Regulation of zinc and manganese transport into developing wheat grains having different zinc and manganese concentrations

Grains of wheat were produced with differing zinc (Zn) or manganese (Mn) contents by culturing detached ears from anthesis onwards in solutions of four different concentrations of Zn or Mn (0.1,1.0,10, and 50 μM). After 20 days, ears were labeled with ⁶⁵Zn or ⁵⁴Mn at (i) the pretreatment concentrations of Zn or Mn, or (ii) at 10 μM Zn or Mn regardless of the pretreatment. Accumulation of Zn or Mn in the grain was greater as the pretreatment concentration of Zn or Mn increased from 1.0 to 10 μM, however, accumulation was less in ears cultured at 50 μM Zn or Mn. Accumulation of Mn in grain of different Mn status labeled at 10 μM Mn was similar in the 0.1,1.0, and 10 μM Mn pretreatments, but accumulation in the grain pretreated at 50 μM Mn was reduced. In contrast, accumulation of Zn in grains of different Zn status when labeled at 10 uM Zn was highest in ears pretreated at 10 μM Zn, but substantially lower in ears of a lower Zn status (those pretreated at 0.1 or 1.0 μM Zn) as well as in those pretreated at 50 μM Zn. These results suggest that Zn‐deficient grain was not a strong sink for Zn, while at high concentrations of solution Zn, a protective barrier exists preventing excessive accumulation of Zn in the grain. Proportionally more Zn was distributed to the inner pericarp and generally less to the endosperm, outer pericarp, and embryo as the Zn status of the grain increased. This work demonstrates that loading of Zn and Mn into, and distribution within, wheat grain is regulated by the nutritional status of the grain.     

Uptake of iron,zinc, manganese,and copper by seedlings of hybrid and traditional rice cultivars from different soil types 1

In pot experiments, uptake of zinc (Zn), copper (Cu), iron (Fe), and manganese (Mn) by hybrid rice from different soil types was compared with a traditional rice (Oryza sativa L.) cultivar. The concentration and total uptake of Fe in the shoots of hybrid rice grown in Oxisol and Ultisol were lower than those of the traditional cultivar. The concentration and total uptake of Zn in the shoots of hybrid rice grown in the Inceptisol (calcareous) were significantly higher than those of the traditional cultivar. Higher ratios of Zn and Fe in upper leaves (UL) to the lower leaves (LL) were found in hybrid rice grown in the calcareous Zn‐deficiency soil. The results indicated that hybrid rice root avoided absorbing excess Fe from Fe‐toxic soils due to its higher oxidizing power, and was more efficient in absorbing Zn from calcareous Zn‐deficient soils than the traditional cultivar.     

分光光度法测定有机肥料中铜、铁、锌、锰含量

探索用分光光度法测定有机肥料中的微量元素铜(Cu)、铁(Fe)、锌(Zn)、锰(Mn)含量,并研究了显色反应的酸度条件、干扰因素及消除方法。该检测方法简单快速,可应用于有机肥生产企业中微量元素铜、铁、锌、锰的测定。     

Assessment of concentrations of iron and zinc and bioavailable iron in grains of early-maturing tropical maize varieties

Twenty elite early-maturing (75-90 days) tropical maize varieties grown in three diverse agroecologies in West Africa were evaluated to identify varieties with high kernel-Fe and -Zn and bioavailable Fe levels. Bioavailable iron was assessed using an in vitro digestion/Caco-2 cell model. Significant (P < 0.001) varietal differences were observed in mean kernel-Fe and -Zn levels. The ranges were 15.5-19.1 mg kg(-)(1) for Fe and 16.5-20.5 mg kg(-)(1) for Zn. Genetic component accounted for 34% of the total variation in kernel-Zn and for 11% of the variation in kernel-Fe levels. Mean bioavailable Fe in varieties ranged between 4% below and 49% above the reference control variety. A significant negative relationship was detected between kernel-P concentration and bioavailable Fe (R = -0.36; P < 0.004; n = 60). Two varieties, ACR90POOL16-DT and ACR86TZESR-W, were identified as the most promising for further evaluation to determine their efficacy as improved sources of iron in target populations.     

Tillage,lime, and poultry litter effects on plant concentrations of zinc,manganese, and copper

Minimum tillage cropping systems and the use of animal manures on cropland are becoming more prevalent. An experiment was initiated to determine the effects of tillage and lime/gypsum variables on uptake of zinc (Zn), manganese (Mn), and copper (Cu) by corn (Zea mays L.) and to show correlations between plant uptake of these metals and soil pH and Mehlich 1‐extractable soil metals where poultry litter was used as a nitrogen (N) source. Surface soil samples were taken in the spring and fall for two years from a long‐term tillage experiment that had been in place for nine years. There were two tillage treatments [conventional (CT) and no‐tillage (NT)] and six lime/gypsum treatments (control, 8,960 kg gypsum ha^‐1 every fourth year, 4,480 kg lime ha^‐1 every fourth year, and three treatments of 8,960 kg lime ha^‐1 in a four‐year period divided by application times into 1, 2, and 4 treatments). Poultry litter was applied each year of the two‐year experiment at a rate of 8.96 Mg ha^‐1 on a dry weight basis. Soil samples were analyzed for pH and Mehlich 1‐extractable Zn, Mn, and Cu, and plant tissue (small plant, ear leaf, stalk, and grain) was analyzed for Zn, Mn, and Cu concentrations. Lime treatments resulted in lower Zn in the small plant and ear leaf for CT, but not for NT. Plant Mn was decreased by lime and gypsum rates for small plant, ear leaf, stalk and grain for both years for CT and NT. Correlations for plant Zn versus soil pH were generally non‐significant, except for one year for ear leaf Zn (R=‐0.413**). Correlations for soil pH and plant tissue Cu were all nonsignificant. Correlations for plant Mn and soil pH were strong with R values over 0.80. Plant Mn response to treatments was found at a pH range of 4.2 to 5.8 for ear leaf and pH 5.2 to 6.2 for stalks. Plant Mn and Zn versus Mehlich 1‐extractable soil Mn and Zn, respectively, were negative. This response was possibly due to oxidation‐reduction and non‐incorporation of the lime for Mn and non‐incorporation of the lime for Zn. Also, the poultry litter was high in Zn (447 mg kg^‐1), which could have masked pH effects. It was concluded that soil sampling for plant micronutrients for NT, especially where a waste material high in micronutrients is applied, can give erratic and even erroneous results. However, lime and tillage treatments had a predictable effect on micronutrient uptake as related to soil pH.     

Extraction kinetics of copper,zinc, iron,and manganese from contaminated sediment using Disodium Ethylenediaminetetraacetate

One technique for cleansing heavy metal contaminated soils is to wash the excavated soil with an extraction solution of a chelating agent. The rate of extraction is an important parameter when considering the length of time needed for soil clean-up and the amount and concentration of wash solution required. The extraction kinetics of copper, zinc, iron and manganese from a contaminated sediment of the Clark Fork River in western Montana, U.S.A., with Disodiun Ethylenediaminetetraacetate (Na₂EDTA) as the extraction agent, were investigated. The results showed the extraction process consisted of rapid extraction in the first minutes followed by much slower extraction for the remainder of the experiment. The rate of extraction, particularly in the rapid phase, demonstrated clear pH dependence: the lower the pH, the faster the extraction rate. In the EDTA concentration range of 0.01 M to 0.05 M, the effect of the EDTA concentration on the extraction rate was not important compared with that of the solution pH. Extraction kinetics for different size particles were similar, although in the first few minutes, EDTA extracted more metals from clay and silt than sand. The two reaction, diffusion, and two-constant kinetic models were compared to experimental results. The two reaction model did not fit any of the data well, and only iron extraction could be described with a simple diffusion model. In general the extraction rates can be well described by the two-constant model, C=A t ^B, up to 600 minutes and under different conditions such as solution pH, EDTA concentration, and different sediment particle size.     

土壤锌、铁、铜、锰形态的分布及其与植物有效性的关系

The distribution of various fractions of Zn,Fe,Cu and Mn in 15 types of soils in China and its relationship with plant availability were studied.Fractions of various elements were found to have some similar characterstic distribution regularities in various types of soils,but various soil types differed to varying degrees in the distribution of each fraction.Soil physico-chemical properties,such as pH,CEC and the contents of OM,CaCO3,free Fe,free Mn and P2O5,were significantly correlated with the distribution of elemental fractions,and a significant correlation also existed between the distribution and plant availability of elemental fractions.Various fractions of each element were divided into two groups based on their plant availability.The correlation between the distribution of combination fractions and plat availability indicated a significantly or an extremely significantly positive correlation for Group I but a significantly or an extremely significantly negative correlation for Group II.Therefore,the fractions in Goup I were primary pools of available nutrients,while those in Group II could hardly provide available nutrients for plants.Decreasing the transformation of corresponding elements into fractions of Group II and increasing the storage capacity of various fractions of Group I were an important direction for regulation and controlling of soil nutrients.However,some Particular soils with too high contents of Zn,Fe,Cu and Mn should be regulated and controlled adversely.     

Long-term effects of tillage on the availability of iron, copper, manganese, and zinc in a Spanish Vertisol

Organic matter accumulation and increased microbial activity under no-till can affect the dynamics of some essential micronutrients for plants. The main purpose of this work was to study the long-term effect of tillage on the availability of Fe, Mn, Cu, and Zn in a calcareous soil from Southern Spain. To this end, nutrient availability in surface soil (0–5-cm depth) subjected to a long-term tillage experiment (21 years) was evaluated via pot experiments and chemical tests involving DTPA extraction (as availability index) and sequential chemical fractionation of Mn and Fe.Soil organic matter (SOM) content and microbial activity (estimated by the β-glucosidase method) were found to be significantly higher under no-till (NT) than under conventional (CT) or minimum tillage (MT). Also, DTPA extractable Mn, Cu, and Zn, and citrate–bicarbonate extractable Mn (Mn_cb), were all higher under NT than under CT and MT, the differences being related to the increase in SOM as revealed by the correlation of Mn, Cu, and Zn extractable with DTPA and SOM (r = 0.87, P < 0.001; r = 0.8, P < 0.01, and r = 0.86, P < 0.001, respectively), and that between Mn_cb and SOM (r = 0.87, P < 0.001). However, the increased extractability resulted in no increased concentrations of these nutrients in plants. Moreover, the Mn concentration in the last expanded leaf was significantly lower with NT than with CT, which can be ascribed at least partly to an increased microbial activity under NT as revealed by the negative correlation between Mn in plants and β-glucosidase activity in soil (r = −0.71, P < 0.01). The Fe concentration in plants was not affected by soil tillage; also, it was only related to citrate–ascorbate extractable Fe (r = 0.69, P < 0.05), which exposes the contribution of poorly crystalline Fe oxides in soil to Fe nutrition in plants.     

Phytotoxicity and some interactions of the essential trace metals iron,manganese, molybdenum,zinc, copper,and boron

Abstract

The essential trace elements Fe, Mn, Zn, Cu, and B in high concentrations can produce phytotoxicities. Iron toxicity resulted from 5 × 10^‐4 M and 10^‐3 M FeSO₄, but not from equivalent amounts of FeEDDHA (ferric ethylenediamine di (o‐hydroxyphenylacetic acid) ). Leaf concentrations in bush beans of 465 μg Mn/g, 291 μg B/g, and 321 μg Zn/g all on the dry weight basis resulted in 27%, 45%, and 34% reduction in yields of leaves, respectively. Zinc was concentrated in roots while Mn and B concentrated in leaves. Solution concentrations of MnS0₄ of 10^‐3 and 10^‐2 M depressed leaf yields of bush beans by 63% and 83%, respectively, with 5140 and 10780 μg Mn/g dry weight of leaves. Copper concentrations were simultaneously increased and those of Ca were decreased. Bush bean plants grown in Yolo loam soil with 200 μg Cu/g soil had a depression in leaf yield of 26% (with 28. 8 μg Cu/g leaf); plants failed to grow with 500 μg Cu/g soil. A level of 10^‐3 M H₂MoO₄ was toxic to bush beans grown in solution culture. Leaves, stems, and roots, respectively, contained 710, and 1054, and 5920 μg Mo/g dry weight.     

Coated urea enhances iron and zinc concentrations in rice grain under different cultivation methods

In order to assess changes of iron (Fe) and zinc (Zn) in rice grain as affected by polyester resin coated urea (PCU) under conventional flooding cultivation (CFC) and plastic film mulching cultivation (PFMC), using prilled urea (PU) as a contrast, a three year field experiment was conducted in Central Zhejiang Province, E China, from 2008 to 2010. The mean annual Fe concentration under PFMC in brown rice was increased by 9.3%, 9.0% and 16.2%; Zn was improved by 21.8%, 16.9% and 12.9%, during 2008, 2009 and 2010, respectively. Compared PCU to PU, Fe concentration in brown rice was improved by 7.3%, 10.1% and 17.9%; Zn concentration in brown rice was improved by 14.8%, 11.3% and 8.1%, during 2008, 2009 and 2010, respectively. Non-flooded PFMC decreased soil organic matter, soil nitrogen (N) and potassium (K) compared to CFC, which suggested that long term PFMC will make soil quality maintenance difficult.     

干旱条件下氮、磷水平对土壤锌、铜、锰、铁有效性的影响

研究了干旱条件下不同N、P水平对微量元素Zn、Cu、Mn、Fe有效性的影响。结果表明 ,在中壤质土壤含水量 11%～ 14%的情况下 ,不同N、P配比明显增加了土壤剖面中速效P的含量 ,但随着土壤深度的增加 ,土壤速效P呈不断下降的趋势。大田和土壤培养试验表明 ,施P有增加土壤Zn、Cu、Mn有效性的趋势 ,但有效Fe则有下降的趋势。土壤速效P、Zn、Cu、Mn、Fe之间的相关分析表明 ,土壤速效P与有效Zn之间存在显著的正相关关系 (r =0.65) ,而与有效Fe之间则存在显著的负相关关系 (r =- 0.63)。     

Effects of sulfur,zinc, iron,copper, manganese,and boron applications on sunflower yield and plant nutrient concentration

Abstract

Sulfur, zinc, iron, copper, manganese, and boron application did not affect the seed yield or oil percentage of sunflower (Hilianthus annuus L.) on both dryland and irrigated soils in North Dakota in 1981. Field averages indicated significant Zn, Mn, and B uptake by sunflower at the 12‐leaf stage as a result of fertilization with these elements. Increased Zn uptake was also observed in the uppermost mature leaf at anthesis from zinc fertilization.

Although sunflower yield from boron fertilization was not significantly different from the check, a trend was observed in which boron fertilization seemed to decrease sunflower yield. Sunflower yields from the boron treatment were the lowest out of seven treatments in three out of four fields. Also, sunflower yield from the boron treatment was significantly lower than both iron and sulfur treatments when all fields were combined.     

Effect of litters on the mobility of zinc, copper, manganese, and iron in the upper horizons of podzolic soils

The concentrations of water-soluble Cu, Zn, Mn, and Fe in the litters of the peaty-podzolic-gleyic and pale-podzolic soils of the Central Forest State Biosphere Reserve are inversely proportional to the pH and directly proportional to the total titratable acidity of the extracts and the content of the water-soluble organic matter (WSOM). During the interaction of the litter solutions with the mineral soil horizons, the content of metals in the solutions can vary depending on the pH, the WSOM concentration in the input solutions, the WSOM amphiphility, and the nature of the metal. The amount of water-soluble Mn, Cu, and Zn in the Eih horizon of peaty-podzolic-gleyic soils is directly proportional to the content of WSOM and inversely proportional to the pH of the litter solutions; the Fe concentration increases parallel to both parameters.     

Effects of moist storage and different drying temperatures on the extractability of iron,copper, manganese,and zinc in soil samples

Abstract

The objective of this study was to investigate the effect of different pretreatments on the extraction of cationic micronutrients [iron (Fe), copper (Cu), manganese (Mn), and zinc (Zn)] from four different soils. Samples were either stored in the field‐moist state for seven days before drying or dried immediately. Three drying treatments were used: air‐dried (72 hours), oven‐dried at 40°C (48 hours), or oven‐dried at 105°C (24 hours). Micronutrients were then extracted with 0.1N HC1 or diethenetriamine‐pentacetic acid (DTPA). Storage, drying temperature, extractant, and soil type all influenced micronutrient extractability. In general, a higher temperature increased the level of micronutrient extracted. However, the reverse effect was also observed. The effect of storage was variable and probably depended on the sample moisture content. We conclude that the results from routine analysis and experimentally determined indices can only be compared if soil samples are subjected to the same pretreatments. Hence, rigorous standardization of the sample preparations is imperative for accurate determination of plant‐available micronutrients.     

Effects of solid phase from pig slurry on iron,copper, zinc,and manganese content of soil and wheat plants

A two‐year lysimeter experiment was conducted using winter wheat plants on two texturally contrasting soils (soil A and soil B). The main objective of this study was to evaluate the influence of increasing doses (5, 10, 15, 20, and 251 ha^‐1) of solid phase from pig slurry (SP) on soil extractable copper (Cu), zinc (Zn), iron (Fe), and manganese (Mn) and on wheat micronutrients composition and uptake. As the control, a basic dressing of NPK fertilizer was applied. Results showed that increasing additions of SP significantly enhanced extractable Cu, Zn, Fe, and Mn content on the topsoil for both soils tested. In addition, a significant increase was detected for extractable Cu, Zn, and Mn content with increasing application rates of SP for subsoil A, but no significant differences were detected for subsoil B. A significant increase in the contents of Fe, Mn, and Zn in the plants as well as total uptakes were observed from increasing doses of SP. Copper content in the plants was not significantly affected. Finally, a strong pH effect was exerted in the Mn and Zn uptake by the plants.     

Interaction between manganese and zinc in growth of rice plants

Many instances of antagonistic relationships among various micro-elements and between micro- and macro-elements in their absorption, translocation, and functions in plant growth have been reported (1). In a series of our researches on the effect of micro-elements on the growth of rice plants, it was observed that manganese toxicity symptom of rice plants was markedly reduced by increasing the concentration of zinc in the nutrient sdution. Although it has been reported that manganese toxicity symptoms of crops were reduced by addition of Fe (2,3), A1 (4), Ca (5) and silicate (6) to the medium, there have been few reports of the effect of Zn on Mn toxicity of rice plants. In this paper, the interaction between Mn and Zn in the growth of rice plants was reported.     

Comparative physiological study of iron,manganese and copper absorption by plants

In the previous reports, influence of metabolic inhibitors, such as NaCN, 2,4-dinitrophenol and EDTA, on the absorption of ⁵⁹Fe, ⁵⁴Mn and ⁶⁴Cu(1) and the distribution of these elements in root cell were studied using the separation technique by centrifugal forces (2). These experiments revealed that rice and barley plants behave differently in their mode of absorption and distribution of these elements.     

The influence of soil properties,total copper,iron, manganese,and zinc on the yield of oat,carrot, onion and lettuce

Abstract

As part of a series of studies for evolving useful soil tests for Cu, Fe, Mn and Zn in cultivated organic soils, 55 such soils from eastern Canada were used in a greenhouse study. Oat, carrot, onion, and all three followed by lettuce were grown in a greenhouse in 2 L pots containing 1.8 L of soil. The moisture, fertilization and temperature conditions provided were known to be non‐Hmiting. And yet, yields of crops, on per kg soil basis, generally correlated negatively with certain soil properties. The more decomposed and denser soils tended to be less productive, even though more soil, but not significantly less water, was accessible to the roots in such soils than in the case of less decomposed, more open soils. In contrast, crop yield per unit soil volume (1.8 L) basis showed a negative effect of water holding capacity on yield of oat, and positive effect of pH on carrot yield. No other correlations were significant. The results thus corroborated the practice of basing soil tests and fertilizer reconmendations on volume rather than weight of organic soils; indicated that the quality of organic soils, in terms of ability to store and cycle water, oxygen and nutrients, declines with length of cultivation, even before nutritional and water management problems arise, probably due to poor soil aeration; and suggested that the effect of soil quality on potential productivity be examined to derive factors that would help refine fertilizer recommendations.     

Tillage,lime, and poultry litter effects on soil zinc,manganese, and copper

Abstract

Long‐term no‐tillage has profound effects on soil properties which can affect the availability of plant nutrient elements. The objectives were to study the effects of tillage and lime treatments on soil pH and extractable soil micronutrients where poultry litter was used as a nitrogen (N) source. Surface soil samples were taken in the spring and fall for two years from a long‐term tillage experiment that had been in place for nine years. There were two tillage treatments [conventional (CT) and no‐tillage (NT)] and six lime/ gypsum treatments (control, 8,960 kg gypsum ha^‐1 every fourth year, 4,480 kg lime ha^‐1 every fourth year, and three treatments of 8,960 kg lime ha^‐1 in a four‐year period divided by application times into 1, 2, and 4 treatments per year). Poultry litter was applied each year of the two‐year experiment at a rate of 8.96 Mg ha^‐1 on a dry weight basis. The crop was corn (Zea maize L.). Soil samples were analyzed for pH and Mehlich‐1 zinc (Zn), manganese (Mn), and copper (Cu). Soil pH was higher for NT than CT and was higher in the spring than in the fall. Lime rates resulted in soil pH increases, but showed less difference for CT than NT. The three 8,960 kg ha^‐1 per four yr treatments caused an interaction in that for CT the pH increased more for 2,240 kg ha^‐1each year than for 8,960 kg ha^‐1 every fourth year and the opposite was true for NT. Extractable Zn, Mn, and Cu all responded to this interaction being lower for the higher pH plots. Extractable Zn was higher for NT possibly due to high Zn from the poultry litter and non‐incorporation for NT. Extractable Cu was lower for NT as expected from the soil pH, whereas extractable Mn was not affected by tillage. Extractable Zn and Cu both increased over time due to inputs from the poultry litter. Neither extractable Zn nor Mn responded to increasing lime rates, however Cu decreased with increasing lime rate. Extractable Cu was influenced mainly by soil pH differences due to tillage and lime. Extractable Zn was influenced much more by tillage and from inputs by the poultry litter and not as much by pH differences. Extractable Mn was the least responsive to tillage and lime treatments of the three micronutrients studied.     

不同的水稻品种产量及生理氮素利用效率的差异

Efficient use of N in agricultural practice can increase yield, decrease production costs and reduce the risk of environmental pollution. Effects of N fertilizer application rates on grain yield and physiological N use efficiency (PE) in relation to the accumulation and redistribution of biomass and N in rice (Oryza sativa L.) cultivars were studied at two experimental farms of Nanjing Agricultural University, Nanjing, China in 2004. Three high N use efficiency (NUE) rice cultivars (Wuyunjing 7, Nanguang and 4007) and one low NUE rice cultivar (Elio) with similar growth patterns were studied under seven N rates (0, 60, 120, 180, 240, 300 and 360 kg ha^-1). Grain yield increased with the N application rate and attained plateau at 180 kg N ha^-1 for rice cultivars at each site. Increasing N rate decreased PE for biomass and grain yield. Grain yield and PE of Elio were about 20% and 18% lower than those of high NUE cultivars. Differences in biomass, N accumulation and N redistribution were observed at the post-heading stage among rice cultivars with differing NUEs. The less reproductive tillers of Elio resulted in less demand for C and N during grain filling, thus leading to lower PE of Elio compared with the high NUE rice cultivars.