Assessment of the burrow system of Lumbricus terrestris, Aporrectodea giardi, and Aporrectodea caliginosa using X-ray computed tomography

Artificially packed soil columns were inoculated with individuals of different earthworm species (Lumbricus terrestris, Aporrectodea giardi or Aporrectodea caliginosa) and placed under controlled conditions in the laboratory. At the end of the incubation period, which lasted 8 months, three-dimensional reconstructions of the burrow system of each species were obtained using X-ray computed tomography. The particular features of the three burrow systems and the differences between them are discussed in terms of density, orientation and distribution of the burrows and the complexity of the network in relation to variations in soil depth and soil density. Received: 5 February 1997     

Quantitative analysis of earthworm burrow systems with respect to biological soil-structure regeneration after soil compaction

 On arable land, tilled with conventional tillage (CT) and conservation tillage (CS) respectively, plots were compacted by wheeling them 6 times with a 5 Mg wheel load in spring 1995. Immediately after compaction, undisturbed soil monoliths were excavated from the compacted and uncompacted plots. The monoliths were defaunated and inoculated with either Lumbricus terrestris or Aporrectodea caliginosa. One monolith from each plot remained uninoculated as a control. After 6 months the monoliths were defaunated again and then scanned with X-ray helical computed tomography. The data were transformed, the void systems inside the monoliths were reconstructed and visualised, and the parameters total void length, total void volume, tortuosity and continuity were quantified. The parameters' values were generally lower in the controls than in the inoculated monoliths. Differences in burrow construction could be explained by the different life strategies of the two earthworm species. Changes in burrow morphology due to tillage system and soil compaction were minor. Only the continuity of the burrow systems clearly changed: decreasing for L. terrestris and increasing for A. caliginosa. This can be explained by a change in the earthworms' burrowing activity to minimise energy expenditure in compacted soil. By extrapolating field data, we concluded that earthworms have great potential for biologically regenerating the soil structure after a single compaction event. Due to higher earthworm abundances in soil managed by CS the regeneration of the soil structure is assumed to be better in these plots than those tilled by CT. Received: 17 December 1997     

Three-dimensional visualization and quantification of soil macroporosity and water flow patterns using computed tomography

Abstract. Efforts to assess the role of soil structure in soil and water processes have been limited by data based on two dimensional geometry or disturbed samples. Computed tomography (CT) is a non-invasive technique that allows for the three dimensional, non-destructive examination of heterogeneous materials. X-ray CT scanning was used to acquire serial images of contrasting soil types that could be used to render, and hence, quantify important soil constituents in three dimensions. By repeating the scanning techniques after an infiltration period, it was also possible to examine the nature of water movement in real time. Macropore architecture between different soil types was highly variable and generally appeared as unconnected pore segments at the resolution examined. As expected, water flow characteristics were similar to macropore structure, with wetting patterns resembling both Darcian and preferential flow identified in different soils. From this, it was found that in the sandy clay soil c . 90% of macropore space was active following infiltration, compared with c . 50% for the sandy loam soil. In addition, it was possible to visualize the three dimensional distribution of stones and other mineral material. The results illustrated the benefits of computed tomography over more conventional analyses of soil structure, and its potential as a tool for examining dynamic soil processes. However, problems associated with accessibility, resolution and artefacts still provide limitations of the technique.     

A non-destructive method for the morphological assessment of earthworm burrow systems in three dimensions by X-ray computed tomography

Summary Earthworm burrows of endogeic species (Allolobophora caliginosa, Octolasium cyaneum) in artificially packed soil columns were examined using X-ray computed tomography. By means of digital image processing, it was possible to reconstruct and visualize the burrow system in three dimensions. The reconstruction revealed morphological features of the burrows which were not obvious from two-dimensional section images.     

3D skeleton reconstructions of natural earthworm burrow systems using CAT scan images of soil cores

 Four soil cores (length, 20 cm; diameter, 16 cm) were sampled in a Swiss pre-Alpine meadow with high earthworm abundance (>400 individuals/m²); two cores were taken in October 1993 and the other two cores in April 1994. The cores were described using computer assisted tomography which gives a series of section images every 3 mm. A method for reconstructing the three-dimensional (3D) skeleton of the earthworm burrow system is presented and discussed. This method provides an image of the structural organisation of the burrow system and was found to be adequately sensitive for use in ecological and functional studies. The seasonal variation of these 3D skeletons was investigated using two approaches, i.e. the analysis of: (1) global burrow system characteristics, and (2) individual burrow characteristics. At the scale of the global burrow system no difference was found between seasons (same number of burrows and same total burrow length) except for the vertical segment distribution, which was homogeneous in spring and decreased with depth in the fall. The study of individual burrow characteristics revealed that burrows tended to be more vertical in spring and that their branching intensity was higher in this season. Received: 10 June 1997     

Evolution of burrow systems after the accidental introduction of a new earthworm species into a Swiss pre-alpine meadow

 The unintentional introduction of a new earthworm species (Aporrectodea nocturna) into a Swiss pre-alpine meadow resulted in a great increase in earthworm density in the newly colonized area (386 m^–2) compared with the density observed in the natural area (273 m^–2) where an earthworm community was already present. To investigate the impact of this introduction on the burrow systems, eight soil cores (length 25 cm, diameter 16 cm) were taken (four in the colonized area and four in the natural area) and analysed with computer-assisted X-ray tomography. The resulting images were processed to obtain the 3D-skeleton reconstructions of the earthworm burrow systems. Due to high variability in these burrow systems, only slight differences were observed between the two areas. The total burrow length and the mean burrow lengths tended to be greater in the colonized area. Moreover, the distribution of pore numbers with depth showed different patterns with a maximum for depths between 10 cm and 15 cm in the colonized area and a maximum for depths between 20 cm and 25 cm in the natural area. These differences may have been related to: (1) the particular behaviour of A. nocturna, which was observed to cast at the surface in this site, and (2) the predominance of juvenile earthworms around the colonization front. These differences were sufficient to create significant effects on the continuity of the burrow systems (assessed by the number of different pathways between virtual horizontal planes) for the two areas. The colonized area was characterized by a greater pore continuity, which could have resulted in enhanced transfer properties. Received: 2 July 1999     

Characterisation of the three-dimensional structure of earthworm burrow systems using image analysis and mathematical morphology

The aim of this work was to exemplify the specific contribution of both two- and three-dimensional (3D) X-ray computed tomography to characterise earthworm burrow systems. To achieve this purpose we used 3D mathematical morphology operators to characterise burrow systems resulting from the activity of an anecic ( Aporrectodea nocturna), and an endogeic species ( Allolobophora chlorotica), when both species were introduced either separately or together into artificial soil cores. Images of these soil cores were obtained using a medical X-ray tomography scanner. Three-dimensional reconstructions of burrow systems were obtained using a specifically developed segmentation algorithm. To study the differences between burrow systems, a set of classical tools of mathematical morphology (granulometries) were used. So-called granulometries based on different structuring elements clearly separated the different burrow systems. They enabled us to show that burrows made by the anecic species were fatter, longer, more vertical, more continuous but less sinuous than burrows of the endogeic species. The granulometry transform of the soil matrix showed that burrows made by A. nocturna were more evenly distributed than those of A. chlorotica. Although a good discrimination was possible when only one species was introduced into the soil cores, it was not possible to separate burrows of the two species from each other in cases where species were introduced into the same soil core. This limitation, partly due to the insufficient spatial resolution of the medical scanner, precluded the use of the morphological operators to study putative interactions between the two species     

Quantification of the effects of soil compaction on water flow using dye tracers and image analysis

Abstract. Soil compaction has long been considered to be a problem in arable land, primarily because it causes damage to soil structure, which can lead to serious reduction in crop yields. However, few studies have sought to investigate the effects of soil compaction on the water transport regime of modified soil pore systems. We attempted to quantify the effects of soil compaction on the initiation of preferential flow by using dye tracers and image analysis. A laboratory methodology involving rainfall simulation enabled us to quantitatively evaluate differences in the mechanisms of water flow between two soil types at several degrees of compaction. The results suggested significant differences in the types of water flow pathways between clay loams and sandy loams at different extents of compaction. In the sandy loam, it was concluded that a high degree of compaction led to an increased likelihood of preferential flow, whereas a more uniform movement of soil water occurred at less compaction. By contrast, preferential routing of soil water was recorded in the clay loam, except at the highest measured compaction. The results indicate that the visual techniques of dye tracing and image analysis could enable improved understanding of flow pathways of soil water associated with soil compaction.