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The distribution of 14C-acid-, 14C-alcohol-, and 14C-cyano-labeled deltamethrin and selected metabolites were followed in the liver, blood, cerebrum, cerebellum, and spinal cord after iv administration of a toxic, but nonlethal dose (1.75 mg/kg) to rats. Approximately 50% of the dose was cleared from the blood within 0.7–0.8 min, after which the rate of clearance decreased. 3-Phenoxybenzoic acid (PBacid) was isolated from the blood in vivo, and was also the major metabolite when 14C-alcohol-labeled deltamethrin was incubated with blood in vitro. Deltamethrin levels in the liver peaked at 7–10 nmol/g at 5 min and then decreased to 1 nmol/g by 30 min. In contrast, peak central nervous system levels of deltamethrin were achieved within 1 min (0.5 nmol/g), decreasing to 0.2 nmol/g at 15 min, and remaining stable until 60 min. peak levels of deltamethrin did not correspond to the severity of toxicity, although the levels of non-pentane-soluble radiolabel did appear to correlate with motor signs of toxicity. Experiments with brain homogenates, using in vivo concentrations of deltamethrin, failed to reproduce the pentane-unextractable radioactivity in vitro nor was any metabolism demonstrated.  相似文献   

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A total of 37 strains of Pseudomonas avellanae, P. syringae pv. theae and P.s. pv. actinidiae, including pathotype and reference strains, obtained from all the countries where these pathogens have been reported, were compared by means of ARDRA, repetitive PCR using ERIC, BOX and REP primer sets, whole-cell protein analysis, biochemical and nutritional tests, and pathogenicity tests. P. syringae pathovar type strains representing six genomospecies sensu Gardan et al. (1999), were also included for comparison in UPGMA cluster analysis of repetitive PCR data and SDS-PAGE of protein extracts. Among the 12 endonucleases used in ARDRA, only Tru 9I differentiated P. avellanae from P.s. pv. theae and P.s. pv. actinidiae. UPGMA cluster analysis of repetitive PCR genomic fingerprints showed 65% similarity between P.s. pv. theae and P. avellanae and 50% between the latter species and P.s. pv. actinidiae. Strains of P.s. pv. actinidiae could be grouped according to their geographic origin. Similar results were obtained with SDS-PAGE cluster analysis. PCR amplification using primers PAV 1 and PAV 22 that were developed to detect P. avellanae in apparently healthy and visibly infected hazelnut specimens yielded a band of 762bp from all strains of P. avellanae, P.s. pv. theae and P.s. pv. actinidiae. All strains lacked the syrB gene. Based on these data, we suggest that P.s. pv. actinidiae should be included in the genomospecies 8 together with P. avellanae and P.s. pv. theae. Selected biochemical and nutritional tests could differentiate these groups of strains. Pathogenicity tests clearly indicated that each group is specifically pathogenic only on the host plant species from which it was originally isolated.The author is staff member of the Istituto Sperimentale per la Patologia Vegetale, Roma, Italy temporarily assigned to ISF.  相似文献   

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Phytoparasitica - Plant-parasitic nematodes feed and reproduce in chickpea roots. Root-lesion nematodes are one of the most important biotic factors to limit chickpea production in the world. The...  相似文献   

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