共查询到20条相似文献,搜索用时 15 毫秒
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Site-specific recombination between homologous chromosomes in Drosophila 总被引:22,自引:0,他引:22
K G Golic 《Science (New York, N.Y.)》1991,252(5008):958-961
The ability to mark a cell and its descendants genetically so that the resulting cell clone can be distinguished from neighboring cells facilitates studies in animal biology and development. A method of generating clones by inducing homologous mitotic recombination in Drosophila with a site-specific yeast recombinase is described. This method allows for frequent mosaicism after mitotic exchange is induced at predefined sites in the genome. 相似文献
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Gene encoding the beta subunit of S100 protein is on chromosome 21: implications for Down syndrome 总被引:17,自引:0,他引:17
R Allore D O'Hanlon R Price K Neilson H F Willard D R Cox A Marks R J Dunn 《Science (New York, N.Y.)》1988,239(4845):1311-1313
S100 protein is a calcium-binding protein found predominantly in the vertebrate nervous system. Genomic and complementary DNA probes were used in conjunction with a panel of rodent-human somatic cell hybrids to assign the gene for the beta subunit of S100 protein to the distal half of the long arm of human chromosome 21. This gene was identified as a candidate sequence which, when expressed in the trisomic state, may underlie the neurologic disturbances in Down syndrome. 相似文献
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The "Down syndrome critical region" (DSCR) is a chromosome 21 segment purported to contain genes responsible for many features of Down syndrome (DS), including craniofacial dysmorphology. We used chromosome engineering to create mice that were trisomic or monosomic for only the mouse chromosome segment orthologous to the DSCR and assessed dysmorphologies of the craniofacial skeleton that show direct parallels with DS in mice with a larger segmental trisomy. The DSCR genes were not sufficient and were largely not necessary to produce the facial phenotype. These results refute specific predictions of the prevailing hypothesis of gene action in DS. 相似文献
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O'Doherty A Ruf S Mulligan C Hildreth V Errington ML Cooke S Sesay A Modino S Vanes L Hernandez D Linehan JM Sharpe PT Brandner S Bliss TV Henderson DJ Nizetic D Tybulewicz VL Fisher EM 《Science (New York, N.Y.)》2005,309(5743):2033-2037
Aneuploidies are common chromosomal defects that result in growth and developmental deficits and high levels of lethality in humans. To gain insight into the biology of aneuploidies, we manipulated mouse embryonic stem cells and generated a trans-species aneuploid mouse line that stably transmits a freely segregating, almost complete human chromosome 21 (Hsa21). This "transchromosomic" mouse line, Tc1, is a model of trisomy 21, which manifests as Down syndrome (DS) in humans, and has phenotypic alterations in behavior, synaptic plasticity, cerebellar neuronal number, heart development, and mandible size that relate to human DS. Transchromosomic mouse lines such as Tc1 may represent useful genetic tools for dissecting other human aneuploidies. 相似文献
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Beta amyloid gene duplication in Alzheimer's disease and karyotypically normal Down syndrome 总被引:12,自引:0,他引:12
J M Delabar D Goldgaber Y Lamour A Nicole J L Huret J de Grouchy P Brown D C Gajdusek P M Sinet 《Science (New York, N.Y.)》1987,235(4794):1390-1392
With the recently cloned complementary DNA probe, lambda Am4 for the chromosome 21 gene encoding brain amyloid polypeptide (beta amyloid protein) of Alzheimer's disease, leukocyte DNA from three patients with sporadic Alzheimer's disease and two patients with karyotypically normal Down syndrome was found to contain three copies of this gene. Because a small region of chromosome 21 containing the ets-2 gene is duplicated in patients with Alzheimer's disease, as well as in karyotypically normal Down syndrome, duplication of a subsection of the critical segment of chromosome 21 that is duplicated in Down syndrome may be the genetic defect in Alzheimer's disease. 相似文献
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Down to the wire for the NF gene 总被引:5,自引:0,他引:5
L Roberts 《Science (New York, N.Y.)》1990,249(4966):236-238
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Nonrandomness of translocations in man: preferential entry of chromosomes into 13-15-21 translocations 总被引:6,自引:0,他引:6
F Hecht M P Case E W Lovrien J V Higgins H C Thuline J Melnyk 《Science (New York, N.Y.)》1968,161(839):371-372
Lymphocytes from 20 individuals with Down's syndrome due to 13-15/21 centric-fusion translocations were studied by autoradiography after continuous late labeling with tritiated thymidine. In no case was chromosome 13 involved; chromosome 14 was involved in 18 cases, and chromosome 15 in two cases. These results are similar to those from 13 previously studied cases and indicate that the entry of chromosomes 13-15 into translocations is nonrandom. This nonrandomness is not a simple function of chromosome size or shape, since chromosomes 13-15 are acrocentrics of similar size. 相似文献
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SANDNES GC 《Science (New York, N.Y.)》1954,119(3094):508-509
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分蘖洋葱组织培养染色体数目变化 总被引:5,自引:0,他引:5
采用茎尖分生组织培养技术 ,获得分蘖洋葱无毒试管苗。通过染色体压片 ,对分蘖洋葱组培染色体数目变化进行了研究。结果表明 ,茎尖分生组织培养苗遗传稳定 ,其染色体未发生变异 ,均为 2 n=16 ;愈伤组织及其再生苗遗传稳定性差 ,愈伤组织染色体变异率为 33.85 % ,其中单倍体占 9.2 3% ,四倍体占 15 .39.,非整倍体占 9.2 3% ;愈伤组织分化苗染色体变异率为 2 4 .6 2 % ,其中四倍体占 16 .93% ,非整倍体占 7.6 9%。未发现三倍全的存在。 相似文献
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Although the voluntary intake of drugs of abuse is a behavior largely preserved throughout phylogeny, it is currently unclear whether pathological drug use ("addiction") can be observed in species other than humans. Here, we report that behaviors that resemble three of the essential diagnostic criteria for addiction appear over time in rats trained to self-administer cocaine. As in humans, this addiction-like behavior is present only in a small proportion of subjects using cocaine and is highly predictive of relapse after withdrawal. These findings provide a new basis for developing a true understanding and treatment of addiction. 相似文献
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Records of carbon and nitrogen isotopes in biogenic silica and carbon isotopes in planktonic foraminifera from deep-sea sediment cores from the Southern Ocean reveal that the primary production during the last glacial maximum was lower than Holocene productivity. These observations conflict with the hypothesis that the low atmospheric carbon dioxide concentrations were introduced by an increase in the efficiency of the high-latitude biological pump. Instead, different oceanic sectors may have had high glacial productivity, or alternative mechanisms that do not involve the biological pump must be considered as the primary cause of the low glacial atmospheric carbon dioxide concentrations. 相似文献