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1.
Whether the extent of dry weight inhibition by nitrogen (N) or phosphorus (P) deficiencies on different plant parts is the same and whether imposing moderate N and P deficiencies selectively suppress undesirable vegetative growth has not been studied in Pima cotton (Gossypium barbadense L.). The purpose of this study was to determine the extent to which dry matter accumulation in leaves, stems, and reproductive structures is inhibited by N and P deficiencies in Pima cotton. The study was conducted in 1991 and 1992 in a Uvalde silty clay loam soil (fine‐silty, mixed, hyperthermic Aridic Calciustolls). The treatments included applied rates of 0, 67, 135, 202, and 269 kg N ha‐1 in a factorial combination with 0, 15, 29, and 44 kg P ha‐1. Nitrogen deficiency (0 kg N ha‐1) significantly (P≤0.05) reduced leaf (LDW) and stem (SDW) dry weights in both years and reproductive dry weight (RDW) in 1992. Nitrogen deficiency suppressed dry weight accumulation in leaves to a greater extent than in stems. Relative to 269 kg N ha‐1, the 0 kg N ha‐1 treatment resulted in a maximum LDW reduction of 62% at 144 DAP (days after planting) in 1991 and 36% at 121 DAP in 1992, compared with a corresponding SDW reduction of only 39% in 1991 and 25% in 1992. Dry weight accumulation in reproductive parts was the least affected by N deficiency. The decline in LDW associated with senescence and defoliation began earlier in treatments that received 0 or 67 kg N ha‐1 than treatments that received ≥135 kg N ha‐1. Phosphorus affected LDW and SDW in 1991, but its differential effect on LDW, SDW, and RDW was much smaller than that of N. Imposing a moderate level of N deficiency, not P deficiency, may be an effective Pima cotton management strategy to selectively suppress undesirable vegetative growth and enhance maturity.  相似文献   

2.
A study was undertaken to evaluate the yield, fruit size, and vegetative growth of three strawberry cultivars inoculated with three vesicular‐arbuscular mycorrhizal (VAM) species at three phosphorus (P) fertility levels. Vesicular‐arbuscular mycorrhiza inoculation and P fertility had no effect on inflorescence or flower number, total yield, fruit weight, or crown number. Higher levels of P did not increased total dry shoot weight, total fresh shoot, weight leaf area, total dry root weight, and leaf number in the present of VAM. However, the cultivars responded differently to VAM inoculation. Vesicular‐ arbuscular mycorrhiza inoculation in combination with P at all levels increased total dry and fresh shoot weight, leaf area, and leaf number compared to application of P alone. The results indicated that it may be possible to increase strawberry stolon production by inoculating the strawberry plants with VAM, a technique which might be useful in nurseries to produce certified strawberry plants.  相似文献   

3.
The partitioning of biomass between aboveground parts and roots, and between vegetative and reproductive plant parts plays a major role in determining the ability of cotton (Gossypium hirsutum L.) to produce a crop in a given environment. We evaluated the single and combined effects of water and N supply on the partitioning of biomass in cotton plants exposed to two N supply levels, 0 and 12 mM of N, and two water regimes, well irrigated and water‐stressed at an early reproductive stage. The N treatments began when the third true leaf was visible, while water deficit treatments were imposed over the N treatments when the plants were transferred into controlled‐environment chambers at a leaf area near 0.05 m2. Both water deficits and N deficits inhibited total biomass accumulation and its partitioning in cotton. Water deficit alone and N deficit alone inhibited the growth of leaves, petioles, and branches, but did not inhibit growth of the stem and enhanced the accumulation of biomass in squares. When water deficit was superimposed on N deficit, leaf growth was inhibited, although to a lesser extent than when it was the sole stress factor, and the accumulation of biomass in squares was also inhibited. Yet, the dry weight of squares in plants exposed to water and N deficits was greater than that of non‐stressed plants. Water and N deficits, either alone or in combination, did not inhibit the growth of the tap root. Growth of lateral roots was not inhibited either by water deficit alone or in combination with N deficit, but was enhanced when plants were exposed to N deficit alone. Exposure to water deficit alone or in combination with N deficit decreased the shoot:root ratio through the inhibition of shoot growth. Exposure to N deficit alone decreased the shoot:root ratio through the combination of shoot growth inhibition and root growth enhancement.  相似文献   

4.
Rice grown on the organic soils of the Everglades is routinely fertilized with silicon (Si). The objective of this research was to investigate changes in nitrogen (N) and phosphorus (P) concentration in various plant parts in response to Si fertilization. Two cultivars were grown in lysimeters filled with low‐Si soil. Half the lysimeters were fertilized with calcium silicate to provide 2Mg Si ha‐1 and the other lysimeters remained unfertilized as a control. Nitrogen concentration decreased in all plant parts with Si fertilization. Phosphorus concentration increased with Si. Maturity was earlier in the Si fertilized rice.  相似文献   

5.
Abstract

Boron (B) toxicity in crops occurs in many dry areas. However, the effect of high external B levels on straw quality is not known. In this greenhouse study, the responses of straw yield, straw quality, and other characters to high soil B levels were investigated in barley. Two cultivars, Galleon and Harmal, were tested. In Experiment I, three B levels were prepared by mixing 0, 25, and 50 mg B kg‐1 soil‐mix (designated as B0, B25, and B50, respectively). Straw yield, straw quality (nutritional value), and other characters were measured. In Experiment II, an additional soil B level, prepared by adding 12.5 mg B kg‐1 soil‐mix (designated as B12), was used. Shoots and roots were harvested at tillering and after heading. In Experiment 1, the B50 treatment improved straw quality, had no effect on straw yield, but decreased grain yield, and harvest index. Considering the shoot B concentrations and B uptake at the tillering stage at moderate to high levels of soil B in both experiments, Galleon was able to restrict B uptake better than Harmal. However, Harmal, unlike Galleon, had a better growth and more tillers at moderate levels of soil‐B than at BO. Since Harmal had higher or similar B concentrations as Galleon, Harmal appeared to tolerate higher tissue B concentrations than Galleon. The limitation of selecting genotypes tolerant to high‐B soil was pointed out, and the need to study adult performance after the initial selection was suggested. The finding on straw quality and yield would be a positive finding for sheep owners in dry areas, where barley straw and stubble are a valuable ruminant feed.  相似文献   

6.
Aubergine plants (Solanum melongena cv. Bonica) were grown under controlled greenhouse conditions on a soil substrate supplied with organic fertilizers (15 kg/m2) mixed with calcium sulfate (CaSO4 at 500 g/m2), with different doses of nitrogen (N as N1 = 15, N2 = 22.5, N3 = 30 g/m2) in the form of ammonium nitrate (NH4NO3), and phosphorus (P as P1 = 24, P2 = 36 g/m2) as phosphorus acid H3PO4). Plants were sampled every 15 days, and the pigments chlorophyll a, chlorophyll b, total (a+b) and ratio (a/b), carotene, licopene, and anthocyanins were determined in the leaves. The results showed that increases in rhizosphere N led to increases in foliar concentrations of chlorophyll a and b, both individually and as total chlorophyll, independently of the dose of P applied. Total chlorophyll concentrations were directly correlated with the level of P fertilization. Carotene and licopenes reflected the influence of increasing doses of N, whereas P did not affect these pigments. Anthocyanin levels were affected by both N and P.  相似文献   

7.
Nitrogen (N) supply increased yield, leaf % N at 10 days after silking (DAS) and at harvesting, the contents of ribulose‐1,5‐bisphosphate carboxylase (RUBISCO) and soluble protein, and the activities of phosphoenolpyruvate carboxylase (PEPC), and ferredoxin‐glutamate synthase (Fd‐GOGAT), but not of glutamine synthetase (GS) for six tropical maize (Zea mays L) cultivars. Compared to plants fertilized with 10 kg N/ha, plants inoculated with a mixture of Azospirillum sp. (strains Sp 82, Sp 242, and Sp Eng‐501) had increased grain % protein, and leaf % N at 10 DAS and at harvest, but not grain yield. Compared to plants fertilized with either 60 or 180 kg N/ha, Azospirillum‐inoculated plants yielded significantly less, and except for GS activity, which was not influenced by N supply, had lower values for leaf % N at 10 DAS and at harvest, for contents of soluble protein and RUBISCO, and for the activities of PEPC and Fd‐GOGAT. Yield was positively correlated to leaf % N both at 10 DAS and at harvest, to the contents of soluble protein and RUBISCO, and to the activities of PEPC and Fd‐GOGAT, but not of GS, when RUBISCO contents and enzyme activities were calculated per g fresh weight/min. However, when enzyme contents and enzyme activities were expressed per mg soluble protein/min, yield was correlated positively to RUBISCO and PEPC, but negatively to GS. These results give support to the hypothesis that RUBISCO, Fd‐GOGAT, and PEPC may be used as biochemical markers for the development of genotypes with enhanced photosynthetic capacity and yield potential.  相似文献   

8.
Crop and native plants can be characterized as high and low nutrient‐adapted based on their expected response to native and applied nutrients. Our objective was to compare the plasticity of biomass allocation and tissue nutrient concentrations to added sulfur (S) and nitrogen (N) across a continuum of high and low nutrient‐adapted grasses, represented by barley (Hordeum vulgare), smooth brome (Bromus inermis), bluebunch wheatgrass (Pseudoroegneria spicata), and Idaho fescue (Festuca idahoensis). In our greenhouse study, treatments included two S sources (pyrite and gypsum), at 150 and 300 kg S ha‐1, N at 50 kg ha‐1, and a check. Shoot biomass of barley, smooth brome, and bluebunch wheatgrass was enhanced by S plus N. Shoot biomass of barley and smooth brome was greater with pyrite than with gypsum. Root biomass of smooth brome and bluebunch wheatgrass was greater with pyrite than with gypsum. Plant S concentrations of barley and Idaho fescue were enhanced by added S. Plant S concentrations in barley and smooth brome were greater with gypsum than with pyrite. Except for barley, plant S pools (shoot biomass x shoot S concentration) were enhanced with S plus N compared with no added nutrients. Nitrogen pools of barley, smooth brome, and bluebunch wheatgrass were higher with pyrite than with gypsum. Soil sulfate (SO4) was greater when S or S plus N was added than without any added nutrients. For barley and smooth brome, soil sulfate tended to be lower with pyrite than with gypsum. For all soils, pH was lower with added S or added S plus N compared with unamended soils. While pyrite lowered soil pH, gypsum tended to increase soil pH. Overall, barley and smooth brome were highly plastic in responding to enhanced nutrient levels, bluebunch wheatgrass was relatively responsive, and Idaho fescue was least responsive.  相似文献   

9.
Abstract

Determination of soil aluminum (Al), ammonium‐nitrogen (NH4‐N), and nitrate‐nitrogen (NO3‐N) is often needed from the same soil samples for lime and fertilizer recommendations, but Al has to be extracted and quantified separately from NH4‐N and NO3‐N according to present methods. The objective of this study was to develop a reliable method for simultaneous analyses of soil Al, NH4‐N and NO3‐N using a Flow Injection Autoanalyzer. Thirty‐five soil samples from different locations with wide ranges of extractable Al, NH4‐N and NO3‐N were selected for this study. Aluminum, NH4‐N and NO3‐N were extracted by both 1 M and 2 M potassium chloride (KCl), and quantified using a LACHAT Flow Injection Autoanalyzer simultaneously and separately. One molar KCl was found to be a suitable extractant for all three compounds when compared to 2 M KCl. The 1 M KCl extract proposed could aid in decreasing the costs associated with simultaneous NH4‐N, NO3‐N, and Al analyses. Results of those three compounds analyzed simultaneously were not statistically different from those analyzed separately in 1 M KCl solution. This new procedure of simultaneous determination of NH4‐N, NO3‐N, and Al increases efficiency and reduces cost for soil test laboratories and laboratory users.  相似文献   

10.
Abstract

Fertilizer placement for corn (Zea mays L.) has been a major concern for no‐tillage production systems. This 3‐yr study (1994 to 1996) evaluated fertilizer phosphorus (P) or potassium (K) rates and placement for no‐tillage corn on farmers’ fields. There were two sites for each experiment involving fertilizer P or K. Treatments consisted ofthe following fertilizer rates: 0,19,and 39 kg P ha‐1 or 0, 51, and 102 kg K ha‐I. The fertilizer was broadcast or added as a subsurface band 5 cm beside and 5 cm below the seed at planting. Early plant growth, nutrient concentrations, and grain yields were measured. At the initiation of the study, soil test levels for P and K at the 0–1 5 cm depths ranged from optimum (medium) to very high across sites. Effects of added fertilizer and placement on early plant growth and nutrient concentrations were inconsistent. Added fertilizer had a significant effect on grain yields in two of twelve site‐years. Therefore, on no‐tillage soils with high fertility, nutrient addition, and placement affected early plant growth and nutrient utilization, but had limited effect on grain yield. Consequently, crop responses to the additions of single element P or K fertilizers under no‐tillage practices and high testing soils may not result in grain yield advantages for corn producers in the Northern cornbelt regardless of placement method.  相似文献   

11.
Abstract

An upland rice variety IAC‐47 was grown in a greenhouse to determine the effect of foliar nitrogen (N) supplementation during grain development on the activity of the N assimilation enzymes, nitrate reductase (NR) and glutamine synthetase (GS), on free amino‐N content and leaf soluble sugars, and on grain crude protein content. At 10 and 20 days after anthesis (DAA), the leaves were fertilized with a liquid fertilizer containing 32% N as 12.8% urea, 9.6% ammonium (NH4), and 9.6% nitrate (NO3) in increasing rates corresponding to 0,20+20, 40+40, and 60+60 kg N ha‐1. Leaves were collected twice (at 12 DAA and 14 DAA for GS activity, sugar and amino‐N content, and at 11 and 13 DAA for NRA) after each application of leaf N. The late foliar application of N increased significantly grain crude protein without a corresponding decrease in grain weight. The NR activity (NRA) increased after the foliar application of N. In the flag leaf, 60+60 kg N ha‐1 (21 DAA) resulted in higher NRA (20x over the control), while GS activity was smaller than the control. At 22 DAA there was an increase in GS activity in the flag leaf at 20+20 N level. However, the GS activity decreased as applied N levels increased. Also at the 20+20 level, there were increases in free amino‐N in the flag leaf and second leaf at the final harvest. Throughout the experiment, plants at the 60+60 N level had the lowest levels of soluble sugars. Increases in crude protein were highest at 40+40 N level (27.9%), followed by 60+60 (18.7%).  相似文献   

12.
Bermudagrass (Cynodon dactylon L.) is a warm season perennial that is well adapted in the southern Great Plains. It is one of the region's most important forage crops used for livestock production, and is commonly grown without legume interseeding. Recent research has investigated ways of improving the quality and quantity of this forage. The objectives of this study were to determine the effect of interseeded legumes and phosphorus (P) fertilizer on bermudagrass pasture forage yield and crude protein content. One experiment was initiated in 1993 in eastern Oklahoma in an established bermudagrass pasture. Red clover (Trifolium pratense L.), ladino clover (Trifolium repens L.), and two varieties of alfalfa (Medicago sativah), ’alfagraze’ and'common’, were interseeded by hand into an established stand of bermudagrass. The effect of P on forage yield and crude protein was evaluated using a 30‐kg P ha‐1 rate applied at establishment versus no applied P. Forage yield was collected three times throughout the growing season each year from 1994 through 1997. When both alfalfa varieties were interseeded into a bermudagrass pasture without applying additional P fertilizer, forage yields for the legume‐grass mixtures decreased below those obtained from the monoculture bermudagrass in the first year of the stand. The alfalfa variety ‘alfagraze’ interseeded into established bermudagrass decreased total forage yield over the entire 4‐yr study. Interseeded red clover and ladino clover increased crude protein of the forage compared with monoculture bermudagrass the first two years of the study, with red clover continuing to increase crude protein in the fourth year. However, when 30 kg P ha‐1 was applied to the bermudagrass prior to establishment of the legumes, no change in yield or protein was observed for both alfalfa varieties’ interseeding treatments versus the unfertilized mixtures. Although forage yield may not be increased, interseeding legumes into established bermudagrass could provide an efficient way to improve pasture crude protein without the use of inorganic fertilizers. However, if alfalfa ('common’ or ‘alfagraze') is interseeded, additional P may need to be applied at legume establishment to prevent possible yield decreases.  相似文献   

13.
More information on the response of newly developed or introduced grain sorghum cultivare to split‐applied nitrogen (N) in semi‐arid rainfed agriculture is needed. Therefore, the influence of four split‐applied N schedules (100/0, 66/34, 50/50, and 34/66) on six American (SC 283, SC 274, SC 669, B 66181, SC 33, and RTam 428), and four West African (CSm 63, 1S 6704c, 1S 7173c, and 1S 7419c) grain sorghum cultivars was evaluated. The split‐applied N significantly increased grain yield and percent protein in grain sorghum over a one‐time application of N. The increase in yield and protein content varied among varieties and schedules of N application. Varieties SC 574, RTam 428, and Csm 63 at split‐applied schedules of 66/34, 50/50, and 34/66, respectively, gave the highest yield over one‐time application of N. Similar differences in percent protein in grain among cultivars due to split‐applied N were observed.  相似文献   

14.
Pre‐ and post‐transplant growth of bedding plants is affected by seedling nutrition. However, there is little information available on how seedling nutrition affects the growth of ornamental bedding plants. In this study, we quantified the effects of nitrogen (N) (8 to 32 mM) and phosphorus (P) and potassium (K) concentration (0.25 to 1 mM) of the seedling fertilizer on pre‐ and post‐transplant growth and nutrient element content of salvia (Salvia splendens F. Sellow ex Roem. & Schult.) and vinca (Catharanthus roseus L.) seedlings. Shoot growth of salvia and vinca increased with increasing concentrations of N in the pre‐transplant fertilizer and these differences lasted until the end of the study at 15 days after transplanting. Pre‐transplant root dry mass of these species was not affected by the N concentration of the fertilizer, but root dry mass at 12 days after transplanting was positively correlated with the N concentration of the pre‐transplant fertilizer. Increasing N concentrations in the seedling fertilizer increased tissue N levels of salvia and decreased tissue K level of vinca at transplanting. Increasing P and K concentrations in the pre‐transplant fertilizer increased tissue P level of salvia and P and K levels of vinca, but had little effect on seedling growth. Leaf area and root dry mass at transplanting decreased slightly with increasing P and K concentration in the fertilizer. There were no lasting effects of pre‐transplant P and K concentration of the fertilizer. These results indicate that salvia and vinca seedlings can benefit from high concentrations of N (up to 32 mM) in the fertilizer, while only low concentrations of P and K (0.25 mM) are needed.  相似文献   

15.
Abstract

Four treatments (no‐tillage plus subsoiling, no‐tillage, conventional tillage plus subsoiling, and conventional tillage) were continuously in place for 6 yr and a second set of no‐tillage plus subsoiling and conventional plus subsoiling treatments were continuously in place for 3 yr to study the long‐term effects of conventional and no‐tillage corn on soil organic matter (OM) and N contents. Soil samples were taken at random between the rows and in the rows to a depth of 60 cm, in 5‐cm increments to a depth of 30 cm, and then in 15‐cm increments from the 30 to 60‐cm depth for OM and N determination. No‐tillage resulted in A3 and 20% more Kjeldahl N than conventional tillage in the 0 to 5‐cm soil depth after 6 and 3 yr, respectively. after 6 yr, the 0 to 5‐cm depth had 36% more OM in no‐tillage treatments than in conventional tillage treatments, and soil from no‐tillage treatments averaged 27% more OM than the conventional tillage plus subsoiling treatment at the 0 to 15‐cm soil depth.  相似文献   

16.
Abstract

The interaction of gamma‐irradiated sewage sludge, nitrogen and phosphorus fertilizers on dry matter production, phosphorus concentration and phosphorus uptake of sorghum was examined. Three crops of sorghum were grown in the greenhouse in 19 1 plastic buckets. The phosphorus and sludge treatments were applied at the initiation of the experiment only. Nitrogen was applied to the corresponding nitrogen treatment pots before each of the three croppings.

There was a significant nitrogen x sewage interaction for dry matter production, phosphorus concentration and phosphorus uptake in each harvest. There was a significant phosphorus sewage sludge interaction in the first harvest for phosphorus uptake. All other possible interactions were not statistically significant. The 67 metric ton/ha sludge rate produced nearly the same yield as the nitrogen treatment. In the first harvest, sludge significantly increased plant phosphorus uptake from the fertilizer phosphorus. The phosphorus uptake in sorghum from 472 and 944 kg P/ha from the sludge treatments was comparable to that from 1299 and 2598 kg P/ha from triple superphosphate.  相似文献   

17.
Abstract

Two soil tests commonly used to characterize the availability of soil phosphorus (P) are Bray P1 (B1) and Mehlich 3 (M3) extradants. The objective of this investigation was to compare M3‐ and B1‐extractable P levels in a Starr clay loam (fine‐loamy mixed thermic Fluventic Dystrochrepts) amended with relatively low to high surface applications of poultry litter (PL). The following eight treatments were applied to the soil for pasture renovation in 1991 and 1992: 1) a control, 2) P application as monocalcium phosphate [Ca(H2PO4)2‐H2O], 3) P application as Ca(H2PO4)2‐H2O and nitrogen (N) application as ammonium nitrate (NH4NO3) and urea, and 4) five levels of poultry litter (PL) based on N content. The five P levels as PL were 56.6, 113.2, 169.8, 226.4, and 283.0 kg ha‐1 in 1991, and 49.2, 98.5, 147.7, 196.9, and 246.1 kg ha‐1 in 1992, respectively. Soil samples were obtained from the 0‐ to 5‐cm layer in the spring of 1992 and 1993 for extractable P determination. Levels of P extracted from all treatments by the M3 and B1 extractants were linearly correlated both years (r2=0.96 in 1991 and r2=0.99 in 1992). However, M3 extracted more P from the PL treatments, whereas B1 extracted more P from the control, P application, and N and P application treatments. Curvilinear relationships were obtained between P uptake and levels of either M3‐ or B1‐extractable P for the PL treatments (r2=0.713 for M3 and 0.663 for B1 in 1991 and r2=0.925 for M3 and 0.933 for B1 in 1992). These close relationships in 1992 between extractable P and uptake of P for the PL‐ treated soils indicate that both the B1 and M3 extractants could be used to evaluate excess P in PL‐amended soils.  相似文献   

18.
Abstract

Acid and dilute‐salt chemical extractants are commonly used to assess and adjust phosphorus (P) availability in soils. Soybean was grown for 45 days under greenhouse conditions on samples of 10 Oxisols treated with variable levels of soluble P to compare critical soil P levels between an anion exchange resin (AER) and the Mehlich‐1 extractant, and to evaluate the influence of the soil P capacity factor (PCF) on the critical soil P levels. Variation among soils in the quantities of extracted P and critical P levels with the AER was less than that with Mehlich‐1. Low correlation values between soil characteristics related to PCF and measurements of AER soil P suggested that the AER method was not as sensitive to variations in soil PCF as Mehlich‐1. Interpretations of AER critical P levels, therefore, do not need auxiliary measures of PCF as with the Mehlich‐1 extractant. The reverse was true, however, for P uptake. Therefore, the criteria used to assess plant response to P (P uptake or dry matter production) in soils with variable PCF will influence the results obtained in correlations between methods used to extract soil P.  相似文献   

19.
Two rates of broiler litter (20 and 40 mt/ha) were compared to recommended rates of inorganic nitrogen (N), phosphorus (P), and potassium (K) in a double cropping system of spring sweet corn (Zea mays L. ‘Silverqueen') and fall broccoli (Brassica oleracea L, ‘Southern Comet')‐ Sweet corn matured one week earlier both years when fertilized with 40 mt/ha of broiler litter compared to commercial fertilizer. The early maturity may be due to improved P nutrition. Similar or higher yields of fall broccoli were produced with broiler litter following sweet corn than with commercial fertilizer.  相似文献   

20.
Previous studies have indicated that under hydroponic conditions, spring wheat (Triticum aestivum) plants produce higher grain yields, more tillers, and increased dry matter when continuously supplied with mixtures of NO3 and NH4 than when supplied with only NO3. The objective of this study was to determine if mixed N needs to be available before or after flowering, or continuously, in order to elicit increases in growth and yield of wheat. During vegetative development, plants of the cultivar ‘Marshal’ were grown in one of two nutrient solutions containing either a 100/0 or 50/50 mixture of NO3 to NH4 and, after flowering, half the plants were switched to the other solution. At physiological maturity, plants were harvested, separated into leaves, stems, roots, and grain and the dry matter and N concentration of each part determined. Yield components and the number of productive tillers were also determined. Availability of mixed N at either growth stage increased grain yield over plants receiving continuous NO3, but the increase was twice as large when the mixture was present during vegetative growth. When the N mixture was available only during vegetative growth the yield increase was similar to that obtained with continuous mixed N. The yield increases obtained with mixed N were the result of enhanced tillering and the production of more total biomass. Although plants receiving a mixed N treatment accumulated more total N than those grown solely with NO3, the greatest increase occurred when mixed N was available during vegetative growth. Because availability of mixed N after flowering increased the N concentration over all NO3 and pre‐flowering mixed N plants, it appears that the additional N accumulation from mixed N needs to be coupled with tiller development in order to enhance grain yields. These results confirm that mixed N nutrition increases yield of wheat and indicate that the most critical growth stage to supply the N mixture to the plant is during vegetative growth.  相似文献   

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