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1.
Sir, — Andrews’(2) checklist of helminth parasites of wild ruminants in New Zealand recorded the presence of 10 species of gastrointestinal nematodes in chamois (Rupicapra rupicapra rupicapra L.) from “Central South Island”. In October 1978 we examined 28 freshly-shot chamois cadavers from the Harper-Avoca watershed in the headwaters of the Rakaia River. Six of the nematode species reported by Andrews were found again; viz. Ostertagia ostertagi, O. circumcincta, O. trifurcata, Nematodirus filicollis, Oesophagostomum venulosum and Trichuris ovis. The 4 species found by Andrews that were not recovered by us from the animals we examined were: Trichostrongylus axei, T. vitrinus, Spiculotragia spiculoptera and S. asymmetrica. On the other hand we found 4 helminths not encountered by Andrews: 3 nematodes: Nematodirus spathiger (Railliet, 1896), N. abnormalis (May, 1920), and Chabertia ovina (Fabricius, 1794), and 7 specimens of the cestode Moniezia expansa (Rud., 1805). Nematodirus spathiger was the most commonly encountered Nematodirus species, but generally the numbers of all 3 species encountered were low as might be expected in a sample of adult and almost year-old animals. It is probable that most of the adults had acquired some measure of immunity(3), and the most susceptible group, the kids, would not be born until late November. Because peptic digestion was not used to recover juveniles from the intestinal submucosa, only small numbers of adults were obtained. 相似文献
2.
J Boomker I G Horak K M MacIvor 《The Onderstepoort journal of veterinary research》1989,56(3):165-172
Helminth parasites were collected from 13 grysbok, Raphicerus melanotis, 12 common duikers, Sylvicapra grimmia, 24 Angora goats and 24 Boer goats killed on a farm in Valley Bushveld in the eastern Cape Province. Nematodes belonging to 9 species and a further 2 genera and cestodes belonging to 1 species and a further genus were recovered from the grysbok. Of these, worms of the genus Skrjabinema, Trichostrongylus rugatus, Trichostrongylus pietersei and Nematodirus spathiger were the most prevalent and the most numerous. An amended list of the helminths of grysbok is included. Seven nematodes, identified to species and 1 identified to genus were recovered from the common duikers. N. spathiger and Setaria scalprum were the most prevalent, while N. spathiger and Trichostrongylus axei were the most abundant. The Angora goats were infested with at least 10 nematode species and 2 cestodes and the Boer goats with at least 14 nematode species. With the exception of Trichostrongylus pietersei, the Boer goats harboured more nematodes of each species than did the Angora goats. Nematodirus spp. and worms of the genus Skrjabinema were most numerous in Angora goats, and Nematodirus spp. and Teladorsagia circumcincta in the Boer goats. No clear pattern of seasonal abundance was evident for any of the helmith species in the various hosts. 相似文献
3.
Sixty-five gazelles, Gazella subgutturosa, of different ages and sexes, from different National Parks and Protected Regions of Iran, were examined over a period of 2 years, for gastro-intestinal nematodes. All of the examined animals were infected with one or more nematode species. The following species were found: Marshallagia marshalli, Camelostrongylus mentulatus, Ostertagia occidentalis, O. circumcincta, Trichostrongylus colubriformis, T. vitrinus, Nematodirus gazellae, N. oiratianus, N. spathiger, N. archari, N. abnormalis, N. filicollis, N. sugatini, Nematodirella cameli, N. longissimespiculata, N. antilocaprae, N. gazelli, Skrjabinema ovis, Trichuris ovis, T. gazellae, T. skrjabini and T. discolor. Among the species found, Marshallagia marshalli, Nematodirus spp. and Nematodirella longissimespiculata were the most prevalent helminths. 相似文献
4.
Parasites were collected from 160 Angora goats and kids on the Jansenville Experimental Farm, Eastern Cape Province. Six nematodes were identified to species level and three to generic level. Nematodirus spathiger was the most numerous of the economically important nematodes recovered. It was always present, and fourth stage larvae were collected from untreated goats from August to March, while animals used as tracers also picked up most infection from August to March. Kids born on the farm during October acquired their first nematode infections between 2 and 3 months of age and the intensity of infection increased erratically thereafter to reach a plateau once the kids were 14 months of age. Eight of the nine kids between 3 and 5 months of age and examined between January and March were infected with Moniezia expansa. Five ixodid tick species were collected from the goats of which Rhipicephalus glabroscutatum was the most numerous and prevalent. Its immature stages were present mainly from March to September and adults from July to January. The goats also harboured the biting louse Damalinia limbata and the sucking louse Linognathus africanus. The greatest intensity of infestation with L. africanus occurred on the kids during the first few months of their lives. The larvae of the nasal bot fly, Oestrus ovis were present in kids at 1 month of age, and infestation took place mainly from spring to late summer. 相似文献
5.
Summary. The species of trichostrongyloid nematodes present in 376 sheep from different agricultural regions of the state of South Australia were determined.
Parasites encountered in the abomasum were Teladorsagia circumcincta, T. trifurcata, T. davtiani, Camelostrongylus mentulatus, Haemonchus contortus and Trichostrongylus axel. T. circumcincta was the species most commonly encountered, but C. mentulatus was widespread in arid areas. H. contortus was present in a small number of sheep only, mainly in southern areas.
In the small intestine, Trichostrongylus vitrinus predominated in the wetter, southern districts, and T. rugatus in dry, northern areas. T. colubriformis was common, but was rarely dominant. T. probolurus was found in one sheep. Nematodirus filicollis was restricted to wetter, southern areas, while N. spathiger and N. abnormalis were widespread and predominated in dry areas. N. helvetianus was found once. Cooperia species were uncommon; species encountered were C. oncophora, C. surnabada and C. pectinata.
Thanks are due to P. M. Bonnin, E. Moore, S. M. Fitzsimons, F. M. and R. R. Martin and M. G. O'Callaghan for excellent technical assistance, and to members of the South Australian Department of Agriculture, particularly the staff of the Northern Region, for collecting material or helping with the collection. 相似文献
Parasites encountered in the abomasum were Teladorsagia circumcincta, T. trifurcata, T. davtiani, Camelostrongylus mentulatus, Haemonchus contortus and Trichostrongylus axel. T. circumcincta was the species most commonly encountered, but C. mentulatus was widespread in arid areas. H. contortus was present in a small number of sheep only, mainly in southern areas.
In the small intestine, Trichostrongylus vitrinus predominated in the wetter, southern districts, and T. rugatus in dry, northern areas. T. colubriformis was common, but was rarely dominant. T. probolurus was found in one sheep. Nematodirus filicollis was restricted to wetter, southern areas, while N. spathiger and N. abnormalis were widespread and predominated in dry areas. N. helvetianus was found once. Cooperia species were uncommon; species encountered were C. oncophora, C. surnabada and C. pectinata.
Thanks are due to P. M. Bonnin, E. Moore, S. M. Fitzsimons, F. M. and R. R. Martin and M. G. O'Callaghan for excellent technical assistance, and to members of the South Australian Department of Agriculture, particularly the staff of the Northern Region, for collecting material or helping with the collection. 相似文献
6.
The number and species of helminth parasites from three gemsbok (Oryx gazella) were recorded, and their faecal nematode egg counts and the level of pasture contamination determined. Six nematode genera were recovered and four species identified, of which Trichostrongylus rugatus was the most prevalent. Other nematode species recovered were Cooperia sp., Agriostomum sp., Haemonchus contortus, Nematodirus spathiger and Ostertagia ostertagi. None of the worms were present in all animals studied, and no new host associations were found. Cysticerci were recovered from the mesenteries of one gemsbok and a further two unidentifiable helminths were recovered from the abomasum and the kidney fat layer of another antelope. 相似文献
7.
The anthelmintic efficacy of netobimin against naturally acquired gastrointestinal nematodes in sheep 总被引:1,自引:0,他引:1
L S Richards G L Zimmerman E P Hoberg D J Schons S W Dawley 《Veterinary parasitology》1987,26(1-2):87-94
The broad-spectrum anthelmintic efficacy of netobimin (SCH 32481, Schering Corporation) was evaluated using 30 cross-bred spring lambs with naturally acquired infections of gastrointestinal nematodes. Three groups of 10 animals each were allotted into either control (given a tap water drench as a placebo) or 7.5 and 20 mg kg-1 dosage groups (given the netobimin as an oral drench). Seven to fourteen days post-treatment, animals were necropsied and nematodes recovered by standard techniques. Examination of fecal samples taken on dates of necropsy showed median egg production was reduced in treated animals (61.98% with 7.5 mg kg-1 and 100% with 20 mg kg-1). The compound was highly effective in removal of adult nematodes representing a number of genera and species of trichostrongyloids at the 7.5 and 20 mg kg-1 dose levels (shown, respectively, below). These included Ostertagia spp., with O. circumcincta, O. trifurcata, O. ostertagi and Teladorsagia davtiani (96.20%; 100%), Trichostrongylus spp., with T. axei, T. vitrinus and T. colubriformis (100%; 98.72%), Nematodirus spp., with N. spathiger, N. filicollis and N. battus (100% both levels) and Haemonchus contortus (100% both levels). High efficacies against other species of nematodes (at both dose levels) were not statistically significant (Cooperia spp., Chabertia ovina and Oesophagostomum venulosum). At 20 mg kg-1, netobimin significantly reduced populations of early and late fourth stage larvae of Ostertagia spp. by 100%. The overall efficacy (all life stages included) was 90.16% at 7.5 mg kg-1 and 98.77% at 20 mg kg-1 dose levels. No adverse reactions or signs of toxicosis were observed. 相似文献
8.
J A Van Wyk H M Gerber W P Van Aardt 《The Onderstepoort journal of veterinary research》1977,44(3):173-194
Exsheathed infective larvae (L 3) of 19 species of nematodes were tested for infectivity in either sheep or cattle after they had been frozen in 0,9% NaCl solution, stored for a relatively short time in the gas phase of liquid nitrogen and subsequently thawed. In addition, 13 of these species were tested after similar storage for up to 18 months. In sheep, Haemonchus contortus, Ostertagia circumcincta, Trichostrongylus axei, Trichostrongylus colubriformis, Nematodirus spathiger and Oesophagostomum columbianum were viable after 2 years of cryopreservation, a mean of greater than 90% of the L 3 being alive when thawed after this period. Similar results were obtained with Chabertia ovina L 3 after 18 months and with Marshallagia marshalli, Trichostrongylus falculatus and Dictyocaulus filaria, after a short period of freezing. On the other hand, Gaigeria pachyscelis and Strongyloides papillosus survived freezing for up to 7 months but neither was viable at the end of this period, nor was exsheathed G. pachyscelis viable without freezing. Most of these infestations were established by inoculating the infective larvae into the abomasum and/or duodenum. M. marshalli, T. falculatus and C. ovina also proved infective after oral dosing. D. filaria, the only other species tested by this route, was not infective when dosed per os after thawing. The infective larvae of the bovine nematodes, Haemonchus placei, Ostertagia ostertagi, Nematodirus helvetianus, Oesophagostomum radiatum, Cooperia pectinata and Cooperia punctata survived freezing for a mean of 26 months, greater than 90% being alive on thawing, but infectivity was generally lower than with the same genera in sheep. Even when not frozen, exsheathed Bunostomum phlebotomum was non-infective. When Cooperia spp. after thawing were tested for infectivity by the oral route, more worms developed in one calf infested orally than in another infested by inoculation into the duodenum. Ova of H. contortus, M. marshalli, O. circumcincta, T. colubriformis, T. falculatus, N. spathiger, C. ovina, H. placei, O. ostertagi, Cooperia spp. and N. helvetianus were recovered from the faeces of animals infested with cryopreserved L 3. No ova of O. columbianum or O. radiatum were recovered from faeces, because differential larval counts were performed before they were patent. Nevertheless, gravid females were obtained post-mortem. Frozen L 3 of N. helvetianus were used to re-establish a pure strain in calves, 2,3 million ova being recovered from infestations with 10 670 L 3 frozen for 26 months. The infectivity of the progeny of frozen L 3 was tested with M. marshalli and C. ovina. In both instances infectivity was high and the worms which developed also produced ova, thus completing the cycle. This appears to be the first report of infective larvae of parasitic nematodes retaining their infectivity after being frozen in liquid nitrogen (gas phase) for longer than 2 years. This is also apparently the first time that M. marshalli T. colubriformis, T. falculatus, T. axei, N. spathiger, C... 相似文献
9.
Eighteen helminth-free lambs were randomly allocated to six groups of three. Each lamb was dosed with 3300 infective larvae pooled from two isolates of Nematodirus spathiger known to be benzimidazole resistant. One lamb from each group was treated with oral ivermectin, one with oral oxfendazole and one left untreated 21 days after infection. All lambs were humanely killed 14 days later and small intestine worm counts performed. No Nematodirus were found in the ivermectin-treated lambs. Nematodirus numbers were reduced by 13% in oxfendazole-treated lambs relative to the control lambs. 相似文献
10.
Horak IG 《The Onderstepoort journal of veterinary research》2003,70(3):175-186
At monthly intervals for periods ranging from 9 to 25 consecutive months between three and four Merino yearling-sheep and two Merino lambs on three farms in Eastern Cape Thornveld, and two Dorper yearling-sheep on a farm in Valley Bushveld were slaughtered and examined for helminths. The Merino sheep were infected with 15 nematode species, of which Haemonchus contortus, Nematodirus spathiger and Trichostrongylus spp. were the most numerous, and with four cestode species. The Dorper sheep were infected with 17 nematode species, of which Trichostrongylus rugafus and N. spathiger were the most numerous, and with two cestode species. Haemonchus contortus was most numerous in older sheep during the summer months and exhibited no tendency to over-winter as arrested fourth stage larvae. The largest numbers of N. spathiger were present in sheep on the most coastally located of the farms during autumn and winter; on a farm further inland during spring; and on an inland farm in Valley Bushveld during spring and summer. As Merino lambs progressed from birth to 9 months of age a larger proportion of their dwindling burdens of N. spathiger was present as fourth stage larvae. Dorper sheep in Valley Bushveld harboured most Teladorsagia circumcincta in May and from October to January. Trichostrongylus rugatus was most numerous in Merino sheep on one of the Thornveld farms during September, whereas all Trichostrongylus spp., including T. rugatus, were most numerous in Dorper sheep on the Valley Bushveld farm during January. 相似文献
11.
Hosking BC Stein PA Mosimann D Seewald W Strehlau G Kaminsky R 《Veterinary parasitology》2008,157(1-2):72-80
Monepantel is the first compound from the recently discovered amino-acetonitrile derivative (AAD) class of anthelmintics to be developed for use in sheep. Three dose determination studies were conducted in Australia and Switzerland to identify the minimum therapeutic dose of monepantel when formulated for the oral treatment of sheep to control fourth stage (L4) gastro-intestinal nematode larvae. In each study, sheep infected with the target nematodes (selected from Haemonchus contortus, Teladorsagia (Ostertagia) circumcincta, Teladorsagia trifurcata, Trichostrongylus axei, Trichostrongylus colubriformis, Trichostrongylus vitrinus, Cooperia curticei, Cooperia oncophora, Nematodirus battus, Nematodirus filicollis, Nematodirus spathiger, Chabertia ovina and Oesophagostomum venulosum) were treated with either 1.25, 2.5 or 5.0mg monepantel/kg liveweight. Following euthanasia and worm counting, efficacy was calculated against worm counts from untreated control groups. Monepantel proved highly effective at 2.5 and 5.0mg/kg, but was only moderately effective against some nematode species (L4 stage) at 1.25mg/kg. The results also confirmed that monepantel will effectively control L4 stages of nematodes resistant to at least some of the currently available broad-spectrum anthelmintic classes (macrocyclic lactone resistant strains were not included in the studies). It was concluded that 2.5mg/kg would be a suitable minimum dose rate for a commercial product. No adverse events related to treatment with monepantel were detected. 相似文献
12.
Sissay, M.M., Uggla, A. and Waller, P.J., XXXX. Prevalence and seasonal incidence of nematode parasites and fluke infections
of sheep and goats in eastern Ethiopia. Tropical Animal Health and Production, XXXX. A 2-year abattoir survey was carried out to determine the prevalence, abundance and seasonal incidence of gastro-intestinal
(GI) nematodes and trematodes (flukes) of sheep and goats in the semi-arid zone of eastern Ethiopia. During May 2003 to April
2005, viscera including liver, lungs and GI tracts were collected from 655 sheep and 632 goats slaughtered at 4 abattoirs
located in the towns of Haramaya, Harar, Dire Dawa and Jijiga in eastern Ethiopia. All animals were raised in the farming
areas located within the community boundaries for each town. Collected materials were transported within 24 h to the parasitology
laboratory of Haramaya University for immediate processing. Thirteen species belonging to 9 genera of GI nematodes (Haemonchus contortus, Trichostrongylus axei, T. colubriformis, T. vitrinus, Nematodirus filicollis, N. spathiger, Oesophagostomum
columbianum, O. venulosum, Strongyloides papillosus, Bunostomum trigonocephalum, Trichuris ovis, Cooperia curticei and Chabertia ovina), and 4 species belonging to 3 genera of trematodes (Fasciola hepatica, F. gigantica, Paramphistomum {Calicohoron} microbothrium and Dicrocoelium dendriticum) were recorded in both sheep and goats. All animals in this investigation were infected with multiple species to varying
degrees. The mean burdens of adult nematodes were generally moderate in both sheep and goats and showed patterns of seasonal
abundance that corresponded with the bi-modal annual rainfall pattern, with highest burdens around the middle of the rainy
season. In both sheep and goats there were significant differences in the mean worm burdens and abundance of the different
nematode species between the four geographic locations, with worm burdens in the Haramaya and Harar areas greater than those
observed in the Dire Dawa and Jijiga locations. Similar seasonal variations were also observed in the prevalence of flukes.
But there were no significant differences in the prevalence of each fluke species between the four locations. Overall, the
results showed that Haemonchus, Trichostrongylus, Nematodirus, Oesophagostomum, Fasciola and Paramphistomum species were the most abundant helminth parasites of sheep and goats in eastern Ethiopia. 相似文献
13.
Albendazole was highly efficacious in the removal of monospecific and mixed infection of Haemonchus contortus, Nematodirus spathiger, and Dictyocaulus filaria from sheep. A dose level of 5 mg/kg removed nearly all gastrointestinal nematodes, and 10 mg/kg removed all lungworms. Tapeworms of the genus Moniezia were completely removed by a dose level of 10 hg/kg. 相似文献
14.
Boomker J Horak IG Watermeyer R Booyse DG 《The Onderstepoort journal of veterinary research》2000,67(1):31-41
The numbers and species of helminths recovered from one black wildebeest, Connochaetes gnou, three eland, Taurotragus oryx, 18 mountain reedbuck, Redunca fulvorufula, one red hartebeest, AIcelaphus buselaphus and two springbok, Antidorcas marsupialis, in the Mountain Zebra National Park, Eastern Cape Province; two black wildebeest, two grey rhebuck, Pelea capreolus, two mountain reedbuck and four springbok in the Karoo National Park, Western Cape Province; two bontebok, Damaliscus pygargus dorcas, two eland, two gemsbok, Oryx gazella and two springbok in the West Coast National Park, Western Cape Province; and a single springbok on a farm near Bredasdorp, Western Cape Province, are recorded. Nematodes belonging to a total of 12 genera and 20 species were identified. A single cestode was also recovered. Sixteen new host associations are recorded for the nematodes and one for the cestode Moniezia benedeni. Nematodirus spathiger had the widest host spectrum and with the exception of black wildebeest, was collected from all the host species examined. 相似文献
15.
Hoby S Robert N Mathis A Schmid N Meli ML Hofmann-Lehmann R Lutz H Deplazes P Ryser-Degiorgis MP 《Veterinary parasitology》2007,148(3-4):341-345
Pathological examination of five adult chamois (Rupicapra r. rupicapra) found dead in two different regions from the Swiss Alps revealed pale mucous membranes and musculature, swollen spleen and haemoglobinuria. Histologically, haemosiderosis in the spleen and centrilobular hepatic necrosis were the predominant findings. On blood smears, small (approximately 0.84-1.47 microm), round to pyriform, peripherally located inclusions were present in the erythrocytes. PCR followed by sequencing of DNA extracted from blood or spleen of the infected animals revealed 99-100% identity of the amplified part of the 18S rRNA gene with GenBank entries attributed to Babesia divergens/Babesia capreoli. This is the first report of fatal Babesia infections in chamois raising the question of an emerging disease in this species. 相似文献
16.
Parasites are linked with their host in a trophic interaction with implications for both hosts and parasites. Interaction stretches from the host's immune response to the structuring of communities and the evolution of biodiversity. As in many species sex determines life history strategy, response to parasites may be sex-specific. Males of vertebrate species tend to exhibit higher rates of parasites than females. Sex-associated hormones may influence immunocompetence and are hypothesised to lead to this bias. In a field study, we tested the prediction of male biased parasitism (MBP) in free ranging chamois (Rupicapra rupicapra rupicapra), which are infested intensely by gastrointestinal and lung helminths. We further investigated sex differences in faecal androgen (testosterone and epiandrosterone), cortisol and oestrogen metabolites using enzyme immunoassays (EIA) to evaluate the impact of these hormones on sex dependent parasite susceptibility. Non-invasive methods were used and the study was conducted throughout a year to detect seasonal patterns. Hormone levels and parasite counts varied significantly throughout the year. Male chamois had a higher output of gastrointestinal eggs and lungworm larvae when compared to females. The hypothesis of MBP originating in sex related hormone levels was confirmed for the elevated output of lungworm larvae, but not for the gastrointestinal nematodes. The faecal output of lungworm larvae was significantly correlated with androgen and cortisol metabolite levels. Our study shows that sex differences in steroid levels play an important role to explain MBP, although they alone cannot fully explain the phenomenon. 相似文献
17.
Gastrointestinal tracts of 48 yearling Maine dairy cattle were examined (8 animals every 2 months) for 1 year. Adult and immature nematodes were identified and counted. Species recovered were Bunostomum phlebotomum, Cooperia mcmasteri, Cooperia oncophora, Cooperia pectinata, Cooperia punctata, Haemonchus placei, Nematodirus helvetianus, Oesophagostomum radiatum, Ostertagia circumcincta, Ostertagia lyrata, Ostertagia ostertagi, Trichostongylus axei, and Trichuris ovis. Strongyle parasitism was observed during every sampling period and the overall incidence was 93.7%. The most commonly encountered genera were Ostertagia spp (85.4%) and Cooperia spp (81.2%). Seasonal fluctuations in the worm burden of various nematodes were observed, with largest numbers seen in the fall. The incidence and degree of parasitism appeared to be related to environmental conditions. Proportions of adult and immature worms were shown to fluctuate with the time of year. Greatest numbers of immature worms were observed during the fall and winter. 相似文献
18.
An investigation into the helminth species present in cattle in the East Kimberley and Victoria River Districts of Northern Australia was conducted over a 2-year period based on bi-monthly total and differential worm counts in weaner cattle as well as on a post mortem survey of adult cattle sent for slaughter. The most important species found in young cattle were Haemonchus placei and Cooperia spp. Only small numbers of Bunostomum phlebotomum, Nematodirus spathiger, Oesophagostomum radiatum and Moniezia benedini were present. Calicophoron calicophorum and nodules of Onchocerca spp. and Oe. radiatum were recorded in adult cattle. Seasonal fluctuations in helminth parasite burdens are discussed in relation to temperature and rainfall and the results are interpreted in relation to the effects of helminthosis on present and future beef production in this area. 相似文献
19.
Two worm-free calves were allowed to graze on irrigated pasture with a naturally infected herd for each of 34 one-month periods from November 1979 to August 1982. After each grazing period, the calves were transferred to a cement-floored pen for 3 weeks and then were euthanatized and necropsied. Ostertagia ostertagi and Cooperia oncophora were the most prevalent species of nematodes recovered. Adults and larvae of Ostertagia spp and Cooperia spp were most numerous in winter and spring and least numerous during summer. The proportions of Ostertagia spp that were inhibited as fourth-stage larvae increased in late fall, peaked from March through April, and then decreased to low values during summer. The maximal inhibition in 1980, 1981, and 1982 was 72, 65, and 62%, respectively. The number of larval Cooperia spp was highest in winter months and, except for one grazing period when 55% of the Cooperia spp were larvae, the total numbers represented less than 15% of the nematode population during all grazing periods. Other nematodes encountered were Trichostrongylus axei, Haemonchus spp, O lyrata, and O occidentalis in the abomasum; C surnabata, C punctata, Nematodirus helvetianus, T colubriformis, and Bunostomum phlebotomum in the small intestine; and Oesophagostomum venulosum and Trichuris ovis in the large intestine. 相似文献
20.
Infective larvae of selected batched of the following nematode species from sheep and cattle were examined for survival and infectivity (by injection into either the abomasum, the duodenum, or the jugular vein) after having been stored in liquid nitrogen for 103-136 months: Haemonchus contortus, Haemonchus placei, Ostertagia circumcincta, Ostertagia ostertagi, Marshallagia marshalli, Cooperia spp., Trichostrongylus axei, Trichostrongylus colubriformis, Trichostrongylus falculatus, Nematodirus spathiger, Nematodirus helvetianus, Oesophagostomum columbianum, Oesophagostomum radiatum, Chabertia ovina and Dictyocaulus filaria. Excluding D. filaria, a mean of 97.7% of the ovine and 96.0% of the bovine nematode larvae were alive when thawed. Compared with previous investigations in this series, little or no reduction occurred with time in either the survival or the viability of the nematodes from cattle, or in the survival of those from sheep. In contrast, the larvae developed poorly in sheep, possibly owing to parenteral treatment of the animals with ivermectin at a dosage of 0.4 mg kg-1, either 6 or 8 days before they were infected. 相似文献