无定形氧化铁作为嫌气下NH4+氧化时电子受体的研究

通过¹⁵N示踪试验,以及根据嫌气条件下含¹⁵NH₄⁺培养液加无定形氧化铁后全氮回收量下降(损失¹⁵N约15%),以及产生¹⁵NO₂、¹⁵N₂O、¹⁵N¹⁴NO、¹⁵NO、¹⁵N₂和¹⁵N¹⁴N等含N气体的研究结果,初步证明无定形氧化铁可作为嫌气下NH₄⁺氧化时的电子受体,这可能是引起水田土壤中铵态氮肥损失的又一机理。目前因无各种合¹⁵N氧化物标准气体,无法对其形成的含¹⁵N气体组分进行定量,这一机理对氮素损失的贡献究竟有多大?尚需进一步研究。     

一种直接测定硝化—反硝化气体的15N示踪—质谱法

本文对¹⁵N示踪—质谱法的可靠性进行了检验。结果表明,在不同的¹⁵N丰度气体样品的测定中,用两种方法(反硝化作用源的¹⁵N丰度法和气样的¹⁵N丰度法)计得的反硝化损失量基本一致,故建立起来的¹⁵N示踪—质谱法是可靠的。该方法的测定偏差随气样¹⁵N丰度的降低而增大。此外,回收率结果表明,(N₂+N₂O+NO_x)-¹⁵N累积释放量占加入NO₃-¹⁵N量的94.1％。因此,这一方法可用于直接测定氮肥的硝化—反硝化损失的研究中。     

水稻对氮素的吸收、分配及其在组织中的挥发损失

应用¹⁵N示踪技术研究了水稻不同生育期吸收的¹⁵N在各器官中的分配,以及后期植物组织中的挥发损失。结果发现,水稻在分蘖期吸收的氮量少于在幼穗分化期吸收的氮量;在分蘖期吸收的¹⁵N,标记结束时氮素主要分配于水稻的叶片中,至成熟期¹⁵N有39%转运至水稻子粒中;水稻在幼穗分化期吸收的¹⁵N,标记结束时氮素主要分配在水稻茎和叶鞘中,至成熟期¹⁵N有46%转运至水稻的子粒中;水稻在分蘖期和幼穗分化期吸收的氮素在后期可以通过植株组织挥发损失,至成熟期损失的比例分别达16.7%和13.4%。     

稳定同位素15N的精密质谱测定法

精确地测定生物样品中的微量¹⁵N,是¹⁵N示踪研究法发展的一个基本方向。近年来,在生物学和农业科学中应用稳定同位素¹⁵N取得了很大进展,获得了许多有关动、植物体的氮素营养功能和氮素在土壤中平衡的资料,进一步了解了氮素在自然界的循环过程。     

有机、无机肥料施用后土壤生物量C、N、P的变化及N素转化

研究结果表明,有机、无机肥施用后,土壤微生物量C、N、P开始增加很快,随着时间的推移,土壤微生物量C又有所降低,但生物量N和P则基本保持稳定。硫铵施入土壤后,微生物对肥料¹⁵N的生物固持10天后达到最高峰,以后被固持在体内的¹⁵N有一部分被逐渐释放出来,但一个月后仍有17%左右的¹⁵N被固持在微生物体内。硫铵与有机肥配合施用时,微生物对硫铵¹⁵N固持比例有所增加。有机肥中的¹⁵N被微生物固持的比例也较大,在肥料施入20天左右达到最大值,一个月后仍有19-25%存在于微生物体内。硫铵施用一个月后¹⁵N损失高达18%,有机肥中的N也有少量被损失。     

15N-绿肥和15N-硫铵对土壤有机无机复合体中碳氮的影响

应用¹⁵N示踪技术研究了硫铵和绿肥对土壤碳、氮的影响。结果表明,它们能增加土壤重组碳、氮和紧结态碳、氮,重组中的¹⁵N集中于紧结态腐殖质中,碳、氮的累积量都是施绿肥比施硫铵的为多。各施肥处理的松结态碳、氮在稻季累积,麦季释放,紧结态氮有一部分释放。氮素的下降幅度是松结态氮大于紧结态氮,绿肥处理大于硫铵处理,¹⁵N量大于全氮量。     

Study on Variation in 15N Natural Abundance. Ⅰ. Characteristics of Variation in 25N Natural Abundance of Forest Soils

INTRODUCTION A lot 0fwork has been done on the variation of natural ¹⁵N abundance in soils andtheir N components （Chen et al., 1964）. However, these reports mostly concerned thegeochemical mean of natural ¹⁵N abundance, and only a little work has been done on thecharacteristics of variation of the natural ¹⁵N abundance in different ecosystems and itsrelationship with the properties and conditions of soil.     

不同施用方法下15N标记硫酸铵在稻田土壤中的去向

我们曾在无锡黄泥土上,进行了¹⁵N标记硫酸铵在早稻返青后表施的氮素平衡的研究[1],为了进一步了解在不同施用方法下,¹⁵N标记硫酸铵的氮素平衡情况,又在无锡黄泥土和竖头黄泥土上进行了试验。     

华南受干扰林和成熟林氮素流失对模拟氮沉降的响应

Current nitrogen (N) leaching losses and their responses to monthly N additions were investigated under a disturbed pine (Pinus massoniana) forest and a mature monsoon broadleaf forest in southern China. N leaching losses from both disturbed and mature forests were quite high (14.6 and 29.2 kg N ha^-1 year^-1, respectively), accounting for 57% and 80% of their corresponding atmospheric N inputs. N leaching losses were substantially increased following the first 1.5 years of N applications in both forests. The average increases induced by the addition of 50 and 100 kg N ha^-1 year^-1 were 36.5 and 24.9 kg N ha^-1 year^-1, respectively, in the mature forest, accounting for 73.0% and 24.9% of the annual amount of N added, and 14.2 and 16.8 kg N ha^-1 year^-1 in the disturbed forest, accounting for 28.4% and 16.8% of the added N. Great N leaching and a fast N leaching response to N additions in the mature forest might result from long-term N accumulation and high ambient N deposition load (greater than 30 kg N ha^-1 year^-1 over the past 15 years), whereas in the disturbed forest, it might result from the human disturbance and high ambient N deposition load. These results suggest that both disturbed and mature forests in the study region may be sensitive to increasing N deposition.     

施氮水平对太行山前平原冬小麦-夏玉米轮作体系土壤温室气体通量的影响

应用静态明箱-气相色谱法对4 个施氮肥水平N0 [0 kg(N)·hm^-2]、N200 [200 kg(N)·hm^-2]、N400 [400kg(N)·hm^-2]、N600 [600 kg(N)·hm^-2]的夏玉米-冬小麦季轮作体系2008~2010 年的土壤温室气体(CH₄、CO₂ 和N₂O)排放通量进行研究, 同时观测5 cm 土层土壤温度并记录降水量。结果表明: 太行山前平原冬小麦-夏玉米轮作农田生态系统为CH₄ 吸收汇, CO₂ 和N₂O 排放源。随着氮肥施入量的增加土壤对CH₄ 的吸收速率降低, 而CO₂ 和N₂O 的排放速率增加。冬小麦季施氮处理土壤对CH₄ 的吸收速率显著低于无氮肥的N0 处理, 而N600处理土壤CO₂ 和N₂O 排放速率显著高于N0 处理(P<0.05)。施肥和灌溉会直接导致土壤CO₂ 和N₂O 的排放通量增加, 同时土壤对CH₄ 的吸收峰值减小。土壤温度升高和降水量增加以及干湿交替加剧均会造成N₂O 和CO₂排放速率增加。同时在持续干燥和低温条件的冬季不施氮处理观测到土壤对N₂O 的吸收现象。N0、N200、N400 和N600 处理土壤CH₄ 年排放总量(kg·hm^-2·a^-1)分别为-1.42、-0.75、-0.82、-0.92(2008~2009 年)和-2.60、-1.47、-1.35、-1.76(2009~2010 年), N0、N200、N400 和N600 处理土壤CO₂ 年排放总量(kg·hm^-2·a^-1)分别为15 597.6、19 345.6、21 455.9、29 012.5(2008~2009 年)和10 317.7、11 474.0、13 983.5、20 639.3(2009~2010年), N0、N200、N400 和N600 处理土壤N₂O 年排放总量(kg·hm^-2·a^-1)分别为1.05、2.16、5.27、6.98(2008~2009年)和1.49、2.31、4.42、5.81(2009~2010 年)。     

不同氮源对中国稻田作物氮素营养的贡献

N availability is one of the most important factors limiting crop yield enhancement. The recovery of applications of ¹⁵N-labeled fertilizer and crop residues in a rice-wheat cropping system was determined for up to 6 consecutive growing seasons. The crop residues from the previous season were either incorporated or removed as two different treatments. Our results showed that 16. 55%-17.79% (17.17% on average) of the fertilizer N was recovered in the crop during the first growing season, suggesting that more than 80% of crop N was not directly from the N fertilizer. When ¹⁵N-labeled residues were applied, 12.01% was recovered in the crop in the first growing season. The average recoveries of fertilizer N and crop residue N in the soil after the first growing season were 33. 46% and 85. 64%, respectively. N from soil organic matter contributed approximately 83% of the N in the crop when ¹⁵N fertilizer was applied or 88% when crop residues were applied. There was a larger difference in the total ¹⁵N recovery in plant and soil between N applications in the forms of fertilizer and crop residues. Incorporation of crop residues following the ¹⁵N fertilizer application did not significantly promote ¹⁵N recovery in the crop or soil. On average, only additional 1.94% of N for the fertilizer-applied field or 5.97% of N for the crop residue-applied field was recovered by the crops during the 2nd and 3rd growing seasons. The total recoveries of ¹⁵N in crop and soil were approximately 64.38% for the fertilizer-applied field after 6 growing seasons and 79.11% for the crop residue-applied field after 5 growing seasons. Although fertilizer N appeared to be more readily available to crops than crop residue N, crop residue N replenished soil N pool, especially N from soil organic matter, much more than fertilizer N. Therefore, crop residue N was a better source for sustaining soil organic matter. Our results suggested that the long-term effect of fertilizer or crop residues on N recovery were different in the crop and soil. However, there was little difference between the practices of crop residue incorporation and residue removal following the N fertilizer application.     

种稻下氮素的气态损失与氮肥品种及施用方法的关系

关于水稻田中¹⁵N标记氮肥的氮素去向,国内外都积累了一些资料,并发表了文献综述^[1,2]。近些年来,稻田中氮肥的氮素损失途径的研究又受到了重视^[3-6]。在前两报中^[5,6],我们报导了在石灰性和微酸性水稻土上,碳铵、尿素和硫铵的氮素损失的盆栽和田间试验研究的结果。本次试验的目的主要是探讨两种措施在减少氮素损失中的作用。     

氮、磷、钾配施对葛根生长及品质的影响

为探明氮、磷、钾肥配施对葛根生长及品质的影响,为葛根高效栽培提供合理的施肥技术支撑和理论依据,采用L9(34)正交试验设计,研究氮、磷、钾配施对葛根产量和主要功能成分的影响,明确研究区葛根氮、磷、钾的最佳施用量。结果表明,不同施肥方案对葛根产量和功能成分的影响显著,其中以N3P1K2(N 270kg/hm²,P2O5 45 kg/hm²,K2O 90 kg/hm²)处理的产量和总黄酮含量最高,分别为2.13 kg/ 株和83.10 mg/g;N1P3K2(N 90 kg/hm²,P2O5 135 kg/hm²,K2O 90 kg/hm²) 的葛根素含量最高, 为24.85 mg/g,N1P2K3(N 90 kg/hm²,P2O5 90 kg/hm²,K2O 135 kg/hm²)处理的大豆苷和大豆苷元含量均为最高,分别为22.96 和2.55 mg/g。氮、磷、钾肥配施对葛根产量和葛根素含量影响的大小顺序为氮肥> 钾肥> 磷肥。以葛根产量为目标的最佳施肥量为N 270 kg/hm²、P2O5 135 kg/hm²、K2O 90 kg/hm²,以葛根品质提升为目标的最佳施肥量为N 180 kg/hm²、P2O5 135 kg/hm²、K2O 90 kg/hm²。     

中国亚热带耕作雏形土及强酸土的可蚀性与渗透性关系

To evaluate the validity of different indices in estimating soil readily mineralizable N, soil microbial biomass (Nmic), soil active N (SAN), soluble organic N (SON), net N mineralization rate (NNR) and gross N mineralization rate (GNR) in mineral soils (0-10 cm) from six forest stands located in central Germany were determined and compared with two sampling times: April and November. Additionally, soil density fractionation was conducted for incubated soils (with addition of ¹⁵NH₄-N and glucose, 40 days) to observe the sink of added ¹⁵N in different soil fractions. The study showed that Nmic and NNR in most stands differed significantly (P ≤ 0.05) between the two sampling times, but not GNR, SAN and SON. In November, no close relationships were found between GNR and other N indices, or between Nmic, SON, and SAN and forest type. However, in April, GNR was significantly correlated (P ≤ 0.05) with Nmic, SAN, and NNR along with Nmic under beech being significantly higher (P ≤ 0.05) than under conifers. Furthermore, density fractionation revealed that the light fraction (LF, 0.063-2 mm, > 1.7 g cm^-3) was not correlated with the other N indices. In contrast, results from the incubation study proved that more ¹⁵N was incorporated into the heavy fraction (HF < 0.063 mm, > 1.7 g cm^-3) than into LF, indicaing that more labile N existed in HF than in LF. These findings suggested that attention should be paid to the differences existing in N status between agricultural and forest soils.     

0.01molL-1CaCl2作为土壤不同N素形态浸提剂的研究

本文用荷兰8种表土测试不同温度下0.01molL^-1CaCl₂提取液和淋滤液中N素各形态。试验结果表明温度对NO₃^--N提取量和淋滤量无影响,但对NH₄⁺-N、可溶性总N和还原态N影响显著;可溶性有机态N的释放服从一级动力学方程:N_t=N₀(1-e^-kt),非线性最小二乘法能满意地拟合动力学实验结果。测定0.01molL^-1CaCl₂提取液中的可溶性有机态N对预测土壤N素矿化、合理推荐施肥及防止N素污染可能是一个很有前途的指标。     

华北山前平原农田生态系统氮通量与调控

针对华北太行山前平原冬小麦-夏玉米轮作农田, 研究农田常规施肥[400 kg(N)·hm^-2·a^-1]条件下作物氮素吸收与损失通量过程, 并根据各氮素输出通量特征开展管理调控。研究结果表明, 全年小麦-玉米轮作农田系统氮输入总量为561~580 kg(N)·hm^-2, 输出量468~494 kg(N)·hm^-2, 两季作物总盈余86~93 kg(N)·hm^-2, 其中有机氮为24~36 kg·hm^-2。氨挥发和NO₃^--N 淋溶损失是该区域农田氮素损失的主要途径, 是氮肥利用率低的重要原因。平均每年因氨挥发而造成的肥料氮损失量为60 kg(N)·hm^-2, NO₃^--N 淋溶损失量为47~84kg(N)·hm^-2, 两者占施肥总量的30%。每年因硝化-反硝化过程造成的肥料损失很小, 仅为5.0~8.7 kg(N)·hm^-2。通过施肥后适时灌水、合理调控灌水时间与用量, 以及利用秸秆还田与肥料混合施用等管理措施可改善氮素的迁移和转化规律, 有效减少氨挥发和NO₃^--N 淋溶损失, 并结合缓/控释肥与精准施肥技术, 充分利用土壤本身矿质氮素, 可有效提高养分利用效率和作物产量, 改善农田生态环境与促进农业持续和谐发展。     

华北平原冬小麦-夏玉米轮作体系中标记15N的去向及残效

A field experiment was conducted to investigate the fate of ¹⁵N-labeled urea and its residual effect under the winter wheat (Triticum aestivum L.) and summer maize (Zea mays L.) rotation system on the North China Plain. Compared to a conventional application rate of 360 kg N ha^-1 (N360), a reduced rate of 120 kg N ha^-1 (N120) led to a significant increase (P < 0.05) in wheat yield and no significant differences were found for maize. However, in the 0-100 cm soil profile at harvest, compared with N360, N120 led to significant decreases (P < 0.05) of percent residual N and percent unaccounted-for N, which possibly reflected losses from the managed system. Of the residual fertilizer N in the soil profile, 25.6%-44.7% and 20.7%-38.2% for N120 and N360, respectively, were in the organic N pool, whereas 0.3%-3.0% and 11.2%-24.4%, correspondingly, were in the nitrate pool, indicating a higher potential for leaching loss associated with application at the conventional rate. Recovery of residual N in the soil profile by succeeding crops was less than 7.5% of the applied N. For N120, total soil N balance was negative; however, there was still considerable mineral N (NH₄⁺-N and NO₃^--N) in the soil profile after harvest. Therefore, N120 could be considered agronomically acceptable in the short run, but for long-term sustainability, the N rate should be recommended based on a soil mineral N test and a plant tissue nitrate test to maintain the soil fertility.     

亚铁对水稻土中尿素氮去向的影响

生产实践和研究表明^[1,2],土壤中还原物质积累过多,常是直接或间接导致潜育性水稻土上水稻生长不良和产量较低的原因之一。Fe⁺⁺又是土壤还原物质的主要成分。因此,我们曾于1981年以施用Fe⁺⁺、葡萄糖、紫云英的方法,模拟田间土壤还原条件,进行了水稻盆栽试硷,对潜育性水稻土中N素供应特性及N素平衡作了初步研究^[3]。试验表明,施入亚铁使水稻明显减产,但却促进了水稻对化肥N素的吸收利用,减少了N素的损失。为了进一步摸清和验证施入Fe⁺⁺对水稻土中尿素N素平衡的影响,1982年继续进行了这项工作。     

太湖地区稻麦轮作体系下秸秆还田配施氮肥对水稻产量及经济效益的影响

秸秆还田与配施化肥是未来农业持续发展的方向。为明确秸秆还田条件下获得较高产量和最佳经济效益的氮肥用量, 研究设计了秸秆全量(6 t·hm^-2)还田条件下N0、N1、N2、N3 和N4 5 个氮肥用量的田间试验(肥料N 用量分别为0、120 kg·hm^-2、180 kg·hm^-2、240 kg·hm^-2、300 kg·hm^-2)。两年试验结果表明: 秸秆还田条件下水稻产量随着氮肥用量的增加呈先增加后降低的趋势, 2007 年、2008 年水稻最高产量分别为8 543 kg·hm^-2、7 772 kg·hm^-2, 施氮处理比无氮处理(N0)分别增产9.6%~19.4%、13.0%~17.8%; 当氮肥用量达300 kg·hm^-2 时, 边际产量、氮肥农学利用率、结实率、千粒重、新增纯收益率以及边际成本报酬率均显著低于其余处理(N0~N3), 其中2008 年上述各指标值分别为-4.5 kg·kg^-1、3.0 kg·kg^-1(N)、69.9%、25.1 g、0.91%、1.03 元·元^-1。由水稻产量、经济效益与氮肥用量拟合方程求得最大经济收益时的氮肥用量为218~223kg·hm^-2, 水稻产量和经济收益分别为7 686~8 295 kg·hm^-2 和7 413~8 607 元·hm^-2。因此, 秸秆还田条件下合理配施氮肥, 不仅可以获得最佳经济收益, 还可以获得较高水稻产量和氮肥利用率。     

土壤C/N对苹果植株生长及氮素利用的影响

土壤C/N是土壤氮素循环的重要影响因素。本研究以2年生"富士"/平邑甜茶为试验材料, 应用¹⁵N示踪技术研究了不同土壤C/N[6.21(CK)、10、15、20、25、30、35和40]对苹果植株生长及氮素利用和损失的影响。结果表明: 随着土壤C/N比值的逐渐增大, 苹果新梢长度和植株鲜重均呈先升高后降低的变化趋势, C/N=15、20和25的3个处理苹果新梢长度和植株鲜重最大, 三者间无显著差异, 但均显著高于其他处理。不同C/N处理间植株15N利用率存在差异, 土壤C/N=25时, 植株¹⁵N利用率最大, 为22.87%, 与C/N=20的处理间无显著差异, 但两者均显著高于其他处理; 土壤C/N=40时, 植株¹⁵N利用率最低, 仅为15.43%, 低于CK处理的16.65%。土壤C/N处于15~25时, 植株吸收的氮素来自于肥料氮的比例较高; 而土壤C/N较低(<15)或太高(>25)时, 植株吸收的氮素来自于土壤氮的比例较高。土壤氮素残留量随土壤C/N的增大逐渐增加, C/N=40处理的土壤氮素残留量是CK的1.32倍。随着土壤C/N比值的逐渐增大, 肥料氮损失量呈先减少后增加的变化趋势, 以C/N=25时最少, 仅为施氮量的49.87%, 而对照最大, 为61.54%。因此, 综合土壤C/N对苹果植株生长及氮素平衡状况来看, 土壤C/N为15~25时, 能促进植株的生长发育, 降低氮肥损失, 提高肥料利用率。