我国杨树人工林材性与加工利用研究现状及发展趋势

本文概述了杨树人工林发展概况,全面分析了杨树人工林木材性质与加工利用研究现状和存在问题,指出将杨树人工林材性与加工利用结合起来进行研究是合理、高效利用杨树人工林木材的必由之路。     

湖北省杨树产业化的现状、问题及对策

湖北杨树产业正处于1个快速发展的关键时期,在回顾总结全省杨树产业化现状的基础上,指出了杨树品种的鉴定推广、定向培育目标、杨树抖学研究、管理措施上存在的不足及杨树产业带来的生态问题。并针对这些问题提出了一些相应的对策与建议,旨在促进全省杨树产业的持续快速健康发展。     

洞庭湖区杨树发展对策探讨

通过分析洞庭湖区杨树科研、生产发展现状及存在的问题，提出了进一步发展杨树的战略对策：组建杨树产业集团；建立短周期工业用材定向培育基地；推广优良无性系，建立杨树基因库；加强病虫害综合防治研究；加强杨树综合加工利用的研究与开发。     

抗虫转基因杨树研究进展及前景

随着生物技术的快速发展,杨树抗虫基因工程为害虫综合治理提供了一条新途径。综述了抗虫转基因杨树的研究进展状况,并对杨树抗虫基因工程研究存在的问题和前景进行了讨论。     

杨树基因工程研究进展

运用现代分子生物学技术进行林木遗传改良研究具有重要的现实意义。介绍了杨树基因工程方面的研究进展，综述了杨树遗传图谱构建、基因克隆及转基因应用研究方面的状况，并对杨树基因工程研究存在的问题和前景进行了讨论。总结了杨树基因工程研究的规律，以促进林木基因工程研究的发展。     

通辽市科尔沁区杨树栽培存在的问题及对策

文章阐述了科尔沁区发展杨树的有利条件,分析了杨树栽培方面存在的问题,提出了科尔沁区今后发展杨树应采取的对策。     

渭南市杨树速生丰产林发展现状、存在问题及对策

阐述了陕西渭南市杨树速生丰产林发展的现状、存在问题 ;分析了发展杨树速生丰产林的有利条件 ,提出了加强杨树速生丰产林建设的对策     

杨树农林复合经营研究进展

摘要：杨树农林复合经营是杨树产业的一个重要发展方向,在全球范围逐步受到重视。本文整理了杨树农林复合经营模式、并综述了农林复合经营效益、胁地效应及他感作用等方面的研究进展。在此基础上分析了杨树农林复合经营研究中存在的问题及展望。     

义县地区提高杨树造林成活率的技术措施

义县为杨树适生区,为了解决在长期的营林中存在造林成活率不高、林分生产力低下等问题,该文在阐述义县杨树资源现状和杨树培育中存在问题的基础上,论述提高义县地区造林成活率的技术措施,旨在为该地区杨树人工林发展提供借鉴。     

黄河河套灌区杨树产业可持续发展模式探讨

文章通过对黄河河套灌区杨树资源现状及其产业发展中存在问题的分析,提出了河套灌区杨树产业发展目标和模式。河套灌区具有发展杨树产业的得天独厚的自然条件,但是由于森林自语按培育的市场化程度低,加工增值能力差,社会化服务不健全等问题制约了杨树产业的发展。     

我国意杨加工利用概况

具有与Amur Linden(紫椴)类似的木材性质的意杨已成为我国生产胶合板、细木工板、中密度纤维板和刨花板等人造板的主要原料。在意杨产区形成了专门生产单板的生产基地,向邻近地区的胶合板厂供应单板。由意杨制成的各种胶合板均可达到国家标准规定的性能,近年意杨胶合板已出口韩国、新加坡、日本和美国。     

6个杨树无性系系列对比试验研究

对引进的6个杨树优良无性系系列进行了造林和育苗对比试验。育苗结果表明,6个杨树无性系系列苗木胸径、高度差异极显著,中汉系列苗期表现最优,生长速度顺序为中汉系列、中潜系列、南林95、南林895、351杨、南抗系列。造林结果表明,6年生杨树无性系之间的生长量差异显著,中汉系列、中潜系列、南林95、南林895材积生长表现优异,可在江西省推广应用。     

转果聚糖蔗糖转移酶基因银腺杨的获得

采用农杆菌介导的遗传转化方法,将来自枯草杆菌的果聚糖蔗糖转移酶基因(SacB)导入银腺杨,以提高杨树对水分胁迫的抗性。以来自无菌培养的叶片为外植体,通过大约10 0 0个叶盘与农杆菌LBA4 4 0 4共培养,将植物双元表达载体pKP中SacB基因导入银腺杨基因组,经卡那霉素筛选后,共获得10 2株卡那霉素抗性植株。经PCR特异性扩增和Southern点杂交分析,证明其中97株再生植株基因组DNA中整合了SacB基因。对其中的6 2个无性系进行RT PCR分析,结果表明SacB基因在其中的5 0个无性系中获得表达。温室生长观察表明,转基因无性系外部形态与对照相比没有稳定的显著差异,少数部分转基因无性系的生长明显受到抑制,其他转基因无性系生长正常。这些转基因无性系的获得为培育抗旱转基因杨树奠定了基础。     

Identification of <Emphasis Type="Italic">CpTI</Emphasis> gene integration for 2-year-old transgenic poplars at DNA level

The putative transgenic hybrid triploid poplars [(P. tomentosa × P. bolleana) × P. tomentosa] with CpTI gene have been outplanted in test field for 2 years. Although the authors’ previous studies have proved that they are highly resistant to 3 species of poplar-threatening insect pests and contain high content of CpTI protein in foliage, incorporation status of foreign CpTI gene in poplar genome is uncertain. In this present study, the incorporation of foreign CpTI gene in genome of 5 transgenic poplars was confirmed by PCR and Southern blotting analysis. DNA amplification showed that there were clear DNA bands of about 450bp specific to CpTI gene in transgenic lanes, while no corresponding band in non-transgenic lane was observed. Correspondingly, clear DNA hybridization signals and no signal were exhibited on film for DNA Southern blotting analysis in transgenic lanes and non-transgenic lane, respectively, which further confirmed the stable integration of foreign CpTI gene in genome of 2-year-old transgenic poplar.     

杨树短周期纤维板工业用材林定向培育研究

围绕杨树短周期纤维板工业用材林培育所需的优良无性系、造林密度等关键技术开展研究,结果表明：中潜、中汉、南抗无性系列之间差异显著;试验设计造林密度为3 m×3 m、2.5 m×4.5 m和3 m×4 m,6年生时,2.5 m×4.5 m蓄积量最高,达139.9 m3/hm2,投入产出比为1∶4.5。     

ACC氧化酶cDNA克隆及其对美洲黑杨体内乙烯产生的反义抑制

利用ＲＴ－ＰＣＲ技术克隆了番茄ＡＣＣ氧化酶基因ＬＥＥＴＨＹＢＲ编码区０．９ｋｂ的ｃＤＮＡ片段,经酶切图谱分析和序列分析鉴定后,反向插入到植物表达载体ｐＢｉｎ４３８中,构建了表达ＡＣＣ氧化酶反义ＲＮＡ的二元载体。用农杆菌侵染美洲黑杨叶片,在含卡那霉素的ＭＳ培养基上选择转化子和植株再生,通过ＰＣＲ检测筛选到１６株转基因杨树植株,Ｓｏｕｔｈｅｒｎｂｌｏｔ分析初步确证了外源基因是以单拷贝插入到杨树基因组中;对杨树幼苗乙烯释放量的测定结果表明转基因杨树的乙烯释放量为对照植株的２８％。     

黑杨派杨木特性及其胶合板制造工艺

介绍了洞庭湖区引种的黑杨派南方型杨木的特性,根据原料特性设计了胶合板制造工艺并对工艺参数进行优选。试制结果表明,采用文中提供的工艺及参数,可制得符合要求的速生杨木胶合板。     

Identification of CpTI Gene Integration for 2-year-old Transgenic Poplars at DNA Level

The putative transgenic hybrid triploid poplars [(P. tomentosa P. bolleana) P. tomentosa] with CpTI gene have been outplanted in test field for 2 years. Although the authors previous studies have proved that they are highly resistant to 3 species of poplar-threatening insect pests and contain high content of CpTI protein in foliage, incorporation status of foreign CpTI gene in poplar genome is uncertain. In this present study, the incorporation of foreign CpTI gene in genome of 5 transgenic poplars was confirmed by PCR and Southern blotting analysis. DNA amplification showed that there were clear DNA bands of about 450bp specific to CpTI gene in transgenic lanes, while no corresponding band in non-transgenic lane was observed. Correspondingly, clear DNA hybridization signals and no signal were exhibited on film for DNA Southern blotting analysis in transgenic lanes and non-transgenic lane, respectively, which further confirmed the stable integration of foreign CpTI gene in genome of 2-year-old transgenic poplar.     

Control tactics of poplar diseases in China

Introduction9Withtherapidlydevelopingofartificialpoplarforests,poplardiseasesincreasedgradually.Specially,poplarlonghornedbeetlesandcankerhavebecomethemainharmfulfactorsofaffectingthedevelopmentofpoplarstands.Therefore,thecontroltacticsforpoplarDothiorellacankerandCytosporacankerhavebeenlistedforkeyprojectinSeventhFive-YearPlan,EighthFive-YearplanandNinthFive-YearplanofChinasincethemiddleof80s.InTwentiethInternationalPoplarConference,"IntegratedcontroltechniqueofpoplardiseaseinChina"as…     

Transgenic poplar with enhanced growth by introduction of the ugt and acb genes

The transgenic poplar (Populus tremula L.) was obtained by transfer of the ugt and acb genes via triparental mating, which was employed to deliver large fragments of TDNA as a cluster. Freshly harvested seeds of local poplar were placed on MS agar medium and plantlets were obtained. After 1 year of subcultivation, plantlets were infected with a transconjugant of triparental mating with target ugt and acb genes into axillary buds. The transformed sprouts so obtained were cut and subcultivated on agar medium with an addition of 0.6 mg/l indole-3-butyric acid as an auxin source. The transformed sprouts showed GUS activity and resistance to gentamycin and kanamycin. The integrity of the target ugt and acb genes into poplar genome was demonstrated via PCR and Southern blot hybridisation. The transgenic poplar plants revealed a higher growth energy, corresponding to a higher content of IAA as opposed to control plants. Both transgenic and non-transformed plants were potted into soil for outdoor acclimatisation and subsequently transferred to earth in beds. Growing outside during 3 years, the transgenic poplar demonstrated a higher growth rate with fast bud and branch development.