薏米蛋白的酶水解工艺优化

为确定蛋白酶水解薏米蛋白的最佳工艺条件,以氮溶解指数(NSI)和水解度(DH)为指标对中性蛋白酶、碱性蛋白酶、木瓜蛋白酶、胰蛋白酶进行筛选,确定碱性蛋白酶为最佳水解用酶,并通过单因素和二次回归正交旋转试验,建立了碱性蛋白酶水解薏米蛋白的数学模型。结果表明,碱性蛋白酶在底物质量浓度39.95 g/L,酶用量1 201.75 U/g,温度54.61℃,pH值7.99,反应时间4 h的条件下,水解后的氮溶解指数可达95.79%。     

欧李仁蛋白多肽的制备

以欧李仁蛋白为底物,采用碱性蛋白酶、中性蛋白酶和酸性蛋白酶进行分步复合酶解,以水解度为指标,确定其分步复合酶解的条件。结果表明,碱性蛋白酶的最适条件为底物质量分数5%,酶添加量为1.5%(基于底物蛋白质的质量),温度50℃,pH值10;中性蛋白酶添加量为5%,温度40℃,pH值7;酸性蛋白酶添加量5%,温度50℃,pH值5。分别水解30 min,经这3种酶酶解后其多肽质量浓度可达27.566 1 mg/mL。     

制备高得率豌豆多肽工艺参数的研究

采用木瓜蛋白酶、碱性蛋白酶、胰蛋白酶、风味蛋白酶和中性蛋白酶制备豌豆肽,并采用单因素试验方法进行了不同底物质量分数、水解时间、不同酶与底物质量比对豌豆肽的水解度及肽得率的影响,最终筛选出制备高得率豌豆肽的工艺参数为:碱性蛋白酶、底物质量分数7%,酶与底物质量比3%,水解3 h的水解产物具有较高的水解度和肽得率。     

碱性蛋白酶水解鸡血清蛋白制备ACE抑制肽的研究

采用碱性蛋白酶水解鸡血清蛋白制备ACE抑制肽。以ACE抑制率和水解度为评价指标,研究温度、p H值、底物质量分数、酶与底物浓度比、时间对水解效果的影响。通过单因素试验对碱性蛋白酶水解鸡血清蛋白制备ACE抑制肽的工艺条件进行优化。结果表明,最优水解条件为温度50℃,p H值8.0,底物质量分数6%,酶与底物浓度比6 000 U/g,时间4 h,此时ACE抑制率和水解度分别为63.07%±0.69%和17.22%±0.31%。     

碱性蛋白酶水解蚕蛹蛋白质工艺的研究

经5种蛋白酶比较试验的筛选,采用碱性蛋白酶进行水解蚕蛹蛋白的研究。在单因素试验的基础上,以水解度为考察指标,通过正交试验优化水解工艺条件,研究温度、底物浓度、时间、加酶量和pH值对蚕蛹蛋白质水解效果的影响。结果表明,碱性蛋白酶水解蚕蛹蛋白质的最优工艺条件为:处理温度55℃,底物浓度3%,时间6 h,加酶量3%,pH 9.5,蚕蛹蛋白的水解度可达22.91%左右。     

固定化酶制备大豆肽的研究

应用固定化碱性蛋白酶水解大豆分离蛋白,并对制备大豆肽的工艺条件进行正交试验,结果表明,应用固定化碱性蛋白酶制备大豆肽的最佳工艺条件的底物浓度为3.8mg/mL、温度60℃、pH值为8.8、时间为3h,制得的大豆肽的水解度为39.20%,可溶性蛋白溶解指数(NSI)为0.9957。     

响应面法优化燕窝不溶性蛋白酶解工艺的研究

研究旨在应用酶解技术将燕窝中不溶性蛋白转换为水溶性多肽,使分子量降低,有利于人体消化吸收,以期为燕窝新产品的开发奠定基础。以水解度和肽得率为指标,筛选出碱性蛋白酶对燕窝中不溶性蛋白进行酶解。通过单因素实验研究酶解时间、酶加量、底物浓度对燕窝多肽得率的影响,并利用响应曲面法对燕窝不溶性蛋白酶解工艺进行优化。结果表明：酶解的最佳工艺为：底物浓度为2.5%,酶加量为4%,酶解时间为3.6h,得到的肽得率为33.59%。通过实验对该模型进行验证：得到肽得率为33.38%,相对误差为0.56%,说明该模型能较好地反映出燕窝不溶性蛋白酶解情况。此外,通过凝胶电泳实验证明燕窝不溶性蛋白通过碱性蛋白酶酶解,分子量有效降低。该实验获得了燕窝多肽,为后续产品进一步深入开发和应用提供一定理论基础。     

酶水解乳清蛋白工艺条件的研究

采用4因素二次正交旋转实验,对蛋白酶A水解乳清蛋白的工艺条件(pH值、温度、酶与底物浓度比、水解时间)进行优化。建立了以乳清蛋白水解度为响应值的二次正交旋转回归模型,依据该模型确定以乳清蛋白水解度为响应值的最佳水解条件分别为:初始pH值为6.87,温度为59.6℃,酶与底物浓度比为2000.31 U/g,水解时间为2.91 h。     

固定化酶制备大豆肽的研究

应用固定化碱性蛋白酶水解大豆分离蛋白,并对制备大豆肽的工艺条件进行正交试验,结果表明,应用固定化碱性蛋白酶制备大豆肽的最佳工艺条件的底物浓度为3.8 mg/mL、温度60℃、pH值为8.8、时间为3 h,制得的大豆肽的水解度为39.20%,可溶性蛋白溶解指数(NSI)为0.995 7.     

固定化酶制备大豆肽的研究

应用固定化碱性蛋白酶水解大豆分离蛋白,并对制备大豆肽的工艺条件进行正交试验,结果表明,应用固定化碱性蛋白酶制备大豆肽的最佳工艺条件的底物浓度为3.8 mg/mL、温度60℃、pH值为8.8、时间为3 h,制得的大豆肽的水解度为39.20%,可溶性蛋白溶解指数（NSI）为0.995 7.     

冷冻干燥速溶蛋花汤的加工技术

介绍了运用冷冻干燥技术进行速溶蛋花汤的加工,研究了生产工艺、特点以及影响产品质量的主要因素。试验表明,采用冷冻干燥技术加工的速溶蛋花汤,具有营养丰富、风味独特、方便快捷等特点。     

Measuring of Quantity of Matter-mass and the Measuring of Inertia of Matter-momentclm

A new definition of inertia,i.e. the momentum is the measuring of inertia of matter, is proposed. The article through and etc., subjects, the correctness of the new notion of inertia:The monentum is the measuring of inertia has been demonstrated, and the unsolved contradictions of the traditional definition of inetia. And the mass is the measuring of inertia has been revealed.     

香蕉遗传转化方法研究进展

香蕉是热带亚热带发展中国家重要的粮食作物和碳水化合物来源。但近年来,香蕉生产受到严重的病虫危害。大多数香蕉栽培品种是三倍体,生长周期长,而且不孕。由于没有种子,给繁殖和育种带来一定的困难。遗传转化技术的发展为香蕉品种的改良提供了一种有效的手段。香蕉的遗传转化方法有电激法、基因枪法、农杆菌介导法等。农杆菌介导法的应用是香蕉品种改良的一个重大突破。香蕉遗传转化的外植体也发展到多种,有原生质体,胚性细胞悬浮系,分生组织,以及横切薄片等。近几年,随着分子生物学的发展,出现了转化效率更高,重复性更好的香蕉遗传转化技术。如农杆菌和基因枪结合法,离心辅助农杆菌介导法、真空渗透技术等。这些新技术新方法的出现,必将推动香蕉产业高速发展。     

壳聚糖对石榴果汁澄清效果的影响

利用壳聚糖的絮凝作用对壳聚糖在甜石榴汁澄清中的应用效果进行了研究,试验结果表明,壳聚糖用量为0.3g/L、澄清时间为60min、温度为35℃时,甜石榴汁透光率可达85%以上,且通过对壳聚糖处理前后可溶性固形物、总酸、VC等营养成分含量的比较,确定以上工艺参数为甜石榴汁澄清的最佳选择。     

新疆布尔津县土地利用格局特征研究

基于RS与GIS技术,利用景观生态学理论及景观格局指数对布尔津县土地利用现状进行定量分析。结果显示为：（1）布尔津县以草地、林地为主要景观类型,镶嵌着耕地、水域等景观类型。（2）四个分区（即高山区、丘陵区、平原区、荒漠区）的林地资源组成差异明显,耕地、居民、交通及工矿用地集中分布在丘陵区和平原区（3）从各项指数来看,高山区的多样性指数较低;平原区是受人文因素最多的区域,景观优势度低,各种土地利用类型比例差异不大,有利于生态可持续发展,但要合理的开发利用;荒漠区生态环境相对脆弱,要加强生态环境的保护与改善。通过该研究对布尔津县的土地利用现状作一个了解,为该县乃至整个地区的景观规划和可持续发展战略提供科学依据。     

酸枣幼根原生质体的制备

以生长6～9 d的酸枣种子幼根为试验材料,0.7 mol·L-1甘露醇作为渗透压稳定剂、pH值5.5～6.0、28℃恒温水浴条件下,以光学显微镜观察的水解总时间、初始产生原生质体的时间、原生质体产量及细胞碎片量为指标,研究通过酶水解来制备酸枣幼根原生质体的方法.结果表明,在含1％果胶酶+4％纤维素酶R-10+3％纤维素...     

影响核桃贮藏品质因素的分析

介绍了几种核桃贮藏方法,综述了影响核桃贮藏品质的几个主要因素,以及针对上述影响因素提出若干解决对策.     

The Simulation of Comfort of Nine DOF of Automobile

Automobile comfort dynamic simulation is necessary when we predict and evaluate the comfort of a automobile or optimize performance of automobile. The nine DOF vibration model of automobile is established , which acts root mean square value of body in multiple work conditions as comfort evaluation indices. Based on the comfort simulation, the Hongyan 1160 is predicted in the way of comfort. The programmes can be used in many different automobiles . The computational result indicates that the model can simulate vibration of automobile truly. It is significant to research automobile comfort evaluation to optimize performance of automobile and comfort simulation .The computational program can be applied to optimize or simulate which has some reference value.     

Efficiency of water use of early plantings of sunflower

Rain fed crop production in Mediterranean environments depends to a large extent on strategies that avoid the intense summer drought. Early plantings of sunflower have given consistently higher yields in such environments, but the basis for such yield increases has not been explored. We conducted two field experiments at Cordoba (Spain) to investigate the effects of an early and a late planting date on the components of water-limited crop productivity; namely, water use (T), water use efficiency (TE) and harvest index (HI) of sunflower. The results were generalized by simulating rain fed sunflower yields, under early (1 January) and late (15 March) plantings, for a 25-year period with the aid of a simulation model of the Ceres type (OILCROP-SUN) which has been validated in Cordoba. Experimental seed yields of early plantings in 1989 and 1996 were 2.0 and 3.0 t ha⁻¹, while late plantings yields were 1.3 and 2.4 t ha⁻¹, for the 2 years. Average simulated yields were 2.7 ± 1.1 and 1.9 ± 0.7 t ha⁻¹ for early and late plantings, respectively. For the 2 years, T of early plantings was higher than that of late plantings, but the response of TE and HI to planting date was not the same in the two experiments. In the simulation exercise, T and TE of early plantings were consistently higher than those of late plantings, while there were no differences in the HI for the two planting dates. We conclude that early plantings of sunflower increase rain fed yields by increasing both T and TE, while the impact of planting date on HI very much depends on the crop water stress pattern, which is quite variable from year to year even in the predictable Mediterranean environment.     

Establishment of High-frequency Regeneration System of Explants of Alfalfa

The cotyledon and hypocotyl of eight varieties of alfalfa( Medicago sativa L. )are used to study the effect of different cultivation procedures and genotypes on embryogenic callus and embryoid induction,plant development and transplant of alfalfa.The varieties of alfalfa with high embryoid induction rate and high differentiation rate are screened. A high frequency regeneration system for alfalfa tissue culture is established:which is benifical to the gene transformation of alfalfa.