蚯蚓粪肥在农业生产中的应用效果及研究进展

蚯蚓粪肥是将经蚯蚓消化有机废物产生的排泄物进行堆肥而制成的有机肥料,故含有丰富的营养物质和有益微生物.近年来,作物生产的应用效果表明,蚯蚓粪肥能改善土壤结构和微生物种群,促进作物生长、提高作物产量和农产品品质,还能增强植物抵抗病虫害能力,降低土壤中有毒重金属对植物危害.本文对蚯蚓粪肥的作用及相关研究进展进行综述,得出了...     

Vermicompost from municipal sewage sludge affects growth and mineral nutrition of winter rye (Secale cereale) plants

The aim of the study was to investigate sewage sludge vermicompost application effects on growth and mineral nutrition of winter rye (Secale cereale L.) plants as compared to two initial levels of mineral nutrient availability, pure sand and sand enriched with inorganic nutrients at the optimal level. Addition of sewage sludge vermicompost significantly inhibited rye seed germination at 20 to 50% sand substitution independently on mineral nutrient supplement. Growth inhibition was evident at early stages of plant development. However, at the later stages, sewage sludge vermicompost acted both as mineral fertilizer and plant growth-promoting agent. Significant stimulation of mineral uptake was seen only at high rates of vermicompost substitution (40 and 50%) already causing decrease in shoot dry matter accumulation. Vermicompost substitution resulted in a significant increase of leaf chlorophyll content. Beneficial effect of sewage sludge vermicompost in conditions of optimal mineral supply can result mainly from plant growth-promoting activity.     

Vermicomposting leachate as liquid fertilizer for the cultivation of sugarcane (Saccharum sp.)

In vermicomposting, the main product is the worm casts, but a leachate is generated that contains large amounts of plant nutrients. This leachate is normally diluted to avoid plant damage. We investigated how dilution of vermicompost leachate combined with different concentrations of nitrogen (N) - phosphorus (P) - potassium (K) triple 17 fertilizer, and polyoxyethylene tridecyl alcohol as dispersant and polyethylene nonylphenol as adherent to increase efficiency of fertilizer uptake, affected sugarcane plant development. The vermicomposting leachate with pH 7.8 and electrolytic conductivity 2.6 dS m^?1, contained 834 mg potassium (K) l^?1, 247 mg nitrate (NO₃^?) l^?1 and 168 mg phosphate (PO₄^3?) l^?1, was free of pathogens and resulted in a 65% germination index. Vermicompost leachate did not inhibit sugarcane growth and mixed with 170 g l^?1 NPK triple 17 fertilizer resulted in the best plant development. No dispersant or adherent was required to improve plant height and stem development.     

Effect of Vermicompost on Growth,Essential Oil,and Health of Thymus Vulgaris

In this study, the effect of various vermicompost substitutions (0, 25, 50, and 75%) was investigated on germination, growth and development, photosynthetic parameters, the essential oil content, and disease resistance of Thymus vulgaris. Results showed that 25% vermicompost substitution promoted the best seedling emergence indices, whereas maximum length, fresh and dry weight of aerial parts and root, chlorophyll and carotenoid contents, photosynthetic efficiency, and the highest essential oil content were observed in 50% vermicompost substitution. Vermicompost was an effective biocontrol agent against Fusarium oxysporum and Phytophthora infestans. Suppression of pathogens by vermicompost was enhanced progressively with increasing rates of substitution of vermicompost, and the highest suppression obtained in 75% vermicompost substitution. The disease protection correlated to development of activities of defense-related enzymes including β-1,3-glucanase, phenylalanine ammonialyase, polyphenol oxidase and peroxidase, and total phenolics content. These results suggest the potential of vermicompost in promoting of plant yield as well as inducing systemic resistance in Thymus vulgaris.     

The influence of vermicompost water extracts on growth of plants propagated by cuttings

Abstract

Water extracts from vermicomposts, commonly known as “teas,” contain plant available nutrients and plant growth regulators. Their applications on plants have shown to increase seed germination, plant growth and yield, and suppress diseases. This experiment evaluates the effects of vermicompost teas, extracted from food and paper-waste vermicompost, on the rooting characteristics of stem cuttings. Stem cuttings from sugarcane and mint, and leaf cuttings from begonia were treated with four concentrations of vermicompost teas from food wastes: 1%, 2%, 5%, and 10%, compared with water only control and a commercial rooting hormone, Dip ‘N Grow, arranged in randomized complete block design with 10 replications. Rooting characteristics such number of roots, root density, and length of roots were assessed. Results showed that rooting of the stem cuttings from three plants responded differently to applications of vermicompost teas. Vermicompost teas applied at 2% showed as the optimum concentration for sugarcane and mint, while 1% vermicompost tea was optimum for begonia leaf cuttings. The increased rooting in stem cuttings was due to the combination of auxins, cytokinins, GA, and humic acids found in vermicompost teas extracted from food and paper waste vermicompost. Further studies are needed to evaluate whether interactions exist between concentration and length of application time.     

Effects of Vermicompost and Vermiwash on Plant,Phenolic Content,and Anti-oxidant Activity of Mexican Pepperleaf (Piper auritum Kunth) Cultivated in Phosphate Rock Potting Media

The aim of this investigation was to determine the effect of vermicompost, vermiwash, and phosphate rock on plant, total phenols, flavonoids, and anti-oxidant activity in Piper auritum Kunth leaves. P. auritum plants were obtained from cuttings and were planted according to the Box-Behnken experimental design with three repetitions at the central point. The factors and levels were vermicompost (10, 20, and 30 g plant^?1), vermiwash (5, 10, and 15 mL plant^?1), and phosphate rock (1, 2, and 3 g plant^?1). Plant growth parameters (plant height, stem diameter, leaves number) and chlorophyll content were measured 1 month after treatment applications. Total phenols, total flavonoids, and 1,1-diphenyl-2-picryl-hydrazyl radical scavenging activity was measured after 4 months. Vermicompost, vermiwash, and phosphate rock had no statistically significant effect on plant growth. Plant height, stem diameter, leaves number, chlorophyll, innermost number, fresh weight stem, fresh weight leaves, fresh weight root, dry weight stem, dry weight leaves, and dry weight root were not different among treatments. Total phenolic compounds were statistically affected for both vermiwash and phosphoric rock (p < 0.05) and the anti-oxidant activity decreased by vermicompost addition. The application of 15 mL plant^?1 vermiwash, 1 g phosphate rock, and 20 g vermicompost plant^?1 increased the total phenol content.     

Nutrition and biochemical responses of chickpea (Cicer Arietinum L.) to vermicompost fertilizer and water deficit stress

Vermicompost can have an effective role in plant growth and nutrition. It can also reduce the harmful effects of stress caused by environmental factors. This study was a factorial experiment with a completely randomized plot design and three replications. The results showed that the calcium concentration (+40% +39%) of root and calcium (+71% +67%) and potassium contents (+49% +59%) of leaf under conditions of moderate and severe water stress increased under vermicompost treatments at the ratio of 30%. Sodium absorption (?37% ?21%) in moderate and severe conditions was significantly reduced by vermicompost treatment. In moderate and severe water stress, the growth medium of 30% vermicompost significantly enhanced proline (+39%) and soluble protein content (+28%) compared to the control. In conclusion, our results proved vermicompost fertilizer due to its structural properties and components, served to reduce the negative effects of water stress by increasing the absorption of calcium (Ca) and potassium (K), and decreasing sodium (Na) absorption that resulted in increased evaluations for proline and protein content.     

Effects of Vermicompost Tea (Aqueous Extract) on Pak Choi Yield,Quality, and on Soil Biological Properties

This study investigated the effects of vermicompost tea (aqueous extract) on yield and chemical quality of pak choi (Brassica rapa cv Bonsai, Chinensis group) grown in three media (two soils and a peat-perlite medium) under two fertilizer regimes (compost and synthetic fertilizer). The impacts of tea application on the chemical and biological properties of the growth media were also investigated. Vermicompost teas were prepared using various extraction methods (non-aerated, aerated, aerated with additives) with 1:10 (v:v) chicken manure-based vermicompost to water dilution and applied weekly at the rate of 200 mL plant^?1 for 4 weeks. Application of vermicompost tea increased plant production, total carotenoids and total glucosinolates in plant tissue. This effect was most prominent under compost fertilization. Total phenolic was lower in vermicompost tea treated plants compared to those treated with only mineral nutrient solution and the water control. Vermicompost tea improved mineral nutrient status of plants and media, and enhanced the biological activity of the media. Variability in yield and chemical quality of plants across treatments was explained largely by variability in tissue N uptake and dry matter accumulation. Dehydrogenase activity and soil respiration of vermicompost tea-treated growth media were approximately 50% higher than untreated media. This study confirmed that vermicompost tea can positively influence plant yield and quality and increase soil biological activity in multiple soil types.     

Responses of Common Pot Grown Flower Species To Commercial Plant Growth Media Substituted With Vermicomposts

Waste management is an increasingly important problem in many developed countries and much effort is currently being expended into identifying novel uses for waste products. We consider the effects of substituting proportions of earthworm-digested composted green waste (vermicompost) into commercial peat based growing media on the germination, growth, and productivity of Helianthus annuus, Cosmos bipinnatus and Eschscholzia californica. The percentage germination, time to flowering, seed production, overall heights and biomass were measured for all three species in treatments of commercial peat based growing media where the growth medium was substituted with vermicompost at rates of 0%, 20%, 40%, 60% and 100%. In addition, the total numbers of inflorescences was recorded in multiple flowering species. Results for Helianthus suggest that while vermicompost additions induced small species specific growth responses in germination rate, growth rate, overall height and total biomass, none were statistically significant. For Cosmos there was a significant increase in early plant growth rate and in the final flower numbers at substitution rates of 20% and 40% vermicompost. The total biomass and numbers of flowers produced by Eschscholzia was decreased significantly by substitution of 20% and 40% vermicompost. We conclude that the plant growth enhancement properties of this vermicompost seem more species specific than previously reported. Further, in order to preserve consumer confidence in waste-derived horticulture products, vermicompost marketing strategies should acknowledge these findings.     

Plant Growth and Sugars Content of Agave americana L. Cultivated with Vermicompost and Rock Phosphate and Inoculated with Penicillium sp. and Glomus fasciculatum

The objective of this study was to investigate the effect of vermicompost, rock phosphate, Glomus fasciculatum (arbuscular mycorrhizal fungi), and Penicillium sp. (phosphate solubilizing fungi) on growth; mycorrhization percent; fructan, sucrose, glucose, and fructose contents in leaves and stem of Agave americana L. Experiments were done according to an orthogonal experimental design L₈ with four variables at two levels: vermicompost (0 or 10 g per plant), rock phosphate (0 or 1 g per plant), G. fasciculatum (0 or 1 × 10⁶ spores per plant), and Penicillium sp. (0 or 1 × 10⁹ spores per plant). Vermicompost affected stem dry weight; the fructan, glucose, and fructose in stem; and mycorrhization percent in roots. Rock phosphate and Penicillium sp. increased the mycorrhization percent. Co-inoculation of Penicillium sp. and G. fasciculatum increased A. americana plant growth. The results obtained suggested a synergistic interaction between the arbuscular mycorrhizic fungi and the phosphate solubilizing fungi.     

Evaluation of vermicompost fertilizer application on growth,nutrient uptake and photosynthetic pigments of lentil (Lens culinaris Medik.) under moisture deficiency conditions

Vermicompost can play an effective role in plant growth and also in reducing harmful effects of various environmental stresses on plants due to its porous structure, high-water storage, having hormone-like substances and also high levels of macro and micro nutrients. Application of vermicompost in soil, especially at the levels of 15 and 25 Wt% significantly increased all studied traits under non-stress conditions. Under temperate and severe stress conditions, vermicompost at 25 Wt% treatment resulted in a significant increase in plant height (+10% +21%), number of pod (+44% +65%), root dry weight (+63% +66%), shoot dry weight (+50% +89%), leaf area (+6% +7%), root area (+65% +35%). The leaf calcium (Ca) (+62% +67%), leaf potassium (K) (+39% +45%) and root Ca (+33% +34%) under temperate and severe stress, increased using of 25% vermicompost. In conclusion, we suggested that application of vermicompost had a positive effect on lentil under moisture deficit stress.     

Effect of vermicompost on some morphological,physiological and biochemical traits of bean (Phaseolus vulgaris L.) under salinity stress

Vermicompost can play an effective role in plant growth and development and also in reducing harmful effects of various environmental stresses on plants due to its porous structure, high water storage capacity, having hormone-like substances and plant growth regulators and also high levels of macro and micro nutrients. The aim of this study was to investigate the effects of vermicompost and salinity interactions on some morphological and physiological features and concentration of mineral elements of bean (Phaseolus vulgaris L. cv. Light Red Kidney) cultivar. A factorial experimental with five different volumetric ratios of vermicompost and sand, including to: 0:100; 10:90; 25:75; 50:50 and 75:25 and four levels of salinity [20, 40, 60 and 80 mmol l^?1 sodium chloride (NaCl)], and control was conducted base on Randomized Complete Block Design with three replications. Bean seeds were sowed in plastic pots, the seedlings being sampled 42 days old (flowering stage).The results showed that vermicompost had significant effect on all studied traits under stress and non-stress (p ≤ 0.05).In this experiment, the vermicompost significantly increased the photosynthetic rate and concentrations of potassium (K⁺) and calcium (Ca²)⁺in leaf and root tissues. In salinity levels of 20, 40 and 60 mmol l^?1NaCl, all subjected ratios of vermicompost and in 80 mmol l^?1NaCl the ratios of 10% and 75% vermicompost, significantly ameliorated negative effects of salinity. In both stress and non-stress conditions, using 10% volume of vermicompost is recommended to improve the growth of bean plants.     

Vermicompost and Vermiwash Minimized the Influence of Salinity Stress on Growth Parameters in Potato Plants

Potato (Solanum tuberosum L.) is the fourth major crop worldwide after cereals. Some producers use irrigation water with high salinity, which consequently decreases the agronomic yield and potato quality. The aim of this investigation was to determine the effect of vermicompost and vermiwash on plant growth and tuber yield and characteristic traits in Solanum tuberosum L. plants and tubers subjected to salinity stress. A surface response experimental design with three replicates using a central point and 15 treatments was used with vermicompost at 300, 580, and 860 g plant^?1; vermiwash at 5, 10, and 15 ml plant^?1; and salinity stress with 15, 20, and 25 mM of NaCl levels. Plant physiological measurements included plant height (cm), stem diameter (mm), and plant fresh and plant dry weight (g). Six months after planting, measurements on tuber fresh weight, pH, electric conductivity, and °Brix were carried out. The addition of vermicompost and vermiwash minimized the influence of salinity stress on growth parameters and tuber characteristics in potato plants. Vermicompost (580 g plant^?1) plus vermiwash (15 ml plant^?1) induced a greater plant height and stem diameter. Plants amended with vermicompost (860 g plant^?1), vermiwash (15 ml plant^?1), and salinity stress (15 mM) had higher pH values, whereas electrical conductivity value in potato tubers decreased.     

Vermicomposting with rock powder increases plant growth

The growth of earthworms in substrates enriched with rock (gneiss and steatite) powder, and the potential of vermicomposting in increasing solubilisation of minerals present in rock powder and in promoting plant growth were evaluated. Cattle manure (400 g), was enriched with 0, 5 and 20% of gneiss or steatite powder. Each pot with this mixture received nine earthworms (Eisenia andrei), at a density of 1000 indiv. m⁻³. After 60 d, earthworms were collected, counted and weighed (fresh and dry). Maize was cultivated in a greenhouse in pots with an Oxisol that was fertilised with the vermicompost obtained above. Treatments with Oxisol fertilised with gneiss or steatite only and unfertilised soil were used as controls. Shoot length was measured weekly from the soil surface to the tips of the leaves. After 73 d, the plants were harvested, the roots washed from the soil and shoots and roots dried and weighed. Plants fertilised with vermicompost enriched with rock powder were taller and heavier than plants fertilised with non-enriched vermicompost. Plants grown on soil fertilised with rock powder but not with vermicompost were larger than plants grown on unfertilised soil. Vermicompost enriched with steatite powder resulted in a larger effect on plant growth than the mere sum of applying vermicompost of non-enriched manure and steatite alone to the soil. A similar, but non-significant effect was also observed for gneiss. The different effects between gneiss and steatite may be associated with the lower resistance to chemical weathering of steatite minerals compared to gneiss minerals, as well as the former being softer than the latter. The effect of vermicompost on the optimisation of nutrient release from silicate rocks seems to depend on the rock type.     

Influence of Growing Medium and Seed Size on Germination and Seedling Growth of Pinus gerardiana Wall.

Pinus gerardiana Wall. is an important nut-producing pine having restricted distribution in the world. It has been observed that natural regeneration in the species is extremely poor or entirely lacking. The species has erratic and infrequent seed years, dormancy-related problems, and slow growth of seedling, which reduces its regeneration process in natural habitats. Therefore, we investigated the effect of growing medium and seed size on germination and seedling growth of the Pinus gerardiana. The seeds were categorized into two sizes, viz, small (<2.35 cm) and large (>2.35 cm) and five growing medium treatments were used, viz, Soil:Sand:FYM; Soil:Sand:Moss:FYM; Soil:Moss:Vermicompost; Soil:Sand:Vermicompost; and Soil:Sand:Moss:Vermicompost, for assessing their impact on germination and seedling growth. It was observed that, among different growing medium treatments, higher germination and seedling growth parameters were recorded, when Soil:Sand:Moss:Vermicompost was used for the study. Between different seed sizes, higher germination and seedling growth were recorded when seed size was large.     

扁蓿豆水浸提液对其种子萌发自毒作用研究

为了明确扁蓿豆是否存在自毒效应，以期为扁蓿豆科学种植提供理论依据。以扁蓿豆种子为受体，采用室内培养皿种子萌发方法，研究了不同质量浓度(0、0.001、0.025、0.050、0.100 g/mL)扁蓿豆(Medicago ruthenica)地上部分和地下部分水浸提液的自毒作用。结果表明，扁蓿豆植株地上部分与根部水浸提液对其种子发芽率、发芽势、发芽指数、胚根长、胚芽长和单株鲜质量具有一定的抑制作用，总体表现为在质量浓度0.100g/mL下抑制作用最强。扁蓿豆地上部分水浸提液处理在0.100 g/mL质量浓度下发芽率较对照蒸馏水显著降低了39.00%(P < 0.05)，种子发芽指数较对照蒸馏水降低了82.00%；根部水浸提液在0.100 g/mL质量浓度下胚根长较对照蒸馏水显著降低了50.00%。各指标的化感效应指数与质量浓度均呈负相关。     

Vermicompost Leachate as a Promising Agent for Priming and Rejuvenation of Salt-Treated Germinating Seeds in Brassica Napus

Salinity is a major constraint hampering germination and early seedling growth, especially in aged seed lots. Any rejuvenation treatment improving salt resistance at these crucial developmental stages will be of special interest. Two sets of experiments were performed in Brassica napus to precise the impact of vermicompost leachate (VCL) on seed germination in the presence of NaCl and to analyze its putative interest as seed priming agent before NaCl exposure. Two seed lots were used: one old seed lot (cv. Libomir) and a recent one (cv. Harry). VCL increased the germination percentage of aged seeds in the absence of NaCl and increased seedling length in both cultivars. VCL had only a minor impact when directly added to the NaCl-containing germinating solution. In contrast, priming with VCL strongly improved subsequent germination in the presence of NaCl in relation to a more efficient management of oxidative stress in both cultivars. The improvement of salinity resistance provided by VCL priming was not due to modification in ion or proline content. It is concluded that VCL may act as a rejuvenation agent invigoring old seed lots and as an efficient priming agent for improvement of salinity resistance at the germination stage. Valuable properties of VCL are discussed in relation to the simultaneous presence of several protecting compounds.     

The effects of vermicompost tea on the growth and yield of lettuce and tomato in a non-circulating hydroponics system

The use of vermicompost water extracts (teas) in horticulture to increase plant growth and yield and suppress pests and diseases, is well documented. However, its utilization and effects in hydroponics systems are unknown. Low concentrations of vermicompost teas, produced from food wastes, at rates of 1.6% and 3.2% for lettuce and 0.14%, 0.28% and 0.56% for tomatoes were investigated as additive and supplement in static hydroponic systems. Vermicompost teas significantly increased lettuce yields when concentrations of nutrient solutions were reduced to 25% and 50% of the recommended full rate for nutrient solutions compared to treatments without vermicompost teas. Even lower concentrations of vermicompost teas increased tomato yields significantly as a supplement in reduced nutrient solutions of 50%. The presence of a combination of trace amounts of plant hormones such as auxins, cytokinins, gibberellins and humic acids in vermicomposts teas are likely the responsible factor that increased yields of lettuce and tomato in static hydroponics systems with lower concentrations of nutrient solutions.     

蚯蚓粪与土壤复配比例对基质微生物性状及韭菜生长和品质的影响

【目的】蚯蚓粪是蚯蚓消化有机废弃物产生的均匀颗粒,能够提升土壤肥力,改良土壤结构并促进作物生长。本文初探了蚯蚓粪与土壤的不同配比对基质微生物性状及韭菜生长和品质的影响,为蚯蚓粪的开发利用提供依据。 【方法】2015年4—6月在中国农业大学温室内进行盆栽试验,按蚯蚓粪、土壤质量比 (w/w) 设置4个处理,依次为:0/100% (纯土壤不添加蚯蚓粪,CK);20%/80% (T1);60%/40% (T2);100%/0 (T3)。韭菜收获后,测定了基质养分含量、微生物群落变化、韭菜生物量、可溶性蛋白总量、可溶性糖总量和叶绿素含量。 【结果】1) 随着蚯蚓粪添加比例的增加,基质中的全氮、速效钾和有机质的含量均显著提高;2)蚯蚓粪能显著促进韭菜根系生长,并改善韭菜品质。与对照相比,T1、T2和T3处理中韭菜新根比分别提高5.75%、6.39% 和22.23%;韭菜可溶性蛋白总量提升了1.84～5.97倍,可溶性糖总量提升了1.49～1.60倍 (P < 0.05);3)蚯蚓粪能显著提高基质中的细菌和真菌多样性,并显著增加细菌116、118、130、226、297片段 (P < 0.05),但减少了真菌71、91、153、351片段 (P < 0.05)。 【结论】蚯蚓粪改善了基质的微生物群落结构,提高细菌和真菌的多样性,增加养分有效性,进而促进韭菜生长并改善韭菜品质。蚯蚓粪直接用作基质的效果最好,但从经济角度考虑,蚯蚓粪与土壤按质量百分比20%/80%即可基本达到满意的效果。     

Influence of earthworm culture on fertilization potential and biological activities of vermicomposts prepared from different plant wastes

The present investigation was aimed to analyze influence of earthworm culture on nutritive status, microbial population, and enzymatic activities of composts prepared by utilizing different plant wastes. Vermicomposts were prepared from different types of leaves litter of horticulture and forest plant species by modified vermicomposting process at a farm unit. Initial thermophilic decomposition of waste load using cow‐dung slurry was done in the separate beds. The culture of Eisenia fetida was used for vermicomposting in specially designed vermibeds at the farm unit. The physico‐chemical characteristics, enzyme activities (oxido‐reductases and hydrolases), and microbial population (bacteria, fungi, free‐living nitrogen‐fixing bacteria, actinomycetes, Bacillus, Pseudomonas, phosphate‐solubilizing bacteria and fungi) of vermicomposts were found significantly higher (p < 0.05) than those of control (without earthworm inoculum). The study quantified significant contributions of earthworm culture to physico‐chemical, enzymatic, and microbiological properties of vermicompost and confirmed superior fertilization potential of vermicompost for organic farming. The agronomic utility of vermicompost was assessed on yellow mustard plant in a pot experiment. Pot soil was amended with different ratios (5%, 10%, 20%) of vermicompost and normal compost (without earthworm inoculum). Effects of these amendments on the growth of Brassica comprestis L. were studied. The significant differences (p < 0.05) in the growth of plant were observed among vermicompost‐, compost‐amended soil, and control. Vermicompost increased the root and shoot lengths, numbers of branches and leaves per plant, fresh and dry weights per plant, numbers of pods and flowers, and biochemical properties of plant leaf significantly, especially in 20% amendment. These results proved better fertilization potential of vermicompost over non‐earthworm‐inoculated compost.