Reinke's Crystals in an Interstitial Cell Tumour of a Rabbit (Oryctolagus cuniculus)

Contents One case of interstitial cell tumour in the testicle of a New Zealand White rabbit was reported. The rabbit was 9-years old and had monolateral testicular lesion. There was enlargement of left testis and the lesion were seen on the cut surface. Light microscopy revealed intracytoplasmic crystals of Reinke that resembled to the features observed for this tumour in human. Immunohistochemically, this tumour was positive for vimentin.     

Haemorheological Variables in a Rat Model of Hypertriglyceridaemic Obesity and Diabetes

Obesity is a heterogeneous condition of variable aetiology, generally associated with pathologies such as arterial hypertension, hyperlipidaemia, diabetes and cardiac disease. These conditions, either themselves or because of the various treatments used, may further modify blood rheology in an arbitrary manner. Therefore, analyses of changes in the blood rheology induced by obesity in humans have had differing and controversial results. In our laboratory, a model of hypertriglyceridaemic obesity is provided by an inbred rat strain; the genotype from the IIMb/Fm strain, presenting a syndrome of moderate obesity with apparent peripubertal onset, associated with hypertriglyceridaemia and glucose intolerance that turns into diabetes. The genotype, originated from the same IIM/Fm stock, represents the control.The present study describes a comparative analysis of the variables determining the rheological behaviour of the blood in obese and control strains. Our results, agreeing with some other studies performed in humans, confirmed the haemorheological changes associated with obesity, and the fact that these changes became more evident in the presence of pathologies such as diabetes. It appears that triglyceridaemia, cholesterolaemia and hyperglycaemia may influence the rheological behaviour of the cell membrane and this damage may provoke a decrease in erythrocyte deformability and, consequently, hyperviscosity of the blood.     

子宫内膜异位症大鼠模型复制

取Wistar大鼠30只,用手术移植的 方法把自体子宫内膜组织2块分别移植到壁 腹膜与子宫系膜处,建立子宫内膜异位症大 鼠模型。术后4周,再次开腹,观察异位内膜 的存活情况、与周围组织的粘连程度及病理 学变化。结果发现腹膜和子宫系膜处移植内 膜的成活率及病灶的体积差异无显著性(P >0.05),腹膜处病灶与周围组织的粘连程度 显著轻于子宫系膜处病灶(P<0.01),移植 物外观呈囊状,表面有血管,光镜下观察见子 宫内膜细胞、间质细胞、腺体和血管,与正常 在位内膜相比腺体减少。该模型复制方法简 便,成功率高,是研究子宫内膜异位症较理想 的动物模型。     

大鼠骨肉瘤模型的建立

为建立大鼠骨肉瘤动物模型的方法, 用30只6周龄SD大鼠随机分为3组,将大 鼠骨肉瘤细胞系UMR 106分别以105,106, 107个数量注入大鼠股外侧皮下。接种后,每 周观察肿瘤生长情况,测量肿瘤直径,绘制肿 瘤生长曲线,连续5周。第6周取肿瘤病理 材料,常规切片,HE染色,光镜下观察。结果 105个细胞及106个细胞组肿瘤生长情况不 佳;107个细胞组肿瘤发生率达到100%。说 明大鼠骨肉瘤细胞系UMR 106具有较强的 致瘤性,应用该细胞系建立的动物模型为骨 肉瘤综合治疗奠定了动物实验基础。     

Malignant Mixed Sex Cord-stromal Tumour in a Stallion

A 30-year-old Standardbred stallion was examined for unilateral scrotal swelling. Physical and ultrasound examinations revealed a painless enlarged left testis with a non-homogeneous echogenicity, when compared with the controlateral testis. The stallion underwent left unilateral orchiectomy. Grossly, the excised testis was irregularly enlarged (12 x 9 x 9 cm; weight: 530 g) and firm. The sections showed that testicular parenchyma was replaced by a lobulated, greyish-white mass, which involved the epididymal head. At microscopy, a dual Leydig and Sertoli cell tumour component could be seen. Neoplastic Sertoli cells were prevalent and presented pleomorphic cells, mitotic figures and occasional vascular invasion. Tumour patterns showed tubular and solid areas, cord-like or diffuse in appearance, among which newly formed Leydig cell nests and low-density fibrillar bundles were interposed. Immunohistochemically, a weak to moderate immunostaining for vimentin, AE(1)/AE(3) cytokeratin, alpha-1-antitrypsin and CD99 antigens was found in the growing Sertoli cells, whose nuclear MIB-1 labelling index scored 13 +/- 2%. The Leydig tumour cells, on the other hand, displayed a moderate to strong positivity for alpha-inhibin, vimentin, AE(1)/AE(3) cytokeratin, neurone-specific enolase and CD99. On the basis of these findings, a diagnosis of malignant mixed sex cord-stromal tumour was made.     

Bilateral Testicular Leydig Cells Tumour in a Donkey

A rare case of a bilateral Leydig cells tumour located in the testis of a 15-year-old donkey slaughtered normally is presented. Histologically, the testicular tumour showed neoplastic Leydig cells arranged in solid growth as a pseudoadenomatous packet type. To our knowledge, this is the first bilateral Leydig cells tumour, a neoplasm never reported in the donkey.     

Dose-effect Study of Lipoic Acid Supplementation on Growth Performance in a Weaned Rat Model

The present study investigated the doseeffect relationship of graded levels of lipoic acid supplementation on growth performance and small intestinal development in a weaned rat model.Seventy-two weaned Sprague-Dawley rats,were fed semipurified diets(n=12),either unsupplemented(group Ⅰ)or supplemented with 12.5,25,125,or 250 mg/kg body weight(BW)fipoic acid(groups Ⅲ,Ⅳ,Ⅴ,and Ⅵ),with 200 mg/kg BW aureomycin as the antibiotic control(group Ⅱ).The experiment lasted 21 days.Growth performance was not significantly different (P＞0.05)between rats under the antibiotc control (group Ⅰ)and rats fed low levels(12.5 and 25 mg/kg BW)of lipoic acid(groups Ⅳ and Ⅳ).In contrast,high level(125 and 250 mg/kg BW)lipoic acid supplementation(groups Ⅴ and Ⅵ)(P＜0.05)reduced weight gain,feed consumption,and feed efficiency.In addition,high levels(125 and 250 mg/kg BW)of lipoic acid significantly(P＜0.05)reduced the villus height/crypt depth ratio,as well as the numbers of lactobacillus.total aerobes.and total anerobes in the gastrointestinal tract compared with the other treatments,which meant that high levels of lipoic acid impaired intestinal morphology and disordered the balance of intestinal microbiology.Furthermore,the results showed that high leveIs of lipoic acid supplementation(P＜0.05)elevated interferonγ and intefleukin-2,but dramatically(P＜0.05)depressed interleukin-4 and interleukin-10 compared with the low levels of lipoic acid supplementation and the control group,which indicated that high levels of lipoic acid would induce bias of Th1/Th2 lymphocytes.Taken together,the results indicate that lipoic acid supplementation can't improve growth performance and intestinal development of normal animals,especially,high levels(≥125 mg/kg BW)of lipoic acid supplementation restrained growth performance and intestinal development,in association with unbalance of certain cytokines.     

幼龄大鼠环磷酰胺免疫抑制模型中血液学和肠道黏膜免疫指标的观察

为了解幼龄大鼠环磷酰胺免疫抑制模型血液学和肠道黏膜免疫相关指标的变化特点,本研究观察与比较了4周龄雌性SD大鼠在体重、临床表现、血液学、血清生化指标以及肠道黏膜免疫等方面的变化。与对照组相比,幼龄大鼠体重增长缓慢(P<0.05);外周血白细胞总数、嗜中性粒细胞数、淋巴细胞数、单核细胞数与网织红细胞数显著降低(P<0.05);血清球蛋白含量显著减少(P<0.05);小肠派氏结数、杯状细胞数、肠道内淋巴细胞数和分泌型Ig A量均显著减少(P<0.05);其余血清生化指标以及肠道绒毛发育未见显著影响。研究表明,幼龄大鼠固有免疫与获得性免疫水平在模型中均受到显著抑制,后期固有免疫较获得性免疫恢复程度好。免疫指标中外周血细胞数及小肠派氏结数推荐在模型应用中优先选择。     

Interstitial lung fibrosis and emphysema in a standardbred horse: A case report

A horse with severe work-intolerance was examined. Neither clinical nor radiographic examination suggested the presence of emphysema and lung fibrosis.     

Interstitial cell tumor and Sertoli cell tumor in the testis of a cat

Testicular tumors are rarely reported in cats. We describe a case of interstitial cell tumor and Sertoli cell tumor in a cat that developed aggressive behavior and inappropriate urination 7 years after it was obtained from a shelter as an allegedly castrated 2 year old. At physical examination, the urine odor and the presence of penile papillae implied testosterone production. Testes were not palpable, but the left testis was found in the scrotum by surgical exploration and was mostly replaced by the 2 tumors. The interstitial cell tumor, but not the Sertoli cell tumor, was immunohistochemically positive for Melan-A, consistent with steroid production. Behavior improved after excision of the testis and penile papillae began to regress, but the cat was euthanatized 3 1/2 months after castration at the owner's request. Neither tumor had metastasized. The right testis was never found and was presumed to have been removed during the reported castration procedure.     

Experiences and Skills for Establishment of Rat Model of Myocardial Ischemia and Reperfusion

The establishment of rat model of myocardial ischemia and reperfusion is a very sophisticated experimental process. During animal experiments, beginners always meet with troubles inevitably. This paper have conducted a great deal of this type of experiments for years, and intend to share some experiences and lessons with colleagues.     

Tumour suppressor gene p53 mutation in a case of haemangiosarcoma of a dog

Haemangiosarcomas of dogs were analysed by molecular genetic techniques. Regions of the tumour suppressor gene p53, including the well-known tumour hot spots (codons 175, 245, 248, 249, 273 and 282) were screened. A 24 bp deletion was detected in exon 5 of the gene.     

Angiogenesis and Vascular Regression in the Corpus Luteum Periodicum

Introduction and Aim: The corpus luteum is one of the most intensely vascularized tissues. Luteal angiogenesis is strictly controlled and blood vessels regress completely within a short period of time. The aim of this study was to investigate vascular dynamics in relation to cellular and molecular mechanisms of luteal angiogenesis and anti‐angiogenesis. Material and Methods: Endothelial cells of blood vessels in paraffin sections of bovine corpora lutea from different stages were examined by labelling with the lectin Bandeiraea simplicifolia agglutinin I. Angiogenesis was studied by morphometry of the capillaries, and immunolocalization of the angiogenic factor VEGF and VEGF‐receptor 2. Presence of apoptotic luteal and endothelial cells was investigated using the TUNEL test and transmission electron microscopy. Results: During development of the corpus luteum (day 3–8 of the oestrous cycle) a dense capillary network (8–12% area ratio) is established and maintained until day 17. Early regression (day 18–24) is characterized by a remarkable decrease of capillaries (1% area ratio). In the regressing corpus luteum the number of apoptotic luteal cells is closely correlated (r = 0.9) to the number of apoptotic endothelial cells. VEGF is immunolocalized in luteal cells (day 3–17), smooth muscle cells and endothelial cells of arterioles of the regressing corpus luteum. During late luteal regression, a moderate increase of capillaries (2.5% area ratio) is obvious. Conclusions: The dynamic changes of the capillarity during development and regression of the cyclic corpus luteum correlate with VEGF and VEGF‐R2 activities. In contrary to expectations the late stage of luteal regression is accompanied by angiogenesis. One reason for this phenomenon may be an increase in metabolic activity resulting in re‐organization of blood vessels already regressed.     

Cofilin在癌细胞迁移中的作用与调控

Cofilin(丝切蛋白)是肌动蛋白解聚因子家族的重要成员,其磷酸化状态直接决定对丝状肌动蛋白的切割活性,从而在膜突的形成及细胞迁移中起到重要的作用,并且与癌症的转移密切相关.在多种癌症细胞中,Cofilin受到复杂的调控,尽管尚不能直接应用于癌症的治疗,但一些相关机制已经形成了体系,这些理论将在本文中详细阐述.     

Splenic Hemangiosarcoma in a Young Sprague-Dawley Rat

The present report describes a rare case of spontaneous hemangiosarcoma in a nine-week-old male Sprague-Dawley rat. At necropsy, multiple white nodules of various sizes were observed on and within the enlarged spleen and liver and were histopathologically determined to be composed of spindle- to oval-shaped cells that showed invasive growth without encapsulation and were arranged solidly but partially in whorls or faint alveolar patterns with vascular-like spaces containing small clefts or erythrocytes in the tumor mass. Immunohistochemical analysis revealed that most of the tumor cells were strongly positive for vimentin, von Willebrand factor (vWF) and CD34 but negative for podoplanin. In addition, electron microscopic examination revealed the presence of Weibel-Palade bodies in the cytoplasm of the tumor cells. Based on these findings, this case was diagnosed as a hemangiosarcoma. The splenic masses were larger than the hepatic ones, with tumor cells mainly observed at periportal regions with tumor embolism in the liver, suggesting that primary hemangiosarcoma initially developed in the spleen before metastasizing.     

Persistent Mullerian Duct Syndrome in a Miniature Schnauzer Dog with Signs of Feminization and a Sertoli Cell Tumour

A 5‐year‐old male Miniature Schnauzer was presented with unilateral cryptorchidism and signs of feminization. Abdominal ultrasonography revealed an enlarged right testis and a large, fluid‐filled cavity that appeared to arise from the prostate. Computed tomography revealed the cavity to be consistent with an enlarged uterine body, arising from the prostate, and showed two structures resembling uterine horns that terminated close to the adjacent testes. The dog had a normal male karyotype, 78 XY. Gonadohysterectomy was performed and both the surgical and the histological findings confirmed the presence of a uterus in this male animal, resulting in a diagnosis of persistent Mullerian duct syndrome (PMDS). The enlarged intra‐abdominal testis contained a Sertoli cell tumour. Computed tomography proved to be an excellent diagnostic tool for PMDS.     

向下并流压力式喷雾干燥过程数学模型及干燥塔设计软件

通过对压力式喷雾干燥雾滴形成机理及受力分析,运用流体力学和传热传质学对干燥过程进行数学描述。由此可为压力式喷雾干燥雾滴在塔内的干燥时间,干燥空气用量,干燥塔高和直径的确定及喷嘴设计提供依据。在此基础上,编写了向下并流式喷雾干燥的VisualBasic程序。该程序在输入不同的喷雾压力,干燥气体性质等基本参数后,就可得出相应的不同干燥时间,干燥空气用量,喷嘴及干燥塔的结构尺寸等参数。软件计算结果与生产乳品用干燥机实际参数比较,符合情况良好。从而实现了数学模拟优化实验,为干燥机的设计与选型提供了方便。在能源与所处理物料的最优化组合,节约能源上做出了尝试。     

Interstitial cell tumor in a black-and-white ruffed lemur (Varecia variegatus variegatus).

A 14.5-yr-old, male black-and-white ruffed lemur (Varecia variegatus variegatus) presented for acute enlargement of the left testicle and hemiscrotum. Physical examination also revealed poor pelage quality with short guard hairs, sparse undercoat, and areas of alopecia. Increased aggression was also reported. A unilateral, open orchiectomy was performed, with the left testicle, epidydymis, associated vaginal tunic, and attached spermatic cord removed. Microscopic evaluation was consistent with an interstitial cell tumor, with many morphologic features similar to this neoplasm in people. No overt histopathologic criteria of malignancy were present. Following orchiectomy, gradual improvement in pelage quality was noted and was considered almost normal by 5 mo postoperative. In contrast with the aggressive preoperative behavior, the lemur was extremely submissive for 3 mo following the surgery. Gradual return to normal behavior and social status occurred over the next 2 mo. Multiple follow-up examinations and radiographs revealed no evidence of metastasis, and biopsy of the remaining testicle 4 mo later revealed no evidence of neoplasia. Serial measurements of testosterone and estradiol revealed levels within the range of those for other ruffed lemurs, as were repeated measurements taken of the remaining testicle. At 19 mo postoperative, the lemur had a coat quality considered nearly normal and maintained its historical social position in the lemur group without abnormal aggressive behavior.