Characteristics of natural growing areas for Leucojum aestivum L. in Bulgaria based on chemical composition of plants

The characterization Bulgarian natural growing areas of Leucojum aestivum L. was on the basis of the chemical composition of the plants and some soil characteristics which give an opportunity to choose those conditions suitable for growing and obtaining enlargement of the source of plant material with high galanthamine content. This information should benefit the improvement of natural growing areas for Leucojum aestivum L. as well as for the creation of new industrial plantations.     

松嫩平原西部典型盐生景观土壤分形维数及盐碱化特征

为探究松嫩平原西部典型盐生景观土壤颗粒粒度组成及分形特征与土壤盐碱化之间的关系,采用体积分形维数模型计算了6种典型盐生景观土壤表层0—30 cm颗粒组成及分形维数,结合相关分析法分析了土壤分形维数与质地、盐碱特征之间的相关性。结果表明:(1)6种典型盐生景观中农田土壤分形维数值最小(2.35),碱蓬群落土壤最大(2.61),分形维数均值为2.48,分形维数随盐生景观土壤碱化程度增加而增大。(2)土壤质地越粗,砂粒含量越高,分形维数值越小;土壤质地越细,黏粒和粉粒含量越高,分形维数越大。(3)分形维数与盐分含量呈显著正相关(r=0.87,P0.05),尤其与盐分组成中HCO_3~-含量呈极显著正相关(r=0.89,P0.01),与养分中总磷含量呈极显著正相关(r=0.90,P0.01)。(4)土壤颗粒组成中砂粒、粉粒和黏粒与盐分、HCO_3~-及总磷含量也存在极显著相关性。不同盐生景观土壤分形维数与土壤颗粒组成和土壤盐分之间存在显著的相关性,因此可以用分形维数来反映土壤质地和指示土壤盐碱化特征。     

不同细菌饲喂细菌线虫对土壤细菌数量、活性以及群落结构的影响

The effects of bacterial-feeding nematodes on bacterial number, activity, and community composition were studied through a microcosm experiment using sterilized soil inoculated with soil bacteria （soil suspension） and with bacteria and three species of bacterial-feeding nematodes （ Cephalobus persegnis, Protorhabditis filiformis, and Caenorhabditis elegans）. Catalyzed reporter deposition-fluorescence in situ hybridization, CO2 evolution, and denaturing gradient gel electrophoresis （DGGE） of PCR ampli- fied 16S rRNA gene fragments were used to investigate bacterial numbers, antivity, and community composition, respectively. Our results showed that bacterial numbers and activity significantly increased in the presence of bacterial-feeding nematodes, which indicated that bacterial-feeding nematodes had a significant positive effect on soil bacteria. The different nematode species had different effects on bacterial numbers and activity. C. persegnis and P. filiformis, isolated from native soil, increased the bacterial number and activity more than C. elegans. The DGGE analysis results showed that dominant bacterial species significantly differed among the treatments, which suggested that bacterial-feeding nematode species modified the bacterial community composition in soil. Further gene sequence analysis results showed that the dominant bacterial species in this study were gram-negative bacteria. Given the completely same conditions except nematode species, the varied selective feeding behavior of different nematode species was the most likely reason for the altered bacterial community composition. Overall, the alteration of bacterial numbers, activity and community composition resulting from the bacterial-feeding nematodes may ult!mately affect soil ecological functioning and processes.     

Effect of fertilizers on galanthamine and metabolite profiles in Narcissus bulbs by 1H NMR

Narcissus bulbs contain the biologically active alkaloid galanthamine, and Narcissus is being developed as a natural source of the molecule for the pharmaceutical industry. The effect of fertilizer on galanthamine production was investigated in a field study using a (1)H nuclear magnetic resonance (NMR) metabolite profiling approach. Galanthamine was quantitated and major metabolites in the bulbs were identified. The application of standard fertilization levels of nitrogen and potassium caused a significant increase in galanthamine as compared to a control. Multivariate data analysis of the (1)H NMR data revealed that applying double the standard level of nitrogen fertilizer resulted in production of more amino acids and citric acid cycle intermediates, but not more galanthamine. The results indicated that standard levels of fertilizer currently applied in The Netherlands are sufficient for optimal galanthamine accumulation in the bulbs. This study shows how (1)H NMR-based metabolic profiling can provide insight into the response of plant metabolism to agricultural practices.     

Modelling the concentration or dilution of saline soil-water systems

For the survey of saline and alkali soils sampling soil solution in situ is difficult. An alternative is to take soil samples, analyse their extracts and then calculate the chemical composition of the soil solution. A model (EXPRESO) has been designed to calculate both the chemical speciation of electrolyte solution and the exchange equilibrium with an adsorbed phase during simulated dilution and concentration. EXPRESO was validated using reclaimed saline soil samples. Results demonstrated that EXPRESO is a thermodynamically coherent and mass conservative model that allows the calculation of the chemical composition of both the soil solution and the exchange complex over a large range of soil water content (0.250–5 kg water kg^–1 soil). EXPRESO enables the estimation of the chemical composition of the in situ soil solution, giving a comprehensive picture of the soil status under different field conditions. Modélisation de la concentration ou de la dilution des systèmes eau-sols salés Résumé     

西南干暖河谷土壤细沟可蚀性垂直地带性差异

为探究以干暖河谷为基带的西南山地土壤细沟可蚀性垂直地带性差异,选择该区不同垂直带典型土地利用类型(林草地和耕地)下不同土壤类型(红壤、黄壤、棕壤和暗棕壤)为研究对象,利用室内“V”形细沟模拟装置,分别进行6种流量(150,300,450,600,750,900mL/min)和3种水文状态(排水状态、饱和状态和渗流状态)的模拟冲刷试验。结果表明:(1)该区土壤颗粒组成无明显的垂直地带性,林草地的砂粒含量偏高,各土壤的颗粒含量差异在9%以内,所有土壤均为粉质壤土,但有机质差异较大,且随着海拔增加而增加;(2)土壤的细沟可蚀性具有明显的垂直地带性,随海拔升高,林草地土壤可蚀性Kr值逐渐减小,相比于红壤,暗棕壤的Kr值减小幅度平均为47.74%,耕地土壤中黄壤略大于红壤,可能与人为活动强度和土壤熟化程度有关;(3)不同土地利用方式下,林草地土壤Kr值显著大于耕地土壤Kr值,平均增幅为22.63%,这可能与有机质促进土壤抗侵蚀性能的提高有关;(4)不同近地表水文状态下,土壤的细沟可蚀性表现为渗流状态>饱和状态>排水状态,表明随着近地表水文状态变化,尤其是垂直水力梯度的增加会显著导致土壤的细沟可蚀性增大;试验还发现,土壤的临界剪切力仅在不同的水文状态下具有渗流状态>饱和状态>排水状态的规律性,而在不同海拔和土地利用类型之间没有明显的规律性。     

设施种植模式对土壤细菌多样性及群落结构的影响

为了研究有机和常规设施种植模式及轮作对土壤细菌多样性和群落结构的影响,本研究采用Illumina平台Hiseq 2500高通量测序技术,于2016年6月(作物处于收获期)对北京市顺义区不同设施种植模式(分别为有机设施种植模式和常规设施种植模式下的叶菜连作、茄果连作和叶茄轮作)下土壤细菌进行16S r RNA测序。测序质控后共获得17 278个操作分类单元(operational taxonomic units,OTUs),共计318 851条有效序列。比较不同种植模式和轮作下土壤细菌多样性、细菌群落结构组成、相对丰度及土壤理化性质与细菌群落多样性关系的差异性。结果表明:土壤微生物群落结构在有机和常规设施种植模式下差异明显,有机设施种植土壤细菌多样性高于常规设施种植;有机设施种植下轮作与连作土壤细菌群落结构表现出明显差异,而常规设施种植下,两者没有明显差异;有机种植模式下,轮作土壤细菌群落多样性高于连作土壤;设施种植土壤细菌群落主要属于鞘氨醇单胞菌属(Sphingomonas,5.05%)和芽孢杆菌属(Bacillus,4.84%),相对丰度大于0.5%的共有14个属。有机设施种植土壤含有较多促进植物生长、有机质分解的细菌,常规设施种植土壤中降解化学杀虫剂、防治土壤病害、促进硝化过程的细菌较多。RDA分析结果显示土壤细菌群落主要受全磷、速效磷、有机质的影响。Tumebacillus、Candidatus Solibacter和Acidothermus都是分解有机质、利用碳源的细菌属,与土壤有机质含量呈正相关关系。由此可见,设施条件下,有机和常规种植土壤微生物群落结构的差异性主要源于肥料使用、有害生物防治措施和管理方式的不同。有机设施种植模式下,轮作更有利于发挥其改良土壤营养循环和防治土壤病虫害的作用。上述结果为在微生物水平上研究设施条件下不同种植模式的土壤生态质量差异提供了参考。     

Foliage application of nitrogen has less influence on soil microbial biomass and community composition than soil application of nitrogen

Purpose

Many studies have shown the simulated effects of nitrogen (N) deposition on soil microbial community composition by adding N directly to the forest floor but have ignored the N retention process by the canopy. This study was conducted to compare the responses of soil microbial biomass and community composition between soil application of N (SAN) and foliage application of N (FAN).

Materials and methods

A pot experiment was designed with (1) two N application methods (SAN and FAN), (2) three N application levels (5.6, 15.6 and 20.6 g N m^?2 year^?1), and (3) two tree species (Schima superba Gardn. et Champ. and Pinus massoniana Lamb.) following a nested factorial design. Soil microbial biomass and community composition were determined using phospholipid fatty acids (PLFAs) techniques after 1 and 1.5 years of treatments.

Results and discussion

Nitrogen addition increased (P?<?0.05) soil NH₄⁺-N content and soil NO₃^?-N content and decreased (P?<?0.05) soil pH and soil microbial (bacterial, fungal, and actinomycete) biomass for both N application methods. Compared with the SAN treatment, the FAN treatment had higher (P?<?0.05) pH and lower (P?<?0.05) contents of soil NH₄⁺-N and soil NO₃^?-N. Soil microbial biomass and community composition were significantly different between the different N addition levels under the SAN treatment, but they showed no significant difference (P?<?0.05) between the different N addition levels under the FAN treatment. The soil microbial biomass in the S. superba soil was higher (P?<?0.05) than that in the P. massoniana soil for the FAN treatment, with the opposite trend observed under the SAN treatment. Moreover, redundancy analysis showed that soil microorganisms were significantly correlated with soil pH, soil water content, NH₄⁺-N, and NO₃^?-N.

Conclusions

The results showed that N addition affected soil properties, microbial biomass, and the composition of microbial communities; however, the FAN treatment had less influence on soil properties and soil microorganisms than did the SAN treatment over short time scales, and the extent of this effect was different between coniferous and broadleaf trees.

     

Functional resilience of soil microbial communities depends on both soil structure and microbial community composition

The effects of soil structure and microbial community composition on microbial resistance and resilience to stress were found to be interrelated in a series of experiments. The initial ability of Pseudomonas fluorescens to decompose added plant residues immediately after a copper or heat stress (resistance) depended significantly on which of 26 sterile soils it was inoculated into. Subsequent studies showed that both the resistance and subsequent recovery in the ability of P. fluorescens to decompose added plant residues over 28 days after stress (resilience) varied significantly between a sandy and a clay-loam soil. Sterile, sandy and clay-loam soil was then inoculated with a complex microbial community extracted from either of the soils. The resulting microbial community structure depended on soil type rather than the source of inoculum, whilst the resistance and resilience of decomposition was similarly governed by the soil and not the inoculum source. Resilience of the clay-loam soil to heat stress did not depend on the water content of the soil at the time of stress, although the physical condition of the soil when decomposition was measured did affect the outcome. We propose that soil functional resilience is governed by the physico-chemical structure of the soil through its effect on microbial community composition and microbial physiology.     

中国长白山北坡土壤中小型土壤动物沿海拔梯度的群落组成及其空间分布

The Changbai Mountains, located in northeastern China, show clear vertical zonation of vegetation types. Six different habitats, namely Pinus koraiensis mixed broad-leaved forest, Pinus koraiensis-Picea forest, spruce-fir forest, Betula ermanii forest, alpine meadow and alpine semi-desert, at elevations ranging from 780 to 2 480 m, covering almost all ecosystems on the north slope of the Changbai Mountains, were investigated to determine: i) whether or not the community composition of soil mesofauna varied significantly at different elevations; ii) if different soil mesofauna groups would respond differently to elevation and iii) which factors influenced the spatial distribution of soil mesofauna along elevation. Soil mesofauna were collected from each habitat in spring (May), summer (July) and autumn (September) of 2009. The soil mesofauna communities were comprised of at least 44 groups and were dominated by Acari and Collembola, followed by Coleoptera, Diptera larvae and Enchytraeidae. The composition, diversity and abundance of soil mesofauna varied among the six habitats. Meanwhile, significant seasonal variations were observed in the composition, abundance and diversity of the soil mesofauna in each habitat. The taxonomic richness and Shannon index were affected by elevation and soil properties, while the abundance was only significantly affected by soil properties. With regard to taxa, the habitats and seasons had significant effects on almost all the abundances of the major taxonomic groups. The abundance of more taxonomic groups was significantly influenced by the soil properties, while those of Geophilomorpha, Araneae and other taxa were affected by elevation. It is concluded that the composition and spatial distribution of the soil mesofauna varied along the elevation gradient on the north slope of the Changbai Mountains, which might be largely related to the variations of the plant community, soil properties and climate change resulting from the elevation gradient.     

土壤线虫群落对过量施用农用化学品的响应

为研究施用过量的农用化学品对土壤线虫群落组成及多样性的影响,采用定点试验的方法,在哈尔滨市呼兰区选择典型农田生态系统进行试验,对比研究土壤线虫群落对施用过量的氮肥、磷肥、钾肥、除草剂及杀虫剂的响应。在试验田中共鉴定出土壤线虫27科45属,其中Cephalobus和Aphelenchus为优势属。施用不同浓度的各类农用化学品对土壤线虫群落组成、多样性均产生一定影响。线虫总数及食细菌线虫、食真菌线虫、植物寄生线虫数量在不同处理间均存在显著差异(P<0.05);其中,植物寄生线虫的相对丰度随化肥施用量的升高呈增加趋势。从土壤线虫的生态指数来看,除PPI(植物寄生线虫成熟指数)外,其他生态指数[MI(成熟度指数)、F/B(食真菌线虫与食细菌线虫数量比值)、Evenness(均匀度指数)、SR(丰富度指数)、H’(多样性指数)]在施用不同农用化学品处理之间也存在显著差异,并且,MI随着施用钾肥、氮肥浓度的增加而降低。土壤线虫可以作为揭示施用农用化学品过程中土壤质量变化的生物学指标,其群落及多样性的变化表明土壤线虫群落对农用化学品的过量施用产生了响应,过量施用农用化学品会增加土壤生态系统的干扰,对土壤环境造成威胁。     

马铃薯||玉米间作对土壤细菌多样性的影响

为探明连续马铃薯、玉米单作及间作种植对土壤细菌群落组成的影响,利用IonS5~(TM)XL高通量测序平台,分析了单作玉米(M)、单作马铃薯(P)、马铃薯||玉米间作(PM)下,土壤细菌群落组成以及多样性间的差异。结果表明:与单作相比,马铃薯||玉米间作土壤有机质含量显著升高(P0.05),但土壤全氮、碱解氮、全磷、速效钾、土壤pH等没有显著变化。所获得的56 787个土壤细菌可操作分类单元(OTUs)共分为46门、55纲、114目、208科、455属。土壤变形菌门(Proteobacteria)、酸杆菌门(Acidobacteria)和放线菌门(Actinobacteria)细菌占总相对丰度的57.68%～65.11%,为优势菌门;间作对土壤细菌群落多样性(香农指数、辛普森指数)、丰富度(ACE指数和Chao1指数)无显著影响,但改变了基于门、属水平上的细菌群落组成。与单作马铃薯相比,间作显著降低了土壤变形菌门(Proteobacteria)相对丰度(P=0.023),提高了浮霉菌门(Planctomycetes)的相对丰度(P=0.043)。在属水平上,相对丰度较低的芽单胞菌属(Gemmatimonas)、Candidatus Solibacter属更易受到种植方式的影响;间作提高了节杆菌属(Arthrobacter)、芽球菌属(Blastococcus)和芽孢杆菌属(Bacillus)的相对丰度。随细菌群落结构变化,细菌群落功能上出现差异,通过KEGG功能预测共得到7个一级功能层, 35个二级功能层,表现出功能上的丰富性,土壤细菌群落在代谢、遗传信息处理和细胞过程方面功能活跃。7个一级功能层中的代谢功能组在马铃薯||玉米间作与马铃薯单作间有显著差异。利用前向选择,经蒙特卡罗检验表明,连续马铃薯、玉米单作及间作栽培5年后的土壤各理化性状指标与土壤细菌群落组成、多样性间的相关性均不显著。连续马铃薯||玉米间作及单作5年条件下土壤细菌群落组成的变化是由马铃薯||玉米间作作物种间互利和竞争关系而驱动的。     

黄土高原沟壑区不同杏树嵌套种植模式对土壤微生物组成的影响

[目的]研究黄土高原地区土壤微生物群落结构及其主要生理类群在杏树不同嵌套种植模式中的地位和作用。[方法]用"平板培养法"和常规方法,研究了不同嵌套模式下土壤微生物三大类群、主要功能群组成、土壤全氮及有机质含量的变化,并以系统聚类及主成分分析(PCA)等方法进行数据分析。[结果](1)土壤微生物总数量及三大类群数量以杏树与紫花苜蓿的嵌套模式最高(p0.05),杏树嵌套黄花菜和紫花苜蓿次之(p0.05),其余嵌套模式间无显著性变化(p0.05);(2)在杏树和紫花苜蓿的模式中土壤固氮菌数量、氨化细菌和纤维素分解菌数量最高(p0.05),杏树和黄花菜和紫花苜蓿嵌套模式次之(p0.05);(3)土壤微生物三大类群中,土壤放线菌数量对Shannon—Wiener指数的大小变化起着较大作用;(4)依据可培养微生物组成,5种嵌套模式可划分为3个类群,嵌套植被对土壤微生物组成的影响不同,紫花苜蓿对土壤微生物学性质的作用更强。[结论]杏树在不同嵌套种植模式下土壤微生物群落结构组成与嵌套植被类型关系密切且具有其独特的规律及变化原因。     

Effects of experimental restoration on the diaspore bank of an upland moor degraded by Pteridium aquilinum invasion

We assessed the effects of a range of experimental restoration treatments on the diaspore bank (seed and spores) on an upland moor infested by dense Pteridium aquilinum, 9 years after implementation. Experimental treatment included both Pteridium control (cutting, spraying and combinations) and restoration treatments (grazing, seeding); both univariate and multivariate analyses of variance were used to assess significant effects. The seed bank was made up largely of Calluna vulgaris, Juncus effusus and Agrostis capillaris with the majority germinating from the litter (38 per cent) and upper soil layer (53 per cent). Soil depth influenced species composition and density, with greatest numbers near the surface, in keeping with a negative exponential response. In the lowest soil depth only ferns were abundant. A. capillaris seed density was greater in the most disturbed treatment (cut twice yearly with grazing), whereas C. vulgaris was greater in the low‐disturbance treatment (herbicide application only). The diaspore bank composition response identified that certain treatment combinations had a grass‐heath composition in the upper soil layer, which may assist future restoration. There was a significant effect of C. vulgaris seed addition detected in the Pteridium litter layer suggesting that this litter acted as a barrier to seed transfer to the soil. There was little evidence of coupling developing between the diaspore bank and vegetation. The most successful treatment combination was cutting twice per yr with low grazing plus C. vulgaris addition as brash. Where seed is added the Pteridium litter should be disturbed. Copyright © 2007 John Wiley & Sons, Ltd.     

闽南山地3种典型植被类型土壤分形与养分特征

[目的]探索闽南山地不同植被类型土壤分形特征及其与表层土壤颗粒组成、养分含量的相关关系,为评价闽南山地典型植被类型土壤质量,开展土地利用结构调整、土壤侵蚀防治及生态修复等提供参考。[方法]选取土壤颗粒体积分形维数模型对3种典型植被类型林地土壤的分形维数D值进行计算,并与土壤颗粒组成及土壤养分进行相关性分析。[结果]土壤颗粒体积分形维数D值从大到小依次为次生林马尾松林柑橘林;分形维数D值与土壤黏粒和粉粒体积百分数存在显著正相关关系,与砂粒体积百分含量呈显著负相关(p0.05);土壤pH值、全氮、全磷、全钾、水解性氮、有效磷、速效钾和有机碳含量从大到小均为:次生林马尾松林柑橘林;土壤容重为柑橘林马尾松林次生林;分形维数D值与柑橘林的土壤水解性氮含量呈显著正相关,与马尾松林的水解性氮含量、土壤全磷、全钾含量呈显著正相关,与次生林的土壤全磷含量、水解性氮含量呈显著正相关,与有机碳含量均呈显著负相关。[结论]闽南山地不同植被类型会显著影响表层土壤体积分形维数及养分含量,利用土壤体积分形维数可一定程度上反映不同植被类型的土壤颗粒组成及其养分含量。     

砒砂岩区不同退耕还林措施土壤颗粒及交换性能分布特征

为研究砒砂岩区退耕还林还草措施下林地和草地的土壤结构及土壤交换性能对其措施的响应,选取柠条林、油松林、小叶杨林和本氏针茅草地为研究对象,并以荞麦坡耕地为对照,通过野外取样与室内试验相结合的方式,采用分形理论探究土壤粒径分布(PSD)、阳离子交换量(CEC)和交换性盐基总量(ECEC)及其组成(Na~+、K~+、Ca~(2+)、Mg~(2+))的分布特征,并分析其相关性关系。结果表明:(1)实施退耕还林措施后,草地和林地的PSD分布范围、非均一性、离散程度均高于坡耕地,且柠条林的土壤粉粒含量、PSD分布范围的增幅效果最显著(P0.05);土壤剖面垂直层次上,草地有利于增加表层土壤的细粒组分和粒径的分布范围,而林地更有利于对深层土壤粒径的改良与细化;(2)研究区交换性盐基组成主要以碱土金属为主(Ca~(2+)、Mg~(2+)),不同措施的土壤ECEC和CEC值由大到小依次为柠条林油松林小叶杨林草地坡耕地。草地表层土壤交换性能优于底层土壤,而林地与之相反;(3)黏、粉粒和细砂是决定研究区土壤交换性能的细粒土壤和粗粒土壤,粉粒是CEC、ECEC的主要贡献因子,多重分形维数可较好地描述土壤交换性能与土壤颗粒间的关系。不同措施以柠条林对土壤颗粒组成和土壤交换性能的改良效果最优。     

羟基磷灰石–植物联合修复对Cu/Cd污染植物根际 土壤微生物群落的影响

针对江西贵溪Cu、Cd重金属污染土壤,通过田间试验,比较无机生物材料羟基磷灰石及3种植物(海州香薷、巨菌草、伴矿景天)与羟基磷灰石联合修复对土壤总Cu、Cd的吸收及对活性Cu、Cd的钝化吸收能力差异。采用磷脂脂肪酸(PLFA)分析法,比较不同修复模式对土壤微生物群落结构的影响,以评估土壤微生态环境对不同修复措施的响应。研究结果表明:羟基磷灰石的施加可显著提高土壤pH,并有效钝化土壤活性Cu、Cd含量,但对土壤总Cu、Cd的含量影响较小。植物与羟基磷灰石的联合修复在显著降低土壤活性Cu、Cd(P0.05)的同时,减少了植物根际土壤总Cu、Cd的含量(P0.05)。不同修复措施对土壤微生物群落组成影响差异明显。单独施加羟基磷灰石与土壤真菌群落呈显著正相关,使土壤真菌生物量提高,从而引起真菌/细菌(F/B)的升高。植物与羟基磷灰石的联合修复可有效缓解土壤真菌化的趋势,其中巨菌草与羟基磷灰石的联合修复可有效提高土壤革兰氏阳性、革兰氏阴性细菌生物量及多样性,降低F/B值,从而降低土壤真菌病害的风险。不同植物根系活性代谢引起有机质的积累促进植物与羟基磷灰石处理中根际有机碳含量显著提高。聚类增强树(Aggregated boosted tree,ABT)分析结果表明:不同修复模式是影响土壤微生物群落的重要因素,其次土壤pH和Cu的含量及活性也是改变重金属污染区域微生物群落的因子。该研究从微生物群落结构角度解释了植物与羟基磷灰石联合修复对土壤微生态体系的作用,为开展Cu、Cd等重金属污染地植物与无机生物材料的联合修复方式的筛选及实施提供可靠的理论依据。     

Analysis of the variable charge of two organic soils by means of the NICA-Donnan model

We have tested to see if the generic set of NICA‐Donnan model parameters, used to describe isolated humic substances, can also describe soil humic substances in situ. A potentiometric back‐titration technique was used to determine the variable surface charge of two organic peat soils at three different ionic strengths. The non‐ideal, competitive‐adsorption NICA‐Donnan model was used to simulate the surface charge, by assuming a bimodal distribution of H⁺ affinity on the soil solid phase. The model provided an excellent fit to the experimental data. The Donnan volume, V_D, varied slightly with ionic strength, although the variation was less than for humic substances in solution. The values obtained for the parameters that define the affinity distributions, the intrinsic proton binding constant (log K_i^int) and the heterogeneity of the site (m_i), were similar to those observed for isolated soil humic acids. The abundance of carboxylic groups in the whole soil represented 30% of the typical value for isolated soil humic acids. The composition of the organic matter of the whole soils, obtained by ¹³C CPMAS NMR, was comparable to the characteristic composition of soil humic acids.     

Fungal endophyte Phomopsis liquidambari B3 enriches the diversity of nodular culturable endophytic bacteria associated with continuous cropping of peanut

Previously, the application of the fungal endophyte Phomopsis liquidambari B3 exhibited notable effect in alleviating soil obstacles caused by continuous cropping of peanut. It was supposed that P. liquidambari B3 could enhance the efficiency of nodulation and nitrogen fixation by enriching the diversity of nodular endophytic bacteria under continous monocropping system. To verify this hypothesis, a pot experiment was conducted under natural conditions. Four treatments were set as follow: discontinuous cropping soil (NS), continuous cropping soil (CK), continuous cropping soil inoculate with actived P. liquidambari B3 (CSB3), and continuous cropping soil inoculate with sterilized P. liquidambari B3 (CSIB3). As a result, a total of 120 isolates were obtained. Our study clearly declared that the addition of fungal endophyte effectively enriched the genetic diversity and the community composition in CSB3. In contrast, a low genetic diversity level and simplex community structure were exhibited in CK and CSIB3. Meanwhile, the positive percentage rate and corresponding community composition of the plant growth-promoting isolates in NS and CSB3 were larger and more abundant than the other. This is the first time to describe the effects of fungal endophytes on the diversity of the nodular culturable endophytic bacteria associated with continuous cropping of peanut.     

Phosphorus Composition and Phosphatase Activities in Soils Affected by Long-Term Application of Pig Manure and Inorganic Fertilizers

The long-term (25 years) effect of using chemical fertilizers and animal manure on soil phosphorus (P) composition and phosphatase activities was investigated in this study. Results showed that pig manure applications significantly increased soil total P, Olsen P, and phosphatase activities, whereas chemical fertilizers had no significant effects on soil chemical properties and phosphatase activities. Manure applications doubled or tripled the orthophosphate concentrations as compared to chemical fertilizers. Analysis of solution ³¹P nuclear magnetic resonance (NMR) spectroscopy showed that P composition in sodium hydroxide (NaOH)–ethylenediamenetetraacetic acid (EDTA) extracts was dominated by orthophosphate (59–84%), followed by phosphomonoesters (15–40%). More organic P (P_o), especially myo-inositol hexakisphosphate, was observed in soil treated with manure as compared with soil treated with chemical fertilizer.