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1.
镉对鲤血清促性腺激素和生长激素的影响   总被引:3,自引:0,他引:3  
马广智 《水产学报》1995,19(2):120-126
采用CdCl2浸没法处理鲤,研究镉对鲤血清促性腺激素(GtH)和生长激素(GH)水平的影响,结果表明,较镐浓度镉(CdCl29mg/L)处理引起血清GtH水平降低,GH水平升高,提高水中钙浓度,能使由镉引起的GtH水平的降低恢得取正常水平,并对镉引起的GH水平升高起延迟作用,镉处理使鲤对LHRH-A刺激GtH分泌的反应性下降,提高水中钙浓度也不能恢复正常,实验结果提示,镉引起的GtH和GH的变化可  相似文献   

2.
The main indication of cimetidine is being H2-receptor antagonist, but studies suggest that cimetidine may also act as a non-specific stimulant of cell-mediated immunity and immunomodulator. In order to determine the immunomodulatory effect of dietary intake of cimetidine in the common carp (100 ± 10 g), subjects were fed diets containing 0 (control), 50, 100 and 200 mg cimetidine kg?1 of dry diet for a period of 6 weeks. TLC and NBT assays were significantly (P < 0.05) stimulated in cimetidine-supplemented groups displaying the highest value in 200 mg kg?1 group. A decrease (P < 0.05) in cortisol and ACH50 value was recorded in fish treated with cimetidine. Serum protein, albumin and serum globulin levels were not significantly changed. The findings of the present investigation suggest that the incorporation of cimetidine in the diet of common carp enhances the non-specific immunity.  相似文献   

3.
Common carp juveniles were fed for 9 weeks one of the eight semipurified diets containing graded levels of magnesium, 0.08, 0.6, 1.1, 3, 2 g Mg kg–1 and 25 or 44% protein.Fish growth and feed utilization were significantly affected by both Mg and protein levels in the diets. Significant interaction between these two studied variables existed in relation to the fish performance as well as to mean deposition rate of several minerals in common carp body. The fish fed diets containing 0.08 g Mg kg–1 had reduced growth and developed deficiency signs such as muscle flaccidity and skin hemorrhages.Results indicated that a minimum Mg level of 0.6 g Mg kg–1 was required to elevate plasma and bone magnesium content and to reduce the whole body Ca concentration (hypercalcinosis symptom). Further increase of dietary Mg up to 3.2 g Mg kg–1 improved growth rate of fish insignificantly, but the deposition rate of dietary Mg fell to as low as 7.4 and 10.7 percent in low- and high-protein diet fed fish, respectively. In Mg-deficient fish, considerable amount of magnesium was absorbed via extra-oral routes, however, this way of the covering magnesium need becomes insufficient in fast growing fish.  相似文献   

4.
One-hundred-and-eighty-day bioassays were made in outdoor cement tanks (volume 300 L) with common carp, Cyprinus carpio (L.), exposed to eight different treatments, each with three replicates. Four treatments contained 2.5 mg L?1 cadmium (Cd): Cd alone; Cd with 6.7 g L?1 of compost manure made from the freshwater macrophyte Pistia stratiotes (L.); Cd with 1.0 g L?1 of the same manure; and Cd with a cumulative treatment of the manure (1.0 g L?1 month?1). Four treatments contained no Cd: the control (without manure); and treatments with only manure (6.7 g L?1,1.0 g L?1 and 1.0 g L?1 month?1). The concentration of dissolved (filterable) Cd in water ranged from 0.74 to 0.89 mg L?1 after 24 h (one day) of treatment. The addition of compost manure significantly reduced the Cd concentration of the water and induced a quicker removal of Cd from the water. The quickest removal of Cd from water (60 days) was found when a high dose of compost manure (6.7 g L?1) was added. The cumulative (monthly) treatment and the one-time treatment of the manure at a low dose (1.0 g L?1) required 75 and 90 days, respectively, for removal of Cd, while the metal persisted for up to 120 days in water when no manure was added. All doses of compost manure significantly reduced the bioaccumulation of Cd in plankton, but maximum reduction was found under cumulative treatment at a low dose of the manure. A single treatment at a high dose or cumulative treatment at a low dose of the manure helped in reducing the total body burden of Cd. A dose of 2.5 mg L?1 Cd did not show any adverse effect on the growth of fish, but compost manure, irrespective of its dose and combination with Cd, markedly increased the growth of fish and primary productivity of water. On the other hand, a high dose of manure resulted in much higher levels of NH3-N, N02-N and P04-P in the water in comparison with other treatments  相似文献   

5.
Six male carp, caught in the water system surrounding the Anna Paulowna (AP) Polder in The Netherlands, were characterized using allozyme and microsatellite markers. At the sMDH‐A1,2* loci an allele was found, which has previously only been found in wild River Rhine and wild Vietnamese common carp. Microsatellite allele frequencies showed that these AP carp were significantly different from a group of carp originating from several different domesticated strains. Based on both allozyme and microsatellite data, the AP carp probably originated from a wild or feral self‐sustaining population.  相似文献   

6.
鲤与生长性状相关的EST-SSRs标记筛选   总被引:4,自引:0,他引:4  
以大头鲤(Cyprinus pellegrini Tchang)(母本)与荷包红鲤(Cyprinus carpio wuyuanensis)抗寒品系(父本)杂交产生的F1作为亲本,以其自交F2作为分离群体,结合"拟测交"策略,用EST-SSRs标记对其中92个个体进行基因型检测.利用Windows Map Manager 2.0的标记回归法对符合拟测交分离的70个EST-SSRs标记进行单标记回归分析,检测出8个与鲤体长、体高、体厚性状相关的标记.对同一标记不同基因型个体间生长性状进行显著性比较,通过t检验,找到与生长性状相关的基因型.将上述8个EST序列与GenBank数据库进行BLAST比对,有3条EST序列与蛋白库中已知功能的序列高度同源.其中HLJE225与编码斑马鱼(J9enio rerio)冷诱导基因RNA结合蛋白的基因同源性水平高达97%,HLJE222与编码斑马鱼DAZ相关蛋白1基因同源性水平也高达97%,HLJE599与编码斑点叉尾鲴(Ictalurus punctatus)核糖体蛋白L6基因的同源水平为87%.本研究鉴定的与鲤生长性状相关的功能基因及有利用价值的基因型,为鲤重要生长性状的QTL(数量性状基闪座)定位和分子标记辅助育种提供了一定的依据.[中国水产科学,2009,16(1):15-22]  相似文献   

7.
This study investigated the effect of dietary bamboo charcoal (BC) supplementation on the growth performance and health status of juvenile common carp (Cyprinus carpio L.). A 63‐day feeding trial was carried out wherein the carp were reared on diets containing increasing levels of BC (0%, 0.5%, 1%, 2% and 4%). Treatment with BC did not produce any obvious effect (p > .05) on the growth performance of the juvenile common carp and on the overall muscle fatty acid composition. However, significant improvements (< .05) were reported on serum indicators such as alanine aminotransferase (ALT), aspartate aminotransferase (AST), total protein (TP), triglycerides (TG), total cholesterol (TC), high density lipoprotein (HDL) and glucose (GLU) with the 4% inclusion level producing the most beneficial effects without adversely affecting some of the indices that were not improved at this level. Improvements were further observed in the intestinal villus length and goblet cells numbers at 4% supplementation. Although some evidence of potential toxicity exists, the overall findings of this study suggest that supplementation of common carp diets with 4% BC has a potential to enhance serum biochemical indices, intestinal functions and fatty acid content in common carp. Furthermore, while no positive effects were observed on the growth performance and total muscle fatty acid composition, no adverse effects were reported on these parameters. Therefore, BC has the potential to be used as a feed additive in the diets of juvenile common carp to improve health status and intestinal function.  相似文献   

8.
The effect of reproduction was investigated on females of Hungarian strain W, French strain F, and their cross‐breed 1X whose ovulation was stimulated with carp pituitary (0.3 mg kg?1and, after 12 h, 2.7 mg kg?1) or Ovopel (one‐fifth of a pellet per kg and, after 12 h, one pellet per kg). It was found that in the case of Ovopel treatment, the percentage of spawning females of strain F and the cross‐breed 1X was higher than in the hypophysed fish compared. The applied ovulation stimulators did not significantly affect the weight of obtained eggs, whereas the significant (P ≤ 0.01) effect was recorded with respect to the quality of eggs after 12‐, 24‐, 36‐ and 48‐h of incubation. After Ovopel stimulation, the quality of eggs was better. The origin of the females had no statistically significant effect on the weight of eggs although the yield of eggs from fish of strain W was much smaller than that from females of strain F and the 1X cross‐breed. The interaction between the ovulation stimulator and the provenance of the females was significant (P ≤ 0.05) for the percentage of live embryos after 48‐h of incubation of eggs. Eggs of the best quality (and highest weight) were obtained from fish of strain F and cross‐breed 1X treated with Ovopel. In females of strain F that spawned within 6 and 10 h after the second Ovopel injection, the effect of the ovulation time on the weight of eggs was non‐significant. It was significant with respect to the percentage of egg fertilization and of live embryos after 36‐h of incubation (P ≤ 0.01 and P ≤ 0.05 respectively). The better quality of eggs (and their higher weight) was recorded when this time was shorter.  相似文献   

9.
The aim of the study was to assess the effects of a cyanobacterial extract containing microcystins (MCs) on selected hematological and biochemical parameters in common carp (Cyprinus carpio L.), as well as to determine the accumulation of toxins in fish tissues. The fish were immersed for 5 days in water containing toxins at a final concentration of 12 μg/L of microcystin LR equivalent. Microcystin LR residues were detected in fish liver, reaching 207, 238 and 260 ng/g f.w. of the tissues taken 24 h, 72 h and 5 days after the end of intoxication, respectively. The most substantial changes were found in fish plasma, including increases in creatine kinase, lactate dehydrogenase, ammonia, glucose, aspartate aminotransferase and alanine aminotransferase levels. A decline of about 50% in lysozyme activity was observed by the end of the experimental period. Moreover, a marked increase in ceruloplasmin activity was detected 24 h after the end of intoxication with a subsequent decrease in its activity after 72 h and 5 days. This study concludes that not only consumption of food containing toxins but also MCs dissolved in water may pose a threat to fish health. Additionally, detected changes in lysozyme and ceruloplasmin activity may have distinct effects in fish resistance against pathogens or oxidative stress, which should be taken into account in the future studies.  相似文献   

10.
This experiment was conducted to evaluate the effects of dietary pyridoxine on disease resistance, immune responses and intestinal microflora of fish. A total of 1050 Jian carp (11.71 ± 0.05 g) were randomly distributed into seven groups, feeding diets containing graded levels of pyridoxine (0.2, 1.7, 3.2, 5.0, 6.3, 8.6 and 12.4 mg kg?1 diet). After 80 days of feeding, a challenge trial was conducted by injection of Aeromonas hydrophila for 17 days. Results indicated that with increasing dietary pyridoxine concentration up to 5.0 mg kg?1 diet, survival rate after challenge with A.hydrophila and phagocytic activity of leukocyte were improved (P < 0.05), and plateaued thereafter (P > 0.05). Red blood cell and white blood cell counts were lowest when fed the diet containing 1.7 mg pyridoxine kg?1 diet. Haemagglutination titre, lysozyme activity, acid phosphatase activity, total iron‐binding capacity, antibody titre and immunoglobulin M content followed the similar pattern to that observed with survival rate. Aeromonas hydrophila, Escherichia coli and Lactobacillus counts in intestine were not affected by dietary pyridoxine concentration (P > 0.05). These results suggested that pyridoxine could enhance immune response of fish.  相似文献   

11.
In this study the effects of commercial juniper berry oil (JBO) as a feed additive in diets for common carp (Cyprinus carpio) was investigated in terms of growth performance, feed utilization and blood parameters. The experimental diets were prepared to contain JBO at rate of 0 (JBO‐0), 5 (JBO‐5) and 10 (JBO‐10) ml/kg. Common carps (3.07 ± 0.15 g) were fed with test diets for a period of 60 days in 40‐L aquariums of triplicate groups. The feeding experiment revealed that JBO groups showed significantly better growth performance and feed utilization compared with the control group (JBO‐0) (p < 0.05). No significant effect was observed on hemoglobin amount, haematocrit ratio and consequential erythrocyte indices (p > 0.05). In the JBO groups, serum cholesterol, triglyceride, glutamic–pyruvic transaminase and alkaline phosphatase levels decreased significantly (p < 0.05). In contrast, the total protein level showed a significant increase, while glucose, albumin and lactate dehydrogenase levels did not present any significant variation (p > 0.05). Therefore, dietary addition of 5 ml/kg JBO promoted fish growth and positively influenced the blood parameters in common carps.  相似文献   

12.
盐度对鲤能量收支的影响   总被引:19,自引:0,他引:19  
邱德依 《水产学报》1995,19(1):35-42
以水蚯蚓为饵料,在温度为27℃,盐度为淡水、3、5、7、9的条件下对幼鲤(初始体重为2.19-3.31g)进行了摄食-生长实验,每一盐度下设4个摄食水平(饥饿-饱足),测定了最大摄食率、吸收率、特定生长率、转化效率和能量收支。结果表明:盐度对最大摄食率、特定生长率(SGR)和转化效率有显著影响;盐度对排出废物所占比例(E/C)影响不显著,对代谢能所占比例(R/C)和生长能所占比例(G/C)有显著影  相似文献   

13.
Jatropha curcas kernel meal is rich in protein; however, it is toxic. Two durations of detoxification process were investigated: 30 and 60 min; designated as Ja and Jb, respectively. Common carp fingerlings (252 fish; 3.2 ± 0.07 g) were fed diets: control containing fishmeal (FM); S50, Ja50 and Jb50: 50% of FM protein replaced by soybean meal (SBM), detoxified Jatropha kernel meal (DJaKM and DJbKM); S75, Ja75 and Jb75: 75% of FM protein replaced by SBM, DJaKM and DJbKM. Highest body mass gain, specific growth rate, metabolic growth rate (MGR) and energy production value were observed for the Jb50 group which were statistically similar to that for control group and significantly (P < 0.05) higher than for all other groups. Lowest feed gain ratio was observed in control group, which was statistically similar to than in Jb50 group. Lowest MGR, protein efficiency ratio, protein productive value and lipid production value were observed in Ja75 group. The dry matter and lipid digestibilities were statistically (P < 0.05) highest in control group and lowest in Ja50 group. Protein and energy digestibilities were statistically similar (P > 0.05) for control and Jb50 groups, and these values were highest among the groups. Highest crude protein efficiency was observed in Jb50 group, whereas lowest in Jb75 group. Relative intestinal length and hepatosomatic index were higher in plant protein–fed groups. Intestinal amylase, protease and lipase activities for control group were statistically higher (P < 0.05) than for plant protein–fed groups. Conclusively, performance of Jb50 group was similar to control group and better than other groups and, thus, is recommended as the most optimal diet for common carp fingerlings.  相似文献   

14.
A three-year test of growth performance andoffspring survival from top-crossing of commoncarp hybrids in low altitude (350 m above thesea level, middle European climate) and in highaltitude (750 m above the sea level, middleEuropean climate) was terminated by assessmentof slaughtering value of edible parts in therespective strains. A recently establishedHungarian synthetic mirror carp strain (HSM)was chosen for testing as maternal strain. TheHSM, as well as wild Amur carp (AC), Ropshacarp (ROP) and Tata carp (TAT) were used aspaternal strains.The evidential strain*altitude interactioneffect was demonstrated as significant forweight of fish (0.0005), fillet without skin(0.0001), index of highbackedness (0.005),index of head length (0.0404),Fulton's coefficient (0.0497),index of widebackedness (0.0315) andgonadosomatic index (0.0082). The interactioneffect was demonstrated as insignificantbetween altitude*sex and betweenstrain*sex.The comparative test of analysis of variance(ANOVA) revealed significantly (P < 0.05)higher weight of fish after killing on lowaltitude (1611.6 g) than on high altitude(1090.3 g), but processed body yield andpercentage of fillet without skin was notsignificantly different (64.0 and 34.2 for lowaltitude, 64.2 and 33.4 for high altitude). Thepercentage of processed body weight andpercentage of fillet without skin in analysisboth altitudes were significantly better infemales (65.5 and 34.6%) than in males (62.5and 33.00%), respectively. The highestsignificant differences in weight of fish afterkilling were found between HSM (890.8 g) andHSM × AC (1283.3 g) in high altitude. In lowaltitude, it was between HSM (1527.2 g) andHSM × AC (1706.9 g) and HSM × ROP (1693.0 g).  相似文献   

15.
The present study was conducted to investigate the effects of fenugreek seed meal (FKSM) on growth performance, blood haematological and biochemical factors, survival and stress resistance of the common carp (2.46 ± 0.06 g) fingerlings. Four practical diets containing 0 (control), 0.5%, 1% and 1.5% FKSM were used to feed fish. Fish were fed on the tested diets at a rate of 3% of body weight for 8 weeks. Results indicated that fish fed the control diet had a significantly lower weight gain, specific growth rate and condition factor compared to the other treatments (P < 0.05). Feed conversion ratio decreased significantly in fish fed diets containing FKSM. There were no significant differences in glucose levels, red blood cell count, haematocrit and haemoglobin between treatment groups and the control group (P > 0.05). Significantly lower white blood cells count was observed in fish treatment with FKSM when compared with the control group (P < 0.05). The cholesterol level in group fed supplemented diet by 1% fenugreek significantly lower than other groups (P < 0.05). Total protein levels were significantly higher in all treatments compared to the control (P < 0.05). No mortality was recorded during the feeding trial. In salinity stress experiment, highest survival rate belong to fish fed supplemented diet by 1% fenugreek. These results indicate that FKSM can be considered as a beneficial dietary supplement for improving the growth performance and blood indices of common carp fingerling.  相似文献   

16.
Abstract
Fanners employing crude human chorionic gonadotropin (Sumach, Infar India Ltd.) for inducing breeding of major carps, in India have claimed that it also promotes growth. The present study was undertaken in order to confirm this and its potential statics pure HCG (Sigma Chemicals Ltd), wherein two experiments were conducted in a recirculatory system. In the first experiment of 84 days duration, 10 mg/kg diet Sumach induced significantly higher growth, but not 20mg/kg diet. Both doses of pure HCG tried (1 and 2 mg/kg diet) were ineffective. In the second experiment, growth produced by 5 mg/kg diet pure HCG and 10 mg/kg diet Sumach were comparable and significantly higher than the rest after 42 days, indicating higher potential of pure HCG. The findings are significant as they reveal that a protein hormone like HCG is capable of growth promotion in carp when administered orally  相似文献   

17.
This study was focused on the clarification of the effect of dietary sesamin on fatty acids and the composition of different lipid fractions [phospholipids (PLs), cholesterol and triacylglycerols] in the white muscle of common carp (Cyprinus carpio L.) juveniles. Two different basic diets with defatted fishmeal as a protein source and either only linseed oil or a mixture of linseed and sunflower oil as a lipid source designed to have two different n‐3/n‐6 ratios (1.21 – CL group; 0.32 – CM group) were produced. Each diet was then used with or without added sesamin (0.58 g 100g?1). One hundred and forty‐four individuals were fed in triplicated groups for 63 days until their weight had doubled. No influence of dietary sesamin on growth, mortality or on the white muscle lipid content of the fish was found. Added sesamin significantly decreased the content of PLs and increased the cholesterol content in the CM group. No effect was found in the total lipid fatty acid composition but there was found a significantly lower content of saturated fatty acids and 20:5n‐3 in PLs and of 22:6n‐3 in triacylglycerols in the sesamin supplemented CL group. These and other differences show either a tendency of lower long chain n‐3 fatty acids biosynthesis or their higher use in β‐oxidation in sesamin‐supplemented groups. We conclude that sesamin in this experiment had no substantial positive impact on the lipid metabolism of juvenile carp.  相似文献   

18.
The effects of cadmium and copper on activity of common carp head kidney hematopoietic tissue were evaluated. The fish were subjected to short-term (3 h, Cd-s and Cu-s) or long-term (4 weeks, Cd-l and Cu-l) exposures to 100 % 96hLC50 or 10 % 96hLC50, respectively. Head kidneys were isolated weekly from 5 fish of each group for 4 weeks (post-short-term exposure and during long-term exposure). Percentage of early blast cells among the hematopoietic precursors was calculated. Proliferative and apoptotic activity were evaluated using immunocytochemical staining for proliferating cell nuclear antigen (PCNA) and caspase 3, respectively. Hematopoietic activity was calculated as the ratio of proliferating to apoptotic cells. All metal exposures induced an increase in frequency of early blast cells. The frequency of proliferating (PCNA-positive) cells also significantly increased. A considerable and significant increase in the frequency of apoptotic cells was the most pronounced effect of metal exposures. Both short-term and long-term treatments caused similar effects, but in case of Cd exposures, the reaction was more pronounced. All metal exposures reduced hematopoietic potential of fish measured as the ratio of proliferating to apoptotic precursor cell frequency. However, in all cases, hematopoietic activity was higher than 1 showing that the rate of repair of hematopoietic tissue prevailed over destruction.  相似文献   

19.
为了探究牛磺酸对脂质氧化饲料饲喂下黄河鲤生长性能和肠道健康的影响,实验先用等量的氧化鱼油(记为OFO)替换基础日粮(记为FO)中的新鲜鱼油,接着将不同含量(0.4%、0.8% 和1.2%)的牛磺酸分别加入OFO组饲料中(分别记为T0.4、T0.8和T1.2),对初始体重为(8.74±0.01)g的黄河鲤进行为期10周的养殖实验。结果显示,与FO组相比,OFO组的终末体质量、增重率、特定增长率和饲料效率均显著降低。上述所有指标在T0.4、T0.8和T1.2组中均显著高于OFO组。另外,OFO组中肝胰脏抗氧化酶的mRNA表达水平显著低于FO组,而牛磺酸添加组中肝胰脏和肠道的抗氧化酶mRNA表达均有不同程度的上调。与FO组相比,OFO组中的肠道消化酶活性,肠绒毛高度、肠绒毛宽度和肌层厚度均显著降低,而在牛磺酸添加组中均得到提高。同样的,OFO组中肠道微生物组成的丰富度和多样性显著降低,条件致病菌丰度明显升高,而有益菌的丰度却有所下降,这些不良现象在牛磺酸组中均得到明显改善。研究表明,牛磺酸可缓解脂质氧化饲料对黄河鲤造成的生长性能抑制、肠道组织结构破坏、消化功能下降及肠道菌群紊乱等不良影响。结合本研究结果,在氧化脂质饲料中牛磺酸的建议添加剂量为0.4~0.8%。本研究为进一步探索牛磺酸对鱼类肠道的生物学功能奠定理论基础。  相似文献   

20.
Cryopreservation of sperm of common carp, Cyprinus carpio L.   总被引:2,自引:0,他引:2  
Milt of common carp, Cyprinus carpio L. was cryopreserved in pellet form with the use of 12 extenders. Most efficient were: BE2 original extender (containing 85 mM NaCl, 50 mM KCl, 3 mm CaCl2, 1 mm MgCl2 with 10% dimethyl-acetamide (DMA) and 10% addition of hen's egg yolk) and Kurokura et al.'s extender with 15% DMA and 10% yolk (about 73% and 69% of eyed eggs, about 61% and 52% of swim-up larvae, respectively). Within the most effective treatments, survival from the eyed-egg stage to the swim-up stage was similar to that observed in the control group. Survival from the eyed-egg stage to the swim-up stage (percentage of eyed eggs was considered as 100%) was highly significantly and positively correlated with the actual rate of swim-up larvae.  相似文献   

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