Yield potential and water use efficiency of aerobic rice (Oryza sativa L.) in Japan

Intensive rice farming in aerobic soil, referred to herein as aerobic rice, can greatly reduce the water input compared to that of flooded rice cultivation. The objective of this study was to compare the potential productivity of aerobic rice and flooded rice using high-yielding varieties at two locations in Japan in two successive years. In aerobic fields, the total amount of water supplied (irrigation plus rainfall) was 800–1300 mm. The soil water potential at 20-cm depth averaged between −15 and −30 kPa each growing season, but frequently reached −60 kPa. The average yield under aerobic conditions was similar to or even higher than that achieved with flooded conditions (7.9 t ha⁻¹ in 2007 and 9.4 t ha⁻¹ in 2008 for aerobic versus 8.2 t ha⁻¹ for flooded). The average water productivity under aerobic conditions was 0.8–1.0 kg grain m⁻³ water, slightly higher than common values in the literature. The super-high-yielding cultivar Takanari achieved yields greater than 10 t ha⁻¹ with no yield penalty under aerobic conditions in 3 out of 4 experiments. The favorable agronomic characteristic of Takanari was its ample sink capacity (grain number × grain weight). In conclusion, high-productivity rice cultivation in aerobic soil is a promising technology for water conservation. With continued breeding, future aerobic rice varieties will possess large numbers of spikelets and sufficient adaptation to aerobic conditions such that they will consistently achieve yields comparable to the potential yield of flooded rice.     

Management of complex weed flora in dry-seeded rice

Dry-seeded rice has been introduced as an alternative to puddled hand-transplanted rice in the north Indian states of Punjab and Haryana. In dry-seeded rice, weed flora tends to be more diverse and weeds emerge in several flushes during the crop growth cycle and substantial yield reductions due to weed competition are quite common. The efficacy and compatibility of tank mixtures of different herbicides for the control of diverse weed flora in dry-seeded rice was evaluated in field experiments during the summer seasons of 2012 and 2013. The tank mixture of fenoxaprop with ethoxysulfuron improved the control of Echinochloa crus-galli and Echinochloa colona by 43–69% as compared to fenoxaprop alone while the tank-mix of azimsulfuron with fenoxaprop was antagonistic and reduced the control of Leptochloa chinensis by 86% as compared to fenoxaprop alone. Addition of azimsulfuron or ethoxysulfuron to bispyribac did not improve the control of grass weeds as compared to bispyribac alone. Weed control with the mixture of bispyribac and fenoxaprop varied over the two years. In 2012, bispyribac and fenoxaprop mixture was antagonistic for the control of Dactyloctenum aegyptium, Acrachne racemose, and L. chinensis but in 2013, there was no apparent antagonism and the addition of bispyribac to fenoxaprop reduced grass weed biomass as compared to fenoxaprop alone. In 2013, there was a strong negative correlation (r = −0.95, P < 0.001) between weed dry matter at 45 days after sowing and rice grain yield. According to the linear regression, rice crop is likely to produce no grain yield when weed dry matter exceeds 400 g m⁻². Over the two seasons, fenoxaprop-ethoxysulfuron tank-mix produced similar grain yields (5.6–6.2 t ha⁻¹) to the weed-free check (5.6–7.1 t ha⁻¹). At the farmer fields, rice grain yield in the plots treated with pendimethalin followed by post-emergence bispyribac or a tank-mix of fenoxaprop + ethoxysulfuron ranged from 6.2 to 7.7 t ha⁻¹ as compared to 5.3–5.6 t ha⁻¹ in the plots treated with pendimethalin alone. The tank mixture of fenoxaprop with bispyribac needs further evaluation as this mixture has the potential to effectively control aerobic and aquatic grasses in dry-seeded rice. Single hand weeding prevented crop yield loss from weeds that escaped herbicide treatments only when it was performed within six weeks of sowing.     

Yield and agronomic performance of maize hybrids resistant to the maize weevil Sitophilus zeamais Motschulsky (Coleoptera: Curculionidae)

This study evaluated improved maize hybrids (Zea mays L.) with varying level of resistance to the maize weevil, Sitophilus zeamais Motschulsky, for yield and agronomic traits for two seasons. A total of 22 improved maize hybrids and three commercial standards were tested. Out of the 22 tested, two hybrids CKPH08035 (7.4–9.9 t ha⁻¹) and CKPH08039 (7.3–9.8 t ha⁻¹) significantly out yielded the commercial standards WH505 (8.8 t ha⁻¹) and BH140 (5.5 t ha⁻¹). In addition to yield, the improved hybrids also possess desirable traits including good plant height, good plant and ear aspects and good husk cover. The hybrid CKPH08004 had the lowest Dobie index of susceptibility and was regarded as resistant to S. zeamais. Weevils fed with the resistant hybrids produced low numbers of F₁ generation weevils, had a high median developmental time and a low percentage of grain damage and grain weight loss. An increasing number of F₁ generation resulted in an increasing grain damage and grain weight loss. We found an inverse relationship between the susceptibility index and percent mortality. However, the numbers of F₁ generation, percent grain damage and grain weight loss were positively correlated with the susceptibility index. The use of resistant maize hybrids should be promoted in managing S. zeamais in stored maize under subsistence farming conditions in Africa.     

Wheat grain tissue proportions in milling fractions using biochemical marker measurements: Application to different wheat cultivars

Measurement of biochemical markers allows the quantification of wheat (Triticum spp.) grain tissue proportions in milling fractions. In order to evaluate the ability of extending this methodology to an unknown wheat grain batch, the variability of the markers in the different tissues was assessed on various wheat cultivars. Ferulic acid trimer amounts in the outer pericarp ranged from 0.97 to 1.67 μg mg⁻¹ (dm) with an average value equal to 1.31 μg mg⁻¹ (dm). Alkylresorcinols amounts in a composite layer, including the testa, the inner pericarp and the nucellar epidermis, ranged from 10.5 to 16.7 mg g⁻¹ (dm), with an average value equal to 14.0 mg g⁻¹ (dm). In the aleurone layer, phytic acid amounts ranged from 94.9 to 187.2 mg g⁻¹ (dm) with an average value equal to 152 mg g⁻¹ (dm) whereas, para-coumaric acid ranged from 0.08 to 0.29 μg mg⁻¹ with an average level of 0.18 μg mg⁻¹. In the embryonic axis, wheat germ agglutinin ranged from 879 μg g⁻¹ to 2086 μg g⁻¹ with an average value of 1487 μg g⁻¹. The impact of this variability on tissue proportion determination was evaluated and a strategy to decrease the prediction error was suggested. Percentages of the outer pericarp, intermediate layer (including the testa), aleurone layer and embryonic axis within grains were calculated and their variability discussed.     

Direct-seeded rice culture in Sri Lanka: Lessons from farmers

About 95% of the rice grown in Sri Lanka is direct-seeded (wet- and dry-seeding). The average rough rice yield in irrigated predominantly direct-seeded dry zone (DZ) is about 5.0 t ha⁻¹and in the wet zone (WZ) it is about 3.3 t ha⁻¹. However the average realizable yield in DZ and WZ are 8 t ha⁻¹ and 5 t ha⁻¹ respectively. A survey was conducted to understand the cultural practices, farmers’ perceptions and the reasons for the yield gap in direct-seeded rice culture in Sri Lanka. Farmers’ seed rate ranged from 87 to 220 kg ha⁻¹ for intermediate bold-type varieties and from 71 to 176 kg ha⁻¹ for varieties with short round grains. About 90% of the farmers in the DZ and the intermediate zone (IZ) consider both yield potential and duration as criteria in selecting a variety. Among the farmers surveyed, only 21% of the farmers in the DZ, 13% of the farmers in the IZ, and 29% of the farmers in the WZ adhered to the recommended method of basal fertilizer application. Farmers did not adhere to the correct timing of fertilizer application. More than 50% of the cost for rice farming goes to labor, followed by cost of inputs in all climatic zones. Farmers reported that the most important production constraint for direct-seeded rice in the DZ and IZ is the non availability of reliable labor followed by soil problems and weeds. While in the WZ, it is the soil problems specially iron toxicity followed by lower soil fertility. The survey revealed that smaller land holding size, non adherence to the optimum time of farm activity initiation, less efficient use of rain water, higher seed rate and higher cost of production are a few reasons for the existing yield gap. Location-specific technologies for different agro-ecological zones of Sri Lanka should be developed to reduce the cost of production and to increase resource-use efficiency and should be transferred to the farmers to achieve sustainable optimum direct-seeded rice yields.     

Yield and grain uniformity in contrasting rice genotypes suitable for different growth environments

In this study, the effect of crop season on yield and grain weight uniformity was examined in field-grown rice cultivar Tainung 67 and its sodium azide-induced mutant SA419 in 2000 and 2001. In spring, Tainung 67 had greater yield (7.2 mg ha⁻¹) than SA419 (6.2 mg ha⁻¹). Marked yield decline (averaged 27% decline) was found in Tainung 67 when it was grown in autumn. The yield decline resulting from season change was only 5.9% for SA419. The greater yield of SA419 than Tainung 67 in autumn was due to its higher net assimilation rate and better dry matter partitioning during grain filling. The distribution patterns of grain weight differed between the tested genotypes, with greater grain weight variations for Tainung 67 than SA419. Significant panicle branch effects on the distribution pattern of grain weight were also found between Tainung 67 and SA419 with greater variation for the former than the latter. SA419 has several agronomic traits, such as heavier 1000-grain weight and more uniform grain development within a panicle, that makes it a genotype with superior grain quality than Tainung 67.     

Effect of rice establishment methods on weedy rice (Oryza sativa L.) infestation and grain yield of cultivated rice (O. sativa L.) in Sri Lanka

Weedy rice is a great threat to rice production in Sri Lanka. Selective herbicides to manage weedy rice in conventional rice cultivars are not available in Sri Lanka. In the absence of appropriate chemical control measures, cultural approaches may help to achieve effective control of weedy rice. A study was conducted in two consecutive seasons in farmers' fields at three sites (Atalla, Samanthurai, and Girithale villages) in Sri Lanka to evaluate the effect of different establishment methods (farmers' practice, random broadcast, row seeding, seedling broadcast, and transplanted rice) on weedy rice infestation and rice yield. The farmers' practice had a higher number of weedy rice panicles (60–80 m⁻²) than the random broadcast (39–48 panicles m⁻²), seedling broadcast (3–15 panicles m⁻²), and transplanted rice (1.3–3.0 panicles m⁻²) methods. The use of clean rice seeds in the random broadcast method reduced weedy rice seed production by 29–41% compared with the farmers' practice (0.6–2.0 t ha⁻¹). Compared with the farmers' practice, the seedling broadcast method reduced weedy rice seed production by 71–87% and transplanted rice by 95–98%; and increased rice yield by 27–49% (7.5–9.1 t ha⁻¹). At all three sites, the farmers' practice resulted in the lowest grain yield (5.1–6.7 t ha⁻¹). Compared with the farmers' practice, the random broadcast and row seeding methods increased rice yield by up to 21% and 31%, respectively. The findings suggest that the use of clean rice seeds, the use of a row-seeded crop, and the adoption of different rice planting methods may help to suppress the spread of weedy rice.     

Indigenous legume fallows (indifallows) as an alternative soil fertility resource in smallholder maize cropping systems

Widening the range of organic nutrient resources, especially N sources, is a major challenge for improving crop productivity of smallholder farms in southern Africa. A study was conducted over three seasons to evaluate different species of indigenous legumes for their biomass productivity, N₂-fixation and residual effects on subsequent maize crops on nutrient-depleted fields belonging to smallholder farmers under contrasting rainfall zones in Zimbabwe. Under high rainfall (>800 mm yr⁻¹), 1-year indigenous legume fallows (indifallows), comprising mostly species of the genera Crotalaria, Indigofera and Tephrosia, yielded 8.6 t ha⁻¹ of biomass within 6 months, out-performing sunnhemp (Crotalaria juncea L.) green manure and grass (natural) fallows by 41% and 74%, respectively. A similar trend was observed under medium (650–750 mm yr⁻¹) rainfall in Chinyika, where the indifallow attained a biomass yield of 6.6 t ha⁻¹ compared with 2.2 t ha⁻¹ for natural fallows. Cumulatively, over two growing seasons, the indifallow treatment under high rainfall at Domboshawa produced biomass as high as 28 t ha⁻¹ compared with ∼7 t ha⁻¹ under natural fallow. The mean total N₂ fixed under indifallows ranged from 125 kg ha⁻¹ under soils exhibiting severe nutrient depletion in Chikwaka, to 205 kg ha⁻¹ at Domboshawa. Indifallow biomass accumulated up to 210 kg N ha⁻¹, eleven-fold higher than the N contained in corresponding natural fallow biomass at time of incorporation. Application of P to indifallows significantly increased both biomass productivity and N₂-fixation, translating into positive yield responses by subsequent maize. Differences in maize biomass productivity between indifallow and natural fallow treatments were already apparent at 2 weeks after maize emergence, with the former yielding significantly (P < 0.05) more maize biomass than the latter. The first maize crop following termination of 1-year indifallows yielded grain averaging 2.3 t ha⁻¹, significantly out-yielding 1-year natural fallows by >1 t ha⁻¹. In the second season, maize yields were consistently better under indifallows compared with natural fallows in terms of both grain and total biomass. The first maize crop following 2-year indifallows yielded ∼3 t ha⁻¹ of grain, significantly higher than the second maize crop after 1-year indifallows and natural fallows. The study demonstrated that indigenous legumes can generate N-rich biomass in sufficient quantities to make a significant influence on maize productivity for more than a single season. Maize yield gains under indifallow systems on low fertility sandy soils exceeded the yields attained with either mineral fertilizer alone or traditional green manure crop of sunnhemp.     

Implications of genotype × input interactions in breeding superior genotypes for favorable and unfavorable rainfed upland environments

Resource-poor farmers in India cultivate upland rice as a subsistence crop in poor soil with minimum inputs, often applying little or no fertilizer and controlling weeds by hand. Consequently, upland rice yields are very low. In our study, the response to management intensification of fertilizer application at rates of 40 N ha⁻¹, 13 P ha⁻¹, and 16.7 K ha⁻¹ and two weed control treatments as compared with no fertilizer, and one hand weeding practice commonly followed by farmers in rainfed upland areas was examined with a large set of advanced breeding lines and adapted upland varieties tested over 3 years in multi-location trials. Highly significant genotype × environment interaction was observed in combined analyses across environments, leading to sub-grouping of sites into the high-yielding or favorable and low-yielding or unfavorable upland environment groups. A significant effect of management regime was observed. Averaged over 15 environments, the moderate-input treatment out-yielded the low-input treatment by nearly 65% or 0.8 t ha⁻¹ under favorable environments and by nearly 48% (0.3 t ha⁻¹) in unfavorable environments. A significant genotype effect and genotype × input management interaction for yield at favorable sites was observed. However, the same was not significant at unfavorable sites. Varietal differences were relatively small at unfavorable sites across input levels. The heritability estimates for grain yield were moderately high in both moderate- and low-input conditions in favorable environments. The genetic correlation between yields in moderate- and low-input conditions was high in both favorable and unfavorable environments. The study indicated that improved varieties performed well relative to landraces under low-input management. Improved varieties along with modestly intensified management offer an attractive option to increase the productivity of rainfed upland environments. For both favorable and unfavorable environments, indirect selection under moderate-input conditions was less efficient than direct selection for grain yield in low-input conditions, indicating upland breeding programs to adopt selection for grain yield under both moderate- and low-input conditions.     

The implications of land preparation, crop establishment method and weed management on rice yield variation in the rice-wheat system in the Indo-Gangetic plains

The implications of adopting alternative seeding methods for rice and wheat establishment were examined at three geographically separate sites in the rice-wheat system of the Indo-Gangetic plains, across northern India. Rice yields in cultivated plots, established by either wet or dry seeding methods, were evaluated in comparison to yields from zero-tillage plots and under conventional transplanting methods. In the same trials, the effects of crop establishment methods in wheat were assessed both on wheat yields and rice yields. Rice crop establishment methods markedly influenced the emerging weed flora and attainable yields were measured in relation to intensity of weed management. Over four years, average rice grain yields in the absence of weed competition were greatest (6.56 t ha⁻¹) under wet seeding (sowing pre-germinated rice seed on puddled soil), and similar to those from transplanted rice (6.17 t ha⁻¹) into puddled soil, and dry seeded rice after dry soil tillage (6.15 t ha⁻¹). Lowest yields were observed from dry seeded rice sown without tillage (5.44 t ha⁻¹). Rice yield losses due to uncontrolled weed growth were least in transplanted rice (12%) but otherwise large (c. 85%) where rice had been sown to dry cultivated fields or to puddled soil, rising to 98% in dry seeded rice sown without soil tillage. Weed competition reduced multiple rice yield components, and weed biomass in wet seeded rice was six-fold greater that in rice transplanted into puddled soil and twice as much again in dry seeded rice sown either after dry tillage or without tillage. Wheat grain yields were significantly higher from crops sown into tilled soil (3.89 t ha⁻¹) than those sown without tillage (3.51 t ha⁻¹), and also were elevated (5% on average) where the soil had been dry cultivated in preparation for the previous rice crops rather than puddled. The method of wheat cultivation did not influence rice yield. Soil infiltration rates in the wheat season were least where the land had been puddled for rice (1.52 mm h⁻¹), and greater where the soil had been dry-tilled (2.63 mm h⁻¹) and greatest after zero-tillage (3.54 mm h⁻¹).These studies demonstrated at research managed sites across a wide geographic area, and on farmers’ fields, that yields of dry seeded rice sown after dry cultivation of soil were broadly comparable with those of transplanted rice, providing weed competition was absent. These results support the proposition that direct seeding of rice could provide an alternative to the conventional practice of transplanting, and help address rising costs and threats to sustainability in the rice-wheat rotation. Further, analysis of patterns of long-term rainfall data indicated that farmers reliant on monsoon rainfall could prepare fields for dry direct seeded rice some 30 days before they could prepare fields for either transplanting or seeding with pre-germinated seed. Dry, direct seeding of rice contributes a valuable component of an adaptive strategy to address monsoonal variability that also may advance the time of wheat establishment and yield. Whilst the results illustrate the robustness, feasibility and significant potential of direct seeded rice, they also highlight the critical nature of effective weed control in successful implementation of direct seeding systems for rice.     

Enhancing the performance of transplanted coarse rice by seed priming

Raising the rice seedlings in the nursery and its transplantation into the flooded fields is principal method of rice cultivation in the world. Traditional nursery raising method in Pakistan is tedious and produces week seedlings, which reduces the final yield due to high mortality. The potential of seed priming to improve the nursery seedlings and thus the transplanted rice was evaluated in the present study. Seed priming tools employed during the investigation included traditional soaking (pre-germination), hydropriming for 48 h, osmohardening (KCl or CaCl₂) (ψs-1.25 MPa) for 24 h (1 cycle), ascorbate priming (with 10 ppm ascorbate) for 48 h or seed hardening for 24 h. Priming improved nursery seedling vigor and resulted in improved growth, yield and quality of transplanted rice. Osmohardening (KCl) consequently resulted in the best performance, followed by osmohardening (CaCl₂)_, hardening and ascorbate priming. Osmohardening (KCl) produced 4.28 t ha⁻¹ (vs. 3.51 t ha⁻¹ from untreated control) kernel yield, 10.27 t ha⁻¹ (vs. 9.34 t ha⁻¹ from untreated control) straw yield and 29.41% (vs. 27.31% from untreated control) harvest index. The improved yield was attributed to increase in number of fertile tillers.     

Control of chickpea blight disease caused by Didymella rabiei by mixing resistance inducer and contact fungicide

Elicitors of systemic acquired resistance are well known to reduce severity of several plant pathogenic diseases caused by fungi, bacteria and viruses. Their field applications for management of plant diseases are, however, limited because of yield penalties. Our studies on affect of Benzo (1,2,3)-thiadiazole-7-carbothioic acid S-methyl ester (BTH), an elicitor of systemic acquired resistance, on chickpea blight caused by a fungal pathogen Didymella rabiei showed that multiple foliar applications of the chemical were effective in management of the disease under economic threshold levels. Multiple applications, however, affected chickpea grain yield adversely. The BTH induced yield penalties could be prevented by foliar spray schedule comprised of BTH and a contact fungicide mancozeb. One spray of BTH (50 ppm) followed by another of mancozeb (0.2%) was less effective (8.3% severity) than three sprays of BTH (4.2% severity) in blight control, however, this treatment enhanced grain yield significantly (1.241 t ha⁻¹) over three sprays of BTH (0.922 t ha⁻¹).     

Yield formation and tillering dynamics of direct-seeded rice in flooded and nonflooded soils in the Huai River Basin of China

Water shortage in the Huai River Basin prompts farmers to adopt water-saving technologies such as direct-seeded nonflooded or aerobic rice. Different cultivation practices impact on tiller growth and development. Improved insight into tiller dynamics is needed to increase yield in these production systems. We conducted field experiments with four direct-seeded rice varieties under flooded and nonflooded conditions in Mengcheng county, Anhui province, in 2005–2006. The soil water content in the nonflooded treatment varied between saturation and field capacity. Yields in nonflooded soil ranged from 3.6 to 4.7 t ha⁻¹, and did not differ significantly from yields in flooded soil that ranged from 3.6 to 5.1 t ha⁻¹. Variety had a significant effect on biomass, yield, panicle number, spikelet number, grain weight, and grain filling percentage. Panicle number was the main factor limiting yield, resulting from a low tiller emergence frequency and a low fraction of productive tillers in both the flooded and the nonflooded soils. On average, the panicle number was 159–232 m⁻², including 34–167 productive tillers per m² for all the varieties under the two water regimes. The contribution of productive tillers to yield varied between 7% and 47%. There were two peaks of tillers that contributed to yield, one at the low (4th or 5th) and one at the high (10th or 11th) phytomer orders. Frequencies of tiller emergence at most phytomer orders were higher in the flooded soil than in the nonflooded soil. There were no significant differences in frequencies of productive tiller emergence and contributions to yield from tillers between the soil water regimes for three of the four tested varieties. To increase yield in direct-seeded nonflooded rice production systems, both the tiller emergence frequency and the fraction of productive tillers should increase through breeding, improved crop management, or a combination.     

Identification and quantification of phenolic acids and anthocyanins as antioxidants in bran,embryo and endosperm of white,red and black rice kernels (Oryza sativa L.)

Whole rice grain comprising endosperm, embryo (or germ), and bran has potential beneficial health effects in addition to provision of nutrients. The distribution of phenolic acids and anthocyanins in endosperm, embryo, and bran of white, red, and black rice grains was investigated in this study. The total phenolic content (TPC) was highest in the bran, averaging 7.35 mg GAE/g and contributing 60%, 86% and 84% of phenolics in white, red and black rice. The average TPC of the embryo and endosperm were 2.79 and 0.11 mg GAE/g accounting for 17% and 23%, 4% and 10% and 7% and 9% in white, red and black rice, respectively. The antioxidant capacity determined using DPPH and ORAC displayed a trend similar to TPC. Free/conjugated phenolic acids in white, red and black rice bran accounted for 41%, 65% and 85% of total acids. Bound phenolic acids in rice bran accounted for 90% of total acids in whole grain. Cis-p-coumaric was detected in bound form in bran while cis-sinapic acid was detected in the free/conjugated form in embryo and bran. Cyanidin-3-O-glucoside and peonidin-3-O-glucoside were identified mainly in black rice bran as the total anthocyanins. Cyanindin-3-O-rutinoside was also detected in black rice bran.     

Evaluation of CIMMYT conventional and synthetic spring wheat germplasm in rainfed sub-tropical environments. II. Grain yield components and physiological traits

CIMMYT hexaploid spring wheat (Triticum aestivum L.) germplasm has played a global role in assisting wheat improvement. This study evaluated four classes of CIMMYT germplasm (encompassing a total of 273 lines), along with 15 Australian cultivars (Oz lines) for grain yield, yield components and physiological traits in up to 27 environments in Australia's north-eastern region, where terminal drought frequently reduces grain yield and grain size.Broadly-adapted CIMMYT germplasm selected for grain yield had greater yield potential and improved performance under drought stress, being up to 5% greater yielding in High-yielding (mean yield 429 g m⁻²) and 4-10% greater yielding than adapted Oz lines in Low-yielding environments (mean yield 185 g m⁻²). Whilst maintaining statistically similar harvest index and spikes m⁻² compared to broadly-adapted Oz lines across all environments, sets of selected CIMMYT lines had greater canopy temperature depression (0.18-0.27 °C), dry weight stem⁻¹ (0.20-0.37 g), increased grains spike⁻¹ (0.8-3.4 grains), grain number m⁻² (ca. 20-800 grains), and maturity biomass (56-83 g m⁻²). Compared to selected Oz lines, broadly-adapted CIMMYT lines had a smaller reduction in Low compared to High-yielding environments for these traits, especially dry weight stem⁻¹, such that CIMMYT lines had ca. 25% and 10% greater dry weight stem⁻¹ than the Oz lines in Low- and High-yielding environment groups, respectively. Broadly-adapted CIMMYT germplasm also had slightly higher stem water soluble carbohydrate concentration at anthesis (ca. 6 mg g⁻¹), which contributed to their higher grain weight (ca. 0.5 mg grain⁻¹), and maintained an agronomically appropriate time to anthesis and plant height. Thus current CIMMYT germplasm should be useful donor sources of traits to enrich breeding programs targeting variable production environments where there is a high probability of water deficit during grain filling. However, as multiple traits were important, efficient introgression of these traits in breeding programs will be complex.     

Spectral measurements of the total aerial N and biomass dry weight in maize using a quadrilateral-view optic

Heterogeneous crop stands require locally adapted nitrogen fertilizer application based on rapid and precise measurements of the local crop nitrogen status. In the present study, we validated a promising technique for the latter, namely a tractor-mounted field spectrometer with an oblique quadrilateral-view measuring optic, measuring solar radiation and canopy reflectance in four directions simultaneously. Dry matter yield (kg ha⁻¹), total N content (g N g⁻¹ dry matter) and total aerial N (aboveground N-uptake) (kg N ha⁻¹) in maize were determined in 10 m² calibration areas in 60 plots differing in their N treatment and seeding density three times in each of three years under field conditions. Results show that the sensor used can reliably determine total aerial N ranging from as little as 5 kg N to 150 kg N ha⁻¹ with R²-values ≥0.81 in 2002 and 2004, and with R²-values ranging from ≥0.57 to 0.84 in 2003. Dry matter yields from as low as 0.3–4.2 t ha⁻¹ could be determined with R²-values ranging from 0.67 to 0.91 in 2002 to 2004. The capacity to ascertain DM yield spectrally was drastically reduced in the higher yield range (>6 t ha⁻¹) probably due to decreased sensitivity of the spectral signal. N-contents were generally not well determined. Taken together there is a good potential to determine reliably differences in total aerial N or DM yield from the five leaf stages unfolded to the five node stage where typically nitrogen applications are carried out.     

Radiation use efficiency,N accumulation and biomass production of high-yielding rice in aerobic culture

The concept of aerobic culture is to save water resource while maintaining high productivity in irrigated rice ecosystem. This study compared nitrogen (N) accumulation and radiation use efficiency (RUE) in the biomass production of rice crops in aerobic and flooded cultures. The total water input was 800–1300 mm and 1500–3500 mm in aerobic culture and flooded culture, respectively, and four high-yielding rice cultivars were grown with a high rate of N application (180 kg N ha⁻¹) at two sites (Tokyo and Osaka) in Japan in 2007 and 2008. The aboveground biomass and N accumulation at maturity were significantly higher in aerobic culture (17.2–18.5 t ha⁻¹ and 194–233  kg N ha⁻¹, respectively) than in flooded culture (14.7–15.8 t ha⁻¹ and 142–173 kg N ha⁻¹) except in Tokyo in 2007, where the surface soil moisture content frequently declined. The crop maintained higher N uptake in aerobic culture than in flooded culture, because in aerobic culture there was a higher N accumulation rate in the reproductive stage. RUE in aerobic culture was comparable to, or higher than, that in flooded culture (1.27–1.50 g MJ⁻¹ vs. 1.20–1.37 g MJ⁻¹), except in Tokyo in 2007 (1.30 g MJ⁻¹ vs. 1.37 g MJ⁻¹). These results suggest that higher biomass production in aerobic culture was attributable to greater N accumulation, leading to higher N concentration (N%) than in flooded culture. Cultivar differences in response to water regimes were thought to reflect differences in mainly (1) early vigor and RUE under temporary declines in soil moisture in aerobic culture and (2) the ability to maintain high N% in flooded culture.     

Herbicide options for effective weed management in dry direct-seeded rice under scented rice-wheat rotation of western Indo-Gangetic Plains

Farmers' participatory field trials were conducted at Madhuban, and Taraori, the two participatory experimental sites/locations of the Cereal Systems Initiative for South Asia (CSISA), a collaborative project of IRRI and CIMMYT in Karnal district of Haryana, India, during Kharif (wet season) 2010 and 2011. This research aimed to evaluate preemergence (PRE) and postemergence (POST) herbicides for providing feasible and economically viable weed management options to farmers for predominant scented rice varieties. Treatments with pendimethalin PRE fb bispyribac-sodium + azimsulfuron POST had lower weed biomass at 45 days after sowing (DAS). At Madhuban, highest grain yield of scented basmati rice (3.43 t ha⁻¹) was recorded with the sequential application of pendimethalin PRE fb bispyribac-sodium + azimsulfuron POST. However, at Taraori, yields were similar with pendimethalin or oxadiargyl PRE fb bispyribac-sodium and/or azimsulfuron POST. Applying oxadiargyl by mixing with sand onto flooded field was less effective than spray applications in non-flooded field. The benefit-cost ratio of rice crop was higher with herbicide treatments at both sites as compared with the non-treated weed-free check except single PRE and POST applications and sequential application of oxadiargyl PRE fb oxadiargyl PRE. In a separate experiment conducted at Nagla and Taraori sites, scented rice cultivars' ('CSR 30′ and 'Pusa 1121′) tolerance to three rates of azimsulfuron (15, 25, and 35 g ai ha⁻¹) was evaluated over two years (2010 and 2011). CSR 30 (superfine, scented) was more sensitive to higher rates (35 g ai ha⁻¹) of azimsulfuron as compared to Pusa 1121 (fine, scented). Crop injuries were 8 and 28% in case of CSR 30; 5 and 15% in Pusa 1121 when applied with azimsulfuron 25 and 35 g ai ha⁻¹, respectively. Azimsulfuron applied at 35 g ai ha⁻¹ reduced yield in both cultivars but in CSR 30 yield reduction was twofold (11.5%) as that of Pusa 1121 (5.2%).     

Analysis of yield attributes and crop physiological traits of Liangyoupeijiu,a hybrid rice recently bred in China

Crop physiological traits of Liangyoupeijiu, a “super” hybrid rice variety recently bred in China, were compared with those of Takanari and Nipponbare in 2003 and 2004 in Kyoto, Japan. Liangyoupeijiu showed a significantly higher grain yield than Nipponbare in both years, and achieved a grain yield of 11.8 t ha⁻¹ in 2004, which is the highest yield observed under environmental conditions in Kyoto. Liangyoupeijiu had longer growth duration and larger leaf area duration (LAD) before heading, causing larger biomass accumulation before heading than the other two varieties. Liangyoupeijiu had a large number of grains and translocated a large amount of carbohydrates from the vegetative organ to the panicle during the grain filling period. The three yield components measured were panicle weight at heading (P₀), the amount of carbohydrates translocated from the leaf and stem to the panicle during the grain filling period (ΔT), and the newly assimilated carbohydrates during grain filling (ΔW). It was found that the sum of P₀ and ΔT were strongly correlated with grain yield when all the data (n = 8) were combined (r = 0.876**). However, there was no significant difference in the radiation use efficiency (RUE) of the whole growth period between Liangyoupeijiu and Nipponbare for both years. Even though the growth duration was shorter, Takanari, an indica/japonica cross-bred variety, showed a similar yield to Liangyoupeijiu in both years. The mean RUE of the whole growth period was significantly higher in Takanari, 1.60 and 1.64 g MJ⁻¹ in 2003 and 2004, respectively, than in Liangyoupeijiu, which had a RUE of 1.46 and 1.52 g MJ⁻¹ in 2003 and 2004, respectively. The high grain yield of Takanari was mainly due to its high RUE compared with Liangyoupeijiu and its large P₀ and ΔT. Our result showed that the high grain yield of Liangyoupeijiu was due to its large biomass accumulation before heading, which resulted from its large LAD rather than its RUE.     

Genotypic variation in linear rate of grain growth and contribution of stem reserves to grain yield in wheat

Grain growth in wheat depends on current photosynthesis and stem water-soluble carbohydrates (WSC). In semiarid regions with terminal drought, grain filling in wheat crops may depend more on stem WSC content than on current assimilates. Reduction in grain yield under drought is attributed to shorter duration of linear grain growth despite increased contribution of stem reserves to grain yield. The amount of stem reserves is measured either by changes in stem dry weight (indirect method) or by stem WSC content (direct method). Genotypic variation in the rate and duration of linear grain growth and in percent contribution of stem reserves to grain yield has not been evaluated in wheat. The objectives of this study were: (i) to quantify the relationship between the direct and indirect measurement of stem reserves during and across the grain-filling period and (ii) to measure the extent of genotypic variation in rate and duration of linear grain growth and in percent contribution of stem reserves to grain yield. Dry weight, WSC content and grain yield of the main stem were measured at 10-day intervals in 11 diverse wheat genotypes under well-watered and droughted-field conditions across 2 years. Drought reduced stem WSC content from 413 to 281 mg and grain yield from 4.6 to 2.5 t ha⁻¹. Stem WSC content and dry weight were positively correlated. Genotypic differences in linear rate of grain growth were significant in well-watered (ranging from 48.9 to 72.4 mg spike⁻¹ day⁻¹) and in droughted-field (ranging from 33.2 to 59.9 mg spike⁻¹ day⁻¹) conditions. Drought, on average, reduced the linear rate and duration of grain growth by 20 and 50%, respectively. Reduction in linear rate ranged from 13 to 43%. The amount of current assimilates and stem reserves contributed to grain yield was reduced, respectively, by 54 and 11% under drought. Genotypic differences in percent contribution of stem reserves to grain yield were significant in well-watered (ranging from 19.1 to 53.6%) and in droughted-field (ranging from 36.6 to 65.4%) conditions. The wheat genotypes responded differently to drought. Main spike grain yield was reduced by 43% under drought due to 26 and 11% reduction in grain weight and number of grains, respectively. Grain yield was correlated with linear grain growth under well-watered (r = 0.96) and droughted (r = 0.83) conditions. The genotypic variation observed indicates that breeding for a higher rate of linear grain growth and greater contribution of stem reserves to grain yield should be possible in wheat to stabilize grain yield in stressful environments.