郏县红牛疥螨病的治疗办法

牛疥螨病是牛养殖业中的常见疾病,主要疥螨寄生引起,是一种皮肤性疾病。发病之后皮肤溃烂、结痂、化脓,有奇痒的症状,会导致皮肤的增厚。随着牛养殖业的规模化发展,该病发生的概率逐年提升,长期患病会影响红牛的正常休息和采食,造成红牛体重的下降,影响郏县雪花牛肉的品质。     

郏县红牛结节性皮肤病的治疗办法

郏县红牛结节病是牛养殖业中的常见疾病,是由病毒引起郏县红牛的一种急性、亚急性或慢性、传染病。该病的临床特征是病牛发热、消瘦,淋巴结肿大,皮肤、水肿、局部形成坚硬的结节或溃疡为主要特征。     

丹麦红牛改良郏县红牛效果分析

丹麦红牛以产奶量、乳脂率、乳蛋白率高及产肉性能好而闻名于世。该牛毛色紫红或红色,胸部宽深,背腰平直,后躯宽广,乳房发育良好,耐粗饲,适应性强,是著名的改良牛品种。 郏县红牛是我国八大良种黄牛品种之一,主产于河南省平顶山市的郏县、宝丰和鲁山3县,分布于毗邻的10余个县（市）、区,现存栏约28万余头,占全市牛存栏量的38%。具有被毛紫红,体质结实,结构匀称,抗病力强,繁殖率、挽力大,耐粗饲等特点,但肉用性能欠佳。在保持郏县红牛优良特性的前提下,为了增大郏县红牛体格,提高载肉量和商品率,遵照以本品种选育为主,适当导入外血（1/2～1/4）杂交改良的选育方向,制定了导入丹麦红牛冻精颗粒改良郏县红牛的实施方案,使郏县红牛由役肉兼用型向肉役兼用型方向发展。     

关于郏县红牛产业发展的思考

本文对郏县红牛的生态环境、品种特征、发展现状进行了分析,对其发展意义进行了论述,并提出了建设性建议.     

郏县红牛同期发情及超数排卵的研究

[目的]探讨生产中郏县红牛同期发情及超数排卵的方法。[方法]筛选75头郏县红牛分组分批进行同期发情及超数排卵。[结果]显示,CIDR+PG法处理组母牛同期发情率(100%)显著高于PG二次法处理组(76%)(P0.05);用加拿大促卵泡素(FSH)超排时头均获胚数6.33枚,用中科院动物所促卵泡素(FSH)超排时头均获胚数为6.00枚,二者差异不显著(P0.05)。[结论]分析结果可知,CIDR+PG法处理比PG二次法对郏县红牛的同期发情效果好;进口促卵泡素(FSH)与国产促卵泡素(FSH)对郏县红牛的超排效果无显著差异。     

郏县红牛育种模式探讨

本文在总结郏县红牛２０年保种选育经验基础上，根据市场发展趋势及生物技术水平，提出了建立活体保种冷精胚胎保种相结合珠保种体系的技术措施，重点探讨了通过引入外血提高郏县红牛早熟性，泌乳性，肉用性的育种技术模式。     

郏县红牛的保种与开发利用

从郏县红牛的品种特性、发展历史出发,客观地分析了郏县红牛保种的必要性,根据保种选育工作进展情况,详细制定了郏县红牛的保种育种技术方案,并对如何开发利用这一品种资源进行了探讨。     

郏县红牛遗传育种研究进展

为了研究郏县红牛遗传育种研究进展,本文梳理了夏洛来牛、南德温牛、利木赞牛、红安格斯牛、丹麦红牛改良郏县红牛的研究进展,对郏县红牛基因多态性与生长性状的关系和基因遗传变异与生长性状的关系进行了梳理,结果表明：红安格斯牛可以提高郏县红牛的肉用性能和优质牛肉肉块重量,夏洛来牛改良郏县红牛可以提高牛肉品质;ZAG基因、IGF2基因、DGAT2基因、CLPG基因、AQP7基因可以作为生长性状辅助选择的基因,POU1F1基因第6外显子被Hinf I酶切后表现多态性,BB基因型个体可以作为生长性状辅助选择的基因,SREBP-1c基因内含子7区域84 bp缺失位点可以作为生长性状辅助选择的位点。     

郏县红牛发展中存在的主要问题及对策

郏县红牛发展中存在的主要问题及对策郑二欣耿二强张杰张俊英朱永红杨长松张利康（河南省平顶山市畜牧局４６７０００）（河南省鲁山县红牛场４６７３００１郏县红牛的发展成就１．１郏县红牛的特点郏县红牛是我国著名的役肉兼用型地方优良黄牛品种。具有被毛红色,体形匀...     

郏县红牛种质资源个性描述

通过查阅文献、现场调查、取样分析,对郏县红牛的基本情况、群体情况、生理生化指标、种质基本特征描述、体尺体重、繁殖性能、生产性能、遗传指标、饲养管理等方面对郏县红牛种质资源进行个性描述.在对郏县红牛种质资源进行评估的基础上,提出郏县红牛的研究开发及主要利用方向.     

中草药防治母牛不孕症及其作用机理

养牛业作为农业产业之一,肉、奶等产品在市场上的需求不断提升,但在养殖过程母牛患上生殖系统疾病,该病可引发发情期紊乱等疾病,如不有效治疗会严重影响母牛正常繁殖,养殖场效益受到影响。母牛生殖系统疾病治疗需根据临床诊断采取合理的方案进行,中草药治疗该病在临床上效果明显,本文通过采用中药对母牛生殖系统疾病进行,并阐述相关的作用机理,以供同行参考。     

山东省减抗背景下的中兽药产业调研分析

基于对山东省兽药生产企业和养殖企业中兽药生产和使用情况的调研,本文深入分析了我省中兽药发展现状和存在的问题,针对性地提出了中兽药产业发展的对策建议。需着力构建全产业链发展格局;不断释放市场创新创业活力;快速实现我省中兽药产业现代化;充分发挥好政策对产业引领作用;着力打造专业化人才队伍。并不断推进兽用抗菌药减量化示范行动等综合措施,从根本上遏制兽用抗菌药滥用,提倡使用中兽药等绿色高效产品的减抗行动,以期降低细菌耐药性的发生和发展,保障畜禽产品质量安全。     

犊牛常见病的诊治体会

犊牛的健康关系着牛场的未来生产。本文介绍了几种犊牛常见病的辨别及诊断方法,帮助牛场快速进行疾病判断。作者对几种疾病的病因、临床症状、诊断方式、治疗方法进行综述,旨在为犊牛的常见病的防控和选择治疗药物提供参考依据。     

重组犬瘟热病毒F-H融合基因乳酸菌的构建及表达

为了构建重组犬瘟热病毒(CDV)F-H融合基因工程乳酸菌,采用重叠延伸PCR(SOE-PCR)技术扩增CDV F-H融合基因。将F-H融合基因亚克隆至穿梭载体p SIP409多克隆位点上,构建p SIP-F-H表达重组子,并电转化至植物乳杆菌NC8感受态细胞中。利用SDS-PAGE和Western blotting检测F-H融合蛋白的表达情况。结果表明,成功地扩增了CDV F-H融合基因,构建了p SIP-F-H重组表达质粒,并电转化至乳酸杆菌感受态细胞中。SDS-PAGE和Western blotting检测表明,重组乳酸杆菌表达了分子量约为62.96 ku的F-H融合蛋白,且该蛋白能与CDV阳性抗体反应,具有反应原性。     

Effectiveness of maifanite in reducing the detrimental effects of aflatoxin B1 on hematology,aflatoxin B1 residues,and antioxidant enzymes activities of weanling piglets

A feeding trial was conducted to evaluate the effectiveness of maifanite, which is mainly composed of aluminosilicate, in reducing the adverse effects of aflatoxin B₁ (AFB₁) on hematology, AFB₁ residues, and antioxidant enzymes activities in weaning piglets. A total of 32 (9.28±0.17 kg BW) individually housed crossbred piglets (Duroc×Landrace×Large white) were randomly allotted to 1 of 4 dietary treatments in a 2×2 factorial arrangement with 8 replicates per treatment. The dietary treatments included 2 AFB₁ levels (5.3 and 372.8 μg/kg) and 2 maifanite levels (0% and 1%). No differences in average daily gain, average daily feed intake, and gain to feed ratio were observed among treatments. Ingestion of the AFB₁-contaminated (AF) diets (372.8 μg/kg of AFB₁) reduced (P<0.05) the numbers of neutrophil, monocyte, and total leukocyte. There were no effects of AFB₁ on gamma glutamyltransferase, alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase activities, and total protein, albumin, urea N, total bilirubin, IgG, IgA, and IgM concentrations in serum. Aflatoxin B₁ was found in the liver and kidney of piglets fed the AF diets. Piglets fed the AF diets reduced (P<0.05) the total superoxide dismutase, glutathione peroxidase, and catalase activities in serum. However, total superoxide dismutase activity in the liver was increased (P<0.05) in piglets fed the AF diets compared with those fed the basal diets (5.3 μg/kg of AFB₁). There was an interaction (P=0.003) in erythrocyte, but piglets fed the diets containing maifanite had greater erythrocyte and lymphocyte (P=0.035) numbers than those fed the diets without maifanite. The AFB₁ levels in the liver and kidney of piglets fed the AF diet containing maifanite were 29.6% and 41.2% lower, respectively, than those of piglets fed the AF diet alone. Although there was an interaction (P=0.011), piglets fed the diets containing AFB₁ and maifanite had greater serum T-SOD activity compared with those fed the diets with no maifanite. In conclusion, the addition of maifanite to the AF diet resulted in partial restoration of hematology and antioxidant enzymes activities and reduced AFB₁ levels in the liver and kidney.     

Effect of sample handling on venous PCO2, pH, bicarbonate, and base excess measured with a point-of-care analyzer

Objective: The purpose of this study was to determine the effect of timing of analysis, collection tube type and repeated opening of sample tubes on venous PCO₂, pH, HCO₃, and base excess (BE) results. Design: Prospective experimental study, paired sample analysis. Setting: Veterinary Medical Teaching Hospital. Animals: Twenty dogs. Interventions: Jugular venous blood samples. Measurements and main results: PCO₂, pH, HCO₃, and BE were determined immediately following collection (control) and at selected times up to 30 minutes after placement in either screw top or vacuum heparin collection tubes. A different set of screw top and vacuum heparin collection tubes were sampled repeatedly over time for up to 15 minutes. In the screw top delayed analysis group, only pH changed significantly at one time point. PCO₂ decreased significantly in all other groups and resulted in a significant reciprocal pH change in the vacuum tubes with either delayed single analysis or repeated sampling. HCO₃ and BE declined significantly in multi‐sampled vacuum tubes and HCO₃ also decreased significantly in multi‐sampled screw top tubes. Conclusions: Analysis of acid–base status is optimally performed on freshly drawn blood. However, when it is anticipated there will be a delay in analysis of samples kept at room temperature, the use of 2.0 mL plastic screw top heparin anticoagulant tubes may result in fewer pre‐analytical errors than 3.5 mL glass vacuum tubes.     

Effects of GnRH in combination with PGF2α on the dynamics of follicular and luteal cells in post-pubertal Holstein heifers

Holstein heifers were randomly allotted by weight, age and body condition score to one of three treatments to test the hypothesis that GnRH administration concurrent with PGF_2α injection would advance follicle or corpus luteum (CL) development parallel to an induced luteolysis of the pre-existing CL. Heifers in the control group (n = 14) received two treatments of PGF_2α(25 mg, im) given 10 days apart. Groups 2 (n = 14) and 3 (n = 14) received an additional treatment of GnRH (100 μg, im) after the first and second PGF_2α respectively. Estrus detection began immediately after PGF_2α and continued for 80 h. Blood sampling was initiated 7 days prior to the first PGF_2α (day − 7) and continued on days 0, 7, 10 (prior to the second PGF_2α), 17 and 24. Heifers were artificially inseminated after the second PGF_2α and pregnancy diagnosed at 60 days. There was a trend (P < .10) toward a lower estrus response in group 3 when compared to the other groups. Pregnant heifers in group 2 had lower progesterone (0.44 ± 0.09 vs. 1.72 ± 0.56 ng/ml) a week after the second PGF_2α than the non-pregnant animals in that group (P < .05). Similar results were observed in the control group but only within the responding heifers (0.61 ± 0.08 vs. 0.93 ± 0.03 ng/ml; P < .05). Progesterone in heifers in group 2 remained high on day 0, 7, and 10 (1.48 ± 0.37, 1.23 ± 0.39, 1.96 ± 0.36 ng/ml) in spite of the treatment with PGF_2α. This data suggest that administration of GnRH following PGF_2α alters bovine luteal and/or follicular cell function.     

非繁殖季节中国美利奴羊血清E2、P4、FSH、LH激素变化规律研究

为了在非繁殖季节判断绵羊的发情状况,试验以新疆高海拔地区中国美利奴羊为研究对象,采用酶联免疫测定(ELISA)法测定绵羊血清中雌二醇(E2)、孕酮(P4)、促卵泡素(FSH)、促黄体生成素(LH)的含量,研究激素诱导发情羊、发情症状不明显羊、不发情羊体内激素分泌规律的差异。结果表明:激素诱导发情羊的E2含量比不发情羊高,差异显著(P<0.05);但是在P4、FSH、LH水平上,激素诱导发情羊要低于不发情羊,差异不显著(P>0.05)。发情羊与发情不明显羊在4种激素含量上差异不显著(P>0.05),说明公羊试情存在一定缺陷,而通过测定E2含量判定绵羊发情状态将成为未来研究的重点。