Influence of long photoperiods on the growth of timothy (Phleum pratense L.) varieties from different latitudes in northern Europe

Plants of three varieties of Phleum pratense L. from different latitudes (cv. Engmo, 69°N; cv. S48, 52°N; cv. Motim, 52°N) were grown from the five-leaf stage at 12°C under continuous illumination or 8-h days at essentially identical daily inputs of radiant energy. The responses to daylength extension (increase in plant dry weight, plant height and leaf dimensions and reduction in the number of tillers per plant and in tissue dry matter content) were common to all three varieties and although the enhancement in plant dry weight and in leaf size was greater in the Engmo plants, this was principally the result of poorer growth and smaller leaf size in 8-h days. Marked differences between Engmo and the other varieties in the partitioning of dry matter within the plant were the consequence of differences in the rate of reproductive development. For example, compared with S48 and Motim, first spike emergence and 50% anthesis in the Engmo plants were delayed by 22 and 14 days, respectively, and 40% of the Engmo plants did not become reproductive even after 110 days of long-day treatment. Furthermore, by the time that 50% anthesis of the mainstem spike had been reached by the long-day plants of each variety, significant differences in tiller numbers and tiller fertility had developed between the varieties from high and lower latitudes. These findings and the results of previous studies of Poa pratensis, Dactylis glomerata and Bromus inermis are discussed in relation to the adaptation of high-latitude grasses to the Scandinavian environment.     

Influence of temperature on the digestibility of the tropical legume Macroptilium atropurpureum

The effect of temperature on the nutritive value of Macroptilium atropurpureum cv. Siratro and line 10–105 was determined in a controlled environment at day/night temperatures of 24/18, 30/24 and 36/30°C, and a 13-h daylength. Estimated dry matter digestibility, cell wall and lignin concentrations of leaf, stem and stubble were measured in two 5-week regrowth cuts.
Siratro and line 10–105 were similar in digestibility and responded similarly to temperature with whole tops, stem and stubble decreasing by an average of 0.0023, 0.0026 and 0.0056 units of digestibility for each 1°C rise in growth temperature. These changes reflect the increase in cell wall and lignin concentrations with higher temperature.
The digestibility of young, just-expanded leaves was increased by increased growth temperature. This effect may be explained by the faster rate of leaf expansion at high temperature which reduced cell wall and lignin concentrations at this stage of leaf development. However, for the bulk leaf fraction digestibility was unaffected by temperature. Leafiness declined at high temperatures.     

Effects of vernalization on the subsequent rates of leaf extension and photosynthesis of perennial ryegrass (Lolium perenne L.)

Temperature and daylength treatments have been used to investigate the apparent link between the potential for rapid leaf extension in spring and floral initiation and development in Lolium perenne (cv. Vigor). After growth at 5°C for 10 weeks (vernalization treatment) the leaves which developed at 15°C expanded significantly faster than non-vernalized controls under both long and short days. Under long days floral development was initiated in vernalized plants but under short days it was not. Specific leaf area was higher in vernalized than non-vernalized plants under long days.
The photosynthetic capacity of leaves which developed on plants which had been previously vernalized and then grown at 15°C under long days was higher than non-vernalized controls when expressed on a leaf area and dry weight basis, but not on the basis of chlorophyll content. Vernalization followed by short days resulted in a smaller and non-significant increase in the photosynthetic capacity of leaves.
Application of gibberellic acid (GA) as a spray to non-vernalized plants did not affect floral development but did increase the rate of leaf extension. The application of GA did not affect specific leaf area or photosynthetic capacity expressed on an area basis.     

Competition among seedlings of phalaris, subterranean clover and white clover in diallel replacement series mixtures

Phalaris, subterranean clover and white clover were grown together in binary, diallel replacement series mixtures at (day/night) 15/10 or 24/19°C under long-day conditions, and cut at 2- or 4-weekly intervals. Analysis of white clover mixtures was confined to 15/10°C due to losses of seedlings prior to imposition of treatments at 24/19°C.
With white clover at 15/10°X, seedling losses occurred after the first harvest, the greater losses occurring in mixtures where subterranean clover was the major component. The de Wit analyses showed that subterranean clover excluded its companions, whereas phalaris and white clover competed for slightly different niches resulting in over-yielding. Gleeson McGilchrist analyses showed that subterranean clover and phalaris were more aggressive than white clover under infrequent cutting, but that white clover was more aggressive as a major than as a minor component of a mixture under frequent cutting. Between phalaris and subterranean clover [he pattern of competition at 24/19°C differed from that at 15/10°C only in the more rapid development of subterranean clover and consequent swing to phalaris dominance.
We conclude that the seedlings of white clover and subterranean clover are suited to combination with phalaris because phalaris is not too aggressive towards white clover, and is tolerant of the aggressiveness of subterranean clover. The survival of only a few seedlings of white clover in mixtures with subterranean clover may be sufficient for long-term growth provided some perennation occurs after the senescence of subterranean clover.     

EFFECT OF DENSITY OF PLANT POPULATION, PHOTOPERIOD, TEMPERATURE AND LIGHT INTENSITY ON INFLORESCENCE FORMATION IN WHITE CLOVER

During 1959–62 a study was nmdc of the effect or the density of plant population, photoperiod, temperature and light intensity on stolon rornialion, floral induetion, bud expansion and inflorescence development in S184 white clover.
The density of the plant population alfected not only stolon and inflorescence formation, but also the root system. The'open' sward with about 60 plants per sq. yd gave the highest number of inflorescences per unit area, and al the same time contained few plants with tap roots.
White clover had a long-day photoperiodjc requirement, but there was variation between the individual plants in their requirements of daylength, temperature and light intensity.
Overwintering did not appear to be essential for floral induction and primordia development. Too low or too high temperatures during the overwintering period appeared, respectively, to delay or stimulate vegetative growth, while both extremes of temperature seemed to delay floral induction and reduce inflorescence formation. A medium range of temperature (40°–55°F) followed by an increasing temperature, daylength and light intensity, appeared to be most satisfactory for primordia initiation and flowering.
There is some inter-relation between temperature and light intensity, but light intensity stimulates vegetative growth and development of the primordia, as well as being essential for the formation of numerous inflorescences.     

Growth of Trifolium repens L. and Trifolium semipilosum Fres. Var. glabrescens Gillet at different temperatures in controlled environments and in the field

The growth of Trifolium semipilosum Fres. var. glabrescens Gillet cultivar Safari, and Trifolium repens L., cultivars Haifa and Grasslands Huia was compared in controlled temperature glasshouses at 15/10, 21/16, 27/22 and 33/28°C (day/ night), and over eighteen months under four-weekly cutting in the field. In glasshouses, white clovers grew better than Safari at all temperatures except 27/22°C. Stolon production and partitioning of dry matter to stolons was greater, and petiole production and partitioning of dry matter to petioles was less for Safari than for white clovers. Haifa and Huia differed primarily in individual leaf weight, individual leaf area and leaf numbers; with Hula and Safari, but not Haifa, exhibiting a reduction in leaf size in response lo defoliation. In the field, growth of Safari was poor. Growth responses of Haifa and Huia in the field were related to seedling vigour, dry matter partitioning between leaves and stolons, and response to defoliation at low temperatures. It was concluded that there were major differences in growth, dry matter partitioning, morphology and response to temperature between T. semipilosum and T. repens. These differences particularly influence climate adaptation and competitive ability at the seedling stage.     

Growth of prairie grass (Bromus willdenowii) and tall fescue × perennial ryegrass (Festuca arundinacea×Lolium perenne) on the Appalachian Plateau of southern West Virginia, USA

The productivity and persistence of prairie grass ( Bromus willdenowii Kunth) cv. Grasslands Matua were compared with a similarly managed endophyte-free tall fescue × perennial ryegrass hybrid ( Festuca arundinacea Schreb. × Lolium perenne L.) cv. Johnstone. A field study was conducted on the Appalachian Plateau of southern West Virginia, USA (81°W, longitude; 38°N, latitude; 850m above sea level) for three consecutive years. Nitrogen totalling 0, 168, and 336 kg N ha⁻¹ year⁻¹ was applied to stands managed as hay. Annual herbage yields were similar for both species in year 1 (1989), but by year 3 (1991) Matua sown-grass yield was about 35% of first-year yields. Total herbage yield for Matua plots in the third year was similar to first-year yields owing to encroachment by non-seeded grasses and white clover ( Trifolium repens L.). Matua was susceptible to powdery mildew [ Blumeria graminis (DC) E. O. Speer] in this environment, and was similar in productivity (first year only) and nutritive quality to tall fescue × perennial ryegrass hybrid. Matua use may be limited to areas where low-temperature stress and resultant winter damage is unlikely to occur.     

Effect of temperature regime on germination of seed of perennial ryegrass (Lolium perenne)

Perennial ryegrass ( Lolium perenne L.) is one of the most valuable temperate pasture grasses in the world. Understanding seed germination in response to temperature can help determine its optimal seeding times. Germination tests for two cultivars of perennial ryegrass (cv. 'Turfstar' and cv. 'Transtar') were conducted by applying thirty-six constant and alternating temperature regimes, ranging from 5 to 40°C (16 h night/8 h day). Responses in germination rate to these temperature regimes were then used to construct a quadratic response surface, giving estimated germination rates with confidence intervals at P  ≤   0·05 for both cultivars. Optimum germination rate occurred over a range of regimes: 15/25, 20/25, 20/30 and 25/30°C. Furthermore, a wide range in diurnal alternating temperatures resulted in maximum seed germination rate for cv. 'Transtar', while no constant temperature regime supported maximum germination rate for cv. 'Turfstar'. A temperature-dependent germination rate function for perennial ryegrass was developed which could be used in the future to predict location-specific optimum seeding times when national or regional monthly temperature data are available.     

Solanum microdontum (PI 558098): A diagnostic host plant for potato virus A

Solanum microdontum (PI 558098) develops diagnostic symptoms when inoculated with plant sap containing potato virus A (PVA). The symptoms consist of local lesions (2–4 mm), followed by systemic necrosis of leaf veins, bronzing of leaf surface, and leaf drop. Symptoms develop in a temperature range of 20–29 C with 4 to 8 Klux light intensity of 14 h daylength. Local lesions are visible within 1 wk of inoculation and can be caused by PVA containing sap dilutions of up to 1:100. Plants multiplied by shoot cuttings as well as those produced from true potato seed are equally sensitive. True potato seed production occurs under normal greenhouse conditions in cross-pollinated plants.     

Petiole analysis and the nitrogen fertilization of Russet Burbank potatoes

The effect of nitrogen rates on the yield of Russet Burbank potatoes was studied in field experiments in Idaho. Petioles were sampled at the 6-to-8 leaf stage and thereafter at two week intervals until mid August. Petiole nitrate concentrations were very high early but decreased rapidly as the season progressed and declined to a very low level as the plants matured. The nitrate content of the petioles reflected the amount of N applied to both locations. Nitrogen applications increased total yield and the quantity of the larger size tubers. A highly significant correlation was found between the early season petiole nitrate concentrations and total yield. Suggested ranges of petiole nitrate concentrations were developed as a guide to efficient N fertilization of Russet Burbank potatoes in Idaho.     

The relationships between stolon characteristics, winter survival and annual yields in white clover (Trifolium repens L.)

Five white clover populations of Swiss origin and three bred varieties were grown in binary mixtures with two perennial ryegrass varieties, Aurora and S23. The seasonal yields of clover and grass plus clover were measured under a cutting regime during the second and third years after establishment. A series of destructive detailed sward measurements was made during the late autumn to spring period preceding each harvest year. In this way changes in the amounts of stolon, leaf plus petiole and numbers of growing points were monitored during the winter.
There were large differences in clover yield between populations in both years. These were evident from the first (spring) harvest in each year. Higher-yielding clovers in spring tended to produce higher annual clover yields. No grass × clover interaction was evident at any harvest. Large differences between clovers were also apparent in the morphological characteristics measured, with the Swiss material generally having greater amounts of stolon, leaf plus petiole and numbers of growing points present in early spring. It is proposed that these factors contribute to the high spring yield in the Swiss populations. Loss of stolon length over each winter was less in the Swiss material, indicating that its good spring growth was not obtained at the expense of winter hardiness. Annual clover yield was found to be significantly positively correlated with the amount of stolon present in spring, exemplifying the importance of stolon survival over the winter.     

EFFECT OF DAY-NIGHT TEMPERATURE REGIMES ON GROWTH AND MORPHOLOGICAL DEVELOPMENT OF TIMOTHY PLANTS DERIVED FROM WINTER AND SUMMER TILLERS

Winter (vernalized) and summer (non-vernalized) timothy tillers were grown to anthesis in day/night temperature regimes of 32/26°, 27/21°, 21/15°, and 15/10°C. Herbage and total plant yields, total leaf-blade number and area, and total plant growth-rate were highest, or very nearly so, in the 21/15°C regime for both the winter and summer plants. However, summer plants reached anthesis 13 to 14 days later than winter plants in each temperature regime. Even though virtually all primary shoots produced an infiorescence, summer plants had significantiy more leaves and leaf-blade area, produced significantiy higher yields of leaf blade, stem plus sheath, stubble, and root tissues and had a significantly higher total plant growth rate than winter plants in each temperature regime.     

The effects of nitrogen fertilizer applied in spring on swards of ryegrass sown with four cultivars of white clover

Nitrogen fertilizer was applied at 0, 30,60 and 90 kg N ha^-1 in March 1976, 1977 and 1978 to plots containing perennial ryegrass cv. Barlenna sown either alone or with white clover cv. Blanca, Sabeda, Olwen and S100 in four replicated blocks.
Dry matter (DM) yield of all swards responded positively to N with the response being highest in the no-clover swards in two of the three years. Annual DM yields were lower in the no-clover than in the mixed swards at any given N level. The higher levels of N reduced DM yield in mixed swards at some harvests in midsummer.
Swards of S100 were consistently among the lower yielding mixed swards, whereas the other three clover cultivars varied in their relative yields. There were no N x cultivar interactions for white clover yields; irrespective of cultivar, N application reduced annual white clover yield by similar amounts.
It is concluded that it is beneficial to apply moderate amounts of N fertilizer to mixed swards in spring and that under these conditions the yield differences between swards including medium-large and smaller leaved white clover cultivars are similar to differences between the inherent yield potential of these cultivars in mixed swards receiving no N.     

Leaf number as a criterion for determining defoliation time for Lolium perenne: 2. Effect of defoliation frequency and height

The object of this study was to determine the importance of frequency and height of defoliation on regrowth potential of Lolium perenne. Defoliation interval was based on stage of the regrowth cycle, as indicated by leaves per tiller.
Simulated swards of Lolium perenne cv Yatsyn were grown as individual plants in a glasshouse kept at a day/night temperature of 25°C/15°C.
Treatments imposed were defoliation at 2, 5 or 12 cm residual height, and low and high water soluble carbohydrate (WSC) level obtained by varying defoliation interval, i.e. defoliating at the 1-leaf or 3-leaf stage of the regrowth cycle. Regrowth after frequent short defoliations was only 65% of the less frequently defoliated plants taken over the full regrowth cycle. This was associated with a lower stubble WSC content (2·15 vs 17·5% in stubble) and a twenty-seven-fold difference in the amount of WSC in the stubble per plant. This difference in total WSC was a combined effect of more and heavier tillers and higher WSC content in stubble of plants defoliated less frequently at the end of the regrowth cycle. The regrowth of plants with WSC levels depleted by frequent defoliation when defoliated at 2 cm was significantly below that of those defoliated at 5 and 12 cm.
The results indicate the desirability of defoliating plants at the 3-leaf stage of the regrowth cycle. This not only allows the full regrowth potential to be expressed in that growth cycle, but also in the next cycle, by allowing the replenishment of WSC reserves and optimizing tiller status. The potential to regrow appears then to be based more on the total amount of WSC than the proportion of WSC in stubble.     

Competition between white clover (Trifolium repens L.) and subterranean clover (Trifolium subterraneum L.) in binary mixtures in the field

White clover, (cv. Haifa) and subterranean clover (cv. Seaton Park) were grown in binary replacement series mixtures in the field at proportions of 100:0, 90:10, 50:50. 10:90 and 0:100, and cut every four weeks over three growing seasons from 1985 to 1987 at Scone, New South Wales (NSW) (32°S).
Relatively low plant densities minimized competition in 1985, but in subsequent seasons Haifa and Seaton Park competed vigorously for the same resource;.; usually having relative crowding coefficients greater than 1.0, and relative yield totals greater than 1.0 in spring of 1986 and 1987, and autumn 1987. This over-yielding appeared to be due to some complementarity of leaf morphology and canopy architecture.
Haifa severely depressed seed production of Seaton Park in mixtures. Haifa regenerated as an annual in 1986, but as a perennial, from surviving stolons, in 1987 owing to lower maximum temperatures and better rainfall distribution in late summer and autumn of 1987.
It is concluded that white clover can survive severe competition from subterranean clover as a seedling, and if moisture allows, may severely depress seed production from subterranean clover, regardless of whether plants are annual or perennial in origin. Spatial separation may minimize competition in average years but competition effects may be severe in seasons with sufficient summer rainfall for perennation of white clover, and seasonal autumn rainfall for germination of subterranean clover.     

Growth and Nutrient Accumulation of Winged Bean and Velvet Bean as Cover Crops in a Subtropical Region

We examined biomass dry matter and nutrient uptake of live plant parts, leaf area index, and litter of winged bean (Psophocarpus tetragonolobus) and velvet bean (Mucuna pruriens) 12, 18, 24 and 30 weeks after sowing (WAS). The two plants had similar leaf and stem+petiole biomasses. At 30 WAS winged bean had a significantly lower pod yield than velvet bean. Between 18 and 30 WAS, winged bean produced less litter than velvet bean due to differences in growth stages. The total mulch of live parts and litter of winged bean and velvet bean completely covered the ground by 18 and 12 WAS, respectively. Compared to velvet bean, the leaf and stem+petiole of winged bean had a significantly higher N concentration; significantly higher N uptake at 24 and 30 WAS; significantly lower C/N ratio; and significantly higher P, K and Mg concentrations. In winged bean, P uptake was significantly higher in the leaf at 30 WAS and in the stem+petiole at all harvesting times. The total biomass of the leaf, stem+petiole and litter of winged bean was 317–561 g DM m-², and their N content was 12.3–17.7 g m-². The total biomass of live parts and litter of winged bean might be sufficient to suppress weeds and increase soil N. Winged bean is an appropriate legume cover crop and green manure due to its longer growing period and superior ground-covering ability and high N input.     

Changes in contribution of white clover to canopy structure in perennial ryegrass/white clover swards in response to N fertilizer

Twelve plots were laid down on an existing perennial ryegrass/white clover sward, one plot in each of six replicated blocks receiving 100 kg  N ha⁻¹ (100N) and one plot receiving no N (0N). Biomass, canopy development (stratified cuts and point quadrat records at 2–4-week intervals) and changes in stolon population density were recorded during one 8-week regrowth period (25 July–23 September) to investigate the likely causes of N effects on white clover in mixed swards.
Over the period, N fertilizer resulted in an increase of 74% in perennial ryegrass biomass and a reduction of 24% in white clover biomass. There was also a reduction of 44% in stolon growing point density, mainly due to lower density of younger stolon branches. White clover's contribution to the upper three leaf area index (LAI) units (taken as an estimate of the proportion of photosynthetically active radiation (PAR) intercepted) was, on average, 70% at 0N producing 74% of the sward biomass, compared with 46% contribution to interception and 37% contribution to biomass at 100N.
While there was no evidence of overtopping, it is concluded that N fertilizer application increased the LAI of perennial ryegrass in the upper layers of the canopy thereby reducing the share of available PAR to white clover. This, coupled with a lower radiation use efficiency at high N and lower population density, results in white clover's reduced performance in mixed swards receiving N fertilizer.     

Internodal elongation: A potential screening technique for heat tolerance in potato

Summary In pot culture experiments over 2 consecutive years, 20 potato genotypes of varying heat tolerance were grown under long day conditions and heat stressed by being exposed to high (38 °C/21 °C mean day/night) temperature. The percent increase in mean internode length of heat stressed plants over those grown at normal temperatue (25°C/16°C day/night) was correlated with the percent tuber dry matter yield of the heat stressed plants (r=0.618, P=0.01). In the same 20 genotypes, grown under short day conditions at normal temperature, the fraction of leaf bud cuttings exposed to high night temperature (23°C) that produced tuber initials correlated with the percentage increase in internode elongation in stem cuttings exposed to 25°C compared with those exposed to 15°C (r=0.680. P=0.01). Thus the relative changes in internode elongation are related to thermal tolerance, and it is suggested that this can be used as a selection criterion for heat tolerance.     

Evaluation of white clover varieties under grazing and their role in farm systems

Four experiments were established in 1981–84 to investigate the effect of defoliation treatments on white clover varieties when grown with S23 perennial ryegrass. Treatments included a cutting only regime, as used in National List trials, and grazing systems simulating as near as possible those used on farms.
Differential effects of cutting and continuous sheep stocking on white clover varieties, together with significant variety × defoliation interactions, illustrated the importance of the grazing animal in the evaluation of white clover. Differential effects of cattle and sheep grazing were also evident. Cattle grazing was less detrimental to white clover than was sheep grazing, i.e. cattle were not selective, and less stolon was removed. In general, with both cattle and sheep grazing the larger the clover leaf size the greater the loss in stolons, which in turn decreased persistency. The results illustrate how alternating cutting, sheep grazing and cattle grazing managements can be used to maintain optimum clover/grass balance.
Successful clover/grass swards depend on the retention of clover, yet avoiding clover dominance. The difference in N transfer between clover varieties, especially those within the same leaf category, and the extra grass produced without fertilizer N, emphasized the importance of varietal choice. Breeding programmes have been concerned with the selection of larger-leaved, long-petioled varieties for growing in competition with grass in the presence of fertilizer N. However, the present results showed that, under continuous sheep stocking, increase in leaf size does not increase clover yield or persistency.
The results presented emphasize the importance of the grazing animal in the evaluation of white clover varieties and indicate that yield of clover dry matter should not be the major criterion for selection of varieties for farm systems.     

The effects of variety, cutting interval and nitrogen application on the morphology and development of stolons and leaves of white clover

Six varieties of white clover, each grown with perennial ryegrass, four intervals between cuts and two levels of applied nitrogen in all combinations, were compared in a field experiment during the first 27 months after sowing. Information about yields, crop fractions, heights and ryegrass tillers has been presented in an earlier paper (Wilman and Asiegbu, 1982). The present paper is concerned with the more detailed studies of white clover, which help to explain the yield results and contribute to the understanding of the response of this species to management when grown in competition with grass. Increasing the interval between harvests increased the length of clover stolon per unit area of ground and increased stolon diameter, petiole length, weight per leaf and number of leaves harvested as a proportion of the number present in the sward while only slightly reducing the rate of leaf emergence, helping to explain the positive effect of increasing the interval on clover yield noted in the earlier paper. During regrowth, successive leaves had longer petioles and the length of individual petioles increased beyond the stage at which the leaflets were fully opened. Weight per leaf in clover increased considerably from April to June and declined to below the April value by October. It was shown that weight per leaf can be greatly increased by increasing the interval between harvests without reducing the number of leaves harvested per unit area per year. The stolon length measurements provided some support for the view that medium large-leaved varieties of white clover can with advantage be defoliated rather less frequently than small-leaved varieties. Stolon length was less adversely affected by applied N in the medium large- than in the small-leaved varieties. The small-leaved varieties had thinner stolons than the medium large-leaved varieties but about twice the stolon length when no N was applied, and a relatively high proportion of leaves which escaped defoliation. The application of N reduced stolon diameter, increased petiole length and had little or no effect on weight per clover leaf.