Effects of co-inoculation of Bradyrhizobium elkanii BLY3-8 and Streptomyces griseoflavus P4 on Rj4 soybean varieties

Co-inoculation of selected nitrogen-fixing bacteria with plant growth-promoting bacteria is the promising way for the improvement of soybean production through enhancing plant growth, nodulation, and N₂ fixation. Therefore, this experiment was conducted to study the effects of co-inoculation of Bradyrhizobium elkanii BLY3-8 with Streptomyces griseoflavus P4 on plant growth, nodulation, N₂ fixation, N uptake, and seed yield of Rj₄ soybean varieties. Two experiments with completely randomized design and three replicates were done in this study. N₂-fixation ability of soybean was evaluated by acetylene reduction activity (ARA) and relative ureide method. In the first experiment, synergetic effect in N₂ fixation and nodulation was occurred in co-inoculation treatment (BLY3-8 + P4) in Yezin-3 and Fukuyutaka. Based on these results, co-inoculation effect of BLY3-8 and P4 was assessed on Yezin-3 and Fukuyutaka varieties at three different growth stages, using Futsukaichi soil under natural environmental conditions. This study shows that co-inoculation of BLY3-8 and P4 significantly increased N₂ fixation at V6 stage; plant growth, nodulation, N₂ fixation, and N uptake at R3.5 stage; and shoot growth, N uptake, and seed yield at R8 stage, in Rj₄ soybean varieties compared with the control. Significant difference in plant growth, nodulation, N₂ fixation, N uptake, and yield between co-inoculation and control, not between single inoculation and control, suggests that there is a synergetic effect due to co-inoculation of BLY3-8 and P4.     

Evaluation of effective Myanmar Bradyrhizobium strains isolated from Myanmar soybean and effects of coinoculation with Streptomyces griseoflavus P4 for nitrogen fixation

In order to substitute the use of chemical fertilizers in legume production, there is a need for the production of rhizobial inoculants which are capable of being used as biofertilizers. To achieve this, an effective symbiotic nitrogen (N) fixation between legumes and root nodule bacteria will be essential. Evaluation of effective Myanmar Bradyrhizobium (Jordan 1982) strains isolated from Myanmar soybean (Glycine max L. Merr.) and effects of coinoculation with Streptomyces griseoflavus Krainsky 1914 P4 for N fixation were studied in pot experiments using sterilized vermiculite and Hoagland solution in the Phytotron (25°C and 70% relative humidity) with completely randomized design and three replicates. N fixation ability of soybean was evaluated by acetylene reduction activity (ARA) by gas chromatography. It was found that MAS23 showed a relatively high degree of stability and a high level of ARA per plant on both Yezin-3 and Yezin-6 soybean varieties. In the symbiotic relationship between Bradyrhizobium strains and P4 experiments, the treatments consisted of six Bradyrhizobium strains (MAS23, MAS33, MAS34, MAS43, MAS48 and USDA110) and Streptomyces griseoflavus P4 were evaluated with four Myanmar soybean varieties (Yezin-3, Yezin-6, Hinthada and Shan Sein). In the Yezin-3 soybean variety, the best treatment for ARA per plant was found in the dual inoculation of P4 and MAS34. In the Yezin-6 soybean variety, the highest nodule dry weight was found in dual inoculation of P4 with MAS34 but the highest ARA per plant was observed in the dual inoculation of P4 and MAS23. On the other hand, single inoculation of MAS43 and coinoculation of P4 with MAS48 were significantly higher in N fixation of Hinthada, and coinoculation of P4 with MAS33 was significant improvement of ARA per plant (P < 0.05) in Shan Sein soybean.     

壳聚糖对NaCl胁迫下菜用大豆结瘤固氮的影响

【目的】研究壳聚糖对盐胁迫抑制菜用大豆结瘤固氮的缓解效应,为进一步探讨壳聚糖抗逆机理提供新的线索。【方法】以蛭石为基质,以菜用大豆‘特早王’–根瘤菌共生体系为研究对象,采用人工气候箱培养,研究NaCl胁迫下壳聚糖对菜用大豆根瘤形成、生物固氮的影响。菌种为与‘特早王’共生匹配性较好的快生根瘤菌N18。接种后的植株进行如下4个处理:1)叶面喷施清水,根部浇灌无氮营养液(CK);2)叶面喷施壳聚糖水溶液,根部浇灌无氮营养液(CTS);3)叶面喷施清水,根部浇灌溶有NaCl的无氮营养液(Cl);4)叶面喷施壳聚糖水溶液,根部浇灌溶有NaCl的无氮营养液(CTS+Cl)。上述各处理施用的水或水溶液均为无菌水配制,NaCl处理的浓度为50 mol/L,CTS处理的适宜浓度为200 mg/L。接种30天后,将大豆植株取出,用清水将根部蛭石冲洗干净后,立即测定根瘤固氮酶活性、根瘤数及根瘤鲜重,然后测定根瘤豆血红蛋白含量和根系活力,最后测植株干重和全氮量。【结果】氯化钠胁迫下,植株干重显著下降,与CK相比降幅达49%,喷施壳聚糖后(CTS+Cl),降低幅度显著减小,但依然显著低于CK (P <0.05)。无盐条件下,与CK相比,壳聚糖处理(CTS)增加植株干重的效果不明显。喷施壳聚糖显著增加了菜用大豆的根瘤数、根瘤鲜重、植株含氮量、根系活力、豆血红蛋白含量及固氮酶活性(P <0.05)。NaCl胁迫显著抑制了菜用大豆的结瘤固氮作用,其中根瘤数、根瘤鲜重分别较CK下降了79%、90%,而壳聚糖处理(CTS+Cl)使菜用大豆在盐逆境下的结瘤数、根瘤鲜重、植株全氮含量、根系活力、豆血红蛋白含量及固氮酶活性等均显著回升,增幅分别达对照的29%、20%、17%、48%、19%、21%,但均显著低于CK。【结论】非NaCl胁迫下,喷施壳聚糖可以显著促进菜用大豆结瘤,提高豆血红蛋白含量及固氮酶活性,最终增加植株含氮量。在NaCl胁迫下,外源壳聚糖可以显著缓解氯化钠胁迫导致的对根系活力和结瘤固氮的影响。因此,叶面喷施壳聚糖是促进菜用大豆结瘤固氮和生长的有效措施。     

Interactive effects of co-inoculation of Bradyrhizobium japonicum strains and mycorrhiza species on soybean growth and nutrient contents in plant

The main objective of this study was to investigate the effects of co-inoculation with different strains of Bradyrhizobium japonicum (i.e. Helinitro, Rizoking, and Nitragin) and arbuscular mycorrhizal fungi (AMF) species (i.e. Glomus fasciculatum, Glomus versiforme, Glomus intraradices, Glomus mosseae, and Glomus etunicatum) on soybean growth, fungal root colonization, and nutrient uptake of nitrogen (N), phosphorus (P), zinc (Zn), iron (Fe), and copper (Cu). Co-inoculation with various AMF species and rhizobia significantly (p<0.01) increased the soybean biomass production as compared to the non-inoculated controls. Furthermore, AMF colonization of roots of soybean plants increased by 79, 70.1, 67, 63, 57.5, and 50.1% in the presence of G. fasciculatum (GF), G. versiforme (GV), G. intraradices (GI), G. mosseae (GM), and G. etunicatum (GE), and Gmix (a mixed culture of fungi), respectively. Higher nutrient contents were observed in plants co-inoculated with Helinitro and GF. More insight into these results will enable optimization of the effective use of AM fungi in combination with their bacterial partners as a tool for increasing soybean yields in Iran; however, its general analytical framework could be applied to other parts of the world.     

玉米/大豆、玉米/花生间作对作物氮素吸收及结瘤固氮的影响

禾本科与豆科作物间作具有显著的增氮作用。为探明玉米/大豆、玉米/花生间作模式的氮素吸收、氮营养竞争能力及豆科结瘤特性的变化,解释玉米与豆科间作体系的增氮效应,通过田间试验,设置玉米单作（MM）、大豆单作（SS）、玉米/大豆间作（MS）、花生单作（PP）、玉米/花生间作（MP）等5种种植模式,研究不同种植模式对作物氮素积累、氮营养竞争强弱及豆科结瘤固氮特性的调控作用。结果表明,与单作相比,间作显著降低玉米和大豆的氮素积累量,对花生的氮素积累量影响不显著。5种模式系统氮素积累总量表现为MS > SS > MP,PP和MM处理最低且差异不显著,MS处理比MP处理显著高21.8%。与MM处理相比,MS和MP处理的玉米氮素积累量分别降低20.5%和11.7%,其中MP处理籽粒、叶片和茎秆氮素积累量比MS处理高8.9%、21.2%和14.3%。与SS处理相比,MS处理的大豆氮素积累量降低28.5%,其中,中行、边行分别降低10.1%、15.4%。玉米相对大豆氮营养竞争比率表现为强（CR_ms>1）,相对花生则表现为弱（CR_mp<1）。与SS处理相比,五叶期MS处理的大豆根瘤数量显著增加,根瘤鲜重无显著差异,盛花期后根瘤数量和鲜重均显著降低;MS处理的大豆根瘤固氮酶活性均降低,且中行降低幅度更大。与PP处理相比,开花期MP处理的花生根瘤数量和鲜重均显著增加,下针期后均显著降低;MP处理的花生根瘤固氮酶活性均降低,且边行降低幅度更大。各间作模式作物的氮素积累量虽然降低,但间作模式的系统氮素积累量却显著高于各单作模式,两种间作模式中MS处理的氮素积累总量最高。     

不同施氮量对甜瓜养分吸收、分配、利用及产量的影响

通过田间膜下滴灌栽培,研究不同氮素水平对甜瓜养分吸收、 分配及产量的影响。结果表明,增施氮肥显著提高了甜瓜的氮、 磷、 钾的积累量,特别是显著提高了甜瓜后期氮、 磷、 钾的积累量。在施P2O5 140 kg/hm2、 K2O 150 kg/hm2 的基础上施N 225 kg/hm2,甜瓜的养分吸收量和产量均为最高,各施氮处理甜瓜氮肥利用率在11%~29%之间,且随施氮量的增加而降低。甜瓜对钾的吸收量最高,氮次之,磷最少,表明甜瓜是喜钾作物。     

Effects of changes in applied nitrogen concentrations on nodulation,nitrogen fixation and nitrogen accumulation during the soybean growth period

ABSTRACT

The specific mechanism by which nitrogen application affects nodulation and nitrogen fixation in legume crops remains uncertain. To further study the effects of nitrogen application on soybean nodulation and nitrogen accumulation, three consecutive tests were performed during the VC-V4, V4-R1 (10 days), and R1-R2 (10 days) growth periods of soybean. In a dual-root soybean system, seedlings on one side were watered with a nutrient solution containing NH₄⁺ or NO₃^? as the N source (N+ side), and those on the other side were watered with a nitrogen-free nutrient solution (N- side). During the VC-R2 period, on the N+ side, high nitrogen treatment inhibited nodule growth and nitrogenase activity (EC 1.18.6.1), and the inhibition was significantly increased with increasing high nitrogen supply time (10 days, 20 days). When the high nitrogen treatment time reached 20 days, the specific nitrogenase activity (C₂H₄ μmol^?1 g^?1 nodule dry mass h^?1) was similar to that in the low nitrogen treatment, indicating that the nitrogen fixation capacity per gram of dry mass nodules was almost the same. Therefore, it is assumed that long-term high nitrogen treatment mainly reduces nitrogen fixation by reducing the nodule number. The effect of nitrogen concentration on the roots on the N+ side was greater than that on the N- side. Taken together, these results indicate that nitrogen application affects a contact-dependent local inhibition of root nodule growth, nitrogenase activity, and nitrogen accumulation. The whole plant systematically regulates specific nitrogenase activity, and high nitrogen inhibition is recoverable.     

东北黑土区大豆生长、结瘤及产量对氮、磷的响应

氮肥和磷肥显著影响大豆的结瘤和产量。然而在土壤肥力较高、速效养分有效性差的东北地区,有关氮肥和磷肥施用量对大豆结瘤和产量影响的研究较少。本试验采用裂区田间试验,设置3个氮（N）水平（0、20 和 50 kg/hm2）和 3 个磷（P）水平（0、 20 和 40 kg/hm2）,研究氮、 磷及其交互作用对大豆生长发育、 结瘤特征及产量的影响。结果表明, 单施氮肥大豆生物量和产量随着施氮量的增加而增加,而根瘤数量、 干重、 大小和结瘤指数呈逐渐下降的趋势。单施磷肥促进大豆生物量、 产量、 根瘤数量、 干重、 大小和结瘤指数的增加,但其增幅低于施氮处理下的增幅。氮磷对大豆生长和产量促进作用高于单施氮和单施磷处理,但差异不显著;氮磷处理下的根瘤数量、 干重、 大小和结瘤指数低于单施磷处理;氮磷处理下N2（N 50 kg/hm2）处理下的大豆根瘤数量、 干重、 大小和结瘤指数高于N1处理（N 20 kg/hm2）下的,随着施磷量的增加大豆根瘤数量、 干重、 大小和结瘤指数增加,施磷能够抵消氮对大豆根瘤产生和形成的抑制。氮、 磷及其交互作用对大豆根瘤的影响都是直接的,并且不是通过促进大豆生长间接促进的。因此氮和磷均是限制东北地区大豆结瘤和产量的因素,但氮是主导因素。若要获得大豆高产,氮肥施用量需要控制在50 kg/hm2,磷肥在40 kg/hm2;但若想最大的发挥大豆的结瘤固氮功能,那么应该不施或者减少氮肥的施用量到20 kg/hm2,磷肥仍在40 kg/hm2。     

有机肥氮替代部分化肥氮对棉花养分吸收、氮素利用和产量的影响

为探讨有机肥氮替代化肥氮在新疆棉花生产中的增产增效作用,在滴灌条件下,采用连续2年定位试验,研究了不施氮(CK)、牛粪堆肥(OM)、农民常规施肥(CF)和不同有机肥氮替代10%(OF1)、20%(OF2)和30%(OF3)的化肥氮时,对棉花氮磷钾养分吸收、氮素利用率及产量的影响。与单施化肥相比,有机肥氮替代部分化肥氮均有利于棉花氮磷钾养分吸收,可提高棉花氮素表观利用率、偏生产力、肥料氮贡献率和农学效率。在有机肥氮替代10%化肥氮的情况下,氮素利用率最高并能提高氮素表观利用率10.5个百分点,棉花产量增加6.8%(P<0.05),而且棉花经济效益与单施化肥相当。有机肥氮替代20%的化肥氮时,棉花产量增加7.9%(P<0.05)。有机肥氮替代30%化肥氮,获得与单施化肥相当的产量,氮素表观利用率仅提高3.4个百分点。综合养分吸收、氮素利用效率、产量及经济效益等方面考虑,有机肥氮替代10%化肥氮是该地区中等肥力棉田增产稳产、氮肥增效的合理施肥方式。     

氮肥运筹对夏玉米产量、品质及植株养分含量的影响

通过田间试验研究了不同氮肥运筹方式对夏玉米产量、 品质及植株养分含量的影响。结果表明,在氮肥施用量较低时,玉米籽粒产量及粗蛋白、 粗淀粉和粗脂肪等营养品质指标随氮肥用量的增加而提高; 当氮肥用量达到一定数量之后,则不随氮肥用量的增大而增加,甚至有所降低。在氮肥施用量为 N 300 kg/hm2,基肥∶拔节肥∶大喇叭口期追肥比例为5∶0∶5（A2）时玉米籽粒产量最高为10902 kg/hm2,比不施肥（对照）增产140.5%,比相同施氮量条件下其他基追比处理增产4.6%~12.3%。随着施氮量增加玉米籽粒氮、 磷、 钾含量呈先增后减趋势; 增施氮肥能显著提高夏玉米籽粒粗蛋白含量,在相同施氮量条件下,玉米籽粒氮素和粗蛋白含量在A2运筹方式下最高,说明该氮肥运筹方式能改善玉米籽粒的品质。     

Effects of root exudates of Rj2Rj3- and Rj4-genotype soybeans on growth and chemotaxis of Bradyrhizobium japonicum

Almost all of the soybean cultivars (Glycine max L. Merr.) form nodules on their roots by infection with Bradyrhizobium japonicum. However, it has been observed that the soybean cultivars harboring nodulation conditioning genes, for instance Rj ₂, Rj ₃, and Rj ₄, do not form effective nodules with some strains of B. japonicum. Ishizuka et al. (1991b) classified the rhizobia isolated from field-grown soybeans into nodulation-types A, B, and C based on the compatibility with these Rj-soybean cultivars. Nodulation-type B is incompatible with Rj ₂ Rj ₃-cultivars and type C is incompatible with Rj ₄-cultivars. Nodulation-type A rhizobia are compatible with both Rj ₂ Rj ₃ - and Rj ₄-cultivars. Furthermore, the Rj-cultivars were found to prefer appropriate nodulation-type rhizobia for nodulation, that is, non-Rj-, Rj ₂ Rj ₃ -, and Rj ₄-cultivars prefer nodulation-types A, C, and B rhizobia, respectively (Ishizuka et al. 1991a). In the present study, in order to examine the mechanisms of preference of Rj-cultivars for rhizobial strains for nodulation, root exudates from Rj-cultivars were examined for their effects on the growth rate and chemotaxis of various types of B. japonicum strains.     

Effect of organic matter on the growth,nodulation, and nitrogen fixation of soybean grown under acid and saline conditions

Abstract

A field experiment was conducted to examine the effects of two organic materials (Bark and Tenporon composts) on the growth, nodulation and nitrogen fixation (measured as acetylene-reducing activity) of soybean plants (Glycine max L.) under acid and saline conditions. These organic materials were incorporated into acid and saline (induced by irrigating the soil with a NaCl solution) soils at 4% rate of application.

These composts tended to improve nodule formation in soybean under acid and saline conditions, especially in the plants treated with Bark compost. Acetylene-reducing activity was significantly (P<0.05) increased by these composts under acid condition.

These composts also tended to improve shoot growth under acid and saline conditions, presumably due to the improvement of the soil moisture status of the soils and nutrient uptake. These results suggest that the growth of soybean could be improved by the application of organic matter under acid and saline conditions.     

Influence of calcium carbide formulations on growth,yield and nitrogen uptake of wheat under field conditions

Field experiments were conducted in 2006–2007 and 2007–2008 to evaluate the effects of three calcium carbide (CaC₂) based formulations (slow release sources of acetylene and ethylene) - matrix-1 [(M-1)(21% CaC₂, 58% polyethylene and 21% plaster of paris)], matrix-2 [(M-2)(42% CaC₂, 48% polyethylene and 10% plaster of paris)] and paint coated [(PC)(35% CaC₂, 44% paint and 21% plaster of paris)] - on nitrification inhibition; growth, yield and nitrogen (N)-uptake of wheat. All the three CaC₂ formulations (CCF,s) inhibited nitrification, reduced plant height, and improved N-uptake, spike bearing tillers, 1000-grains weight and yield of Inqulab-91 and Bhakhar-02 wheat cultivars. Increase in grain yield was found maximum (22% over the control) with M-1 applied at 30 kg CaC₂ ha^?1; followed by M-2 and PC. Furthermore, CaC₂ significantly reduced lodging in Bhakhar-02 plots. It is concluded that grain yield of wheat can be enhanced by the application of CaC₂ formulations particularly M-1 along with NPK fertilizers.     

种植密度和施氮量对不同株高夏玉米产量和氮素利用的影响

【目的】 本研究旨在探讨不同株高夏玉米产量和氮素利用对种植密度和施氮量的响应。 【方法】 以矮秆玉米品种登海 661 (DH661)、中秆品种郑单 958 (ZD958) 和高秆品种鲁单 981 (LD981) 为试验材料,在大田条件下设置 2 个种植密度 (67500 和 82500 plant/hm2) 和 3 个施氮量 (N 0、180 和 270 kg/hm2),以不施氮为对照,研究种植密度和施氮量对不同株高夏玉米氮素吸收与利用特性的影响。 【结果】 在密度为 82500 plant/hm2 条件下,品种 DH661、ZD958 和 LD981 的籽粒产量分别较 67500 plant/hm2 分别提高 5.0%、10.2% 和 12.5%;施氮 180 和 270 kg/hm2 处理 DH661、ZD958 和 LD981 的籽粒产量差异不显著。高密度条件下 (82500 plant/hm2),施氮 270 kg/hm2 时 DH661 氮素转运效率和转运氮贡献率较 180 kg/hm2 显著降低,ZD958 和 LD981 变化不显著;低密度条件下 (67500 plant/hm2),施氮 270 kg/hm2 时 DH661 和 ZD958 氮素转运效率和氮素转运贡献率较 180 kg/hm2 显著提高,LD981 的则显著降低。DH661 的氮素利用效率较 ZD958 和 LD981 分别提高 7.4% 和 39.1%,LD981 的氮素吸收效率较 ZD958 和 DH661 品种分别提高 18.9% 和 25.0%。 【结论】 在低密度 67500 plant/hm2 条件下,增施氮肥,矮秆和中秆品种的氮素转运效率和氮素转运贡献率显著降低,而高秆品种的则提高。高密度 82500 plant/hm2 条件下,增施氮肥,矮秆品种氮素转运效率和氮素转运贡献率显著降低,中秆和高秆品种的无显著变化。      

氮素运筹对玉米干物质积累、氮素吸收分配及产量的影响

田间小区试验,研究了氮素运筹对玉米干物质累积、氮素吸收分配及产量的影响。结果表明,施氮和有机肥可以延长干物质积累的旺盛时期,使玉米干物质总量积累速率最大的时刻推后1～3d,增加了玉米的干物质积累量。氮肥配施有机肥能延长氮素积累的旺盛时期,其中,N2+M处理氮素积累的旺盛时期△t分别比N0、N1、N2、N3和FP处理延长了6、4、6、1和7d,该时段吸N量比FP处理增加 0.25 g/plant,比等氮量的N2处理增加0.24 g/plant。播前施有机肥 30 t/hm2,在减 N 26.83%的情况下,玉米吸氮量比常规施肥（FP）提高 6.52%,氮素利用率达54.31%,高于常规施肥的 33.27%;玉米增产24.12%,比常规施肥增收2696元/hm2。     

Enhanced soybean (Glycine max L.) plant growth and nodulation by Bradyrhizobium japonicum-SB1 in presence of Bacillus thuringiensis-KR1

Abstract

Nodulation and subsequent nitrogen fixation are important factors that determine the productivity of soybean (Glycine max L.). The beneficial effects of nodulation can be enhanced when rhizobial inoculation is combined with plant-growth-promoting bacteria (PGPB). The PGPB strain Bacillus thuringiensis-KR1, originally isolated from the nodules of Kudzu vine (Pueraria thunbergiana), was found to promote growth of soybean plants (variety VL Soya 2) under Jensen's tube and growth pouch conditions, when co-inoculated with Bradyrhizobium japonicum-SB1. Co-inoculation with Bacillus thuringiensis-KR1 (at a cell density of 10 cfu) provided the highest and most consistent increase in nodule number, shoot weight, root weight, root volume, and total biomass, over rhizobial inoculation and control, under both conditions. The results demonstrate the potential benefits of using nonrhizobial nodule occupants of wild legumes for the co-inoculation of soybean, with Bradyrhizobium japonicum-SB1, in order to achieve plant-growth promotion and increased nodulation.     

Effects of Rhizobium and vesicular-arbuscular mycorrhiza inoculations on nodulation,symbiotic nitrogen fixation and soybean yield in subtropical-tropical fields

Summary Field experiments were carried out to determine the effects of single and mixed inoculations with Rhizobium and vesicular-arbuscular mycorrhiza (VAM) on nodulation, symbiotic N₂ fixation and yield of soybeans in six Taiwan subtropical-tropical sites. Inoculation with Rhizobium alone significantly increased nodulation, nodule weight and nitrogenase activity of nodules in three out of six experimental fields, and affected soybean yields in the range –13% to + 134%. Inoculation with VAM fungi alone did not have a significant effect on nodulation and nitrogenase activity. Mycorrhiza inoculation affected soybean yields in the range –13% to + 65%, but only the yield increases at one out of six sites with N application were statistically significant. Mixed inoculation with Rhizobium and mycorrhiza affected yields in the range –8% to + 145% A synergistic effect from mixed inoculation of Rhizobium-mycorrhiza on soybean yields was found in one out of six experimental fields. The yield response to N application (40 kg N ha^–1) in these six paddy-field trials was not significant. These results suggest that single or mixed inoculation of rhizobia can greatly assist soybean grain yields and can replace N fertilizers.     

氯化钠不同浓度对夏玉米生长和吸氮的影响

采用珍珠岩为基质的温室盆栽试验,研究不同盐分(NaCl)水平对夏玉米生长和吸氮的影响。试验设灌溉水中NaCl浓度为0、10、20、40和60mmol.L5个处理。结果表明,在出苗后的25～101d(7月25日～10月10日),当NaCl浓度达到60mmol.L时,明显降低了夏玉米的叶面积、株高、干物质量和根长;盐分对植株体内各器官的氮含量影响不大。随着NaCl含量的增加,植株的总氮累积量将减少,且植株中累积氮量随时间呈二次函数增长。     

Root growth,nutrient uptake and yield of soybean cultivars grown in the field

Abstract

Soybean (Glycine max L. Merr.) cultivars differ in their root morphology and their nutrient uptake capabilities. The relation between root growth, P and K uptake, and grain yield was investigated using eight cultivars grown in the field on Raub (Aquic Argiudoll) silt loam which received 49 kg P/ha and 93 kg K/ha. Hobbit (maturity group III, determinate) was among the highest in grain yield, P and K uptakes, and root system length. However, this cultivar was intermediate in its relative efficiency to utilize P and K to produce grain yield; among the most efficient cultivars were Asgrow 3127 (maturity group II) and Williams‐79 (maturity group III). The hay cultivar, Wilson‐6, was the least efficient. It was concluded that even though grain yield was correlated with nutrient uptake, selection for higher yields was not necessarily a selection for higher efficiency in utilization of fertilizer for grain production.     

密度和施氮量对油菜生长发育及产量的影响

为探讨密度和施氮量互作效应对油菜生长发育及产量的影响,以甘蓝型油菜品种华油杂9号为材料,在施氮120、240和360 kg·hm~(-2)的条件下,分别设密度15万、30万和45万株·hm~(-2)处理,分析了施氮量与密度对油菜生长发育动态、产量构成因素和产量的影响,及三者之间的相关关系。结果表明,油菜生育期随施氮量增加略呈延长趋势,而与密度无显著相关。株高随密度增大而下降,随施氮量增加表现出先升后降趋势。在油菜整个生育期中,随着施氮量和密度的增加,单位面积群体根颈粗、绿叶数、功能叶叶绿素、干物质积累量、角果分配比例、有效分枝数、有效角果数大体均显著增大,并与油菜产量呈显著正相关,且密度的影响总体大于氮肥的影响。随着施氮量和密度的增加,各相邻处理间差异总体呈逐渐变小趋势。120 kg·hm~(-2)施氮量和45万株·hm~(-2)种植密度组合不仅可以达到360 kg·hm~(-2)施氮量和15万株·hm~(-2)种植密度组合的产量,同时每公顷可以节省120 kg的氮肥投入,在实际生产中适量减少氮肥并适度提高密度依然可以获得高产。