Interaction of biological nitrogen fixation with sowing nitrogen fertilization on common bean in the two seasons of cultivation in Brazil

Sowing nitrogen (N) fertilization can limit biological nitrogen fixation (BNF) reducing common bean yield. The aim of this study was to evaluate the effect of sowing N fertilization plus inoculation on the growth and yield of plants in the two seasons of cultivation in Brazil. In the dry season, N fertilization and inoculation promoted a greater shoot dry weight and higher pod number and yield than only inoculated. In contrast, in the rainy season, this treatment promoted no increase in shoot dry weight and yield compared with the inoculated alone. The number of nodules was greater for the inoculated alone treatment, but nodule weight was not affected by N fertilization in either season. Therefore, sowing N fertilization and inoculation can be an agronomic practice to achieve a higher common bean yield in the dry season, while in the rainy season, the inoculation without N fertilization can support a high yield at a lower cost.     

氮磷配施对“济麦22”小麦产量及品质的影响

通过田间试验研究了氮磷配施对超高产冬小麦"济麦22"产量及磨粉品质、糊化特性、面团流变学特性及烘焙品质等的影响。结果看出,容重、出粉率与磨粉品质呈显著正相关;峰值粘度和峰值时间对面粉糊化特性影响显著;湿面筋含量、沉降值与面团流变学特性指标呈显著或极显著正相关,且对烘焙品质的影响显著。产量和品质的大部分指标随施氮磷量的增加而发生显著变化,氮磷肥对产量和品质的互作效应显著。施N 300 kg/hm2、P 150kg/hm2处理可获得超高产,且容重、出粉率、湿面筋含量、沉降值及面团稳定时间均显著高于其它处理,表现出较好的磨粉品质和烘焙品质。表明在本试验条件下,该施肥量是济麦22优质超高产的最佳施肥模式。     

Foliar phosphorus fertilization of common bean complementing phosphate fertilization at sowing

The objective of this study was to evaluate the response of common bean plants to phosphorus (P) applied to the leaves at different growth stages, as a complement to phosphate fertilization at sowing. The experiment followed the 2 × 7-factor randomized block design (RBD) with four replicates. The first factor was fertilization with P at the base with 60 and 120 kg ha^?1 of phosphorus pentoxide (P₂O₅). The second factor was the times and doses of P applied to the leaves: 0.5 or 1 kg ha^?1 of P₂O₅, at the V3 phenological stage; parceled in the following manner: 20% in V3, another 40% in R5 and 40% in R6, or 50% in R5 and 50% in R6; and one treatment with no P₂O₅ application to the leaves. P-based foliar fertilization increased P content in the grains of plants grown in soils with greater P availability. The lower common bean biomass production under scarce P availability was minimized by the foliar fertilization, as well as its productivity when 1 kg ha^?1 of P₂O₅ was applied to the leaves, 50% in the R5 phenological stage and 50% in R6. The highest productivity was obtained with the application of 0.5 kg ha^?1 of P₂O₅ to the leaves in the V3 phenological stage with the 120 kg ha^?1 dose at the base.     

Interaction effects of nitrogen and phosphorus fertilizer on nitrogen mineralization of wheat residues in a calcareous soil

Abstract

Because of the high pH of the soil in semiarid regions, phosphorus adsorption is unfavorable. So, considerable amounts of phosphorus fertilizers are used annually, where this fertilizer may affect the plant residues' decomposition. To examine the interaction effects of nitrogen and phosphorus on nitrogen mineralization in calcareous soil, a factorial experiment was performed in a completely randomized design with three replications. The first factor consisted of various C:N ratios (20, 40, and 60 or three levels of nitrogen N₁:0, N₂:11, and N₃:43?kg N ha^?1, respectively) and the second factor consisted of various C:P ratios (87, 174, and 260 or three levels of phosphorus P₁:0, P₂:12, and P₃:45?kg P ha^?1, respectively), under incubation conditions. The results indicated that the cumulative mineral nitrogen content in all treatments, except for N1P2 and N1P3 treatments, started from a positive amount and remained positive until the end of the incubation period. The highest amount of cumulative mineral nitrogen among treatments was related to N3P1 treatment, while the lowest was associated with N2P3 treatment. Mineralization of nitrogen during 60?d of incubation was the dominant phenomenon, except for the N1P2 and N1P3 treatments which remained in the organic phase. The effect of phosphorus on the cumulative mineralization of nitrogen was significant. With increasing the amount of phosphorus, the total inorganic nitrogen diminished. Nitrogen release begins earlier with lower C:N ratios, and therefore the available nitrogen can be released more quickly to the plant. It is generally concluded that, in calcareous soil, the use of nitrogen fertilizer to adjust C:N ratio and to improve the mineralization of wheat residues will be a suitable option.     

黄土高原氮磷肥水平对旱作冬小麦产量与氮素利用的影响

为了探求氮磷肥合理和高效利用的科学依据,以黄土高原中南部的旱地农田生态系统长期定位试验为基础,选取了不同施氮和施磷水平,探讨了不同降水年份,氮磷肥用量对作物产量、氮素吸收和利用等相关指标的影响。结果表明,平水年份施氮水平在0～45?kg/hm2和干旱年份在0～90?kg/hm2范围内作物产量和收获指数均随施氮水平的增加迅速提高,超过这个范围后则差异较小;施磷水平在0～45?kg/hm2之间产量增幅较大,干旱年份施磷量反而降低了收获指数;吸氮量随氮磷用量变化与产量有相似趋势;不同降水年份施氮水平对氮素收获指数均无显著影响,而施磷水平在干旱年份对氮素收获指数没有显著影响,平水年份则随施磷水平增加而有所提高;平水年份氮素利用效率随施氮水平的增加呈降低趋势,而干旱年份施氮水平对冬小麦氮素利用效率没有显著影响,施磷则在不同降水年份均比不施磷肥的处理提高了冬小麦的氮素利用效率,但较高的施磷水平之间差别较小。冬小麦产量和吸氮量之间有显著相关关系,干旱年份更接近二次曲线,氮素利用效率随吸氮量增加呈直线下降趋势。旱作农田氮磷配施及采用适中的氮磷肥用量可以促进作物对氮素的吸收,同时得到较高的氮素利用效率,从而使冬小麦获得较高的产量和较强抵御干旱的能力。     

Analysis of yield variability in winter wheat due to temporal variability,and nitrogen and phosphorus fertilization

Abstract

Field average based recommendations have been a common practice for recommending the major crop nutrients nitrogen (N) and phosphorus (P). The problem is yield will not be the same from year to year with application of the same amount of recommended rate of fertilizer. The objectives of this study were to demonstrate how recommendations generated using nutrient response experiments were dynamic; and to assess the relative contribution of temporal variability, N and P fertilizers on winter wheat grain yield and N concentration. Twelve factorial combinations of four N (0, 56, 112, and 168 kg ha^?1) and three P (0, 14.5, and 29 kg P ha^?1) rates were evaluated in a randomized complete block design with three replications at Perkins, Oklahoma. To address the first objective, ANOVA and orthogonal polynomial contrasts were used. To address the second objective, a ten predictor variable multiple linear regression model with two quantitative variables and their interaction (N, P and N×P) and seven-year variables was evaluated and a reduced model containing seven variables was generated. Wheat grain yield showed three distinct responses to N rates: Linear, quadratic and no response. These individual year data show that it is not always appropriate to use results of nutrient response experiments to estimate next year's N fertilizer requirement due to apparent temporal variability in the results. Wheat only responded to P during the first two years of the study. The reduced model from the regression analysis revealed that most of the variability in grain yield was accounted for by five individual indicator years and N only. High variability across years in grain yield and fertilizer (N and P) response, even between years of similar grain yield, is an indication of a given season's production dependence on factors other than N and P.     

Phaseolus vulgaris-rhizobia symbiosis increases the phosphorus uptake and symbiotic N2 fixation under insoluble phosphorus

Five north Tunisian native rhizobia were tested in symbiosis with ‘Coco-blanc’ variety under two phosphorus (P) supplies [P-sufficient and insoluble P (IP)] at 250 μmol P plant^?1. At flowering stage, responses against IP were assessed and indicated that inoculation with P.Ps.09 produced a high nodule biomass (0.12g). Similarly, P.Tb.09 produced 0.08 g nodule biomass under both P and P.Bj.09 that significantly increased nodule number under IP. Shoot biomass exhibited high dry weight with P.Tb.09 and CIAT899 (1.6 g plant^?1). Inoculation with CIAT899 produced a high root biomass under both P. Analysis of a halo diameter of clear zone rounding each colony under IP indicated that P.Tb.09 has developed high halo Ø (1.7cm). These variations were associated under IP with a decrease of pH by P.Ar.09 and with increase in shoot phosphatase activity by P.Ar.09, P.Bj.09, P.OM.09 and P.Ps.09. The use of P-efficient rhizobia may constitute an adaptive mechanism against P-deficiency tolerance.     

Interaction effect of nitrogen,phosphorus, and zinc fertilization on growth,yield, and nutrient contents of aromatic rice varieties

Abstract

Studies on nutrient interactions in aromatic rice are needed for proper understanding of impact of imbalanced use of nutrients in the era of multi and micro-nutrient deficiencies. A pot experiment was conducted during the rainy/wet season (June–October) of 2013 at New Delhi, to study the interaction effects of two levels each of nitrogen (N) (0 and 120?kg?ha^?1), phosphorus (P; 0 and 25.8?kg?ha^?1), and zinc (Zn; 0 and 5?kg?ha^?1) in two aromatic rice (Oryza sativa L.) varieties, viz. Pusa Rice Hybrid 10 and Pusa Basmati 1121. Application of N, P, and Zn resulted in increase of dry matter (0.91, 0.32, and 0.24?g plant^?1, respectively) 60?days after sowing (DAS) and grain yield of rice (3.68, 1.67, and 1.17?g plant^?1). The increase in yield of rice owing to N application was relatively higher by 0.98, 0.22, and 1.05?g plant^?1, respectively, when either P or Zn or both were applied with N than alone application of N, indicating synergetic effect of P and Zn application with N. The higher concentration and uptake of K in grain (0.25% vs 0.10%) and straw (1.32% vs 0.94%) were observed in the treatment received N than no N, though K was applied uniformly in all the treatments. It indicates positive interaction of N and K. The higher uptake of P in grain and straw was observed when P was applied with N and Zn (3.34 and 2.15?mg plant^?1), or with N (3.26 and 2.11?mg plant^?1) signifying positive effect of N on P uptake in rice.     

Effect of Sodium Chloride on Growth,Nutrient Accumulation,and Nitrogen Fixation of Common Bean Plants in Symbiosis with Isogenic Strains

The effect of sodium chloride (NaCl)-salinity on growth responses and tissues organic solutes and mineral content was investigated in common bean plants inoculated with salt-tolerant Rhizobium tropici wild-type strain CIAT899 and four mutant derivatives having decreased salt-tolerance (DST). Under non-saline conditions two mutants formed partially effective (HB10, HB12) and another two almost ineffective (HB8, HB13) nodules. A great variation of NaCl tolerance in the different symbiosis tested was observed at harvest, 32 day after planting. Common bean plant responded to salinity by decreasing the content of dry plant biomass, nodule number and the nitrogen fixation, and increasing the root to shoot ratio. The salt dose of 25 mM produced an increase of total soluble sugar and free amino acids content. This result suggest that these metabolites might be related with a nodule osmotic adjustment response under saline conditions, however cannot be excluded that the increase of amino acids content could be a consequence of protein degradation. In the other hand, sodium, calcium and phosphorus contents in shoot increased under the saline treatments. Potassium (K) and calcium (Ca) contents, unlike phosphorus (P) content, in shoot were not related with the symbiotic efficiency of mutant, however the decrease of P content suggest that these symbioses have limited their P absorption process independently of the saline treatment. NaCl tolerance associated with a retention of sodium and maintenance of potassium selectivity seem to be a strategy used for the salt stressed common bean plants in symbiosis assayed here.     

黑、紫粒小麦对氮素吸收及同化特性的初步研究

以3个不同类型小麦品种为试材,通过对幼苗期根系氮素吸收Vmax和Km的测定及灌浆期剑叶中NR、GS活性的比较分析,初步探讨了黑、紫粒小麦根系对氮素吸收的动力学和同化特性及其与营养品质间的相互关系。结果表明,同普通的白粒小麦相比,黑、紫粒小麦在对氮素的吸收、转运和同化等方面均具有较强的优势,这可能是其蛋白质等营养成分富积的主要原因之一。     

Importance of iron use‐efficiency of nodules in common bean (Phaseolus vulgaris L.) for iron deficiency chlorosis resistance

Several studies suggest that the Fabaceae‐Rhizobium symbiosis is particularly sensitive to iron (Fe) deficiency with respect to NO₃^–‐dependent plants. The aim of this study, which is part of a screening program for common bean tolerance to Fe deficiency, was to study genotypical differences in Fe requirement and Fe use‐efficiency of common bean cultivars depending on symbiotic nitrogen fixation (SNF). Results show that ARA14 produces more whole plant dry matter and particularly more nodule biomass than Coco blanc. ARA14 is characterized by a high capacity of nitrogen fixation and a better Fe use‐efficiency for the growth and the function of the nodules.     

Nodule phosphorus requirement and O2 uptake in common bean genotypes under phosphorus deficiency

Abstract

The effect of P deficiency on nodulation, nodule P content, nodule O₂ permeability and N fixation rates in Phaseolus vulgaris–rhizobia symbiosis was studied under glasshouse conditions. Four recombinant inbred lines (L34, L83, L115 and L147) and one variety cultivated in Morocco (Concesa) were inoculated with Rhizobium tropici CIAT 899 in hydroaeroponic culture. Two P levels i.e. 75 (deficient level) and 250 µmol plant^?1 week^?1 P (sufficient level) were applied and the trial was assessed 42 days after transplanting that coincide with plant flowering stage. Under P-deficiency, decrease of plant growth (18%) and nodule biomass (19%) was detected and significantly pronounced in the sensitive line L147 compared with the remaining genotypes. Additionally, under P-deficiency, the efficiency in use of rhizobial symbiosis, estimated by the slope of the regression model of shoot biomass as a function of nodule biomass, was significantly increased in the four lines. This constraint did not significantly influence nodule P content in Concesa, but it was 24 and 41% lower in the tolerant and in the sensitive lines, respectively. Nodule P content was positively correlated to nodule biomass, r=0.75, and shoot N, r=0.92. These genotypic variations were associated with variability in nodule O₂ permeability that was significantly affected by the P level-bean genotype interaction. Under P-deficiency, nodule O₂ permeability was significantly reduced in the tested genotypes and accompanied with a decrease in shoot N content, especially in the sensitive lines (35%). Moreover, the ratios plant N fixed: nodule P content and plant N fixed:nodule dry weight were affected under P-deficiency in four lines with an exception observed in Concesa. Depending on the observed data we concluded that N₂ fixation efficiency could be influenced by nodulation and level of nodule P requirement which depend on both bean genotypes and P level.     

施氮量对小麦氮代谢相关酶活性和子粒蛋白质品质的影响

在2003～2004年和2004～2005年小麦生长季,以强筋小麦济麦20为材料,分别设置N 0、96、168、240、276 kg/hm2 5个施氮量处理和0、96、168、240 kg/hm2 4个施氮量处理,研究不同施氮量对小麦氮代谢相关酶活性和子粒蛋白质品质的影响。两年度的试验结果均表明,在一定施氮量范围内,随施氮量增加,公顷穗数、穗粒数、蛋白质含量、子粒产量和蛋白质产量均显著升高;继续增加施氮量子粒产量显著降低,公顷穗数、穗粒数、蛋白质产量降低或无显著差异。其中2004～2005年生长季,在0～168 kg/hm2施氮量范围内,随施氮量增加,旗叶谷氨酰胺合成酶（GS）活性、开花21d后的旗叶內肽酶（EP）活性、旗叶游离氨基酸含量、子粒醇溶蛋白含量、高分子量谷蛋白亚基（HMW-GS）和低分子量谷蛋白亚基（LMW-GS）含量、HMW-GS / LMW-GS比值、子粒蛋白质含量、公顷穗数和穗粒数、子粒产量均显著升高,面团形成时间和稳定时间延长;继续增加施氮量至240 kg/hm2,GS活性无显著变化,但开花21 d后的EP活性、-醇溶蛋白、-醇溶蛋白、HMW-GS、LMW-GS和子粒蛋白质含量仍显著提高,面团稳定时间继续延长,子粒产量显著降低。说明施氮过多对小麦氮素同化和产量无益;提高开花后旗叶GS活性和灌浆后期旗叶EP活性,有利于HMW-GS和LMW-GS的积累及HMW-GS/ LMW-GS比值的提高。适量施氮不仅提高了子粒灌浆所需氮源的供给能力,而且显著增加公顷穗数和穗粒数,扩大了单位面积库容,增加了单位面积上的氮素和光合产物在子粒中的贮存,这是适量施氮实现子粒品质和产量同步提高的生理原因。本试验条件下高产优质高效的施氮量为168～240 kg/hm2。     

施氮量和蚕豆/玉米间作对土壤无机氮时空分布的影响

在田间条件下于2006—2007年研究了不同氮水平下（N 0、75、150、225、300 kg/hm2）蚕豆/玉米间作体系与其相应单作体系土壤无机氮的时空分布规律,旨在为河西走廊灌区蚕豆/玉米间作体系的氮素管理提供理论依据。用土钻法采集土壤剖面样品,CaCl2浸提,流动分析仪测定土壤无机氮的方法研究了施氮量和蚕豆/玉米种间相互作用对土壤无机氮时间和空间变化特点。结果表明:灌漠土无机氮以NO3--N为主。蚕豆和玉米无机氮含量在蚕豆收获前种植方式间均无显著性差异,蚕豆收获后至玉米收获,间作显著降低了两种作物各层无机氮含量;无机氮含量随着施氮量增加而显著增加。蚕豆收获后间作体系0—100 cm土层无机氮累积量略高于单作体系,且0—100 cm 土层无机氮累积量高于100—160 cm土层;玉米收获后,间作蚕豆和玉米土壤无机氮累积量在0—100 cm土层分别平均降低了51.7%和16.6%,在100—160 cm土层平均降低了42.1%和6.1%;与不施氮相比,施氮蚕豆和玉米无机氮累积量在0—100 cm土层分别平均增加了40.1%和81.5%,在100—160 cm土层分别增加了69.6%和40.6%;与单作体系相比,间作体系0—100 和100—160 cm土层土壤无机氮分别降低43.4%和34.1%。因此,施氮肥显著增加土壤无机氮的累积,而豆科/禾本科间作减少了土壤无机氮的残留。     

Yield and yield components of two irrigated red bean cultivars and some soil properties as influenced by wheat residues and nitrogen management

Crop residues are beneficial substances affecting crop production and soil properties. A field experiment was carried out to evaluate the effects of wheat (Triticum aestivum L.) residue rates (0, 25, 50 and 75%) combined with N levels (0, 34.5, 69, 103.5 kg ha^?1) on yield and yield components of two red common bean (Phaseolus vulgaris L.) cultivars and to monitor chemical soil parameters. The experiment was conducted at Research Center, College of Agriculture, Shiraz University, Shiraz, Iran for two years (2008–2009). The experiment was conducted as a split–split plot arranged in a randomized complete blocks design with three replications. The highest seed yield was obtained when 25–50% of residues were incorporated. The highest seed yield, seed weight per plant, 100-seed weight and seed number per pod were obtained with 103.5 kg N ha^?1 with no significant difference to 69 kg N ha^?1. Residue incorporation significantly increased soil organic carbon (SOC) as well as available K and P content. It is possible to sow red common bean as a double cropping by soil incorporation of 25–50% wheat residues with application of 69 kg N ha^?1.     

Effect of phosphorus,nitrogen fertilization and foliar applied manganese on yield and nutrient concentration of soybean

Abstract

A greenhouse experiment was conducted on clay loam soil of pH 7.8 to evaluate the effect of P, N fertilization and foliar applied Mn on yield and nutrient concentration in leaves and seeds of soybean. A significant yield increase was obtained for each added increment of P fertilizer up to 30 kg P₂O₅/acre. At application rates of 0, 15, 30 & 45 kg P₂O₅/acre, the seed yield was subsequently 27.8, 65.8, 82.8 & 83.6% of the maximum yield obtained at the maximum calculated P level. The yield increase accounted 14.2 and 10.2% for N and Mn applications. In all treatments, in which P was combined with N, the seed yield was relatively higher than with P or N applied alone.

Phosphorus, N and Mn content in leaves and seeds were positively correlated with the applied nutrient fertilizers. At any given level of applied P and Mn, P content was significantly higher in +N than ‐N treatments. By Mn application, P content in leaves and seeds was decreased. Phosphorus and Mn applications did not influence N content in leaves and seeds.     

安徽省农田氮磷化肥施用合理性分析

基于化肥施用环境安全阈值模型,提出化肥施用指数的概念,建立了化肥施用合理性评价方法,从时空二维角度对安徽省氮、磷化肥施用的合理性进行分析,为安徽省化肥减量增效提供理论基础和现实指导。研究结果表明:1987~2015年安徽省单位播种面积氮、磷化肥施用量经历了快速增长-波动增长-平稳增长的3个阶段。20世纪80年代末期,安徽省氮肥的施用是合理的,磷肥的施用不足;90年代以后,氮肥施用一直处于过量状态;磷肥施用由合理施用变为过量施用。2015年安徽省16个地市中,有一半属于施氮合理,其余为施氮不足和过量施氮;除5个地市为施磷不足和合理施磷外,其余都为过量施磷。总之,目前安徽省磷肥过量施用情况比氮肥严重,大多数地市以降低磷肥施用量为首要任务;此外,需结合安徽省有机肥的施用情况,进一步评估氮、磷化肥施用合理性。     

Effect of manganese on concentrations of Zn,K, Ca and Mg in two bush bean cultivars grown in solution culture

Two bush bean cultivars [Phaseolus vulgaris L. cv. ‘Wonder Crop 2’ (WC‐2) and ‘Green Lord’ (GL)], differing in Mn toxicity, were grown in a growth chamber for 12 days in Hoagland No. 2 nutrient solution containing 0.05 to 1 ppm Mn as MnCl₂4H₂O with 1 ppm Fe as Fe‐EDTA, at an initial pH 5.00. Concentrations of Zn, K, Ca and Mg in the tissues of two bush bean cultivars were examined in relation to Mn toxicity.

The concentration of Zn in the leaves of Mn‐sensitive WC‐2 increased significantly with increasing Mn concentration in the solution, but such levels were not toxic to the plants.

The percent distribution of Zn and K in Mn‐sensitive WC‐2 plants (% of total uptake) significantly increased in the tops and decreased in the roots with increasing Mn concentration in the nutrient solution; however, Mn treatment had no effect on distribution of either Ca or Mg in WC‐2. External Mn concentration had little or no effect on the K, Ca, or Mg concentration in the tops of Mn‐tolerant GL.     

灌溉、施肥和浅水埋深对小麦产量和硝态氮淋溶损失的影响

利用ArcGIS9.0软件中嵌套地质统计模块分析河套灌区浅层地下水埋深（2009年）空间分布状况,结合在2010年3－7月间开展的灌溉量、施氮量和浅层地下水埋深对春小麦产量和土壤中硝态氮淋溶损失影响的显著性以及最优组合研究,确定出适用于河套灌区内不同区域的春小麦农业管理的最优综合模式。研究表明,表层（0～80 cm）土壤含水率随着浅水埋深的增大而减小,当浅水埋深≥2.0 m时,在同一浅水埋深水平下灌溉量成为土壤含水率显著影响因子;对春小麦产量影响程度高低是浅水埋深＞灌溉量＞施氮量,影响显著因子为浅水埋深;对硝态氮淋溶量影响程度高低是灌溉量＞施氮量＞浅水埋深;灌溉量对硝态氮淋溶影响呈极显著性,施氮量对其影响呈显著性,而浅水埋深起到辅助作用。灌区年均浅层地下水埋深主要有3个阈值：1.25～1.75、1.75～2.25和2.25～3.00 m。在灌区内浅水埋深不同区域内（1.5、2.0和2.5 m）时,三因素最优综合组分别为灌溉量（280 mm）+施肥量（尿素150 kg·hm-2,二铵165 kg/hm2）、灌溉量（320 mm）+施肥量（尿素150 kg/hm2,二铵165 kg/hm2）和灌溉量（360 mm）+施肥量（尿素255 kg/hm2,二铵375 kg/hm2）。     

氮磷钾配施对紫锥菊有效成分含量的影响

研究氮磷钾施肥对紫锥菊有效成分含量的影响。通过液体培养试验,初步分析不同施肥条件下紫锥菊中咖啡酸、绿原酸、单咖啡酰酒石酸和菊苣酸的含量变化。结果表明,N肥对4种有效成分影响各不相同,P肥以不施为宜,高量K肥对紫锥菊植株中4种有效成分的积累都是有利的。不施P肥,450 mg/L的N肥,783mg/L的K肥最有利于植株中主要有效成分菊苣酸的积累。