超临界CO2萃取斜叶黄檀精油工艺优化及精油成分分析

本研究采用超临界CO2萃取斜叶黄檀中的精油,并分析精油的挥发性成分,主要考察萃取压力、萃取温度和萃取时间对精油得率的影响,通过正交试验确定超临界CO2萃取精油的最佳工艺条件,并通过气相色谱-质谱联用（GC-MS）检测斜叶黄檀精油中的主要挥发性成分。结果表明:超临界CO2萃取精油的最佳工艺条件为萃取温度75℃,萃取压力35 MPa,萃取时间4 h,在此条件下精油的得率为4.7573%;从斜叶黄檀精油中共鉴定出13种挥发性组分,主要成分是榄香素和甲基丁香酚,分别占92.33%和3.18%。      

金柑果皮精油超声波辅助提取工艺优化及其抑菌、抗氧化活性

采用响应面法对金柑果皮精油超声波辅助提取提取工艺条件进行优化,并对金柑果皮精油的抑菌和抗氧化活性进行评价。结果表明,金柑果皮精油超声波辅助提取的最佳工艺条件为:液料比27∶1 m L/g、超声时间1.25 h、超声波功率300 W。在此条件下,金柑果皮精油得率为2.24%,与理论预测值基本一致。金柑果皮精油对金黄色葡萄球菌、大肠杆菌、沙门氏菌、黑曲霉、酵母菌均有一定抑制作用,最小抑菌浓度分别为1.0%、0.5%、0.5%、0.5%、0.1%。抗氧化活性结果表明,金柑果皮精油对食用油脂的货架期有一定的延缓作用,具有一定的清除羟自由基、DPPH自由基的能力和总抗氧化能力。     

超临界CO_2提取人参籽油工艺研究及人参籽油脂肪酸组成分析

该研究以人参籽为原料,采用超临界CO2流体技术萃取人参籽油,通过正交试验优化了萃取人参籽油工艺参数,再将其与通过冷榨法及溶剂法提取的人参籽油进行对比,测定和比较了不同工艺制取的人参籽油的脂肪酸组成。试验结果表明,在萃取压力26MPa、萃取温度50℃、萃取时间2 h工艺条件下,人参籽油得率为16.2%。经检测,人参籽油脂肪酸组成为不饱和脂肪酸占99%以上,其中油酸含量极高,可达73.93%。通过不同制取方法所得到的人参籽油的脂肪酸组成分析表明,超临界CO2萃取法与溶剂萃取法所提取人参籽油的脂肪酸明显多于压榨法的脂肪酸成分;且超临界CO2萃取法具有无有机化学试剂残留的明显优势,因此,是未来人参籽油提取中可以应用的一种绿色环保的提取工艺和技术。     

荔枝果皮精油的超临界萃取研究及精油成分分析

荔枝果皮精油的超临界ＣＯ２萃取研究及精油成分分析的实验结果表明,荔枝果皮粉精油的最佳萃取条件为： １２ ＭＰａ,３５℃, １～１．５ ｈ,当含水量为 ６％时萃出率较高。采用气相色谱－质谱联用技术进行精油成分分析,鉴别出６０多种成分。     

超临界CO2萃取人参挥发油工艺的研究

目的确定人参挥发油超临界CO2萃取的最佳条件。方法通过超临界CO2萃取人参挥发油的单因素试验和正交试验,研究其萃取工艺条件,考察萃取压力,萃取温度,萃取时间,CO2流量对挥发油得率的影响。结果确定了超临界萃取人参挥发油的最佳条件:萃取压力30MPa,萃取温度40℃,萃取时间100min,流量25~30L/h。结论该条件下人参挥发油(粗油)的得率为0.880%,远远高于传统索氏法提取的人参挥发油得率。     

葵花子油不同提取工艺的比较

通过比较超临界CO3提取葵花子油的均匀设计实验和微波、超声波诱导提取葵花子油的正交实验结果,考察影响提取的主要因素,寻求最佳萃取工艺.超临界CO2萃取最佳工艺条件为:压力32MPa,温度32℃,CO2流量25kg/h,萃取时间80min,得率36.45%;微波萃取最佳工艺条件为:溶剂为正己烷,物料质量与溶剂质量比例1:3,辐射时间7min,辐射功率720w,得率27.12%.超声波萃取最佳工艺条件为:物料质量与溶剂质量比例1:7,溶剂为正己烷,浸泡时间18h,得率17.37%.结果表明超临界CO3萃取葵花子油品质最好,而且萃取也最高,质量最稳定.     

超临界CO2萃取金柑籽油工艺

研究了超临界CO2萃取金柑籽油的最佳工艺,考察了萃取时间、萃取压力、萃取温度、CO2流量、解析压力、解析温度对萃取效果的影响。试验表明,在萃取温度为40℃、萃取压力为30 MPa、解析压力为10 MPa、解析温度为55℃和CO2流量为15 L·h^-1的条件下萃取率可达45.1%。     

响应面优化超声强化超临界CO_2萃取大豆胚芽油工艺

研究超声强化超临界CO2萃取大豆胚芽油的提取工艺,通过响应面分析法优化得到的萃取条件为:萃取时间为138 min,萃取温度为37℃,萃取压力为26.3 MPa,超声波功率为300 W,超声波时间为29 min.在此条件下大豆胚芽油萃取率为87.24%左右.且大豆胚芽清油的颜色浅黄透明,香气纯正.     

23种植物精油对橘小实蝇的行为反应研究

通过超临界 CO2 萃取技术萃取 23 种植物精油,并使用 Y 型嗅觉仪测试了 23 种植物精油对橘小实蝇雌虫的行为反应。 结果表明,当精油质量浓度为0.5％时,在5min内,番石榴果精油、黄皮果皮精油、莲雾叶精油对橘小实蝇雌成虫有明显的引诱效果;香蕉叶精油、大叶桃花心木精油、番石榴叶精油对橘小实蝇雌成虫有明显的驱避效果。     

姜油树脂的超临界CO_2萃取条件及其抑菌活性

采用正交试验,优化了超临界CO2萃取姜油树脂的工艺条件,在压力22 Mpa,时间15 min,温度50℃,萃取率可达5.38%。抑菌试验结果表明,姜油树脂对大肠杆菌、沙门氏菌、痢疾志贺菌、苏云金杆菌和金黄色葡萄球菌均有一定的抑制作用,抑菌最低质量浓度为0.5～1.0 mg/mL。在不同温度或不同时间紫外光照条件下,姜油树脂仍有一定的抑菌作用,表明其具有一定的光、热稳定性。     

Analysis of nutritional components of eight famine foods of the Republic of Niger

In the western Sahel, indigenous plants become important staples when cereal harvests are inadequate to support populations inhabiting that region of Africa. The purpose of this study was to assess the nutrient content of several of these edible wild plants. The leaves of the following seven plant foods were analyzed: Ziziphus mauritiana, Cerathotheca sesamoides, Moringa oleifera, Leptadenia hastata, Hibiscus sabdarifa, Amaranthus viridi, and Adansonia digitata. The fatty acid, vitamin E, carotenoid, selected mineral and amino acid contents of these plant foods were determined. These same analyses were performed on the fruit of the Adansonia digitata. In quantitative and qualitative terms, Amaranthus viridis was found to be an excellent source of protein. Its amino acid composition compared favorably to that of a World Health Organization (WHO) protein standard. It also contained considerable amounts of the two fatty acids that are essential in humans (linoleic and -linolenic) and a number of minerals including iron, magnesium, calcium and zinc. The leaves of Hibiscus sabdarifa contained an appreciable quantity of protein the composition of which was comparable to the WHO standard. The mineral content of the leaves of this plant was also exceptionally high; noteworthy was its high zinc content. H. sabdarifa also contained significant quantities of the two essential fatty acids. Ziziphus mauritiana was an excellent source of the essential fatty acid linoleic acid and several of the metals including iron, calcium, magnesium and zinc. Its content of other essential nutrients, however, was rather low. In general, Adansonia digitata leaves were nutritionally superior to the fruit of the tree; however, the fruit did contain useful quantities of potassium, phosphorus, zinc and -linolenic acid. The Leptadenia hastata leaves were an especially good source of lutein and -carotene. These data should be useful to the people who inhabit the western Sahel in helping them devise healthy diets during times when cereal staples are in short supply.     

狗牙根诱变后代抗寒性评价

通过电解质外渗法和匍匐茎恢复试验对‘阳江’狗牙根及其12个通过形态鉴定选出的坪用价值高且花序密度低的诱变后代进行抗寒性鉴定。电解质外渗法结果表明:诱变后代间的抗寒性具有较大差异,其叶片半致死温度(LT₅₀)的变异范围为-7.6～-0.2℃(最低值与最大值相差7.4℃);参试材料抗寒性由强到弱依次为M18>M4>M26>M28> M22>阳江>M29>M31>M10>M37>M16>M1>M25,其中,有5个诱变后代抗寒性优于亲本,分别是M18、M4、M26、M28、M22。匍匐茎恢复实验结果表明:诱变后代M1、M22、M26、M31、M25在0℃和-5℃低温胁迫后的恢复生长率都高于亲本,恢复能力均优于亲本;M10、M37、M28在-5℃低温胁迫下,恢复生长能力低于亲本,抗寒性相对较弱,M16和M4在0℃和-5℃低温胁迫下,恢复生长率都低于亲本,抗寒性明显弱于亲本。综合2种方法鉴定结果显示:诱变后代M1、M25的恢复能力较强;M4、M28的叶片抗寒性较好,青绿期较长;M22、M18、M26的叶片抗寒性和匍匐茎恢复能力均较强;M29,M31的叶片抗寒性和匍匐茎恢复能力与亲本相似;M10、M16、M37的叶片抗寒性和匍匐茎恢复能力均较弱,整体抗寒性较弱。     

芦笋枯萎病病原鉴定

对造成福建省漳州市东山县的芦笋枯萎病病原菌进行分子鉴定,以期确定病原菌的属、种名,为防治该病害寻求理论依据。试验对分离纯化的病原菌核糖体DNA的ITS区进行测序,在Genbank中搜索其同源性并构建它们的系统发育树,结果表明：引起芦笋枯萎病的病原菌是镰刀属中的尖孢镰刀菌(Fusarium oxysporum),且病原菌之间的同源性高达89%,遗传差异不显著。     

橡胶树丛枝病病原的抗血清制备与应用

以感染橡胶树丛枝病病原的长春花为材料,制备得到橡胶丛枝病病原菌抽提液,以抽提液为抗原免疫家兔,制备抗血清,经微量沉淀测定,抗血清效价为１：２０４８。应用橡胶丛枝病抗血清检测橡胶褐皮病,无症苗木检出率达３０％－３７％,可疑的褐皮病树检出率达８５．７％。     

Size and frequency of occurrence of pre-roost gatherings of starlings

One hundred and fifty-six pre-roost gatherings of starlings were observed at 28 sites around a roost in west Norfolk during winter. Pre-roost gatherings occurred more frequently at individual farmyards compared with individual fields, but 82% of the sites where gatherings occurred were on fields. The size of gatherings was greater, the closer to the roost. Birds in pre-roost gatherings on fields of autumn-sown winter wheat spent most of the time feeding and had a diet composed almost exclusively of wheat seeds. Birds in gatherings on grass fields also fed intensively whereas only 31% of birds at farmyards were feeding. The implications that these results have with regard to potential damage to fields of winter wheat and around farmyards are discussed.     

农杆菌注射法瞬时转化荔枝果实组织的研究

为了寻找一种高效的荔枝果实瞬时基因表达方法,本研究以荔枝品种‘新球蜜荔’（Litchi chinensis Sonn. var. ‘Xinqiumili’）为试材,利用农杆菌注射法对荔枝果实组织进行转化,研究了果实发育时期、菌株种类、注射部位、取样时间、菌液浓度等对转化效率的影响。结果表明：选择果肉已完全包裹种子的Ⅱb期果实进行连体注射,在果柄、果皮、种子、果肉分别注入OD₆₀₀值为2.4的农杆菌菌株GV3101,4 d后取样进行检测,4个组织的GUS染色率较高。本研究成功建立了适用于荔枝果实的基因瞬时表达系统,为今后快速鉴定荔枝果实相关基因功能提供了基础。     

《生物安全法》实施背景下基因编辑技术的安全评价与监管

2020年10月17日,《中华人民共和国生物安全法》(以下简称《生物安全法》)通过全国人大常委会的审议,自2021年4月15日起施行.生物技术的研究与应用安全是《生物安全法》涉及的主要内容之一,基因编辑作为近几年生物技术领域的研究热点,其安全性评价和监管备受关注.本文概述了基因编辑技术的应用现状,比较了不同国家对基因编...     

蝉拟青霉疏水蛋白PChyd基因表达模式分析及敲除载体构建

为深入研究昆虫病原真菌蝉拟青霉疏水蛋白PChyd基因的功能,根据蝉拟青霉基因组信息克隆疏水蛋白PChyd基因,对该基因序列进行生物信息学分析,使用qRT-PCR技术对其在不同培养条件或阶段下的表达模式进行分析,并通过酶切酶连的方法构建了该基因的敲除载体。结果表明：PChyd基因的开放阅读框序列全长303 bp,编码100 aa,包含22 aa的信号肽序列和70 aa疏水蛋白功能区域。系统发育分析显示该基因与粗糙虫草菌亲缘关系最近。qRT-PCR结果显示PChyd基因在PDA培养的菌丝体、诱导的附着胞、诱导的芽生孢子中表达量显著高于另外2个样品,其中芽生孢子表达量最高,暗示该基因在蝉拟青霉侵染初期和在昆虫血腔中定殖阶段可能具有重要作用。凝胶电泳结果表明,成功构建了该基因的敲除载体,扩增出含有上臂、HPH、下臂的3356 bp左右的片段。本研究为进一步探究蝉拟青霉疏水蛋白PChyd基因的致病机理、生防工程菌的改造奠定了基础。     

波罗蜜叶片突变体叶绿素含量测定和超微结构观察

波罗蜜（Artocarpus heterophyllus）是一种热带果树,至今对其突变体的研究少有报道。本研究以波罗蜜叶片为试验材料,探究其叶片出现白化和返绿现象的可能原因。（1）用分光光度计法、比色法测定波罗蜜叶片突变体和正常绿叶的叶绿素及叶绿素前体物质含量;（2）用叶绿素酶Elisa试剂盒测定叶绿素酶活性;（3）用透射电子显微镜对叶绿体的超微形态结构进行观察。研究结果表明：（1）不同叶色所产生的叶绿素a含量、叶绿素b含量、叶绿素总含量、类胡萝卜素含量均存在显著差异;（2）波罗蜜叶片突变体和正常绿叶的叶绿素合成前体物质含量之间并没有出现显著性差异;（3）对波罗蜜叶片的叶绿素酶活性进行测定时,发现其活性出现显著差异,但对其叶绿素含量的差异性并没有产生较大影响;（4）观察波罗蜜叶片内叶绿体超微形态结构时,发现正常绿叶的叶绿体形态完好且数量较多,白化叶和返绿叶的叶绿体内部结构存在缺陷,其原因是叶绿体基粒构建阶段受阻;基于测定波罗蜜叶片中的叶绿素、叶绿素前体物质含量和叶绿素酶活性,并观察波罗蜜叶片内叶绿体的超微形态结构,对得到的数据结果进行比较分析。本研究推测是在叶绿素合成阶段,原脱植基叶绿素合成叶绿素时受阻及叶绿体发不良导致波罗蜜出现白化和返绿现象,为今后进一步综合研究波罗蜜突变体提供理论依据。     

Mechanism of action of insecticidal secondary metabolites of plant origin

Insect pest management is facing the economic and ecological challenge worldwide due to the human and environmental hazards caused by majority of the synthetic pesticide chemicals. Identification of novel effective insecticidal compounds is essential to combat increasing resistance rates. Botanicals containing active insecticidal phytochemicals appear to be promising to address some of these problems. Therefore, there is a continuous need to explore new active molecules with different mechanisms of action. Secondary metabolites present in plants apparently function as defense (toxic), which inhibits reproduction and other processes. The phytochemical biomolecules could be used for maximizing the effectiveness and specificity in future insecticide design with specific or multiple target sites, while ensuring the economic and ecological sustainability. In this article, the current state of knowledge on phytochemical sources and insecticidal activity, their mechanism of action in insects, resistance, and promising advances made in phytochemical research are reviewed.