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1.
Mitsuhiro KAMEYAMA Kiyoshi TOMINAGA Junko YABATA Yasuharu NOMURA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(11):1437-1441
Patterns of insertion sequence (IS)629, norV
genotype, and Shiga toxin (Stx) genotype distribution were investigated amongst 203
enterohemorrhagic Escherichia coli O157 isolates collected in Yamaguchi
Prefecture, Japan, between 2004 and 2013. A total of 114 IS629 patterns
were identified; these were divided into eight IS groups (A–H). Ninety isolates carried an
intact norV gene, whereas 113 isolates carried a norV
with a 204-bp deletion. Other than one isolate from IS group G, all isolates with an
intact norV belonged to groups A–F, whereas isolates with a mutant
norV belonged to IS groups G and H. Seven stx
genotypes were identified, and of those, stx1a/stx2a was
predominant (n=105), followed by stx2c (n=32) and stx2a
(n=27). The stx1a/stx2a genotype was associated with the
mutant norV isolates, whereas isolates with an intact
norV had the stx2c genotype. Therefore, certain
combinations of IS type and stx genotype appear to be more frequent among
O157 clades which may be useful for detection of predominant subtypes in the interest of
public health. 相似文献
2.
Pramod DHAKAL Nobuo TSUNODA Rie NAKAI Kentaro NAGAOKA Yasuo NAMBO Fumio SATO Hiroyuki TANIYAMA Watanabe Kazuyoshi TAYA 《Journal of Equine Science》2011,22(1):9-15
The aim of present study was to clarify the post-natal profile of follicle-stimulating
hormone (FSH), luteinizing hormone (LH), immunoreactive (ir)-inhibin, progesterone,
testosterone, and estradiol-17β, and their relationships in Thoroughbred colts. Six
hundred and thirty-six colts were used for the study. Single plasma samples from each
animal were harvested from the blood drawn through jugular venipuncture. The subjects were
born with high amounts of progesterone, testosterone, and estradiol-17β, all of which
dropped significantly and remained at lower levels till the end of 6 months. FSH decreased
transiently after birth until day 12 and then gradually increased to peak at day 100 which
then maintained in lesser levels towards the end of the studied period. LH was highest
during birth which decreased until day 26 and then increased slowly to sub-birth levels up
to day 90. Animals were born with high amounts of ir-inhibin. It dropped slowly and halved
by day 20 and then decreased towards rest of the studied period. The increase in FSH is
negatively correlated with the declining ir-inhibin levels. The early increase in FSH can
be the indication of early post-natal maturation of the hypothalamic pituitary testicular
axis that ultimately might be responsible for priming the testes for future
development. 相似文献
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Joanna PIASECKA-SRADER Agnieszka SADOWSKA Anna NYNCA Karina ORLOWSKA Monika JABLONSKA Olga JABLONSKA Brian K. PETROFF Renata E. CIERESZKO 《The Journal of reproduction and development》2016,62(1):103-113
Low doses of endocrine disrupting chemicals (EDCs) used in combination may act in a manner different from
that of individual compounds. The objective of the study was to examine in vitro effects of
low doses of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD; 100 pM) and genistein (500 nM) on: 1)
progesterone (P4) and estradiol (E2) secretion (48 h); 2) dynamic changes in aryl
hydrocarbon receptor (AhR) mRNA and protein expression (1, 3, 6, 24 and 48 h); 3) dynamic changes in estrogen
receptor β (ERβ) mRNA and protein expression (1, 3, 6, 24 and 48 h); and 4) induction of apoptosis in porcine
granulosa cells derived from medium follicles (3, 6 and 24 h). TCDD had no effect on P4 or
E2 production, but potentiated the inhibitory effect of genistein on P4 production. In
contrast to the individual treatments which did not produce any effects, TCDD and genistein administered
together decreased ERβ and AhR protein expression in granulosa cells. Moreover, the inhibitory effect of TCDD
on AhR mRNA expression was abolished by genistein. The treatments did not induce apoptosis in the cells. In
summary, combined effects of low concentrations of TCDD and genistein on follicular function of pigs differed
from that of individual compounds. The results presented in the current paper clearly indicate that effects
exerted by low doses of EDCs applied in combination must be taken into consideration when studying potential
risk effects of EDCs on biological processes. 相似文献
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Takashi KOZASA Yuri ABE Kazuya MITSUHASHI Tomokazu TAMURA Hiroshi AOKI Masatoshi ISHIMARU Shigeyuki NAKAMURA Masatoshi OKAMATSU Hiroshi KIDA Yoshihiro SAKODA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(5):511-518
The Exaltation of Newcastle disease virus (END) phenomenon is induced by the
inhibition of type I interferon in pestivirus-infected cells in vitro,
via proteasomal degradation of cellular interferon regulatory factor (IRF)-3 with the
property of the viral autoprotease protein Npro. Reportedly, the amino acid
residues in the zinc-binding TRASH motif of Npro determine the difference in
characteristics between END-phenomenon-positive (END+) and
END-phenomenon-negative (END−) classical swine fever viruses (CSFVs). However,
the basic mechanism underlying this function in bovine viral diarrhea virus (BVDV) has not
been elucidated from the genomic differences between END+ and END−
viruses using reverse genetics till date. In the present study, comparison of complete
genome sequences of a pair of END+ and END− viruses isolated from
the same virus stock revealed that there were only four amino acid substitutions (D136G,
I2623V, D3148G and D3502Y) between two viruses. Based on these differences, viruses with
and without mutations at these positions were generated using reverse genetics. The END
assay, measurements of induced type I interferon and IRF-3 detection in cells infected
with these viruses revealed that the aspartic acid at position 136 in the zinc-binding
TRASH motif of Npro was required to inhibit the production of type I interferon
via the degradation of cellular IRF-3, consistently with CSFV. 相似文献
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Jozsef RáTKY Istvan EGERSZEGI Peter TOTH Soukanh KEONUCHAN Takashi NAGAI Kazuhiro KIKUCHI Noboru MANABE Klaus-Peter BRüSSOW 《The Journal of reproduction and development》2013,59(5):437-441
Worldwide, only a few “fatty” pig breeds exist with different and/or regional
utilization. Using the Hungarian Mangalica, which almost went extinct in Europe and the
Lao Moo Lat pig, which still has a large population in South-East Asia as exemples, we
wanted to demonstrate that indigenous (fatty) pig breeds may represent both national value
and tremendous economic potential. Since these less prolific and less productive breeds
cannot contribute to mass production, new market roles and methods should be established
for them in the premium segment of pork trading. Thus their preservation and propagation
needs the comprehensive collaboration of commercial, governmental actors and researchers.
Briefly summarizing the history, we report the current results of reproductive physiology
research. The commercial renaissance of Mangalica pigs is indebted to the enthusiastic
efforts of basic scientists, pig breeding experts and dedicated Mangalica producers.
Scientific achievements were applied to practical breeding and production of delicious
pork and processed products, which ultimately made the economic success in the Mangalica
sector possible. Both, research on and utilization of endangered (pig) breeds maintain not
only breed diversities, but also may improve the livelihood of farmers worldwide. 相似文献
11.
Md. Masudur RAHMAN Erdenebelig UYANGAA Young Woo HAN Jin HUR Sang-Youel PARK John Hwa LEE Koanhoi KIM Seong Kug EO 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(4):395-403
Newcastle disease (ND) is a highly contagious disease of chickens causing significant
economic losses worldwide. Due to limitations in the efficacy against currently
circulating ND viruses, existing vaccination strategies require improvements, and
incorporating immunomodulatory cytokines with existing vaccines might be a novel approach.
Here, we investigated the systemic and mucosal immunomodulatory properties of oral
co-administration of chicken interleukin-18 (chIL-18) and chicken interferon-α (chIFN-α)
using attenuated Salmonella enterica serovar Typhimurium on an
inactivated ND vaccine. Our results demonstrate that oral administration of S.
enterica serovar Typhimurium expressing chIL-18 or chIFN-α provided enhanced
systemic and mucosal immune responses, as determined by serum hemagglutination inhibition
antibody and NDV Ag-specific IgG as well as NDV Ag-specific IgA in lung and duodenal
lavages of chickens immunized with inactivated ND vaccine via the intramuscular or
intranasal route. Notably, combined oral administration of S. enterica
serovar Typhimurium expressing chIL-18 and chIFN-α significantly enhanced systemic and
mucosal immunity in ND-vaccinated chickens, compared to single administration of
S. enterica serovar Typhimurium expressing chIL-18 or chIFN-α. In
addition, oral co-administration of S. enterica serovar Typhimurium
expressing chIL-18 and chIFN-α provided enhanced NDV Ag-specific proliferation of
peripheral blood mononuclear cells and Th1-biased cell-mediated immunity, compared to
single administration of either construct. Therefore, our results provide valuable insight
into the modulation of systemic and mucosal immunity by incorporation of immunomodulatory
chIL-18 and chIFN-α using Salmonella vaccines into existing ND
vaccines. 相似文献
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Gábor árpád CZIRJáK Lóránd Béla K?B?LKUTI Miklós TENK Attila SZAKáCS Attila KELEMEN Marina SP?NU 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(6):701-703
A natural hybrid of Vipera ammodytes × Vipera berus was
presented having low body weight, seizures and generalized swelling of the cephalic
region. Based on the history of the case and clinical examination, hemorrhagic stomatitis
of traumatic origin was diagnosed. The snake was kept in a terrarium with wood chips as a
substrate, and the material had induced trauma in the oral mucosa which was further
complicated with Salmonella Arizonae and Morganella
morganii co-infection, abscessation and osteomyelitis. To the best of the
authors’ knowledge, this is the first reported case of bacterial infection in European
snake hybrids and one of a few case reports in European snakes. Although wood chips are an
inexpensive substrate, based on our findings, they should be avoided when keeping and
breeding European vipers. 相似文献
14.
采用NaF水溶液进行累代或隔代添食的方法,选择"研137"、"丰一"、"7N"、"湘F"四个元母种,组配成四元杂交组合"研·丰×7N·湘F"进行室内及农村试养,并参加广东省新蚕品种品比鉴定.结果表明,该杂交组合耐氟性能强,综合经济性状优于现行生产品种"9·芙×7·湘". 相似文献
15.
Maria Federica TROMBETTA Pier Attilio ACCORSI Adalberto FALASCHINI 《Journal of Equine Science》2010,21(1):1-6
When the mare’s estrous cycle resumes in winter, the β-carotene content of hay is
depleted. Sixty Italian trotter mares were randomly assigned to a Control or a Treated
Group. Treated Group received 1g/d synthetic β-carotene for 15 days from parturition.
Blood samples collected at parturition and on days 5, 10 and 15 after partum were analysed
for β-carotene, vitamins A, progesterone, 17 β-estradiol, the energy parameters (glucose,
cholesterol, NEFA), the protein profile (total protein, albumin, urea) and LDH. Some
changes in these measures were attributable to treatment, which significantly affected
β-carotene and 17 β-estradiol concentrations. A significant effect was also found on the
resumption of estrous activity (χ2 test=P<0.052). 相似文献
16.
Y. Brito‐Casillas P. Figueirinhas J.C. Wiebe L. Lpez‐Ríos D. Prez‐Barreto C. Melin A.M. Wgner 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2014,28(5):1405-1413
Background
Portable blood glucose meters (PBGMs) allow easy glucose measurements. As animal‐specific PBGMs are not available everywhere, those for humans are widely used.Objectives
To assess the accuracy and precision of 9 PBGMs in canine whole blood (WB) and plasma, based on the ISO 15197:2013.Animals
Fifty‐nine client‐owned dogs attending the Veterinary Teaching Hospital.Methods
Analytical evaluation of 100 blood samples was performed for accuracy and 23 for precision (glucose 29–579 mg/dL) following ISO recommendations. A PBGM was considered accurate if 95% of the measurements were within ±15 mg/dL from the reference when glucose was <100 mg/dL and within ±15% when it was ≥100 mg/dL, and if 99% of them were within zones A and B in error grid analysis (EG). A hexokinase‐based analyzer was used as reference. Ninety samples were assessed for hematocrit interferences.Results
Accuracy requirements were not fulfilled by any PBGM in WB (74% of measurements within the limits for the most accurate) and by 1 only in plasma. However, the EG analysis in WB was passed by 6 PBGM and by all in plasma. The most accurate were also the most precise, with coefficients of variation <5% in WB and <3% in plasma. Hematocrit correlated with bias against the reference method in 4 PBGM (r = −0.243 − [−0.371]; P < .021).Conclusions and Clinical Importance
This disparity among PBGM suggests that meters approved for humans need to be evaluated before use in other species. 相似文献17.
Chunqiang WANG Chunjing LI Hongjiao LI Wei MA Shuxiong CHEN Yun ZHAO Jiahui RAO Xu ZHOU 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(11):1419-1425
Inhibins, as members of the transforming growth factor beta (TGF-β)
superfamily, downregulate the synthesis and secretion of follicle-stimulating hormone
(FSH) in an endocrine manner. The role of inhibin/betaglycan in the ovary regulation
recently gained attention. To date, no data exist on the function of inhibin α subunit and
betaglycan in cystic follicles. In this study, the expressions of inhibin α subunit and
betaglycan in cystic follicles were investigated using immunohistochemistry, real-time PCR
and Western blot analysis. Both inhibin α subunit and betaglycan immunoreactivities were
mainly localized in the granulosa cells of follicles. Expression of inhibin α subunit and
betaglycan was inferior in cystic follicles compared with that in normal large follicles.
However, the result of enzyme-linked immunosorbent assay showed no significant difference
in the decreasing in concentration of inhibin α subunit in cystic follicular fluid
compared with the control (P>0.05). In this study, we explored the
effects of FSH on betaglycan expression in granulosa cells in vitro. As
expected, a significant increase in the expressions of betaglycan mRNA and protein in
granulosa cells was observed in response to exogenous FSH (30
ng/ml) (P<0.05) compared with the
control. Consequently, this study provides evidence that the expressions of inhibin α
subunit and betaglycan are inferior in cystic follicles, and this may be caused by the
decrease in FSH in the presence of a cystic follicle. 相似文献
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Bartosz WOJCIECHOWICZ Genowefa KOTWICA Justyna KOLAKOWSKA Anita FRANCZAK 《The Journal of reproduction and development》2013,59(1):49-58
Steroid hormones are produced by the porcine uterus. We hypothesized that the uterus in
pigs possesses active 3β-hydroxysteroid dehydrogenase/Δ5-Δ4
isomerase (3β-HSD) responsible for progesterone and androstenedione production, that
uterine steroids may supplement the amount of steroid hormones produced by embryos and
corpus luteum and that these steroids are necessary for maintenance of pregnancy. In this
study, we examined 1) endometrial and myometrial expression of 3β-HSD
mRNA, 2) uterine 3β-HSD protein activity and 3) in vitro production of
A4 and P4 by uterine slices harvested from pigs on days 10 to 11,
12 to 13 and 15 to 16 of pregnancy and the estrous cycle. The expression of
3β-HSD and the presence and activity of 3β-HSD protein were different
in the endometrium and the myometrium during the examined periods of pregnancy and the
estrous cycle. Production of A4 by the endometrium and myometrium was highest
on days 12 to 13 of pregnancy and the estrous cycle. Endometrial secretion of
P4 did not differ in the course of early pregnancy and on the respective days
of the estrous cycle. The gravid myometrium was the highest source of P4 in
pregnant pigs on days 12 to 13. The release of P4 by the cyclic myometrium rose
during the examined days of the estrous cycle. The steroidogenic activity of the uterus,
as described in this study, may support early pregnancy or the luteal phase of the estrous
cycle in pigs. 相似文献