保护性耕作对土壤微团聚体碳、氮分布的影响

以7年不同耕作的定位试验为研究对象,研究了深松、旋耕、免耕等保护性耕作对关中土小麦-玉米轮作条件下微团聚体分布以及微团聚体中有机碳和全氮含量的影响。结果表明,秸秆粉碎旋耕、秸秆覆盖深松处理提高了特征微团聚体的组成比例,而秸秆还田+传统耕作和免耕处理对特征微团聚组成比例的影响较小。与传统耕作比较,旋耕、深松处理均能提高较大粒级微团聚体中(0.050~.25 mm和0.010~.05 mm)有机碳及全氮含量,而免耕使0.010~.05 mm微团聚体中的有机碳和全氮含量有所降低;旋耕、深松和免耕对小粒级微团聚体(0.01 mm)中的有机碳和全氮含量影响较小。相关分析表明,土壤有机碳与0.050~.25 mm和0.010~.05 mm粒级微团聚体中的有机碳、全氮之间均显著正相关,而与0.01 mm粒级微团聚体的有机碳、全氮含量不相关;土壤全氮与0.05~0.25 mm粒级的全氮、有机碳显著相关。0.010~.05 mm粒级微团聚体的碳、氮与0.050~.25 mm粒级微团聚体的碳、氮间显著正相关,而0.01 mm粒级微团聚体的碳、氮与其它粒级的碳、氮间不相关。从土壤微团聚体中碳、氮的分布考虑,旋耕、深松是当地较理想的耕作方式。     

稻田垄作免耕对土壤真菌多样性分布的影响

在水田长期垄作免耕处理下,研究了土壤真菌多样性及其在不同大小团聚体中的分布.结果表明,垄作免耕处理下,耕层土壤中,真菌主要分布于粒级为1.0～0.25 mm的土壤团聚体中,同时在＞0.053 mm的各级土壤团聚体中垄作免耕处理下真菌数量多于常规轮作处理;垄作免耕处理的各级土壤团聚体中都有毛霉和根霉存在,且1.0～0.25mm土壤团聚体中属类最丰富,其simpson多样性指数为0.93,Shannon多样性指数为1.24,常规轮作下属类丰度没有明显变化.稻田垄作免耕提高了不同团聚体中真菌的数量、多样性,改变了真菌在不同大小团聚体中的分布状况.     

不同肥力红壤水稻土根际团聚体组成和碳氮分布动态

研究水稻种植期间表层土壤团聚体数量及其有机碳、全氮含量的变化,对揭示人为耕作的影响、认知土壤肥力的演变规律具有重要意义。选择两种不同肥力的红壤性水稻土进行田间根袋试验,分别于水稻插秧前、分蘖期、孕穗期和成熟期采样,分析了水稻生长过程中根际和非根际土壤团聚体组成、稳定性以及有机碳、全氮分布的动态变化。结果表明,低肥力土壤团聚体以0.25 mm大团聚体为主(56.2%~64.0%),0.25~1 mm粒级团聚体含量最高;除1~2 mm粒级外,水稻生育期内根际土壤各粒级团聚体含量均有显著变化;取样时期、根际作用与取样时期的交互效应对0.25~1 mm和0.053~0.25 mm粒级含量有显著影响。高肥力土壤中以0.25 mm微团聚体为主(59.8%~72.0%),0.053~0.25 mm粒级团聚体比例最高,取样时期显著影响0.25 mm大团聚体含量,根际作用与取样时期的交互效应对2 mm粒级含量有极显著影响。与非根际相比,根际土壤大团聚体的破坏率较低,平均重量直径(MWD)较高,种植水稻有助于提高根际土壤的稳定性。两种肥力土壤团聚体中有机碳和全氮含量均表现为1~2 mm粒级最高,0.053~0.25 mm粒级最低,大团聚体中显著高于微团聚体。根际土壤中,水稻成熟期各粒级团聚体有机碳含量与插秧前无显著差异,而分蘖期和孕穗期有明显波动;水稻的生长降低了大团聚体中的全氮含量,对高肥力土壤的影响更为显著。总体而言,低肥力土壤中,根际作用主要影响团聚体组成和稳定性,取样时期影响团聚体碳氮含量;高肥力土壤中,团聚体组成和碳氮分布受根际作用和取样时期的共同影响。     

氮素补充对高寒草甸土壤团聚体有机碳、全氮分布的影响

土壤结构的稳定性对高寒草甸生态系统有重要意义。为研究不同水平氮素补充对高寒草甸草地土壤团聚体、有机碳和全氮含量及分布格局的影响,于2012-2014年在青藏高原东部夏河县进行3 a的试验研究。试验为随机区组设计,包括0(对照)、50(低氮)、100(中氮)和200(高氮)kg/hm2 4个氮素补充水平。研究结果表明:低、中、高水平氮素补充处理显著提高了0~30 cm土层≥0.25 mm大团聚体质量分数(P0.05),比对照分别提高了4.74%、6.42%和1.96%;较之对照,低、中水平氮素补充处理显著增加了≥5 mm、≥2~5 mm粒级团聚体含量;低、中水平氮素补充处理显著提升了0~30 cm土壤团聚体平均质量直径,分别比对照提升了9.79%和12.63%。不同水平氮素补充处理有机碳、全氮含量大小排序分别为:中氮≈低氮对照高氮、中氮低氮≈高氮对照。不同粒级团聚体中0.25 mm微团聚体有机碳含量最高而全氮含量最低、≥0.25~2 mm粒级有机碳含量最低而全氮含量最高;低、中水平氮素补充提高了不同粒级团聚体0~30 cm土层有机碳含量而高水平氮素补充处理显著降低了有机碳含量;低、中、高水平氮素补充增加了不同粒级团聚体全氮含量,其中中水平氮素补充处理最高,低水平氮补充次之。不同粒级团聚体含量是影响团聚体养分贡献率的主要原因,≥2~5 mm粒级团聚体含量与相应粒级团聚体有机碳含量呈显著正相关关系,≥5 mm和≥2~5 mm粒级团聚体含量与相应粒级团聚体全氮含量分别呈极显著正相关、显著正相关关系。研究表明连续每年补充50~100 kg/hm2氮可以改善高寒草甸土壤结构并提高土壤肥力状况。     

耕作方式对土壤不同粒径团聚体氮素矿化的影响

研究了常规耕作和垄作免耕两种耕作方式下紫色水稻土不同粒径团聚体中氮的矿化过程。结果表明,不同粒径团聚体氮素矿化强度具有显著差异:潜在矿化势(Np)的最高值均出现在2.0～0.25 mm大团聚体中,最低值在>2.0 mm的宏团聚体中;2.0～0.25 mm团聚体的潜在矿化速率(Vp)和实际矿化速率(Va)比其他团聚体高1.2～1.6倍。常规耕作和垄作免耕两种耕作方式下不同团聚体的净矿化率随时间的变化趋势相同;垄作免耕显著增加了氮素的净矿化量,以原土为例:垄作免耕处理Np比常规耕作处理高33.9%,Vp和Va分别比常规耕作处理高85.2%和52.0%。虽然垄作免耕可以显著促进土壤中氮素的矿化作用,但是耕作方式对不同粒径团聚体内的氮矿化动力学过程并无显著影响,表明耕作方式对不同大小团聚体中可矿化氮和微生物的分布影响较小。     

生物炭对豫西丘陵区农田土壤团聚体稳定性及碳、氮分布的影响

为研究生物炭对豫西丘陵地区农田土壤团聚体分布、稳定性及其碳、氮在团聚体中分布的影响,进一步探明生物炭对丘陵区农田土壤结构和养分的长期作用效果。采用田间长期定位试验,生物炭用量为0(C0),20(C20),40(C40)t/hm~2 3个处理,研究生物炭施用5年后对土壤团聚体组成及稳定性的影响,探究土壤团聚体中有机碳和全氮分布特性。结果表明:施加20,40 t/hm~2生物炭可提高0—20,20—40 cm土层的机械性0.5 mm以上粒级和水稳性0.053 mm以上粒级团聚体含量。在0—20 cm土层中,C20和C40处理下0.25 mm的机械性团聚体(DR_(0.25))分别较对照增加3.78%和6.83%,0.25 mm水稳性团聚体(WR_(0.25))分别较对照增加31.0%和49.45%,土壤不稳定团粒指数(E_(LT))分别较对照降低4.30%和6.85%,土壤团聚体破坏率(PAD)分别较对照降低9.71%和14.77%,土壤团聚体平均质量直径(MWD)分别较对照增加28.44%和45.34%,几何平均直径(GMD)分别较对照增加32.04%和54.92%。各粒级的有机碳和全氮含量随生物炭施用量的增加而增加,有机碳和全氮含量都以0.25～0.053 mm粒级最高,且0—20 cm土层的有机碳和全氮含量高于20—40 cm土层的有机碳和全氮含量;随着生物炭施用量的增加,2,2～0.25,0.25～0.053 mm粒级团聚体有机碳和全氮贡献率随之增加,而0.053 mm粒级微团聚体有机碳和全氮贡献率随之降低。总体来说,生物炭能够改善豫西丘陵地区农田土壤的团聚体结构,增加土壤大团聚体的含量,增强团聚体的稳定性,提高土壤团聚体中碳、氮含量,有利于豫西地区农田土壤肥力的保持和持续健康发展。     

添加玉米秸秆对黑土团聚体碳氮分布的影响

通过室内模拟实验,研究了黑土添加玉米秸秆对团聚体组成、有机碳、氮及净积累有机碳、氮在不同粒径团聚体中的分布和碳、氮贮量的影响,探讨不同粒径团聚体对土壤固碳和肥力的贡献份额.研究结果表明:未添加玉米秸秆黑土,0.25～0.053 mm微团聚体含量最多,>2 mm大团聚体最少;土壤有机碳、氮主要分布在>2 mm和2～0.25 mm团聚体中;2～0.25 mm和0.25～0.053 mm团聚体中土壤有机碳、氮贮量最高.黑土添加玉米秸秆360 d期间,促进了土壤的团聚作用,>2 mm大团聚体成为优势粒级;土壤有机碳和净积累有机碳主要分布在>2 mm团聚体中,0.25～0.053 mm团聚体中分布最少;全氯和净积累氮主要分布在>2 mm和<0.053 mm团聚体中;土壤有机碳、氮贮量随着团聚体粒径的增大而增加.     

不同植被覆盖对养分在土壤水稳性团聚体中分布特征的影响

研究了重庆缙云山不同植被覆盖下土壤结构的稳定性,以及土壤有机碳和养分在水稳性团聚体中的分布特征.结果表明:不同植被覆盖对土壤水稳性团聚体的分布和土壤结构稳定性有显著影响.竹林土壤>0.25 mm水稳性团聚体含量以及土壤结构的稳定性显著低于马尾松林和草地土壤.植被覆盖对养分在土壤水稳性团聚体中的分布模式没有显著影响,在>2mm水稳性团聚体和<0.053 mm粒级的粉砂与粘粒组分中,有机碳、全氮、全磷以及交换性K~+、Na~+、Ca~(2+)、Mg~(2+)的浓度最高;地表植被覆盖的变化对有机碳、全氮、全磷和交换性盐基离子在各粒级水稳性团聚体中的含量产生显著影响,草地和竹林土壤有机碳和全氮显著高于马尾松林土壤,主要表现在0.25-0.053 mm粒级水稳性团聚体中有机碳和全氮含量,草地和竹林土壤显著高于马尾松林土壤;而草地土壤>2 mm水稳性团聚体和<0.053 mm粒级的粉砂与粘粒组分中的磷显著高于马尾松林和草地土壤.不同植被覆盖下土壤水稳性团聚体中交换性盐基离子均以Ca~(2+)、Mg~(2+)为主,占交换性盐基总量(Total exchangeable bases TEB)的91.8%～92.9%.草地土壤各个粒级的TEB都要大于其他两种植被覆盖下的土壤.     

有机物料对稻田土壤团聚体及有机碳分布的影响

通过连续5年定位试验,以紫云英、秸秆和商品有机肥等有机物料还田的稻田土壤为对象,研究有机物料还田后不同物理分组下土壤组成特点和有机碳变化特征。结果表明,稻田土壤团聚体主要分布在2~0.25mm与0.25~0.053mm粒级,团聚体颗粒有机碳含量随着粒径的减小而减少。有机物料还田可提高0.25~0.053mm和0.053mm粒级团聚体有机碳的含量,紫云英、秸秆、商品有机肥等有机物料可通过提高土壤微团聚体有机碳含量而增加土壤碳库。有机物料施用增加土壤轻组组分颗粒含量,减少重组组分颗粒含量,有助于土壤轻组组分的形成。稻田土壤轻组颗粒有机碳含量与0.25mm和0.053mm团聚体颗粒有机碳含量呈显著相关,与2~0.25mm团聚体颗粒有机碳含量呈极显著相关。稻田土壤施用紫云英、秸秆和商品有机肥等有机物料,可有效提高土壤微团聚体和轻组成分颗粒含量,增加土壤微团聚体和轻组有机碳含量,改变稻田土壤有机碳库组成特征。     

轮作对黑土团聚体有机质光谱特征的影响

为研究作物轮作对黑土各粒级团聚体碳氮含量及红外光谱结构特征的影响,选取中国科学院海伦农业生态试验站长期定位试验中大豆连作(SC)、大豆轮作(当季为大豆,SR)、玉米连作(MC)、玉米轮作(当季为玉米,MR)下的土壤为样品,用湿筛法得到大团聚体(0.25mm)、微团聚体(0.25~0.05mm)和粉黏粒(0.05mm)3个粒级,并测定其碳、氮含量和红外光谱。结果表明,土壤中以大团聚体为主。轮作对各粒级团聚体含量没有影响,轮作下原土、大团聚体和微团聚体的碳、氮含量高于连作,且玉米轮作与玉米连作之间差异显著;3个粒级团聚体碳氮含量差异显著,且微团聚体中含量最高。轮作和连作下3个粒级团聚体中官能团类型一样,含量大小均为多糖中的C—O和无机物中的Si—O碳水化合物的—OH或—NH芳香族C=C自由—OH脂肪族C—H,且轮作下脂肪族、芳香族和含烷氧结构的物质高于连作,在微团聚体中表现明显,表明轮作能促进土壤稳定性结构形成,增加稳定性物质含量,有利于碳氮固定于微团聚体中,稳定并增加土壤有机质。     

生物炭施用下潮土团聚体微生物量碳氮和酶活性的分布特征

[目的]探究生物炭配施化肥对不同粒级团聚体中微生物量碳、氮(MBC、MBN)含量和胞外酶活性的影响,分析影响团聚体胞外酶活性变化的主控因素,为提升土壤质量提供科学依据.[方法]田间微区试验在河南现代农业研究基地进行,供试土壤为石灰性潮土.设置4个处理:不施肥(CK)、单施化肥(NPK)、单施生物炭(BC)和生物炭配施化...     

Contributions of soil biota to C sequestration varied with aggregate fractions under different tillage systems

It is increasingly believed that substantial soil organic carbon (SOC) can be sequestered in conservation tillage system by manipulating the functional groups of soil biota. Soil aggregates of different size provide diverse microhabitats for soil biota and consequently influence C sequestration. Our objective was to evaluate the contributions of soil biota induced by tillage systems to C sequestration among different aggregate size fractions. Soil microbial and nematode communities were examined within four aggregate fractions: large macroaggregates (>2 mm), macroaggregates (2–1 mm), small macroaggregates (1–0.25 mm) and microaggregates (<0.25 mm) isolated from three tillage systems: no tillage (NT), ridge tillage (RT) and conventional tillage (CT) in Northeast China. Soil microbial and nematode communities varied across both tillage systems and aggregate fractions. The activity and abundance of microbes and nematodes were generally higher under NT and RT than under CT. Among the four aggregate fractions, soil microbial biomass and diversity were higher in microaggregates, while soil nematode abundance and diversity were higher in large macroaggregates. Structural equation modelling (SEM) revealed that the linkage between microbial and nematode communities and their contributions to soil C accumulation in >1 mm aggregate fractions were different from those in <1 mm aggregate fractions. Higher abundance of arbuscular mycorrhizal fungi (AMF) could enhance C retention within >1 mm aggregates, while more gram-positive bacteria and plant-parasitic nematodes might increase C accumulation within <1 mm aggregates. Our findings suggested that the increase in microbial biomass and nematode abundance and the alteration in their community composition at the micro-niche within aggregates could contribute to the higher C sequestration in conservation tillage systems (NT and RT).     

Long-term tillage effects on the distribution patterns of microbial biomass and activities within soil aggregates

The effects of tillage on the interaction between soil structure and microbial biomass vary spatially and temporally for different soil types and cropping systems. We assessed the relationship between soil structure induced by tillage and soil microbial activity at the level of soil aggregates. To this aim, organic C (OC), microbial biomass C (MBC) and soil respiration were measured in water-stable aggregates (WSA) of different sizes from a subtropical rice soil under two tillage systems: conventional tillage (CT) and a combination of ridge with no-tillage (RNT). Soil (0–20 cm) was fractionated into six different aggregate sizes (> 4.76, 4.76–2.0, 2.0–1.0, 1.0–0.25, 0.25–0.053, and < 0.053 mm in diameter). Soil OC, MBC, respiration rate, and metabolic quotient were heterogeneously distributed among soil aggregates while the patterns of aggregate-size distribution were similar among properties, regardless of tillage system. The content of OC within WSA followed the sequence: medium-aggregates (1.0–0.25 mm and 1.0–2.0 mm) > macro-aggregates (4.76–2.0 mm) > micro-aggregates (0.25–0.053 mm) > large aggregates (> 4.76 mm) > silt + clay fractions (< 0.053 mm). The highest levels of MBC were associated with the 1.0–2.0 mm aggregate size class. Significant differences in respiration rates were also observed among different sizes of WSA, and the highest respiration rate was associated with 1.0–2.0 mm aggregates. The C_mic/C_org was greatest for the large-macroaggregates regardless of tillage regimes. This ratio decreased with aggregate size to 1.0–0.25 mm. Soil metabolic quotient (qCO₂) ranged from 3.6 to 17.7 mg CO₂ g^− 1 MBC h^− 1. The distribution pattern of soil microbial biomass and activity was governed by aggregate size, whereas the tillage effect was not significant at the aggregate scale. Tillage regimes that contribute to greater aggregation, such as RNT, also improved soil microbial activity. Soil OC, MBC and respiration rate were at their highest levels for 1.0–2.0 mm aggregates, suggesting a higher biological activity at this aggregate size for the present ecosystem.     

Soil organic carbon and nutrient content in aggregate‐size fractions of a subtropical rice soil under variable tillage

The effects of tillage on soil organic carbon (SOC) and nutrient content of soil aggregates can vary spatially and temporally, and for different soil types and cropping systems. We assessed SOC and nutrient levels within water‐stable aggregates in ridges with no tillage (RNT) and also under conventional tillage (CT) for a subtropical rice soil in order to determine relationships between tillage, cation concentrations and soil organic matter. Surface soil (0–15 cm) was fractionated into aggregate sizes (>4.76 mm, 4.76–2.00 mm, 2.00–1.00 mm, 1.00–0.25 mm, 0.25–0.053 mm, <0.053 mm) under two tillage regimes. Tillage significantly reduced the proportion of macroaggregate fractions (>2.00 mm) and thus aggregate stability was reduced by 35% compared with RNT, indicating that tillage practices led to soil structural change for this subtropical soil. The patterns in SOC, total N, exchangeable Ca²⁺, Mg²⁺ and total exchangeable bases (TEB) were similar between tillage regimes, but concentrations were significantly higher under RNT than CT. This suggests that RNT in subtropical rice soils may be a better way to enhance soil productivity and improve soil C sequestration potential than CT. The highest SOC was in the 1.00–0.25 mm fraction (35.7 and 30.4 mg/kg for RNT and CT, respectively), while the lowest SOC was in microaggregate (<0.025 mm) and silt + clay (<0.053 mm) fractions (19.5 and 15.7 mg/kg for RNT and CT, respectively). Tillage did not influence the patterns in SOC across aggregates but did change the aggregate‐size distribution, indicating that tillage affected soil fertility primarily by changing soil structure.     

Bacterial diversity in water-stable aggregates of soils under conventional and zero tillage management

Reduced tillage of agricultural soils has been shown to result in greater macroaggregation, microbial biomass and microbial diversity. While it has been shown that macroaggregates contain more microbial biomass per unit soil mass than microaggregates, it is unclear how microbial diversity varies with soil aggregation. We investigated the functional diversity (catabolic potential) of bacteria, evaluated by calculating Shannon’s diversity index (H′), substrate richness (S) and substrate evenness (E) from potential substrate utilization patterns, in whole soil (i.e. not separated into different aggregate sizes) and aggregates of different sizes (2–4, 1–2, 0.5–1, 0.25–0.5, and 0.1–0.25 mm diameter) in loam and silt loam soils grown to barley and managed for 6 years under conventional tillage (CT) or zero tillage (ZT) systems in northern British Columbia. There were no significant tillage effects on bacterial diversity in whole soils. In soil aggregates, H′ and E were significantly higher under CT than under ZT on the loam at barley planting time, with no significant aggregate size effects. However, at barley-heading stage, all diversity indices in both soils were significantly higher under ZT than under CT, and they tended to increase with increasing aggregate size. Cluster analysis and principal component analysis of substrate utilization patterns also revealed differences in bacterial community structures between CT and ZT, but the substrates that were utilized differently between the two tillage systems were not the same between soil types or sampling times. The results during the cropping cycle imply that deterioration of soil structure is probably one factor that explains the adverse effects of soil tillage on soil microbial biomass and diversity.     

秸秆还田对盐渍土团聚体稳定性及碳氮含量的影响

以黄河三角洲典型盐化潮土为研究对象,分析了3种盐渍化程度(轻度、中度、重度)和3 a连续秸秆还田下土壤水稳性团聚体组成、稳定性以及各级团聚体C、N含量的变化。研究结果表明:重度盐渍土0.25～2 mm和0.053～0.25 mm团聚体所占比例显著低于轻度和中度盐渍土;土壤盐分含量与0.25～2mm团聚体中有机碳和全氮的分配比例、0.053～0.25 mm团聚体中全氮的分配比例成显著负相关。秸秆还田使轻度盐渍土平均重量直径(MWD)、几何平均直径(GMD)和0.25 mm团聚体所占比例(R0.25)分别增加47.6%、39.7%和54.0%,使中度盐渍土MWD、GMD和R0.25分别增加31.0%、31.9%和31.4%;各粒级中秸秆还田使轻度盐渍土0.053～0.25 mm粒级有机碳和全氮含量增加最多,增加比例分别为29.1%和28.8%,该粒级中C、N分配比例也显著提高;秸秆还田使中度盐渍土0.25～2 mm团聚体有机碳及其分配比例提高最多,比例分别为56.1%和58.7%。秸秆还田对轻度和中度盐渍土团聚体的稳定性均起到了明显的改善作用,但不同盐渍土秸秆还田对土壤团聚体C、N分布的影响明显不同。     

不同耕作方式对土壤有机碳、微生物量及酶活性的影响

【目的】依托8年长期(2005~2012)固定道定位试验,研究不同耕作方式对土壤有机碳、土壤微生物量、土壤酶活性在0—90 cm土层的分布特征,为优化中国西北干旱区的耕作方式提供理论依据。【方法】试验包括固定道垄作(PRB)、固定道平作(PFT)与传统耕作(CT)三种耕作模式下的土壤有机碳土壤总有机碳(TOC)、颗粒有机碳(POC)、土壤微生物量碳(MBC)、土壤微生物量氮(MBN)、土壤微生物量磷(MBP)、蔗糖酶、过氧化氢酶、脲酶及小麦产量进行了测定和分析。【结果】在0—90 cm土层,不同耕作方式下的TOC、POC、MBC、MBN、MBP、蔗糖酶活性、脲酶活性均随着土层的增加呈下降趋势,过氧化氢酶活性呈先下降后增大的分布特征;在0—60 cm,固定道保护性耕作能够显著增加心土层作物生长带土壤有机碳储量,有机碳储量大小为PRBPFTCT;PRB、PFT较CT可以显著增加0—10 cm作物生长带TOC、POC、MBC、MBN、MBP含量、蔗糖酶、脲酶活性,其大小为PRBPFTCT;耕作方式对过氧化氢酶活性影响不显著;TOC、POC、MBC、MBN、MBP、蔗糖酶活性、脲酶活性、过氧化氢酶活性之间均达到了显著或极显著相关。【结论】PRB较PFT、CT能够提高耕作层(0—10 cm)土壤有机碳含量、土壤微生物量、土壤酶活性, 增加作物产量, 增大0—60 cm土层有机碳储量,耕作方式(PRB、PFT及CT)对10 cm以下土层土壤环境改善作用不明显。     

玉米间作大豆、萝卜对红壤不同粒径水稳性团聚体碳氮分布的影响

为了探讨不同种植模式对红壤水稳性团聚体粒径分布以及不同粒级团聚体中有机碳、氮含量,土壤微生物量碳、氮含量的影响,选择大豆、萝卜与玉米间作及3种作物的单作进行田间试验,在作物成熟期采样,进行样品测定。结果表明:在玉米-大豆(玉//豆)、玉米-萝卜(玉//萝)、玉米单作(玉单)、大豆单作(豆单)、萝卜单作(萝单)处理中,大团聚体(> 0.25 mm)的百分含量明显高于微团聚体(<0.25 mm),其中以大团聚体中粒径为0.25~2 mm的百分含量最高且显著高于其余两种粒径,大团聚体百分含量表现为:玉//豆>玉//萝>玉单>萝单>豆单,微团聚体则与其相反。团聚体粒径中的有机碳含量表现为0.25~2 mm粒径最高、<0.25 mm粒径次之、>2 mm粒径含量最少、玉米大豆间作含量最高、玉米单作含量最低。团聚体粒级中全氮含量与有机碳分布一致,但单作的全氮含量高于间作。有机碳与全氮的C/N为间作高于单作,>2 mm粒径C/N高于其余两种粒径。微生物量碳、氮在团聚体粒径中的分布趋势一致,都表现为间作模式高于单作模式,大团聚体中的含量高于微团聚体。微生物量碳、氮的C/N为单作模式高于间作模式,微团聚体大于大团聚体。与单作相比,间作能提高土壤中水稳性大团聚体的含量,增加大团聚体粒径内的有机碳、氮及微生物量碳、氮的含量。     

保护性耕作下土壤团聚体组成及其有机碳分布特征

依据吉林省德惠市田间定位试验（始于2001年）,对玉米-大豆轮作和玉米连作模式下秋翻（MP）、垄作（RT）和免耕（NT）3种耕作方式的机械稳定性团聚体和水稳性团聚体粒级分布、水稳性团聚体有机碳含量及团聚体稳定性进行了研究。结果表明,3种耕作方式下,〉0.25 mm机械稳定性团聚体含量均在70%以上,最高可达93.29%,各粒级含量在两个土层中表现规律性不强。水稳性团聚体含量均在20%以上,最高可达35.5%,且表层高于底层。与干筛法测定的团聚体相比,〉0.25 mm团聚体含量明显减少,最大减少幅度为58.76%。两个土层中玉米-大豆轮作和玉米连作下的机械稳定性团聚体与水稳性团聚体对耕作处理的响应表现出一定的相似性,即RT〉NT〉MP。水稳性团聚体有机碳含量随粒径的减小而增大,3种耕作方式下有机碳含量表现为NT〉RT〉MP,表层高于底层,且玉米-大豆轮作高于玉米连作。比较3种耕作方式,垄作更有利于团聚体的形成和稳定,且玉米-大豆轮作好于玉米连作。