Hemopoietic colony-forming units in regenerating mouse liver: suppression by anticoagulants

After hepatic injury induced by carbon tetrachloride, mitotically active hematopoietic cells of nonhepatic origin localize in the liver as judged by an increase in colony-forming nodules in the spleens of lethally irradiated recipient mice on intravenous injection of cells from these livers. The administration of warfarin suppresses the localization of colony-forming units in the regenerating liver by inhibiting the coagulation mechanism of the donor animals.     

鸡贫血因子病的免疫病理学变化

 用鸡贫血病毒（CAV）感染1日龄健康AA雏鸡，以未感染同龄雏鸡为对照，在感染后7、14、21、28、35、42和49d检测其免疫器官与体重比值，胸腺、脾脏T细胞白细胞介素2（IL-2）和干扰素（IFN）诱生活性，胸腺、法氏囊、脾脏T、B细胞增殖反应，胸腺、法氏囊、脾脏、盲肠扁桃体及哈德尔腺中α-萘酚酯酶阳性[ANAE#+（+）]T细胞、IgG、IgM、IgA抗体生成细胞数量，外周血液中T、B细胞数量，血清、泪液、气管液、肠液、胆汁中IgG、IgM、IgA含量的动态变化。揭示了感染鸡细胞因子IL-2、IFN的免疫调节发生障碍、中枢与外周免疫器官的细胞免疫和体液免疫功能呈现抑制，呼吸道与消化道的局部免疫防御功能降低。     

Lunar receiving laboratory

The Lunar Receiving Laboratory will be the permanent depository of a portion of the collection of lunar samples; it will safeguard the collection, providing continuing security and ensuring scientific integrity. In carrying out the time-dependent experiments and continuing functions of the laboratory, NASA will rely on visiting expert scientists supplementing a relatively small resident staff; outside scientists will be relied upon for most investigations and detailed analyses of samples. It is believed that the designed procedures and facilities provided will ensure the maximum scientific return from the Apollo Program in the way of information from lunar samples.     

奶牛真胃移位继发贫血的治疗

对23例贫血的真胃移位奶牛进行手术治疗并用新生犊牛全血输血1次后,经过7 d左右的住院治疗和3个月的临床观察,病牛痊愈,乳产量几乎恢复正常.     

犬免疫介导溶血性贫血的诊治

免疫介导溶血性贫血是一种与自身红细胞抗体有关的溶血性贫血。患犬高度贫血和溶血,死亡率高达40%以上,多为再生性贫血。可采取支持疗法,缓解贫血、脱水和继发并发症。同时结合药物治疗抑制免疫系统,使红细胞免受攻击并促使骨髓产生新的红细胞。     

鸡传染性贫血病毒分子生物学研究进展

对国内外鸡传染性贫血病毒基因组及其编码蛋白等方面的最新研究成果进行了论述。     

Cigarette smoke contains anticoagulants against fibrin aggregation and factor XIIIa in plasma

Gas-phase, water-soluble components of cigarette smoke cause delayed fibrin self-assembly and prevent fibrin cross-linking by inactivation of factor XIIIa (plasma transglutaminase). These anticoagulant properties of smoke are demonstrable in plasma, suggesting they play a role in the pathophysiology of smoking.     

Noncytokinetic radiation injury: anticoagulants as radioprotective agents in experimental radiation hepatitis

Rats that have been irradiated with a single dose of 1500 roentgens directed to the liver show the following abnormalities: increased clearance of colloidal gold by the liver at 30 days, a decreased albumin space index at 30 days, and increased alkaline phosphatase at 60 days. In each of these cases, animals receiving Depo-heparin in dose ranges reasonable for human application had essentially normal values. There was no radioprotective effect with salicylates at the doses used. Higher doses of salicylates resulted in death of the animals.     

4种抗凝剂对刺参体腔细胞的抗凝效果

研究了不同浓度的柠檬酸钠、肝素钠、乙二胺四乙酸二钠(EDTA-Na2)和草酸钠对刺参Stichopus japonicus体腔细胞的抗凝效果。结果表明,3.8 mg/mL的柠檬酸钠、1.0~1.5 mg/mL的ED-TA-Na2、0.2~0.6 mg/mL的肝素钠、1.0~1.5 mg/mL的草酸钠均能较好地保持体腔细胞的形态活性,适合用作刺参体腔细胞的抗凝剂。4种抗凝剂中EDTA-Na2的效果最好。     

犬贫血性疾病输血疗法的疗效观察

输血疗法是救治动物的一项重要措施。为了验证输血疗法的作用,对数十例贫血病病犬进行输血疗法治疗,通过血常规检查、血液生化学检查等发现患犬均患有不同程度的贫血,红细胞数、血红蛋白含量、红细胞压积均明显低于正常值,在治疗原发病的同时配合了输血疗法。经过一段时间的治疗,发现应用输血疗法治疗的患犬痊愈快,患犬的红细胞数、血红蛋白含量、红细胞压积均逐渐升高接近正常值。由此可见,输血疗法对犬贫血病的治疗效果较好。     

硒缺乏致发雏鸡贫血机理的研究

为探讨雏鸡硒缺乏时发生贫血的机理,选用1日龄健康海兰褐雏鸡120只,随机分成2组,每组60只,第1组为试验组,饲喂低硒饲料(硒含量为0.014 mg.kg-1);第2组为对照组,饲喂全价饲料(硒含量为0.375 mg.kg-1).自由采食与饮水.分别于雏鸡10,20,30,40 d时每组选10只鸡心脏采血1 mL,离心分离血清,应用放免法测定血清中的细胞因子和甲状腺激素的含量.结果表明,硒缺乏组雏鸡血清中T3,FT3,IL-1和EPO含量较正常对照组有较大幅度降低,差异极显著(P<0.01);TNF,T4和FT4含量较正常对照组有较大幅度升高,差异极显著(P<0.01).硒缺乏雏鸡发生了3种类型的贫血.     

改良溶血性贫血动物模型的建立

[目的]建立一种改良的小鼠急性溶血性贫血模型,为临床药物试验提供依据。[方法]采用连续用药方式对昆明小鼠皮下注射盐酸苯肼来制备动物模型,每日检测网织红细胞的比例、红细胞数量、白细胞数量、血红蛋白含量。[结果]血液检测显示,用药2～3 d后小鼠开始出现贫血现象,第7天左右红细胞数量降低60.0%以上,网织红细胞比值最高达19.5%,同时血红蛋白含量降低约67.0%。[结论]改良的小鼠急性溶血性贫血模型制备方法可行,符合血液学检测标准。     

鸡贫血病毒衣壳蛋白基因的原核表达

[目的]为VP1蛋白的免疫学研究和鸡贫血病毒基因工程疫苗的研制奠定基础。[方法]将1个新克隆的鸡贫血病毒vpl基因（AF448446）在大肠杆菌DE3中进行表达,并对该蛋白进行纯化。[结果]结果表明：已成功构建了表达载体pET30（b^＋）-vp1;VP1蛋白已成功诱导表达并得以纯化;获得的VP1蛋白序列与已发表的VPl蛋白序列存在差异。[结论]试验克隆的vp1基因大小为1347bp,编码449个氨基酸的蛋白。     

消化道肿瘤贫血患者促红细胞生成素与贫血治疗方案的关系

目的分析消化道肿瘤患者内源性促红细胞生成素水平及贫血状态,探讨使用重组人促红细胞生成素治疗消化道肿瘤相关性贫血的方案.方法内源性促红细胞生成素采用Elisa检测,血红蛋白采用常规检测.消化道肿瘤非贫血患者为对照组,消化道肿瘤贫血患者组为观察组.结果统计发现男女之间的贫血率没有差异,消化道肿瘤相关性贫血患者占38.95%,其中以胃癌居首,占42.2%,食管鳞癌最低,占35.6%.消化道肿瘤患者在化疗前贫血比非贫血患者EPO高而Hb低,两者存在逆相关关系,化疗后贫血患者Hb继续降低,EPO上升;而在化疗后非贫血患者中出现的26例贫血患者,其中16例出现EPO急剧上升,10例EPO几乎不变,EPO存在极显著差异,具有统计学意义,而Hb没有明显的差异.结论消化道肿瘤相关性贫血发生率较高,在临床治疗中要密切关注.