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甘薯抗茎线虫病基因AFLP标记的开发 总被引:2,自引:0,他引:2
以甘薯(Ipomoea batatas)抗茎线虫病品种徐781和感茎线虫病品种徐薯18杂交F1分离群体的186株单株为材料,利用分离群体分组分析法(BSA法)和AFLP技术,在抗感池中共筛选了800对AFLP引物,结果表明,其中245对引物具有多态性.用这245对引物检测两亲本以及建池单株,发现引物组合E2M23和E33M20分别在抗病单株中扩增出l条在感病单株中未出现的特异条带,长度分别约为500和200 bp,认为这2个AFLP标记与甘薯抗茎线虫病基因连锁,分别命名为E2M23500和E33M20200.根据这2个AFLP标记对F1代186个单株的扩增结果,经Mapmaker 3.0软件分析,发现这2个分子标记与抗茎线虫病基因位于同一连锁群并紧密连锁,它们与抗茎线虫病基因间的遗传距离分别为6.9和11.1 cM.用这2个分子标记对10个中国甘薯主栽品种进行检测,所得结果与常规方法鉴定结果完全一致,表明2个分子标记可用于甘薯抗茎线虫病分子标记辅助育种. 相似文献
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刘庆昌 《农业生物技术学报》2008,16(5)
以甘薯抗茎线虫病品种徐781和感茎线虫病品种徐薯18的杂交F1分离群体的186株单株为材料,利用分离群体分组分析法(BSA法)和AFLP技术,在抗感池中共筛选了800对AFLP引物,结果表明其中245对引物具有多态性。用这245对引物检测两亲本以及建池单株,发现引物组合E2M23和E33M20分别在抗病单株中扩增出1条在感病单株中未出现的特异条带,长度分别约为500 bp和200 bp,认为这2个AFLP标记与甘薯抗茎线虫病基因连锁,分别命名为E2M23500和E33M20200。根据这2个AFLP标记对F1代186个单株的扩增结果,经Mapmaker 3.0软件分析,发现这2个分子标记与抗茎线虫病基因位于同一连锁群并紧密连锁,它们与抗茎线虫病基因间的遗传距离分别为6.94 cM和11.1 cM。用这2个分子标记对10个中国甘薯主栽品种进行检测,所得结果与常规方法鉴定结果完全一致,表明2个分子标记可用于甘薯抗茎线虫病分子标记辅助育种。 相似文献
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与黄瓜感花叶病毒病基因连锁的分子标记 总被引:1,自引:0,他引:1
本研究以黄瓜(Cucumis sativus L.)高感黄瓜花叶病毒(CMV)和高抗CMV亲本组合(HZL04-1×F-3)的F2分离群体为试材,采用极端个体分组法和AFLP技术筛选与黄瓜抗CMV基因连锁的分子标记.AFLP引物组合E22M88在抗病组和感病组间约200 bp处表现多态性.经F2单株分析,在感病亲本和感病单株中扩增出了约为200 bp的特异片段,而抗病亲本和抗病个体无此条带.该特异标记与CMV抗性基因相连锁,遗传距离为9.74 cM.测序结果显示,目标片段的大小为202 bp,并将该标记转换为了序列特征化扩增区域(SCAR)标记.提供了黄瓜材料CMV抗性鉴定的分子方法和手段,可用于分子标记辅助育种. 相似文献
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小麦抗秆锈突变系龙辐03D51的筛选及其抗病性的遗传分析与RAPD标记 总被引:2,自引:2,他引:0
以龙6239幼胚为外植体,利用辐射诱变和组织培养相结合的方法,选育出突变系龙辐03D51,经秆锈接种鉴定,发现其对优势小种21C3CPH免疫,而亲本龙6239对21C3CPH高度感染。遗传分析表明,抗病性由显性单基因控制。该突变系还具有其亲本的优质、高产特点,已成为优异的后备品系。在RAPD检测中,所用的60个随机引物中有3个引物在龙辐03D51和其亲本龙6239中具有多态性,引物E07、E11、E17在龙辐03D51中分别扩增出380bp、700bp和600bp的特异带,初步认为这些谱带可能与秆锈抗性有关。 相似文献
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摘要:本文以两个甜瓜杂交一代品种“东方蜜1号”、“东方蜜2号”及其亲本和若干同母异父的杂交组合为实验材料,利用SSR标记对甜瓜杂交种纯度快速鉴定的方法进行了研究。结果表明:SSR标记在甜瓜杂交种及亲本间具有较高的多态性,利用筛选出的SSR引物能够快速准确的区分出杂交种中混杂的母本自交系种子,对3批杂交种纯度的快速鉴定结果与大田鉴定结果一致。但是,利用筛选出的单一SSR引物不能完全区分开杂交种和与其同母异父的供试杂交组合,而用不同的引物组合能够将杂交种与大多数供试杂交组合区分开,但对于个别与杂交种遗传背景十分接近的杂交组合仍不能区分开。 相似文献
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山东省46个花生品种SSR指纹图谱构建与遗传多样性分析 总被引:2,自引:0,他引:2
为从分子水平上快速鉴别花生品种和选配优良杂交组合,以山东省审定的46个花生品种为材料,利用微卫星(SSR)标记进行DNA指纹图谱的构建和遗传多样性分析。从788对SSR引物中筛选出50对多态性高、稳定性好、谱带清晰的引物,共检测到175个等位位点,其中122个为多态性位点,多态性比率达70.52%;每对SSR引物扩增出的等位位点数为2~7个,多态性信息量变化范围为0.6753~0.8412,平均为0.823。此外,利用14对引物可将46份材料完全区分开。聚类分析表明,在相似系数0.77处,所有供试材料聚为一类,在相似系数0.80处,仍有76%的材料聚在一起。利用SSR标记构建的指纹图谱可为花生种质资源管理及育种实践提供依据。 相似文献
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Ujihara T Taniguchi F Tanaka J Hayashi N 《Journal of agricultural and food chemistry》2011,59(5):1557-1564
To develop cleaved amplified polymorphic sequence (CAPS) markers for cultivar identification of the tea leaf, 5 primer pairs designed on the basis of genes that encode proteins related to nitrogen assimilation and 26 primer pairs based on expressed sequence tag (EST) sequences of the root of tea plant were screened. From combinations of primer pair and restriction enzyme that showed polymorphism among tea plants, 16 markers were selected and applied to DNA fingerprinting of Japanese tea cultivars. Sixty-three cultivars, except for a bud sport (Kiraka) and its original cultivar (Yabukita) and a pair that was the progeny of the same crossing parent (Harumoegi and Sakimidori), were distinguished from one another. By combining the 16 markers with previously developed CAPS markers and observing the physical appearance, 67 cultivars were distinguishable. The cultivars involve approximately 95% of total tea cultivating area in Japan; therefore, about 95% of tea leaves produced in Japan can be authenticated by labeling their cultivars. 相似文献
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Sonia?Marghali Olivier?Panaud Fran?oise?Lamy Salma?Ghariani Aboubakry?Sarr Mohamed?Marrakchi Neila?Trifi-Farah
Amplified fragment length polymorphism (AFLP) markers were used to characterize the genetic diversity within and among natural populations and cultivars of Hedysarum coronarium. Twelve populations within Tunisia were evaluated with three AFLP primer combinations. A total of 207 reproducible bands was detected of which 178 (86%) were polymorphic. The great discriminative power of AFLP markers and their ability to represent genetic relationships among Hedysarum plants was demonstrated. Genetic diversity within and among populations was assessed through Principal Component Analysis (PCA) and cluster analysis by using the Neighbor-joining clustering algorithm. AFLP technology has provided evidence of a high degree of intra- and inter-population genetic diversity in H. coronarium. AFLP banding patterns provided molecular markers correlated with the plants’ geotropism. In addition, AFLP markers can differentiate wild accessions from cultivars. Moreover, geographical origins did not correspond to population clustering. 相似文献
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不同茶树品种(系)的绿茶滋味分析及评价模型构建 总被引:1,自引:3,他引:1
为客观准确地评价不同茶树品种(系)绿茶的滋味品质,该研究系统分析了25个茶树品种(系)绿茶的主要滋味成分含量及其Dot值,利用主成分分析法对不同茶树品种(系)绿茶滋味品质进行综合评价并建立滋味品质评价模型。结果表明:儿茶素、咖啡碱是不同茶树品种(系)绿茶苦涩味强度差异的主要原因,其中表没食子儿茶素没食子酸酯(Epigallocatechingallate,EGCG)是所测样品中涩味的主要贡献物质,EGCG和咖啡碱为所测样品中苦味的主要贡献物质。氨基酸是不同茶树品种(系)绿茶鲜味、甜味差异主要因素,谷氨酸是所测样品中鲜味的主要贡献物质。主成分分析表明:前5个主成分的累计方差贡献率为79.018%,并以前5个主成分的线性回归方程和贡献率构建了滋味品质评价模型,模型评价结果与感官审评结果较为相似,存在极显著相关性(P0.01),相关系数为0.791;利用模型中各主成分的得分,能够较好的区分所测样品的苦、涩、鲜、爽等滋味特征。因此,该研究所建模型能够较好的评价所测样品的滋味品质及其主要滋味特征的差异,为各茶树品种(系)的推广应用提供理论依据,也为不同茶树品种(系)绿茶滋味品质的科学评价提供新的思路和方法。 相似文献
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小菊自交种子辐射生物学效应的研究 总被引:7,自引:0,他引:7
利用60 Coγ射线对 7个小菊品种的自交种子以 1 5Gy的剂量进行辐射处理 ,对后代的株高、花径、花色和花期等变异情况进行观察统计。结果发现 ,M1代植株平均株高明显矮化 ,获得了一批株高小于 30cm的植株 ;花径与亲本相比发生了广泛分离 ,花期因品种不同而可能推迟或提前。花色容易发生变异 ,仅有黄花和红花品种在M1代中分别有 44 9%和 48 3%的植株保留了亲本花色 ,其它花色在M1代中都不具有优势 ;除了红色花品种变异谱较窄外 ,其它花色变异谱都较宽。 相似文献
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利用AFLP标记和形态性状检测皖北小麦主栽品种的遗传多样性 总被引:2,自引:1,他引:1
利用AFLP标记和2年田间试验对皖北地区小麦主栽品种的遗传多样性进行了研究。以AFLP标记在20个品种之间扩增的172条多态性位点数据,采用Nei和Li的方法,计算品种间的遗传距离;同时,以2年田间试验得到的品种株高、穗长、小穗数、小穗密度、千粒重、护颖长短、粒长和粒宽等表现型性状平均数经正态标准化后,采用欧氏距离计算品种间遗传距离。基于分子标记的聚类分析结果与系谱分析基本一致,把系谱来源不清的品种划分到相应的类群。若以整个遗传距离的总平均数作尺度对聚类图的结果进行分类,大致可分为6类。Mantel检测表明,AFLP标记数据计算的遗传距离矩阵和表现型计算的遗传距离矩阵存在极显著的相关性(r=0.8260,t=11.325),证明AFLP标记是检测品种间遗传差异的有效方法,可为小麦育种亲本选配提供理论依据。研究还证实,一个骨干亲本与由其衍生出来的品种(系)之间的遗传差异一般较小。 相似文献
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To evaluate the genetic diversity and to clarify the genetic relationships of Japanese peach cultivars, we analyzed the amplified
fragment length polymorphism (AFLP) and traced the pedigree of 17 Japanese commercial peach cultivars and six traditional
accessions. Sixteen AFLP primer combinations produced a total of 837 fragments and 146 polymorphic bands with a polymorphism
percentage of 17.5%. All of the peach accessions could be identified from differences in at least 10 polymorphic bands. A
cluster analysis showed that all the Japanese commercial peach cultivars, except ‘Kiyomi’ and ‘Jichigetsuto’, formed a major
group consisting of three sub-groups. Of the six traditional accessions, four were genetically distant from the Japanese commercial
peach cultivars while two accessions from China were classified into the Japanese commercial peach cultivars group. Both the
AFLP analysis and pedigree tracing suggested that Japanese commercial peach cultivars are mainly derived from ‘Shanhai Suimitsuto’,
one of the traditional accessions from China. Although the genetic relationships revealed by AFLP were generally in agreement
with those shown by the pedigree information, some contradictions were found. Combining the AFLP results and pedigree information
can provide a better understanding of the genetic relationships of Japanese peach cultivars. 相似文献
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N. Grati-Kamoun F. Lamy Mahmoud A. Rebaï A. Gargouri O. Panaud A. Saar 《Genetic Resources and Crop Evolution》2006,53(2):265-275
About 29 olive (Olea europaea L.) cultivars including oil and table olive cultivars originating from Tunisia and other Mediterranean countries, were genotyped
using amplified fragment length polymorphism (AFLP) DNA markers. This technique is a rapid and efficient method for producing
DNA fingerprints. Using nine AFLP primer combinations, we produced a total of 410 AFLP markers, among which 172 revealed polymorphism.
The results demonstrated a high degree of polymorphism in the olive germplasm we examined with an average of 39%. These AFLP
markers were analyzed to estimate genetic distances between pairs of cultivars using Jaccard’s similarity coefficient. Furthermore,
cluster and principal component analyses were performed in order to identify the genetic variation patterns. Two main groups
were obtained: one comprising primarily small-fruited cultivars grown mainly for oil production and the other comprising large
fruited cultivars (regardless of their end-use). Our results show no evidence of clustering of olive cultivars according to
their geographic origin. 相似文献
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本研究以毛蟹(C.sinensis cv.Maoxie)、本山(C.sinensis cv.Benshan)、梅占(C.sinensis cv.Meizhan)3个乌龙茶品种为研究对象,分析不施肥条件下茶树根部土壤养分随种植年限的变化,以及茶叶品质的变化。结果表明,不同茶树的根部土壤p H值均随茶树种植年限的增长而呈现下降趋势。不同茶树根部土壤的有机质及有效磷含量达到Ⅰ级肥力;0年取样的茶树土壤碱解氮含量为Ⅰ级肥力,不施肥种植1~2年后均为Ⅱ级肥力;土壤速效钾含量在茶树0年取样时为Ⅰ级肥力,不施肥种植1年后毛蟹根部土壤为Ⅲ级肥力,本山和梅占则为Ⅱ级肥力,2年后均为Ⅲ级肥力。茶树叶片品质分析结果表明,随着不施肥种植时间的延长,茶叶中茶多酚、茶氨酸、咖啡碱的含量呈现下降趋势。同一种茶树在不同种植年限间,茶多酚含量的变化较为微弱,而茶氨酸、咖啡碱含量则呈显著差异。不同种植年限下茶树叶片中表没食子儿茶素没食子酸酯、表儿茶素没食子酸酯与表没食子儿茶素含量及其品质指数均随着不施肥种植时间的延长而呈下降趋势,且同一种茶树不同种植年限间的变化率均呈显著差异。 相似文献
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本文运用AFLP分子标记技术,对江西省4个斑点叉尾鮰(Ictalurus punctatus)养殖群体(GZ、XJ、PYA和PYB)进行遗传多样性分析。结果表明,在64对引物组合中,E3/M2、E4/M7、E3/M8和E5/M5引物从4个群体72个体中共扩增出179个位点,其中多态位点为109,占60.89%。其中,PYA、GZ、XJ和PYB群体的多态位点比例(P)分别为56.54%、56.47%、56.32%和56.14%。Shannon多样性指数(I)分别为0.2890、0.3003、0.2896和0.2852,平均杂合度(H)分别为0.1935、0.2027、0.1938和0.1898。4个群体间的遗传分化系数(Gst)为0.1314,说明群体间发生了一定程度的遗传分化,但分子方差分析(AMOVA)显示,群体的遗传变异90.98%来源于群体内的个体间,9.02%来源于群体间。聚类分析也表明,4个群体所产生的遗传分化主要也来源于群体内。本研究为斑点叉尾鮰种质资源的调查和保护提供参考,为斑点叉尾鮰优良品种的选育提供科学依据。 相似文献