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1.
Defence proteins are a kind of chemical defence compounds. They play a key role in plant restraining biotic and abiotic harm. To illuminate activities of some defense proteins associated with age or plant family of larch, the larch needles were collected from two different families of Korean larch, Larix olgensis and a hybrid larch, L. kaempferi × L. gmelinii, respectively, and then the activities of defence proteins in those were tested using a UV spectrophotometry. The results showed that the activities of protective enzymes at the16-/17- and 19-year-age groups were higher than those at the other age groups in the both larch species. While the activities of polyphenol oxidase (PPO) at the 16-/17-year-age group and phenylalanine ammonia-lyase (PAL) and protease inhibitors at the 19-year-age group were the highest among all age groups. Then, compared with L. olgensis, the hybrid larch had significant effects on the activities of protective enzymes. The effects of plant family on the activities of PAL and chymotrypsin inhibitor were significantly different, and then those on the activities of PPO were not significantly different. The effects of the two families in L. olgensis on the activities of trypsin inhibitor (TI) were significantly different, while those in the hybrid larch on the activities of TI were contrary. To conclusions showed that the different age and plant family significantly affected the activities of defence proteins in the needles of two larch species, and then enhanced the larch resistance to pests. These could play a key function in forestry tree genetic improvement and management in future. 相似文献
2.
Xudong He Fagen Li Mei Li Qijie Weng Jisen Shi Xiaoyong Mo Siming Gan 《New Forests》2012,43(3):383-394
Interspecific hybrids of Eucalyptus urophylla S. T. Blake × E. tereticornis Smith were used for quantitative genetic analysis of cold hardiness (CH) and growth traits height (H), diameter at breast height (D) and volume (V) at 0.5, 1.5, 2.5 and 4 years of age. The effect of E. tereticornis male variance was significant (P < 0.05) for CH as well as 2.5- and 4-year-old growth except for H
4, and E. urophylla female × E. tereticornis male interaction was significant for 0.5- and 1.5-year-old growth and CH except for CH
0.5, whereas E. urophylla female effect was not significant for the majority of traits analyzed. Both additive and dominance gene action were present
in the genetics of all the traits measured at most ages, indicating that those traits may be improved by hybrid breeding.
The σ
D
2/σ
A
2 ratios tended to decline after 1.5 years of age in CH and growth traits, implying that breeding value predictions derived from older performance might be more reliable. Estimates
of narrow-sense heritability (h
2
) for CH and growth ranged from 0.03 ± 0.05 to 0.28 ± 0.13, depending on trait and age. Additive genetic correlations between CH and V were 0.28 ± 0.48 and 0.69 ± 0.30 at ages 1.5 and 2.5, respectively, suggesting that selection for growth would cause a favorable
increase in cold hardiness in E. urophylla × E. tereticornis hybrids. 相似文献
3.
Soil microbes may be critical players in determining the allelopathic potential of some plants. Low levels of plant community biodiversity in Eucalyptus plantations have been attributed to the allelopathic potential of these tree species. The role of soil microbes in the allelopathic effect of leaf leachates of the hybrid tree Eucalyptus grandis × E. urophylla, was tested in Petri dish assays with Brassica chinensis as a receiver plant. Soils were collected from either a local garden (soil A) or a Eucalyptus plantation (soil B) and half of each soil was sterilized to remove microbes. These soils were then treated with E. grandis × E. urophylla leachates for 0–72 h. Seed germination of B. chinensis was significantly inhibited in soils treated with leaf leachates relative to untreated soils. The inhibitory effect of the leaf leachates was more pronounced in sterilized soils. Total phenolic content was obvious lower in nonsterile leachate-treated soils than in sterile soils. Biomass of B. chinensis was negatively correlated with the total phenolic content in soils. Our findings suggest that soil microbes can alleviate the allelopathic potential of Eucalyptus and thereby its negative impact on plant growth. 相似文献
4.
Jianming Gao Yong Zhang Chunguo Wang Shougong Zhang Liwang Qi Wenqin Song 《New Forests》2009,37(3):333-344
Amplified fragment length polymorphism (AFLP) was used in genetic fingerprinting of 22 elite Chinese Populus deltoides and Populus × canadensis accessions. The results indicated that each of the nine AFLP primer pairs selected generated fingerprint profiles that were
unique to each of the accessions. Therefore, each accession was definitively identified by any of the nine primer pairs. Furthermore,
the inter-accession genetic relationships inferred based on 461 polymorphic fragments from the nine AFLP primer pairs were
largely consistent with phylogenetic relationships based on morphologic traits. Bootstrap analysis showed that three AFLP
primer pairs were required to obtain genetic similarity values with a maximum CV of 10% while 10 AFLP primer pairs could give
a maximum CV of 5%. Thus, AFLP can readily be applied for a rapid and accurate evaluation of the degree of similarity between
poplar cultivars. In this study, the number of AFLP fragments used was sufficient to establish a reliable estimate of genetic
similarity among accessions, with a maximum CV of 5.12%. Therefore, the information on the genetic relationships among the
poplar accessions generated in this study in connection with knowledge on agronomic traits may have an impact on poplar breeding
and planting in China. 相似文献
5.
Elias Milios Kyriaki Kitikidou Panagiota Andreadou 《European Journal of Forest Research》2017,136(5-6):971-979
The aims of this study were to analyze the sprouting characteristics of Quercus pubescens and Q. frainetto trees after a wildfire and to recommend the appropriate silvicultural treatments in order to achieve dense sprout origin regeneration. This study was carried out in the area that was burned in a wildfire in northeastern Greece. Three years later, 140 Q. pubescens trees and 140 Q. frainetto trees were selected. Seventy trees for each species were individuals that were cut after the fire and were in the form of stumps, while 70 trees were intact top-killed trees, as a result of fire. In each stump and intact tree that was selected, various parameters were measured or counted: the diameter at the level of ground, the number of the developed sprouts (if any) and the height as well as the basal diameter of the tallest sprout, etc. Both species have similar sprouting behavior after a wildfire. Mother tree dimensions positively influenced the number of sprouts and the growth of the tallest sprout per individual. The mean height of the tallest sprout per stump, after three growing seasons from the forest fire, is high in both species (2.08 m for Q. pupescens and 2.29 m for Q. frainetto). Cut stumps had more sprouts than intact trees in both species. Forest practice, in order to maximize the sprouting of Q. pubescens and Q. frainetto trees after a forest fire, should cut the burned trees before the next growing season. 相似文献
6.
Cassiana de Oliveira Juliana Degenhardt-Goldbach Gisela Manuela de França Bettencourt Erika Amano Luziane Franciscon Marguerite Quoirin 《林业研究》2017,28(1):29-39
Genetic transformation systems require protocols that allow regenerating transgenic plants from transformed tissues. This study aimed to establish a protocol for indirect organogenesis in leaf explants of a Eucalyptus grandis × E. urophylla AEC 224 clone. During callogenesis stage, several concentrations of NAA and then NAA or 2,4-D combined with TDZ were tested in JADS culture medium for 30 days, followed by subculture of the explants in the regeneration medium, containing 5.0 µM BA and 0.5 µM NAA for another 30 days. In these media, the explant oxidation rate was high (95 %). Thus, in order to reduce oxidation, different culture media were compared: WPM, MS, JADS and modified QL, followed by explant transfer onto regeneration medium. The highest percentage of regeneration and the lowest oxidation rate were achieved on WPM medium. Then, NAA and 2,4-D were tested in combination with TDZ and also TDZ and BA combined with NAA in WPM medium. The most efficient culture media in terms of shoot regeneration were WPM supplemented with 0.25 µM TDZ and 0.1 µM NAA during 30 days for callus induction and then with 5.0 µM BA and 0.5 µM NAA for another 30 days. This protocol yielded a regeneration rate of 43 %, with a low oxidation of tissues. A rooting experiment was conducted using half strength MS medium and comparing three concentrations of IBA (2.46, 4.90 and 7.35 µM). The highest rooting percentage (35 %) was obtained on medium containing 2.46 µM IBA. Once the shoots were rooted, acclimatization in a greenhouse was not challenging and plant survival reached 100 %. 相似文献
7.
Five tannin-related compounds – gallic acid, flavogallonic acid dilactone, valoneic acid dilactone, gallagyldilactone, ellagic acid – were isolated from the heartwood of Shorea laeviforia, and the inhibitory activity of each against rat liver 5-reductase was evaluated. Valoneic acid dilactone and gallagyldilactone exhibited positive inhibitory activity, but gallic acid and ellagic acid did not. Flavogallonic acid dilactone stimulated 5-reductase activity, even though this compound is structurally similar to valoneic acid dilactone. The kinetic study of valoneic acid dilactone and gallagyldilactone indicated that the inhibitory behavior of 5-reductase were not competitive against the substrate (testosterone) and were partially competitive against the cofactor (NADPH). Additionally, double inhibition analysis of valoneic acid dilactone and NADP+ showed synergetic inhibition. These results suggested that neither valoneic acid dilactone nor gallagyldilactone can affect the binding of testosterone but that either compound could interact with an enzyme–NADP+ complex to inhibit 5-reductase. 相似文献
8.
Yoshie Terashima 《Journal of Wood Science》2013,59(1):80-87
An unidentified Fomitiporia sp. causes severe white-rot on stems of a cultivar “Sanbu-sugi” of the Japanese cedar, Cryptomeria japonica. The influence of the fungus on tree health and wood properties was determined. Bark from dead branches and xylem from living branches contained more glucose than bark from living branches and xylem from dead branches. Tree heights at which annual rings were disconnected were 2, 4 and 6 m at ages 37, 15 and 24 years old, respectively. The pH values of damaged parts were lower than those of non-damaged parts, and the damaged parts were clearly identified using bromocresol green solution. Weight loss of sapwood during 60 days of fungal degradation was 1.4 %, which was less than that by a saprophyte, Trametes versicolor. The amount of polyphenols in the heartwood from damaged tree stems was less than that from non-damaged stems. Degraded parts were less stiff than the non-degraded sapwood as measured with a wood-decay tester, Pilodyn. Our observations indicate that damaged stems are chemically and physically inferior to non-damaged stems. 相似文献
9.
The effect of Thidiazuron (TDZ), basal media and light quality on adventitious shoot regeneration from in vitro cultured stem of Populus albaxP berolinensis were determined to establish a high efficiency shoot regeneration system from stem explants of P. alba~P berolinensis. Stems ofPopulus alba~P berolinensis were collected from cultured shoots in vitro derived from dormancy buds of 3-year-old seedlings. The stem explants were cultured on MS medium containing 0.02-mg·L-1NAA (naphthaleneacetic acid), and 0.1, 0.3, 0.5 and 1.0 mg·L-1 concentrations of TDZ to determine the effect of TDZ on shoot regeneration. Three basal media, i.e. MS, woody plant medium (WPM) and B5, were used to test their influences of different media on adventitious shoot regeneration. Green, red, blue and yellow plastic films in comparison with florescent light as control were used to observe their effects on shoot regeneration. The results showed that differ- ent concentrations of TDZ had an evident influence on shoot regeneration. Lower concentration of TDZ (0.1 mg·L-1) resulted in more ad- ventitious shoot regeneration and higher concentration of TDZ (〉0.1 mg·L-1) inhibited shoot regeneration. Among different media, MS medium exhibited a high efficiency for shoot regeneration, followed by WPM medium, while B5 medium inhibited shoot regeneration. Normal light and yellow light exhibited better effects on shoot regeneration, compared with other light. 相似文献
10.
Gerald Dirnberger Angela-Elisabeth Kumer Eduard Schnur Hubert Sterba 《Annals of Forest Science》2017,74(1):8
Key message
Trees with otherwise equal dimensions have different leaf areas if they are located in different stand types. While leaf area of European larch is affected by mixture proportion, leaf area of Norway spruce is affected by stand density.Context
Leaf area is a key parameter for evaluating growth efficiency of trees, and therefore needs to be measured as consistently and accurately as possible. This is even more important when comparing monospecific and mixed stands.Aims
The aim of the study is to find combinations of parameters and allometric relationships that can be used to estimate accurately the leaf area of individual trees.Methods
Allometries of the measured leaf area of 194 trees in 12 stands were analysed in order to find variables affecting leaf area. Existing functions from the literature were validated. Finally, models were fitted to find the most appropriate method for estimating leaf area of mixed and monospecific stands of Norway spruce and European larch.Results
Allometric relationships of leaf area to other measurable characteristics of trees vary in different stand types. Besides individual tree dimensions such as diameter and crown surface area, leaf area of Norway spruce is related to stand density, whereas the leaf area of European larch is dependent on the admixture of Norway spruce in the stand.Conclusion
In contrast to models for estimating individual tree leaf area of Norway spruce, models for leaf area of European larch have to consider mixture proportions in order to correctly interpret the growth efficiency of mixed stands.11.
Jiaojun Zhu Kai Yang Qiaoling Yan Zugen Liu Lizhong Yu Hexin Wang 《Journal of Forest Research》2010,15(1):71-80
Thinning experiments were conducted in larch (Larix olgensis) plantations to assess the feasibility of converting even-aged plantation stands to uneven-aged forests with more complex
stand structures. Stands established in 1965 and 1960 were thinned in 2004 (Regime A, for determining the effect of recent
thinning on emergence of seedlings) and 1994 (Regime B, for examining the effects of the past thinning on establishments of
recruitments), respectively, at two intensities each. Natural regeneration, together with litter depth, canopy openness and
vegetation cover, was surveyed in the thinned plots. Results indicated that larch seedlings started to emerge in May, reached
a peak in June, decreased from June through September, and then disappeared in October. No larch seedlings exceeded 1 year
old in the thinned plots because of the low levels of light and dense litter and vegetation cover. However, there were many
naturally regenerated seedlings (5–50 cm in height) and saplings (50–500 cm in height) of broadleaved tree species such as
Acer spp., Fraxinus spp., Cornus controversa, Quercus mongolica, and even the climax tree species, Pinus koraiensis, in the thinned plots. The mean density of regenerated seedlings reached 6.7 and 4.5 stems m−2 in Regimes A and B, respectively, whilst the mean density of regenerated saplings reached 4,595 stems ha−1 in Regime B. These results suggest that it is impractical to turn even-aged larch plantations to uneven-aged larch forests,
but it may be feasible to develop uneven-aged larch-broadleaved forests from even-aged larch plantations through thinning. 相似文献
12.
The effect of addition of Acacia mangium bark powder on the thermosetting processes of two commercial phenol resins, PF-A and PF-B, was examined by bond strength
test, torsional braid analysis, and differential scanning calorimetry. When the bark powder was added to PF-A, the bond strength
of plywood pressed at 110°C increased and was comparable to that of plywood pressed at 120 and 130°C. However, when the bark
powder was added to PF-B, the bond strength of plywood pressed at 110°C was still lower than that of plywood pressed at 120
and 130°C. The relative rigidity and loss tangent of PF-A cured with the bark powder obtained by heating at 100°C were comparable
to those at 120 and 140°C, and the reaction enthalpy was increased by bark powder addition. In contrast, chemical reactions
for cured PF-B were not enhanced by bark powder addition. 相似文献
13.
Microsporogenesis and flower development in Eucalyptus urophylla × E. grandis were examined using chromosome tableting to provide a method to predict the meiotic stages in this species. Although microsporogenesis was normal, cytokinesis during meiosis of pollen mother cells occurred simultaneously, with strong asynchronism observed in the two different lengths of stamens in a flower bud. In a single flower, the developmental period of microsporogenesis in anthers on the longer stamens was always ahead of those on the shorter stamens. Flower development was also asynchronous at different locations on a branch. Flower buds on the upper side of the branch were larger in diameter than those on the lower side. In addition, a correlation was observed between microsporogenesis development and flower bud diameter growth. The pachytene stage was first observed when the diameter of the flower buds increased to 3.0 mm, and the majority of the meiotic stages were observed when bud diameters ranged from 3.5 to 5.0 mm. This study showed that the developmental stages of microsporogenesis in Eucalyptus urophylla × E. grandis could be distinguished readily, which may be applicable to future breeding studies. 相似文献
14.
Leaf functional traits are adaptations that enable plants to live under different environmental conditions. This study aims to evaluate the differences in leaf functional traits between red and green leaves of two evergreen shrubs Photinia × fraseri and Osmanthus fragrans. Specific areas of red leaves are higher than that of green leaves in both species. Thus, the material investment per unit area and per lamina of red leaves is significantly lower than that of green leaves, implying an utmost effort of red leaves to increase light capture and use efficiency because of their low leaf-chlorophyll concentration. The higher petiole length of green leaves compared with that of red leaves indicates that adult green leaves may have large fractional biomass allocation to support the lamina structures in capturing light with maximum efficiency and obtaining a high growth rate. The high range of the phenotypic plasticity of leaf size, leaf thickness, single-leaf wet and dry weights, and leaf moisture of green leaves may be beneficial in achieving efficient control of water loss and nutrient deprivation. The high range of phenotypic plasticity of leaf chlorophyll concentration of red leaves may be advantageous in increasing resource (especially light) capture and use efficiency because this leaf type is juvenile in the growth stage and has low leaf-chlorophyll concentration. 相似文献
15.
The optimum conditions for β-thujaplicin production in a Cupressus lusitanica cell suspension culture were investigated. The conditions required for β-thujaplicin production were clearly different from the conditions for cell growth. The initial phosphate concentration and pH did not affect β-thujaplicin production. A total nitrogen source concentration higher than 3.2 mM suppressed production due to the presence of the ammonium ion. β-Thujaplicin production was observed at 95 mg/l without adding the ammonium ion to the medium. Strict control of major inorganic nutrients was not necessary to produce β-thujaplicin. This finding seems to be favorable for future automated production of β-thujaplicin in commercial cell culture plants. 相似文献
16.
John Mukuka Olaf Strauch Christopher Hoppe Ralf-Udo Ehlers 《Journal of pest science》2010,83(3):281-287
Entomopathogenic nematodes of the genus Heterorhabditis are a suitable alternative to chemical insecticides because of their high control effect against insects in cryptic environments
and their environmental safety. The availability of H. bacteriophora hybrid strains with increased tolerance to environmental extremes could be a driving force for a more widespread use of nematode-based
plant protection products. However, increase in heat and desiccation tolerance of hybrid strains could compromise their fitness
regarding virulence, host penetration and reproductive capacity. The fitness of heat and desiccation tolerant hybrid strains
was compared to the commercial strain EN 01. Only the heat tolerant strains were superior or similar in fitness to strain
EN 01. The strains with increased desiccation tolerance were usually less fit, a possible result of a trade-off effect of
selection for desiccation tolerance. Hybrid strains selected for enhanced tolerance after an adaptation to stress were generally
better in fitness ranking compared to those for which adaptation prior to stress exposure was excluded. This might be a result
of pleitropy. Host penetration and virulence was not correlated. The commercial strain had the highest reproduction per mean
number of nematodes penetrating the host insect, which is a result of automatic selection of inbred lines with high reproduction
potential during the commercial production process in liquid culture. 相似文献
17.
The role of plant eIF5A proteins in multiple biological processes, such as protein synthesis regulation, translation elongation, mRNA turnover, programmed cell death and stress tolerance is well known. Toward using these powerful proteins to increase stress tolerance in agricultural plants, in the present study, we cloned and characterized PsneIF5A2 and PsneIF5A4 from young poplar (P. simonii × P. nigra) leaves. The deduced amino acid sequences of PsneIF5A2 and PsneIF5A4 were 98 % similar to each other, and they are orthologs of eIF5A1 in Arabidopsis. In a subcellular localization analysis, PsneIF5A2 and PsneIF5A4 proteins were localized in the nucleus and cytoplasm. qRT-PCR analysis showed that PsneIF5A2 and PsneIF5A4 were transcribed in poplar flowers, stem, leaves, and roots. In addition, they were also induced by abiotic stresses. Transgenic yeast expressing PsneIF5A2 and PsneIF5A4 had increased salt, heavy metal, osmotic, oxidative tolerance. Our results suggest that PsneIF5A2 and PsneIF5A4 are excellent candidates for genetic engineering to improve salt and heavy metal tolerance in agricultural plants. 相似文献
18.
Shengkan Chen Qijie Weng Fagen Li Mei Li Changpin Zhou Siming Gan 《Annals of Forest Science》2018,75(1):16
Key message
Growth and wood chemical properties are important pulpwood traits. Their narrow-sense heritability ranged from 0.03 to 0.49 in Eucalyptus urophylla × E. tereticornis hybrids, indicating low to moderate levels of genetic control. Genetic correlations were mostly favorable for simultaneous improvement on growth and wood traits. Additive and non-additive genetic effects should be considered in making a hybrid breeding strategy.Context
Eucalypt hybrids are widely planted for pulpwood production purposes. Genetic variations and correlations for growth and wood chemical traits remain to be explored in Eucalyptus interspecific hybrids.Aims
Our objectives were to clarify the heritability of growth and wood chemical traits and determine the genetic correlations between traits and between trials in E. urophylla × E. tereticornis hybrids.Methods
Two trials of 59 E. urophylla × E. tereticornis hybrids derived from an incomplete factorial mating design were investigated at age 10 for growth (height and diameter) and wood chemical properties (basic density, cellulose content, hemi-cellulose content, lignin content, and syringyl-to-guaiacyl ratio). Mixed linear models were used to estimate genetic parameters.Results
Narrow-sense heritability estimates were 0.13?0.22 in growth and 0.03?0.49 in wood traits, indicating low to moderate levels of additive genetic control. Genetic correlations were mostly positively significant for growth with basic density and cellulose content but negatively significant with hemi-cellulose and lignin contents, being favourablefavorable for pulpwood breeding purpose. Type-B correlations between sites were significant for all the traits except diameter and lignin content.Conclusion
Hybrid superiority warrants the breeding efforts. An appropriate breeding strategy should be able to capture both additive and non-additive genetic effects.19.
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Introduction The simulation of diameter distributions is the basis for predicting volume in the so-called diameter distribution models.
Combined with volume, volume ratio and taper equations, these models allow the prediction of volume assortments according
to user needs. The simulation of diameter distributions is also essential in initialising individual tree models. It is also
a useful aid for planning harvesting operations. 相似文献
20.
We used energy dispersive X-ray fluorescence (EDXRF) to determine the seasonal variation of selected trace elements (Cr, Mn, Zn, Cu, Se and Fe) and some potential toxic elements (Cd, Pb and Br) in Juniperus thurifera subsp. africana Maire (Cupressaceae) a rare medicinal tree, growing indigenously in Aurès Mountains of Algeria. The precision of the results was assessed by analyzing the certified reference material IPE44 (WEPAL) grass leaves. Results showed J. thurifera was characterized by high Cr and Mn contents. During autumn and winter mineral concentrations were higher in general. The levels of Cr, Se and Mn were higher during autumn and winter than during spring and summer. Zn contents were higher during summer than in other seasons. Cu content did not vary by season. The potential toxic elements in J. thurifera (Pb > Cd > Br) were below the permissible limits recommended by the Joint WHO/FAO guidelines except for Pb in autumn and winter. 相似文献
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