猪瘟免疫失败剖析

猪瘟兔化疫苗使用过程中,发现不少免疫失败的惨痛教训。本文以作者自身经历,从免疫失败的调研、兔化疫苗的生产和毒价测定、免疫剂量、抗体水平与攻毒保护等关注热点问题作了回顾性比较剖析,对提高疫苗质量,清除免疫失败具有现实意义。     

五指山小型猪对猪病毒瘟的敏感性试验

本试验证明,２～４月龄猪瘟抗体阴性的五指山小型猪对猪瘟石门系病毒具有良好的敏感性。     

应用FA及PPA-ELISA技术对猪瘟的检测

用免疫荧光抗体诊断技术及单克隆抗体纯化酶联免疫吸附试验诊断技术对疑为猪瘟病毒感染猪进行了检测 ,重点阐述了 2种方法的技术原理和操作过程。通过用免疫荧光抗体诊断技术检测了 5份病料 ,其中有 2份为阳性 ,阳性率为 40 % ;用单克隆抗体纯化酶联免疫吸附试验诊断技术检测了猪瘟血清抗体 ,在 40头母猪血清样品中 ,猪瘟弱毒抗体效价 OD值较高 ,有 1 0 0 %的保护率 ,但是发现母猪群中有 1 0 %隐性猪瘟感染 ,其强毒抗体效价 OD值大于 0 .5,体内带有猪瘟病毒。结果及过程表明此两种诊断方法检测快速、鉴别准确、分辨率高     

新疆乌鲁木齐垦区猪瘟免疫程序的确定

多年来,新疆乌鲁木齐垦区没有一个科学合理的猪瘟免疫程序,免疫程序较为混乱,免疫效果亦不同。为探讨一种适合本地区的猪瘟免疫程序,有效预防猪瘟,而进行本试验。本试验是对仔猪猪瘟母源抗体的跟踪监测结果,对不同日龄仔猪免疫猪瘟疫苗,然后采集血样,检测猪瘟抗体效价。根据效价不断校正、最终确定为科学合理的猪瘟免疫程序,其免疫程序是:猪瘟的超前免疫,剂量1头份,根据抗体效价下降情况,35日龄进行二免,剂量4头份;仔猪常规免疫:一免30日龄、剂量2头份;二免50日龄、剂量4头份;生产母猪免疫:配种前(后)15d进行免疫,剂量4头份。应用此免疫程序的同时,严格遵守环境卫生消毒制度,再未发生猪瘟,抵御了猪瘟野毒的侵袋,提高了猪瘟防治水平,促进垦区生猪养殖向健康稳定的方向发展。     

Common diagnostic and clinical techniques for fish.

Fish medicine has common diagnostic and clinical procedures that are essential for disease diagnosis and patient management. With a few modifications for handling of the fish patient, these resemble procedures performed in most veterinary hospitals and clinics in our other companion species. The predictive value of these diagnostic methods is improving with the ever-increasing number of publications in fish medicine. Training in aquatic clinical procedures is offered at most large continuing education conferences and as specialty courses. These basic procedures are the important first steps toward improving the health of our aquatic companion animals.     

Ancillary diagnostic techniques for the lame patient.

The cause of lameness is usually identified through evaluation of signalment, history, and physical examination. Radiographic evaluation provides a definitive diagnosis in many cases; however, laboratory evaluation may prove useful in the characterization of certain conditions or in the prognostication of the condition. In some instances where the definitive diagnosis remains elusive, arthroscopic examination is a minimally invasive diagnostic tool that may be more affordable and available than computed tomography. This article reviews laboratory evaluation of the lame patient and arthroscopic evaluation of joint abnormalities.     

Green iguana nephrology: a review of diagnostic techniques.

There is still much to learn about renal physiology and pathophysiology in reptiles. In the case of the green iguana, initiating causes are often inferred from poor husbandry and nutrition, or extrapolated from histopathologic interpretations made late in the course of the disease, or at postmortem. The link between parathyroid hormone and renal disease in humans has been well documented and, given the high prevalence of clinical (and subclinical) secondary nutritional hyperparathyroidism in iguanas, this certainly warrants further investigation in saurians [31,32,34,35]. Apart from hyperparathyroidism, chronic water deprivation also appears to be a common historic factor. As a foliovore originating from the high humidity rain forests of central and South America, water recovery is not considered to be an adaptive stress in Iguana iguana, and therefore, renal anatomy and physiology are considered to be non-specialized compared to more arid or aquatic reptiles [21]. These arboreal lizards do not voluntarily drink from open water but instead imbibe rain or dew droplets from foliage [2]. Maintaining such a species in low relative humidity with a water bowl from which to drink is likely to both increase insensible water losses and interfere with normal water intake. Appropriate therapeutic decisions (including euthanasia) can only be made following an accurate diagnosis. To date, our diagnoses are based largely upon the structural evaluations of renal histopathology, and renal biopsy remains our most useful tool. However, in the future it may become possible to evaluate renal function by quantifying glomerular filtration rate, proximal tubular secretion, or functional renal mass. Glomerular filtration rate may be estimated by measuring the rate of glomerular clearance of substances from the blood (e.g., iohexol). Phenolsulphonphthalein assays may offer an insight into proximal tubule secretion and renal blood flow.     

2种猪瘟抗体检测技术的比较

猪瘟单抗 ELISA和正向间接血凝试验方法均可用于猪瘟抗体的检测 ,为了比较其效果 ,笔者应用这 2种方法对目前规模化猪场猪瘟免疫状况进行了猪瘟免疫抗体水平的检测 ,报告如下。1　材料与方法1 .1　材料1 .1 .1　猪瘟正向间接血凝诊断试剂　购自中国农业科学院兰州兽医研究所。1 .1 .2　猪瘟单抗 ELISA诊断试剂　购自中国兽医药品监察所。1 .1 .3　受检血清　采自新疆南北疆 3个规模化养殖场的不同日龄的猪血清 ,其中仔猪 ( 1 5～ 45日龄 )480份 ,生产母猪 5 2 0份 ,肥猪 ( 70～ 1 60日龄 ) 40 0份 ,置 4℃冰箱保存备用。 3个场猪瘟免疫…