Effect of Poultry Manure on Yield and Nutrient Composition of Sweet Basil (Ocimum basilicum L.)

Poultry manure is one of the best organic fertilizers available and is an extremely valuable resource, organic matter content, exchangeable cations, cation exchange capacity, and percent base saturation. Ocimum basilicum L. (sweet basil) is an essential oil producing crop used in culinary and fragrance applications. The objective of this present study was to evaluate the effects of organic poultry manure and conventional fertilization on plant growth, yield and inorganic matter content of sweet basil. In this context, the different doses of chicken and turkey manure (7.5, 10, 12.5, 15 t ha^?1) with a control (no manure) and conventional fertilization were applied with sowing. The highest fresh and dry weights were obtained from the plants grown with turkey and chicken manure at a rate of 10–12.5 t ha^?1. The most of the nutrient content varied significantly with organic poultry manure applications. Overall, basil plants fertilized with turkey manure at a rate of 10–12.5 t ha^?1 and 7.5 t ha^?1 chicken manure dose accumulated greater concentrations of potassium (K) and magnesium (Mg). The results from this study demonstrated that organic or conventional fertilizer can alter fresh or dry weight, and the nutrient content.     

Effectiveness of salicylic acid in mitigating salt-induced adverse effects on different physio-biochemical attributes in sweet basil (Ocimum basilicum L.)

The objective of this study was to determine the effects of foliar salicylic acid (SA) on salt tolerance of sweet basil seedlings by examining growth, photosynthetic activity, total osmoregulators, and mineral content under salinity. Salinity treatments were established by adding 0, 60, and 120 mM sodium chloride (NaCl) to a base nutrient solution. The addition of 60 and 120 mM NaCl inhibited the growth, photosynthetic activity, and nutrient uptake of sweet basil seedlings, and increased the electrolyte leakage and the plant contents of proline and Na. Sweet basil seedlings were treated with foliar SA application at different concentrations (0.0, 0.50, and 1.00 mM). Foliar applications of SA led to an increase in the growth, chlorophyll content, and gas exchange attributes. With regard to nutrient content, it can be inferred that foliar SA applications increased almost all nutrient content in leaves of sweet basil plants under salt stress. Generally, the greatest values were obtained from 1.00 mM SA application.     

Phenolics composition and antioxidant activity of sweet basil (Ocimum basilicum L.)

The antioxidant activity of a methanolic extract of Ocimum basilicum L. (sweet basil) was examined using different in vitro assay model systems. The crude extract was fractionated on a Sephadex LH-20 column, and six fractions were identified. The DPPH scavenging assay system and the oxidation of the soy phosphotidylcholin liposome model system were used to evaluate the antioxidant activity of each fraction. Fraction IV showed the strongest activity followed by fractions V and VI. Phenolic compounds responsible for the antioxidative activity of the fractions were characterized by atmospheric pressure chemical ionization liquid chromatography-mass spectrometry. The major antioxidant compound in fraction IV was confirmed as rosmarinic acid by (1)H NMR and characteristic fragmentations in the mass spectrum. Moreover, the native of antioxidant activity of rosmarinic acid in the liposome system was examined. The results showed that one rosmarinic acid can capture 1.52 radicals, and furthermore, the existence of a synergistic effect between alpha-tocopherol and rosmarinic acid was revealed.     

Effects of nitrogen fertilization on the phenolic composition and antioxidant properties of basil (Ocimum basilicum L.)

Many herbs and spices have been shown to contain high levels of polyphenolic compounds with potent antioxidant properties. In the present study, we explore how nutrient availability, specifically nitrogen fertilization, affects the production of polyphenolic compounds in three cultivars (Dark Opal, Genovese, and Sweet Thai) of the culinary herb, basil ( Ocimum basilicum L.). Nitrogen fertilization was found to have a significant effect on total phenolic levels in Dark Opal ( p < 0.001) and Genovese ( p < 0.001) basil with statistically higher phenolic contents observed when nutrient availability was limited at the lowest (0.1 mM) applied nitrogen treatment. Similarly, basil treated at the lowest nitrogen fertilization level generally contained significantly higher rosmarinic ( p = 0.001) and caffeic ( p = 0.001) acid concentrations than basil treated at other nitrogen levels. Nitrogen fertilization also affected antioxidant activity ( p = 0.002) with basil treated at the highest applied nitrogen level, 5.0 mM, exhibiting lower antioxidant activity than all other nitrogen treatments. The anthocyanin content of Dark Opal basil was not affected by applied nitrogen level, but anthocyanin concentrations were significantly impacted by growing season ( p = 0.001). Basil cultivar was also determined to have a statistically significant effect on total phenolic levels, rosmarinic and caffeic acid concentrations, and antioxidant activities.     

Effect of methyl jasmonate on secondary metabolites of sweet basil (Ocimum basilicum L.)

The effect of methyl jasmonate (MeJA) in terms of its induction of inherent bioactive chemicals in sweet basil (Ocimum basilicum L.) was evaluated after MeJA was sprayed on healthy basil plants. The total phenolic content of the sweet basil significantly increased after 0.1 and 0.5 mM MeJA treatments compared with the control not subjected to MeJA. Two phenolic compounds, rosmarinic acid (RA) and caffeic acid (CA), were identified as strong antioxidant constituents of the sweet basil. Their amounts also significantly increased after the MeJA treatment. In addition, eugenol and linalool increased 56 and 43%, respectively, by the 0.5 mM MeJA treatment. Due to the accumulation of RA, CA, and eugenol, which possess strong 2,2-diphenyl-1-picrylhydrazyl (DPPH*) free radical scavenging activities, the antioxidant activity of the sweet basil extract was 2.3-fold greater than that of the control after the 0.5 mM MeJA treatment. In the DPPH* assay, the EC50 values of RA, CA, and eugenol were determined as 23, 46, and 59 microM, respectively, which indicated they were 6-, 3-, and 2.4-fold more efficient than BHT (140 microM). Besides, an unidentified HPLC peak in the methanolic extract of the sweet basil was 4.3-fold higher than that of the control after the 0.5 mM MeJA treatment.     

Effect of chitosan on the biological properties of sweet basil (Ocimum basilicum L.)

The effect of the treatment of chitosan at various concentrations (0.01%, 0.05%, 0.1%, 0.5%, and 1%) upon sweet basil (Ocimum basilicum L.) before seeding and transplanting was investigated in aspects of the amount of phenolic and terpenic compounds, antioxidant activity, and growth of the basil, as well as the phenylalanine ammonia lyase (PAL) activity. The total amount of the phenolic and terpenic compounds increased after the chitosan treatment. Especially, the amounts of rosmarinic acid (RA) and eugenol increased 2.5 times and 2 times, respectively, by 0.1% and 0.5% chitosan treatment. Due to the significant induction of phenolic compounds, especially RA, the corresponding antioxidant activity assayed by the DPPH (2,2-diphenyl-1-picrylhydrazyl) free radical scavenging test increased at least 3.5-fold. Also, the activity of PAL, a key regulatory enzyme for the phenylpropanoid pathway, increased 32 times by 0.5% chitosan solution. Moreover, after the elicitor chitosan treatment, the growth in terms of the weight and height of the sweet basil significantly increased about 17% and 12%, respectively. Our study demonstrates that an elicitor such as chitosan can effectively induce phytochemicals in plants, which might be another alternative and effective means instead of genetic modification.     

Yield and oil composition of 38 basil (Ocimum basilicum L.) accessions grown in Mississippi

A field experiment was conducted to assess yield, oil content, and composition of 38 genotypes of sweet basil ( Ocimum basilicum L.). Overall, biomass yields were high and comparable to those reported in the literature. However, basil genotypes differed significantly with respect to oil content and composition. Oil content of the tested accessions varied from 0.07% to 1.92% in dry herbage. On the basis of the oil composition, basil accessions were divided into seven groups: (1) high-linalool chemotype [19-73% (-)-linalool], (2) linalool-eugenol chemotype [six chemotypes with 28-66% (-)-linalool and 5-29% eugenol], (3) methyl chavicol chemotype [six accessions with 20-72% methyl chavicol and no (-)-linalool], (4) methyl chavicol-linalool chemotype [six accessions with 8-29% methyl chavicol and 8-53% (-)-linalool], (5) methyl eugenol-linalool chemotype [two accessions with 37% and 91% methyl eugenol and 60% and 15% (-)-linalool], (6) methyl cinnamate-linalool chemotype [one accession with 9.7% methyl cinnamate and 31% (-)-linalool], and (7) bergamotene chemotype [one accession with bergamotene as major constituent, 5% eucalyptol, and <1% (-)-linalool]. Our results demonstrated that basil could be a viable essential oil crop in Mississippi. The availability of various chemotypes offers the opportunity for production of basil to meet the market requirements of specific basil oils or individual compounds such as (-)-linalool, eugenol, methyl chavicol, methyl cinnamate, or methyl eugenol.     

Amelioration of salinity stress in different basil (Ocimum basilicum L.) varieties by vesicular-arbuscular mycorrhizal fungi

Although the salt resistance mechanisms in plants have received much consideration for many years, varieties’ differences affecting salt resistance are still unsettled. Within the Ocimum genus there occur about 200 species in different varieties and forms. A pot experiment was performed to better understand salt stress responses in crop plants; we compared the impacts of salinity stress on growth and physio-biochemical characteristics in three varieties of basil (Ocimum basilicum) var. odoratus, O. b. var. alba and O. b. var. purpurascens) grown under four levels of salinity stress (0, 50, 100, and 150?mM NaCl) with mycorrhiza (Glomus clarum Nicol. &; Schenck) or without. Results showed significant differences within salinity treatments in all cultivars studied. In this study, the biomass production and physio-biochemical parameters of all cultivars reduced with raised salinity levels except concentration of reducing sugars, sodium, and proline at 150 mM of NaCl, only the variety ‘purpurascens’ didn't show reduction and observed resistant against severe salinity. The colonization of arbuscular mycorrhiza fungi enhanced the biomass production and accumulation of nutrients, reducing sugars, total soluble carbohydrates, photosynthetic pigments, proline, and protein by reducing Na. This study should help understand the function of AMF fungi in basil cultivars’ tolerance to salinity stress.     

Potassium fertilization and soil diagnostic criteria for forage corn (Zea mays L.) production contributing to lower potassium input in regional fertilizer recommendation

Abstract

Effective soil diagnostic criteria for exchangeable potassium (Ex-K) combined with inorganic potassium (K) application rates were developed to lower K input in forage corn (Zea mays L.) production using experimental fields with different application rates and histories of cattle manure compost. Two corn varieties, ‘Cecilia’ as a low K uptake variety and ‘Yumechikara’ as a high K uptake variety, were selected from among 20 varieties and tested to make diagnostic criteria for K fertilization applicable to varieties with different K uptakes. The K uptakes increased from 96 to 303 kg K ha^?1 for ‘Cecilia’ and from 123 to 411 kg K ha^?1 for ‘Yumechikara’ with increasing Ex-K content on a dry soil basis from 0.11 to 0.92 g kg^?1 with no inorganic K fertilizer application. The K uptake by corn for achieving the target dry matter yield of 18 Mg ha^?1 was estimated to be approximately 200 kg K ha^?1 in common between the two varieties. Yields of both varieties achieved the target yield at an Ex-K content of approximately 0.30 g kg^?1 with no K fertilization, although ‘Yumechikara’ reached the target yield at a lower Ex-K content. At the low Ex-K content of 0.1 g kg^?1, inorganic K fertilizer application at 83 kg K ha^?1 was needed to gain the target yield, and apparent K recovery rate for K fertilizer was calculated to be 70% for both varieties. The K uptakes for gaining the target yield by the K fertilization were lower than that by soil K supply. Based on these results, diagnostic criteria of Ex-K and inorganic K application rates were set up as follows: at an Ex-K content of < 0.15 g kg^?1, inorganic K fertilizer is applied at 83 kg K ha^?1 (100 kg ha^?1 as potassium oxide (K₂O) equivalent); at an Ex-K content of 0.15–0.30 g kg^?1, the application rate is reduced to 33 kg K ha^?1 (40 kg K₂O ha^?1); at an Ex-K content of ≥ 0.30 g kg^?1, inorganic K fertilizer is not applied because of sufficient K in the soil. Additionally, we propose that cattle manure compost be used to supplement soil K fertility.     

Light reflected from colored mulches affects aroma and phenol content of sweet basil (Ocimum basilicum L.) leaves

Basil (Ocimum basilicum L.) is an herb the leaves of which are used to add a distinct aroma and flavor to food. It was hypothesized that the size and chemical composition of sun-grown basil leaves could be influenced by the color of light reflected from the soil surface and by the action of the reflected light through the natural growth regulatory system within the growing plants. Leaf morphology, aroma compounds, and soluble phenolics were compared in basil that had been grown over six colors of polyethylene row covers. Altering the ratios of blue, red, and far-red light reflected to growing plants influenced both leaf morphology and chemistry. Leaves developing over red surfaces had greater area, moisture percentage (succulence), and fresh weight than those developing over black surfaces. Basil grown over yellow and green surfaces produced significantly higher concentrations of aroma compounds than did basil grown over white and blue covers. Leaves grown over yellow and green mulches also contained significantly higher concentrations of phenolics than those grown over the other colors. Clearly, the wavelengths (color) of light reflected to growing basil plants affected leaf size, aroma, and concentrations of soluble phenolics, some of which are antioxidants.     

Chemical characterization of basil (Ocimum basilicum L.) found in local accessions and used in traditional medicines in Iran

Ocimum species are used in traditional Iranian medicine, as a culinary herb, and as a well-known source of flavoring principles. Horticultural characteristics, including quantitative and qualitative traits along with the chemical variation of phenolic acids, of 23 accessions of basil (Ocimum basilicum L.) from Iran were studied. Morphological studies of accessions showed a high level of variability in recorded traits. Quantification of phenolic acids was determined using high-performance liquid chromatography and showed drastic variations between accessions. Chemical studies revealed that rosmarinic acid is the predominant phenolic acid present in both flower and leaf tissues. Unusual basil accessions were identified that can serve as genetic sources of phenolic acids for crop improvement.     

Aroma content of fresh basil (Ocimum basilicum L.) leaves is affected by light reflected from colored mulches

Sweet basil (Ocimum basilicum L.) is an herb that is used to add a distinct aroma and flavor to food. Volatile compounds emitted from fully expanded fresh leaves grown in drip-irrigated plots that were covered with six colors of mulch were compared. The colors reflected a range of photosynthetic photon flux, far-red, red, and blue light from the soil surface to developing leaves. Our objective was to determine whether reflection from the different colors could influence concentrations of volatile compounds emitted from the fresh leaves. Volatile compounds were isolated by headspace sampling and quantified by gas chromatography. Twenty-six compounds were identified, of which the terpenoids linalool and 1,8-cineole comprised more than 50% of the total yield. Concentrations of volatile compounds from leaves that developed over green, blue, yellow, white, and red mulches followed the same patterns as they did for air-dried leaves of the same cultivar. However, the concentration of volatile compounds from fresh leaves was about 50-fold higher than those found in the previous study of air-dried leaves.     

Flavor volatiles and physical properties of vacuum-microwave- and air-dried sweet basil (Ocimum basilicum L.).

Basil (Ocimum basilicum L.) was dried using conventional hot air or the recently developed vacuum-microwave dryers. The effect of the drying method on the relative abundance of major flavor volatiles, rehydration rate, color, and structural integrity of the plant was evaluated. Dynamic headspace analysis of volatiles present in fresh or dried basil revealed that linalool and methylchavicol (estragole) were the two major headspace volatile compounds of the plant sample. Vacuum-microwave dehydrated basil yielded approximately 2.5 times the linalool and 1.5 times the methylchavicol of the air-dried samples. Furthermore, the vacuum-microwave-treated samples yielded more volatiles than fresh basil, due to chemical reactions during drying. Air-dried samples of basil had darker and fewer green hues than those prepared by vacuum microwave. Vacuum-microwave-dried samples had a higher rehydration rate, whereas the potential of the plant material to rehydrate was hindered in air-dried samples. This is likely attributed to the dramatic and pronounced structural collapse of the air-dried cells as revealed by the scanning electron microscope.     

长期施肥对栗褐土钾素含量的影响

在栗褐土上进行的长期定位施肥试验结果表明,18年后不同施肥处理土壤全钾含量无显著差异,而土壤速效钾含量和缓效钾含量则产生了极显著差异。就表层土壤而言,无外源钾补充的3个处理(NP、N、CK),土壤速效钾含量较低,在78~90 mg.kg-1之间,已接近中等水平的下限,施低量有机肥的3个处理(M1、M1N、M1NP),土壤速效钾含量为150~185 mg.kg-1,施高量有机肥的2个处理(M2NP、M2N),土壤速效钾含量达240mg.kg-1以上。不同处理对土壤缓效钾含量的影响与土壤速效钾含量基本一致,施高量有机肥的处理>施低量有机肥的处理>没施有机肥的处理,但处理间土壤缓效钾的变化幅度明显低于土壤速效钾含量的变化幅度。不同处理对20~40 cm土壤速效钾和缓效钾含量也产生了一定影响,变化规律与耕层相似,但没有耕层明显。     

Soybean (Glycine max (L.) Merr.) yield and disease incidence with potassium fertilization

Abstract

Six cultivars and three lines of soybean (Glycine max (L.) Merr.) were planted at two locations in Delaware in 1974 and 1975. Potassium fertilizer (KCl) at 0, 56, or 223 kg/ha actual K was added to the soil and the plants were evaluated for seed yield and incidence of gray‐moldy and purple‐stained seed.

Potassium application was related to a decrease in yield in one year at one location but had no significant effect in the other year or at the other location. Cultivars differed in yield over the two years, with Kent, Cutler 71, and York having the most consistent yield in this study. The pathogen incidence was not affected by potassium application in contrast to earlier studies with soybeans grown in aluminum cylinders with a partially restricted soil volume. In this study, gray‐moldy and purple‐stain was highest in short season cultivars and higher at Georgetown than at Newark.

Use of soybean cultivars resistant to diseases as well as maintaining adequate soil potassium levels should reduce high levels of disease.     

Effect of cultivar on the protection of cardiomyocytes from oxidative stress by essential oils and aqueous extracts of basil (Ocimum basilicum L.)

Notwithstanding the wide range of biological and pharmacological activities reported for sweet basil (Ocimum basilicum L.), many discrepancies are still present in the evaluation of its health-promoting properties. These discordances could be at least in part due to insufficient details of qualitative and quantitative composition, connected to the ample variability of this species. Furthermore, many investigations have been carried out in vitro, with few data available on the effectiveness in biological systems. In this study, the protective effect of essential oils and water-soluble extracts derived from three different cultivars of sweet basil has been evaluated in cultured cardiomyocytes. To verify the effectiveness of supplemented oils/extracts in counteracting oxidative damage, cardiomyocytes were stressed by the addition of hydrogen peroxide. The results indicate that (a) in vitro antioxidant activity is not predictive of biological activity and (b) basil can yield extracts with substantially different protective effects, in relation to composition and extraction techniques. Variation among different cultivars has also been detected.     

长期定位施肥对非石灰性潮土钾素状况的影响

利用22年肥料长期定位试验,研究施肥对土壤钾素状况的影响。结果表明,1978年到2000年,土壤钾素收支平衡的状况为:氮钾肥处理钾盈余57.72kg/hm2,氮磷钾肥处理钾亏缺192.90kg/hm2。以氮钾肥处理提高土壤中水溶性钾、非特殊吸附钾、特殊吸附钾、矿物钾最多,依次分别比对照提高5.49倍、1.13倍、2.47倍和1.11倍。氮磷钾肥和氮钾肥处理在土壤中积累各形态钾总量分别占施钾量的19.18%、20.01%。氮钾处理和氮磷钾处理土壤速效钾和土壤缓效钾含量高,在土壤剖面中分布以0—20cm含量高,20cm以下逐层降低。用X-射线衍射测定长期施钾和不施钾的土壤粘土矿物组成为:氮磷钾处理土壤粘土矿物中蒙脱石峰值高;而氮磷处理土壤粘土矿物中蛭石峰值高。     

Potassium efficiency of safflower (Carthamus tinctorius L.) and sunflower (Helianthus annuus L.)

The oil crop safflower may have a certain production potential under low‐input conditions (organic farming, developing countries), where the putatively low nutrient requirement is highly welcomed. However, current knowledge regarding the nutrient use efficiency of safflower as compared to similar oil crops is limited. It was thus the aim of this study to determine the potassium (K) use efficiency of safflower (Carthamus tinctorius L.) as compared to sunflower (Helianthus annuus L.). Safflower and sunflower were cultivated with increasing K supply in a mixture of equal volumes of sand, nutrient‐poor limed soil, and perlite in 5 L Mitscherlich pots. Both species responded strongly to increasing K supply with respect to growth and yield. Safflower out‐yielded sunflower at low K supply, while at high K level, the opposite was observed. Both species accumulated similar amounts of K in shoots at low K supply. Only at extremely low K supply, safflower took up more K than sunflower. However, achene yield of sunflower exceeded that of safflower at optimal and high K supply. Safflower utilized absorbed K more efficiently than sunflower to produce achene yield at suboptimal K supply in terms of both efficiency ratio and utilization index. The efficiency of a crop to use supplied or accumulated K for dry‐matter and achene production was interpreted in terms of Michaelis‐Menten kinetics, specifically addressing the shape of the yield response curve. Indeed, the efficiency of safflower to use K for growth and yield, analogue to a low K_m in enzyme kinetics, was higher than in sunflower, while the K supply or K accumulation required to initiate yield formation in safflower was significantly lower. Similarly, safflower had a lower external K requirement for achene yield than sunflower at low and optimal K supplies. It can be concluded that safflower represents a low‐input crop and outperforms sunflower on soils low in available K. The data analysis also reveals that using just one efficiency indicator is usually not sufficient to adequately describe the K efficiency of the crop under consideration.     

Variation in essential oil composition within individual leaves of sweet basil (Ocimum basilicum L.) is more affected by leaf position than by leaf age

The aroma in sweet basil is a factor affecting the commercial value of the crop. In previous studies leaf age was considered to be a factor that influences the composition of essential oil (EO). In this study it was hypothesized that a single observation of the EO content in leaves from different positions on the main stem (young vs old) could predict the developmental changes in the plant during its life cycle. Plants harvested at week 16 demonstrated an exponential increase (R(2) = 0.92) in EO concentration in leaves on the main stem and lateral shoots, indicating higher EO concentrations in younger than in older leaves. Eugenol and methyleugenol predominated (28-77%) in the extract. Eugenol levels were higher in younger leaves (～53%), and methyl-eugenol levels predominated in older leaves (～68%). Linalool was lower in mature leaves than in younger leaves. This suggested that eugenol converted into methyleugenol and linalool decreased as leaf mature. However, in weekly monitored plants, the levels of these compounds in the EO had limited variation in the maturing leaf regardless of its position on the stem. This proposed that the EO composition in an individual leaf is mostly affected by the leaf position on the stem and not by its maturation process. Because leaf position is related to plant development, it is probable that the plant's physiological age at the time of leaf formation from the primordial tissue is the factor affecting the EO composition. It was concluded that interpretation of scientific observations should be carried out with caution and that hypotheses should be tested utilizing multifaceted approaches.