Effect of Calcareous Soil on Photosynthesis Rate,Mineral Nutrition,and Source‐Sink Ratio of Table Grape

Abstract

Vitis vinifera L. cv Aurora grafted on S.O.4 (medium lime‐tolerance) rootstock was grown in pot with a high‐carbonate‐soil and a low‐carbonate‐soil. The aim of the trial was to check soil effect on some physiological features such as leaf chlorophyll (Chl) concentration and gas exchange, whole‐canopy gas exchange, mineral nutrition, dry matter partitioning, and technological grape parameters. Measurements for whole‐canopy gas exchange were taken using a custom‐built, flow‐through whole‐canopy gas exchange system set up to run continuous, automated, and simultaneous net carbon exchange rate (NCER) readings of four canopies. The most significant findings were: (a) high‐carbonate‐soil decreased leaf and whole canopy photosynthesis, grape yield, and total dry matter production; (b) high‐carbonate‐soil increased the distribution share of dry matter in the trunk and roots, as compared to the low‐carbonate‐soil, and decreased the share of dry matter in the clusters; and (c) lime‐stress conditions affected mineral nutrition, especially P and K concentrations, which were depressed in most of the organs.     

Comparative growth of two peach seedling rootstocks under alkaline soil conditions

An almond X peach seed line, ‘Titan’ X Nemaguard (T X NG), which is tolerant to lime‐induced chlorosis, was compared to a susceptible seedling rootstock, Nemared, under alkaline conditions. The tolerant rootstock's growth was not affected by Fe stress, whereas the susceptible rootstock showed chlorosis which corresponded to approximately a 20% chlorophyll loss in the new foliage during the 18‐week stress period, a 62% decrease in shoot dry weight and a 22% decrease in plant height.     

The effect of salinity and iron application on growth and mineral uptake of sunflower (helianthus annuus L.)

In a greenhouse experiment, the effect of salinity and Fe chelate on growth and mineral uptake of sunflower (Helianthus annuus L. c.v. Record) was studied.

Sunflower plants were grown in nutrient solution with four levels of salinity (0, 1.5, 3.0 and 4.5 atm), induced by NaCl and four rates of Fe chelate (0, 0.5, 1.0 and 1.5, ppm Fe) as FeEDDHA. The experiment was a completely randomized design with treatment combinations arranged in a factorial manner with three replications.

Dry matter yield, shoot‐root ratio, leaf area, plant height and transpiration decreased as salinity increased, the effect of salinity being depressed by iron applications. Salinity reduced P, K, Ca and Mg uptake by roots as well as that of N, P, K, Ca, Mg by shoots, while Fe applications increased uptake of these elements in roots and shoots. Both salinity and iron applications increased Cl, Na and Fe uptake by roots and shoots, as expected. In most instances salinity reduced uptake of Fe, Mn and Zn by the plants while iron applications improved uptake of these elements.

The sunflower plant used in this experiment was found to be, at least partly, tolerant to salinity and decreased water availability as well as toxicity of ions. Nutritional disorders were the cause of decreased plant growth by increasing salinity of the nutrient solution. The decreased plant growth and mineral uptake, induced by salinity, were partially offset by increased iron levels in the nutrient solution.     

Changes in total protein profiles of barley cultivars in response to toxic boron concentration

In this study, ten‐day‐old seedlings of barley {Hordeum vulgare L. cultivar Anadolu [boron (B)‐tolerant] and Hamidiye (B‐sensitive)} were used. Boron‐treated plants were grown on H₃BO₃ solution (final concentration of 10 mM) for five days. Control plants received no B treatment during this period. Total protein patterns were obtained by analysis of total protein extract from root and leaf tissues of control and B‐treated plants using two‐dimensional gel electrophoresis followed by silver staining. The protein profile of B‐treated seedlings of each cultivar was compared to the profile of control (no stress treatment) plants of the same cultivar. Silver‐stained gels showed that B stress caused increases or decreases in a number of proteins in root and leaf tissues. Moreover, as a result of B treatment, one newly synthesized protein with relative molecular weight (M_r) of 35.0 kDa was detected in root profile of the tolerant cultivar. This protein failed to show up in root profile of the B‐treated sensitive cultivar. Three proteins were quantitatively increased in B‐treated root profile of both cultivars. Following B treatment, three proteins were increased in root profile of the tolerant cultivar, but were not changed in the sensitive one. In leaf tissues, however, there were remarkable changes in total protein profiles after B treatment, relative to the control. Following B treatment, in leaf tissues, at least seven proteins were increased in amount in tolerant cultivar but were unchanged in the susceptible one. In tolerant and sensitive cultivars, amounts of two proteins were increased in B‐treated plants, relative to control seedlings. In addition, four proteins (M_r:29, 58, 58, and 22 kDa) were unchanged in control and B‐treated seedlings of the tolerant cultivar. In the susceptible cultivar however, among these four proteins, the first one (M_r:29) was very much reduced and the others (M_r: 58, 58, and 22 kDa) were completely lost in B‐treated seedlings. Moreover, following B treatment, a set of high‐molecular‐weight proteins was quantitatively decreased in the susceptible cultivar but was unchanged in the tolerant cultivar. These results indicate that in barley, certain proteins may be involved in tolerance to B toxicity. In this study, changes in polypeptide composition as a result of B toxic concentration in leaf tissues were more abundant than in roots. Therefore, it is suggested that these changes, especially at shoot level may form the basis of the tolerance mechanism to B toxicity.     

Influence of Ca concentration on micronutrient imbalances in in vitro propagated prunus rootstock

In vitro propagated plums of St. Julien GF 655–2 [Prunus insititia (L.)] (655–2), Damas GF 1869 [Prunus domestica (L.)] (D1869), and Clark Hill Redleaf [Prunus salicina (Until.) X Prunus cerasifera (Ehrh.)] (CHR), were grown in the greenhouse in nutrient solutions of 2, 6, 22, 66, 202, and 404 μM Ca for 96 days. 655–2 plants became severely chlorotic in Ca treatments of 66, 202, and 404 μM concentration after 86 days of growth. D1869 plants in 202 and 404 μM Ca exhibited slight interveinal chlorosis of new growth, while CHR exhibited no chlorosis at any Ca concentration. The best tissue nutrient indicator of chlorosis among rootstocks was foliar P/Fe and P/Zn ratios. 655–2 plants absorbed more P at higher Ca concentrations than did the other rootstock, resulting in the highest stem and leaf P/Fe, and P/Zn ratios. CHR plum may provide an easily propagated, chlorosis‐resistant rootstock for use on calcareous soils.     

Mineral composition of peach leaves affected by iron chlorosis

Abstract

The effect of Fe chlorosis on the mineral composition of field grown peach tree leaves was studied in two different areas. No significant differences in total Fe content were found, whereas 2,2’ bipyridyl extractable Fe, K and the K/Ca ratio were significantly affected in both experiments. Phosphorus and the P/Fe ratio were significantly affected only in one experiment.     

Leaf position and genotype differences for mineral element concentrations in sorghum grown on tropical acid soil 1

Mineral element deficiencies and toxicities are common problems associated with sorghum [Sorghum bicolor (L.) Moench] production on acid soils. To better understand some of the mineral element problems and the analysis of plant tissue of sorghum plants grown on acid soils, four sorghum genotypes were grown on an acid Oxisol at Carimagua, Colombia limed with dolomite at 2 and 6 Mg ha^‐1.

Samples for mineral element analyses were obtained from leaves at different positions on the four genotypes. Concentrations of P and Mg were highest in the flag leaf (Leaf No. 1) and decreased as the position on the plant declined from the top of the plant for plants grown at 2 Mg lime ha^‐1. Similar decreases in P, Mg, K, and Zn concentrations occurred in plants grown with 6 Mg lime ha^‐1. Concentrations of Ca, S, Si, Mn, Fe, Cu, and Al increased as leaf position declined from the flag leaf for plants grown at 2 and 6 Mg lime ha^‐1. The higher lime supply enhanced Ca and reduced Mn and Fe concentrations in leaves. Differences in mineral element concentrations for the four genotypes used were fairly extensive. The elements to show the greatest range among genotypes were Al and Si and the elements to show the least range among genotypes were P, K, and S. Care should be used in collecting leaf samples for plant analysis and genotypic differences for accumulation of mineral elements should be considered in interpretation of results.     

Interaction of Different Iron Chelates with an Alkaline and Calcareous Soil: A Complementary Methodology to Evaluate the Performance of Iron Compounds in the Correction of Iron Chlorosis

Abstract

A great number of studies have shown that the stability of iron chelates as a function of pH is not the unique parameter that must be considered in order to evaluate the potential effectiveness of Fe‐chelates to correct iron chlorosis in plants cultivated in alkaline and calcareous soils. In fact, other factors, such as soil sorption on soil components or the competition among Fe and other metallic cations for the chelating agent in soil solution, have a considerable influence on the capacity of iron chelates to maintain iron in soil solution available to plants. In this context, the aim of this work is to study the variation in concentration of the main iron chelates employed by farmers under field conditions—Fe‐EDDHA (HA), Fe‐EDDHMA (MA), Fe‐EDDHSA (SA), Fe‐EDDCHA (CA), Fe‐EDTA (EDTA), and Fe‐DTPA (DTPA)—in the soil solution of a calcareous soil over time. To this end, soil incubations were carried out using a soil:Fe solution ratio corresponding to soil field capacity, at a temperature of 23°C. The soil used in the experiments was a calcareous soil with a very low organic matter content. The variation in concentration of Fe and Fe‐chelates in soil solution over time were obtained by measuring the evolution in soil solution of both the concentration of total Fe (measured by AAS), and the concentration of the ortho‐ortho isomers for Fe‐EDDHA and analogs or chelated Fe for Fe‐EDTA and Fe‐DTPA (measured by HPLC). The following chelate samples were used: a HA standard prepared in the laboratory and samples of HA, MA, SA, CA, Fe‐EDTA, and Fe‐DTPA obtained from commercial formulations present in the market. The percentage of iron chelated as ortho‐ortho isomers for HAs was: HA standard (100%); HA (51.78%); MA (60.06%); SA (22.50%); and CA (27.28%). In the case of Fe‐EDTA and Fe‐DTPA the percentages of chelated iron were 96.09 and 99.12, respectively. Results show that it is possible to classify the potential effectiveness of the different types of iron chelates used in our experiments as a function of two practical approaches: (i) considering the variation of total iron in soil solution over time, MA is the best performing product, followed by HA, CA, SA, DTPA, EDTA, and ferrous sulfate in the order listed and (ii) considering the capacity of the different iron chelates to maintain the fraction of chelated iron (ortho‐ortho isomers for HA, MA, SA, and CA and total chelated iron for EDTA and DTPA) in soil solution, the order is: SA > CA > HA > MA > EDTA ≈ DTPA. This result, that is related to the nature of the chelate and does not depend on the degree of chelated Fe in the products, indicates that SA and CA might be very efficient products to correct iron chlorosis. Finally, our results also indicate the suitability of this soil incubation methodology to evaluate the potential efficiency of iron compounds to correct iron chlorosis.     

Iron Homeostasis in Plants: Sensing and Signaling Pathways

Abstract

Since iron is both an essential element as well as a potential toxin, it is a nutrient which on the one hand fulfils many important functions in plants but on the other can cause severe cell damage as a consequence of the formation of reactive hydroxyl radicals. Uptake of iron, its concentrations within particular tissues, and its subcellular distribution is therefore subject to careful control. In addition, the low bioavailability of iron in most soils necessitates the mobilization of sparingly soluble iron compounds. This has led to the evolution of concerted responses that assist in maintaining an adequate supply of iron for plant roots. These responses comprise morphological changes, such as the development of extra root hairs, formation of rhizodermal transfer cells, and induction of cluster roots, as well as induction of genes coding for enzymes involved in the mobilization and uptake of nutrients. Investigations at the protein, mRNA, and structural level showed that both systemic responses, involving transmission of long distance signals, and external nutritional signals, inducing localized responses, are involved in the complex control of iron homeostasis. A number of components have been identified at the molecular level, but the interplay between these components and the signal transduction cascades leading to an iron status within an adequate range are largely unknown. This review summarizes the available data that explain how these processes are coordinated to maintain a continuous and acceptable Fe supply despite changing environmental conditions.     

Pattern of supply affects boron toxicity in barley

Barley (Hordeum vulgare L., cv. Stirling) was grown in pots of a sandy soil to which six levels of boron (B) were added presowing, during stem elongation, or during ear emergence. The pattern of B supply affected the development of leaf injury and other symptoms of B toxicity, the accumulation of B in the grain and in the whole shoots (WS) at maturity, and the relationships between the concentrations of B in the grain and in the WS at maturity and yield. Critical toxic concentrations (CTC) of B in plant tissues were found to vary from approximately 2 to 15 μg/g, and from approximately 50 to 420 μg/g, respectively. The findings of this experiment suggest that barley plants can accumulate relatively high levels of B and express severe levels of leaf injury and other symptoms of B toxicity in the latter stages of growth with relatively small effects on grain yield. They also suggest that the grain and the WS sampled at maturity are not suitable tissues for the diagnosis of yield depressions due to B toxicity in Stirling barley.     

Comparison of Various Analysis Methods for Determination of Iron Chlorosis in Apple Trees

Abstract

The objective of this study was to compare iron (Fe) concentrations (mg kg^?1) of the leaves measured by different methods and to determine the most suitable method to be used in evaluation of iron chlorosis in apple trees. For this purpose, green and chlorotic leaves were collected from 76 apple orchards in 1998 and 1999. Iron concentrations (mg kg^?1) of dried leaf samples were measured with 4 different methods, 1 N HCl (Method 1), 0.1 N HCl (Method 2), 0.005 M DTPA (Method 3), and 1.5% o-phenanthroline (Method 4). Total Fe concentrations (mg kg^?1) of dried leaf samples were also analyzed. Total chlorophyll and peroxidase enzyme activity in fresh leaf samples were measured. The total chlorophyll, peroxidase enzyme activity, Fe concentrations (mg kg^?1) determined by Method 1, Method 3, Method 4, and total Fe concentrations (mg kg^?1) of green leaves were higher than those of chlorotic leaves. On the other hand, no significant difference was found between Fe concentrations (mg kg^?1) of green and chlorotic leaves, measured with Method 2. Significant relationship observed amongst chlorophyll concentrations, peroxidase enzyme activity, and Fe concentrations (mg kg^?1) of samples suggests that 1 N HCl method was the most suitable method amongst the methods used in this study for apple trees.     

Growth and nutrient uptake of peach seedlings with varying magnesium concentrations at low pH 1

Soils of the peach growing region of the Southeastern Coastal Plain are highly leached and excessively acid, with inherently low concentrations of subsoil magnesium (Mg). A greenhouse experiment was conducted to determine the effects of varying Mg concentrations at low pH on growth and Mg uptake of three peach seedling cultivars commonly used as rootstock in the region. Seedlings of ‘Lovell’, ‘Elberta’, [Prunus persica (L.) Batsch] and ‘Nemaguard’ [Prunus persica (L.) Batsch X Prunus davidiana Carriere] were grown for 36 days in nutrient solution containing 9, 21, 42, 84, 167, 333, and 667 μM Mg. Magnesium concentration in solution did not increase lateral length, number of laterals, trunk cross‐sectional area, or root volume. All terminal growth responses were cultivar related. Magnesium concentration in the leaves, stems, and roots were increased either by quadratic or cubic relationship with solution Mg concentration while Mg uptake rate was increased linearly with solution Mg concentration with all three seedling cultivar. Uptake rates of calcium, manganese, and zinc, and tissue concentrations of phosphorus, manganese, and zinc decreased with increasing Mg concentrations in nutrient solution. Predicted Mg uptake rates by‐regression analysis revealed a cubic uptake isotherm for Nemaguard and a quadratic isotherm for Elberta. Predicted tissue Mg concentration followed similar patterns of accumulation for leaves and stems, but root Mg concentration followed a cubic uptake isotherm for all three seedlings. The linear Mg uptake at low pH may be an important physiological characteristic that enables Lovell seedlings to outperform either Elberta or Nemaguard when used as a rootstock in the southeastern soils.     

小麦与花生间作改善花生铁营养的效应研究

采用砂-土联合培养根箱试验装置,模拟田间试验研究石灰性土壤小麦与花生间作改善花生Fe营养的效应结果表明,石灰性土壤高pH和高CaCO3是导致花生缺Fe黄化的主要原因。叶片已发生黄化的花生与小麦间作可明显改善花生缺Fe症状,间作16d后花生根际土壤有效铁含量、花生新叶叶绿素和活性Fe含量均显著提高。小麦与花生间作对改善花生Fe营养的效应可能与缺Fe小麦根分泌的Fe载体对土壤中Fe活化有关。     

The central role of microbial activity for iron acquisition in maize and sunflower

 Maize (Zea mays L.) and sunflower (Helianthus annuus L.) grown on a calcareous soil showed poor growth and/or were chlorotic in spite of abundant Fe in the roots. It has been hypothesized that microbial siderophores chelate Fe (III) in the soil, and that in this form Fe is transported towards the root apoplast. On the calcareous soil, total and apoplastic root Fe concentrations were high, probably because of a high apoplastic pH depressing Fe (III)-reductase activity and thus the Fe²⁺ supply to the cytoplasm. On the acidic soil, total and apoplastic root Fe concentrations were low, probably because of a low apoplastic pH favouring Fe (III) reduction, hence plants showed no Fe-deficiency symptoms. The main objective of the present work was to investigate the role of microbial soil activity in plant Fe acquisition. For this purpose, plants were grown under sterile and non-sterile conditions on a loess loam soil. Plants cultivated under non-sterile conditions grew well, showed no Fe-deficiency symptoms and had fairly high Fe concentrations in the roots in contrast to plants grown in the sterile medium. Low root and leaf Fe concentrations in the axenic treatments indicated that the production of microbial siderophores was totally suppressed. Accordingly, sunflowers were severely chlorotic and this was associated with very poor growth, whereas in maize only growth was drastically reduced. In maize under sterile conditions, root apoplastic and total Fe concentrations were not as low as in sunflowers, which may have indicated that phytosiderophores produced in maize partly sustained Fe acquisition, but due to poor growth were not as efficient in supplying Fe as microbial activity under natural conditions. It may be therefore assumed that in natural habitats soil microbial activity is of pivotal importance for plant Fe acquisition. Received: 11 March 1999     

诊断油菜缺硼的土壤硼素临界范围

Relationships between seed yields of oilseed rape(Brassica napus L.) and extractable boron concen-trations in three soil layers(A,P and W) were investigated through ten experiments on three types of soils(Alluvic Entisols,Udic Ferrisols and Sagnic Anthrosols) in northern,Western and middle Zhejing Province.Among several mathematical models used to described the relationships,the polynomial equation,y=a bx cx^2 dx^3,where y is the yield of oilseed rape seed and x the extractable boron concentration in P layer of soil,was the best one.The critical range of the concentrations corresponding to 90% of the maximum oilseed rape yield was 0.40-0.52 mg kg^-1,The extractable boron concentration of the P layers of the soils was the most stable,The critical range determined was verified through the production practices of oilseed rape in Zhejiang and Anhui provinces.     

The effects of autumn foliar applications of boron and urea on flower quality,yield, boron and nitrogen reserves of apricot

In order to determine the effects of autumn foliar application of boron (B) and/or urea on abortive flower ratio, yield, fruit weight, total soluble solid (TSS) and B and nitrogen (N) contents of reserves of non-irrigated apricot trees, field experiments were carried out between 2009 and 2012 in Malatya province of Turkey. The application of B and/or urea stimulated perfect flower development, B and N accumulation and resulted in significant yield increase. Boron, urea and B+urea applications increased fruit yield by 33.1%, 26.1% and 26.9%, decreased abortive flower ratio by 34.6%, 27.1% and 35.9% compared to the control, respectively. In addition, B and N contents of wood, bark and buds of apricot trees were significantly increased by B and/or urea treatments. It was also observed that B and N contents were the highest in bud compared to wood and bark in all treatments. The highest B (16.53 ppm) and N (1.56%) contents were determined to buds in B+urea treatment. The results of this study suggest that autumn foliar B and/or urea application have the potential to decrease abortive flower ratio and increase the yield and B and N contents of reserves of apricot trees under non-irrigated conditions.     

Differential response of foliage plants to iron deficiency

Eight species of tropical foliage plants were screened to determine their response to Fe‐stress conditions. Plants were grown for 120 days in modified Hoagland's nutrient solution at pH 6.3 containing either 0, 0.22 or 5.52 mg/liter Fe (as Fe⁺³‐HEEDTA). Araucaria heterophylla and Dracaena marginata showed leaf chlorosis and decreased growth at 0 and 0.22 mg/liter Fe. Ficus benjamina and Nephrolepis exaltata ’Bostoniensis’ showed little or no chlorosis or growth differences at either 0 or 0.22 mg/liter Fe. Over a 3 week period, F. benjamina and N. exaltata ’Bostoniensis’ decreased nutrient solution pH approximately 1 to 1.5 units lower than either D. marginata or A. heterophylla at all Fe levels. Codiaeum variegatum var. pictum, Dieffenbachia maculata ’Camille’, Epipremnum aureum, and Philodendron scandens oxycardium were intermediate in growth, chlorosis and lowering of the nutrient solution pH. One explanation for the differential Fe response between these species may be their ability to lower pH of the rhizosphere.     

Effects of solution pH on the growth and chemical composition of rice plants

Rice plants were grown in solution culture for a period of five weeks at pH's ranging from 3.5 to 8.5. Maximum dry matter was obtained at pH 5.5, but substantial reductions in the growth of tops and roots were observed at pH's of 3.5 and 8.5. At pH 3.5, both leaves and roots were short and unhealthy. The roots were thickened with lateral root growth severely inhibited. At pH 8.5, the youngest leaves developed chlorotic symptoms with roots being coarse and discoloured.

Plant concentrations of essential elements were adequate for normal plant growth at pH 5.5. Iron concentration in plant tops substantially decreased with increase in solution pH, but a reverse trend was observed for roots. The concentrations of other elements progressively increased in plant tops and roots with increasing pH.     

Interaction of a Foliar Application of Iron HEDTA and Three Postemergence Broadleaf Herbicides with Soybeans Stressed from Chlorosis

Abstract

Soybeans stressed from chlorosis are often treated with postemergence herbicides to coincide with susceptibility of control of weed seedlings. Three postemergence herbicides, acifluorfen, imazamox, and lactofen were applied to chlorosis stressed soybeans with and without iron HEDTA. At one location, iron amendment resulted in lower yields with acifluorfen and lactofen. Yields were higher with iron amendment in the imazamox treatment, but lower crop injury and poorer weed control was also observed, suggesting that iron amendment antagonized imazamox. Iron amendment did not reduce visual symptoms of chlorosis, herbicide injury, nor did it result in yield increase at any location. Application of iron HEDTA with these herbicides would not be recommended for chlorosis relief.     

Effect of Trichoderma asperellum strain T34 on iron nutrition in white lupin

The production of chelating compounds (siderophores) by microorganisms increases Fe availability to plants. The main objective of this work was to study the effect of Trichoderma asperellum strain T34, a commercially available biocontrol agent, on Fe availability to white lupin (Lupinus albus L). To this end, experiments involving three factors [viz. growing medium (siliceous or calcareous), Fe supply (ferrihydrite enrichment and no enrichment), and inoculation with T34] were performed.Chlorophyll meter readings and concentration and total content of Fe in plants grown on the siliceous medium were decreased by T34, but only in the absence of ferrihydrite. This suggests a potential competition between plants and T34 for Fe under conditions of restricted availability. By contrast, T34 increased the Fe concentration in the aerial parts of lupin plants grown on the calcareous medium. In ferrihydrite-enriched calcareous media, T34 decreased chlorophyll content of the plants. This cannot be ascribed to depressed Fe nutrition or other nutrient deficiency. Inoculation with T34 increased peroxidase (ferrihydrite-enriched calcareous media and siliceous media without ferrihydrite) and catalase activities, but decreased the peroxidase to catalase activity ratio. This effect on the activities of Fe-containing enzymes, which was not the sole result of increased Fe availability to plants, accounts for the disparate relationship between SPAD meter readings and Fe concentrations in plants in inoculated and non-inoculated media. Based on them, a higher Fe concentration in aerial parts was required for similar SPAD meter readings in plants with T34 than in those without T34. This may have resulted from an increased activity in Fe-containing enzymes (particularly catalase) reducing the availability of free Fe for chlorophyll synthesis, which is consistent with the significantly decreased SPAD readings by the effect of T34 in ferrihydrite-enriched calcareous media.