Spatial patterns and drivers of plant diversity in the tropical city of Sanya,China

Drivers of patterns in plant diversity remain poorly understood in tropical cities. Therefore, we investigated diversity within the tropical city of Sanya in Hainan Province of southern China by sampling one to three plots within 154 urban functional units (UFUs) based on six primary and 18 secondary UFUs. To measure diversity, we determined the number of vascular plants (species richness; SR) within each functional unit based on vegetation plots. We computed Faith's phylogenetic diversity (PD_Faith) using a large megatree of plant life. To assess the potential drivers of SR and PD_Faith, we performed multiple regressions using socioeconomic, biophysical, and vegetation management variables. The results showed significant differences in SR and PD_Faith among the six primary UFUs. PD_Faith of cultivated species was significantly higher than PD_Faith of spontaneous species in all UFUs except in the wasteland area. Management measures were better predictors of SR and PD_Faith than socioeconomic variables. The best predictor of SR and PD_Faith of spontaneous species was the management of the urban green space. Our results provide clear guidelines for improved management strategies in Sanya, especially by showing how spontaneous and/or cultivated plant diversity can be encouraged depending on environmental, ecological, and cultural needs. Our work also adds to the presently scarce literature on drivers of urban plant diversity in tropical cities worldwide.     

Regeneration Strategies,Disturbance and Plant Interactions as Organizers of Vegetation Spatial Patterns in a Pine Forest

To determine how vegetation pattern in early successional forests may be related to plant traits and types of disturbance, we measured percent cover of individual taxa annually in a South Carolina Pinus elliottii forest, starting one year before, and ending four years after harvest and tree girdling disturbances were applied. The 17 most important taxa surveyed were grouped into four regeneration strategies chosen a priori, and the spatial patterns of these groups and of the soil were investigated using global variability, semivariograms and kriged maps. We also examined spatial correlations across years, across taxa, and between species and soil disturbance. Seed bank taxa represented by Dichanthelium spp. increased rapidly and formed large patches, and then quickly declined. Taxa that regenerate by newly dispersed seeds, represented by Rhus copallina and Rubus spp. occurred at first in a few patches, and became widespread later. Stump sprouters, represented by Quercus spp. and Myrica cerifera, had rapid increases in cover, but their spatial patterns were largely determined by their pre-disturbance patterns. Prunus serotina, which relies on both sprouting and dispersed seed, had moderate cover and a random distribution. Within-species temporal correlation of spatial pattern was lower in girdled than in harvested plots, and was not clearly related to regeneration strategy. Forest floor disturbance was patchy and affected the pattern of Dichanthelium spp. in the harvested plots. Negative correlations between herbs and woody plants in harvested plots reflected the role of biotic (i.e., successional) filters on vegetation pattern. Surprisingly, no spatial correlations were detected between the nitrogen fixer, Myrica cerifera and other taxa in this N-limited system. In comparing the spatial and temporal patterns, we found kriged maps more informative than analysis of semivariograms alone. The maps and correlation statistics demonstrated that regeneration traits, spatial patterns of soil disturbances, and interactions among taxa influence dynamics of the spatial patterns of the plants. We also demonstrated that disturbance types affected the importance and interactions among these three factors, and caused different spatial patterns of the plant taxa.     

Integration of invasion ecology theories into the analysis of designed plant communities: a case study in Southern Germany

Theories regarding the establishment and persistence of self-naturalising alien species can help in interpreting these processes in designed plant communities with their combination of exotic species and native plant communities. Thus, they may provide a theoretical basis for this kind of landscape design. A case study investigated the influence of plant community conditions (invasibility), species-specific traits (invasiveness), and gap diameter size on the establishment of selected North American prairie forbs in Central European horticultural meadows. Experimental sites were located in Freising, Bavaria. Introduced forbs included Aster laevis, Aster novae-angliae, Aster x salignus, and Aster x versicolor. Establishment success was measured as survival rate and total aboveground dry biomass. Invasibility of the investigated horticultural meadows was strongly related to resource availability, as most influences of plant community traits could ultimately be attributed to this factor. Leaf area and specific leaf area above canopy height of the resident meadow species appear to be the traits that best explained differences in establishment success of the Asters. Gap size influenced species performance mainly on the less productive site, again due to higher availability of resources in the larger gaps. These results are consistent with findings of studies on self-naturalising alien species. By applying this interdisciplinary approach, valuable insights in the functioning of designed plant communities could be gained. Horticultural meadows can be one important tool in designing the highly dynamic urban landscape. In choosing suitable sites, resource availability should be strongly considered.     

Combining endophytic fungi and bacteria for the biocontrol of Radopholus similis (Cobb) Thorne and for effects on plant growth

Eight endophytic fungal and bacterial isolates with antagonistic activity against Radopholus similis were evaluated in vivo for their individual and combined effects on biocontrol of R. similis and on the growth of “Grand Naine” cultivar banana plantlets in the greenhouse. Penetration efficiency (PE) of R. similis was between 3 and 21% in 29 biological agents (BAs) treatments, less than the 29% of the nematode-alone control (p ≤ 0.0001); 24 of the BAs treatments did not differ from the PE of 5% for a nematicide control. Twenty nine BAs treatments exhibited antagonistic activity against nematodes which reduced final population levels between 18 and 93%, relative to those on nematode-alone control plants (p ≤ 0.0001), and 14 BAs treatments were statistically similar to the nematicide treatment (88% reduction). Twenty four BAs treatments had increments of plant root biomass ranging from 20 to 58%, greater than the control plants; 37% of the treatments with single and combined BAs inoculations had root length increments ranging from 29 to 54% compared with control and chemical treatment. The nematicide, Terbufos 10GR, did not affect plant growth.     

Post-storm surveys reveal large-scale spatial patterns and influences of site factors,forest structure and diversity in endemic bark-beetle populations

The storm that struck France on december 26^th and 28^th 1999 felled 140 million m³ of timber and had a high economic, social and landscape impact. This event offered the opportunity to study large-scale patterns in populations of forest insect pests that would benefit from the abundant breeding material. A large-scale survey was carried out in France in 2000 to sample the most frequently observed species developing on spruce (Ips typographus, Pityogene schalcographus) and pine (Tomicus piniperda, Ips sexdentatus) in 898 locations distributed throughout wind-damaged areas. The local abundance of each species scored on a 0 to 5 scale was analysed using geostatistical estimators to explore the extent and intensity of spatial autocorrelation, and was related to site, stand, and neighbourhood landscape metrics of the forest cover (in particular the interconnection with broadleaf forest patches) found within dispersal distance. All species but I. sexdentatus, which was much less abundant, displayed large-scale spatial dependence and regional variations in abundance. Lower infestation levels per tree (windfalls and standing trees) were observed in stands with a high proportion of wind-damaged trees, which was interpreted as the result of beetles distributing themselves among the available breeding material. More infestations were observed in wind-broken trees as compared to wind-felled trees. More importantly, populations showed significant relationships with the structure of coniferous stands (in particular with the number of coniferous patches). T. piniperda population levels were negatively correlated to the amount of coniferous edge shared with broadleaf forest patches, possibly because of the disruptive effect of non-host volatiles on host-finding processes at the landscape-scale. The differences observed between species regarding patterns and relationships to site, stand, and forest cover characteristics are discussed in relation to the ecological characteristics of each species.     

Spatial Patterns in European Rabbit Abundance After a Population Collapse

Assessing the associations between spatial patterns in population abundance and environmental heterogeneity is critical for understanding various population processes and for managing species and communities. This study evaluates responses in the abundance of the European rabbit (Oryctolagus cuniculus), an important prey for predators of conservation concern in Mediterranean ecosystems, to environmental heterogeneity at different spatial scales. Multi-scale habitat models of rabbit abundance in three areas of Doñana, south-western Spain, were developed using a spatially extensive dataset of faecal pellet counts as an abundance index. The best models included habitat variables at the three spatial scales examined: distance from lagoons (broad scale), mean landscape shrub coverage and interspersion of pastures (home-range scale), and shrub and pasture cover (microhabitat scale). These variables may well have been related to the availability of food and refuge for the species at the different scales. However, the models’ fit to data and their predictive accuracy for an independent sample varied among the study regions. Accurate predictions in some areas showed that the combination of variables at various spatial scales can provide a reliable method for assessing the abundance of ecologically complex species such as the European rabbit over large areas. On the other hand, the models failed to identify abundance patterns in a population that suffered the strongest demographic collapse after viral epidemics, underlining the difficulty of generalizing this approach. In the latter case, factors difficult to implement in static models such as disease history and prevalence, predator regulation and others may underlie the lack of association. Habitat models can provide useful guidelines for the management of landscape attributes relevant to rabbits and help improve the conservation of Mediterranean communities. However, other influential factors not obviously related to environmental heterogeneity should also be analyzed in more detail.     

Development of a male sterile eggplant by utilizing the cytoplasm of Solanum virginianum and a biparental transmission of chloroplast DNA in backcrossing

To develop a male sterile line of eggplant (Solanum melongena L.) utilizing the cytoplasm of S. virginianum, an interspecific F₁ hybrid between S. virginianum and S. melongena ‘Senryo Nigou’ was continuously backcrossed to S. melongena ‘Uttara’ using ‘Uttara’ as a recurrent pollen parent and four backcross generations, BC₁, BC₂, BC₃ and BC₄ were produced. All the plants in backcross generations were anther indehiscent although the F₁ hybrid, S. virginianum and S. melongena were dehiscent. Pollen stainability with acetic carmine and in vitro germination ability of pollen in all the backcross progenies were quite lower than those of the parental species. Fruit set percentage, number of seeds per fruit and seed germination rate were high in all the backcross progenies. The present results indicate that anther indehiscent type of functional male sterile line of eggplant could be developed by utilizing the cytoplasm of S. virginianum. PCR-RFLP analysis of chloroplast DNA (cpDNA) and mitochondrial DNA (mtDNA) were performed to identify the organelle inheritance. The F₁ hybrid and all the backcross progenies displayed maternal inheritance of mtDNA. The cpDNA of the examined single BC₁ plant exhibited the recombinant cpDNA pattern of the parental F₁ hybrid and ‘Uttara’, indicating occurrence of the biparental inheritance of cpDNA. As all the BC₂, BC₃ and BC₄ progenies showed the same recombinant cpDNA patterns of the BC₁ plant, the recombinant cpDNA might be stable and harmonize with the nuclear genome of S. melongena. The present male sterile line can contribute to expand the male sterility source of eggplant.     

The effect of nitrogen fertilization on plant growth and the nitrate content of leaves and roots of parsley in the Mediterranean region

Curly-leafed and turnip-rooted parsley have recently been introduced to Greece as alternative herbs within the programme for crop diversification. Because the response of these subspecies to nitrogen (N) application under the warm climatic conditions of the Mediterranean region is unknown, both were cultivated over three consecutive years in order to evaluate their yield potential and quality (in terms of nitrate content) in relation to nitrogen application in comparison with the traditionally cultivated plain-leafed subspecies. Nitrogen was applied as ammonium nitrate in the form of liquid feeds at rates of 30–450 mg kg⁻¹. Foliage and root yield increased with N application up to a level of 150 mg kg⁻¹ and the nitrate content of the tissues was low. Increasing N application rates to 300 or 450 mg kg⁻¹ did not cause a further increase in yield, but the nitrate content of the tissues increased. In view of health concerns with respect to human nitrate intake and environmental threats posed by excessive N application, it is concluded that the optimum level of ammonium nitrate application under local conditions should be 150 mg kg⁻¹. Overall, all three subspecies responded to N in a similar way in terms of yield and nitrate content.     

Development of a disease severity rating scale for plane tree anthracnose

Anthracnose caused by the fungus Apiognomonia veneta (Sacc. et Speg.) Höhnel., is the most important and frequent disease affecting mature ornamental plane trees (Platanus×hybrida Brot.=P.×acerifolia (Ait.)Willd.) of central Spain. Symptoms of the disease are: leaf vein and petiole necrosis, bud death, defoliation, proliferation of shoots growing in whorls, short internodes, cankers, necrotic lesions and twigs and branches growing in angles. Based on our previous experience, a disease severity rating scale (DSRS) has been established, consisting of six levels: 0=healthy, 1=initial, 2=low, 3=medium, 4=high and 5=dead. In order to achieve a better and more efficient measure of disease intensity we tried to quantify this visual scale using mathematical criteria. In 2000, 610 mature trees up to 200 years old, divided into 4 age classes, were selected and systematically evaluated using a visual estimation based method. The trees were located in central Spain, mainly in two periurban areas of the town of Aranjuez (south of Madrid) and in three urban areas of Madrid city. Trees were observed in late spring, summer and winter. We recorded visual estimation of disease severity, foliage cover, healthy new shoots, dead branches, shoot growth in whorls and branch growth in angles. The statistical relationship between the parameters, disease severity and all the variables recorded (defoliation, healthy new shoots, etc.) has been evaluated. “Leaf density”, “dead branches” and “healthy shoots” were the variables that help to discriminate better between the different levels of the DSRS. A clearer definition of the different phases of disease severity will facilitate the application of possible control methods and the prediction of the behaviour of other Platanus spp.     

Root induction in three species of bamboo with different rooting abilities

Rooting of in vitro axillary shoots from adult field culms of Bambusa atra, Dendrocalamus giganteus and D. hookeri and of juvenile seedling shoots of D. giganteus were investigated. B. atra rooted spontaneously without exogenous auxin during axillary shoot proliferation, while both Dendrocalamus species rooted only on transfer to rooting media with indole-3-butyric acid (IBA). D. giganteus required coumarin, an auxin protector, in addition to IBA. Rooting in adult shoots of D. giganteus was lower (45.6%) than that in the juvenile shoots (96.7%) but adult shoots of D. hookeri rooted well (88.9%). A pretreatment with thidiazuron (TDZ: N-phenyl-N-[(1,2,3-thidiazol-5-yl)urea]) induced development of axillary buds and subsequently 95% rooting in adult D. giganteus shoots but had no significant effect on rooting in juvenile shoots of D. giganteus or D. hookeri. Pretreatment with tri iodobenzoic acid (TIBA) arrested shoot and bud development leading to very low or no rooting in all three species.     

Identification of host plant resistance to pepper leaf curl virus in chilli (Capsicum species)

Three hundred and seven genotypes belonging to four cultivated and one wild species of Capsicum were screened against pepper leaf curl virus (PepLCV) causing devastating leaf curl disease of chilli (Capsicum annuum). Initial screening was done under field conditions based on coefficient of infection (CI), disease reaction to each genotype was assigned. Subsequently, selfed progenies of eight symptom-less and highly resistant lines were challenged by viruliferous white fly under glasshouse conditions, out of which only three genotypes, viz. GKC-29, BS-35 and EC-497636 showed no symptom. Using scion and root stalk of susceptible genotype (Pusa Jwala), these three putative symptom-less genotypes were further challenged by grafting and alternate grafting. The resistant reactions of GKC-29, BS-35, EC-497636 were confirmed because even after 50 days of successful grafting/alternate grafting, no viral symptom appeared on all the grafted plants of these genotypes. When subjected to PCR amplification with degenerate primers deigned to detect gemnivirus like PepLCV, the three symptom-less genotypes did not show any amplification, suggesting that the resistant reaction in three identified symptom-less resistant sources was because of the absence of viral genome and they are not symptom-less carrier.     

Stable Isotope Signatures and Landscape Functioning in Banded Vegetation in Arid-Central Australia

We examined how the measurement of stable carbon and nitrogen isotopes in soils, vegetation and invertebrates can contribute to understanding landscape processes in mulga Acacia aneura ecosystems characterised by alternating wooded groves and intergroves. Our analyses showed that greater leakiness of water from intergroves at the landscape scale tended to promote more conserving physiology at the plant scale. Thus isolated mulga trees in intergroves probably have higher water use efficiency than those in groves. Both trees and grasses in the intergroves have a greater reliance on recycled nitrogen than plants in the groves for which recently fixed N was a substantial source. Grasses in the intergroves had higher N concentrations than those in the groves despite the soil having lower N concentrations. A lack of variation in the isotopic signature of surface soil N suggested that the lower N concentrations in soils of intergroves than groves is due to lower rates of input and not shorter residence times. Stable isotopic signatures of invertebrates showed a diversity of feeding strategies amongst termite species and indicated symbiotic N fixation in two species. There was no relationship between the dependence on N fixation and the habitat preference or diet of termites. Our results suggest that with cautious interpretation, stable isotope signatures could contribute to understanding other ecosystems where patch-interpatch functioning is an important landscape process.     

Nesting Success of a Songbird in a Complex Floodplain Forest Landscape in llinois, USA: Local Fragmentation vs. Vegetation Structure

Measuring edge effects in complex landscapes is often confounded by the presence of different kinds of natural and anthropogenic edges, each of which may act differently on organisms inhabiting habitat patches. In such landscapes, proportions of different habitats surrounding nests within patches often vary and may affect nesting success independently of distance to edges. We developed methods to measure and study the effects of multiple edges and varying habitat composition around nests on the breeding success of the Acadian flycatcher (Empidonax virescens), an understory, open-cup nesting songbird. The Kaskaskia River in Southwestern Illinois was our study area and consists of wide (>1000-m) floodplain corridors embedded in an agricultural matrix with a variety of natural (wide rivers, backwater swamps, and oxbow lakes) and anthropogenic (internal openings, and agricultural) habitats. We also measured vegetation structure around each nest. Nest survival increased with increasing nest concealment, and probabilities of brood parasitism increased with increasing distances from anthropogenic and natural water-related openings surrounding nests. The magnitude of these effects was small, probably because the landscape is saturated with nest predators and brood parasites. These results illustrate the importance of considering both larger landscape context and details of natural and anthropogenic disturbances when studying the effects of habitat fragmentation on wildlife.     

Effects of thermoperiodicity and plant population density on stem and flower elongation, leaf development, and specific fresh weight in single stemmed rose (Rosa hybrida L.) plants

Thermoperiodicity, i.e. growth in the alternating temperature regime with the same diurnal mean compared with growth at the constant temperature at which optimal growth occurs, was studied at three plant population densities in four cultivars of Rosa hybrida L. Single-node cuttings with five-leaflet leaves were excised and grown as single-stemmed rose plants at an average photosynthetic photon flux density of about 260 μmol m⁻² s⁻¹ and supplied with carbon dioxide at about 1000 μmol mol⁻¹. The optimal constant temperature regime was 22 °C day (20 h)/22 °C night (4 h); alternating temperatures were 23 °C day (20 h)/18 °C night (4 h). The plant population densities were 100, 131 and 178 plants m⁻² of bench area. Thermoperiodicity was absent, or could not be detected, in the parameters related to the growth period, the formation of fresh biomass, the bloom quality, and most parameters related to shoot elongation. However, classic thermoperiodic effects of alternating regime were significant in the cultivars Red Velvet and Sonia, with shoot elongation promoted (7.1 and 10.5%, respectively) in the growth phase from onset of axillary bud growth until the flower bud became visible. Compared with the other two cultivars, plants of Red Velvet and Sonia tended to develop longer internodes. The results, obtained concurrently at three different plant population densities, suggest that thermoperiodicity can affect (single-stemmed) plant growth and development in R. hybrida. Increased plant population density also increased plant height at visible flower bud, but the bloom quality, expressed as specific fresh weight, and the flower height at anthesis was decreased at the highest density. Increased plant population density increased the number of five-leaflet leaves developed in Red Velvet, but had no effect on leaf number in Texas and Sonia, while, in Lambada the leaf number was decreased at the highest density.     

Role of bioinoculants in development of salt-resistant saplings of Morus alba (var. sujanpuri) in vivo

Pot experiments were conducted for the development of salt-resistant saplings of Morus alba (var. sujanpuri) involving bioinoculants, namely arbuscular mycorrhizal (AM) fungi, Azotobacter and indole butyric acid (IBA). The IBA and sodium chloride (NaCl) concentrations were optimized prior to the experiments. By using both low and high concentrations, 15 ppm IBA and 0.05% NaCl (w/v) were found to be optimum in acting synergistically with AM fungi and Azotobacter and also for increasing all the growth parameters and microbial count in the rhizosphere. For in vivo development of salt-resistant saplings, the optimal concentration of IBA, along with AM fungi and Azotobacter in different combinations, was applied. The saplings were irrigated regularly with 0.05% NaCl water. Although growth parameters such as AM infection percentage, AM spores per 100 g soil and Azotobacter cells/g soil were affected by NaCl watering, the inoculation of both bioinoculants significantly enhanced survival percentage of saplings from 25 to 50% under salt stress. Maximum survival (55%) of saplings was found with IBA (15 ppm)+AM fungi+Azotobacter.     

Use of space by the yellow-footed antechinus, Antechinus flavipes, in a fragmented landscape in South Australia

An understanding of how individual species are able to persist and move within fragmented landscapes is critical for elucidating the effects of fragmentation and aiding in the management of species. Here, we studied movement behaviour of the dasyurid Antechinus flavipes in a heavily fragmented landscape using trapping and radiotracking. We assessed the ability of animals to move within and amongst small (<6 ha) remnants and make use of the matrix, and investigated how females used the available space within remnants. Seventeen between-remnant movements were detected from 428 recaptures, ranging in length from 30 to 720 m and averaging 352 m. Most were by adult males during the breeding season, with 40% more than 500 m. Landscape types traversed would have included exotic pine plantations, open grazed areas and roads. Between-site movements of juveniles were only detected on three occasions. However, few young males were captured as adults, suggesting high dispersal rates and considerable matrix use. Conversely, despite high female recapture rates, again only three between-site movements were recorded. Radiotracking further indicated that females confined foraging to remnants, with occasional forays to isolated trees in paddocks. Female home range areas were similar for remnants and forest (0.04–0.66 ha). A. flavipes is clearly able to persist in very small patches of native vegetation in the landscape studied here. Its long-term persistence appears dependent on the ability of females to maintain a presence in the small remnants, and of unrelated males to move between remnants to breed with resident females. This study illustrates the importance of recognising the occurrence of metapopulations in fragmented landscapes for conservation management.     

Effects of fire frequency on plant communities and landscape pattern in the Massif des Aspres (southern France)

Fire frequency can affect pattern and diversity in plant communities and landscapes. We had the opportunity to study changes due to recurring wildfires on the same sites over a period of 50 years in the Massif des Aspres (southern France). The study was carried out in areas occupied byQuercus suber andQ. ilex series. A comparison of historical and cartographical documents (vegetation maps covering a 50 year interval and an accurate map of major wildfires during this period) allowed us to determine the changes occurring over time with or without fire action. Plant communities were grouped into three main vegetation types: forests, treed shrublands and shrublands. The passage of three successive wildfires on the same site led to a decrease in forest areas and an increase in shrublands; however, shrublands were already present before the first fire of the period under consideration. Less frequent fire occurrence induced more complex heterogeneity and greater landscape diversity. In the study region as a whole, with or without fire action, a significant decrease in forest surfaces was recorded, whereas there was an increase of unforested communities such as treed shrublands and shrublands. In some parts of the Massif fires increased the homogeneity of the landscape, in other parts they created a greater heterogeneity and diversity of plant communities.     

In vitro induction of the amphiploid in interspecific hybrid of blueberry (Vaccinium corymbosum × Vaccinium ashei) with colchicine treatment

Amphiploids were produced from pentaploid hybrids between Vaccinium corymbosum (4x) and Vaccinium ashei (6x) by colchicine treatment. Seeds of each parental species and those obtained by the interspecific crossing were treated with colchicine at 0, 500, 1000 and 2000 mg/l for 7 days, and the ploidy level of the seedlings was determined with flow cytometry. Either amphiploid (10x) or ploidy chimera (5x + 10x) was obtained from interspecific hybrid seeds treated with all colchicine concentrations, while no chromosome-doubled plants were obtained from both parents. Cross direction in interspecific hybridization affected the results of colchicine treatment and amphiploids were obtained only when V. corymbosum was used as the seed parent. In this cross, 17–25% of the seedlings turned to be amphidiploids or ploidy chimeras by treating with 500 mg/l colchicines for 7 days. These results indicate that susceptibility to colchicine may increase in the interspecific hybrid compared with the parental species but only when V. corymbosum was used as female parent.     

Distribution of three indigenous fern species along a rural–urban gradient in the city of Angers, France

The preservation of wild plants and animals in urban environments can be a good means to meet the demand for natural areas for recreational purposes. However, the impacts of urbanisation on native species distribution are poorly studied. A city environment has high impact on vegetal community dynamics, especially in terms of climate modification, level of perturbationand pattern of dispersion. We chose to study the three indigenous species of the genus Polypodium that are known to grow in a wide range of habitats, including forest and urban environments, and exhibit a priori a strong ability for dispersal. The aim of the study was to evaluate the factors involved in the Polypodium species distribution and to determine whether this distribution was influenced by the rural–urban gradient. The distribution of the three fern species was investigated in the city of Angers (France) at two levels: for a park (urban woodland) and for the whole city. A contrasted distribution of the three Polypodies has been recorded and shows a rural–urban gradient. This can be explained by the ecology of each species and some biological traits. It means that differences in the spatial structure of the city lead to particular patterns of distribution for these plants. Thus, preserved indigenous vegetation may be influenced in its species composition by the surrounding urban development.     

Estimation and prediction of plant species richness in a mosaic landscape

Traditional agricultural mosaic landscapes are likely to undergo dramatic changes through either intensification or abandonment of land use. Both developmental trends may negatively affect the vascular plant species richness of such landscapes. Therefore, sustainable land-use systems need to be developed to maintain and re-establish species richness at various spatial scales. To evaluate the sustainability of specific land-use systems, we need approaches for the effective assessment of the present species richness and models that can predict the effects on species richness as realistically as possible. In this context, we present a methodology to estimate and predict vascular plant species richness at the local and the regional scale. In our approach, the major determinants of vascular plant species richness within the study area are taken into consideration: These are according to Duelli's mosaic concept the number of habitat types and of habitat patches within area units. Furthermore, it is based on the relative frequencies of species within habitat types. Our approach comprises six steps: (i) the determination of present habitat patterns within an observation area, (ii) the creation of a land-use scenario with simulated habitat patterns, (iii) the determination of species frequencies within habitat types of this area, (iv) a grouping of habitat-specific species, (v) the estimation of the probabilities for all species (or habitat specialists) to occur, either in stepwise, exponentially enlarged landscape tracts (local scale), or in the entire observation area (regional scale), and (vi) the validation of the estimated species numbers. The approach will be exemplified using data from the municipal district of Erda, Lahn-Dill Highlands, Germany. The current species numbers to be expected on the basis of probability calculations were compared with those recorded on the basis of extensive field work. This comparison shows that, on the basis of our simple calculations, the current local plant species richness can be predicted well, with a slight underestimation. This revised version was published online in May 2005 with corrections to the Cover Date. This revised version was published online in July 2006 with corrections to the Cover Date.