旧膜及秸秆覆盖对西北旱地马铃薯 产量及土壤水热的影响

为了探究旧膜二茬及秸秆带状覆盖条件下,马铃薯田的土壤水热特征及增产机制,设置旧膜直播(T1)、秸秆带状覆盖种植带不旋耕（T2）、秸秆带状覆盖种植带旋耕（T3）、新覆膜（T4）和露地平作（CK）5个处理进行田间试验。结果表明：各覆盖处理较CK提高产量14.24%~56.33%、商品薯率1.21%~22.60%及水分利用效率8.28%~55.39%（P<0.05）;产量与块茎形成期的单株薯干重正相关（r=0.744）,覆盖处理在块茎形成期较CK提高单株薯干重118.0%~720.0%（P<0.05）,以T4最高;覆盖处理对马铃薯水热特征有显著影响（P<0.05）,T1、T2的降温效应显著小于T3,T3的蓄水保墒效应与T4差异不显著,但显著大于T1、T2及CK。可见,在本试验条件下,秸秆带状覆盖种植带旋耕的产量高于旧膜直播、略低于新覆膜,但蓄水保墒效应与新覆膜相近,同时秸秆带状覆盖种植带旋耕较其余覆盖处理具有较明显的降温增墒效应,有利于促进马铃薯块茎的形成及膨大。     

不同连作年限马铃薯根际土壤细菌群落特征研究

采用长期定位试验,设马铃薯连作1~7 a（CP1~CP7）和轮作（RT）8个处理,通过高通量测序探究不同连作年限对马铃薯根际土壤细菌群落多样性的影响。结果表明：供试土壤中,放线菌门、变形菌门、厚壁菌门为主要的优势菌门。随连作年限的增加,土壤细菌数量表现出波动变化趋势,连作年限影响细菌群落结构。RT、CP1、CP2、CP3处理土壤细菌相似度较高,CP4、CP5、CP6、CP7处理土壤细菌相似度较高。马铃薯连作7 a土壤中的放线菌门相对丰度降幅最大,较RT降低10.47%;厚壁菌门相对丰度在连作6 a土壤中显著降低,较RT减少22.07%;类诺卡氏属相对丰度在连作7 a显著减少,较RT减少2.34%;芽孢杆菌属相对丰度在连作6 a时最低,较RT减少9.18%;连作7 a较RT减少5.14%;芽单胞菌属相对丰度在连作7 a土壤中达到最高（16.54%）,较RT增加5.26%。长期连作改变了马铃薯土壤细菌群落结构,连作4 a土壤细菌群落种类开始变化,土壤细菌群落结构也随之改变。土壤中预防病害的菌类减少,增加了马铃薯染病风险。     

马铃薯长期连作对土壤真菌群落特征的影响

利用田间长期定位试验,2019年基于ITS高通量测序研究马铃薯/春小麦/碗豆轮作（R）、马铃薯连作1 a（C1）、3 a（C3）、5 a（C5）、7 a（C7）和9 a（C9）的土壤真菌群落结构的演替特征。结果表明：随着马铃薯连作年限的增加,土壤真菌菌群丰度和Chao1显著增加,C7和C9马铃薯土壤真菌菌群丰度最高,R土壤真菌菌群丰度最低,C7和C9真菌群落的相似度较高,C7、C9处理与R处理的真菌群落相似度低,C1、C3、C5的真菌群落结构介于C7、C9与R之间;土壤真菌群落的相似度在纲水平上分为两类,C7和C9为一组,R、C1、C3、C5为一组;在不同连作年限的马铃薯农田土壤中,共检测到273个真菌属,其中有13个真菌属处理间差异显著,其中,青霉菌(Penicillium)在C5、C7和C9土壤中数量显著高于R,绿僵菌属（Metarhizium）在C5、C7、C9中相对丰度显著高于R、C1、C3,镰刀菌属（Fusaruim）在C5中相对丰度达最高,显著高于R、C1、C3以及C7、C9;马铃薯连作5 a后引起农田真菌群落分布发生变化。青霉菌、绿僵菌属在长期连作农田中大量富集,镰刀菌属数量下降,连作障碍程度趋于减缓。     

Effect of crop rotation on Globodera rostochiensis and on potato yield1

A crop rotation field study was conducted in 1981–1989 to assess the effect of six crop rotation sequences on a soil population of potato cyst nematode (PCN), Globodera rostochiensis, and on potato yield. In the plots with potato monoculture, the tuber yield decreased from 35 to 4.6 t ha^?1. All other cropping systems maintained the yield at the original or an even higher level. In monoculture, the population density of the nematode was raised during the first 3 years from 0.1 to 265 larvae g^?1 soil, and the density fluctuated afterwards between 30 and 136 larvae g^?1 soil. Three of the cropping systems, (1) susceptible potato once in 5 years, (2) susceptible potato once in 4 years, and (3) potato once in 3 years using resistant (H1) and susceptible cultivars alternately, decreased the nematode density under the economic threshold or even under the level of detection. The nematicide treatments (oxamyl) did not control nematode multiplication but prevented yield losses. No nematicide is currently registered nor needed for the control of PCN in Finland. The nematode can be controlled effectively with crop rotation, resistant cultivars and early harvesting.     

Interactions Between the Potato Cyst Nematode Globodera rostochiensis and Diseases Caused by Rhizoctonia solani AG3 in Potatoes Under Field Conditions

Findings from 2 years of field experiments investigating the relationship between Globodera rostochiensis and Rhizoctonia solani on unique field sites are reported. In 2000, a field experiment was positioned on land that had previously been used for experimental work investigating integrated potato cyst nematode (PCN) management methods. This study had produced an ‘untypical’ mosaic of PCN population densities ranging from 5 to 221 eggs g⁻¹ soil. In 2001, the field experiment was conducted on a different field site and overlaid on a focus of G. rostochiensis population densities ranging from 11 to 108 eggs g⁻¹ soil. In each experiment, potatoes (cv. Désirée) were grown in plots with similar population densities of G. rostochiensis that were either uninoculated or inoculated with R. solani. A series of potato plant harvests were undertaken to investigate the effects of nematode infestation on the incidence and severity of R. solani diseases and the associated development of plants. In both experiments, a clear relationship was found between the density of G. rostochiensis juveniles present in potato roots and the incidence of stolons infected by R. solani, 6 weeks after planting. For the first time this interaction has been determined under field conditions. The results of the study suggest that the interaction between nematode and fungus is indirect and possible mechanisms are discussed.     

Monitoring and forecasting in plant protection in the German Democratic Republic

In the GDR, a regional pest monitoring scheme run by the State Plant Protection Service collects data on 87 pests for preparing national pest management warnings and recommendations and for strategic planning. Within each farm, plant protection specialists follow a field-level crop monitoring procedure to guide them in their immediate decision making. Regional 'standard values for control', oriented according to national economic requirements, are adjusted in the light of immediate local ecological and agrotechnical conditions into 'control thresholds' used for local decision making. Simulation models assist in fixing these control indices. In particular, SIMPHYT I and SIMPHYT II calculate the course of Phytophthora infestans epidemics and provide the basis for the PHYTEB forecasting procedure. These systems have been operational in GDR since 1982 and in general result in saving at least one spray per season. SIMPHYT III, a new system under trial, incorporates fungicide behaviour as well, SIMLEP I has been operational since 1983 and provides forecasts of Colorado beetle populations. Five other such systems are at an advanced stage of development. In orchards control decisions will instead be based on locally installed automatic microchip-based warning devices. These monitor temperature, humidity, leaf wetness and wind velocity and can be programmed for all the major orchard pests and diseases. Trials in five intensive orchards in 1982/84 have been given very promising results and it is suggested that one such device, representative of an area of 500–1000 ha, could save about 150,000 Marks per annum in reducing the number of 'insurance' sprays.     

The Basis of Predictive Modelling for Estimating Yield Loss and Planning Potato Cyst Nematode Management

The basis of modelling yield loss and population dynamics relations of potato cyst nematodes is that both are strongly density-dependent. Potato cyst nematodes (PCN; Globodera pallida and G. rostochiensis) are particularly suitable for analysing such relationships because they have only one generation per year, potato is their only field host, the juvenile nematodes within the egg are very durable (up to 20 years persistence), and they hatch mainly in response to specific chemicals exuded from host roots. Small populations increase the most, up to 50-fold, when a potato crop is grown. Multiplication rates decrease as the population density increases because damage decreases root system size and increases competition so that very large populations may actually be decreased when potatoes are grown. The newly formed eggs have a ‘half-life’ of c.two years when non-host, rotational crops are being grown. Control is achieved largely by the use of rotation, the application of nematicidal chemicals, and growing resistant cultivars. As rotations are shortened, so PCN populations will be increased, and crop damage becomes more likely. Thresholds for damage vary with both soil type (greatest on sandy soils) and potato cultivar. Cultivars differ in their tolerance of PCN damage depending on how vigorously they grow, on their root sensitivity to damage from the PCN juveniles which invade close to root tips, on cultivar resistance which decreases the parasitic effect by reducing the numbers of developing PCN females, and on various environmental factors such as the amounts of fertiliser applied. The dependence of the yield-loss relationship on population density, soil type and cultivar effects has been described in a simple equation and assessed using field-trial data. Effects on yield are described in proportion to the PCN-free yield but the addition of information on expected yield (in tonnes ha⁻¹) in the absence of PCN renders this equation predictive. Nematicides are widely applied to infestations of potato cyst nematodes, both to prevent the crop from being damaged and to prevent population increase which could hazard the next potato crop in the rotation, but they are generally more effective at preventing the former than the latter. A complex equation has also been developed to model the population dynamics of PCN. This equation incorporates a factor for host-crop growth and tolerance (from the yield-loss equation) and also the effects of host resistance. This latter is particularly relevant to G. pallida, where all the resistance currently available is determined by minor genes and hence is ‘quantitative’ or ‘partial’. Effects of rotation and of nematicides can also be incorporated into this model. To provide a realistic prediction also requires accurate information on PCN population densities, species composition and distributions, and rates of PCN population decline between potato crops.     

基于高光谱与电磁感应技术的干旱区绿洲土壤含水量反演研究

为实现干旱区绿洲土壤含水量的快速、准确监测,利用采集自渭干河-库车河绿洲的84个表层(0~10cm)土壤样本,通过利用电磁感应仪(EM38)将所测解译后数据代替实测土壤含水量数据,将高光谱反射率重采样为Landsat8卫星遥感波段反射率,在选取光谱特征参数、提取敏感波段的基础上,利用偏最小二乘回归(PLSR)方法建立土壤含水量模型,将最优估算模型应用于遥感影像,实现研究区土壤含水量遥感反演。研究结果表明:(1)利用EM38所测水平模式土壤表观电导率与土壤含水量拟合效果最优,能够代替实测土壤含水量进行后续建模分析。(2)相比3种单一的光谱特征指数,利用多种光谱特征指数所建土壤含水量估算模型的建模效果更优,其干、湿各季建模集决定系数R~2大于0.7,均方根误差(RMSE)均小于0.5%,RPD均大于2,能够作为有效手段估算干旱区绿洲土壤含水量。(3)不同季节土壤含水量遥感反演值与实测值决定系数R~2均大于0.6,均方根误差(RMSE)均小于0.6%,显示了较高的预测精度,证明利用电磁感应技术与高光谱相结合能够实现对干旱区绿洲土壤含水量的精准、高效监测。     

Simulation of herbicide persistence in soil .II. Simazine and linuron in long-term experiments

The rates of degradation of simazine and linuron were measured in soil from plots not treated previously with these herbicides. Degradation of both compounds followed first-order kinetics and soil temperature and soil moisture content had a marked effect on the rate of loss. With linuron, half-lives increased from 36 to 106 days with a reduction in temperature from 30° to 5°C at 4% soil moisture, and from 29 to 83 days at 12% soil moisture. Similar temperature changes increased the half-life of simazine from 29 to 209 days and from 16 to 125 days at soil moisture contents of 4 and 12% respectively. A computer program which has been developed for simulation of herbicide persistence was used in conjunction with the laboratory data and the relevant meteorological records for the years 1964 to 1968 in order to test the model against previously published field persistence data for the two herbicides. The results with simazine showed a close correspondence between observed and predicted residue levels but those for linuron, particularly in uncropped plots, were satisfactory for limited periods only.     

应用粒子滤波同化条件植被温度指数的土壤水分估测

为了准确地获取2013—2015年关中平原冬小麦主要生育期土壤含水量(0~20 cm)的时空信息,基于Landsat-8遥感数据反演条件植被温度指数(CVTI),并结合CVTI和实测土壤水分间的线性相关性构建土壤水分反演模型。应用粒子滤波(PF)算法同化基于CVTI反演的和CERES-Wheat模型模拟的土壤水分,得到以天为步长的土壤水分同化值,利用土壤水分实测值分别检验土壤水分模拟值、反演值和同化值的精度。结果表明,CVTI和实测土壤水分间的线性相关性显著,尤其在小麦拔节期和抽穗~灌浆期,其相关性达到极显著水平(P0.01);土壤水分同化值和实测值间的线性相关性(r=0.96,P0.001)大于土壤水分模拟值和实测值间的相关性(r=0.71,P0.01)以及土壤水分反演值和实测值间的相关性(r=0.89,P0.001);土壤水分同化值的均方根误差(RMSE)和平均相对误差(MRE)比土壤水分模拟值的RMSE和MRE分别降低了0.025 cm~3·cm~(-3)和2.70%,比土壤水分反演值的RMSE和MRE分别降低了0.016 cm~3·cm~(-3)和4.15%,同化过程提高了时间序列土壤含水量的估测精度。因此,基于CVTI和PF算法能够较为准确估测关中平原小麦主要生育期的土壤含水量。     

连续施用不同氮量对半干旱地区马铃薯根际真菌群落结构的影响

针对马铃薯生产中因氮肥过量施用导致的土壤微生物群落结构失衡和多样性下降等问题,在始于2013年的不同氮肥用量(N0:不施氮,对照;N75:施氮量75 kg·hm^-2;N150:施氮量150 kg·hm^-2;N225:施氮量225 kg·hm^-2;N300:施氮量300 kg·hm^-2;N375:施氮量375 kg·hm^-2)田间定位试验中,于2017年马铃薯成熟期采集根际土壤,应用Illumina PE250测序等分子生物学手段,研究连续5 a施用不同氮量对半干旱地区马铃薯根际真菌群落结构的影响。结果表明:不同施氮量对马铃薯根际真菌群落物种组成造成了显著影响,子囊菌门、Mucoromycota和担子菌门是3个优势门类真菌(相对丰度>1.0%),以子囊菌门的相对丰度最大,占总序列的75.48%～83.95%,其优势属是Plectosphaerella(29.92%)和镰刀菌属(13.54%);马铃薯干腐病和枯萎病的病原菌——镰刀菌属的相对丰度随施氮量增加呈增大的趋势。马铃薯...     

孙家岔流域坡面不同土地利用的土壤水分变化分析

以甘肃省兰州市孙家岔流域为研究对象,采用1982—1983年和2011年土壤水分实测资料,分析该流域坡面防护区不同土地利用土壤水分的年内、年际变化特征。结果表明,在黄土丘陵区小流域内,农坡和荒坡土壤水分的年内动态变化具有相同的规律,但农坡年内平均含水量要高于荒地。流域内降雨量的多少以及时空分配是土壤水分变化趋势的主要影响因子。土壤水分的变异程度随降雨量和土层深度的增加而减小,土壤深度在1.20~1.40 m内含水量受季节气候动态变化影响显著,土壤深度超过1.60 m内含水量在相同年度具有一定的时间稳定性。土质较好的荒地,选择适宜的农作物(如洋芋),可提高缓坡区(5°~15°)降水资源的有效利用率。     

Prediction studies supported by computer on potato late blight in central Anatolia in Turkey*

Prediction of potato late blight epidemics, caused by Phytophthora infestans (Mont) de Bary, was studied in three different villages of Bolu Province having large potato growing areas with the Winstel and Ullrich Schrodter models in the years 2002–05. During these years, a late blight outbreak was observed only in 2005 with the disease being less apparent in the other years. The Ullrich Schrodter model was found to poorly predict potato late blight epidemics in 2005. The Winstel model gave first warnings too early but correctly predicted late infections. Both the A1 and A2 mating types were found in Central Anatolia for the first time, in Bolu province.     

一种虫生真菌-赤色穗状菌Spicaria fumoso-rosea (Wize) Vassilijevsky的研究

在辽宁省发現一种寄生于柞蚕寄生蝇蛹体上的食虫菌,鑑定为赤色穗状菌[Spicaria fumoso-rosea (Wize) Vassilijevsky],是我国首次詳細观察記载。此菌容易培养,在馬鈴薯、甘薯块上,以及馬、羊粪混和腐植土上都可以生长发育良好。致病力強,自然寄生率为3.4—20.3％;接种感染寄生率为10—100％。此菌除寄生于柞蚕寄生蝇蛹外,还可寄生于粘虫、苹果食心虫及家蝇等。本菌孢子发芽所需相对湿度为98—100％,在98％以下不能发芽;土壤绝对含水量15—55％时发生良好,尤以35％为最适宜。最适温度为20—24℃。小区接种試驗結果:在地面撒菌粉让寄蝇蛆爬行钻入,寄生率为72.98％;在土深3与5厘米处撒菌粉,寄生率为40.24％和43.98％。     

Evidence for presence of the founder Ia mtDNA haplotype of Phytophthora infestans in 19th century potato tubers from the Rothamsted archives

Late blight remained a significant disease for potato growers in Europe long after the famine of the 1840s. Of the four mitochondrial haplotypes of Phytophthora infestans, only the Ia mitochondrial DNA (mtDNA) haplotype has been identified previously in infected potato leaves from famine‐era herbarium specimens collected in England, Ireland and Europe in the 19th century. Long‐term soil fertility experiments were conducted on potato between 1876 and 1901 in Rothamsted to investigate effects of combinations of organic manures and mineral fertilizers on disease and yield. This report identifies for the first time the same Ia mtDNA haplotype of P. infestans in three diseased tubers from 1877 from the long‐term Rothamsted trials, thus providing the earliest evidence of the presence of the founder Ia mtDNA haplotype of P. infestans in potato tubers in England. Soil amendments had a significant impact on disease and yield. A real‐time PCR assay was used to detect and quantify P. infestans in tubers. The level of pathogen DNA was greatest in tubers from highest yielding plots that received combinations of inorganic nitrogenous and mineral fertilizers and least in tubers from plots with organic farmyard manures or non‐nitrogenous mineral fertilizers. The Ia mtDNA haplotype was also confirmed from diseased potato leaves during the same time period. Thus, the founder Ia mtDNA haplotype survived in potato tubers after 1846 and was present over 30 years later in the UK.     

基于高分遥感影像的亚高寒草甸土壤水分含量反演

通过高分卫星遥感影像计算植被供水指数来反演亚高寒草甸土壤水分含量,结合高分辨率遥感影像(GF-2)和中分辨率的遥感影像(Landsat-7)进行土壤水分反演模型建模验证,揭示高分遥感影像结合植被供水指数法在青藏高原东北缘亚高寒草甸草原上的适用性,同时分析研究区土壤水分分布及其影响因素。基于高分二号（GF-2）、Landsat-7影像数据,以甘南藏族自治州当周草原为研究区,利用植被供水指数（VSWI, vegetation supply water index）构建土壤水分反演模型得到研究区土壤水分含量反演图,通过半方差函数及主成分分析法探索研究区土壤水分空间分布及影响因素。结果表明：研究区土壤水分含量分布状态呈现出一定程度的空间变异,体现在整个研究区内以及各个地块之间,土壤水分含量主要介于0.11%~60.44%之间;土壤水分含量与坡度、海拔、坡向、NDVI、地表温度均呈正相关关系,分布主要受NDVI、坡向、坡度、海拔的影响。综上,利用植被供水指数法结合高分遥感影像监测土壤水分含量是可行的,基于GF-2遥感影像所建立的模型拟合度最优,较Landsat-7遥感影像更具优势。     

Phytopathological situation in potato in Belarus1

The control of potato diseases in Ukraine is reviewed. Control of potato blight (Phytophthora infestans) has only been adequate for limited periods in the past (when resistant cultivars were available and when the systemic fungicides were introduced). Currently, the disease is in a new epiphytotic phase and control has failed again, probably connected with the introduction of new mating types and the existence of greater variability. Blight now occurs earlier in the season and in most years (instead of only years when climatic conditions are favourable). Control has now to be essentially preventive, rather than based on forecasting. The importance and control of other potato diseases is also considered (Alternaria spp., Rhizoctonia solani, Geotrichum candidum, Phytophthora erythroseptica, Sclerotinia sclerotiorum, Streptomyces scabies).     

Occurrence of Polyscytalum pustulans, Phoma foveata and Fusarium solani var. coemleum in field soils in Scotland

The effectiveness of various methods for detecting three fungal potato pathogens was compared with artificially infested soil, naturally infested tuber-borne soil and field soil. In the spring of 1985 and 1986 field soils from 30 farms in north-east Scotland were sampled just before planting a seed potato crop and 6 months after harvesting such a crop. The minimum statutory gap between crops is 5 years. Polyscytalum pustulans was recovered from 32 out of 60 field soil samples taken 6 months after harvest while from fields sampled in the spring before a potato crop was planted the fungus was isolated from 10 out of 30 soils in 1985 and five out of 30 in 1986. Phoma foveata was isolated from only one out of 60 pre-planting soil samples but Fusarium solani var. coeruleum was recovered from eight of these soils.
Microplant bait plants were grown over 3 years at an experimental farm near Edinburgh in various fields at different intervals after a previous potato crop. Contamination by P . pustulans was not related to interval after potatoes between 1 - 7 years. No contamination was recorded in fields where potatoes had not been grown for more than 30 years.     

Influence of abiotic factors,inoculum source,and cultivar susceptibility on the potato tuber blemish diseases black dot (Colletotrichum coccodes) and silver scurf (Helminthosporium solani)

Black dot and silver scurf are potato blemish diseases whose economic impact has increased in recent years. Because their symptomatology on tubers is visually similar, disease assessment does not usually differentiate between the two pathogens, which share the same ecological niche. The epidemiology of black dot has been extensively studied, especially in the UK, but the factors that influence silver scurf have been less investigated. In this study, the influence of cultivar, source of inoculum, and environmental conditions on both diseases was studied in field trials over a three-year period (2016–2018) in Switzerland. Planting minitubers did not prevent either disease in daughter tubers, indicating the contribution of soil as an inoculum reservoir. An arbitrary threshold of Colletotrichum coccodes soil inoculum could be set to discriminate between low and high disease risk. For the first time, Helminthosporium solani DNA was detected in stolons, and infections appeared earlier in stolons than in tubers. H. solani stolon and tuber infections usually appeared later in the season than those of C. coccodes. Black dot severity correlated positively with precipitation, while silver scurf severity correlated positively with temperature. Table potato cultivars commonly grown in Switzerland exhibited significant differences in susceptibility to both diseases, and cultivars with low susceptibility to both silver scurf and black dot were identified. These results gave new insights into understanding the factors driving the epidemiology of potato blemish diseases and may contribute to building a risk assessment scheme to manage both diseases simultaneously.     

Factors affecting glyphosate activity in Imperata cylindrica (L) Beau, and Cyperus votundus L. I. Effect of soil moisture

Established Greenhouse grown plants of cogongrass Imperata cylindrica (L) Beauv.) and purple nutsedge (Cyperus rotundusL.) were given three different soil moisture regimes; field capacity, moderate stress and extreme stress, followed 6 weeks later by glyphosate [(N-phosphonomeihyl) glydne] applications to the shoots at 0.2,0.4 and 0.8 kg/ha for Imperata and 0.3,0.6 and 1.12 kg/ha for Cyperus. Field capacity watering stimulated most vegetative growth in hoth species. Glyphosate given at field capacity decreased shoot dry weight in both species, and rhizome length, rhizome dry weight and total carbohydrate in Imperata and total number of tuber-bulbs in Cyperus. In contrast. at extreme soil moisture stress, glyphosate showed reduced activity which appeared to be related to the physiological and morphological behaviour of the plants arising from the drought trealment. Application of waier to the roots of the plants grown at soil moisture stress. I week before and I week afler glypbosate spraying, enhanced glyphosate activity, probably because of the recovery of processes disturbed by ibe soil moisture deficit.