卵黄抗体的特点及在兽医学中的应用

传统制备抗体的方法,一般都是从啮齿动物、兔子或山羊等血清中提取抗体。用抗原免疫禽类,可以从其卵黄中提取特异性卵黄抗体,卵黄抗体与传统动物抗体的特点相比具有明显的优势,同时避免动物采血的痛苦,提高了动物福利。随着蛋白纯化技术的发展,卵黄抗体的应用范围已越来越广泛,在动物疾病预防控制、诊断和治疗中都发挥着重要的作用。     

在动物疾病防治中卵黄抗体应用探究

现阶段,生物科学领域的一项重大发现即为卵黄抗体,由于其自身具有相对简单的制备过程,因此获得较为广泛的应用.其制取过程主要是在母鸡体内注射一种特定的抗原物质,特定抗原会受到母鸡免疫系统的影响完成处理,这样的化学反映在经过免疫之后便会产生抗体,并在卵黄中集中起来,因而形成卵黄抗体.研究表明,卵黄抗体在动物疾病防治中扮演着重要性角色,对其加以广泛性应用研究,有利于促进动物疾病防治效果的提升.     

动物疾病防治中卵黄抗体的应用探究

当前,提到卵黄抗体,大家并不会感到十分陌生,卵黄抗体是当今生物科学领域的一大重要发现,其制备过程简单说来就是在母鸡体内注射特定的抗原后,母鸡体内的免疫系统就会对抗原进行处理,发生免疫化学反应,从而产生抗体,并且这些抗体会聚集在卵黄中,从而形成卵黄抗体。因此卵黄抗体具有制作简单方便、产量高、成本低、有较高的敏感性以及无副作用等众多优点,因此卵黄抗体在动物疾病的诊断、治疗以及预防中得到了广泛的应用。本文就针对动物疾病防治中卵黄抗体的应用进行了相关的探究。     

卵黄免疫球蛋白的应用进展

卵黄免疫球蛋白是一种来源丰富的多克隆抗体。由于卵黄抗体不能激活哺乳动物的补体系统,不与哺乳动物的Fc受体、类风湿因子、葡萄球菌蛋白A和葡萄球菌蛋白G结合,因而作为诊断试剂具有较高的敏感性。卵黄抗体具有良好的免疫活性,作为防治药物具有见效快,疗效高,无毒副作用,无残留等优点,目前卵黄抗体已得到了广泛应用。本文综述了卵黄抗体在动物病毒性疾病、细菌性疾病和寄生虫病诊断、预防和治疗等方面的应用及前景。     

卵黄抗体的特性及在鸭病防治上的应用

卵黄抗体是禽类经特异性抗原刺激由B淋巴细胞产生并移行至卵黄中的特异性抗体。卵黄抗体具有制作简单方便、性质稳定、产量高和成本低等多种优点,在动物疾病的诊断、治疗和预防等方面有着广泛的应用。本文就卵黄抗体的特性及在鸭病防治中的应用情况进行综述。     

卵黄抗体在水产动物疾病防治中的应用

<正>卵黄抗体又称卵黄免疫球蛋白(Ig Y),一般以鸡作为供体,经一种或多种特定抗原免疫诱导后在卵黄中蓄积,机体所产生的特异性抗体,具有类似哺乳动物Ig G的免疫活性,可有效防治特定疾病。近年来,在畜禽疾病的诊断与防治方面卵黄抗体的运用已非常广泛,可将其作为抗生素替代品或饲料添加剂等进行使用,在动物养殖业中具有广阔的应用前景。目前,用卵黄抗体防治水产动物疾病的研究报道较少,但因其具有疗效确切、使用安全、无毒副作用、制备工     

卵黄抗体在疾病防治中的应用

卵黄抗体(immunoglobulin of yolk,Ig Y)指从免疫禽蛋中提取出的针对特定抗原的抗体,是存在于卵黄中的最主要免疫球蛋白,具有特异性强、化学性质稳定、产量高、成本低以及动物种系发生距离远的优势,且无任何毒副作用,已被广泛应用于畜禽疾病及人类疾病的防治中。本文阐述了卵黄抗体的优势性质和结构特点,以及卵黄抗体在动物疾病、人类疾病及人兽共患病防治等方面的应用及发展前景。     

卵黄抗体的特性及在兔病防治上的应用

卵黄抗体是禽类经特异性抗原刺激由B淋巴细胞产生并移行至卵黄中的特异性抗体。卵黄抗体具有制作简单方便、性质稳定、产量高、成本低等优点,在动物的疾病诊断、治疗、预防等方面有着广泛的应用。本文就卵黄抗体的特性及在兔病防治中的应用情况进行阐述。     

卵黄抗体及其在疾病防制与饲料中的应用研究进展

卵黄抗体（immunoglobulin of yolk,IgY）具有性质稳定、制备简单、产量高、成本低等优点,已广泛用于动物的疾病防制,随着抗生素的禁用,卵黄抗体逐渐成为一种新型饲料添加剂在饲料中添加应用。作者就卵黄抗体的特征及其在动物疾病防制与饲料中的应用进行了简要概述。     

卵黄抗体在猪病防治上的应用

卵黄抗体是禽类经特异性抗原刺激由B淋巴细胞产生并移行至卵黄中的特异性抗体。卵黄抗体具有制作简单方便、性质稳定、产量高、成本低等多种优点,在动物的疾病诊断、治疗、预防等方面有着广泛的应用。文章就卵黄抗体在猪病防治中的应用情况进行综述。     

胶体金免疫层析技术在动物病毒性传染病诊断中的应用

自胶体金标记技术问世以来,已在妊娠检测、传染病、毒品、食品安全和兽医等领域得到了广泛的应用.就兽医领域而言,已应用在动物寄生虫、细菌、病毒以及兽药残留检测等方面.论文总结了胶体金免疫层析技术的基本原理和常见的检测抗原、抗体的方法,对胶体金免疫层析技术在动物病毒抗原和抗体检测中的应用进行了详细的介绍,并对该技术在动物病毒...     

Research Progress on Immunoglobulin of Yolk in the Prevention and Treatment of Various Diseases

Immunoglobulin of yolk (IgY) refers to the specific antibodies from the immune eggs, is the most important immunoglobulin in the yolk,which has the characteristics of stable chemical properties, high yield, low cost, high specificity and no toxic side effects, there was no cross serological reaction among animals breeds because of a long distance. IgY has a significant advantage in animal and human disease prevention and treatment. In this research,we reviewed the application of structural characteristics, physicochemical property and action mechanism of IgY in prevention treatment of animal disease and developing prospect, especially in the treatment of animal diseases, such as diarrhea and acute treatment of animal spirit, harm, large virus disease; zoonosis seriously endanger the safety of human property; Other aspects such as aquaculture, animal fur and other major diseases. IgY could effectively prevent and treat animal diseases, but also reduce the possibility of zoonosis, which will be of great significance for the protection of personal safety.     

卵黄抗体在疾病防制中的应用研究进展

卵黄抗体（immunoglobulin of yolk,IgY）是指从免疫禽蛋中提取出的针对特定抗原的抗体,是存在于卵黄中的最主要免疫球蛋白,具有化学性质稳定、产量高、成本低、特异性强且无任何毒副作用等特点;且由于动物种系发生距离远,不会发生交叉血清学反应。IgY在动物及人的疾病防制中具有显著优势。作者对IgY的结构特点、理化性质、作用机理及其在各类动物疾病防制方面的应用及发展前景进行了综述,尤其是在动物疾病的治疗方面,如治疗畜禽腹泻及烈性、急性、危害性大的病毒疫病;治疗人类疾病特别是严重危害人类财产安全的人畜共患病;其他方面如水产、皮毛动物等重大疾病。IgY在有效治疗动物疫病的同时,也减少了人畜共患病的可能,从人身安全方面考虑,也必将具有重大发展前景。     

纳米抗体在家畜传染病防治中应用的研究进展

随着社会经济水平的提高,人们对动物性食品的需求量显著扩大,对其安全性的要求也越来越严格。因此,家畜疾病的科学防控就显得尤为重要。目前,我国家畜疾病尤其是传染病的防控效果并不理想,仍需要不断开发和探索新的防控产品和防控策略。纳米抗体是最小的功能性抗原结合片段,和传统抗体相比,其具有分子量小、结构简单、稳定性高、易于基因改造、抗原特异性好、组织穿透力强等优点。纳米抗体源于骆驼科动物,作为一种新型抗体,在动物疾病防治技术中的研究和应用正逐步增加。本文重点综述纳米抗体在家畜传染病中的应用和研究进展,并结合发展趋势预测未来的研究重点。     

几种兽用合成抗菌药基于抗体快速检测技术研究进展

合成抗菌药在预防、治疗动物疾病等方面应用十分广泛,但不合理使用造成的耐药及残留问题日益严重,因此对兽用合成抗菌药的残留检测势在必行,而基于抗体的快速检测技术具有简便、灵敏度高、成本低等优点,发展前景十分广阔。作者综述了近年来针对磺胺类、氟喹诺酮类、喹噁啉类、硝基呋喃类、硝基咪唑类5大类兽用合成抗菌药物的抗体检测技术最新研究趋势,旨在促进残留快速检测技术的发展。     

Research Progress on Antibodies Rapid Detection Technology for Veterinary Synthetic Antibacterial Agents

Synthetic antibacterial agents are widely used in prevention and treatment of animal diseases. However, the irrational drugs use has caused severe drug residues issues and bacteria resistance.Therefore,it is particularly important to develop the rapid detection technology.The antibodies rapid detection technology has broad prospects because of its high sensitivity, simplicity, and low cost. The author reviewed the latest research on antibodies preparation and rapid detection methods for sulfonamides,fluoroquinolones, quinoxalines, nitrofurans and nitroimidazoles. It is great significant for the development of antibody rapid detection methods on the veterinary drug residues.     

Progress in the discovery and definition of monoclonal antibodies for use in feline research

The practice of veterinary medicine and research into both animal diseases and animal models of human disease are restricted by the scarcity of monoclonal antibodies (mAb) that react with animal proteins. One way to enlarge the repertoire of mAb to animal leukocyte differentiation antigens (LDA) is to test mAb specific to other species for cross-reactivity to the species of interest. We have tested a panel of 380 commercially available anti-human mAb for cross-reactivity to feline LDA. Twenty-six of these mAb cross-react with cat LDA and 19 others are of questionable cross-reactivity. Definition of mAb specificity in the cat is being investigated by multi-color flow cytometry (FCM) to compare test mAb specificity with that of mAb to known feline LDA. The addition of these cross-reactive mAb to the anti-feline mAb currently available will enhance studies in comparative medicine.     

Competitive ELISA for the detection of antibodies to Rift Valley fever virus in goats and cattle

Rift Valley fever virus (RVFV) is one of the important emerging viral diseases of serious impact in public health and animal hygiene both in human and animal industries. In this study, we developed a monoclonal antibody-based competitive ELISA for the detection of antibodies to RVFV in goats and cattle. The recombinant N protein of RVFV was expressed in E. coli with a six-histidine tag, and the purified N protein was used for detecting antigen with a competitive monoclonal antibody against RVFV antibodies. The competitive ELISA (C-ELISA) could detect antibodies at 9-11 days after inoculation in goats and cattle with a sensitivity of 94.7% (virus neutralization titer >32) and specificity of 99.7%, respectively. In addition, the C-ELISA did not show any cross-reactivity with positive sera against arboviruses such as Akabane, Aino, Chuzan, Ibaraki and bovine ephemeral fever virus, which are prevalent viral agents in ruminant animals throughout Southeast Asia. The results of the present study indicate that the C-ELISA is a simple, rapid and convenient serodiagnostic method for RVFV in goats and cattle.     

Seroprevalence of brucellosis, tularemia, and yersiniosis in wild boars (Sus scrofa) from north-eastern Germany

Brucellosis and tularemia are classical zoonotic diseases transmitted from an animal reservoir to humans. Both, wildlife and domestic animals, contribute to the spreading of these zoonoses. The surveillance of the animal health status is strictly regulated for domestic animals, whereas systematic disease monitoring in wildlife does not exist. The aim of the present study was to provide data on the prevalence of anti-Brucella, anti-Francisella and anti-Yersinia antibodies in wild boars from North-Eastern Germany to assess public health risks. A total of 763 sera of wild boars from Mecklenburg-Western Pomerania hunted in 1995/1996 were tested using a commercially available Brucella suis ELISA, an in-house lipopolysaccharide (LPS)-based Francisella ELISA, and commercially available Western blot kits for the detection of anti-Francisella and anti-Yersinia antibodies. The Yersinia enterocolitica O:9 LPS is able to induce serological cross-reactions indistinguishable from brucellosis due to a similar immunodominant epitope in the Brucella O-polysaccharide. The Yersinia Western blot assay was, therefore, based on five recombinant Yersinia outer proteins which have been proved to be specific for the serodiagnosis of yersiniosis. Anti-Brucella, anti-Francisella and anti-Yersinia antibodies were detected in 22.0%, 3.1%, and 62.6% of the wild boars, respectively. The high seroprevalence of tularemia and brucellosis in wild boars indicates that natural foci of these zoonoses are present in wildlife in Germany. However, the impact of transmission of zoonotic pathogens from wildlife to livestock is unknown. Only careful and systematic monitoring will help to prevent the (re)emergence of these zoonotic diseases in domestic animals and consequently human infection.     

Optimization of immunohistochemical methods using two different antigen retrieval methods on formalin-fixed paraffin-embedded tissues: experience with 63 markers.

Formalin fixation produces cross-links between the proteins and the fixative that alter the ability of some antibodies to recognize antigens. We used formalin-fixed, paraffin-embedded tissues to compare two different antigen retrieval methods for 63 antibodies used in the diagnosis of infectious and neoplastic diseases of animal species. Eighty-four percent of the antibodies needed some type of antigen retrieval for optimal results. Of those antibodies, 67.7% were monoclonal and 32.3% were polyclonal. Steam heat was the method of choice for 31 antibodies. Ten antibodies reacted only with steam heat, but 9 antibodies did not react when steam heat was used. Optimal results were obtained with enzyme digestion for 22 antibodies. Only 10 antibodies yielded optimal results without antigen retrieval; 64% of these antibodies were polyclonal. All antibodies against cytokeratins were optimally retrieved with proteinese K. Antigen retrieval appears to be necessary for the majority of antibodies when used with formalin-fixed, paraffin-embedded tissues.