应用电子显微镜技术对CAA和IBDV混合感染的研究

应用电子显微镜技术在鸡传染性法氏囊病(IBD)的自然病例中见到了大量的IBDV和一种较小的病毒粒子。这种小病毒粒子直径大约19nm,球形,无囊膜。免疫电镜的结果表明这种小病毒粒子是鸡贫血因子(CAA)。实验感染的SPF鸡表现出了与自然病例相似的临床症状、病理变化以及高死亡率,并且又重新分离到CAA和IBDV。除了这两种病毒之外,没有发现其它病毒。笔者认为混合感染,特别是与CAA的双重感染是高死亡率的IBD的重要原因。     

麻雀自然感染鸡传染性法氏囊病病毒的调查

从鸡传染性法氏囊病（IBD）流行的鸡场捕杀麻雀54只,用鸡传染性法氏囊病病毒（IBDV）单克隆抗体夹心阻断ELISA检测抗体,阳性检出率为7.4％(4/54);以逆转录—聚合酶链反应（RT-PCR）检测病毒核酸,阳性检出率为11.1％（6/54）;RT-PCR阳性样本病毒分离亦为阳性。结果表明,IBD流行的鸡场里的麻雀能够发生IBDV自然感染,麻雀可能是IBDV的贮存宿主或二次传染源之一。     

美国鹧鸪人工感染鸡传染性法氏囊病的试验

鸡传染性法氏囊病(IBD)是一种主要侵害雏鸡的免疫抑制性、高度接触性疾病,该病原为鸡传染性法氏囊病病毒(IBDV),主要侵害的靶器官是法氏囊。美国鹧鸪(石鸡)在解剖和生理上与鸡相似,在生产实践中,能有效治疗鸡法氏囊病的高免卵黄抗体,也对临床上出现类似IBD症状的美国鹧鸪有疗效,为了进一步确定美国鹧鸪对IBDV的易感性而进行本试验。     

传染性法氏囊病病料中MDV、CAV、REV的共感染检测

从江苏、山东、浙江、河南、上海、广西、云南等地收集根据临床表现及病理变化诊断为传染性法氏囊病（IBD）的病鸡法氏囊样品66份，用相应的核酸探针及斑点杂交法分别检测样品中鸡传染性贫血病病毒（CAV）、马立克氏病病毒（MDV）、网状内皮细胞增生病病毒（REV）的感染；用抗IBD血清做琼扩检测IBDV。结果显示，部分地区病料中这些病毒有不同程度的二重感染、三重感染甚至四重感染。提示在研究特定毒株IBDV的毒力时，应考虑多重感染对致病性的影响。     

鸡传染性法氏囊病病毒不同攻毒方法致病效果试验

为建立传染性法氏囊病病毒(IBDV)对雏鸡的最佳感染模型,采用点眼滴鼻、肌肉注射、擦肛和泄殖腔内灌注等不同方法,对SPF鸡和普通鸡进行了攻毒致病试验。结果表明,以泄殖腔内灌注方法攻毒,可使雏鸡发生传染性法氏囊病(IBD)速度快,病死率高,临床症状和病理变化典型。该试验结果为制备鸡传染性法氏囊病感染模型,提供了精确、简单的技术支持。     

鸡传染性法氏囊病与鸡新城疫相关性研究简述

鸡传染性法氏囊病(IBD)是威胁我国养鸡业的鸡的几大传染病之一,目前我国许多省市都已有IBD的发生及流行,并造成了巨大的经济损失。由于鸡传染性法氏囊病病毒(IBDV)侵害感染幼鸡的体液免疫中枢器官—法氏囊,使病鸡不能正常产生免疫细胞,而造成病鸡的免疫抑制。IBD的发生使病鸡对其他疾病的易感性增高,并常造成其他疫苗的免疫失败。临床常见由于IBD的发生而继发新城疫     

传染性法氏囊病病毒变异的分子基础

鸡传染性法氏囊病病毒(IBDV)是传染性法氏囊病(IBD)的病原体。由于急性感染的高死亡率和亚临床感染严重的免疫抑制,IBDV对养禽业经济上有重大的影响[11,14]。IBDV共有两个血清型,即1型和2型,血清1型对鸡有致病性,血清2型分离自火鸡,对鸡无致病性。J.Rosenberger(1985)首次从美国特拉华半岛肉鸡群分离到4株IBDV变异株,1987年,荷兰、比利时爆发了与美国株不同的超强病毒(vvIBDV)。而后,英国、法国、西班牙、德国等欧洲国家相继分离到超强毒株。李树根(1991)等首次在国内分离到血清亚型株,李德山(1991)首次报道了中国超强毒株,朱爱国…     

囊炎康防治鸡传染性法氏囊病效果好

鸡传染性法氏囊病(IBD)发生面广,发病率高,并容易并发和继发其他传染病,对养禽业的发展造成极大威胁。对鸡传染性法氏囊病以往我们主要采用多种西药治疗,效果都不十分理想,采用IBD高免蛋黄液和高免血清治疗,又存在隐性垂直感染因子危害的弊端。从1998年开始我们应用纯中药制剂——囊炎康防治鸡传染性法氏囊病,收到较好的防治效果。现将有关情况总结如下,供同行参考。(一)临床症状鸡传染性法氏囊病(IBD)也称传染性囊病或腔上囊炎,是由传染性法氏囊病毒(IBDV)引起的一种幼鸡(青年鸡)急性接触性免疫抑制性传染病。该病主要特性是因免疫失…     

鸡传染性法氏囊病防治的措施

鸡传染性法氏囊病（IBD）是由传染性法氏囊病毒（IBDV）引起的。致病特性以亚临床型IBD症状为主,最典型的病变是法氏囊迅速萎缩,未见经典毒株所致的高死亡率及典型的腿肌出血等症状。当常规IBD疫苗免疫的鸡群受到变异毒株侵袭时,     

白羽肉鸡传染性法氏囊疾病防控

<正>鸡传染性法氏囊病(infectious Bursal Disease,IBD)是由呼肠弧病毒引起的一种急形、高度传染性疾病。由于该病发病突然、病程短、死亡率高,且可引起鸡体免疫抑制,目前仍然是白羽肉鸡主要传染病之一。1鸡传染性法氏囊病的病原学及临床症状IBDV有两个血清型,鸡源性病毒为血清I型,能引起鸡发病;血清II型毒株来源于火鸡,对鸡和火鸡都无致病力。IBD出现的初期是以经典毒株     

鸡Toll样受体在IBDV免疫中的作用研究

为了揭示鸡先天性免疫反应在IBDV疫苗过程中的作用,将40只14日海兰褐蛋鸡随机分成两组,实验组接种IBDV活疫苗,对照组注射生理盐水,隔离饲养。于免疫前和免疫后12h、24h、48h、72h、130h各取脾脏和法氏囊分离淋巴细胞并提取总RNA,用实时荧光定量检测方法检测各组中chTLR3、chTLR7、chTLR21表达的动态变化。结果显示,在免疫后36h,鸡法氏囊和脾脏中chTLR3表达变化最显著,在免疫后48h,鸡脾脏和法氏囊中chTLR21表达变化也较为明显。提示chTLR3在鸡对IBDV的免疫应答中起主要作用。     

鸡传染性法氏囊病病理组织学图谱

传染性法氏囊病(Infections Bursal Disease)又称传染性法氏囊炎,是由传染性法氏囊病病毒引起的一种破坏鸡免疫中枢器官--法氏囊的急性接触性传染病.自1957年起,先后发生于美国、法国、加拿大、日本等20多个国家和地区.     

Susceptibility of ducks and duck-origin cell cultures to infectious bursal disease virus

Specific-pathogen-free (SPF) ducks that were 1, 3, 4, 7, 10, 30, and 180 days old were inoculated experimentally orally or nasally with infectious bursal disease virus (IBDV). Attempts to induce clinical disease in ducks with strain J1 or FK-78 of IBDV were unsuccessful. Virus-recovery attempts from organ and intestinal contents were also unsuccessful. No significant gross or histopathological lesions were found in liver, spleen, kidney, heart, or bursa of Fabricius of 1- and 3-day-old ducks at 4 or 7 days postinoculation. The ratios of bursa weight to body weight of 1-, 10-, and 30-day-old inoculated and control ducks revealed no difference at 21 days postinoculation. The ducks responded serologically, however, by developing both virus-neutralizing and agar-gel-precipitin antibodies. Virus multiplied in embryonated duck eggs and duck embryo fibroblast cells but not in duck kidney cells.     

Morphologic changes in the bursa of fabricius of chickens after inoculation with infectious bursal disease virus.

Sequential morphologic changes in the bursa of Fabricius were studied after oral inoculation of 1-day-old chicks with infectious bursal disease virus (IBDV). The epithelial surface morphology was studied by scanning electron microscopy, whereas the IBDV replication was sequentially followed by immunofluorescence and transmission electron microscopy. The earliest detectable changes in the bursal epithelium were evident at postinoculation hour (PIH) 48. They were characterized by reduction in numbers and size of microvilli on the epithelial cells accompanied by gradual involution of the button-like bursal follicles. At PIH 96 some specimens showed localized surface erosions due to loss of epithelial cells. As the damage progressed, the infolding of the buttomlike follicles became more pronounced and the surface erosions became more extensive. Loss of surface epithelium exposed the underlying damaged bursal follicles which appeared to be bounded by columnar epithelium. Some follicles had lost almost all the lymphocytes and macrophages and appeared as empty craters. Intrafollicular replication of IBDV was detectable as early as PIH 24 by immunofluorescence technique. Viral replication primarily took place in the lymphoid follicles. Regeneration of the follicles was not seen up to postinoculation day 12, suggesting that the IBDV-induced bursal damage could be permanent.     

Pathogenicity of an isolate of infectious bursal disease virus in local Nigerian ducks

Summary Three to six-week old local Nigerian ducks were inoculated orally and intraocularly with an isolate of infectious bursal disease virus (IBDV) serotype 1 which was pathogenic for chickens. Neither clinical signs nor gross or microscopic lesions were observed. IBDV antigen was not detected in the bursa by the agar gel diffusion precipitation test. Precipitins and neutralising antibodies to IBDV were also not detected in the serum samples and IBDV was not isolated from the bursa, spleen and liver of the ducks. The above observations suggest that the ducks might be resistant to IBDV infection.

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Patogenicidad De Una Cepa Del Virus De La Enfermedad Infecciosa De La Bolsa En Patos Nigerianos Locales

Resumen Se inocularon patos nigerianos locales de tres a cuatro semanas de edad, vía oral, con una cepa del virus de la enfermedad infecciosa de la bolsa (EIVB), serotipo I, el cual fue patógeno en pollos. No se observaron sintomas clínicos ni lesiones microscópicas. No se detectó antígeno de EIVB en la bolsa, mediante la prueba de difusión precipitación en agar gelatina. No se detectaron anticuerpos neutralizantes, ni precipitinas de EIVB en las muestras de suero. EI virus no se aisló tampoco de la bolsa, bazo e hígado de los patos infectados. Estas observaciones sugieren que los patos pudieran ser resistentes a la EIVB.

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Pathogenicite D'un Isolat De Bursite Infectieuse Sur Des Canards Nigerians De Souche

Résumé Des canards nigérians de 3 à 6 semaines ont reçu, par injection orale et intraoculaire, un isolat du virus de la Bursite infectieuse (sérotype I), pathogène pour les poulets. Ni signes cliniques ni lésions macro ou microscopiques n'ont été observées. L'antigène viral de la Bursite infectieuse n'a pas été détecté dans les séreuses par le test de précipito—diffusion en agar. De même les précipitines et les anticorps neutralisants n'ont pas été isolés des séreuses, du foie et de la rate des canards. Ces observations montrent que les canards pourraient être résistants au virus de la Bursite infectieuse.

     

Electron microscopy of bursa of Fabricius of chicks infected with a field strain of infectious bursal disease virus

Chicks were infected in the bursa with a field strain of infectious bursal disease virus. Inter- and intracellular edema, condensation and margination of nuclear chromatin, increased number of lysosomes in macrophages, and lymphocytolytic changes appeared earliest by 8 hours post infection. Inclusions containing spheroid to hexagonal virus particles were seen in the cytoplasm of the macrophages. Multiplying virus particles in crystalline arrays arranged either in single or in multiple clusters were seen in the cytoplasm of macrophages, lymphocytes and light stained reticular epithelial cells.     

Immunohistological identification of both infectious bursal and Marek virus antigens in the bursa of Fabricius

Indirect Immunoperoxidase (IIP) and Avidin Biotin-Peroxidase Complex (ABC) techniques were used for the detection of Infectious Bursal Virus (IBV) and Marek Disease Virus (MDV) antigens in alcohol and formalin-fixed, paraffin-embedded lymphoid tissues from broilers. Both techniques appeared potentially useful for the diagnosis of both viral antigens in alcohol-fixed tissues, and allowed the observation of dual infection in the bursa of Fabricius of the studied animals in a natural infection.