金丝桃素的体外抗口蹄疫病毒活性

将体外培养的BHK21细胞感染FMDV后加入金丝桃素，日光灯下光活化1h，通过细胞病变观察、MTT法、透射电子显微镜观察、H^3-UR掺入试验，探讨了光活化金丝桃素对FMDV体外生长增殖的影响。结果表明，金丝桃素有明显的抑制FMDV致BHK21细胞病变效应，能抑制FMDV的增殖，抑制率可达61．82％。透射电镜观察，金丝桃素组与病毒对照组相比，BHK21细胞的FMDV颗粒明显减少。证实，金丝桃素在体外有明显的抑制FMDV增殖的作用。     

NLRP3炎症小体对口蹄疫病毒的抑制及其活化机理

口蹄疫(Foot and mouth disease,FMD)是由口蹄疫病毒(Foot and mouth disease virus,FMDV)感染偶蹄动物引起的一种急性、高热、接触性传染病。FMD被誉为畜牧业的“头号杀手”。动物感染FMDV后,会产生明显的炎症反应,引起水疱、高热等临床症状,但FMDV刺激产生炎症反应的分子机制尚未明确。天然免疫系统是宿主抵抗病毒入侵的第一道防线。     

注射口蹄疫灭活苗引起家畜过敏反应与救治

口蹄疫是由口蹄疫病毒引起的偶蹄动物的急性热性高度接触性传染病。目前,大多数国家都把疫苗接种作为防制的关键措施之一。但预防注苗过程中偶尔会发生过敏反应,据海晏县3年注苗过敏反应情况调查,发病率为0.15%~0.32%。不及时救治极易引起死亡,不但给群众造成经济损失,而且给预     

口蹄疫防控与研究动态

口蹄疫（foot and mouth disease，FMD）由口蹄疫病毒引起偶蹄动物共患的急性、热性、高度接触性传染病。也是造成家畜及其产品国际贸易障碍的重要疾病，被国际兽医局（OIE）列为A类传染病之首。近年又被列为重大跨国动物疫病的全球消灭行动计划及生物武器安全公约组织重点检查对象。     

光与氧对金丝桃素蛋白复合物抗口蹄疫病毒的活性的影响

口蹄疫是一种由病毒(口蹄疫病毒(foot-and-mouth disease virus,FMDV)属于小RNA病毒科FMDV属,完整病毒含有单股正链RNA,衣壳蛋白,无包膜)感染引起的偶蹄动物,如牛、羊、猪、骆驼、鹿等共患的急性接触性传染病。易感染口蹄疫的偶蹄动物约有70多种,马不会感染口蹄疫,但会成为口蹄     

口蹄疫病毒非结构蛋白3C真核表达载体的构建及表达

口蹄疫(FMD)是由口蹄疫病毒(FMDV)引起,属于小RNA病毒科口蹄疫病毒属[1 2],为单链正股RNA[3]。T racey认为3C蛋白酶具有病毒加工、宿主蛋白裂解的作用[4]。H ata认为在感染FMDV的宿主细胞内翻译的一条多肽链,11个蛋白酶裂解位点中,9个位点由3C蛋白酶完成裂解过程[5]。目前,对同     

口蹄疫的诊断与防治

口蹄疫 (foot and mouth disease,FMD),是口蹄疫病毒(FMDV)引起的偶蹄动物多发的一种急性、热性和传播极为迅速的接触性传染病.偶见于人和其他动物.其特征为嘴周围、口内、蹄、乳头和乳房上形成水疱.     

猪口蹄疫的特点与防治

1 口蹄疫病毒特征 口蹄疫是由口蹄疫病毒（FMDV）感染引起的偶蹄动物共患的急性、热性、高度接触性传染病,最易感染的动物是牛、猪、骆驼、羊、鹿等,野猪、野牛等野生动物也易感染此病。本病以牛最易感,羊的感染率较低。口蹄疫在亚洲、非洲和中东以及南美均有流行,在非流行区也有散发病例。该病毒具有多型性、易变的特点。     

猪口蹄疫的特点与防治

1 口蹄疫病毒特征 口蹄疫是由口蹄疫病毒(FMDV)感染引起的偶蹄动物共患的急性、热性、高度接触性传染病,最易感染的动物是牛、猪、骆驼、羊、鹿等,野猪、野牛等野生动物也易感染此病.本病以牛最易感,羊的感染率较低.口蹄疫在亚洲、非洲和中东以及南美均有流行,在非流行区也有散发病例.该病毒具有多型性、易变的特点.口蹄疫病毒有O、A、C、Asial、SAT1、SAT2、SAT3等7个血清型和60多个亚型.     

家畜口蹄疫的流行特点

口蹄疫是由口蹄疫病毒所引起的偶蹄动物共患的急性、热性、接触性传染病,主要侵害牛、猪、绵羊、山羊和骆驼等家畜及野生偶蹄动物。本病有强烈的传染性,一旦发生会造成广泛流行。1动物易感性,口蹄疫病毒侵害多种动物,以偶蹄兽为最易感染。     

猪线粒体抗病毒信号蛋白MAVS对口蹄疫病毒感染宿主的影响

采用构建猪源MAVS重组质粒pEGFP-MAVS,将其转染PK-15细胞24h后,再感染口蹄疫病毒(MOI=0.1),通过Western blot和real-time PCR检测MAVS融合蛋白表达及对口蹄疫病毒感染宿主细胞的复制和病毒感染滴度。结果显示:重组质粒pEGFP-MAVS能在PK-15细胞中获得表达,并且上调MAVS基因对口蹄疫病毒在宿主细胞中的早期复制有显著抑制作用,病毒的感染滴度也有一定的降低。结果表明:MAVS具有一定的抗病毒作用,此为进一步研究口蹄疫病毒逃逸宿主天然免疫的机制奠定了基础。     

人参多糖体外抗猪生殖与呼吸综合征病毒活性

猪繁殖与呼吸综合征(porcine reproductive and respiratory syndrome,PRRS)是严重危害养猪业的传染病之一,也是重点防控的疾病之一。人参多糖(ginseng polysaccharides complex,GPS)具有抗病毒及增强免疫的作用。为了寻找有效预防和治疗PPRS的药物,本试验以Marc-145细胞为模型,采用细胞病变抑制试验和MTT法测定细胞活力,观察GPS在体外抗猪繁殖与呼吸综合征病毒(porcine reproductive and respiratory syndrome virus,PRRSV)的作用,并通过改变药物作用方式初步探讨GPS的抗病毒机制。试验分为6组,分别为50,100,200和400 mg/L GPS组、病毒对照组及正常细胞对照组。在GPS对PRRSV感染Marc-145细胞的阻断和抑制作用中,各质量浓度GPS均显著提高感染PRRSV后Marc-145细胞的存活率(P<0.05),GPS处理组Marc-145细胞中的PRRSV量均显著低于病毒对照组(P<0.05)。而且,GPS可以促进Marc-145细胞中IL-6和IFN-γ的mRNA表达,也能增强细胞上清中IL-6和IFN-γ的活性。本试验研究表明GPS在体外可有效阻断或抑制PRRSV对Marc-145细胞的感染,提示GPS可作为预防和治疗PRRS的候选药物。     

口蹄疫疫苗效检模型动物测毒及口蹄疫种毒冻干试验

应用豚鼠作为口蹄疫疫苗效检模型动物检测口蹄疫病毒对模型动物的病原性.用体重400 g左右的豚鼠,将猪O型口蹄疫灭活疫苗效检攻毒毒株ORMF8经后肢蹠部皮内注射途径进行测毒.测毒结果表明,口蹄疫病毒可引起豚鼠出现典型发病,并产生明显病变,ORMF8种毒对豚鼠的毒价可达105.5ID50/0.2 mL.将乳鼠中和试验用种毒OMⅡ按一定比例加入5%蔗糖脱脂牛奶稳定剂进行冷冻真空干燥试验,3次冻干试验结果表明,种毒冻干后病毒含量有一定程度的下降,但下降程度不显著.     

猪α干扰素的原核表达及抗高致病性猪繁殖与呼吸综合征病毒活性测定

为了研究高效广谱的基因工程抗病毒制剂,本研究采用PCR技术自猪肝脏总DNA中扩增、克隆猪α干扰素（PoIFN-α）成熟肽基因并亚克隆入pQE30载体进行原核表达,对表达的融合重组猪α干扰素（rPoIFN-α）蛋白通过尿素变性、低浓度蛋白复性液复性、PBS溶液透析等步骤进行纯化,采用细胞病变抑制法分别测定rPoIFN-α蛋白在PK15细胞上抗水泡性口炎病毒（VSV）增殖活性及在Marc-145细胞上抑制高致病性猪繁殖与呼吸综合征病毒（PRRSV）的增殖活性,以分别评价rPoIFN-α的活性单位及其体外抑制高致病性PRRSV增殖所需的最低浓度。结果表明,克隆的PoIFN-α成熟肽基因全长501bp,编码166个氨基酸;表达的rPoIFN-α蛋白分子量大小20.7ku左右,与预期大小相一致;经纯化后的蛋白纯度在95%以上;纯化的rPoIFN-α蛋白在PK15细胞上抗VSV病毒活性单位为1.4482&#215;10^4IU/mL（5.2&#215;10^6IU/mg）,在Marc-145细胞上能完全抑制高致病性PRRSV增殖所需的rPoIFN-α蛋白最低有效剂量为640U/mL（2.3&#215;10^4U/mg）。这些研究结果为新型基因工程抗病毒制剂的开发以及用于高致病性PRRSV性疾病的预防和治疗奠定了基础。     

The efficacy of FMD vaccine reduced non-structural proteins with a mAb against 3B protein

A monoclonal antibody, 3BIgG, against the prokaryotically expressed foot-and-mouth disease virus (FMDV) non-structural protein (NSP) 3B was obtained. The 3BIgG-sepharose conjugant (3BmAb-6BFF) was prepared by adding the purified 3BIgG into epoxy-activated sepharose 6BFF, incubating with the inactivated FMDV, and then removing the sepharose by centrifugation. The vaccine was made from the supernatant emulsified with oil-adjuvant ISA206. Ten guinea pigs, 26 pigs and six cattle were vaccinated, and a vaccination control group was included without treatment with 3BmAb-6BFF. After 28 days, 9/10 pigs challenged with FMDV were protected, this result was the same as the control group, indicating that the vaccine potency was not reduced after treatment with 3BmAb-6BFF. The other animals were vaccinated weekly for nine weeks, and serum samples were collected to detect 3ABC-antibody titers. The results showed that 3ABC-antibody production was delayed and the positive antibody rates were lower when vaccination was carried out using vaccines treated with 3BmAb-6BFF compared with untreated vaccines. The findings of this study suggest that it is possible to reduce NSPs using a mAb-sepharose conjugant in FMD vaccines without reducing their efficacy.     

Development and characterization of monoclonal antibodies against FMD virus type Asia-1 and determination of antigenic variations in the field strains

Twelve mouse monoclonal antibodies (MAbs) were developed against an Indian vaccine strain of foot and mouth disease virus (FMDV) type Asia-1 WBN 117/85. The MAbs were tested for their ability to bind to whole virus particle, trypsin-treated 146S (TT-146S) virus particle, sub-viral (12S and disrupted virus) antigens by ELISA and to neutralize virus infectivity in cell culture. Extensive characterization of MAbs revealed the existence of three different groups based on the binding of non-overlapping epitopes. Eight type Asia-1 specific MAbs (RF7, RF8, RD10, RE11, RC11, RC10/O, RB11 and RC10/M), which formed group 1 (G1), were found to bind a neutralizing, trypsin-sensitive (TS) and conformational epitope. Two MAbs (WB8 and WC3) in group 2 (G2) were found to bind a non-neutralizing, trypsin-resistant, conformational and 12S-specific epitope, which was intertypically conserved in all the four serotypes of FMDV (O, A, C and Asia-1) prevalent in India. Two MAbs (KG10 and KF10), which formed group 3 (G3), were found to be against a non-neutralizing, TS and conformational epitope, common to types Asia-1 and A. Members of G1 were IgG2a isotype, while those of G2 and G3 were IgG1 and IgG2b isotypes, respectively. Antigenic analysis of 31 FMDV type Asia-1 field isolates and two vaccine strains, using a panel of type Asia-1-specific MAbs, revealed antigenic similarity of the virus isolates tested and non-existence of neutralization escape mutants. The developed MAbs have practical utility, especially in the manufacture of FMD vaccine, diagnosis and FMDV characterization.