重、株高和开花数都减少。盐处理2周后,根、叶细胞膜相对透性显著增加,叶子热处理渗出物中游离NH_4~+、根渗出物中Na~+。以及叶子中的过氧化物酶、Mg~(2+)-ATP酶和Ca~(2+)-ATP酶活性都明显提高,而根、叶渗出液的pH和蛋白质含量显著降低。根渗出物中游离NH_4~+、K~+和Ca~(2+)含量,叶子中叶绿素、可溶性蛋白质、无机磷和多酚氧化酶活性也下降,但未发现叶子渗出物中Na~+有所增加。在正常营养条件下,盐胁迫对叶子中的类胡萝卜素和花青素影响较小,但高NH_4~+处理使这些色素含量显著降低。高NH_4~+一般使NaCl的效应加剧。     

铁和不同形态氮素对玉米植株吸收矿质元素及其在体内分布的影响——Ⅰ.对氮、磷、钾、钙、镁等营养元素的影响

用营养液培养方法研究了铁和两种形态氮素(NO3--N和NH4+-N)对玉米植株吸收氮、磷、钾等大量元素和钙、镁等中量元素及其在体内分布的影响。结果表明:与NO3--N相比,供应NH4+-N促进了玉米对氮的吸收,在缺铁条件下,降低了对磷、钾、钙及镁的吸收。铁和NH4+-N都显著提高了玉米植株各器官中氮的含量。与NH4+-N处理相比,NO3--N处理的新叶中磷含量显著增加,但铁的供应对植物体内磷的含量无显著影响。使用NO3--N显著提高了玉米新叶和老叶中钾的含量,根和茎中钾的含量无明显影响。铁的供应降低了新叶和老叶中钾的含量。供铁时,NH4+-N处理的玉米新叶中钙和镁的含量显著低于NO3--N处理,而在缺铁时则无显著差异。     

铁和不同形态氮素对玉米植株吸收矿质元素及其在体内分布的影响——Ⅰ.对氮、磷、钾、钙、镁等营养元素的影响

用营养液培养方法研究了铁和两种形态氮素(NO₃^--N和NH₄⁺-N)对玉米植株吸收氮、磷、钾等大量元素和钙、镁等中量元素及其在体内分布的影响。结果表明:与NO₃^--N相比,供应NH₄⁺-N促进了玉米对氮的吸收,在缺铁条件下,降低了对磷、钾、钙及镁的吸收。铁和NH₄⁺-N都显著提高了玉米植株各器官中氮的含量。与NH₄⁺-N处理相比,NO₃^--N处理的新叶中磷含量显著增加,但铁的供应对植物体内磷的含量无显著影响。使用NO₃^--N显著提高了玉米新叶和老叶中钾的含量,根和茎中钾的含量无明显影响。铁的供应降低了新叶和老叶中钾的含量。供铁时,NH₄⁺-N处理的玉米新叶中钙和镁的含量显著低于NO₃^--N处理,而在缺铁时则无显著差异。     

ZINC,MANGANESE AND PHOSPHORUS INTERRELATIONSHIPS AND THEIR EFFECTS ON IRON AND COPPER IN CHELATOR-BUFFERED SOLUTION GROWN RUSSET BURBANK POTATO

Three hydroponic experiments were conducted with Russet Burbank potato to elucidate zinc (Zn) and manganese (Mn) relationships and associated interactions with other nutrients at different levels of phosphorus (P). Except when P was optimal, root Mn concentration was reduced at optimal solution Zn relative to deficient or excessive Zn levels. Shoot Mn generally increased with augmented solution Zn. As solution Zn increased, root P declined while shoot P and copper (Cu) and root iron (Fe) and Cu increased. Increasing solution Mn generally decreased Zn in all plant tissues—especially in roots. The Mn influence on plant P, Cu, and Fe concentrations was nearly opposite the Zn impact, indicating that Zn and Mn have counter effects. Partitioning between root and shoot micronutrient concentrations occurred when available P was either deficient or excessive, which may also help explain poor plant health and adverse micronutrient relationships in potato when available P is not optimally maintained.     

太湖地区氮肥用量对土壤供氮、水稻吸氮和地下水的影响

利用矿化培养与田间试验的方法,探讨了太湖地区长期施氮条件下,氮肥用量对土壤供氮、水稻吸氮与环境的影响。初步试验结果表明:多年施用氮肥能够提高土壤的供氮能力,并随施N量的增加而增加。增加氮肥用量能够提高稻株含氮量和吸氮量,但氮素向谷粒的转移率降低,试验区水稻氮肥用量以225～270kghm-2左右为佳。稻田田面水和渗漏液的N素养分动态变化显示,施N会造成田面水NH4 N和NO3-N含量的短暂升高,但不同施N量之间相差并不显著。稻田渗漏液中的氮以硝态氮为主,通常在淹水泡田后的7d内有一个NO3-N含量的峰值期,NO3-N含量在1.62～2.75mgL-1之间,约10d后降至0.5mgL-1以下;NH4 N含量变化有随施N量而增加的趋势,高峰期通常出现在分蘖末期,其余时间NH4 N含量在0.2mgL-1以下。     

施肥对生土地谷子根苗生长及根际土壤酶和微生物种群的影响

以黄土母质性生土为供试土壤,采用盆栽试验,探讨了氮、磷、钾及有机肥对谷子生长发育的影响。结果表明:(1)磷是生土地土壤肥力形成与作物生长发育以及根际土壤酶系统和微生物系统形成的首要原始起动营养元素;(2)含磷的处理组合,谷子幼苗阶段地上下部生长均明显较不施磷的长势强,而单施氮和钾甚至不及不施肥的对照。氮、磷、钾对谷子根苗生长的影响顺序为磷氮钾;(3)进入谷子中后期生长阶段,氮素的作用,特别是氮、磷、钾对谷子株高及地上部干重的综合效果表现明显;(4)产量表现为含磷的处理组合,产量及产量结构均明显提高;(5)单施优质高量的有机肥对生土地根苗生长与产量形成具有当年立竿见影的效果;(6)发达的根系促进了根际微生物群落的迅速繁衍,提高了根际土壤酶的活性,有益于促进根-土系统向着熟化方向发展,使生土地上的谷子当年获得正常产量。     

SOME DELETERIOUS EFFECTS OF LONG-TERM SALT STRESS ON GROWTH,NUTRITION, AND PHYSIOLOGY OF GERBERA (GERBERA JAMESONII L.) AND POTENTIAL INDICATORS OF ITS SALT TOLERANCE

Tolerance of gerbera (Gerbera jamesonii L.) to long-term sodium chloride (NaCl) salt stress was evaluated by subjecting plants to 0, 10, 20, 30 and 40 mM NaCl levels for ten weeks. Increased NaCl led to a significant decrease in leaf and stem biomass. Salt stress significantly affected sodium (Na⁺), potassium (K⁺) concentrations in leaves, stems and roots leading to sharp declines in K⁺/Na⁺ ratios. Magnesium concentrations in stems and roots also showed significant declines. Adverse effect of salt stress on chlorophyll content was also significant. Proline seemed less effective in osmotic adjustment under long-term high salt stress. Switching from vegetative to reproductive growth phase was crucial for certain physiological functions. Leaf Na⁺ concentration showed significant correlation with important traits. These data suggest that NaCl threshold level in irrigation water for gerbera is around 10 mM. Leaf fresh weight, chlorophyll content and leaf K⁺/Na⁺ ratio are promising indicators of salt-sensitivity of gerbera.     

EFFECT OF BORON-DOPED GOETHITE ON SOIL ACIDITY,DIFFERENT FORMS OF MANGANESE IN RED SOIL AND THE GROWTH OF RAPE (BRASSICA NAPUS L.) SEEDLINGS

Rape (Brassica napus L.) seedling pot experiments were performed with a red soil treated with goethite which had boron (B) either adsorbed (ad-B-goethite) or occluded (oc-B-goethite). Soil acidity, different forms of manganese in the soils and different elements content of the rape seedlings were determined. It was found that the addition of boron-containing goethite to the soils resulted in increased rape growth, elevated soil pH and decreased exchangeable acidity. Compared with the control, boron-containing goethite elevated the content of exchangeable manganese (Mn) (EXC-Mn), organic matter bound Mn (OM-Mn), reducible oxide Mn (RO-Mn) and residual Mn (RES-Mn) which were difficult to use for plant. Low labile organic matter was significantly correlated with easily reducible oxide Mn (ERO-Mn) (P < 0.01) and RO-Mn (P < 0.05). Middle organic matter and soil pH was significantly (P < 0.05) correlated with RES-Mn. Stepwise regression was used to select the combination of variables that best estimates shoot and root dry weight of rape seedling. Among them, soil pH, EXC-Mn, OM-Mn, RO-Mn and RES-Mn significantly influenced the dry weight of rape seedlings. The addition of boron-containing goethite improved the uptake of iron (Fe), calcium (Ca), magnesium (Mg), and copper (Cu) element and decreased the uptake of Mn and zinc (Zn) element in rape seedling. The results suggested that boron-containing goethite could provide a better soil acidity environment for plant growth; it was also an important agent increasing a part of manganese difficult to use for plant and reducing the activity of soil manganese, which was beneficial to altering rape seedling growth.