共查询到20条相似文献,搜索用时 31 毫秒
1.
Yuuri Hirooka Seiju Ishikawa Jun Takeuchi Hiromichi Horie Kiichi Nakayama Motoo Koitabashi Seiichi Okuda Keiko T. Natsuaki 《Journal of General Plant Pathology》2009,75(1):83-86
Based on inoculation experiments and morphological and molecular phylogenetic studies, Cylindrocladium damping-off of strawberry
and Cylindrocladium blight of coral bells in Japan were demonstrated to be caused by Cylindrocladium canadense. This is the first report of a disease on strawberry and coral bells caused by this fungus. 相似文献
2.
Naho Yamagishi Junji Nishikawa Youichi Oshima Naoki Eguchi 《Journal of General Plant Pathology》2009,75(5):401-403
A black spot disease on cultivated alstroemeria was found in Nagano Prefecture, Japan, in January 2008. The causal fungus
isolated from the diseased plants was morphologically identified as Alternaria alstroemeriae E.G. Simmons & C.F. Hill. An inoculation test with the isolated fungus demonstrated that the disease is caused by this species.
This is the first report of black spot on alstroemeria (kokuhan-byo, in Japanese) caused by A. alstroemeriae in Japan. 相似文献
3.
Sílvia Tavares João Inácio álvaro Fonseca Cristina Oliveira 《European journal of plant pathology / European Foundation for Plant Pathology》2004,110(10):973-982
The ascomycetous fungus Taphrina deformans is the agent of peach leaf curl, a worldwide disease of peach potentially devastating to both crop yields and tree longevity. Conspicuous leaf curl symptoms result from the invasion of host tissue by the strictly parasitic mycelial phase of the T. deformans dimorphic life-cycle. Successful isolation of the fungus in pure culture is cumbersome and limited to late spring/early summer (time of ascospore discharge from infected leaves) and only rarely has the asymptomatic yeast phase been isolated from buds. Molecular methods, namely those based on the hybridisation of nucleic acids, are advantageous for diagnostic purposes since they do not require isolation of the fungus on culture media. Direct amplification using the polymerase chain reaction (PCR) and fluorescent in situ hybridisation (FISH) were tested for diagnosis of peach leaf curl disease in order to provide a fast and reliable method for disease risk assessment. Specific primers and probes were designed based on available ribosomal DNA sequence data. Positive and specific diagnoses of peach leaf curl were achieved with primer TDITS1, using PCR-detection, and probe TDE634, using FISH, both on infected leaves and in washings of asymptomatic peach buds. 相似文献
4.
Keisuke Tomioka Yuuri Hirooka Toshirou Nagai Hiroyuki Sawada Takayuki Aoki Toyozo Sato 《Journal of General Plant Pathology》2011,77(4):266-268
Severe blight of potted seedlings of monkshood caused by Plectosporium tabacinum was found in glasshouses in Kagawa Prefecture in southwest Japan in May 2001. Root rot and browning of stem bases were followed
by wilting and yellowing of leaves, then blighting of leaves, flower buds and stems. A fungus was isolated from diseased plants
and confirmed to cause the disease. The new disease was named “Plectosporium blight of monkshood”. 相似文献
5.
Black Spot of Peach Caused by <Emphasis Type="Italic">Alternaria alternata</Emphasis> (Fr.) Keissler
A new disease of peach (Prunus persica Batsch var. vulgaris Maxim.), causing brown, sunken lesions and brownish to blackish brown spots with cracks on peach fruits, was found in Okayama
prefecture, Japan, in 1995. The disease was observed not only on peach fruits but also on twigs and leaves. An Alternaria sp. was consistently isolated from these diseased fruits, twigs and leaves. The isolates were pathogenic to peach fruits
and leaves. Based on the morphological characteristics, the causal fungus was identified as Alternaria alternata (Fr.) Keissler. After cross-inoculation with isolates from peach, Japanese pear and apple, the isolates were found to be
pathogenic only to their original host. This is the first report on a peach disease caused by a host-specific A. alternata; therefore, the common name of black spot (`Kokuhanbyo' in Japanese) was proposed.
Received 25 June 1999/ Accepted in revised form 12 October 1999 相似文献
6.
Franck Rivano Malena Martinez Victor Cevallos Christian Cilas 《European journal of plant pathology / European Foundation for Plant Pathology》2010,126(4):541-552
Resistance of rubber tree clones to South American Leaf Blight (SALB) caused by the fungus Microcyclus ulei is normally assessed in specific large-scale clone trials, which in general entail a considerable amount of work. Four variables
are observed monthly on each tree over many years: disease severity and conidial sporulation intensity on young leaves, and
severity and stroma density on mature leaves. In order to simplify this field assessment method, we tested the resistance
of eight rubber tree clones to M. ulei in Ecuador in a Fisher block design with four replicates per treatment. Three months after planting, monthly observations
were made for a period of 12 months on the foliage focusing on the four variables, in order to quantify disease development.
Given the correlations between the four variables, assessment of conidial sporulation intensity on young leaves and stroma
density on mature leaves should be sufficient. The most suitable period to start the assessment was 6 months after planting,
for a duration of six to nine months. As repeated observations on the same trees were autocorrelated, it was possible to reduce
the assessment frequency to once every 2 months. To conclude, assessing the resistance of rubber tree clones to SALB in large-scale
clone trials can be optimized to reduce the number of observation times by 50%. 相似文献
7.
洛阳自古便是著名的牡丹之乡,其牡丹栽培始于隋,鼎盛于唐,宋时甲于天下。据统计,洛阳共有牡丹品种1 000多个,占全国的67.25 %,已成为中国乃至世界牡丹的栽培中心之一 相似文献
8.
Yuuri Hirooka Takao Kobayashi Jun Takeuchi Tsuyoshi Ono Yasunori Ono Keiko T. Natsuaki 《Journal of General Plant Pathology》2007,73(5):330-335
A ring spot disease of Aloe vera was found on leaves of potted seedlings of Aloe vera in Hachijojima and Chichijima Islands, Tokyo. From tissue of ring spot lesions, a fungus producing Fusarium-type conidia was consistently isolated. After 1 month, reddish perithecia of nectriaceous fungus had formed on the colonies
of this isolate on PDA. These nectriaceous and Fusarium fungi were identified as Haematonectria haematococca and Fusarium sp., respectively. From a single ascospore isolation, the former was confirmed to be the teleomorph of the Fusarium sp. Typical ring spot lesions were reproduced by artificial inoculations using single ascospore and single conidium isolates.
Inoculations of five species of genus Aloe revealed that they were highly susceptible except for A. arborescens. This is the first report of a disease on Aloe caused by H. haematococca (anamorph: Fusarium sp.) in Japan, and it was named aloe ring spot. 相似文献
9.
In June 1995, a disease causing round to irregular-shaped, water-soaked, brown to blackish brown spots on mealycup sage (Salvia farinacea Benth.) was found in Atsugi-shi, Kanagawa Prefecture, Japan. The symptoms were seen only on leaves, not on neither flower
petals or stems. The disease was also found in Setagaya-ku, Tokyo, Memambetsu-cho, Hokkaido and Shimoda-shi and Matsuzaki-cho,
Shizuoka. An Alternaria sp. was frequently isolated from these diseased plants. The isolates were severely pathogenic to mealycup sage and caused
lesions on the inoculated leaves. The isolates were also weakly pathogenic on scarlet sage (S. splendens Sellow ex Roem. and Schult.) but not on any other Labiatae plants tested. Based on morphological characteristics, such as
size of conidia, chain number, and the short beak on conidia, the causal fungus was identified as Alternaria alternata (Fr.) Keissler. This report is the first on a mealycup sage disease caused by A. alternata. Because the symptom was restricted to the leaf, the common name of Alternaria leaf spot was proposed.
Received 30 August 2002/ Accepted in revised form 18 November 2002 相似文献
10.
A smut-like disease was found on the leaves of Sagittaria latifolia in Japan. Spore balls collected from the leaves of S. latifolia and S. trifolia var. edulis were used to cross-inoculate leaves of pathogen-free plants of the two species to identify the pathogen. Spots and swellings
formed on leaves of the two species 10 days after inoculation. These symptoms were quite similar to those of the leaf smut
disease of S. trifolia var. edulis caused by Doassansia horiana, and the spore balls were characteristic of the fungus. Therefore, the authors conclude that D. horiana caused leaf smut disease on S. latifolia.
Received 18 January 2000/ Accepted in revised form 14 May 2000 相似文献
11.
为明确在福建省南平市的橘柚和三明市的温州蜜橘上发现的疑似柑橘褐斑病的病原菌种类,采用组织分离法获得纯化菌株,通过回接法验证菌株的致病性,利用形态学特征对病原菌进行初步鉴定,并采用最大似然法以多聚半乳糖醛酸酶基因endoPG为靶标对本研究以及国内外已报道的链格孢菌株构建系统发育树,分析其遗传多样性。结果表明,从病组织中共分离获得26株纯培养菌株,经形态学鉴定均为链格孢菌Alternaria spp.。利用分生孢子液接种橘柚离体嫩叶发现,有22株菌株能侵染橘柚叶片并产生与田间相似的褐斑病症状,确认该病害为链格孢引起的柑橘褐斑病。系统发育树分析结果显示,分离所得的26株菌株均聚在已报道的4个柑橘链格孢进化分支Clade1~Clade4中,其中21株菌株聚在国内特有的分支Clade4中,有3株菌株和1株菌株分别聚在国内外兼有的分支Clade3和Clade1中,1株菌株聚在国外特有的分支Clade2中,表明在福建省采集的这些柑橘褐斑病菌均为链格孢菌,且遗传多样性较丰富。 相似文献
12.
Toshiko Furukawa Kinji Ushiyama Kunihei Kishi 《Journal of General Plant Pathology》2008,74(2):117-119
A leaf spot disease of scarlet sage (Salvia splendens Sellow ex J.A. Shultes) found in Kanagawa and Tokyo prefectures was demonstrated to be caused by Corynespora cassiicola (Berk. and Curt.) Wei based on inoculation experiments, and morphological identification of the pathogenic fungus. Isolates
of C. cassiicola from cucumber, green pepper, and hydrangea were also pathogenic to scarlet sage leaves. Although the isolates from cucumber,
green pepper, and hydrangea were pathogenic to scarlet sage leaves, the scarlet sage isolate was not pathogenic to cucumber,
green pepper, hydrangea, eggplant, tomato or soybean. 相似文献
13.
A new defoliating disease with severe leaf spot of Japanese persimmon (Diospyros kaki) was found in Shimane Prefecture. A fungus, isolated from the ascospores that had formed on the leaf spot, was demonstrated
to reproduce the symptoms on leaves and described as a new species, Adisciso kaki, based on morphological and molecular phylogenetic analyses. This fungus is characterized by small ascomata without clypeus,
obclavate to broadly cylindrical asci with an amyloid apical apparatus, and hyaline ascospores with a submedian septum. We
coined the name black leaf spot (Kurohoshi-rakuyo-byo, in Japanese) for the new disease. 相似文献
14.
15.
Seisaku Umemoto Katsue Nagashima Shigenobu Yoshida Seiya Tsushima 《Journal of General Plant Pathology》2007,73(4):290-292
In 2002, rotted flower clusters and blighted shoot tips and leaves were observed on highbush blueberry (Vaccinium corymbosum L.) and rabbiteye blueberry (V. ashei Reade) plants in Chiba, Japan. The causal fungus isolated from the diseased plants was morphologically identified as Sclerotinia sclerotiorum (Libert) de Bary. The fungus reproduced natural symptoms after inoculation, then reisolated from the symptomatic parts. This
is the first report of blueberry sclerotinia rot caused by S. sclerotiorum.
The nucleotide sequence data reported are available in the DDBJ/EMBL/GenBank databases under accession numbers AB269903#(020501)
and AB233346 (020505). 相似文献
16.
In May 1998 and 1999, two types of leaf spot (black type and brown type) caused by Phoma spp. were found on Farfugium japonicum in Tokyo and in Gunma Prefecture, Japan. The fungus isolated from black-type lesions caused only black-type lesions, and the fungus from brown-type lesions caused only brown-type lesions. We propose to name these diseases black leaf spot of F. japonicum (kokuhan-byo in Japanese) for the disease with black lesions and circular leaf spot of F. japonicum (rinmon-byo in Japanese) for the disease with brown lesions. This is the first report on leaf diseases of F. japonicum caused by Phoma spp. 相似文献
17.
Yoshihiko Hirayama Shunsuke Asano Ken’o Okayama Satoshi T. Ohki Motoaki Tojo 《Journal of General Plant Pathology》2018,84(1):12-19
Colletotrichum fructicola is a major causal agent among anthracnose pathogens of strawberry in Nara, Japan. We hypothesized that a wide range of weeds growing in and around strawberry fields are inoculum sources of the disease and investigated their potential as hosts of C. fructicola. We also examined the influence of herbicide treatment on C. fructicola sporulation on weeds. The fungus was detected on 31 of 541 (5.7%) leaves sampled from 13 weed species from 2005 to 2008. The fungus was most frequently isolated from leaves of Amaranthus blitum with an isolation frequency of 17.9%; inoculation of A. blitum with the pathogen caused brown leaf spots. Other weeds such as Digitaria ciliaris, Galinsoga ciliata, Solidago altissima, Erigeron annuus, and Sonchus oleraceus were found to harbor the fungus at lower rates (4.3–8.1%) without symptoms. C. fructicola formed acervuli on leaves of A. blitum, D. ciliaris, and S. oleraceus after plants were killed by a herbicide (glyphosate). These results demonstrated that infected weeds associated with strawberry cultivation are potential inoculum sources of C. fructicola, especially after herbicide treatment. 相似文献
18.
Masashi Fujinaga Naho Yamagishi Hideki Ogiso Jun Takeuchi Jouji Moriwaki Toyozo Sato 《Journal of General Plant Pathology》2011,77(4):243-247
A new disease was found in Japan, on celery (Apium graveolens var. dulce) having severe chlorotic leaf spot, stunt, and dwarf with leaf curl. A spore suspension from the fungus isolated from affected
plants induced identical symptoms 14 days after plants were sprayed. Identification and molecular characterization showed
that the causal agent is Colletotrichum simmondsii. This report is the first of stunt anthracnose on celery caused by C. simmondsii. We propose the name “stunt anthracnose” for the new disease. Colletotrichum acutatum sensu lato, as reference pathogen of celery anthracnose, should be changed to C. fioriniae based on morphological and molecular characteristics. 相似文献
19.
Song Lisha Lin Wei Jiang Ni Zhang Zhanjiang Tan Guiyu Wei Shugen 《Journal of General Plant Pathology》2021,87(4):259-263
In May 2019, leaf spots were found on Amomum villosum in Guangxi Province, China. Light yellow-green spots first appeared at the tips or edges of leaves. A fungus was isolated from the leaves and identified as Colletotrichum fructicola based on morphological characteristics and molecular identification using the DNA sequences from ITS, TUB2, ACT, and GAPDH sequences. Similar leaf spots developed after leaves were inoculated with the isolate. This is the first report that C. fructicola causes leaf spots on A. villosum.
相似文献20.
E. Sunderasan Rusni A. Kadir Valérie Pujade-Renaud Frédéric de Lamotte H. Y. Yeang Sheila Nathan 《Journal of General Plant Pathology》2009,75(1):19-26
Corynespora leaf disease poses a serious threat to rubber cultivation because infected leaves develop necrotic lesions and
abscise, leaving the tree unproductive. The destructiveness of Corynespora cassiicola has been largely attributed to cassiicolin, a protein toxin secreted by the fungus. Recombinant antibody technology offers
hope to curtail the disease whereby single-chain variable fragments (scFv) specific to cassiicolin could bind and deactivate
the toxin in genetically modified rubber trees that harbour the antibody gene. A scFv phage library was constructed from heavy
and light variable chains of IgG from cassiicolin immunized Balb/C mice spleen. Biopanning of the phage library yielded a
scFv clone with high specificity to cassiicolin. The nucleotide sequence and deduced amino acid sequence information of the
scFv were obtained. Hemagglutinin (HA)-tagged scFv expressed in Escherichia coli is discerned as a band at ca. 30 kDa on SDS-PAGE, and the corresponding band was detected by anti-HA IgG on a Western immunoblot.
Deactivation of cassiicolin by the affinity-purified scFv was demonstrated in a detached-leaf bio-assay on selected susceptible
Hevea clones (PB 260, RRIM 2020, RRIM 901 and RRIM 929). The assay was also performed on clones that are relatively more resistant
to the fungus (RRIM 600 and GT-1), and the results were as expected. Thus, we have successfully demonstrated that the cassicolin-specific
scFv can effectively reduce cassicolin toxicity. 相似文献