共查询到20条相似文献,搜索用时 31 毫秒
1.
Jimenez M Guzman AP Azuara E Garcia O Mendoza MR Beristain CI 《Plant foods for human nutrition (Dordrecht, Netherlands)》2012,67(1):57-63
Porophyllum tagetoides is an annual warm-weather herb that has an intense typical smell. Its leaves are commonly used in soup preparation and traditional
medicine for treatment of inflammatory diseases. Its volatile compounds and antioxidant properties were evaluated in crude,
aqueous and ethanol leaf extract and an oil emulsion using different antioxidant assays in vitro, such as: DPPH radical scavenging activity, redox potential, polyphenol content, reducing power and optical density. A high
antioxidative activity was found when comparing leaves with stems. The crude extract from leaves showed a very high reducing
power (2.88 ± 0.20 O.D.) and DPPH radical-scavenging activity (54.63 ± 4.80%), in concordance with a major concentration of
vitamin C (23.97 ± 0.36 mg/100 g). Instead, the highest polyphenol content (264.54 ± 2.17 mg GAE/g of sample) and redox potential
(561.23 ± 0.15 mV) were found by the ethanol and aqueous extract, respectively. Aldehydes and terpenes such as nonanal, decanal,
trans-pineno, β-myrcene and D-limonene were the major volatiles found. This study suggests that Porophyllum tagetoides extracts could be used as antioxidants. 相似文献
2.
Ban X Huang B He J Chen Y Zeng H Han L Wang Y 《Plant foods for human nutrition (Dordrecht, Netherlands)》2011,66(2):175-180
The inflorescence of cultivated Coptis chinensis has been valued for tea production for many years in China. The antioxidant activities of C. chinensis inflorescence extracts prepared by various solvents were investigated by using several established in vitro systems: 2,2′-azinobis(3-ethylbenzthiazoline-6-sulphonic acid (ABTS), α,α-diphenyl-β-picrylhydrazyl (DPPH) and superoxide
radical scavenging assays, reducing power assay, and ferrothiocyanate (FTC) and thiobarbituric acid (TBA) assays. The results
showed that the 70% ethanol extract (EE) had the strongest antioxidant activity in vitro among the various extracts. Based on the in vitro results, EE was used to evaluate the antioxidant activity of C. chinensis inflorescence in vivo. The liver and kidney of intoxicated animals showed a significant decrease in superoxide dismutase (SOD), catalase (CAT)
and glutathione (GSH) levels, while the malondialdehyde (MDA) level showed a significant increase. These changes were significantly
reversed after treatment with EE and the standard vitamin E. Thus, the C. chinensis inflorescence may be a valuable natural source that can be applicable to food industries. 相似文献
3.
Fares R Bazzi S Baydoun SE Abdel-Massih RM 《Plant foods for human nutrition (Dordrecht, Netherlands)》2011,66(1):58-63
It is becoming increasingly evident that certain phytochemicals possess cancer chemopreventive properties. In this study,
the anti-proliferative activity of plant extracts from olive (Olea europaea L.) leaves was tested on human leukemic cell line (Jurkat). Cytotoxicity of various concentrations of plant extracts was examined
and the IC50 was determined. Olive leaf extracts showed concentration-dependent anti-proliferative effect as determined by the WST-1 proliferation
kit and [3H]-thymidine incorporation method. To study whether cell death was due to apoptosis, cells were stained with Annexin V-FITC
and PI and the expression of important regulatory proteins (Bcl-2, Bax, and p53) involved in apoptosis were examined by Western
blot. The antioxidant activity of olive leaves (SC50 = 0.1 mg dry weight) was studied using the DPPH scavenging method. Present findings suggest that olive leaves extracts exhibit
anti-proliferative effect on leukemic cells by inducing apoptosis. 相似文献
4.
Dias MI Barros L Sousa MJ Ferreira IC 《Plant foods for human nutrition (Dordrecht, Netherlands)》2011,66(2):181-186
Coriander is commonly used for medicinal purposes, food applications, cosmetics and perfumes. Herein, the production of antioxidants
in vegetative parts (leaves and stems) of in vivo and in vitro grown samples was compared. In vitro samples were clone A- with notorious purple pigmentation in stems and leaves and clone B- green. Seeds were also studied
as they are used to obtain in vivo and in vitro vegetative parts. Lipophilic (tocopherols, carotenoids and chlorophylls) and hydrophilic (sugars, ascorbic acid, phenolics,
flavonols and anthocyanins) compounds were quantified. The antioxidant activity was evaluated by radical scavenging activity,
reducing power and lipid peroxidation inhibition. The in vivo sample showed the highest antioxidant activity mainly due to its highest levels of hydrophilic compounds. Otherwise, in vitro samples, mainly clone A, gave the highest concentration in lipophilic compounds but a different profile when compared to
the in vivo sample. Clones A and B revealed a lack of β-carotene, β- and δ-tocopherols, a decrease in α-tocopherol, and an increase in
γ-tocopherol and clorophylls in comparison to the in vivo sample. In vitro culture might be useful to explore the plants potentialities for industrial applications, controlling environmental conditions
to produce higher amounts of some bioactive products. 相似文献
5.
Rosa Palmeri Cristina Restuccia Julieta Ines Monteleone Elisa Sperlinga Laura Siracusa Mauro Serafini Alberto Finamore Giovanni Spagna 《Plant foods for human nutrition (Dordrecht, Netherlands)》2017,72(2):211-218
Olive leaves represent a quantitatively significant by-product of agroindustry. They are rich in phenols, mainly oleuropein, which can be hydrolyzed into several bioactive compounds, including hydroxytyrosol. In this study, water extract from olive leaves ‘Biancolilla’ was analyzed for polyphenol profile, DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging activity and protective effect on differentiated Caco-2 cells. The efficacy of two enzymatic treatments in promoting the release of bioactive phenols was investigated: a) enzymatic extract from Wickerhamomyces anomalus, characterized by β-glucosidase and esterase activities; b) commercial β-glucosidase. Composition and bioactivity of the resulting extracts were compared. The results showed that the yeast-treated extract presented hydroxytyrosol content and DPPH radical scavenging activity comparable to those obtained using commercial β-glucosidase; however, it was showed the additional presence of hydroxycinnamic acids. In experiments on Caco-2 cells, the leaf extracts promoted the recovery of cell membrane barrier at different minimum effective concentrations. The high specificity of W. anomalus enzymatic extract may represent an effective tool for the release of bioactive phenols from olive by-products. 相似文献
6.
Lee BB Cha MR Kim SY Park E Park HR Lee SC 《Plant foods for human nutrition (Dordrecht, Netherlands)》2007,62(2):79-84
Organic solvent (methanol, ethanol, and acetone) extracts and water extracts of cherry (Prunus serrulata var. spontanea) blossoms were prepared, and antioxidant activities of the extracts were evaluated. Methanolic CBE (100 μg/ml) showed the
highest total phenol content (104.30 μM), radical scavenging activity (34.2%), and reducing power (0.391). The effect of CBE
on DNA damage induced by H2O2 in human leukocytes was evaluated by Comet assay. All CBE was a potent dose dependent inhibitor of DNA damage induced by
200 μM of H2O2, methanolic CBE showed the most strong inhibition activity. The methanolic CBE of 500 μg/ml showed 38.8% inhibition against
growth of human colon cancer cell line HT-29. These results indicated that cherry blossoms could provide valuable bioactive
materials. 相似文献
7.
Jae-Hee Park Yoo Kyoung Park Eunju Park 《Plant foods for human nutrition (Dordrecht, Netherlands)》2009,64(4):244-249
This study describes the antioxidant activities and antigenotoxic effects of garlic extracts prepared by different processing
methods. Aged-garlic extract (AGE) showed a significantly higher total phenolic content (562.6 ± 1.92 mg/100 g garlic acid
equivalents) than those of raw garlic extract (RGE) or heated garlic extract (HGE). The SC50 for DPPH RSA in HGE was significantly the highest at 2.1 mg/ml. The SC50 for SOD-like activity in garlic extracts was, in decreasing order, RGE (7.3 mg/ml) > AGE (8.5 mg/ml) > HGE (9.2 mg /ml).
The ED50 of AGE was the highest (19.3 μg/ml) regarding H2O2 induced DNA damage and its inhibition rate was 70.8%. The ED50 of RGE for 4-hydroxynonenal (a lipid peroxidation product) induced DNA damage was 38.6 μg/ml, followed by AGE > HGE. Although
the heat treatment of garlic tended to decrease the TPC and SOD-like activity and increased DPPH RSA, garlic, in general,
has significant antioxidant activity and protective effects against oxidative DNA damage regardless of processing method. 相似文献
8.
Tarhan L Kayali HA Urek RO 《Plant foods for human nutrition (Dordrecht, Netherlands)》2007,62(2):49-51
Total antioxidant capacities, 2,2-diphenyl-1-picrylhydrazyl (DPPH·), hydroxyl (HO·), scavenging activities, and total phenolic
values were determined in extracts of Cucurbita pepo L. female and male flowers. Powdered C. pepo L. samples were extracted in aqueous ethyl acetate (EA: W1, 17:3), ethanol (E), and water (W) by agitating in magnetic stirrer
for 80°C, 15 min and also by in aqueous ethyl acetate (EA: W2, 17:3) at 25°C, 15 min. DPPH·, HO· scavenging capacities and
total phenolic values of C. pepo L. female and male were higher in EA:W2 than in other extracts. In addition, all determined antioxidant capacities of female
were significantly higher than male. 相似文献
9.
Ezequiel?Grondona Gerardo?Gatti Abel?G.?López Leonardo?Rodolfo?Sánchez Virginia?Rivero Oscar?Pessah María?P.?Zunino Andrés?A.?Ponce
The aim of this study was to investigate the bioactivity of the essential oil isolated from Origanum vulgare L. (EOv). We analyzed the in vivo anti-inflammatory properties in a mouse-airway inflammation model and the in vitro antimicrobial activity, genotoxicity over the anaphase-telophase with the Allium cepa strain and its cytotoxicity/viability in A549 culture cells. In vivo, EOv modified the levels of tumor necrosis factor -α and viable activated macrophages and was capable to mitigate the effects of degradation of conjugated dienes. In vitro, EOv reduced the viability of cultured A549 cells as well as the mitotic index and a number of chromosomal aberrations; however, it did not change the number of phases. We found that EOv presents antimicrobial activity against different Gram (?) and (+) strains, measured by disc-diffusion test and confirmed with a more accurate method, the AutoCad software. We postulate that EOv presents antibacterial, antioxidant and chemopreventive properties and could be play an important role as bioprotector agent. 相似文献
10.
Polyphenolic Composition and Antioxidant Characteristics of Kumquat (<Emphasis Type="Italic">Fortunella margarita</Emphasis>) Peel Fractions 总被引:1,自引:1,他引:0
Engy Samih Sadek Dimitris P. Makris Panagiotis Kefalas 《Plant foods for human nutrition (Dordrecht, Netherlands)》2009,64(4):297-302
The polyphenolic composition of two Fortunella margarita (Nagami kumquat) specimens from Greece and Egypt was investigated employing fractionation by solvent partition and liquid
chromatography-mass spectrometry. The main groups of phenolics identified in the different fractions generated were C-glycosylated flavones, O-glycosylated flavones, C-glycosylated flavanones, O-glycosylated flavanones, flavonols, chalcones, phenolic acids and derivatives thereof. The antioxidant potency of the fractions
was assessed using two representative in vitro tests, including antiradical activity and hydroxyl free radical scavenging activity. It was revealed that the ethyl acetate
fractions from both specimens contained the higher polyphenol content and exhibited the better antioxidant characteristics.
The results indicated that F. margarita peels may be regarded as a rich source of potentially bioactive polyphenols. 相似文献
11.
S. Sreelatha P. R. Padma 《Plant foods for human nutrition (Dordrecht, Netherlands)》2009,64(4):303-311
Antioxidants play an important role in inhibiting and scavenging free radicals, thus providing protection to human against
infections and degenerative diseases. Current research is now directed towards natural antioxidants originated from plants
due to safe therapeutics. Moringa oleifera is used in Indian traditional medicine for a wide range of various ailments. To understand the mechanism of pharmacological
actions, antioxidant properties of the Moringa oleifera leaf extracts were tested in two stages of maturity using standard in vitro models. The successive aqueous extract of Moringa oleifera exhibited strong scavenging effect on 2, 2-diphenyl-2-picryl hydrazyl (DPPH) free radical, superoxide, nitric oxide radical
and inhibition of lipid per oxidation. The free radical scavenging effect of Moringa oleifera leaf extract was comparable with that of the reference antioxidants. The data obtained in the present study suggests that
the extracts of Moringa oleifera both mature and tender leaves have potent antioxidant activity against free radicals, prevent oxidative damage to major biomolecules
and afford significant protection against oxidative damage. 相似文献
12.
Tundis R Loizzo MR Menichini F Bonesi M Conforti F Statti G De Luca D de Cindio B Menichini F 《Plant foods for human nutrition (Dordrecht, Netherlands)》2011,66(3):261-269
The present study aimed to evaluate for the first time the phenols, flavonoids, carotenoids, capsaicin and dihydrocapsaicin
content and the antioxidant and hypoglycemic properties of Capsicum annuum var. acuminatum small and C. annuum var. cerasiferum air-dried fruits. The ethanol extract of C. annuum var. acuminatum small, characterized by the major content of total poliphenols, flavonoids, carotenoids and capsaicinoids, showed the highest
radical scavenging activity (IC50 of 152.9 μg/ml). On the contrary, C. annuum var. cerasiferum showed a significant antioxidant activity evaluated by the β-carotene bleaching test (IC50 of 3.1 μg/ml). The lipophilic fraction of both C. annuum var. acuminatum and C. annuum var. cerasiferum exhibited an interesting and selective inhibitory activity against α-amylase (IC50 of 6.9 and 20.1 μg/ml, respectively). 相似文献
13.
Custódio L Escapa AL Fernandes E Fajardo A Aligué R Alberício F Neng N Nogueira JM Romano A 《Plant foods for human nutrition (Dordrecht, Netherlands)》2011,66(1):78-84
This work aimed to evaluate the phytochemical content and to determine the antioxidant and cytotoxic activities of methanol
extracts of the carob tree (Ceratonia siliqua L.) germ flour. The extracts were rich in phenolic compounds, had considerable antioxidant activity, and reduced the viability
of cervical (HeLa) cancer cells. The chemical content and the biological activities of the extracts were significantly affected
by gender and cultivar. Female cultivar Galhosa had the highest levels of phenolic compounds, and the highest antioxidant
activity. Extracts from the hermaphrodite trees and from the female cultivars Galhosa and Costela/Canela exhibited the highest
cytotoxic activity. The most abundant compound was theophylline. The phenolic content was correlated to both antioxidant and
cytotoxic activities. Our findings provide new knowledge about the health implications of consuming food supplemented with
carob germ flour. 相似文献
14.
15.
López V Akerreta S Casanova E García-Mina JM Cavero RY Calvo MI 《Plant foods for human nutrition (Dordrecht, Netherlands)》2007,62(4):151-155
Eighty-eight extracts of different polarity obtained from 18 Lamiaceae medicinal and aromatic plants were screened for their
antioxidant and antifungal properties. A TLC analysis combined with two bioassays were used to detect these activities. In
most cases the highest radical scavenger capacity was detected in methanolic and aqueous extracts and polyphenols may be responsible.
The most interesting antioxidant activity was observed in polar extracts obtained from Lycopus europaeus, Melissa officinalis, Origanum vulgare subsp. virens and Lavandula latifolia. On the contrary, the best results for the antifungal test against Rhizopus stolonifer were produced by non-polar herbal extracts. Phlomis lychnitis, Salvia pratensis and Calamintha sylvatica caused the highest inhibition on Rhizopus growth. 相似文献
16.
Mariangela Marrelli Stefania La Grotteria Fabrizio Araniti Filomena Conforti 《Plant foods for human nutrition (Dordrecht, Netherlands)》2017,72(3):274-279
There is a great interest in the nutritional value of vegetables and fruits and how the habitat affects nutritive and biological properties. In vitro studies here reported were performed to evaluate the inhibitory activity of formulations from edible plant on pancreatic lipase. The aim of this study was also to evaluate the biovariability of L. comosa (L.) Parl. bulbs from Italy. The wild bulbs were compared with the same cultivated species that are commonly commercialized to identify samples with the best quality for a potential therapeutic application. Hydroalcoholic extract and polar fraction of wild bulbs showed a very important pancreatic lipase inhibitory activity, with IC50 values of 0.166 ± 0.005 and 0.153 ± 0.005 mg/mL, respectively. In order to characterize the extracts, gas chromatography associated with mass spectrometry (GC/MS) analysis was performed, revealing the predominance of palmitic acid. Phenolic and flavonoid composition was also evaluated. L. comosa extract obtained from wild bulbs demonstrated both antioxidant and anti-obesity activities that might be attributed to a wide range of present phenolic compounds. 相似文献
17.
Santos-Cervantes ME Ibarra-Zazueta ME Loarca-Piña G Paredes-López O Delgado-Vargas F 《Plant foods for human nutrition (Dordrecht, Netherlands)》2007,62(2):71-77
We report for the first time the antioxidant and antimutagenic activities of fractions from Randia echinocarpa fruit, which is a Rubiaceae plant native to Sinaloa, Mexico. This fruit has been traditionally used in the prevention or treatment of cancer, among other
diseases. The pulp of the fruit was sequentially extracted with solvents of different polarity (i.e. hexane, chloroform, methanol
and water). A high extraction yield was obtained with methanol (72.17% d.w.). The aqueous extract showed the highest content
of phenolics (2.27 mg/g as ferulic acid equivalents) and the highest antioxidant activity based on the β-carotene bleaching
method (486.15). The commercial antioxidant BHT was used as control (835.05). Antimutagenic activity of the aqueous extract
(0–500 μg/tube) was evaluated using the Salmonella microsuspension assay (YG1024 strain) and 1-NP as the mutagen (50 and 100 ng/tube). The aqueous extract was neither toxic
nor mutagenic and the percentage of inhibition on 1-NP mutagenicity was 32 and 53% at doses of 50 and 100 ng/tube, respectively.
The results of the double incubation assay suggest that the extract inhibited the mutagenicity of 1-NP by a combination of
desmutagenic and bioantimutagenic effects. 相似文献
18.
Milovanović V Radulović N Todorović Z Stanković M Stojanović G 《Plant foods for human nutrition (Dordrecht, Netherlands)》2007,62(3):113-119
The hydro-alcoholic extracts of five Equisetum species, E. arvense L., E. sylvaticum L., E. fluviatile L., E. palustre L. and E. telmateia Ehrh., growing-wild in Serbia were evaluated for their genotoxicity, antimicrobial activity, antioxidant capacity and the results
related to the total phenol content and HPLC flavonoid profiles. The total phenol content was 92–349 μmol expressed as equivalents
of chlorogenic acid per g of dried plant material. Main identified compounds were kaempferol-, quercetin- glycosides and caffeic
acid derivatives. E. telmateia extract showed the greatest antioxidant capacity. Almost all tested microorganisms demonstrated some degree of sensitivity
to the examined extracts. All tested extracts at 62.5 μg/ml showed higher incidence of micronucleus formation than in the
control sample. The obtained data allowed mutual comparison of examined species and their assessment as possible sources of
antioxidants, antimicrobials and/or genotoxic substances. 相似文献
19.
Pahua-Ramos ME Ortiz-Moreno A Chamorro-Cevallos G Hernández-Navarro MD Garduño-Siciliano L Necoechea-Mondragón H Hernández-Ortega M 《Plant foods for human nutrition (Dordrecht, Netherlands)》2012,67(1):10-16
Avocado seed contains elevated levels of phenolic compounds and exhibits antioxidant properties. We investigated the effect
of Avocado Seed Flour (ASF) on the lipid levels in mice on a hyperlipidemic diet. The concentration of phenols was determined
by high-performance liquid chromatography, antioxidant activity was evaluated using the Trolox equivalent antioxidant capacity
method, and dietary fiber was measured using the Association of Official Analytical Chemists (AOAC) method. The LD50 of ASF was determined using Lorke’s method and hypolipidemic activity was evaluated in a hypercholesterolemic model in mice.
Protocatechuic acid was the main phenolic compound found in ASF, followed by kaempferide and vanillic acid. The total phenolic
content in the methanolic extract of ASF was 292.00 ± 9.81 mg gallic acid equivalents/g seed dry weight and the antioxidant
activity resulted in 173.3 μmol Trolox equivalents/g DW. In addition, a high content of dietary fiber was found (34.8%). The
oral LD50 for ASF was 1767 mg/kg body weight, and treatment with ASF significantly reduced the levels of total cholesterol, LDL-C,
and prediction of the atherogenic index. Therefore, the antioxidant activity of phenolic compounds and dietary fiber in ASF
may be responsible for the hypocholesterolemic activity of ASF in a hyperlipidemic model of mice. 相似文献
20.
Hong Y Lin S Jiang Y Ashraf M 《Plant foods for human nutrition (Dordrecht, Netherlands)》2008,63(4):200-204
Eriobotrya plants are known to have significant amounts of phenolics and flavonoids, and exhibit a strong antioxidant activity. Experiments
were conducted to examine variation in the contents of total phenolics and flavonoids, and antioxidant activities in the leaves
of 11 Eriobotrya species (Tibet loquat, Daduhe loquat, Hengchun loquat, Taiwan loquat, Oak leaf loquat, Bengal loquat, Fragrant loquat, Guangxi
loquat, Obovate loquat, Big flower loquat, and common loquat, the last species include two materials, one is a cultivar ‘Zaozhong
6’, another is a wild tree). In these species, ‘Zaozhong 6′ loquat is a cultivar. The leaf extracts of ‘Tibet’, ‘Obovate’,
‘Taiwan’, ‘Bengal’ and ‘Hengchun’ loquats exhibited significantly higher contents of total flavonoids and total phenolics,
compared with those of other species. Of these 11 species, the highest contents of total phenolics and total flavonoids were
observed in ‘Tibet’ and ‘Obovatae’ loquats, respectively. The significantly stronger antioxidant abilities assessed by the
DPPH radical scavenging activity and reducing power were obtained in the leaf extracts of ‘Taiwan’, ‘Tibet’, ‘Bengal’, ‘Oak
leaf’, ‘Hengchun’ and ‘Obovate’ loquats, compared with the other species. In addition, significant correlations were found
between the contents of total phenolics or flavonoids and DPPH radical scavenging activity/reducing power. This work indicates
that the leaf extracts of the wild Eriobotrya species, ‘Tibet’, ‘Obovatae’, ‘Taiwan’, ‘Bengal’, ‘Oak leaf’ and ‘Hengchun’ loquats, exhibited significantly higher levels
of total phenolics and flavonoids, and significantly stronger antioxidant activities, compared with the cultivated species,
‘Zaozhong 6′ loquat, which suggests that these wild species have a better utilization value. 相似文献