El Desarrollo del Estómago del Vacuno: Análisis desde el Prisma Morfológico y Morfométrico Parte III: Reconstrucción Espacial y Morfometría

This work represents a summary of the study presented in parts I and II, previously published in this journal. The data collected in those studies is presented in three dimensional reconstructions of the stomach of the developing calf.     

El Desarrollo del Estomago del Vacuno: Análisis Desde el Prisma Morfológico y Morfométrico Parte II: Observaciones Morfogénicas Asociadas al Bloque Omaso-Abomásico

This study represents a continuation of part 1 of a study of the development of the bovine stomach. Material and methods are more fully described in part i of the study. In this part 2 of the study further developmental stages and morphometric analysis is presented.     

Estudio Histoquímico y Morfométrico de la Población Fibrilar del Músculo Diafragma. Parte I: Caballo y Perro

Histochemical and morphometric study of fibrilar population in diaphragm muscle. Part I: Horse and Dog By using m-ATPase techniques, under alkaline and acid pre-incubations, as well as metabolic techniques (α-MGPDH and NADH-TR), sections from the lumbar and costal portions of diaphragm muscle in horse and dog were analysed. Fibre samples were exposed to image-analysis observations in order to determine the lowest fibre diameter and obtain estadistical data. The findings revealed that the lumbar portion of horse diaphragm consists of fibre types I, IIA and IIB; in the costal portion, the fibre types I and IIA were present. In the dog, the lumbar and costal portions consisted of fibre types I, IIA and IIC, which showed some specific histochemical characteristics. Some data concerning both morphometrical and statistical fibre-type analysis are presented.     

Estudio Histoquímico y Morfométrico de la Población Fibrilar del Müsculo Diafragma. Parte II: Vaca y Cerdo

Histochemical and Morphometric Study of the Fibrilar Population in Diaphragm Muscle. Part II: Ox and Pig M-ATPase techniques, at different pH levels prior to incubation, as well as metabolic procedures (α-MGPDH and NADH-TR), were used to determine the fibre-type content of the lumbar and costal portions in the bovine and porcine diaphragm. The fibre samples were exposed to image analysis in order to evaluate both the morphometrical and statistical values. On the basis of tintorial reactions, the fibre types IA, IB and IIC were found in the lumbar portion of the bovine diaphragm; in the costal portion of the same diaphragm, IA, IB, IIA and IIC fibre types were observed. With regard to the porcine diaphragm, the I, IIA, IIB, IIC fibre types were present in both portions of the muscle. Further morphometrical and statistical data concerning all the fibre types are obtained and discussed.     

Anatomía del Desarrollo del Tracto Digestivo y Forma Corporal Externa Durante el Período Embrionario en el Caprino (Copra hircus)

Anatomy of the digestive tract and external body development during the embryonic stages of the caprine (Copra, bircus) The purpose of this work has been to establish the pattern of prenatal growth and normal development of the digestive tract and annex glands during goat embryonic stages. 21 embryos with ages ranging from 14 to 34 days (1.69 to 5.90 cm CR) as determined by registering the mating time, were obtained by cesarea. This material was histologically processed to obtain complete serial sections of the stomatodaeum, foregut, midgut, hindgut and cloaca. In this work, it is chronologically described the morphogenesis and histogenesis of the mouth, hypophysis, pharynx and its derivatives, stomach, intestine, liver, pancreas and cloaca. The results obtained establish chronological comparisons with the development of the lamb and gives information on the unitary origin of the gastric compartments in ruminants.     

Cronología de Osificación Postnatal de las Articulaciones del Hombro y Codo en el Gato Siamés (Felts catus L.)

Chronology of the postnatal ossification of the shoulder and cubit joints of the Siam cat (Felts catus L.) A study was done by radiological techniques to show the chronology of ossification of the shoulder and cubit joints in the Siam cat, from birth up to the 25th week of postnatal development. For this experiment we used 40 little cats (19 males and 21 females) belonging to 11 litters subjected to different controls: pattern race, healthy, feeding, growing up and radioactivity. The time of the appearance and the evolution of the ossification centers is determined as well as the phenomenon of fusion during this time. The postnatal ossification from both joints of the Siam cat is compared with that of the common cat.     

Estudio Histoquímico e Immunohistoquímico del Tejido Linfoide Porcino: Ganglio Linfático, Bazo y Timo

Immunohistological (S-100, cIg) and enzyme histochemical (ANAE/ANBE, beta-glu, ATPase, AcP) investigations were carried out to identify lymphocyte and reticular cell subpopulations "in situ", in pig lymphoid tissue (lymph node, spleen, and thymus) of a 6 months old group, and a 6-9 days old one. By means of immunohistological techniques, in the 6 month old pigs we could detect S-100 protein (PAP), chiefly in T-areas lymphocytes, but we also found some S-100 positive lymphocytes in spleen follicles. Also S-100 protein were detected at Follicular Dendritic Cells (FDC) in lymph node and spleen; and Reticular Fibroblastic Cells (RFC) only in the first one. Finally, S-100 were noted in Hassall corpuscles (thymus), nervous fibres, and endothelial cells too. Using PAP (IgG, IgM) and IPI (IgA) techniques we could detect lymphocyte cytoplasmatic surface immunoglobulins (cIg) in lymphocytes, lymphoblastoid and plasmacytoid cells in nearly all tissue compartments. By means of histochemical techniques we could identify T-area lymphocytes ANAE/ANBE and beta-glu positives (cytoplasmatic spots) and B-area lymphocytes ATPase positive; macrophages, and polymorphonuclear eosinophiles PHNE being ANAE/ANBE and beta-glu positives (diffuse cytoplasmic stain); and Hassall corpuscles ANAE/ANBE and AcP positives. Concerning to reticular cells, we found FDC and RFC in lymphoid follicles, and Interdigitating Reticular Cells (IDC) in lymphoid diffuse tissue, with enzyme activity (all the enzymes studied) in nearly all the cases. In piglets, the immunohistological and histochemical pattern was nearly the same.