Evaluation of haematological, biochemical and histopathological parameters of transgenic rabbits

The aim of our study was to compare the hFVIII mRNA expression in different organs, pathological changes and selected haematological and biochemical blood parameters between transgenic and non-transgenic rabbits from F3 generation. Selected physiological parameters of 3- to 4-month-old transgenic rabbits from F3 generation carrying human factor VIII gene (hFVIII) were analysed and compared with those of non-transgenic ones. Before slaughtering, the blood for haematological and biochemical analysis was taken from the central ear artery. Pathological and histological examination of vital organs and RT-PCR analysis of several tissue organs of transgenic and non-transgenic animals were performed after slaughtering. Except for the mammary gland tissue, slight hfVIII mRNA expression in the spleen, lung and brain and none expression in the liver, kidney, skeletal muscle and heart of rabbits were recorded. pathological examination of vital organs showed some pathological changes in both transgenic and non-transgenic rabbits which were confirmed by histological qualitative evaluations. Statistically significant lower values of blood haemoglobin in blood of transgenic (11.86+/-0.86) animals compared with non-transgenic (12.41+/-1.02, P<0.05) ones and lower parameters of HCT (39.22+/-2.44 versus 40.89+/-2.26, P<0.01) in blood of transgenic rabbits were observed. Parameters of WBC, RBC and PLT showed no significant differences between the analysed groups. All biochemical serum parameters of transgenic rabbits were higher in comparison with non-transgenic ones. Significant differences were found in the concentration of the urea, AST and GMT between transgenic and non-transgenic animals (P<0.001) and in the total protein content, the difference was significant at P<0.05. In conclusion, our results showed that no considerable impact on the general health was found in transgenic rabbits.     

Ultrastructural morphometry of mammary gland in transgenic and non-transgenic rabbits

The mammary gland of transgenic animals has been used for the production of recombinant proteins of therapeutic and nutraceutical use. The objective of this study was to compare the ultrastructure of transgenic and non-transgenic rabbit mammary gland tissue. New Zealand White transgenic rabbits were obtained by breeding non-transgenic rabbits with transgenic founder rabbits containing a whey acidic protein-human factor VIII (WAP-hFVIII) transgene integrated into their genome. Samples of mammary gland tissue from lactating rabbit females were isolated by surgical procedures. These samples were examined by optical and electron microscopy and photographs were taken. Measurements of ultrastructural organelles were made from digital images of the mammary cells. No differences were found in the cellular structure of mammary tissue, but significant differences t((0.001)) in the relative volume of mitochondria and vacuoles between transgenic and non-transgenic mammary gland epithelium were observed.     

转基因牛与非转基因牛性控精液分离及配种效果初探

本研究对转基因牛以及非转基因牛精液经流式细胞仪分离冷冻后精子活力、分离准确率进行了比较,同时对分离的性控冷冻精液进行了人工输精,对受体牛的情期受胎率进行了统计分析。结果表明,转基因牛与非转基因牛精液在冷冻解冻后活力以及分离准确率方面差异不显著(P>0.05);转基因牛与非转基因牛的性控冷冻精液的情期受胎率分别为57.4%、59.3%,二者之间差异不显著(P>0.05)。     

Morphology of Testes from Transgenic Rabbits: Histological and Ultrastructural Aspects

The aim of this study was to compare morphological characteristics of testes from transgenic (the WAP-hFVIII gene) and non-transgenic rabbits with emphasis on the histological and ultrastructural aspects. Samples of testes from both groups were fixed and embedded into Durcupan ACM for transmission electron microscopy. For histological analysis, semi-thin toluidine blue-stained sections were evaluated under a Jenaval light microscope. Male fertility was tested based on egg fecundity and blastocyst yield; transgene transmission was proved using PCR assay. Spermatogenesis in rabbit testes had not been destroyed both in transgenic and non-transgenic rabbits. No significant differences were found in the occurrence of individual cell organelles of the Sertoli cells in transgenic and non-transgenic rabbits. The ultrastructure of Leydig cells in testes of transgenic and non-transgenic rabbits was rather similar. No differences in the occurrence of individual organelles of Leydig cells between transgenic and non-transgenic males were found. These results were in concert with fertilizing capacity of transgenic spermatozoa. The presented status of organelles in this study indicates functional activity of the analysed cells.     

转Bt基因糙米作为日粮原料对生长猪免疫功能的影响

本试验旨在研究转苏云金芽孢杆菌（Bacillus thuringiensis,Bt）基因糙米作为日粮原料对生长猪免疫功能的影响。选用80日龄大白仔猪30头,随机分为2组,每组15个重复,每个重复1头猪。对照组饲喂含70%亲本非转基因糙米的日粮,试验组饲喂含70%转Bt基因糙米的日粮,试验期90 d。结果显示,与对照组相比,饲喂转Bt基因糙米的生长猪血清中IgA、IgG、IgM含量及补体C3和C4水平差异均不显著（P＞0.05）;试验组生长猪的肝脏、脾脏组织切片观察发现,其亚显微结构均无病理变化,与对照组相比无明显差异（P＞0.05）,且两组间的免疫器官指数也均无显著差异（P＞0.05）。综上所述,在本试验条件下,转Bt基因糙米作为生长猪的日粮,未见对生长猪的免疫器官及其功能造成影响。     

Characteristics of Rabbit Transgenic Mammary Gland Expressing Recombinant Human Factor VIII

The objective of this research was to compare (i) the content of milk protein and recombinant human factor VIII (rhFVIII) in the milk of transgenic and non-transgenic rabbit females at three lactations and (ii) histological structure, ultrastructural morphology and occurrence of apoptosis in rabbit transgenic and non-transgenic mammary gland during third lactation and involution. Significant differences (t_0.05) in milk protein content were found between transgenic and non-transgenic at all three lactations. The percentage of apoptotic cells was significantly higher (t_0.01) in non-transgenic ones compared with transgenic mammary gland tissues (6.5% versus 2.4%) taken at the involution stage. Morphometrical analysis of histological preparations at the involution stage detected a significantly higher (t_0.05) relative volume of lumen in transgenic animals compared with non-transgenic ones (60.00 versus 46.51%). Ultrastructural morphology of the transgenic mammary gland epithelium at the involution stage revealed an increased relative volume of protein globules (t_0.05); at the lactation stage, a significantly higher volume of mitochondria (13.8%) compared with the non-transgenic (9.8%) ones was observed. These results, although revealing differences in some parameters of ultrastructure and histology, indicate no harmful effect of the mouse whey acid protein-hFVIII transgene expression on the state of mammary gland of transgenic rabbit females.     

Efficient production of pronuclear embryos in breeding and nonbreeding season for generating transgenic sheep overexpressing TLR4

Background: Brucella is a zoonotic Gram-negative pathogen that causes abortion and infertility in ruminants and humans. TLR4 is the receptor for LPS which can recognize Brucella and initiate antigen-presenting cell activities that affect both innate and adaptive immunity. Consequently, transgenic sheep over-expressing TLR4 are an suitable model to investigate the effects of TLR4 on preventing Brucellosis. In this study, we generated transgenic sheep overexpressing TLR4 and aimed to evaluate the effects of different seasons(breeding and non-breeding season) on superovulation and the imported exogenous gene on growth.Results: In total of 43 donor ewes and 166 recipient ewes in breeding season, 37 donor ewes and 144 recipient ewes in non-breeding season were selected for super-ovulation and injected embryo transfer to generate transgenic sheep.Our results indicated the no. of embryos recovered of donors and the rate of pronuclear embryos did not show any significant difference between breeding and non-breeding seasons(P 0.05). The positive rate of exogenous TLR4 tested were 21.21 % and 22.58 % in breeding and non-breeding season by Southern blot. The expression level of TLR4 in the transgenic sheep was 1.5 times higher than in the non-transgenic group(P 0.05). The lambs overexpressing TLR4 had similar growth performance with non-transgenic lambs, and the blood physiological parameters of transgenic and non-transgenic were both in the normal range and did not show any difference.Conclusions: Here we establish an efficient platform for the production of transgenic sheep by the microinjection of pronuclear embryos during the whole year. The over-expression of TLR4 had no adverse effect on the growth of the sheep.     

转DREB1A/Bar双价基因马铃薯的耐旱性及除草剂抗性分析

在前期获得DREB1A/Bar双价转基因马铃薯的基础上,对转基因植株进行了耐旱性和除草剂抗性分析。耐旱性分析显示,在正常浇水条件下,对照和各转基因马铃薯株系生长状态良好且大致相同,各株系的丙二醛含量、相对电导率和SOD酶活性无显著差异(P0.05)。经过控水10d后,非转基因对照植株叶片明显萎蔫卷曲,而转基因植株仍然保持良好的生长状态;转基因株系的丙二醛含量和相对电导率显著低于非转基因株系(P0.05),而SOD酶活性显著高于非转基因对照(P0.05)。控水18d时,大部分对照植株死亡,死亡率为74.33%;转基因植株只有极少数植株死亡,DR2和DR5的死亡率分别为20.43%和5.65%。用0.3%的市售草铵膦喷施各株系,10d后,对照植株全部枯死,转基因株系的个别叶片干枯,绝大多数叶片及所有茎秆生长状态良好。以上分析表明,DREB1A和Bar基因的导入,明显增强了转基因马铃薯对干旱和除草剂的抗性。     

猪前脂肪细胞为核供体生产表达绿色荧光蛋白的转基因克隆胚胎

分离培养了成年猪前脂肪原代细胞，并对前脂肪细胞向成熟脂肪细胞进行诱导分化，油红O染色法鉴定了脂肪细胞。利用脂质体介导的方法将质粒pEGFP—N1转染前脂肪细胞，经过G418筛选获得了阳性细胞株。然后分别以前脂肪细胞、转绿色荧光蛋白（GFP）前脂肪细胞及胎儿成纤维细胞为核供体进行体细胞核移植，结果表明：前脂肪细胞（8．8％）和胎儿成纤维细胞（8．3％）的囊胚发育率无显差异著（P〉0．05）；与前脂肪细胞相比，转基因前脂肪细胞的囊胚发育率降低，但差异不显著（3．7％ vs．8．8％，P〉0．05）。前脂肪细胞为核供体生产转基因克隆胚胎，能够获得转基因囊胚。虽然囊胚发育率不高，但为生产脂肪细胞转基因克隆猪奠定了基础。     

不同水平柚叶饲粮对生长肉兔血清生化指标、消化和免疫功能的影响

本试验旨在研究不同水平柚叶饲粮对生长肉兔生理机能的影响,进一步评估柚叶作为家兔饲料原料的可行性。选取120只遗传背景相同、健康的35日龄法国伊拉商品兔,适应7 d后,按体重[(1 179.25±53.74)g]相近原则随机分为4组,每组10个重复,每个重复(笼)3只。对照组饲喂不含柚叶的基础饲粮,试验组分别饲喂含6%(Ⅰ组)、12%(Ⅱ组)和18%(Ⅲ组)柚叶的试验饲粮,4种饲粮的能、氮和纤维含量基本相等。试验期28 d。结果发现:1)除Ⅲ组血清球蛋白含量显著高于对照组和Ⅱ组(P0.05),Ⅱ组血清球蛋白含量显著低于Ⅰ组(P0.05)以及Ⅲ组白球比显著低于Ⅱ组(P0.05)外,其余血清生化指标各组间无显著差异(P0.05)。2)饲粮中添加柚叶对生长肉兔消化器官发育指标无显著影响(P0.05)。除试验组后期丙酸比例及Ⅲ组中期盲肠内容物p H和氨态氮浓度显著低于对照组(P0.05)以及Ⅱ组中期p H显著高于Ⅲ组(P0.05)外,其余盲肠发酵参数各组间差异不显著(P0.05)。3)除Ⅲ组血清免疫球蛋白A水平显著低于对照组和Ⅰ组(P0.05)以及Ⅰ组血清免疫球蛋白A水平显著高于对照组和Ⅱ组(P0.05)外,其余免疫指标各组间均无显著差异(P0.05)。由此得出,生长肉兔自由采食含6%~18%柚叶的饲粮4周,不明显负面影响血清生化指标、消化及免疫功能,进一步提示柚叶可作为家兔的饲料原料使用。     

葡萄糖氧化酶对断奶仔兔生产性能和免疫器官发育的影响

试验选用80只35日龄的加利福尼亚断奶仔兔,公母各半。试验兔随机分为4组,每组设5个重复,每个重复4只仔兔。对照组饲喂基础日粮,试验Ⅰ、Ⅱ、Ⅲ组在基础日粮中分别添加0.1%、0.2%、0.3%葡萄糖氧化酶。结果表明,各组间的采食量均无显著差异(P>0.05);试验Ⅱ、Ⅲ组的日增重和料肉比与对照组相比差异显著(P<0.05),但Ⅱ、Ⅲ组之间差异不显著(P>0.05)。试验各组间的胸腺指数和脾脏指数差异均不显著(P>0.05)。由此可见,葡萄糖氧化酶对断奶仔兔的生产性能有一定促进作用。     

过表达FaSAMDC基因提高黑麦草属植物的抗旱性和耐热性

主要探讨了在黑麦草属植物中导入高羊茅S-腺苷甲硫氨酸脱羧酶基因(FaSAMDC)对转基因植株抗旱耐热性的提高效果,为培育黑麦草抗旱耐热新品种提供种质新材料。选择黑麦草成熟种子为外植体,采用贵州省草业研究所分子生物学实验室构建的过表达载体以及黑麦草胚性愈伤组织高频植株再生和农杆菌介导的遗传转化体系,将克隆自高羊茅的FaSAMDC基因转入贵州地区主栽品种多花黑麦草特高和多年生黑麦草四季的基因组内,经抗性筛选、PCR检测和Southern杂交分析验证,获得45株特高与44株四季的转基因植株,转化频率分别为2.93%和2.28%。抗旱耐热试验表明,在30 ℃高温和中度干旱胁迫条件下,特高转基因株系的根冠比比对照组培苗高出7.08%,叶片相对含水量(RWC)比对照高出13.12%,RWC降低幅度比对照少5.49个百分点;四季转基因株系的根冠比比对照组培苗高出6.54%,RWC比对照高出12.54%,RWC降低幅度比对照少6.50个百分点,差异均达到显著水平(P<0.05),证明转入FaSAMDC基因的黑麦草阳性株系的抗旱耐热能力得到了明显提高。形态与生长特征观测结果显示,与非转基因组培苗相比,转基因株系的叶长、株高、主茎节数减小,叶宽、单株分蘖数增加,叶色变深,植株形状趋向于叶片丛生的紧凑型,推测导入的FaSAMDC基因参与了基因表达、细胞分裂等生理功能的调节。     

Effect of dietary supplementation of recombinant Cry and Cp4 epsps proteins on haematological indices of growing rabbits

Genetically modified (GM) crops expressing insect resistance and herbicide tolerance provide a novel approach for improved crop production but their advent at the same time presents serious challenges in terms of food safety. Although prevailing scientific proof has suggested that transgenic crops are analogous to their conventional counterparts, their use in human and animal diet gave rise to emotional public discussion. A number of studies had been conducted to evaluate the potential unintended effects of transgenic crops expressing single transgene, but very few studies for those with multiple transgenes. As the crops with single and multiple transgenes could impart different effects on non‐target organisms, thus, risk evaluation of transgenic crops expressing more than one transgene is required to declare their biosafety. The present study was therefore designed to assess the effects of different levels of dietary transgenic cottonseed expressing recombinants proteins produced by Cry1Ac, Cry2A and Cp4epsps genes on haematological indices of growing rabbits. A total of 48 rabbits were assigned to four dietary treatments containing different levels of transgenic cottonseeds (i.e., 0% w/w, 20% w/w, 30% w/w and 40% w/w) with 0% w/w serving as control. Haematological parameters were measured at periodic intervals (0, 45, 90, 135 and 180) days. No significant (p > 0.05) dose‐dependent effects were observed in most of the haematological parameters evaluated. Though, significant differences (p < 0.05) were recorded in the level of MCHC, MCH and HCT in some of experimental male and female rabbits, yet, they were not biologically significant, as all the differences were within the normal reference values. Our study suggested that feeding transgenic cottonseed of up to 40% could not adversely affect rabbit's haematological profile. However, further study needs to be conducted with different cotton genotypes expressing both single and polygenic traits before recommending the utilization of transgenic cottonseed in routine livestock feeding.     

从光合作用和有机酸积累角度探索转GsPPCK1和GsPPCK3基因苜蓿耐碱性增强的生理机制

光合作用影响着植物的生长发育及产量,并在盐碱胁迫响应中起到积极作用。前期研究将光合途径中野生大豆来源的GsPPCK1和GsPPCK3基因转入苜蓿,所获得转基因苜蓿耐碱性提高。从光合作用和有机酸积累角度探索转GsPPCK1和GsPPCK3基因苜蓿耐碱性增强的生理机制。结果表明,碱胁迫9 d后,叶绿素的相对含量下降,转基因株系P1-5、P3-8与未处理相比变化并不显著,分别下降了11.27%、13.30%,而非转基因株系的叶绿素含量下降了39.11%。转基因株系P1-5、P3-8光合速率(P_n)、蒸腾速率(T_r)、气孔导度(G_s)及胞间CO₂浓度(C_i)在胁迫处理后也有下降趋势,但下降的幅度仅为非转基因植株下降幅度的1/2。光合途径中NADP-苹果酸脱氢酶、NADP-苹果酸酶、磷酸烯醇式丙酮酸羧化酶、丙酮酸正磷酸二激酶、核酮糖-1,5-二磷酸羧化酶等关键酶的酶活及有机酸(苹果酸、柠檬酸、草酰乙酸、磷酸烯醇式丙酮酸)含量在碱胁迫处理后均呈上升趋势,而转基因株系P1-5、P3-8 的光合酶活和4种有机酸含量与非转基因对照相比上升极显著(P<0.01)。PEPC(Medtr4g079860.1)、NADP-ME(Medtr8g014390.1)、NADP-MDH(Medtr1g043040.1)、PPDK(Medtr4g118350.1)及Rubisco(Medtr4g 021210.1)基因的表达呈先上升后下降趋势,转基因株系中基因的表达变化较非转基因对照更为显著(P<0.01)。由此表明,在正常情况下,GsPPCK1和GsPPCK3基因的超量表达并未影响植物的光合作用,但是在碱胁迫下,转基因苜蓿的光合作用受碱胁迫的抑制较小,这一过程可能与PEPC酶的激活有关。另外,光合中间产物有机酸含量的显著上升对维持细胞内pH值的稳定也起到重要作用。     

Sonographic evaluation of thyroid morphology during the normal estrous cycle in the Indo-Pacific bottlenose dolphin (Tursiops aduncus)

Physiologic changes occurring in the thyroid in response to the estrous cycle have been noted in companion animals. However, in bottlenose dolphins, the influence of different reproductive states on thyroid morphology remains unclear. Sonography was used to evaluate the variations of thyroid morphology for nine consecutive estrous cycles of four sexually mature, female Indo-Pacific bottlenose dolphins (Tursiops aduncus). The estrous cycle was divided into two phases for evaluation: the follicular and luteal phases. To compare changes associated with sex, thyroid volumes were measured in the different phases during the estrous cycle of female dolphins and were compared to the ultrasound examinations of the four male dolphins measured over a 2-mo period. In males, there was no significant difference in thyroid volume during the study period (P > 0.05). The thyroid volume measured in combining all estrous cycle phases of the female dolphins was significantly larger than that measured in the male dolphins (P < 0.05). A difference in thyroid volume during the estrous cycle was observed, with the thyroid volume during the follicular phase significantly smaller (P < 0.05) than that of the luteal phase, and is possibly related to the influence of female sex steroids. Thyroid volume variability during estrus should, therefore, be taken into account when examining the thyroid gland of female dolphins.     

转基因糙米对肉仔鸡消化能力及血液生化指标和器官发育的影响

将96只1日龄肉仔鸡随机分成2组,一组饲喂含转基因糙米日粮,一组饲喂含非转基因糙米日粮,试验期42d。测定肉仔鸡18～21日龄和39～42日龄日粮粗蛋白表观消化率和表观代谢能;43日龄心脏采血制备血清,测定其中谷草转氨酶、谷丙转氨酶、碱性磷酸酶、γ-谷氨酰转移酶的酶活性,尿酸以及甲状腺素T3、T4的含量;屠宰后测定器官指数并做显微切片。结果表明:两阶段肉仔鸡日粮蛋白质表观消化率和表观代谢能在2组之间没有差异(P>0.05);血清生化指标在2组之间没有差异(P>0.05);显微镜下观察组织切片没有发现病理变化。结果显示,转基因糙米对肉仔鸡的消化能力和健康状况在试验期内未产生明显的有害影响。     

不同锰源与锰水平对肉仔鸡生产性能和组织矿物元素沉积的影响

为研究不同锰源与锰水平对肉仔鸡的生产性能与组织矿物元素沉积的影响,试验采用2×5完全随机试验设计,在玉米—豆粕型基础日粮(锰含量为37.66mg/kg)中添加0、45、90、130、180mg/kg(5个锰添加水平)的复合氨基酸锰和硫酸锰(2种锰源),构成10个日粮处理组,将540只1日龄健康艾维茵肉仔鸡随机分为10个处理组,开展肉仔鸡饲养试验(共42d)。于35日龄时测定其生产性能指标,计算料肉比;于42日龄时测定其胫骨、肝脏、肾脏组织中铜、铁、锌浓度以及胫骨灰分、钙及磷含量。结果表明,在上述日粮中,锰添加量在45~130mg/kg时,无论是添加复合氨基酸锰组还是添加硫酸锰组35日龄肉仔鸡体重和采食量较未添加组差异显著(P≤0.05),经分析,锰水平对其平均体重及采食量有极显著影响(P<0.01);不同锰源的35日龄肉仔鸡体重、采食量、料肉比差异不显著(P >0.05)。42日龄肉仔鸡胫骨、肝脏、肾脏组织中铜、锌、铁含量及胫骨灰分、钙磷含量未受锰源和锰水平及两者互作的显著影响(P >0.05)。由以上结果可以得出,锰水平对肉仔鸡生长性能有极显著影响(P<0.01);锰源对肉仔鸡生产性能无显著影响(P >0.05);锰源和锰水平及两者互作对肉仔鸡组织矿物元素沉积无显著影响(P >0.05)。     

不同蛋白质水平饲粮对生长期云上黑山羊能量代谢的影响

为研究不同蛋白质水平饲粮对生长期云上黑山羊能量代谢的影响,选取21只体况良好、体重44.84 kg±2.79 kg的处于生长期的云上黑山羊公羊,随机分为3组,每组7个重复,分别饲喂蛋白质水平为9.80%、11.61%和12.66%的3种不同饲粮。所有试验羊进行44 d的饲养试验(预饲期14 d,正试期30 d),在正试期的第12~17天,从3个试验组中各选择健康、体重相近的5只羊进行消化代谢试验。结果表明:①12.66%蛋白质水平组干物质采食量和摄入总能均显著高于9.80%蛋白质水平组和11.61%蛋白质水平组(P<0.05)。②粪能排泄量11.61%蛋白质水平组最低,且显著低于12.66%蛋白质水平组(P<0.05)。③DE、UE、ECH4、ME和NEp三个蛋白质水平组之间差异均不显著(P>0.05)。④总能消化率、总能代谢率3个蛋白质水平组之间差异均不显著(P>0.05);11.61%蛋白质水平组消化能代谢率显著高于12.66%蛋白质水平组(P<0.05)。⑤生长期云上黑山羊的组织和器官能量3个蛋白质水平组之间差异均不显著(P>0.05);除9.80%蛋白质水平组大肠能量显著高于11.61%蛋白质水平组外(P<0.05),其他胃及肠道能量3个蛋白质水平组之间差异均不显著(P>0.05)。综上,说明不同蛋白质水平对云上黑山羊GE、FE、ME/DE有显著影响,饲粮蛋白质水平为11.61%时能量利用率最佳。     

饲用甘露聚糖酶转基因玉米对肉鸡生长性能、屠宰性能及血清生理生化指标的影响

本试验旨在研究甘露聚糖酶转基因玉米对肉鸡生长性能、屠宰性能及血清生理生化指标的影响。试验选择1日龄爱拔益加(AA)肉鸡480只,随机分成4个组,每组6个重复,每个重复20只鸡。4个组安排如下:组Ⅰ为非转基因玉米的对照组,不添加甘露聚糖酶;组Ⅱ为非转基因玉米添加低剂量微生物来源甘露聚糖酶组,甘露聚糖酶活力500 U/kg;组Ⅲ为低剂量甘露聚糖酶转基因玉米组,甘露聚糖酶活力500 U/kg;组Ⅳ为高剂量甘露聚糖酶转基因玉米组,甘露聚糖酶活力5 000 U/kg。试验期42 d。结果表明:与对照组相比,组Ⅲ和Ⅳ42日龄肉鸡平均日增重有显著提高(P0.05),组Ⅳ料重比显著降低(P0.05)。组Ⅳ42日龄肉鸡屠宰率和全净膛率显著高于组Ⅱ和对照组(P0.05)。组Ⅱ、Ⅲ和Ⅳ21和42日龄肉鸡的血液生理生化指标与对照组均没有显著差异(P0.05)。由此可见,饲粮中添加甘露聚糖酶转基因玉米能提高受试肉鸡的生长性能和屠宰性能,但对血液生理生化指标没有显著影响。