Effects of phosphate,nitrate and chloride on calcium,magnesium and manganese content of cigar‐wrapper tobacco

Abstract

Nutrient solutions containing three levels of phosphate, nitrate, and chloride were applied to cigar‐wrapper tobacco (Nicotiana tabacum L.) plants growing in sand culture for a period of 18 days. Concentrations of other nutrient elements in the nutrient solutions were held constant and the solutions were applied to pots as needed to maintain favorable moisture conditions for plant growth. Plants were in the two leaf stage when transplanted and were maintained on a single nutrient solution for 38 days before treatments were started. At the end of an eight weeks growing period, plant leaves were harvested and analyzed for Ca, Mg, and Mn. Dry matter yield was significantly (P=0.01) increased when 2 mM/1 of Ca(NO₃)₂ replaced an equivalent amount of Ca(H₂PO₄)₂ or CaCl₂ in the nutrient solution. Nitrate significantly (P=0.05) increased Ca and Mg content and decreased Mn concentration in leaf tissue in comparison to chloride. Calcium and Mg content were significantly (P=0.05) decreased and Mn content of tobacco leaves was increased by phosphate in comparison to nitrate and chloride.     

Energy dispersive X‐ray fluorescence for rapid potassium,calcium, and chloride diagnosis in barley

Barley (Hordeum vulgare L. cv Doriru) leaf samples were collected from a field comprising three plots, plot F chemical fertilizer treated, plot S receiving sewage sludge and sawdust mixed compost, and plot H receiving sewage sludge and rice husk mixed compost. Relative concentrations of selected elements, potassium (K), calcium (Ca), and chloride (Cl) of young, mature, and old barley leaves were determined by microscopic energy dispersing X‐ray fluorescence (EDXRF). The objective of this investigation was to verify the applicability of EDXRF for rapid nutrient element diagnosis of plants. Typically whole leaves were washed in deionized‐distilled water and dried by ironing for analysis. Intact dried barley leaf sample irradiation was accomplished with X‐rays obtained from an X‐ray tube focused on an area <100 μm of the respective sample specimen surface. The EDXRF provided sufficient sensitivity for relative concentrations of K, Ca, and Cl. Element content data of all the elements investigated, specifically K, resulted in adequate plant nutrient element values to diagnose K insufficiency in barley leaves taken from plants in the sewage sludge receiving plots. Potassium was more densely accumulated in the new leaf than in mature and old leaves in case of plants from the S and H plots. In contrast, such K accumulation was more dense in old and mature leaves than young leaves in case of plants from the F plot. However, Cl and Ca coupling in barley leaves from all of the F, S, and H plots had shown the similar pattern of distribution and followed the order: old > mature > young. Therefore, EDXRF can be an easy, rapid, and practical method for diagnosing the elemental content of plant tissues and thereby help to aid plant growth and development through timely supplements of the required element(s).     

Simultaneous determination of soil aluminum,ammonium‐ and nitrate‐nitrogen using 1 M potassium chloride extraction

Abstract

Determination of soil aluminum (Al), ammonium‐nitrogen (NH₄‐N), and nitrate‐nitrogen (NO₃‐N) is often needed from the same soil samples for lime and fertilizer recommendations, but Al has to be extracted and quantified separately from NH₄‐N and NO₃‐N according to present methods. The objective of this study was to develop a reliable method for simultaneous analyses of soil Al, NH₄‐N and NO₃‐N using a Flow Injection Autoanalyzer. Thirty‐five soil samples from different locations with wide ranges of extractable Al, NH₄‐N and NO₃‐N were selected for this study. Aluminum, NH₄‐N and NO₃‐N were extracted by both 1 M and 2 M potassium chloride (KCl), and quantified using a LACHAT Flow Injection Autoanalyzer simultaneously and separately. One molar KCl was found to be a suitable extractant for all three compounds when compared to 2 M KCl. The 1 M KCl extract proposed could aid in decreasing the costs associated with simultaneous NH₄‐N, NO₃‐N, and Al analyses. Results of those three compounds analyzed simultaneously were not statistically different from those analyzed separately in 1 M KCl solution. This new procedure of simultaneous determination of NH₄‐N, NO₃‐N, and Al increases efficiency and reduces cost for soil test laboratories and laboratory users.     

Effect of ammonium chloride,ammonium sulphate and sodium nitrate on take‐all and grain yield of wheat in southwestern Australia

Five field experiments measured the effect of three sources of nitrogen (N) fertilizer, applied at 45 kg N/ha, on the incidence of take‐all and grain yield of wheat. The N fertilizers were ammonium sulphate, ammonium chloride and sodium nitrate. Compared with the nil N treatment, ammonium nitrogen fertilizer, either as ammonium sulphate (ASdr) or ammonium chloride (ACdr) drilled with the seed, lowered the severity of take‐all. Sodium nitrate topdressed (SNtd) to the soil surface reduced the severity of take‐all in three of the five experiments, while ammonium sulphate topdressed (AStd) reduced the severity in four experiments. Ammonium sulphate and ammonium chloride drilled with the seed were equally effective in reducing the severity of take‐all in three of the five experiments. However, ACdr was more effective than ASdr in reducing the severity of take‐all in one experiment, whereas ASdr was more effective than ACdr in another experiment. In experiments 1 and 5, the reduction in take‐all severity between the ASdr and ACdr treatments did not affect grain yield. Results suggested that grain yield losses from take‐all are most severe where wheat plants are deficient in N. Fertilizers containing chloride are unlikely to control take‐all disease of wheat on soils of southwestern Australia.     

Effect of ammonium sulfate,ammonium chloride and root‐zone acidity on inorganic ion content of tobacco

Tobacco plants (Nicotiana tabacum L. cv NC82) were supplied with (NH₄)₂SO₄, or NH₄Cl at root‐zone pH of 6.0 and 4.5 in hydroponic culture for 28 days. Dry matter accumulation, total N and C content, and leaf area and number were not affected by the NH₄ ⁺ source or root‐zone pH. Plants supplied with NH₄C1 accumulated up to 1.2 mM Cl g DW^‐1, but accumulated 37% less inorganic H₂PO₄ ^‐ and 47% less SO₄ ^2‐ than plants supplied with (NH)₂SO₄. The large Cl^‐ accumulation resulted in NH₄C1 –supplied plants having a 31% higher inorganic anion (NO₃ ^‐, H₂, PO₄ ^‐, SO₄ ^2‐, and Cl^‐) charge. This higher inorganic anion charge in the NH₄C1‐supplied plants was balanced by a similar increase in K⁺ charge. Plants supplied with NH₄Cl accumulated greater concentrations of Cl^‐ in leaves (up to 5.1% of DW) than plants supplied with (NH₄)₂SO₄ (less than ‐% DW). Despite the high Cl^‐ concentration of leaves in NH₄Cl supplied plants, these plants showed no symptoms of Cl^‐ toxicity. This demonstrates that toxicity symptoms are not due solely to an interaction between high Cl^‐ concentration in tissue and NH₄ ⁺ nutrition. The increase in root‐zone acidity to pH 4.5 from 6.0 did not induce toxicity symptoms.     

Effect of ammonium chloride,ammonium sulphate,and sodium nitrate on take‐all and grain yield of wheat grown on soils in South‐western Australia

Five field experiments are described which measured the effect of three sources of nitrogen (N) fertilizer, applied at 45 kg N/ha, on the incidence of take‐all and grain yield of wheat. The N fertilizers were ammonium sulphate, ammonium chloride, and sodium nitrate. Compared with the Nil N treatment, ammonium‐nitrogen fertilizer, either as ammonium sulphate (ASdr) or ammonium chloride (ACdr) drilled with the seed, lowered the severity of take‐all. Sodium nitrate topdressed (SNtd) to the soil surface reduced the severity of take‐all in three of five experiments, while ammonium sulphate topdressed (Astd) reduced the severity in four of the five experiments. Ammonium sulphate and ammonium chloride drilled with the seed were equally effective in reducing the severity of take‐all in three of the five experiments. However, ACdr was more effective than ASdr in reducing the severity of take‐all in one experiment whereas ASdr was more effective than ACdr in another experiment. In these two experiments (1 and 5), the effects of the reduction in take‐all severity between the ASdr and ACdr treatments did not affect grain yield. The results suggest that grain yield losses from take‐all are most severe where wheat plants are deficient in N. Chloride containing fertilizers are unlikely to control take‐all disease of wheat on soils of southwestern Australia.     

Comparison of mehlich 3, mehlich 1, ammonium bicarbonate‐DTPA, 1.0M ammonium acetate,and 0.2M ammonium chloride for extraction of calcium,magnesium, phosphorus,and potassium for a wide range of soils

Abstract

Extractants employed for routine soil analysis vary from one laboratory to another. Lack of a universal soil extractant is a serious limitation for interpretation of analytical results from various laboratories on nutritional status of a given soil. This limitation can be overcome by developing functional relationships for concentrations of a given nutrient extractable by various extradants. In this study, extractability of Ca, Mg, P, and K in a wide range of soils (0–15 cm) from citrus groves in Florida representing 21 soil series, with varying cultural operations, were compared using Mehlich 3 (M3), Mehlich 1 (M1), ammonium acetate (NH₄AOc), pH = 7.0 (AA), 0.2M ammonium chloride (NH₄Cl), and ammonium bicarbonate‐DTPA (AB‐DTPA) extractants. Soil pH (0.01M CaCl₂) varied from 3.57 to 7.28. The concentrations of Ca or Mg extractable by M3, M1, AA, and NH₄Cl were strongly correlated with soil pH (r² = 0.381–0.482). Weak but significant correlations were also found between AB‐DTPA extractable Ca or Mg and soil pH (r² = 0.235–0.278). Soil pH relationships with extractable K were rather weak (r² = < 0.131) for M1 and NH₄Cl but non‐significant for M3, AB‐DTPA, and AA. Concentrations of Ca, Mg, and K extractable by M3 were significantly correlated with those by either M1, AA, or NH₄Cl extractants. Mehlich 3‐P was significantly correlated with P extractable by M1 extractant only. Mehlich 3 versus AB‐DTPA relationship was strong for K (r² = 0.964), weaker for Mg and P (r² = 0.180–0.319), and non‐significant for Ca. With the increasing emphasis on possible use of M3 as an universal soil extractant, data from this study support the hypothesis that M3 can be adapted as a suitable extractant for routine soil analysis.     

Influence of chloride on the uptake and trans‐location of phosphorus in potato

Both chloride (Cl) and sulphate (SO₄) were found to impair the uptake of phosphorus (H2³²PO₄) through the roots of potato in a hydroponically conducted experiment. Radioactive phosphorus (³²P) was used as the marker. The presence of either Cl and SO₄ alone in the nutrient solution made no difference on the impairment. Both ions also inhibited the long‐distance transport of P from roots to shoots. The inhibition by Cl was much greater than that by SO₄, and increased with the concentration of Cl rising from 0 to 15.5 mM in solution.     

Use of a solid‐state chloride electrode for chloride determinations in soil extracts

Abstract

Studies were conducted to evaluate the use of a solid‐state chloride electrode for chloride determinations in soil extracts. The solid‐state chloride ‐electrode was used as an end point detector in the titration of chloride with AgN0₃ and direct reading of chloride in solution. The solid‐state electrode gave results very similar to those obtained with a silver electrode (r = 0.999) when used as an end point detector. Values obtained using the solid‐state electrode as a direct reading electrode were also well correlated (r = 0.998) but were slightly higher than results obtained by titration with a silver electrode.     

A re‐examination of exchangeable acidity as extracted by potassium chloride and potassium fluoride

Abstract

In order to better understand some of the factors likely to affect measurements of KC1 extractable acidity, experiments were conducted using synthetic solutions and extracts from a wide range of contrasting soils. The reagents used for measuring exchangeable acidity (i.e., KC1 and KF) were also examined to evaluate the effects of chemical impurities on acidity measurements. Two commonly used titrimetric methods were adapted and tested to determine the accuracy and precision of acidity measurements. The exchangeable acidity of soil extracts was investigated by extraction methods, extractant concentration, and extractant volume. Results from the soil extract experiments indicated that continuous shaking has no significant effect on acidity measurements. Filtration, however, is critical, especially for acidic organic‐rich soils, since aluminum (Al) ions can be lost during centrifugation. Extractant concentration and volume had variable effects on the acidity measurements for individual soils. In general, the modified Yuan's method is preferable to the modified Thomas’ method for estimating exchangeable Al. To ensure successful determination of exchangeable acidity, we recommend using a wider KCl:soil ratio (>15:1, v/w) for organic soils with low base saturation and allophanic Andisols. In sum, potassium chloride and potassium fluoride extraction for estimating exchangeable acidity is applicable for most soils.     

Effect of applying calcium chloride and three milk supplements to poinsettia ‘Prestige Red’

Some poinsettia (Euphorbia pulcherrima Willd. ex Klotzsch) cultivars are susceptible to bract calcium (Ca) deficiency in developing bracts. In this study, we evaluated the efficacy of foliar uptake of Ca from milk-based products plus calcium chloride (CaCl₂) as a potential Ca source. Weekly foliar applications of 237 mL L^?1 whole milk, 80 mL L^?1 powdered milk, 30 mL L^?1 condensed milk, 0.94 g L^?1 CaCl₂, or distilled water (used as a control) were applied to runoff of ‘Prestige Red’ for four weeks. The four largest bracts with petioles on three different inflorescences and three leaves with petioles below the transitional leaf per inflorescence were collected per pot. None of the treatments affected bract or leaf Ca concentration. Powdered milk treatments resulted in a higher concentration of zinc (Zn) in bracts and aluminum (Al) in bracts and leaves. White residue remained on the bracts and leaves after treatment with powdered milk, which would reduce marketability.     

Cell neutrality in sodium chloride‐treated leaves and calli of the wilty mutant lycopersicon esculentum flacca and the wild species lycopersicon peruvianum

Some parameters involved in cell neutrality (inorganic solute, cation‐anion balance, proline and organic acid contents linked to oxidative respiration, and PEPCase activity) were determined in salinized plant leaves and leaf calli from two tomato species, the wilty mutant Lycopersicon esculentum cv flacca, and the wild relative L. peruvianum. Callus sensitivity was associated to membrane integrity and cell neutrality; while whole plant sensitivity was linked to nutritional unbalance and energy supply, both factors affecting the maintenance of the cell neutrality by means of ion translocation/compartimentalization.     

Rotenone and rotenoids in cubè resins, formulations, and residues on olives

Rotenone and rotenoids (deguelin, beta-rotenolone (12a beta-hydroxyrotenone), tephrosin (12a beta-hydroxydeguelin), 12a alpha-hydroxyrotenone, and dehydrorotenone) were determined in cubè resins and formulations. Cubè resins from Lonchocarpus contain large quantities of deguelin (ca. 21.2%) and smaller quantities of tephrosin (ca. 3.5%) and beta-rotenolone (ca. 3.0%). The composition of commercial formulations may present very different rotenoid contents depending on the extracts used to prepare them. Because these rotenoids also present insecticide activity, the efficacy of these formulations may be very different. The storage stability and photodegradation of some rotenone formulations were studied. Rotenone and rotenoids are very sensitive to solar radiation, which degrades them rapidly, with half-lives in the order of a few tens of minutes. Some formulations show greater disappearance rates than that of cubè resin, indicating that not much attention has been paid to protecting the active ingredients from photodegradation in the formulation. A study on the residues on olives was also carried out to assess not only the rotenone content, but also that of the main rotenoids. At harvest, the residues of deguelin, tephrosin, and beta-rotenolone were 0.10, 0.06, and 0.10 mg/kg, respectively, very similar to rotenone (0.08 mg/kg), and though a few data indicate similar acute toxicity values for deguelin, only rotenone is taken into consideration in the legal determination of the residue.     

Land use effects on microbial biomass C, β-glucosidase and β-glucosaminidase activities,and availability,storage, and age of organic C in soil

Microbial biomass, β-glucosidase and β-glucosaminidase activities, and availability, storage, and age of soil organic C were investigated after 26 years of conversion from sugarcane (Saccharum officinarum) to forest (Eucaliptus robusta or Leucaena leucocephala), pasture (mixture of tropical grasses), and to vegetable cropping (agriculture) in a vertisol in Puerto Rico. Soil organic C (SOC) at 0–100 cm was similar under Leucaena (22.8 kg C/m²), Eucalyptus (18.6 kg C/m²), and pasture (17.2 kg C/m²), which were higher than under agriculture (13.0 kg C/m²). Soil organic N (SON) at 0–100 cm was similar under the land uses evaluated which ranged from 1.70 (under agriculture) to 2.28 kg N/m² (under Leucaena forest). Microbial biomass C (MBC) and N (MBN) of the 0–15-cm soil layer could be ranked as: pasture > Leucaena = Eucalyptus > agriculture. The percentages of SOC and SON present as MBC and MBN, respectively, were nearly 1% in pasture and less than 0.50% in forest under Leucaena or Eucalyptus and agricultural soil. The activity of β-glucosidase of the 0–15-cm soil layer could be ranked as: Leucaena = Eucalyptus > pasture > agriculture; while β-glucosaminidase activity was ranked as: Eucalyptus > Leucaena = pasture > agriculture. The soil δ ¹³C changed from 1996 to 2006 in forest under Eucalyptus (18.7‰ to 21.2‰), but not under Leucaena (20.7‰ to 20.8‰). The soil under Leucaena preserved a greater proportion of old C compared to the forest under Eucalyptus; the former had an increased soil mineralizable C from the current vegetation inputs. The soil under agriculture had the lowest enzyme activities associated with C cycling, lowest percentage of SOC as MBC, highest percentage of SOC present as mineralizable C, and highest percentage of MBC present as mineralizable C compared to the other land uses.     

The determination of ammonium and chloride by an auto‐analyser for the measurement of cation exchange capacity of soils

Abstract

An auto‐analyser method has been developed for the simultaneous determination of NH₄ ⁺ and Cl^‐ in Ca(NO₃)₂/KNO₃ extracts of NH₄Cl treated soils for cation exchange capacity measurements. The method gives satisfactory agreement with manual titration procedures.     

Pharmacological, structural, and drug delivery properties and applications of 1,3-β-glucans

1,3-β-Glucans are a class of natural polysaccharides with unique pharmacological properties and the ability to form single- and triple-helical structures that can be formed into resilient gels with the application of heat and humidity. The pharmacological capabilities of 1,3-β-glucans include the impartation of tumor inhibition, resistance to infectious disease, and improvements in wound healing. Curdlan is a linear 1,3-β-glucan that has been used extensively to study the nature of these helical structures and gels, and Curdlan sulfates have found ongoing application in the inhibition of HIV infection. 1,3-β-Glucan gels have been used in food science as stabilizers and encapsulating agents, in nanoscience as scaffolds to build nanofibers and nanowires, and in drug delivery to form nanoparticles and create helical micelles encapsulating polynucleotides. 1,3-β-Glucans are beginning to have enormous significance due to their dual nature as structure-forming agents and pharmacological substances, and research is especially focused on the application of these polymers in animal nutrition and drug delivery.     

Effect of wetting and drying on DTPA‐extractable Fe,Zn, Mn,and Cu in soils

Abstract

The study reported herein was intended to determine the effect of (i) wet‐incubation and subsequent air‐drying, and (ii) oven‐drying on DTPA‐Fe, Zn, Mn, and Cu.

Analysis of wet‐incubated soils showed significant decreases in DTPA‐Fe, Mn, and Cu at the 1% and Zn at the 10% level of probability. Air‐drying of these moist‐incubated soils increased the levels of Fe, Zn, and Cu to values close to their original levels. Levels of Mn sharply deviated from their original values after air‐drying of incubated soils. Correlation coefficients (r) between the amounts of extractable nutrients in original air‐dry soils and wet‐incubated soils were 0.54, 0.87, 0.91, and 0.13 for Fe, Zn, Cu, and Mn, respectively. Oven‐drying increased the levels of DTPA‐extractable micronutrients from 2 to 6 fold.     

Nitrogen,phosphorus, and potassium effects on pre‐ and post‐transplant growth of salvia and vinca seedlings

Pre‐ and post‐transplant growth of bedding plants is affected by seedling nutrition. However, there is little information available on how seedling nutrition affects the growth of ornamental bedding plants. In this study, we quantified the effects of nitrogen (N) (8 to 32 mM) and phosphorus (P) and potassium (K) concentration (0.25 to 1 mM) of the seedling fertilizer on pre‐ and post‐transplant growth and nutrient element content of salvia (Salvia splendens F. Sellow ex Roem. & Schult.) and vinca (Catharanthus roseus L.) seedlings. Shoot growth of salvia and vinca increased with increasing concentrations of N in the pre‐transplant fertilizer and these differences lasted until the end of the study at 15 days after transplanting. Pre‐transplant root dry mass of these species was not affected by the N concentration of the fertilizer, but root dry mass at 12 days after transplanting was positively correlated with the N concentration of the pre‐transplant fertilizer. Increasing N concentrations in the seedling fertilizer increased tissue N levels of salvia and decreased tissue K level of vinca at transplanting. Increasing P and K concentrations in the pre‐transplant fertilizer increased tissue P level of salvia and P and K levels of vinca, but had little effect on seedling growth. Leaf area and root dry mass at transplanting decreased slightly with increasing P and K concentration in the fertilizer. There were no lasting effects of pre‐transplant P and K concentration of the fertilizer. These results indicate that salvia and vinca seedlings can benefit from high concentrations of N (up to 32 mM) in the fertilizer, while only low concentrations of P and K (0.25 mM) are needed.     

Distribution of Total and DTPA‐Extractable Zinc,Copper, Manganese,and Iron in Vertisols of India

Abstract

Vertisols of India are developed over isohyets of 600 to 1500 mm, and their chemical cycles are set by drainage, landforms, and particle size, which results in variable pedogenic development within the otherwise homogeneous soils. The purpose of this study was to identify pedogenic processes in the distribution of total and DTPA‐extractable zinc (Zn), copper (Cu), manganese (Mn), and iron (Fe). The soils are developed over basaltic parent material of Cretaceous age. Soil samples were drawn from genetic horizons of the 13 benchmark profiles and analyzed by using HF–HClO₄ acid for total and DTPA extraction. Correlation coefficients were calculated taking all samples together. The total concentration varied from 24 to 102 mg kg^?1 for Zn, 21 to 148 mg kg^?1 for Cu, 387 to 1396 mg kg^?1 for Mn, and 2.36 to 9.50% for Fe. Their variability was proisotropic and haplodized, and their concentrations increased with advancing isohyets. Within the isohyets, hindrance in drainage caused retention of Zn and Cu but loss of Fe. The piedmont soils had more Fe than alluvium soils. The spatial distribution of total contents of Zn, Cu, and Fe was influenced by the pedogenic processes associated with Haplusterts but not with provenance materials. Surface concentrations of the elements by biotic lifting and/or harvest removal were negated by the pedoturbation that further contributed to the irregular distribution of the elements in the profiles. Total Zn and total Cu had positive coefficients of correlations with coarse clay, whereas total Mn and total Fe were positively correlated with fine clay. The DTPA‐extractable forms were functions of isohyets and drainage and showed association with organic carbon content and coarse clay.     

Comparison of broiler litter and inorganic nitrogen,phosphorus, and potassium for double‐cropped sweet corn and broccoli

Two rates of broiler litter (20 and 40 mt/ha) were compared to recommended rates of inorganic nitrogen (N), phosphorus (P), and potassium (K) in a double cropping system of spring sweet corn (Zea mays L. ‘Silverqueen') and fall broccoli (Brassica oleracea L, ‘Southern Comet')‐ Sweet corn matured one week earlier both years when fertilized with 40 mt/ha of broiler litter compared to commercial fertilizer. The early maturity may be due to improved P nutrition. Similar or higher yields of fall broccoli were produced with broiler litter following sweet corn than with commercial fertilizer.