Forage-Based Feeding in Commercial Red Claw Ponds in Ecuador

Abstract

Red claw crayfish, Cherax quadricarinatus, are perhaps the first tropical species of crayfish to be cultured commercially. Culture traits and high market value could allow semi-intensive culture. Feeding strategies used in extensive crayfish ponds in temperate climates may not be the most appropriate for semi-intensive culture systems where year-round growth is possible. The following study was conducted to evaluate differences between using a pellet-based system versus a pellet-plus-forage-based system. The study was conducted in 14 0.3-ha ponds on a commercial farm in Ecuador. Juvenile red claw (1-2 g) were stocked into all ponds at a rate of 4/m². All ponds received pelleted shrimp rations at a rate of 3% per day. Seven ponds also received dried hay at a rate of 100 kg/ha/mo during the 3-month study. At harvest, red claw weights were similar (30-34 g), but survivals were significantly higher in ponds receiving pellets and hay (65%) than in ponds receiving pellets only (50%). Yields in pellet-fed ponds averaged 594 kg/ha, while red claw receiving pellets plus forage averaged 889 kg/ha. The addition of dried forage was thought to provide a larger variety of natural food organisms throughout a greater area of the pond bottom and provided additional substrate for dispersal and hiding. Addition of grasses from outside the pond is recommended over production of grasses grown within the pond.     

Effects of Stocking Density and Feed on Pond Production Characteristics and Revenue of Harvested Freshwater Prawns Macrobrachiurn rosenbergii Stocked as Size-Graded Juveniles1

A nursery-reared population of juvenile freshwater prawns (Macrobrachium rosenbergii) averaging 0.11 ± 0.058 g was size graded through a 4-mm bar grader producing a numerically 53% upper size population (0.25 ± 0.086 g). These were stocked into triplicate earthen ponds (0.04–0.07 ha each) at densities of 39,540, 59,300 and 79,100/ha, grown for 131 to 134 d, and fed a specially formulated diet. An additional three ponds were stocked at 39,540/ha and fed a commercial sinking catfish feed. Density had a significant effect on average whole body wet weight at harvest but no significant effect on either total yield, survival, or feed conversion. Mean wet weight was significantly higher for prawns stocked at 39,540/ha (34.3 g) than that for those stocked at either 59,300/ha (26.7 g) or 79,100/ha (263 g). The direct relationship between the percentage of small males and increasing density usually seen with ungraded populations was not evidenced in the size-graded populations. Average total yield ranged from 1,041 to 1,662 kg/ha for stocking densities from 39,540 to 79,100/ha. Differences in overall mean wet weight resulted from differences in mean wet weights for orange claw and no claw males and berried and open females, not from differences in morphotype distributions. These differences resulted in significantly higher percentages of tails within the larger count categories and higher revenues for prawns stocked at 39,540/ha. Feed type had no significant effect. Projected net revenues suggest that prawns need to be marketed and sold as a whole product.     

Growth,Processing Measurements,Tail Meat Yield,and Tail Meat Proximate Composition of Male and Female Australian Red Claw Crayfish,Cherax quadricarinatus,Stocked into Earthen Ponds

Abstract

Small (0.7 g) juvenile red claw, Cherax quadricarinatus, were stocked in earthen ponds (0.04 ha) in Kentucky and grown for 86 days so that measures of growth, survival, processing characteristics, and proximate composition of tail muscle in male and female Australian red claw crayfish could be obtained. A commercial marine shrimp was fed to all red in two separate feedings, each consisting of one-half of the total daily ration between 0800-0830 and between 1530-1600 hours for the duration of the culture period. Total weight and number of red claw from each pond were recorded at the harvest. Forty red claw from each sex were randomly sampled, chill-killed by lowering the body temperature in an ice-bath, and frozen (-20°C) for processing measurements and proximate composition. Red claw were removed from the freezer, thawed, individually weighed to the nearest 0.01 g, and then were hand-processed so that weights of chelae, tail, and tail muscle were obtained to the nearest 0.01 g. After 87 days, red claw had a final average individual weight of 59.6 g, weight gain of 8,413%, survival of 64.0%, yield of 903 kg/ha, and feed conversion ratio (FCR) of 4.63. When analyzed by sex, males had significantly (P < 0.05) higher final individual weight (71.05 g) compared to females (53.65 g); higher (P < 0.05) chelae weight (13.1 g for males compared to 6.84 g for females); higher tail weight (25.53 g for males compared to 20.38 g for females); higher tail muscle weight (18.99 g for males compared to 14.89 g for females); and higher cephalothorax weight (32.41 g for males compared to 26.44 g for females; Table 2). There were no significant differences (P > 0.05) in the percentage moisture, protein, lipid, fiber, and ash in the tail muscle of male and female red claw when analyzed either on a wet-weight basis and averaged 81.0%, percentage protein averaged 16.46%, percentage lipid averaged 0.16%, fiber averaged 0.1%, and percentage ash averaged 1.42%. Results from the present study indicate that red claw can be grown as a commercial aquaculture species in locations with limited growing seasons, and that male red claw grow larger, have larger chelae, and higher tail muscle weights compared to females so that it might be economically advantageous to stock all-male populations of red claw in ponds to achieve maximum production. Further research on growth and processing yields of red claw should be conducted to assist the industry.     

Effects on growth,survival, body composition,processing traits and water quality when feeding a diet without vitamin and mineral supplements to Australian red claw crayfish (Cherax quadricarinatus) grown in ponds

To be profitable, producers must reduce diet costs, which can be as high as 80% of the variable costs of an aquaculture expense. As vitamin and mineral premixes represent a significant cost, eliminating addition of these premixes could reduce diet costs if no adverse effects were observed for growth and production parameters. A 105‐day feeding trial was conducted with juvenile Australian red claw crayfish (Cherax quadricarinatus) to evaluate the effects of growth, survival, body composition, processing traits and water quality when red claw were fed a supplemental diet containing 28% crude protein (CP) without vitamin and mineral premixes (and supplemented alfalfa hay) compared with red claw fed a diet (control diet) containing 42% CP, vitamin and mineral premixes, and with supplemented alfalfa hay, and compared with red claw only fed alfalfa hay when grown in ponds. Juvenile red claw (mean weight of 15.7 ± 1.0 g) were randomly stocked into nine 0.02‐ha ponds at a rate of 640 per pond (3.2 per m²), and each treatment was used in three ponds. There were two feedings per day, each consisting of one‐half of the total daily ration. At harvest, individual weight, percentage weight gain, specific growth rate, survival and total yield of red claw fed a control diet was significantly higher (P < 0.05) (83.0 g, 398%, 1.53% day^?1, 65.1%,and 1708 kg ha^?1 respectively) compared with red claw only fed alfalfa hay (44.9 g, 202%, 1.04% day^?1, 30.3% and 431 kg ha^?1, respectively), but not different (P > 0.05) from red claw fed the supplemental diet without vitamin and mineral premixes (76.2 g, 367%, 1.47% day^?1, 57.2% and 1378 kg ha^?1). There were no significant differences (P > 0.05) in feed conversion ratio (FCR) among treatments, which averaged 5.0 (based on prepared diet input). These results indicate that vitamin and mineral supplementation in a diet is not necessary when diet containing 28% CP and alfalfa hay are used in combination for pond grown red claw. These results may help reduce diet costs and possibly increase producers’ profits which might allow for industry expansion.     

Effects of feeding practical diets containing various protein levels on growth, survival, body composition, and processing traits of Australian red claw crayfish (Cherax quadricarinatus) and on pond water quality

A 117‐day feeding trial was conducted in ponds with juvenile Australian red claw crayfish (Cherax quadricarinatus) to evaluate the effects on growth, survival, body composition, and processing traits when fed diets containing three different protein levels (22%, 32%, and 42%), and the effects of feeding these diets on pond water quality. Juvenile crayfish (mean weight of 4.6±2.2 g) were randomly stocked into nine 0.02‐ha ponds at a rate of 500 per pond (25 000 ha^?1), and each diet was fed to three ponds. There were two feedings per day, each consisting of one‐half of the total daily ration. At harvest, there were no significant differences (P>0.05) in the individual weight, percentage weight gain, or specific growth rate among treatments, which averaged 75.3 g, 1535%, and 2.38% day^?1 respectively. Red claw fed the 42% crude protein diet had significantly higher (P<0.05) feed conversion ratio (7.34) compared with crayfish fed diets containing 22% (5.18) or 32% (5.13) crude protein, and had significantly lower percentage survival (46.1%) compared with red claw fed diets with 22% (61.1%) or 32% (58.2%) protein. Total yield was significantly lower (P<0.05) in red claw fed the 42% protein diet (640 kg ha^?1) compared with red claw fed diets containing 22% (920 kg ha^?1) or 32% (904 kg ha^?1) protein. Mean total ammonia nitrogen (TAN) levels were significantly higher (P<0.05) in ponds with red claw fed the 42% protein diet (0.55 mg L^?1) compared with ponds with red claw fed diets containing 22% (0.32 mg L^?1) or 32% (0.38 mg L^?1) protein. Mean total nitrite concentrations in ponds with red claw fed the 42% protein diet was significantly higher (0.05 mg L^?1) compared with red claw fed diets containing 22% (0.01 mg L^?1) or 32% (0.02 mg L^?1) protein. These results indicate that a practical diet containing 22% (as fed basis) protein may be adequate for pond production of red claw when stocked at the density used in this study, and that a diet containing 42% protein adversely affected levels of TAN and nitrite, possibly reducing overall survival of red claw. Use of a diet with 22% protein may allow red claw producers to reduce diet costs and thereby increase profits.     

Production of Australian Red Claw Cherax quudricarinatus in Polyculture with Nile Tilapia Oreochromis niloticus

Abstract— Australian red claw crayfish Cherax quudricarinurus were cultured in monoculture and in polyculture with male Nile tilapia Oreochromis niloricus in nine 0.02-ha earthen ponds. Red claw with an average weight of 7 g were stocked in all ponds at 2/ m². Tilapia with an average weight of 19 g were stocked in six ponds at either 0.5 or 1/m². A sinking 32% fish diet and dry alfalfa hay was provided to all ponds as feed and forage. After 135 d, red claw survival was similar (19–24%) in all treatments while average weights were 76 g in monoculture and 48 g in both polyculture treatments. Lower than expected survival was a result of stress caused by poor shipping conditions. Tilapia survivals were similar (84–90%) in all ponds and had an average weight of 403 g when stocked at 1/m² and 444 g when stocked at 0.5/m². Red claws are generally considered non-aggressive, especially when compared with other clawed crustaceans. This lack of aggressiveness is suggested as a cause for decreased growth in polyculture with tilapia.     

Growth, Survival, and Feed Conversion Rates of Hatchery-Reared Mutton Snapper Lutjanus analis Cultured in Floating Net Cages

Abstract.— The aquaculture performance of mutton snapper Lutjanus analis raised in floating net cages was assessed by measuring their growth, survival, and feed conversion rates during a growout trial conducted in a 3.2‐ha saltwater lake in the Florida Keys, Florida, USA. Approximately 10,500 hatchery‐reared finger‐lings were stocked in two circular, high‐density polyethylene (HDPE) net cages of 7‐m diameter × 7‐m deep (300 m²) and 10‐m diameter × 7‐m deep (600 m³) dimensions. Cages were stocked at 25 fish/m³ (3.2 kg/m³) and 5 fish/m³ (0.72 kg/m³), respectively. Fish grew from a mean of 16.5 g to 302.8 g (25.6 cm TL) in 246 days in the former cage and from a mean of 42.3 g to 245.6 g (23.8 cm TL) in 178 d in the latter cage. Growth rates in weight were best expressed by the following exponential equations: cage 1 (high stocking density): W = 20.716 e^0.0112x (r²= 0.83); cage 2 (low stocking density): W = 38.848 e^0.0118x (r²= 0.81). Length‐weight data indicate that hatcheryraised, cage‐cultured mutton snapper are heavier per unit length than their wild counterparts. There was no significant difference (P < 0.05) between the slopes of the two lines, indicating that fish in the two cages grew at the same rate. The length‐weight relationships for mutton snapper stocked in cages 1 and 2 are expressed, respectively, by the equations W = 0.000009 L ^3.11 (r²= 0.99) and W = 0.000005 L ^3.22 (r²= 0.97). Overall feed conversion rate for both cages combined was 1.4. Approximately 10% of the fish sampled exhibited some degree of deformity, particularly scoliosis. Overall survival rate was 70%. Results suggest that L. analis has potential for aquaculture development in net cage systems.     

Use of Alfalfa Hay, Compared to Feeding Practical Diets Containing Two Protein Levels, on Growth, Survival, Body Composition, and Processing Traits of Australian Red Claw Crayfish, Cherax quadricarinatus, Grown in Ponds

Juvenile red claw, Cherax quadricarinatus, were obtained from a commercial supplier and stocked into twelve 0.04‐ha ponds with an average water depth of approximately 1.1 m at a rate of 1100 red claw per pond (27,170 red claw/ha). Mean stocking weight (±SD) was 6.25 ± 3.0 g. Three replicate ponds were randomly assigned to each of the four treatments. In Treatment 1 (TRT1), red claw were stocked into ponds to which dried alfalfa hay (forage) was added at a rate of 500 kg/ha/mo; Treatment 2 (TRT2) consisted of red claw being fed a prepared diet containing 13% protein; in Treatment 3 (TRT3), red claw were fed a diet containing 13% protein at which had dried alfalfa hay been added at a rate of 500 kg/ha/mo; and in Treatment 4 (TRT4; control), red claw were fed a complete diet containing 28% protein. Water quality measurements were made three times weekly for dissolved oxygen and temperature (am , pm ), pH, total ammonia nitrogen, nitrite, and alkalinity averaging 7.17 mg/L, 8.96 mg/L, 25.5 C, 27.3 C, 8.68, 0.39 mg/L, 0.012 mg/L, and 106.5 mg/L, respectively. After 113 d, the final mean weight of red claw fed TRT3 was significantly (P < 0.05) higher (68.10 g) compared to that of red claw fed hay only (49.40 g) but not different from red claw fed TRT2 (56.03 g) and TRT4 (62.10 g). Likewise, red claw fed TRT3 had significantly higher percent weight gain (990%) compared to that of red claw fed hay only (690%) but not different from TRT2 (796%) and TRT4 (893%). Feed conversion ratio of TRT2, TRT3, and TRT4 and percent survival among all treatments did not differ significantly (P > 0.05), averaging 2.85 and 66.8%, respectively. Total yield of red claw fed TRT3 and TRT4 (968 and 952 kg/ha) was significantly (P < 0.05) higher compared to that of red claw fed hay only (617 kg/ha) but did not differ from red claw fed TRT2 (882 kg/ha). Tail meat yield of male red claw in TRT3 was significantly (P < 0.05) higher (17.3 g) compared to that of male red claw in TRT1 (12.7 g) and TRT2 (14.9 g) but not significantly different (P > 0.05) from male red claw in TRT4 (16.9 g). Tail meat yield of female red claw in TRT3 and TRT4 was significantly (P < 0.05) higher (14.2 and 13.9 g, respectively) compared to that of female red claw in TRT1 (10.5 g) and TRT2 (10.4 g). Tail muscle proximate compositions showed no significant differences (P > 0.05) in protein (wet weight basis) between males and females among treatments (diet) which averaged 16.2 and 14.6%; however, there were little to no significant differences in fat and ash between males and females among treatments. Results from this study indicate that pond‐cultured red claw stocked at 27,170 red claw/ha can be fed a practical diet containing 13% protein, with or without forage (alfalfa hay), compared to red claw fed a complete diet containing 28% protein; however, if alfalfa hay is added to the pond at 500 kg/ha/mo as the sole source of added nutrients, growth is reduced. Therefore, the use of alfalfa hay, in combination with a low‐protein pelleted diet may be a production method for pond‐grown red claw that may reduce costs for producers and thereby increase profits.     

Effect on Growth, Survival, and Fatty Acid Composition of Australian Red Claw Crayfish Cherax quadricarinatus Fed Practical Diets With and Without Supplemental Lecithin and/or Cholesterol

Red claw crayfish Cherux quadricarinatus is one of more than a hundred species of Australian freshwater crayfish. However, because of its rapid growth rate, communal and non‐burrowing behavior, ease of spawning, wide temperature and dissolved oxygen tolerance, and no free‐swimming larval stages, red claw may be the best candidate among the Australian crayfishes for semi‐intensive or intensive aquaculture in the United States. The objective of the study was to examine the effects of growth, survival, and fatty acid composition of newly‐hatched red claw when fed four practical diets with or without lecithin and cholesterol. However, little is known of its nutritional requirements or practical diet formulations. An 8‐wk feeding trial was conducted in a recirculating system with newly‐hatched juvenile (mean individual weight, 0.2 g) red claw, each stocked in separate plastic mesh culture units containing their own individual water line. Water was recirculated through biological and mechanical filters. Practical diets were formulated to be isonitrogenous (40% protein) and isocaloric (4 kcal available enerpy/g of diet) and contained menhaden fish meal (25%), soybean meal (35%, except in Diet 4), and shrimp meal (10%, except in Diet 4) as protein sources. Diet 1 had 0.5% lecithin added and 1.0% cholesterol added: Diet 2 contained 0% lecithin and 1.0% cholesterol; Diet 3 contained 0.5% lecithin and 0% cholesterol: and Diet 4 contained 0% lecithin and 0% cholesterol. After 8 wk, juvenile red claw fed diets with 0% supplemental lecithin (Diets 2 and 4) had no significant difference (P >0.05) in final weight and percentage weight gain (5.6 g and 2626%. respectively) compared to red claw fed the control diet (Diet 1) containing 0.5% lecithin. Red claw fed a diet without added cholesterol (Diet 3) had significantly (P 0.05) lower final weight (3.6 g) and percentage weight gain (1,717%) compared to red claw fed the control diet (Diet 1). However, red claw fed Diet 4 (containing 0% added cholesterol and 0% added lecithin) showed no significant (P >0.05) difference in final weight (5.1 g) and percentage weight gain (2254%) compared to red claw fed all other diets. There was no significant difference (P >0.05) among all diets for specific growth rate (SGR) which averaged 5.38%/d. Percentage survival was not significantly different among all treatments and was 76% for red claw fed Diet 1, 64% (Diet 2), 56% (Diet 3), and 80% (Diet 4). These results indicate that red claw fed Diet 4 containing 25% menhaden fish meal, 44.5% soybean meal, 0.5% choline chloride, 2% cod liver oil and 1% corn oil may satisfy the lecithin and cholesterol requirements and that the addition of dietary lecithin and cholesterol may not be necessary for good growth and survival of small (0.2 g) juvenile red claw. This may allow for less expensive diet formulations for use by producers of red claw crayfish.     

Growth and Survival of the Australian Red Claw Crayfish Cherax quadricarinatus at Three Densities in Earthen Ponds

Australian red claw crayfish Cherax quadricarinatus were cultured in nine 0.02-ha earthen ponds at densities of l/m², 3/m² and 5/m² for 158 d. Average weight at stocking was 3.2 g. All ponds were provided with a combination of hay and corn silage at a rate of 500 kg/ha per month and a commercial crayfish ration fed at 5%, decreasing to 2% of estimated biomass/d during the growing season. Overall survival rate was 72%, and did not differ among treatments. Final yields and average weights varied significantly with stocking density. Red claw averaged 67 g with an average pond yield of 475 kg/ha at l/m². At 3/m² and 5/m², red claw averaged 48 g and 38 g, respectively, and yielded 1,020 kg/ha and 1,422 kg/ha, respectively.     

Production Parameters and Economics of Small-Scale Tilapia Cage Aquaculture in the Volta Lake,Ghana

To calculate the potential for cage aquaculture to create economic opportunities for small-scale investors on the Volta Lake, Ghana, a local NGO with technical support from the Government of Ghana ran two trials (one of four and one of six units) of small-scale cage aquaculture in the town of Dzemeni. Cages were built locally from available materials at a cost of approximately US$1000 per 48 m³ cage. An indigenous line of Nile tilapia, Oreochromis niloticus, was stocked either as mixed sex (first trial) or all-males (second trial) at an average rate of 103 fish/m³ and grown on locally available pelleted feeds for approximately six months. Total costs averaged US$2038 per six-month production cycle. Gross yield ranged from 232 to 1176 kg/cage, averaging 460 kg/cage (9.6 kg/m³). Final average weight of mixed sex populations (253.05 ± 47.43g) was significantly less than of all-males (376.7 ± 72.30g). Likewise, percentage of fish over 300 g at harvest was significantly lower in mixed-sex (38.3%) compared to all-male (75.7%) populations. Mortality resulting primarily from poor handling during transport and stocking averaged 70% and was a major determinate of production and profitability. To break even, harvested biomass of fish needed to exceed 15 kg/m³. At 25 kg/m³, small-scale cage aquaculture generated a net income of US$717 per cage per six months (ROI = 30.2%) on revenues of US$3,500. Water quality in the area surrounding the cages was not negatively affected by aquaculture at the scale tested (5 tons of feed per six months).     

Effect of Intensification on Grow Out of the Amazon River Prawn, Macrobrachium amazonicum

The effects of intensification on growth, survival, productivity, population structure, and distribution of harvested biomass in individual size classes of Macrobrachium amazonicum in semi‐intensive culture were evaluated. Postlarvae (0.01 g) were stocked in 12 ponds at densities of 10, 20, 40, and 80/m² (three replicates per treatment) and raised for 5.5 mo. Average individual weight significantly decreased and productivity significantly increased as stocking density increased (P < 0.001), while survival was not affected (P > 0.05). Prawn mean weight at harvest ranged from 3.6 (80/m²) to 7.0 g (10/m²). Average survival ranged from 65.5% (40/m²) to 72.8% (20/m²), while productivity ranged from 508 (10/m²) to 2051 kg/ha (80/m²). Harvested biomass showed a clear bimodal distribution in individual size classes indicating the occurrence of heterogeneous growth, which may affect management and market strategies. Harvested biomass of prawns weighing more than 7 g (the best market size) increases for stocking densities up to 40/m² and stabilizes between 40 and 80/m². Growth reduction was associated with a decreasing frequency and average weight of green claw 1 and green claw 2 male morphotypes and adult females as density increased. Thus, the distribution of male morphotypes and sexually mature females are affected by density‐dependent factors. Results suggest that prawn density plays an important role on M. amazonicum grow‐out phase, as has been demonstrated for other species of the genus Macrobrachium. M. amazonicum tolerates grow‐out intensification and may be raised in both semi‐intensive and intensive systems stocked at very high densities yielding high productivity.     

Growth, Yield, Dressout, and Net Returns of Bighead Carp Hypophthalmichthys nobilis Stocked at Three Densities in Fertilized Earthen Ponds

Abstract.— Bighead carp Hypophthalmichthys nobilis have been raised in the United States for two decades and sold through the livehaul market, but their profitability in monoculture has not been evaluated. Three studies were conducted in 0.10-ha earthen ponds to evaluate the effect of bighead carp stocking density on growth, yield, dressout yield. and net returns. Initially, bighead carp (average weight of 0.36 kg) were stocked at rates of 500, 320, or 130 fish/ha with three replicates of each treatment. Stocking rates for 2-yr-old fish (average weight of 2.45 kg) were reduced to 320, 220, or 130 fish/ha in the second year. Net yields of bighead carp stocked at 500 fish/ha (963 kg/ha) were significantly higher ( P < 0.05) than net yields at the 320 fishha density (771 kg/ha), and these were significantly greater ( P < 0.05) than net yields at 130 fish/ha (369 kg/ha) in the first growing season. Net yields in the second growing season were not significantly different ( P > 0.05) among densities. There were no significant differences ( P > 0.05) among treatments in yearly growth which ranged from 11–17 g/d in the first and from 6–13 g/d in the second growing season. Dressout percentages for whole-dressed, steak, shank fillet, and shank fillets with white meat only did not differ with stocking density ( P > 0.05). Enterprise and partial budget analysis indicated that monoculture of bighead carp in fertilized ponds is profitable only in the short run at average livehaul market prices, because revenues exceeded variable but not fixed costs. The negative net returns, when all costs were accounted for, indicated that it is not profitable to construct ponds solely for monoculture of bighead carp.     

Supplemental feeds containing coffee pulp for rearing Tilapia in Central AmericaAlimento suplementario conteniendo pulpa de café para la crianza de Tilapia en Centro America

A supplementary feed containing 30% coffee pulp was evaluated for use in the culture of Tilapia aurea in El Salvador, Central America. A comparison of the coffee pulp feed with a feed containing all of the same ingredients except coffee pulp was made with T. aurea raised in 1.0-m³ cages suspended in a fertilized earthen pond. Survival was high in all treatments and there was no significant difference in average weight gain between groups of fish receiving the two experimental feeds. Production trials were conducted in 100-m² fish pens and in 0.05-ha earthen ponds. Pen-raised fish receiving coffee pulp feed grew faster throughout the experiment, and total production was approximately twice that in control treatments. Highest production in pens was 1.25 kg/m² per year. Results of production pond trials using T. aurea at 9 000/ha and stocked with the piscivorous Cichlasoma managuense yielded an estimated 3 392 kg/ha per year in fed treatments and 2 049 kg/ha per year in controls. Low feed conversion (1.92) and low feed cost resulted in an increase in net annual earnings of $251.00/ha.     

Intensification of redclaw crayfish Cherax quadricarinatus culture: II. Growout in a separate cell system

In the process of exploring ways to intensify crayfish culture, a growout system of individual cages (cells) was designed to determine the effects of gender and cell size on the growth of the red claw crayfish Cherax quadricarinatus. Cells of three different diameters—large (25 cm), medium (20 cm) and small (16 cm)—were used. When crayfish were stocked at a mean weight of approximately 10 g, growth rate of males was significantly higher than that of females. The growth rate of the males in the large cells was 0.31±0.14 g/day, while that of the females was 0.18±0.09 g/day. The size of the cell had significant influence on the weight of males. Male crayfish in the large and medium cells grew better than those in the small cells. When males were stocked at a higher mean weight (about 23 g), their mean weight after 206 days was higher in the large cells (69.28±15.72 g) than in the small cells (58.11±12.66 g), suggesting that the growth of large males was also affected by cell size. Regardless of cell size, male animals of this species grew faster than females under conditions of individual cells. This intensive culture method appears to present a powerful improvement in yields, by as much as two orders of magnitude, in comparison with communal cultures.     

Effect of Satiate or Restricted Feeding on Golden Shiner Notemigonus crysoleucas Production in Earthen Ponds

Abstract.— This study was conducted to evaluate the effects of satiate or restricted feeding on the growth and production of golden shiners Notemigonus crysoleucas . Golden shiners (1.2 g) stocked at 560 kg/ha in 12,0.04-ha earthen ponds for 79 d grew to average weights of 5.6, 4.6 and 3.8 g for fish fed to satiation, or 75% or 50% of that amount, respectively. Gross yield ranged from 811 to 1,277 kg/ha; net yield did not differ significantly among treatments.     

Effects of manures and pelleted feeds on survival,growth, and yield of Penaeus stylirostris and Penaeus vannamei in Panama

Brackishwater ponds on the Pacific coast of Panama were stocked at 5/m² with wild-caught postlarval shrimp (0.05 g). Species composition at stocking was 56% Panaeus vannamei, 33% P. stylirostris and 11% P. occidentalis. Experimental treatments were: chicken manure (4500 kg/ha dry weight), cow manure (4500 kg/ha), 25% protein pelleted feed (790 kg/ha) and control (which received no nutrient input). Water was exchanged 5–10% daily throughout the 120-day production period during the 1982 rainy season. Average shrimp yields at harvest, by treatment, were: chicken manure, 262 kg/ha; cow manure, 218 kg/ha; feed, 386 kg/ha; and the control, 160 kg/ha. Average survival for each treatment was 50%, 76%, 58% and 77%, respectively. All P. occidentalis died during the production period. Survival was not significantly different among treatments (P > 0.05). Average weights or shrimp at harvest were 8.72g, 7.32 g, 12.07 g, and 5.98 g, respectively, for the treatments. Manures and feed significantly increased yield over the control (P < 0.002). Feed significantly increased yield over that of the manures (P < 0.0001), while yield for manures did not differ (P > 0.05). A partial budget analysis indicated that a net income for the feed treatment was higher than for chicken manure or cow manure treatments.     

Indoor Spawning and Egg Development of the Red Claw Crayfish Cherax quadricarinatus

In 1991 and 1992, indoor hatchery experiments were conducted with red claw crayfish Cherax quadricarinatus a large tropical crayfish from northern Australia. Six fiberglass tanks were stocked with 40–190 g mature red claw crayfish at 10/m² and a 1:2 male to female ratio. Water temperature was maintained at 28 ± 1 C. Photoperiod was provided by natural daylight in 1991 and controlled lighting in 1992.
With natural lighting, monthly spawning rates (% of females spawning/month) ranged from an average of 15% during months with less than 12 h of light per day to over 35% in months with more than 12 h of light per day. Peak spawning occurred from May to July, too late to obtain juveniles for spring stocking. During the second year, photoperiod was increased from 10L:14D in December to 14L:10D in February and maintained at this level until July. Red claw spawning increased with increasing day length. Peak spawning rates of 50% per month and higher occurred from March to May. Spawning rates decreased dramatically after May, even though photoperiod and temperature remained constant. Eggs per spawn increased with an increase in female size. Fecundity average 7.3 juveniles per gram of body weight for females from 40 to 190 g, regardless of size. Egg color was useful for predicting stage of development.     

Effects of feeding practical diets containing different protein levels, with or without fish meal, on growth, survival, body composition and processing traits of male and female Australian red claw crayfish (Cherax quadricarinatus) grown in ponds

Four practical diets containing 2% of crude protein (CP) (180 and 280 g kg^?1), with or without menhaden fish meal (FM), were fed to Australian red claw Cherax quadricarinatus during a 97‐day feeding trial. Growth, survival, body composition and processing traits of pond‐cultured red claw were determined. Juvenile red claw (mean individual weight of 5.75 ± 3.3 g) were randomly stocked into twelve 0.04‐ha ponds at a rate of 1000 per pond (25 000 ha^?1), and each diet was fed to three ponds. At harvest, the final mean weight of red claw fed Diet 4 (0 g kg^?1 FM and 280 g kg^?1 CP) and Diet 3 (113 g kg^?1 FM and 280 g kg^?1 CP) was significantly (P < 0.05) higher (62.4 and 58.5 g, respectively) than red claw fed Diet 1 (73 g kg^?1 FM and 180 g kg^?1 CP; 51.7 g) and Diet 2 (0 g kg^?1 FM and 180 g kg^?1 CP; 53.0 g). Red claw fed diets containing 280 g kg^?1 CP, with or without FM, had significantly higher percent weight gain (894 and 959%, respectively) compared to red claw fed 180 g kg^?1 CP, with or without FM (778 and 799%, respectively). Feed conversion ratio, percent survival, and total yield among treatments, which averaged 3.55, 65.2%, and 724 kg ha^?1 overall, were not significantly different. Results from this study indicate that pond‐cultured red claw stocked at 25 000 ha^?1 can be fed a practical diet containing 280 g kg^?1 CP with 0 g kg^?1 FM if a combination of plant‐protein ingredients (soybean meal, distillers’ dried grains with solubles, and milo) is added; however, if the percentage of dietary protein level is 180 g kg^?1, growth is reduced even if FM is added at 73 g kg^?1 of the diet. Use of diets containing plant‐protein sources may help reduce diet costs to producers and thereby, increase profits.     

Economic Evaluation of Commercial-Scale, Saltwater Pond Production of Florida Red Tilapia in Puerto Rico

An economic analysis was performed of a proposed commercial-scale 20-ha saltwater pond culture operation for Florida red tilapia in Dorado, northern Puerto Rico. The analysis was based on actual cost and production data from a commercial-scale hatchery, pilot-scale grow-out trials conducted in six 0.2-ha saltwater (avg. = 22.7 ppt) ponds at the Dorado facility and on wholesale market prices ($4.96–5.18/kg) fetched by dressed-out (gilled, gutted and scaled) product. The proposed 20-ha growout facility is comprised of 25 0.8-ha earthen ponds, each supplied with sea water, brackish well water, drainage and aeration, which account for 60.8% of the capital costs. Ponds are stocked with fingerlings (0.85 g avg. wt.) at a density of 3.0 fish/m² (30,000/ha), and are harvested at 160 and 220 d, at an average weight of 545 g for a total yield of 11,445 kg/ha per crop. Imported feed ($0.55/kg), processing and distribution ($0.50/kg) and sex-reversed fry ($0.11/fry) are the highest variable costs, accounting for 30.7%, 15.4% and 13.9%, respectively, of the total annual costs. Salaries and benefits, and depreciation represent the highest fixed costs, accounting for 8.4% and 5.5%, respectively, of the total annual costs. Under these conditions, a wholesale price of $4.55/kg results in a positive cash flow by year eight, and a breakeven price, internal rate of return (IRR), net present value (NPV) and discounted payback period (DPP) of $4.08/kg, 7.6%, ($235,717) and >10 yr, respectively, suggesting that the proposed 20-ha operation is not economically feasible under these conditions. The proposed enterprise is marginally feasible if stocking density is increased to 3.5 fish/m² while at 4.0 fish/m² economic outlook is favorable. Costs can be lowered considerably by targeting production and market variables most sensitive to profitability indices, using locally-prepared feeds, and vertically integrating hatchery and growout operations.