防治西葫芦和黄瓜白粉病的生物制剂的筛选

瓜类白粉病是蔬菜上的重要病害之一,为加大对该病害的生物防治力度,本研究采用课题组研发的1×109cfu/g玫瑰黄链霉菌水剂和10亿芽胞/g枯草芽胞杆菌悬浮剂以及市场上常用的枯草芽胞杆菌、哈茨木霉菌、寡雄腐霉、武夷菌素、多抗霉素等多种生物制剂进行田间药效试验,比较几种生物制剂的防治效果。结果表明,供试生物制剂对西葫芦白粉病的防效为57.65%~84.98%,其中3%多抗霉素水剂600倍液的防效最好,为84.98%,且具有明显的促生长作用,增产率达12.65%。其次为1 000亿芽胞/g枯草芽胞杆菌可湿性粉剂(武汉天惠)400倍液、2%武夷菌素水剂600倍液和3×108 cfu/g哈茨木霉菌可湿性粉剂300倍液,可作为西葫芦白粉病防治的选用药剂。1×109 cfu/g玫瑰黄链霉菌水剂对西葫芦和黄瓜白粉病均具有较好的防效,分别为78.68%和73.59%,具有开发和应用的市场价值。     

Incidence and density relationships of powdery mildew on apple

ABSTRACT The relationships between disease incidence and colony density and between leaf and shoot disease incidences for apple powdery mildew were investigated over four seasons in order to derive a simple relationship for predicting density using incidence. The Neyman type A distribution generally provided a good fit to the observed number of colonies per leaf and shoot, and provided a significantly better fit than the Poisson distribution, indicating a degree of aggregation of mildew colonies. In general, Taylor's power-law satisfactorily described the observed variance-mean relationship for colony density; however, Taylor's power-law broke down at very high levels of mean density. Incidence of leaf infection could be determined based on average number of colonies per leaf assuming a fixed variance-mean relationship and a Neyman type A distribution for colony density. Regression models using the complemen- tary log-log transformation of incidence also provided accurate predictions of leaf (or shoot) disease incidence from colonies per leaf (or per shoot). Similar accuracies of these incidence-density models suggested that variance-mean ratio of colony density was more or less constant over time. Unlike the case with colony density, the number of mildewed leaves per shoot generally had a random pattern, as indicated by the good fit of the binomial distribution. Thus, it was possible to estimate the leaf incidence of the youngest unrolled leaves on a shoot using the shoot incidence. It is argued that the leaf incidence-density relationships developed in the present study may be used in making practical disease management decisions.     

Efficiency of triforine and binapacryl in tank mixtures with calcium chloride against apple powdery mildew

The mildew fungicide triforine is incompatible with calcium chloride used to reduce the incidence of bitter pit in apples. Two commercial non-ionic surfactants delayed sedimentation in spray mixtures containing both triforine and calcium chloride. The biological efficiency of triforine, alone or with calcium chloride plus surfactant, was tested in an orchard trial which included binapacryl, alone or with calcium chloride, as ‘standard’ treatment. Triforine (weekly or fortnightly) controlled mildew on Cox's Orange Pippin and Golden Delicious better than binapacryl, and the addition of calcium chloride did not affect adversely the control by either fungicide. The added calcium chloride had little effect on the deposition and persistence of the fungicides. The inclusion of calcium chloride in spray programmes slightly improved the yields (both cultivars) and russet grade (Cox only) associated with each fungicide. Calcium chloride sprays, whether mixed or alternated, increased the calcium levels in postharvest fruit for both varieties, and decreased the incidence of bitter pit for Cox after storage for 5½ months. No adverse effect was associated with the inclusion of the surfactants in tank mixtures containing either fungicide.     

Relating disease progress to cumulative numbers of trapped spores: apple powdery mildew and scab epidemics in sprayed and unsprayed orchard plots

A technique for relating the progress of plant diseases caused by airborne fungal pathogens to cumulative numbers of trapped spores is proposed. The relationship involves two epidemiological parameters—a disease asymptote and the infection efficiency (disease units/spore) of inoculum. The technique was evaluated using data on apple powdery mildew and scab epidemics in sprayed and unsprayed apple orchard plots. For powdery mildew, the observed relationships were close to those proposed in the unsprayed plot, but changed after or during the period of fungicide application in sprayed plots. Parameter estimates gave useful comparative information on the epidemics. The technique was not useful for scab because of the discontinuous patterns of infection.     

Modelling and forecasting epidemics of apple powdery mildew (Podosphaera leucotricha)

A model developed to simulate epidemics of powdery mildew on vegetative shoots of apple generates two types of output. Firstly, it forecasts disease severity (percentage of host tissue infected) by incorporating effects on disease development of the amount of healthy susceptible tissue and current infectious (sporulating) disease, the level of initial inoculum (overwintered 'primary' mildew) and weather conditions. The effects of weather variables are considered on only two aspects of the fungal life cycle: initial spore germination and the subsequent development during the incubation period. Secondly, the model generates indices of the relative favourability of weather conditions on disease development by incorporating effects of weather on conidial production/dispersal and germination. On each day, forecasts of the (relative) severity of new infection and total current infectious disease are given for both types of output. The model was evaluated by comparing its predictions with the mildew epidemics observed in two unsprayed orchards over four years. In all the years, the temporal patterns of the predicted and the observed disease were generally similar. The pattern of the disease severity forecasts was marginally closer to the observed than that derived from two weather indices. Potential roles of the model in practical management of apple powdery mildew are discussed.     

Effects of fungicides on powdery mildew, tree growth and cropping of apple

In a 6-year orchard experiment, seven fungicide programmes were assessed for control of powdery mildew (Podosphaera leucotricha) and for long-term effects on growth and cropping of the apple cultivar Cox's Orange Pippin. Programmes based on binapacryl, bupirimate, fenarimol, nitrothal-isopropyl and triadimefon gave better control than dinocap or triforine. The heaviest cumulative yields of marketable fruit were obtained with the non-systemic fungicides binapacryl and nitrothal-isopropyl, the former significantly outyielding triadimefon and triforine over 6 years. Tree vigour, as assessed by extension shoot length and internode length, was found to be better after 4-5 years of programmes based on binapacryl than on triadimefon or fenarimol. A relation was demonstrated between cumulative marketable yield and mean annual mid-season incidence of secondary mildew on extension shoot leaves.     

Components of resistance of apple to powdery mildew (Podosphaera leucotricha)

Components of resistance of apple to Podosphaera leucotricha were assessed in the glasshouse by spraying shoot tips of cultivars with conidia. Disease incidence (proportion of leaf surfaces with mildew) and colony numbers were higher and incubation periods correspondingly shorter on the older leaves. unrolled at the time of inoculation. Numbers of colonies were higher on the lower surface. Spore production per colony was higher on the younger leaves. In general, a short incubation period on a cultivar was associated with a high disease incidence, many colonies and high spore production. For some cultivars the association between these components permitted resistance to be classified as very high (cv. Discovery), very low (cv. Golden Delicious), or intermediate(cvs Bramley's Seedling. Suntan). The effects of inoculum concentration and post-inoculation environment (glasshouse or outdoors) on components of resistance were assessed in a further study. No interactions between cultivar and inoculum concentration were detected. There was generally an associated variation in components between cultivars within an environment, but inconsistencies in cultivar rankings were noted between the two environments and the two studies. Mean incubation period was shorter and colony area larger in the glasshouse than outdoors. There were significant differences in conidia/cm² colony between cultivars only in the glasshouse. Components conferring high resistance in cv. Discovery and low resistance in cv. Golden Delicious were confirmed in both environments and in both studies.     

Components of spore production in apple powdery mildew (Podosphaera leucotricha)

The morphology of powdery mildew ( Podosphaera leucotricha) colonies of known age was studied on six apple cultivars inoculated in a glasshouse. Colonies were observed first on the older leaves. Mean disease incidence ranged from about 20% for cv. Laxton's Superb to about 45% for cv. Crispin. Conidiophore density (number of conidiophores/mm² colony) was higher on younger than on older leaves, higher on the upper than on the lower leaf surface, and decreased with the age of the colony. The number of hyphal interceptions on a line transect was higher on the upper surface but the ratio of conidiophore density to hyphal interceptions was greater on the lower surface and did not differ with colony age or cultivar. The number of conidia/conidiophore was higher on the younger leaves and on the upper surfaces and was at a maximum 7-12 days after the first sign of the colony. The number of conidia/mm² colony was higher on lower surfaces, especially of the younger leaves. There was generally an associated variation between cultivars in components of spore production.     

白粉寄生孢不同菌株对控制黄瓜和苹果自粉病病菌的试验研究

为了筛选出更好的能控制白粉病病菌的白粉寄生孢生防菌株,将白粉寄生孢不同菌株对黄瓜白粉病病菌和苹果白粉病病菌分别进行了生防测定.结果显示,白粉寄生孢不同菌株对白粉病病菌的寄生效果和控制作用各异.在白粉寄生孢的不同菌株中,AQ40、AQ9、AQ117对控制温室黄瓜白粉病具有较好的效果,而AQGA-22、AQ13、AQB15-20对控制苹果白粉病的效果较好.表明这些菌株对黄瓜白粉病和苹果白粉病具有一定的选择性和较好的生防应用潜力.     

番茄与白粉病菌互作反应中活性氧的积累

番茄白粉病菌Oidium neolycopersici是一种产生单生分生孢子的真菌,主要分布在欧洲、非洲、南北美洲和亚洲的一种世界性植物病害的病原菌~([1]).已有研究表明,受病原菌侵染的植物体内活性氧的积累与植物抗病性密切相关~([2]).目前关于番茄与番茄白粉病菌互作中活性氧进发与抗病性关系的研究,国内外很少报道.     

Biological control mechanisms of D-pinitol against powdery mildew in cucumber

D-pinitol is an effective agent for controlling powdery mildew (Podosphaera xanthii) in cucumber. In this study, we determined the mechanisms of D-pinitol in controlling powdery mildew in cucumber plants. We compared P. xanthii development on cucumber leaf surface treated with D-pinitol or water (2 mg ml⁻¹) at different time points after inoculation. The germinating conidia, hyphae, and conidiophores of the pathogen were severely damaged by D-pinitol at any time of application tested. The highest level of suppression of fungal development was obtained at 3 days after inoculation. The contents of chlorophyll, total phenolics, flavonoid, and gallic acid and its derivatives (GAD); the activities of phenylalanine ammonialyase (PAL), polyphenoloxidase (PPO), peroxidase (POX), and superoxide dismutase (SOD); and the expression of the genes encoding for PR-1, peroxidase (POX), lipoxygenase (LOX1), chitinase (Chit1) were higher in the cucumber leaves treated with D-pinitol and inoculated than in the leaves either treated with D-pinitol or inoculated with the pathogen. These results suggest that D-pinitol triggers several plant defense responses in cucumber leading to pathogen suppression and resistance to powdery mildew.     

Efficacy of biocontrol agents and natural compounds against powdery mildew of zucchini

The activity of different types of natural compounds and of two biofungicides based on Bacillus subtilis and Ampelomyces quisqualis alone and in combination with fungicides was tested against powdery mildew of zucchini. The efficacy was compared to the activity of fungicides used alone in four experimental trials carried out in the open field and under greenhouse conditions. The Podosphaera xanthii population used throughout the work was partially resistant to azoxystrobin, whereas it was susceptible to mychlobutanil. Sulphur plus terpenes and mustard oil consistently controlled powdery mildew, followed by mychlobutanil alone or in combination with A. quisqualis. B. subtilis and A. quisqualis when tested alone were partially effective. The combination of azoxystrobin and B. subtilis only delayed the spread of the pathogen.     

Activity against rust and powdery mildew of somepara phenylenediamines and related compounds

Twenty-fivepara phenylenediamines and related compounds were tested for activity against powdery mildew on cucumber and against rust on bean. Some remarkable changes in activity resulted from minor changes in the chemical structure of the compounds. It is suggested that the fungicidal activity of the phenylenediamines is correlated with the polarographic halfwave potentials. This might indicate that one of the oxidation products, probably the semiquinone ion, is responsible for activity.     

8种植物提取物对小麦白粉病病菌抑制活性研究

采用室内盆栽法,测定8种植物提取物对小麦白粉病的抑菌活性。结果表明,香樟叶、黄杨和刺槐提取物对小麦白粉病病菌(Blumeria graminis f.sp.tritici)有良好的抑制活性。其粗提物干质量浓度为40 mg/m L时,对白粉菌抑菌效果依次为57.68%,38.67%,42.39%;香樟叶粗提物对小麦白粉的室内预防和治疗作用的EC50分别为10.39 mg/m L和30.43 mg/m L,其预防效果显著优于治疗效果;测定香樟叶4种萃取物对小麦白粉病的预防效果,发现石油醚萃取物抑菌效果最好,EC50为8.71 mg/m L。说明其抑菌活性物质主要存在石油醚相中。     

Epidemics of apple powdery mildew (Podosphaera leucotricha) in a mixed cultivar orchard

Epidemics of apple powdery mildew were monitored on leaves of vegetative extension shoots in a mixed-cultivar orchard in 1979. 1980 and 1981. Cultivars Cox's Orange Pippin. Golden Delicious. Ida Red and Suntan were very susceptible but little mildew developed on cv. Discovery in any year. Results with cvs Crispin and Spartan were less consistent due, in part, to differing patterns of vegetative growth. The rankings of cultivars for disease incidence (proportion of leaves with mildew), number of colonies and mildewed area were similar, and were also consistent on fungicide-sprayed and unsprayed trees in 1981. Less disease occurred on fungicide-treated Golden Delicious than on Cox's Orange Pippin. Most infections occurred on the rolled leaves at the shoot tip. Spore production was greatest on leaves at positions -1 to -4 where leaf 0 was the youngest and leaf - 1 the adjacent and older unrolled leaf.     

四氟醚唑对黄瓜的安全性及其对黄瓜白粉病的防治效果

为明确四氟醚唑对黄瓜植株的安全性,采用浸种及茎叶喷雾处理的方法测定其对不同生长时期黄瓜植株的影响,采用子叶保湿培养法测定白粉病菌对其敏感性,并在田间进行防治白粉病的药效试验,综合评价了四氟醚唑对黄瓜白粉病的效果。浸种处理后,黄瓜子叶上白粉病发病率明显降低,对黄瓜株高和根长的抑制率、茎周增长率及叶绿素含量增加率与己唑醇相比均较低;发芽期及幼苗期的黄瓜植株使用四氟醚唑处理,新生节间均出现轻微的抑制伸长现象;4％四氟醚唑水乳剂对黄瓜白粉病菌的EC50为0．8146mg／L,敏感毒力高于50％醚菌酯水分散粒剂;田间间隔期10天喷雾,在末次药后7天对黄瓜白粉病的防治效果为76．02％～85．77％,与5％己唑醇水乳剂防效相当,明显高于50％醚菌酯水分散粒剂的防效。表明四氟醚唑对黄瓜生长安全且用药间隔期长,是防治白粉病的高效轮换药剂。     

Temporal patterns of airborne conidia of Podosphaera leucotricha, causal agent of apple powdery mildew

In each of the five years 1969 and 1971-1974 inclusive a volumetric spore trap was used in an apple orchard to monitor changes in the number of airborne conidia of Podosphaera leucotricha , the causal agent of apple powdery mildew. The number of trapped conidia varied greatly between years. Time-series analyses, using autoregressive integrated moving average (ARIMA) models, revealed that the temporal pattern of the number of airborne conidia was similar in all years, generally following a diurnal pattern with an afternoon peak. A strong correlation between consecutive hourly counts indicated that the number of trapped conidia depended on the strength of sporulating sources. Using the time-series transfer function (TF) method, it was shown that in each year the most important weather variables influencing the number of airborne conidia were vapour pressure deficit (VPD) and rainfall. Variation between years in the dynamic effects of these variables on conidium numbers was detected, and may reflect weather differences between years. Stepwise regression analysis was applied to the combined daily data for 1973 and 1974 using a subset of weather variables as independent variables, chosen on the basis of TF analysis. A resulting regression model accurately predicted the temporal pattern of conidium numbers (expressed as a percentage of the maximum daily number trapped in the same year) in both years. When this model was applied to the other three years there was good agreement between predicted and observed temporal patterns. Application of this regression model for practical disease control is discussed.     

Eradication of apple powdery mildew (Podosphaera leucotricha) with dormant-season sprays of surface-active agents

Of twenty non-ionic surfactant formulations tested in 1974–75 several were successful as eradicants of overwintering apple mildew as were two ionic materials. The incidence of Nectria canker was increased by sprays applied in October but less so by the same materials applied in January or February when phytoxicity measured in terms of fruit yield was also less. PP 222, a non-ionic surfactant introduced in 1975, proved to be an efficient eradicant of mildew in 1976. Although the yield of fruit from most sprayed trees was greater than from controls, the best mildew eradicants did not produce greatest yields in the same year, perhaps indicating adverse effects of treatments on the trees. However, significantly better yields of fruit obtained in the year following the dormant season eradication of primary mildew, suggest long term beneficial effects of these treatments.     

Mechanism of resistance of Cladosporium cucumerinum against 6-azauracil and 6-azauridinemonophosphate

The mechanism of resistance of strain R of Cladosporium cucumerinum resistant to 6-azauracil (AzU), 6-azauridine (AzUR) and to 6-azauridinemonophosphate (AzUMP) has been studied. Conversion of AzU into AzUR proceeds in the wild strain N and the resistant strain N equally well. Both, strain N and strain R convert AzUR into AzUMP. However, the amount of AzUMP detected in the resistant strain was approximately, three times lower than in strain N. This may be due to a change in the enzyme uridine kinase. The poor ability to form AzUMP from AzUR resulted in a higher activity of the enzyme orotidine monophosphate decarboxylase (OMP-decarboxylase) in strain R in this way interfering less with the incorporation of orotic acid into RNA of strain R than in that of strain N.