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采用双抗体夹心酶联免疫吸附法(DAS-ELISA)和RT-PCR法,对甘肃省农业科学院蔬菜研究所试验地的辣椒病毒病病原进行检测。结果表明:在72份病样中,烟草花叶病毒(Tobacco mosaic virus,TMV)的总检出率为66.7%,没有检出黄瓜花叶病毒(Cucumber mosaic virus,CMV)。设计3对TMV引物对阳性样品进行扩增,其中引物TMV1扩增出相似片段目的条带,经BLASTN比对与番茄斑驳花叶病毒(Tomato mottle mosaic virus,To MMV)的序列一致性为97%。针对To MMV设计3对引物进行扩增,均扩增出单一且与预期大小相同的目标条带,经测序后与To MMV的序列一致性为98%以上,证明发生在甘肃省农业科学院蔬菜研究所试验地的辣椒病毒病病原为番茄斑驳花叶病毒。 相似文献
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河南番茄褪绿病毒的分子鉴定 总被引:1,自引:0,他引:1
2014年在河南省郑州、济源、新郑、中牟、扶沟等地发现大量保护地番茄植株的叶脉间出现褪绿症状,疑似由番茄褪绿病毒(Tomato chlorosis virus,To CV)侵染引起。以这5个地区的番茄病叶为样本提取总RNA,利用ToCV HSP70h(Heat shock protein 70 homolog,热激蛋白70家族)基因的特异引物对样本进行RT-PCR检测,均扩增出约460bp大小的目的条带。PCR产物测序和序列分析结果表明,该序列与国际上报道的ToCV HSP70h的序列相似性达到99%以上,表明ToCV是为害河南省番茄产区造成褪绿症状的主要病原之一。 相似文献
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对小西葫芦黄花叶病毒(ZYMV)、西瓜花叶病毒(WMV)、黄瓜花叶病毒(CMV)、番木瓜环斑病毒西瓜株系(PRSV-W)和南瓜花叶病毒(SqMV)人工接种侵染7种葫芦科作物后的症状反应进行了初步的观察和探讨,初步筛选出SqMV与南瓜(耳突)、西葫芦(锯齿状叶缘),ZYMV与西葫芦(蕨叶),丝瓜(锯齿状叶缘),CMV与丝瓜(子叶早期褪绿斑)、西葫芦(后期叶片坏死斑)等几种具有比较鉴定作用的植物与病毒的组合。 相似文献
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在对云南省西双版纳州进行病毒病害调查时发现1株疑似菜豆金色花叶病毒属病毒侵染的凤仙花植株,其症状表现为叶片皱缩和花叶。利用菜豆金色花叶病毒属病毒简并引物进行PCR扩增、克隆和分析,获得YN6198-28和YN6198-31两条菜豆金色花叶病毒属病毒的DNA-A全长序列,长度均为2 763 bp,编码6个开放阅读框,其中YN6198-28核苷酸序列与越南丁香黄脉病毒(Ludwigia yellow vein Vietnam virus,LuYVVNV)相似性为94.90%,表明该分离物是LuYVVNV1个分离物。重组分析发现,YN6198-28是种间重组病毒,其主要亲本为丁香黄脉病毒(Ludwigia yellow vein virus,LuYVV),次要亲本为中国胜红蓟黄脉病毒(Ageratum yellow vein China virus,AYVCNV)。 相似文献
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利用3对番茄褐色皱纹果病毒(Tomato brown rugose fruit virus,ToBRFV)特异性引物对云南红河、宁夏银川、北京顺义疑似病毒病样本进行RT-PCR检测,分别扩增出大小为812、842、1 053 bp的特异性条带。对扩增产物进行测序及系统进化分析,发现云南、宁夏、北京的ToBRFV分离物与约旦、意大利、荷兰的ToBRFV分离物聚在同一分支上,亲缘关系较近。致病性结果显示,接种后的番茄植株表现出与田间相同的症状,且在发病组织中检测到病毒ToBRFV。表明来自云南红河、宁夏银川、北京顺义的番茄植株均被ToBRFV侵染。 相似文献
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新疆哈密瓜病毒的DAS-ELISA检测和分子鉴定 总被引:7,自引:0,他引:7
为了明确当前新疆哈密瓜病毒病的主要毒原种类及其遗传分化,为哈密瓜病毒病害的防治提供科学依据,对吐鲁番、鄯善、昌吉等哈密瓜主产区的病毒病调查、采样,采用双抗体夹心酶联免疫吸附法(DAS–ELISA)和RT-PCR技术对侵染哈密瓜的7种主要病毒及瓜类重要检疫性病毒--黄瓜绿斑驳花叶病毒(Cucumber green mottle mosaic virus,CGMMV)进行检测和分子鉴定。结果表明:在所检测的121个样品中,黄瓜花叶病毒(Cucumber mosaic virus,CMV)的检出率最高,为70.2%;西瓜花叶病毒(Watermelon mosaic virus,WMV)和小西葫芦黄花叶病毒(Zucchini yellow mosaic virus,ZYMV)次之,分别为62.0%和37.2%,甜瓜坏死斑点病毒(Melon necrotic spot virus,MNSV)的检出率仅为2.5%,CGMMV、南瓜花叶病毒(Squash mosaic virus,SqMV)、番木瓜环斑病毒(Papaya ring spot virus W,PRSV-W)和烟草坏死病毒(Tobacco necrosis virus,TNV)未检测到。CMV、WMV、ZYMV在田间分布广泛,2种及3种病毒的复合侵染发生普遍(60.19%)。对各病毒分离物进行CP基因扩增、序列测定和系统发育分析,确定了CMV新疆哈密瓜分离物归属于CMV亚组ⅠB。ZYMV、WMV和MNSV与已报道的病毒株系CP基因核苷酸序列具有较高的同源性,但存在一定的变异,ZYMV和WMV具有明显的地域分化现象。 相似文献
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对鉴定不同菜豆品种真实性和种子纯度的田间小区种植法、种子形态法、电泳谱带法和分子标记等鉴定方法进行了综述,并提出可行性建议,旨在为准确、快速地鉴定菜豆品种真实性及种子纯度提供参考。 相似文献
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生长调节物质对高温下菜豆叶片水分的调节作用 总被引:1,自引:0,他引:1
在实验室控制高温条件下,分别用不同浓度的外源GA3和6-BA涂抹菜豆叶片,探讨它们对高温下菜豆叶片水分的调节作用及菜豆高温伤害状况,结果表明:在短期高温胁迫条件下,两种生长调节物质明显地提高了菜豆叶片细胞间的孔隙度,降低了高气温下植株的叶温,减缓了叶片细胞膜伤害;但是从总体上看两种调节物质处理后对叶片气体扩散阻力影响不大。 相似文献
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Ingrid H. Williams J. B. Free 《The Journal of Horticultural Science and Biotechnology》2013,88(4):405-413
Early flowers of runner bean plants set relatively fewer pods than those produced later. This difference can be only partly rectified by increased cross-pollination. 相似文献
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《Scientia Horticulturae》2005,107(1):2-10
Common bean is one of the most cultivated species in the Leguminosae family. Thus far, progress in bean improvement has been achieved mainly by conventional breeding methods. These methods, however, in addition of being time-consuming and labor-intensive, are met with a wide range of problems including traits associated with low genetic variation, low survivability of interspecific hybrids, specific inheritance of some valuable characteristics such as yield, disease, and pest resistance, as well as harvesting characteristics. Plant biotechnology offers different strategies to overcome these difficulties. With some exceptions, species belonging to the Leguminosae are difficult to regenerate in vitro. In this review, we discuss the potential and limitations of in vitro cultivation of Phaseolus vulgaris L. based on the existing literature data. Different tissue culture methods like somatic regeneration are discussed and evaluated as well as gene transfer and other approaches in bean modification in vitro. 相似文献
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对10个露地栽培的蔓生菜豆品种的抗性、产量及商品特性等进行考察比较,旨在筛选出适于昆明地区栽培的菜豆品种,为该地区菜豆的种植和推广提供依据。试验结果表明,泰国架豆王(黑皮、桂林天宇)、特级泰国架豆王和泰国架豆王(重庆华渝)3个品种的综合性状表现较好,产量较高,每667 m2分别达到了4 148.7,3 957.9,3 891.3 kg,比较适宜昆明地区种植推广。 相似文献
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菜豆种质资源的植物凝集素含量测定与评价 总被引:2,自引:0,他引:2
以112份菜豆种质商品荚为试验材料,测定了植物凝集素含量,分析评价其遗传变异,扩大极端菜豆凝集素水平的筛查范围。试验结果表明,供试菜豆种质的凝集素均值为0.335 3 mg/g,变幅为0.000 3~1.607 8 mg/g,变异系数为82.26%,种质间水平差异极显著(p0.01)。研究还表明,供试菜豆植物凝集素含量的种质频率分布曲线呈非连续分布但有明显主峰,有极低和极高水平的种质分布。在已检测的289份资源中,筛选出极低植物凝集素的菜豆材料2份,为低毒菜豆遗传改良提供技术基础。 相似文献
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R.C. Hardwick 《The Journal of Horticultural Science and Biotechnology》2013,88(3):395-410
The results are reported of an investigation into the effects of sowing on different dates on the emergence and growth of various cultivars of Phaseolus vulgaris and P. coccineus under field conditions. In 1969 five cultivars of French bean and three of runner bean were sown in the field at fortnightly intervals from April onwards. The experiment was repeated, with the addition of two cultivars, in 1970. Consistent differences were found between and within species for a number of growth parameters.(1) Especially in the early sowings, seedlings of runner beans emerged before those of French beans, and small-seeded cultivars of French bean (in particular Comtesse de Chambord) before larger-seeded cultivars.(2) The mean relative growth rates measured after emergence were greater in French beans than in runner beans, but differences within species were not significant. Very early sowing appeared to have a lasting depressive effect on the growth rates of seedlings of both species.(3) The estimated weights of seedlings at emergence (estimated from regressions of seedling weight on time since emergence and on seed weight) were lower in the early sowings than in later sowings, and especially so in the cultivar Comtesse de Chambord.The main results in each year were similar, but some differences in detail were found. In particular there were differences between years in the emergence of cultivars as affected by soil temperature. The discrepancies were attributed to a difference in the temperature regimes of the two sites. 相似文献